CN115427431A - 衍生自腺病毒五邻体基座的可变结构域的工程化多肽 - Google Patents

衍生自腺病毒五邻体基座的可变结构域的工程化多肽 Download PDF

Info

Publication number
CN115427431A
CN115427431A CN202180024156.1A CN202180024156A CN115427431A CN 115427431 A CN115427431 A CN 115427431A CN 202180024156 A CN202180024156 A CN 202180024156A CN 115427431 A CN115427431 A CN 115427431A
Authority
CN
China
Prior art keywords
group
amino acid
fragment
present
terminus
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202180024156.1A
Other languages
English (en)
Chinese (zh)
Inventor
弗雷德里克·加佐尼
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Amorflor Co ltd
Original Assignee
Amorflor Co ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amorflor Co ltd filed Critical Amorflor Co ltd
Publication of CN115427431A publication Critical patent/CN115427431A/zh
Pending legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1055—Protein x Protein interaction, e.g. two hybrid selection
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/10011—Adenoviridae
    • C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
    • C12N2710/10322—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/10011—Adenoviridae
    • C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
    • C12N2710/10323—Virus like particles [VLP]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/10011—Adenoviridae
    • C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
    • C12N2710/10333—Use of viral protein as therapeutic agent other than vaccine, e.g. apoptosis inducing or anti-inflammatory

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Virology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Microbiology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicinal Preparation (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
CN202180024156.1A 2020-02-05 2021-02-05 衍生自腺病毒五邻体基座的可变结构域的工程化多肽 Pending CN115427431A (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
EP20155742.8 2020-02-05
EP20155742 2020-02-05
EP20155982.0 2020-02-06
EP20155982 2020-02-06
PCT/EP2021/052884 WO2021156489A1 (en) 2020-02-05 2021-02-05 Engineered polypeptides derived from variable domain of adenovirus penton base

Publications (1)

Publication Number Publication Date
CN115427431A true CN115427431A (zh) 2022-12-02

Family

ID=74561908

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202180024156.1A Pending CN115427431A (zh) 2020-02-05 2021-02-05 衍生自腺病毒五邻体基座的可变结构域的工程化多肽

Country Status (10)

Country Link
US (1) US20230101439A1 (enExample)
EP (1) EP4100421A1 (enExample)
JP (1) JP2023513512A (enExample)
CN (1) CN115427431A (enExample)
AU (1) AU2021216124A1 (enExample)
BR (1) BR112022015461A2 (enExample)
CA (1) CA3166852A1 (enExample)
MX (1) MX2022009574A (enExample)
WO (1) WO2021156489A1 (enExample)
ZA (1) ZA202209759B (enExample)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2024042100A1 (en) 2022-08-22 2024-02-29 Imophoron Limited Adenovirus penton-based virus-like particles

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105112428A (zh) * 2009-02-02 2015-12-02 葛兰素史密斯克莱生物公司 猿腺病毒核酸和氨基酸序列，包含其的载体及其用途
CN109415739A (zh) * 2016-03-31 2019-03-01 欧洲分子生物学实验室 腺病毒外壳蛋白衍生递送载体
CN110300597A (zh) * 2016-12-09 2019-10-01 葛兰素史密丝克莱恩生物有限公司 腺病毒多核苷酸和多肽

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080004228A1 (en) 2004-03-09 2008-01-03 Imre Berger New Expression Tools for Multiprotein Applications
WO2010100278A2 (en) 2009-03-06 2010-09-10 Europäisches Laboratorium für Molekularbiologie (EMBL) Nucleic acids for cloning and expressing multiprotein complexes
PL222497B1 (pl) * 2009-06-26 2016-08-31 Inst Biochemii I Biofizyki Pan Wektorowa cząsteczka wirusopodobna, sposób jej wytwarzania, kompozycja farmaceutyczna oraz zastosowanie wektorowej cząsteczki wirusopodobnej
EP3655539A1 (en) * 2017-07-21 2020-05-27 GlaxoSmithKline Biologicals S.A. Chikungunya virus antigen constructs
EP3830250A1 (en) * 2018-07-31 2021-06-09 Imophoron Limited Multimerizing polypeptides derived from jelly roll fold domain of adenovirus penton base

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105112428A (zh) * 2009-02-02 2015-12-02 葛兰素史密斯克莱生物公司 猿腺病毒核酸和氨基酸序列，包含其的载体及其用途
CN109415739A (zh) * 2016-03-31 2019-03-01 欧洲分子生物学实验室 腺病毒外壳蛋白衍生递送载体
CN110300597A (zh) * 2016-12-09 2019-10-01 葛兰素史密丝克莱恩生物有限公司 腺病毒多核苷酸和多肽

Also Published As

Publication number Publication date
AU2021216124A1 (en) 2022-09-29
MX2022009574A (es) 2022-11-16
JP2023513512A (ja) 2023-03-31
CA3166852A1 (en) 2021-08-12
WO2021156489A1 (en) 2021-08-12
ZA202209759B (en) 2023-07-26
BR112022015461A2 (pt) 2022-10-11
EP4100421A1 (en) 2022-12-14
US20230101439A1 (en) 2023-03-30

Similar Documents

Publication Publication Date Title
CN1859923B (zh) Hpv 45 l1在酵母中的优化表达
US11274127B2 (en) Adenoviral coat protein derived delivery vehicles
Gao et al. Designing a soluble near full-length HIV-1 gp41 trimer
JP2021532836A (ja) アデノウイルス ペントンベースのゼリーロールフォールドドメイン由来である多量体化ポリペプチド
US20170326224A1 (en) Human papilloma virus constructs
CN104710515A (zh) 人乳头瘤病毒l1蛋白突变体及其制备方法
CN112279921B (zh) 用于胞内递送分子的复合物
Motevalli et al. Supercharged green fluorescent protein delivers HPV16E7 DNA and protein into mammalian cells in vitro and in vivo
CN114560915A (zh) 一种改造的高滴度SARS-CoV-2假病毒
WO2002004007A9 (en) Stable (fixed) forms of viral capsid proteins, fusion proteins and uses thereof
JP7062595B2 (ja) 複合ポリペプチド単量体、細胞浸透機能を有する当該複合ポリペプチドの単量体の会合体、及び、当該会合体を有効成分とする皮下、皮内、経皮又は筋肉内投与用ノロウイルスコンポーネントワクチン
CN105431457A (zh) 单结构域抗体展示
US20230101439A1 (en) Engineered Polypeptides Derived From Variable Domain of Adenovirus Penton Base
Kardani et al. B1 protein: a novel cell penetrating protein for in vitro and in vivo delivery of HIV-1 multi-epitope DNA constructs
US10620214B2 (en) Chloroalkane penetration method for quantifying access of a molecule into a cell
CN119841901A (zh) 一种抑制包涵体形成并促进Gag-Like蛋白纳米颗粒自组装的短肽组合物
WO2005077976A2 (en) Coiled-coil domains from c4b-binding protein
RU2820522C2 (ru) Мультимеризующиеся полипептиды, происходящие от домена с укладкой типа рулет основания пентона аденовируса
KR102201154B1 (ko) 폴리글루타메이트-TAT-Cre 융합 단백질의 제조방법
Kim et al. Engineering of Recombinant Human Papillomavirus 16 L1 Protein for Incorporation with para-Azido-L-Phenylalanine
CN119798408A (zh) 一种识别hpv多抗原表位的tcr及其编码序列
Sunstrom Specific DNA binding by polyomavirus large T antigen
HK1232549A1 (en) Tear lipocalin muteins binding il-4 r alpha

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination