CN106421160A - Wound-cleaning traditional Chinese medicine composition for anal fistula as well as preparation method and application of traditional Chinese medicine - Google Patents
Wound-cleaning traditional Chinese medicine composition for anal fistula as well as preparation method and application of traditional Chinese medicine Download PDFInfo
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Abstract
The invention discloses a wound-cleaning traditional Chinese medicine composition for anal fistula as well as a preparation method and application of the traditional Chinese medicine. The traditional Chinese medicine comprises the following components in parts by weight: 50-100 parts of climbing groundsel herb, 50-100 parts of dandelion, 40-60 parts of wild chrysanthemum flower and 40-60 parts of herba verbenae. The traditional Chinese medicine composition has the functions of clearing away heat and toxic materials, astringing exudation, eliminating dampness, diminishing inflammation, relieving pain, removing necrotic tissues and promoting granulation and has remarkable effects in the preparation of medicines for treating pressure sores of the anal fistula.
Description
Technical field
The invention belongs to technical field of traditional Chinese medicines, it is related to a kind of anal fistula debridement Chinese medicine composition and its preparation method and application,
Said composition is mainly used in the cleaning of the Crohn disease anal fistula surface of a wound, accelerates the exclusion of surface of a wound fester, promotes the healing of the surface of a wound.
Background technology
Crohn disease (Crohn ' s disease, CD) is the chronic inflamatory that a kind of cause of disease is still not clear, and anal fistula is
Common complication, incidence is up to 50%, and easy recurrent exerbation.Surgical operation is essential therapeutic arsenals, special due to position
Property, repair with repeatedly polluting and deposit, lead to wound healing slow.Clinical shortage topical cleansers, can only be entered with physiological saline
Row cleaning, patient is painful, and doctor has a headache.It is clinical refractory disease.
Content of the invention
The purpose of the present invention is to overcome above-mentioned weak point to provide a kind of anal fistula debridement Chinese medicine composition.
It is a further object of the present invention to provide the preparation method of this anal fistula debridement Chinese medicine composition.
The purpose of the present invention is achieved in the following ways：
A kind of anal fistula debridement Chinese medicine composition, this Chinese medicine composition is made up of the component of following weight portion：
Climbing groundsel 50～100 weight portion, dandelion 50～100 weight portion, mother chrysanthemum 40～60 weight portion, Verbena officinalis 40～
60 weight portions.
Above-mentioned anal fistula debridement Chinese medicine composition is preferably made up of the component of following weight portion：
Climbing groundsel 70～80 weight portion, dandelion 70～80 weight portion, mother chrysanthemum 40～60 weight portion, Verbena officinalis 40～60
Weight portion.
The preparation method of above-mentioned anal fistula debridement Chinese medicine composition comprises the following steps：
By climbing groundsel, dandelion, mother chrysanthemum and the mixing of Verbena officinalis 4 taste medicinal material, decocting boils 2～3 times, 1～2 hour every time,
Collecting decoction, filters, obtains extract, and extract is concentrated into medicinal material and extract weight ratio for 1：When 1～2, cooling, plus carry
Take liquid 2～3 times amount mass concentration be more than 95% ethanol, precipitate more than 24 hours, filtration, reclaim ethanol to no alcohol taste.
Borax can also be added in above-mentioned anal fistula debridement Chinese medicine composition.
Concrete combination is as follows：
Climbing groundsel 50～100 weight portion, dandelion 50～100 weight portion, mother chrysanthemum 40～60 weight portion, Verbena officinalis 40～
60 weight portions, borax 15～25 weight portion.
Further preferably anal fistula debridement Chinese medicine composition is：
Climbing groundsel 70～80 weight portion, dandelion 70～80 weight portion, mother chrysanthemum 40～60 weight portion, Verbena officinalis 40～60
Weight portion, borax 15～25 weight portion.
The preparation method of above-mentioned anal fistula debridement Chinese medicine composition comprises the following steps：
By climbing groundsel, dandelion, mother chrysanthemum and the mixing of Verbena officinalis 4 taste medicinal material, decocting boils 2～3 times, 1～2 hour every time,
Collecting decoction, filters, obtains extract, and extract is concentrated into medicinal material and extract weight ratio for 1：When 1～2, cooling, plus carry
Take liquid 2～3 times amount mass concentration be more than 95% ethanol, precipitate more than 24 hours, filtration, reclaim ethanol to no alcohol taste,
Again plus borax mixing.
External preparation made by above-mentioned Chinese medicine composition and pharmaceutically acceptable auxiliary material, and described external preparation is preferably washed
Agent.
Anal fistula debridement lotion of the present invention is specifically made up of the component of following weight portion：
Climbing groundsel 50～100 weight portion, dandelion 50～100 weight portion, mother chrysanthemum 40～60 weight portion, Verbena officinalis 40～
60 weight portions, glycerine 30～60 weight portion, then add pure water to 180g-320g crude drug/(crude drug comprises a thousand li to 500-1000ml
Light, dandelion, mother chrysanthemum and Verbena officinalis).
The preparation method of above-mentioned anal fistula debridement washing lotion comprises the following steps：
By climbing groundsel, dandelion, mother chrysanthemum and the mixing of Verbena officinalis 4 taste medicinal material, decocting boils 2～3 times, 1～2 hour every time,
Collecting decoction, filters, obtains extract, and extract is concentrated into medicinal material and extract weight ratio for 1：When 1～2, cooling, plus carry
Take liquid 2～3 times amount mass concentration be more than 95% ethanol, precipitate more than 24 hours, filtration, reclaim ethanol to no alcohol taste,
Glycerol adding and pure water mix to full dose again.
The present invention borated anal fistula debridement lotion is specifically made up of the component of following weight portion：
Climbing groundsel 50～100 weight portion, dandelion 50～100 weight portion, mother chrysanthemum 40～60 weight portion, Verbena officinalis 40～
60 weight portions, borax 15～25 weight portion, glycerine 30～60 weight portion, then add pure water to 180g-320g crude drug/500--
1000ml (crude drug comprises climbing groundsel, dandelion, mother chrysanthemum and Verbena officinalis).
The preparation method of above-mentioned anal fistula debridement washing lotion comprises the following steps：
By climbing groundsel, dandelion, mother chrysanthemum and the mixing of Verbena officinalis 4 taste medicinal material, decocting boils 2～3 times, 1～2 hour every time,
Collecting decoction, filters, obtains extract, and extract is concentrated into medicinal material and extract weight ratio for 1：When 1～2, cooling, plus carry
Take liquid 2～3 times amount mass concentration be more than 95% ethanol, precipitate more than 24 hours, filtration, reclaim ethanol to no alcohol taste,
Again plus borax, glycerine and pure water mix to full dose.
The using method of above-mentioned washing lotion：External application, in right amount, local painting is put on the skin, soak or surface of a wound cleaning (can use warm water if necessary
1 times of dilution), 2 times a day or follow the doctor's advice.
Anal fistula debridement Chinese medicine composition prescription of the present invention is mainly by climbing groundsel, dandelion, mother chrysanthemum, Verbena officinalis
Composition, has clearing heat and detoxicating, restrains eliminating dampness, anti-inflammatory analgetic, the function of removing necrosis and promoting granulation, in the medicine of preparation treatment anal fistula pressure sore
Application has significant effect.The washing lotion that this prescription is made is used for anal fistula pressure sore, infects the surface of a wound cleaning based on oozing out.
Through extracorporeal bacteria inhibitor test prove to staphylococcus aureus, EHEC, pseudomonas aeruginosa, enterococcus faecalis,
Candida albicans all has obvious fungistatic effect, and is significantly better than alone borax preparation.
Animal experiment proves that the surface of a wound of the trauma surface infestation to bacterium infection for the anal fistula debridement Chinese medicine composition of the present invention has control
Infection promotes the effect of wound healing.Clinical principium is applied, satisfactory effect.
Specific embodiment
Below by way of specific embodiment, the present invention is further described：
Embodiment 1
Climbing groundsel 80g, dandelion 70g, mother chrysanthemum 40g, Verbena officinalis 60g, borax 15g, glycerine 40g.
Preparation method：Take climbing groundsel, dandelion, mother chrysanthemum, Verbena officinalis 4 taste medicinal material mixes, decocting 3 times, 2 hours every time,
Collecting decoction, filters to obtain extract, and extract is concentrated into medicinal material and extract weight than for 1：When 1, cooling, plus percent mass
Concentration be 95% ethanol to 3 times of weight of extract, precipitate more than 24 hours, filtration, reclaim ethanol to no alcohol taste, plus boron
Sand, glycerine and pure water are to 1000ml full dose.
Embodiment 2
Climbing groundsel 75g, dandelion 75g, mother chrysanthemum 60g, Verbena officinalis 40g, borax 20g, glycerine 45g.
Preparation method：Take climbing groundsel, dandelion, mother chrysanthemum, Verbena officinalis 4 taste medicinal material mixes, decocting 2 times, 2 hours every time,
Collecting decoction, is concentrated into medicinal material and extract weight than for 1：When 2, cooling, plus 2 times of the ethanol that mass percentage concentration is 95%
Amount, precipitates more than 24 hours, filtration, reclaims ethanol to no alcohol taste, plus borax, glycerine and pure water are to 1000ml full dose.
Embodiment 3
Climbing groundsel 70g, dandelion 80g, mother chrysanthemum 50g, Verbena officinalis 50g, borax 18g, glycerine 50g.
Preparation method：Take climbing groundsel, dandelion, mother chrysanthemum, Verbena officinalis 4 taste medicinal material mixes, decocting 3 times, 1 hour every time,
Collecting decoction is concentrated into liquid weight than for 1：When 1, cooling, plus ethanol 2 times amount that mass percentage concentration is 95%, precipitation 24 is little
When more than, filtration, reclaim ethanol to no alcohol taste, plus borax, glycerine and pure water are to 1000ml full dose.
Embodiment 4
Climbing groundsel 80g, dandelion 70g, mother chrysanthemum 40g, Verbena officinalis 60g, glycerine 40g.
Preparation method：Take climbing groundsel, dandelion, mother chrysanthemum, Verbena officinalis 4 taste medicinal material mixes, decocting 3 times, 2 hours every time,
Collecting decoction, filters to obtain extract, and extract is concentrated into medicinal material and extract weight than for 1：When 1, cooling, plus percent mass
Concentration be 95% ethanol to extract 3 times of weight, precipitate more than 24 hours, filtration, reclaim ethanol to no alcohol taste, glycerol adding
And pure water is to 1000ml full dose.
Test example 1：Seen with the clinical principium that embodiment 4 debridement washing lotion has carried out drug combination to Crohn disease anal fistula patient
Examine, each 15 for the treatment of group, control group.As comparison, by clinical observation, conventional therapy merges Chinese medicine debridement and washes 2% borax soln
Liquid treats Crohn disease anal fistula, and cure rate significantly improves, and treatment time greatly shortens, and recurrence rate is low, and patient compliance is good, faces
Bed application advantage substantially, the results are shown in Table 1.
The impact that table 1 debridement washing lotion heals to Crohn disease anal fistula
Note：With compare,#p<0.05,##p<0.01
Test example 2：With the shadow to bacterial infection wound healing for embodiment 1 product (for debridement washing lotion group in tests below)
Ring
1. experiment material
1.1 animal：Cleaning grade ICR mouse, body weight 20g～25g, male and female half and half, carried by Yangzhou University's comparative medicine center
For animal used as test production licence：SCXK (Soviet Union) 2012-0004.
1.2 test medicine：Debridement washing lotion (prepares according to embodiment 1 method)；Debridement washing lotion stoste is (according to embodiment
4 methods prepare, and remaining, with embodiment 1, does not only contain borax)；Positive control (2% borax soln).
1.3 medicine preparations, dosage, packet and administering mode：
Medicine is directly using it is not necessary to process through dilution.Directly rinse the method administration of wounds in mice, punching using liquid
The standard of washing is to rinse the fester of wounds in mice, slough etc. well.
Test mice underwent operative infection staphylococcus aureus or Escherichia coli, prepare bacterial infection wound model.
After modeling success, animal is randomly divided into model group, negative control group, positive controls, debridement washing lotion, debridement washing lotion stoste
Group.Wherein, do not carry out any type for the treatment of after model group modeling success；Using life after the animal model success of negative control group
Reason normal saline washing wounds in mice, morning and afternoon is respectively once；Positive controls use 2% borax soln to rinse wounds in mice, morning and afternoon
Respectively once；Traditional Chinese medicine lotion treatment group uses corresponding traditional Chinese medicine lotion to rinse wounds in mice according to group respectively, and morning and afternoon is respectively once.
2. test method
2.1.1 impact experiment infection of staphylococcus aureus wounds in mice repaired
Trauma surface infestation modeling experiment starts the previous day, inoculates staphylococcus aureus, 37 degree of lower trainings in superclean bench
Support overnight.Bacterium solution by incubated overnight is inoculated in fresh LB fluid nutrient medium according to 1: 100 within second day, trains under the conditions of 37 degree
Support to OD600=0.6～0.8.In 50ml centrifuge tube, 3000rpm is centrifuged 10 minutes the bacterium solution collecting culture, abandoning supernatant,
Subsequently with fresh LB fluid nutrient medium, bacterium mud is suspended, and bacterial concentration is diluted to 5 × 1011Individual/ml, bacterium solution is placed in refrigerator
Standby.
Choose SPF level mouse 70, male and female half and half, body weight 20-25g, anaesthetized with yellow Jackets.Mouse prone position is solid
Fixed, strike off mouse back hair with double-edged razor blade, subsequently use 75% alcohol disinfecting.In back nearly mouse hind leg position with backbone it is
Center line excises the full thickness skin of diameter about 1cm, and is drawn, with syringe needle, the surface of a wound excised after full thickness skin by " well " font
Wound, the surface of a wound exposes with after antiseptic gauze hemostasis.The standby staphylococcus aureus bacterium solution of 20 μ l is inoculated at the surface of a wound, every to ensure
The trauma surface infestation 1 × 10 of mouse10Individual staphylococcus aureus.Infection 2 times daily, for three days on end, until causing local infection,
Red and swollen around wound, the surface of a wound has fester or oozes out.Random mouse is 5 groups, that is, model group, negative control group (physiological saline),
Positive controls (2% BAS), debridement washing lotion group, debridement washing lotion stoste group.Each group rinses wound with corresponding washing lotion daily
Face 2 times, morning and afternoon respectively once, continuous 12 days.Created with membrane process measurement back respectively within 1st, 3,5,7,9,11 day in drug treatment
Face area.Use the transparent plastic mulching surface of a wound, draw film along edge of wound, cut film, calculate surface of a wound area, and shrinkage from mold dimensions (refers to formula
1).Put to death mouse after last dose, cut surface of a wound skin, do histotomy after formalin fix, after HE dyeing, observe wound
Face epithelium rate, evaluates wound healing situation.
2.1.2 impact experiment coli-infection wounds in mice repaired
Experimental program is with infection of staphylococcus aureus wounds in mice reparative experiment.The bacterium of infection modeling is changed to large intestine bar
Bacterium, the packet of animal used as test, therapeutic scheme and inspection target are tested with 2.1.1.
2.1.3 the histology experiment to the impact that bacterial infection wounds in mice is repaired
Take and gather in 2.1.1 and 2.1.2 experiment and be stored in the skin histology in 10% formalin solution, routine paraffin wax
Embedding, 4～5 μm of slice thick, HE dyes, and observation by light microscope simultaneously scores.Inspection project and score by rules are as follows：
(1) epidermal cell has or not degeneration necrosis, forms rotten to the corn, ulcer, crust：It is chosen as 0 point～4 points.0 point of no necrosis, by
Light arriving is 1 point～4 points again；(eyepiece 4 ×, the object lens 10 ×) measurement under 40 times of light microscopics with " the microscope micro- chi in side " of downright bad length
The length (cm/40 times) of ulcer.
(2) below slough, corium and hypodermis blood vessel have or not expansion, hyperemia, oedema, cell infiltration：It is chosen as 0
Divide～4 points.0 point of no obvious pathology, 1 point～4 points lesion degrees are respectively slight, moderate, severe, pole severe；
(3) granulation tissue hyperplasia or fibrillatable：Divide 0 point～3 points, 0 point：Ripe granulation tissue, complete fibrillatable；3 points of nothings
Granulation tissue, no fibroblast proliferation；1 point：Granulation tissue is in early stage in the inmature stage, and fibrillatable is inconspicuous；2 points：Slightly
Maturation, vasculitis Leukopenia in granulation tissue, fibroblast proliferation.
(4) damage surrounding epithelial tissue and have or not hyperplasia：It is divided into 0 point～3 points.Damage both sides no epithelium and be regenerated as 3 points, 1 side
Epithelium regeneration is had to be 2 points, it is 1 point that bilateral has epithelium regeneration, bilateral has epithelium regeneration in central portion joint or closely to combine for 0
Point.
3. result
The impact that 3.1 pairs of infection of staphylococcus aureus wounds in mices are repaired
As it can be seen from table 1 negative control group is slow with the wound healing of model group, saline therapy is for golden yellow
The healing of staphy lococcus infection wounds in mice does not have facilitation.And 2% borax soln then can be obviously promoted mouse wound
Heal in face, the 3rd after treatment day start, and the surface of a wound of positive controls mouse is obviously reduced, shrinkage factor apparently higher than model group with
Negative control group.The therapeutic action of this wound healing to the 5th day seems more notable (p<0.01).In tested liquid,
Debridement washing lotion stoste has preferable therapeutic action, can significantly reduce the surface of a wound (p within the 3rd day after treatment<0.05), treat
The 7th day afterwards, the effect of this wound healing was remarkably reinforced (p<0.01).Debridement washing lotion is to infection of staphylococcus aureus
The facilitation of wound healing emerges from after treatment on the 7th day.
The impact that 3.2 pairs of coli-infection wounds in mices are repaired
From table 2 it can be seen that negative control group is slow with the wound healing of model group, saline therapy is for large intestine bar
The healing of bacterium infectivity wounds in mice does not have facilitation.And 2% borax soln then can be obviously promoted wounds in mice healing,
The 3rd day after treatment starts, and the surface of a wound of positive controls mouse has the effect of reducing, and shrinkage factor is apparently higher than model group (p<
0.05), the therapeutic action of this wound healing is continued for terminating to experiment.In tested liquid, debridement washing lotion stoste has
Preferably therapeutic action, can significantly improve the shrinkage factor (p of the surface of a wound after treatment on the 3rd day<0.01), and whole test
It all can be observed in cycle and significantly promote the effect of wound healing.Debridement washing lotion is to coli-infection mouse
The pathological analysis that 3.3 debridement washing lotions affect on bacterial infection wounds in mice reparation
3.3.1 wound infection S. aureus L-forms pathological examination
8 mouse of model group all have ulcer different in size, skin surface visible solid, organized and oozed by inflammatory necrosis
Go out the crust of composition, the visible a large amount of inflammatory cells in bottom, granulation tissue is inmature, and there is epithelial tissue part ulcer edge 1 side or 2 sides again
Raw.8 mouse local skins are shown in ulcer different in size, average length 1.67cm/40 times.See have thick-layer to add scab at 8 ulcer
Skin, wherein 6 is severe, 1 pole severe, 1 moderate.Moderate cell infiltration is arranged at 1 ulcer bottom, and 7 is severe or pole
Severe, inflammatory cell type is mainly neutrophil leucocyte.4 ulcer bottoms have no obvious granulation tissue, and 2 is inmature granulation group
Knit (granulation tissue is made up of, and inside has cell infiltration) newborn capillary and fibroblast.Blood in another 2 granulation tissues
Pipe quantity reduces, and collagenous fibres increase, slightly ripe.No epithelium regeneration around 4 ulcer, a small amount of epithelium regeneration is seen in 21 sides,
There is epithelium regeneration another 22 sides, extend to ulcer center.
8 mouse of negative control group all have ulcer different in size, skin surface visible solid, organized by inflammatory necrosis
With the crust oozing out composition, the visible a large amount of inflammatory cells in bottom and inmature granulation tissue, most ulcer edges no epithelial tissue is again
Raw.8 mouse local skins are shown in ulcer different in size, average length 1.54cm/40 times.See have thick-layer to add scab at 8 ulcer
Skin, wherein 5 is severe, 2 moderates, and 1 slight.Ulcer bottom 1 only has pole severe cell infiltration, 7 severes.Inflammatory cell
The same model group of type.3 ulcer bottoms have no obvious granulation tissue, and 5 is inmature granulation tissue.Supreme around 5 ulcer
Skin regenerates, and the epithelium of a small amount of regeneration is seen in 31 sides.
8 murine lesion of positive group are little compared with model group, negative control group, and skin surface crust reduces, ulcer bottom granulation
Two groups of maturations are also relatively gone up in hyperblastosis, and epithelium regeneration increases.8 mouse local skins are shown in ulcer different in size, average length
1.38cm/40 again.See there is crust at 8 ulcer, wherein 2 moderates, 5 are slight, and 1 is inconspicuous.Ulcer bottom 6 severe inflammation
Cellular infiltration, 2 moderates.The same model group of inflammatory cell type.1 ulcer bottom has no obvious granulation tissue, and 5 is inmature meat
Bud is organized, and in 2 granulation tissues, fibroblast is increased, blood vessel reduces, more ripe.No epithelium regeneration around 2 ulcer, 61
The epithelium of a small amount of regeneration is seen in side.
Debridement 8 mouse lesion degrees of washing lotion stoste group substantially mitigate compared with model group and negative control group.7 mouse local
Skin is shown in ulcer different in size, average length 1.07cm/40 times.5 only have degree of taking a favourable turn crust at ulcer, 3 no obvious crusts.
Severe cell infiltration is arranged at 4 ulcer bottoms, and 3 slight, 1 no obvious cell infiltration.The same model group of inflammatory cell type.1
Ulcer bottom no granulation tissue hyperplasia, the granulation tissue of naivety is seen in 4 ulcer bottoms, and 1 granulation tissue is more ripe.Burst for 1
No epithelium regeneration around ulcer, the epithelium of a small amount of regeneration is seen in 21 sides, and epithelium regeneration is seen in 42 sides.
8 mouse lesion degrees of debridement washing lotion group slightly mitigate compared with model group and negative control group.7 mouse local skins are shown in
Ulcer different in size, average length 1.77cm/40 times.7 only see crust, wherein 5 moderates at ulcer, and slight or severe is each
1.Severe cell infiltration arranged at 7 ulcer bottoms, 1 no at ulcer on subcutaneous have moderate inflammatory cell.The same mould of inflammatory cell type
Type group.The granulation tissue of naivety is seen in 6 ulcer bottoms, and in 1 granulation tissue, fibroblast is increased, blood vessel reduces, more ripe.
No epithelium regeneration around 5 ulcer, the epithelium of a small amount of regeneration is seen in 21 sides.
Each group pathological score statistic analysis result refers to table 3.
3.3.2 wound infection Escherichia coli pathology result
8 mouse of model group all have ulcer different in size, skin surface visible solid, organized and oozed by inflammatory necrosis
Go out the crust of composition, the visible a large amount of inflammatory cells in bottom, most mouse granulation tissue is slight, and degree has with ulcer edge 1 side or 2 sides
Epithelial tissue regenerates.8 mouse local skins are shown in ulcer different in size, average length 1.16cm/40 times.See at 8 ulcer
There is crust, wherein 1 is severe, 3 moderates, and 4 slight.Moderate cell infiltration arranged at 1 ulcer bottom, 7 be severe or
Pole severe, inflammatory cell type is mainly neutrophil leucocyte.Inmature granulation tissue is seen in 1 ulcer bottom, and (granulation tissue is by new life
Capillary and fibroblast composition, inside have cell infiltration).In another 7 granulation tissues, blood vessel number reduces, and collagen is fine
Dimension increases, slightly ripe.No epithelium regeneration around 2 ulcer, a small amount of epithelium regeneration is seen in 31 sides, and there is epithelium another 32 sides again
Raw.
8 mouse of negative control group all have ulcer different in size, skin surface visible solid, organized by inflammatory necrosis
With the crust oozing out composition, the granulation tissue of the visible a large amount of inflammatory cells in bottom and naivety, part ulcer edge is shown in epithelial tissue again
Raw.8 mouse local skins are shown in ulcer different in size, average length 1.09cm/40 times.See there is crust, wherein at 8 ulcer
4 moderates, 3 slight, and 1 inconspicuous.Ulcer bottom 1 only has pole severe cell infiltration, 7 severes.Inflammatory cell type is same
Model group.6 ulcer bottoms have no obvious granulation tissue, and 2 is inmature granulation tissue.No epithelium regeneration around 2 ulcer,
The epithelium of a small amount of regeneration is seen in 21 sides, and there is epithelium regeneration 42 sides.
Positive 8 mouse skin degree of injury of group substantially mitigate compared with model group and negative control group.7 mouse local skins
See ulcer different in size, average length 1.09cm/40 times.See there is crust at 7 ulcer, wherein 2 moderates, 5 slight or
Slightly, 1 epithelial surface degree of taking a favourable turn crust.Ulcer bottom 6 severe cell infiltration, 2 moderates.The same model of inflammatory cell type
Group.3 is inmature granulation tissue, and 5 granulation tissues are more ripe.Around 2 ulcer, the epithelium of a small amount of regeneration is seen in 1 side, 52
There is epithelium regeneration side, and 1 skin surface covers for epithelium, but is not fully connected.
Debridement 8 mouse lesion degrees of washing lotion stoste group substantially mitigate compared with model group and negative control group.7 mouse local
Skin is shown in ulcer different in size, average length 1.18cm/40 times.3 only see moderate crust, 2 slight crusts at ulcer.Burst
Ulcer bottom severe or each 4 of moderate cell infiltration, the same model group of inflammatory cell type.1 ulcer bottom no granulation tissue hyperplasia,
The granulation tissue of naivety is seen in 4 ulcer bottoms, and 2 granulation tissues are more ripe.No epithelium regeneration around 1 ulcer, 21 sides are seen
The epithelium of a small amount of regeneration, epithelium regeneration is seen in 52 sides.
8 mouse lesion degrees of debridement washing lotion group mitigate compared with model group and negative control group.7 mouse local skins are good at
The short ulcer differing, average length 1.13cm/40 times.4 only see crust, wherein 2 moderates, slight or severe each 1 at ulcer
Only.Pole severe or severe cell infiltration are arranged at 6 ulcer bottoms, and 2 is moderate inflammatory cell.The same model group of inflammatory cell type.5
The granulation tissue of naivety is seen in ulcer bottom, and 2 granulation tissues are more ripe.No epithelium regeneration around 1 ulcer, 21 sides are seen on a small quantity
The epithelium of regeneration, the epithelium of regeneration is seen in 52 sides.
Each group pathological score statistic analysis result refers to table 3.
Table 3 mouse skin pathology light and heavy degree appraisal result ()
| Group | Number of cases | Infection S. aureus L-forms group | Ehec infection group |
| Model group | 8 | 10.06±3.28 | 8.50±2.84 |
| Negative control group | 8 | 10.63±1.41 | 9.0±1.51 |
| Positive group | 8 | 8.00±2.59** | 6.13±2.09** |
| Debridement washing lotion stoste | 8 | 6.00±2.17** | 6.25±2.25* |
| Debridement washing lotion group | 8 | 8.63±2.77 | 6.88±2.53* |
Note：Each group and negative control group ratio：* P ＜ 0.05, * * P ＜ 0.01
4. conclusion
Mouse bacteria infection damages pathology and shows as：Ulcer in skin histology, and skin surface is visible solid, by inflammation
Property slough and exudate composition crust, the visible a large amount of inflammatory cells in bottom, part ulcer bottom is looked into and is seen granulation tissue hyperplasia,
The visible epithelial tissue of ulcer edge regenerates.Comparing with normal group has statistical significant difference, and skin trauma model copy is described
Success.
Anal fistula debridement Chinese medicine composition of the present invention all can effectively reduce staphylococcus aureus or large intestine bar in treatment phase
The area of the bacterium infecting mouse surface of a wound, significantly improves the shrinkage factor of the surface of a wound, and the healing to the surface of a wound has facilitation.From experimental data
Analysis, the healing to infection of staphylococcus aureus wounds in mice has significant facilitation, little to coli-infection
The facilitation of mouse wound healing is the most obvious.
Claims (13)
1. a kind of anal fistula debridement Chinese medicine composition is it is characterised in that this Chinese medicine composition is made up of the component of following weight portion：
Climbing groundsel 50～100 weight portion, dandelion 50～100 weight portion, mother chrysanthemum 40～60 weight portion, Verbena officinalis 40～60 weight
Amount part.
2. anal fistula debridement Chinese medicine composition according to claim 1 is it is characterised in that this Chinese medicine composition is by following weight
The component of part is made：
Climbing groundsel 70～80 weight portion, dandelion 70～80 weight portion, mother chrysanthemum 40～60 weight portion, Verbena officinalis 40～60 weight
Part.
3. a kind of anal fistula debridement Chinese medicine composition is it is characterised in that this Chinese medicine composition is made up of the component of following weight portion：
Climbing groundsel 50～100 weight portion, dandelion 50～100 weight portion, mother chrysanthemum 40～60 weight portion, Verbena officinalis 40～60 weight
Amount part, borax 15～25 weight portion.
4. anal fistula debridement Chinese medicine composition according to claim 3 is it is characterised in that this Chinese medicine composition is by following weight
The component of part is made：
Climbing groundsel 70～80 weight portion, dandelion 70～80 weight portion, mother chrysanthemum 40～60 weight portion, Verbena officinalis 40～60 weight
Part, borax 15～25 weight portion.
5. the Chinese medicine composition according to claim 1-4 any one claim is it is characterised in that said composition and medicine
Learn acceptable auxiliary material and make external preparation.
6. Chinese medicine composition according to claim 5 is it is characterised in that described external preparation is lotion.
7. a kind of preparation method of the anal fistula debridement Chinese medicine composition described in claim 1 or 2 is it is characterised in that the method includes
Following steps：
By climbing groundsel, dandelion, mother chrysanthemum and the mixing of Verbena officinalis 4 taste medicinal material, decocting boils 2～3 times, 1～2 hour every time, merges
Decocting liquid, filters, obtains extract, and extract is concentrated into medicinal material and extract weight ratio for 1：When 1～2, cooling, plus extract
The mass concentration of 2～3 times amount is more than 95% ethanol, precipitates more than 24 hours, filtration, reclaims ethanol to no alcohol taste.
8. a kind of preparation method of the anal fistula debridement Chinese medicine composition described in claim 3 or 4 is it is characterised in that the method includes
Following steps：
By climbing groundsel, dandelion, mother chrysanthemum and the mixing of Verbena officinalis 4 taste medicinal material, decocting boils 2～3 times, 1～2 hour every time, merges
Decocting liquid, filters, obtains extract, and extract is concentrated into medicinal material and extract weight ratio for 1：When 1～2, cooling, plus extract
The mass concentration of 2～3 times amount is more than 95% ethanol, precipitates more than 24 hours, filtration, reclaims ethanol to no alcohol taste, then plus
Borax mixes.
9. a kind of anal fistula debridement lotion is it is characterised in that this lotion is made up of the component of following weight portion：
Climbing groundsel 50～100 weight portion, dandelion 50～100 weight portion, mother chrysanthemum 40～60 weight portion, Verbena officinalis 40～60 weight
Amount part, glycerine 30～60 weight portion, then add pure water to 180g-320g crude drug/500-1000ml.
10. a kind of preparation method of the anal fistula debridement washing lotion described in claim 9 is it is characterised in that the method includes following step
Suddenly：
By climbing groundsel, dandelion, mother chrysanthemum and the mixing of Verbena officinalis 4 taste medicinal material, decocting boils 2～3 times, 1～2 hour every time, merges
Decocting liquid, filters, obtains extract, and extract is concentrated into medicinal material and extract weight ratio for 1：When 1～2, cooling, plus extract
The mass concentration of 2～3 times amount is more than 95% ethanol, precipitates more than 24 hours, filtration, reclaims ethanol to no alcohol taste, then plus
Glycerine and pure water mix to full dose.
A kind of 11. anal fistula debridement lotions are it is characterised in that this lotion is made up of the component of following weight portion：
Climbing groundsel 50～100 weight portion, dandelion 50～100 weight portion, mother chrysanthemum 40～60 weight portion, Verbena officinalis 40～60 weight
Amount part, borax 15～25 weight portion, glycerine 30～60 weight portion, then add pure water to 180g-320g crude drug/500-
1000ml.
The preparation method of the anal fistula debridement washing lotion described in a kind of 12. claims 11 is it is characterised in that the method includes following step
Suddenly：
By climbing groundsel, dandelion, mother chrysanthemum and the mixing of Verbena officinalis 4 taste medicinal material, decocting boils 2～3 times, 1～2 hour every time, merges
Decocting liquid, filters, obtains extract, and extract is concentrated into medicinal material and extract weight ratio for 1：When 1～2, cooling, plus extract
The mass concentration of 2～3 times amount is more than 95% ethanol, precipitates more than 24 hours, filtration, reclaims ethanol to no alcohol taste, then plus
Borax, glycerine and pure water mix to full dose.
Anal fistula debridement Chinese medicine composition described in 13. claim 1-4 any one claims is in the medicine of preparation anal fistula pressure sore
Application in thing.
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Cited By (1)
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| CN115957258A (en) * | 2022-12-06 | 2023-04-14 | 中国人民解放军东部战区总医院 | Wound healing lotion and preparation process and application thereof |
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