CN115463157A

CN115463157A - A composition for treating skin injury

Info

Publication number: CN115463157A
Application number: CN202211192224.8A
Authority: CN
Inventors: 华彦; 王凯; 许学林; 梁晓彤; 刘昊; 王祥和; 李永政; 赵停; 吴文斌; 刘莎莎; 王佳怡
Original assignee: Guangdong Academy of Forestry
Current assignee: Guangdong Academy of Forestry
Priority date: 2022-09-28
Filing date: 2022-09-28
Publication date: 2022-12-13

Abstract

The invention discloses a composition for treating skin injury. The invention provides application of pangolin scales to treatment of skin injury, and provides a composition for treating skin injury, which is prepared from pangolin scales in a gel form. Experimental results show that after the pangolin squama manitis hydrogel is used for treatment, the skin edema of mechanical injury of rats is obviously relieved, the granulation tissue hyperplasia of wound surfaces is obvious, the infiltration of inflammatory cells is reduced, and the pangolin squama manitis hydrogel has a better injury repair effect on mechanical skin injury; and the side effect is small, and the application value is high.

Description

A composition for treating skin injury

Technical Field

The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a composition for treating skin injury.

Background

Skin wounds are the more common diseases in clinic, generally expressed as skin tissue injuries, and serious possibility of causing systemic secondary infection and even death. Therefore, how to promote the wound to heal quickly and avoid secondary infection and complications is a hot problem of domestic and foreign research. In recent years, many natural medicines have achieved good clinical effects in promoting the healing of skin wounds. With the rapid development of molecular biology and the wide application of new clinical technologies, researchers at home and abroad attach more and more importance to the repair mechanism of natural medicines for treating skin wounds by external application.

The pangolin scales as a natural Chinese medicinal material can play the roles of detumescence, pus discharge, meridian dredging and the like clinically, and has good biocompatibility and degradability. Pangolin scales are recorded in pharmacopoeia as having unique effects of promoting blood circulation, removing scar, dredging channels and breast, reducing swelling, expelling pus, dispelling wind and dredging collaterals. However, there is no research on pangolin scales and wound healing.

Disclosure of Invention

The invention aims to provide a new application of pangolin scales, namely the application of the pangolin scales in treating skin injury; and provides a composition for treating skin injury in the form of gel prepared from squama Manis.

It is a first object of the present invention to provide the use of pangolin scales for the treatment of skin lesions. The experimental result of the invention shows that the pangolin scales have good protection effect on mechanically damaged cells and can reduce the inflammatory reaction of mechanically damaged skin.

Preferably, the treatment of skin damage is treatment of skin burns, cuts or abrasions.

The second purpose of the invention is to provide a composition for treating skin injury, which is a composition containing pangolin scales and acceptable pharmaceutical auxiliary materials for skin.

Preferably, the composition for treating skin injury is pangolin squama hydrogel, and is prepared by the following steps:

soaking coarse powder of squama Manis slice in water, decocting, filtering, collecting filtrate, repeating the above steps for several times, mixing filtrates, concentrating, adding appropriate amount of carbomer powder, stirring, and making into hydrogel.

Preferably, the pangolin nail sheet hydrogel is prepared by the following steps:

s1, mixing 1g of coarse powder of vinegar-processed pangolin scales and water: adding 5mL of coarse powder of vinegar-processed pangolin scales into water, soaking for 1h, heating and decocting with slow fire for 20min, filtering, and collecting filtrate;

s2, repeating the step S1 twice;

s3, combining the filtrates for 3 times, heating in a water bath, and concentrating the filtrate until the volume of the filtrate is 10mL of the coarse powder of the vinegar-processed pangolin scales added in the S1: 1g, adding 0.5g of carbomer powder into the filtrate while the mixture is hot, and stirring to prepare pangolin scales hydrogel.

Preferably, the pangolin scales hydrogel is stored at 4 ℃.

The invention has the following advantages and beneficial effects:

1. the pangolin squama Manis hydrogel has good repairing effect on mechanically damaged cells and animal models.

2. The pangolin scales hydrogel has no stimulation to normal skin.

3. The hydrogel of pangolin scales has stable physical and chemical properties.

4. The pangolin squama Manis hydrogel has good anti-inflammatory effect, and can reduce skin inflammation reaction of injured SD rat.

5. After the pangolin squama manitis hydrogel is used for treatment, the mechanical injury skin edema of a rat is obviously relieved, the granulation tissue hyperplasia of a wound surface is obvious, and the inflammatory cell infiltration is reduced. The pangolin squama manitis hydrogel has good injury repairing effect on mechanical skin injury.

6. After the pangolin scales are prepared into gel, the gel is convenient to use, is easy to apply, can obviously reduce the incidence and the progress of mechanical skin injury, has small side effect, and is mature.

Drawings

FIG. 1 shows pangolin squama Manis hydrogel promoting healing of full-thickness skin injury; fig. a is an image of a wound site taken on

days

0, 5, 10, 15, 20, and 25 post-surgery; panel B is a histogram of scores obtained according to Table 1.

FIG. 2 shows that squama Manis hydrogel can promote scald healing; fig. a is an image of a wound site taken on days 0, 7, 14, and 21 after scald; panel B is a histogram of scores obtained according to Table 1.

FIG. 3 shows that squama Manis slice hydrogel promotes scald healing; the graph shows HE staining results of corresponding skin tissues after 21days of different treatments.

Detailed Description

The following examples are further illustrative of the present invention and are not intended to be limiting thereof.

The following examples: carbomer powder (30 g, model 940) was purchased from luobutrin bio; SD male rats are purchased from the experimental animal center of Guangdong province and meet the ethical requirements of animal experiments in the experimental process; the positive control commercial drug of Jingwanhong ointment (20 g, the main ingredients of sanguisorba officinalis, rehmannia glutinosa, angelica sinensis and the like, the dosage of each time is 0.2 g) is purchased from Tianjin Dalantang Jingwanhong pharmaceutical Co., ltd. The coarse powder of the vinegar pangolin scales is presented from wild animal monitoring rescue center of forestry academy of sciences of Guangdong province, and the preparation method comprises the following steps: cleaning squama Manis, parching with vinegar by conventional method, extracting, and grinding into 40 mesh coarse powder.

Example 1: preparation of pangolin nail sheet hydrogel

The preparation method of the pangolin nail sheet hydrogel comprises the following steps: adding 10g of coarse powder of vinegar-processed squama Manis into 50mL of distilled water, soaking for 1h, decocting with slow fire for 20min, filtering with 100-mesh filter screen, and collecting filtrate; then repeating the steps of adding water, soaking, decocting and filtering twice to collect filtrate, and combining the filtrate for 3 times. Heating in water bath, concentrating to obtain filtrate with volume of 100mL, adding 0.5g carbomer powder while hot, stirring, making into 10% squama Manis slice hydrogel, and storing at 4 deg.C. The hydrogel has special fragrance of vinegar-processed squama Manis, and is yellowish and transparent.

The physicochemical property of the pangolin scale hydrogel is further detected, and the result proves that the pangolin scale hydrogel has good stability.

(1) The prepared pangolin nail sheet hydrogel is light yellow, the whole gel is uniform and fine, and the gel can be kept colloidal at normal temperature without drying and liquefaction.

(2) The pangolin scales hydrogel has good ductility and no bubbles.

(3) The pH value of the pangolin nail sheet hydrogel is detected by utilizing a Sartorius pH meter, the pH value of the pangolin nail sheet hydrogel is detected to be about 5.5, and the pH value is in the range of 5.0-7.0 of the surface pH value of the skin of a normal human body. The pH value of the pangolin nail sheet hydrogel is in a neutral range, and the pangolin nail sheet hydrogel is suitable for being applied on skin.

(4) A proper amount of test sample is placed on a glass slide and is coated into a thin layer, the area of the thin layer is equivalent to that of a cover glass, 3 pieces of test sample are coated, and particles larger than 180 mu m are not detected.

(5) The pangolin squama manitis hydrogel is put into a 50mL sterile centrifuge tube, sealed and placed in a refrigerator at the temperature of minus 20 ℃ for 24h and a thermostat at the temperature of 55 ℃ for 6h, and the gel is observed to be uniform and fine as a whole, is kept colloidal at normal temperature, and has no dry and liquefaction phenomena.

In conclusion, the pangolin nail sheet hydrogel prepared by the method has good stability.

Example 2

1. Establishment of SD male rat full-layer skin defect model

A full-thickness skin defect model was established in 21 male SD rats of about 250g, and after shaving the skin of the backs of the rats, a full-thickness skin notch having a major axis of about 4cm and a minor axis of about 3cm was created in the skin of the backs of the rats under aseptic conditions using surgical tissue scissors. All 21 rats survived, and deep muscles were visible in the wound on the back, indicating successful modeling.

2. Treatment effect of pangolin squama Manis hydrogel on full-thickness skin defect model

21 successfully modeled SD rats were randomly divided into three groups, of which 7 rats were selected from the non-treatment group, the commercial drug group and the group of the plates. Non-treatment groups disinfected wounds using iodophors alone; the commercial medicine group uses iodophor to disinfect wounds and then smears commercial medicines; after the wound was sterilized by iodophor, the pangolin nail hydrogel prepared in example 1 was applied to the nail patch. The administration is continued for 14d, 1 time daily. Wounds were photographed at 0, 5, 10, 15, 20 and 25days after treatment with commercial drugs or pangolin nail hydrogel, respectively (fig. 1A). The results showed that in the full dissection injury nail treatment group on day 5, the wound margins of the rats contracted rapidly and the blood crust had completely disappeared (FIG. 1A, days 5). In addition, the best wound healing accelerating ability was also shown on day 20 in the nail treatment group (fig. 1a, days 20). The rats in the group A had significantly reduced wound area when treated for 25days compared to the group with the commercial drug and significantly different from the untreated group (FIG. 1A, days 25). On the other hand, according to the scores (fig. 1B and table 2, corresponding to the scoring criteria in table 1, the evaluation criteria was designed from two major directions of wound evaluation and wound regression in order to determine the therapeutic effect of the drug on rat skin wounds), when 20days was reached, the group a had significant advantages in wound size and depth, wound epithelialization coverage, and the difference in scores was significant compared with the other two groups (fig. 1B, days 20). At 25days, the group A received a score of 14 points in multiple indices of wound assessment and wound outcome, suggesting good healing, and the score was significantly different from the non-treated group (FIG. 1B, 25days).

TABLE 1 evaluation criteria for healing effect of drug treatment on rat full-dissection skin injury

TABLE 2 healing efficacy of the drug for treating full dissection of skin lesions in rats

Example 3

1. Establishment of SD male rat scald skin model

A scald skin model was established for 21 male SD rats of about 250g, and after shaving the back skin of the rat, the skin was kept on the back skin of the rat for 30 seconds under aseptic conditions using an iron weight heated to 100 ℃ with boiling water, resulting in a scald wound of 1cm radius. All 21 rats survived, the skin of the scalded part was pale and raised, and blister scabs remained, indicating that the model building was successful.

2. Treatment effect of pangolin scales hydrogel on scalded skin model

21 successfully modeled SD rats were randomly divided into three groups, of which 7 rats were selected from the non-treatment group, the commercial drug group and the group of the plates. Non-treatment groups disinfected wounds using iodophors alone; the commercial medicine group uses iodophor to disinfect wounds and then smears commercial medicines; after the wound was sterilized by iodophor, the pangolin nail hydrogel prepared in example 1 was applied to the nail patch. The administration is continued for 14d, 1 time daily. Wounds were photographed at 0, 7, 14, and 21days after treatment with commercial drugs or pangolin nail hydrogel, respectively. Experimental observations have shown that the epidermal crusts of the rats in the nail plate group were detached at 14days of scald treatment, the inner part healed well (fig. 2a, days 14), and the wound healed almost completely after 21days (fig. 2a, days 21), with a significant difference compared to the non-treated group (fig. 2a, days 21). On the other hand, according to the scores (fig. 2B and table 4, corresponding to the scoring criteria in table 3, the evaluation criteria were designed from two major directions of wound evaluation and wound regression in order to determine the therapeutic effect of the drug on rat skin wounds), when 14days, the group a had significant advantages in wound size and depth, wound epithelialization coverage, and the difference in scores was significant compared with the other two groups (fig. 2b, day 14). When 21days was observed, the group A had scores of 14 points among multiple indicators of wound assessment and wound regression, suggesting that the healing was good and the scores were significantly different from those of the untreated group (FIG. 2B, 21days).

TABLE 3 evaluation standard of healing effect of drug for treating scald skin injury of rat

TABLE 4 healing effect of the drugs on scald and skin injury in rats with fine score

3. Histopathological section analysis after scald treatment

After scald treatment, pathological section analysis is carried out, the skin integrity of a pathological picture observation nail plate group (pangolin nail hydrogel treatment group) can be better divided into 3 layers, namely epidermis, dermis and subcutaneous tissues, and the obvious 3 layers of skin tissues can be seen in a commercial medicine group, but the epidermis completeness is slightly poor, but the epidermis and the dermal tissues of a non-treatment group have obvious defects (figure 3).

Claims

1. Application of squama Manis and squama Manis in treating skin injury is provided.

2. The use of claim 1, wherein said skin injury treatment is treatment of a scald, a cut or an abrasion of the skin.

3. A composition for treating skin injury is characterized by comprising pangolin scales and acceptable pharmaceutical excipients for skin.

4. The composition for treating skin lesion of claim 3, wherein the composition for treating skin lesion is pangolin squama Manis hydrogel, which is prepared by the following steps: soaking coarse powder of squama Manis slice in water, decocting, filtering, collecting filtrate, repeating the above steps for several times, mixing filtrates, concentrating, adding appropriate amount of carbomer powder, stirring, and making into hydrogel.

5. The composition for treating skin lesion according to claim 4, wherein the hydrogel of pangolin scales is prepared by the following steps:

s1, mixing 1g of coarse powder of vinegar-processed pangolin scales and water: adding the coarse powder of the vinegar-processed pangolin scales into water in a proportion of 5mL, soaking for 1h, heating and decocting with slow fire for 20min, filtering and collecting filtrate;

s2, repeating the step S1 twice;