CN115407074A - Automatic device and automatic detection method for air sampling and detection - Google Patents

Abstract

The invention discloses an automatic device and an automatic detection method for air sampling and detection, wherein the automatic device for air sampling and detection comprises a shell, an acquisition module, a pretreatment module, a manipulator, a liquid transfer module, a detection module and a recovery module. The air collection, pretreatment and detection are realized through the cooperation of different modules, manual treatment is not needed, and the technical scheme of the invention can improve the efficiency of environmental air detection.

Description

Automatic device and automatic detection method for air sampling and detection

Technical Field

The invention relates to the technical field of air detection, in particular to an automatic device and an automatic detection method for air sampling and detection.

Background

The components in the air are many, such as microorganisms, bioaerosols and large particulate matters, wherein the bioaerosols comprise biological particles such as fungi, bacteria and viruses, the large particulate matters comprise particles such as PM2.5 and PM1.0, and the substances can influence the health condition of a human body, and an improvement scheme can be provided in time by accurately judging the surrounding environment.

In the prior art, there is a device for rapid detection, and the existing device can only directly collect through a swab or a sensor and detect through a small independent detection box, and the detection quantity is small, and the detection precision is not high. Moreover, the swab has limited acquisition capabilities and cannot accurately represent the specific circumstances of the environment in which the object to be detected is located.

Disclosure of Invention

The invention mainly aims to provide an automatic device for sampling and detecting air, which aims to realize automatic integrated equipment for batch operations of air collection, pretreatment, detection and the like, and simultaneously continuously collects and detects multiple samples, so that the precision of ambient air detection is improved, and the detection efficiency is also improved.

In order to achieve the above object, the present invention provides an automatic device for air sampling and detection, comprising:

the device comprises a shell, a first support, a second support and a third support, wherein the shell is provided with a working chamber, the bottom of the working chamber is provided with a mounting support, and the top of the working chamber is provided with a movable support;

the pretreatment module is arranged in the working chamber and is arranged on the mounting bracket, the pretreatment module comprises a storage device and a pretreatment device, the storage device at least stores a first reagent, a second reagent, a third reagent and a sampling container, the mounting bracket is provided with a pretreatment position, and the pretreatment device is arranged on the pretreatment position;

the collection module is arranged in the working chamber and is arranged on the mounting bracket, the collection module is used for collecting air and collecting objects to be detected in the air into the sampling container, the collection module is provided with a collection position, the shell is exposed with a collection port, the collection port is used for collecting the objects to be detected in the air, and the pretreatment module can enable the sampling container to move back and forth between the collection position and the pretreatment position;

the mechanical arm is arranged in the working cavity and is arranged on the movable support, the sampling container is provided with a first cover body, and the mechanical arm is at least used for moving the sampling container to the pretreatment device and screwing or unscrewing the first cover body and the sampling container;

the pipetting module is arranged in the working chamber and is arranged on the moving bracket, and the pipetting module is used for moving the first reagent, the second reagent or the third reagent;

the detection module is arranged in the working chamber and is arranged on the mounting bracket, and the detection module is used for detecting the object to be detected; and

the recovery module is arranged in the working chamber and is installed on the mounting bracket, and the recovery module is used for recovering the used first reagent, the second reagent, the third reagent and the sampling container.

Optionally, the pretreatment device comprises a container support, a container moving mechanism and a container lifting mechanism; the container support is arranged on the container moving mechanism, one end of the container moving mechanism is arranged at the pretreatment position, the other end of the container moving mechanism is arranged at the collection position, and the container moving mechanism can drive the container support to move to the pretreatment position or the collection position; the container lifting mechanism is arranged at the collecting position, and when the container support moves to the collecting position, the container lifting mechanism 313 jacks the container support so that the collecting container in the container support can ascend to the collecting module.

Optionally, the storage device comprises a first storage area storing a plurality of TIP headers; a second storage area in which a sampling container to which the first reagent is added in advance is stored; a third storage area in which the second reagent and the third reagent are stored.

Optionally, the recovery module comprises a first waste material box, a second waste material box and a third waste material box which are arranged on the mounting bracket; the first waste box is arranged in the first storage area and used for recovering the used TIP head; the second waste box is arranged in the second storage area and used for recycling the used sampling container; and the third waste material box is arranged close to the detection module and used for recovering the detected reagent bottle.

Optionally, the third waste bin is provided with an inversion means; the reversing device comprises a reversing bracket and a reversing motor, the reversing bracket is used for accommodating the reagent bottle, and the reversing motor drives the reversing bracket to rotate at least 90 degrees towards the third waste material box; the second waste material box is provided with a waste material opening, and the waste material opening is provided with a guide inclined plane extending towards the inside of the second waste material box.

Optionally, the detection module includes an air detection mechanism and an automatic cover-turning device, the air detection mechanism is provided with an accommodating groove, and the automatic cover-turning structure is rotatably covered on the accommodating groove, so that a sealed detection space is formed in the accommodating groove; the air detection mechanism can detect the object to be detected collected from the air by a fluorescent labeling method.

Optionally, the collection module is an air sampling device, and the air sampling device includes a fan box, an air guide pipe and a centrifugal part; the fan box is communicated with the centrifugal part through the air guide pipe, an air inlet is formed in the side part of the centrifugal part and communicated with the collection port, a collection hole is formed in the bottom of the centrifugal part and arranged downwards, and an opening of the sampling container can be abutted to the collection hole.

Optionally, the automation device for air sampling and detection further comprises a protection cover device, the protection cover device comprises a protection cover body and a cover body motor, and the cover body motor can rotationally drive the protection cover body to cover the acquisition port.

Optionally, the moving bracket comprises a horizontal moving mechanism and a mounting member connected to the horizontal moving mechanism; the mounting part is provided with a first horizontal moving mechanism and a second horizontal moving mechanism, the liquid transfer module is arranged on the first horizontal moving mechanism, and the manipulator is arranged on the second horizontal moving mechanism.

The invention also provides an automatic detection method, which comprises the automatic air sampling and detection device, wherein the automatic air sampling and detection device at least comprises a second reagent bottle and a third reagent bottle, the second reagent bottle stores a second reagent and is provided with a second cover body for covering the second reagent bottle, and the third reagent bottle stores a third reagent and is provided with a third cover body for covering the third reagent bottle;

the automatic device for sampling and detecting air adopts the automatic detection method, and the automatic detection method comprises the following steps:

the manipulator moves to the storage device;

the sampling container is added with the first reagent in advance, and the mechanical arm grabs the sampling container in the storage device and moves to the pretreatment position;

the pretreatment device fixes the sampling container, and the mechanical arm unscrews and takes down the first cover body from the sampling container;

the pre-processing device moves the sampling container from the pre-processing position to the collection position;

the collection module sucks air from the collection port and collects the objects to be detected in the air into the sampling container;

the pretreatment device moves the sampling container with the object to be detected to the pretreatment position;

the manipulator grabs a second reagent bottle in the storage device and moves to the pretreatment position; the second reagent bottle is fixed by the pretreatment device, and the second cover body is unscrewed and taken down from the second reagent bottle by the manipulator;

the manipulator grabs a third reagent bottle in the storage device and moves to the pretreatment position; the third reagent bottle is fixed by the pretreatment device, and the third cover body is unscrewed and taken down from the third reagent bottle by the manipulator;

the pipetting module moves the first reagent and the object to be detected in the sampling container to the second reagent in the second reagent bottle and uniformly mixes the first reagent and the object to be detected; the liquid transferring module moves the first reagent, the second reagent and the object to be detected which are uniformly mixed in the second reagent bottle to a third reagent in a third reagent bottle, so that final liquid to be detected is obtained;

the mechanical arm covers the first cover body on the sampling container, the mechanical arm covers the second cover body on the second reagent bottle, the mechanical arm covers the third cover body on the third reagent bottle, and the third reagent bottle is moved to the detection module for detection;

the manipulator moves the second reagent bottle and the sampling container in the pretreatment position and the third reagent bottle in the detection module to the recovery module.

According to the technical scheme, the collection module is used for automatically collecting air and collecting the object to be detected in the air so as to be used for processing and detecting a subsequent module, and the collected object to be processed is transferred to a preprocessing position and added with a reagent through the preprocessing module, the manipulator and the liquid transfer module. The manipulator can move the pretreated reagent bottle where the object to be detected is located to the detection module for detection and obtain a result. In order to meet the requirement of higher automation, the automation device for air sampling and detection is further provided with a recovery module, the recovery module is arranged in the working chamber and fixedly arranged on the mounting bracket, and the recovery module can recover the first reagent, the second reagent, the third reagent and the sampling container, so that the next round of sampling, pretreatment and detection can be smoothly carried out. It should be noted that the device of this embodiment integrates a plurality of steps such as collection, preliminary treatment and detection, and detects a plurality of samples simultaneously, in succession, has high-efficient detection efficiency simultaneously to form the incessant detection to ambient air, and then makes the testing result more accurate.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the structures shown in the drawings without creative efforts.

FIG. 1 is a schematic structural diagram of an automated air sampling and detection apparatus according to an embodiment of the present invention;

FIG. 2 is a schematic diagram of an internal structure of an automatic air sampling and detecting device according to an embodiment of the present invention;

FIG. 3 is a schematic structural diagram of one embodiment of a manipulator and pipetting module of the automated device for air sampling and detection according to the present invention;

FIG. 4 is a schematic structural diagram of an embodiment of a pre-processing module, an acquisition module, a detection module and a recovery module of the automated device for air sampling and detection according to the present invention;

FIG. 5 is a schematic diagram of a pre-processing module of the automated air sampling and testing apparatus according to the present invention;

FIG. 6 is a schematic structural view of a container lifting mechanism of a pre-processing module of the automated air sampling and testing device of the present invention;

FIG. 7 is a schematic view of the mechanism of the container support of the automated device for air sampling and testing according to the present invention;

FIG. 8 is a schematic view of the mechanism of the processing table of the automated device for air sampling and detection according to the present invention;

FIG. 9 is a schematic view of the mechanism of the protective cover device of the automatic air sampling and detecting device of the present invention;

FIG. 10 is a schematic structural view of a second waste material box and a third waste material box of the automatic device for air sampling and detection according to the present invention;

FIG. 11 is a schematic view of the inversion device of the automated air sampling and testing apparatus of the present invention;

FIG. 12 is a schematic view of the detection module of the automated air sampling and detection apparatus of the present invention;

fig. 13 is a schematic structural diagram of an automatic cover-turning device of the automatic device for air sampling and detection according to the present invention.

The reference numbers indicate:

the implementation, functional features and advantages of the present invention will be further described with reference to the accompanying drawings.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

It should be noted that all directional indicators (such as up, down, left, right, front, and back \8230;) in the embodiments of the present invention are only used to explain the relative positional relationship between the components, the motion situation, etc. in a specific posture (as shown in the attached drawings), and if the specific posture is changed, the directional indicators are changed accordingly.

In the present invention, unless otherwise expressly stated or limited, the terms "connected," "secured," and the like are to be construed broadly, and for example, "secured" may be a fixed connection, a removable connection, or an integral part; can be mechanically or electrically connected; they may be directly connected or indirectly connected through intervening media, or they may be connected internally or in any other suitable relationship, unless expressly stated otherwise. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.

In addition, if there is a description of "first", "second", etc. in an embodiment of the present invention, the description of "first", "second", etc. is for descriptive purposes only and is not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one of the feature. In addition, the meaning of "and/or" appearing throughout includes three juxtapositions, exemplified by "A and/or B" including either A or B or both A and B. In addition, technical solutions between various embodiments may be combined with each other, but must be realized by a person skilled in the art, and when the technical solutions are contradictory or cannot be realized, such a combination should not be considered to exist, and is not within the protection scope of the present invention.

The invention provides an automatic device for air sampling and detection.

Referring to fig. 1 to 13, in an embodiment of the present invention, the automatic air sampling and detecting device includes:

the device comprises a shell 100, a base, a movable support 2 and a mounting support 1, wherein the shell is provided with a working chamber, and the bottom of the working chamber is provided with the mounting support 1;

the pretreatment module 3 is arranged in the working chamber and is mounted on the mounting bracket 1, the pretreatment module 3 comprises a storage device 32 and a pretreatment device 31, the storage device 32 at least stores a first reagent, a second reagent, a third reagent and a sampling container 321, the mounting bracket 1 is provided with a pretreatment position, and the pretreatment device 31 is arranged on the pretreatment position;

the collection module is arranged in the working chamber and is mounted on the mounting bracket 1, the collection module is used for collecting air and collecting objects to be detected in the air into the sampling container 321, the collection module is provided with a collection position, the shell 100 is exposed with a collection port 45, the collection port 45 is used for collecting the objects to be detected in the air, and the pretreatment module 3 can move the sampling container 321 between the collection position and the pretreatment position;

the mechanical arm 5 is arranged in the working chamber and is mounted on the movable support 2, the sampling container 321 is provided with a first cover body, and the mechanical arm 5 is at least used for moving the sampling container 321 to the pretreatment device 31 and screwing or unscrewing the first cover body and the sampling container 321;

the pipetting module 6 is arranged in the working chamber and is installed on the moving bracket 2, and the pipetting module 6 is used for moving the first reagent, the second reagent or the third reagent;

the detection module 7 is arranged in the working chamber and is installed on the installation support 1, and the detection module 7 is used for detecting the object to be detected; and

and the recovery module 8 is arranged in the working chamber and is installed on the mounting bracket 1, and the recovery module 8 is used for recovering the used first reagent, the second reagent, the third reagent and the sampling container 321.

According to the technical scheme, the collection module is used for automatically collecting air and collecting the objects to be detected in the air for the subsequent module to process and detect, and the pretreatment module 3, the manipulator 5 and the liquid-transferring module 6 are used for transferring the collected objects to be processed to a pretreatment position, adding a reagent and the like. The manipulator 5 can move the pretreated reagent bottle containing the object to be detected to the detection module 7 for detection and obtain a result. In order to satisfy higher automation demand, the automation equipment of air sampling and detection still is provided with recovery module 8, recovery module 8 locates work chamber, fixed mounting in installing support 1, recovery module 8 is recoverable first reagent, second reagent, third reagent and sampling container 321 to the operation of next round sampling, preliminary treatment and detection goes on smoothly. It should be noted that the device of this embodiment integrates a plurality of steps such as collection, preliminary treatment and detection, and detects a plurality of samples simultaneously, in succession, has high-efficient detection efficiency simultaneously to form the incessant detection to ambient air, and then makes the testing result more accurate.

The automatic air sampling and detecting device comprises a plurality of modules which are matched to realize the processes of collecting, processing and detecting microorganisms or biological aerosol or atmospheric particulate matters in the air.

What is introduced first is the collection module in the automatic device for air sampling and detection, which is disposed in the working chamber and is fixedly mounted to the mounting bracket 1, and the collection module can be used to collect air in the surrounding environment and collect substances in the air.

In this embodiment, the housing 100 is exposed with the collection port 45, the collection module has a collection position, the collection port 45 is used for collecting the object to be detected in the air, the collection position is provided with the sampling container 321, and the air processed by the collection module can collect the object to be detected in the air in the sampling container 321.

Specifically, the collection module is an air sampling device 4, the air sampling device 4 includes a fan box 41, an air guide duct 42 and a centrifugal portion 43, an air inlet 44 is formed in a side portion of the centrifugal portion 43, the fan in the fan box 41 provides suction force, so that air enters the air inlet 44 of the centrifugal portion 43 from a collection port 45, and microorganisms, bioaerosols or atmospheric particulates in the air enter the sampling container 321 from a collection hole 47 formed in the bottom of the centrifugal portion 43 under the centrifugal action of the centrifugal portion 43, it should be noted that the collection hole 47 is formed in the bottom of the centrifugal portion 43 and faces downward, and the sampling container 321 abuts against the collection hole 47.

Further, in this embodiment, the automatic apparatus for air sampling and detecting further includes a protection cover device 46, the protection cover device 46 includes a protection cover 461 and a cover motor 462, and the cover motor 462 can rotatably drive the protection cover 461 to cover the collecting port 45. Because the automation device for air sampling and detection in this embodiment avoids the continuous interference of the external environment on the automation device for air sampling and detection after completing the collection action, the collection port 45 is provided with the protection cover device 46, and the protection cover 461 is rotationally driven by the cover motor 462 to cover the collection port 45, thereby preventing other air from entering the collection module and affecting the accuracy of detection.

What should be introduced is the pretreatment module 3, and the object to be detected in the middle sampling container 321 cannot be directly detected, but can be detected in the detection module 7 after a certain process, adding a specific reagent and processing.

In the present embodiment, the preprocessing module 3 includes a storage device 32 and a preprocessing device 31.

The storage device 32 stores at least three reagents, which are named as a first reagent, a second reagent and a third reagent, respectively, since the three reagents are used in the present embodiment. However, in this example, the three reagents are all reagents required for ATP fluorescence detection.

Specifically, the storage device 32 is fixedly arranged on the mounting bracket 1, the storage device 32 includes a first storage area 321, and the first storage area 321 stores a plurality of TIP heads for the pipetting module 6 to replace the TIP heads, so that the whole apparatus can prevent contamination of reagents during automatic pipetting, thereby affecting the detection result. The storage device 32 further includes a second storage area 322, and the second storage area 322 stores a sampling container 321, wherein the first reagent is pre-added into the sampling container 321. The storage device 32 further includes a third storage area 323, the third storage area 323 stores a second reagent and a third reagent, wherein the second reagent is contained in a second reagent bottle, the second reagent bottle is provided with a second cover, the third reagent is contained in a third reagent bottle, and the third reagent bottle is provided with a third cover. The setting of lid can effectually prevent the volatilization and the diffusion of reagent.

The pretreatment device 31 includes a container moving mechanism 312, a container lifting mechanism 313, a container holder 311, and a treatment table 314.

The container support 311 is disposed on the container moving mechanism 312, one end of the container moving mechanism 312 is disposed on the pretreatment position, and the other end of the container moving mechanism 312 is disposed on the collection position, and the container moving mechanism 312 can drive the container support 311 to move to the pretreatment position or the collection position. The container lifting mechanism 313 is disposed at the collection position, and when the container support 311 moves to the collection position, the container lifting mechanism 313 lifts the container support 311, so that the sampling container 321 in the container support 311 rises to the collection module. The processing station 314 is set to the pre-processing position. The container holder 311 is disposed on the container moving mechanism 312, and the container holder 311 is disposed with a receiving groove for receiving the sampling container 321.

Specifically, the processing platform 314 is provided with a first clamping portion 3141, the processing platform 314 is provided with a first sliding groove, and the first clamping portion 3141 is slidably arranged on the first sliding groove; the container holder 311 is further provided with a second clamping portion 3116 and a second slide groove, the second clamping portion 3116 being slidably provided in the second slide groove. The first clamping portion 3141 and the second clamping portion 3116 are separated from each other, the container moving mechanism 312 can drive the container holder 311 to abut against the processing table 314, the first clamping portion 3141 abuts against the second clamping portion 3116, and the first clamping portion 3141 moves towards the first chute direction to clamp the reagent bottle; the second clamping portion 3116 moves in the direction of the second chute to clamp the sampling vessel 321. The second clamping portion 3116 clamps the sampling container 321 while the first clamping portion 3141 clamps the reagent bottle, so that the manipulator 5 having the rotatable clamping jaws can tighten or loosen the cover bodies of the second reagent bottle, the third reagent bottle, and the sampling container 321 in the pretreatment position.

The container moving mechanism 312 is a screw mechanism to drive the container holder 311 disposed on the screw mechanism to reciprocate between the pretreatment position and the collection position. It should be noted that the collection position is also a jacking position, the container lifting mechanism 313 is disposed near the jacking position, the container lifting mechanism 313 is provided with another set of screw rod mechanisms, the container lifting mechanism 313 extends towards the jacking position and is provided with an abutting portion, the abutting portion can abut against the container support 311 to move upwards along the height direction, and then the opening of the sampling container 321 in the container support 311 abuts against the collection hole 47 of the eccentric portion 43.

Furthermore, a pipetting module 6 is arranged in the working chamber and is mounted on the movable support 2, and the pipetting module 6 is used for moving the first reagent, the second reagent and the third reagent. It should be noted that the pipetting module 6 has a pipette TIP that can mount a TIP head in the first storage area 321, and the pipetting module 6 is further provided with a push sleeve that can move downward in the direction of the pipette TIP, so that the TIP head provided on the pipette TIP drops and a new TIP can be mounted on the pipette TIP.

The manipulator 5 is arranged in the working chamber and is arranged on the movable support 2, the sampling container 321 is provided with a first cover body, the manipulator 5 comprises a rotatable clamping jaw, and the clamping jaw clamps the first cover body and then rotates to realize screwing or unscrewing of the first cover body.

It should be noted that the moving bracket 2 is a planar moving mechanism, and the moving bracket 2 includes two sets of screw rod mechanisms, and the moving directions of the two sets of screw rod mechanisms are perpendicular to each other. And in order to make the space compacter, lead screw and motor are through belt pulley connection, have reduced the occupied length of lead screw mechanism in the space. Specifically, the moving bracket 2 comprises a horizontal moving mechanism and a mounting part connected with the horizontal moving mechanism; the mounting piece is provided with a first horizontal moving mechanism 21 and a second horizontal moving mechanism 22, the pipetting module 6 is arranged on the first horizontal moving mechanism 21, and the manipulator 5 is arranged on the second horizontal moving mechanism 22.

Further, the recovery module 8 is used for recovering a reagent bottle, a reagent, a TIP and a sampling container 321, and the recovery module 8 comprises a first waste material box 81, a second waste material box 82 and a third waste material box 83 which are arranged on the mounting bracket 1; the first waste box 81 is disposed in the first storage area 321, and is used for recovering the used TIP heads; the second waste material box 82 is disposed in the second storage area 322, and is used for recovering the used sampling container 321; the third waste material box 83 is arranged close to the detection module 7 and used for recovering the detected reagent bottles.

In one embodiment, the third waste material box 83 is provided with an inverting device 84; the reversing device 84 comprises a reversing frame 841 for accommodating the reagent bottles, and a reversing motor 842 for driving the reversing frame 841 to rotate at least 90 degrees in the direction of the third waste material box 83; the second waste material box 82 is provided with a waste material port 85, and the waste material port 85 is provided with a guide inclined surface 86 extending towards the inside of the second waste material box 82.

As for the detection module 7, in this embodiment, the detection module 7 includes an air detection mechanism 71 and an automatic lid-turning device 72, the air detection mechanism 71 is provided with an accommodating groove 73, and the automatic lid-turning structure is rotatably covered on the accommodating groove 73, so that a sealed detection space is formed in the accommodating groove 73; the air detecting mechanism 71 can detect the object to be detected collected from the air by a fluorescent labeling method. Specifically, in this embodiment, the air detection mechanism 71 includes a photoelectric detection module 7 and a sample accommodating structure, the photoelectric detection module 7 includes an electric control board, an excitation light source and a photomultiplier tube, and the electric control board is electrically connected to the photomultiplier tube. The excitation light source irradiates to the sample accommodating structure, and the photoelectric detection module 7 collects the background value of the object to be detected in the sample accommodating structure, so that the object to be detected is detected.

The automatic flip device 72 includes a cover 721, a flip motor 722, a return spring, and a cover rod 723. The flip motor 722 drives the cover rod 723 to rotate, so as to push the cover 721 to cover the air detection mechanism 71. While the return of the cover 721 is achieved by a return spring.

The present invention further provides an automatic detection method, which includes the air sampling and detecting automation device, and the specific structure of the air sampling and detecting automation device refers to the above embodiments.

The automatic air sampling and detecting device at least comprises a second reagent bottle and a third reagent bottle, wherein the second reagent bottle stores the second reagent and is provided with a second cover body for covering the second reagent bottle, and the third reagent bottle stores the third reagent and is provided with a third cover body for covering the third reagent bottle;

the automatic air sampling and detecting device adopts the automatic detecting method, and the automatic detecting method comprises the following steps:

the robot 5 moves to the storage device 32;

the first reagent is added in advance to the sampling container 321, and the manipulator 5 grasps the sampling container 321 in the storage device 32 and moves to the pretreatment position;

the pretreatment device 31 fixes the sampling container 321, and the manipulator 5 unscrews and removes the first cover from the sampling container 321;

the pre-processing means 31 moves the sampling container 321 from the pre-processing position to the collection position;

the collection module sucks air from the collection port 45 and collects the objects to be detected in the air into the sampling container 321;

the pretreatment device 31 moves the sampling container 321 with the object to be detected collected to the pretreatment position;

the manipulator 5 grabs the second reagent bottle in the storage device 32 and moves to the pretreatment position; the pretreatment device 31 fixes the second reagent bottle, and the manipulator 5 unscrews and takes off the second cover from the second reagent bottle;

the manipulator 5 grabs a third reagent bottle in the storage device 32 and moves to the pretreatment position; the pretreatment device 31 fixes the third reagent bottle, and the manipulator 5 unscrews and removes the third cover from the third reagent bottle;

the pipetting module 6 moves the first reagent and the object to be detected in the sampling container 321 to the second reagent in the second reagent bottle, and uniformly mixes the first reagent and the object to be detected; the liquid transferring module 6 moves the first reagent, the second reagent and the object to be detected, which are uniformly mixed in the second reagent bottle, into a third reagent in a third reagent bottle, so as to obtain a final liquid to be detected;

the manipulator 5 covers the first cover body on the sampling container 321, the manipulator 5 covers the second cover body on the second reagent bottle, the manipulator 5 covers the third cover body on the third reagent bottle, and the third reagent bottle is moved to the detection module 7 for detection;

the robot 5 moves the second reagent bottle and the sampling container 321 in the pre-processing position and the third reagent bottle in the detection module 7 to the recovery module 8.

The above description is only an alternative embodiment of the present invention, and not intended to limit the scope of the present invention, and all modifications and equivalents of the present invention, which are made by the contents of the present specification and the accompanying drawings, or directly/indirectly applied to other related technical fields, are included in the scope of the present invention.

Claims (10)

1. An automation device for air sampling and detection, comprising:

the device comprises a shell, a first support, a second support and a third support, wherein the shell is provided with a working chamber, the bottom of the working chamber is provided with a mounting support, and the top of the working chamber is provided with a movable support;

the pretreatment module is arranged in the working chamber and is installed on the installation support, the pretreatment module comprises a storage device and a pretreatment device, the storage device at least stores a first reagent, a second reagent, a third reagent and a sampling container, the installation support is provided with a pretreatment position, and the pretreatment device is arranged on the pretreatment position;

the collection module is arranged in the working chamber and is arranged on the mounting bracket, the collection module is used for collecting air and collecting an object to be detected in the air into the sampling container, the collection module is provided with a collection position, a collection port is exposed on the shell and is used for collecting the object to be detected in the air, and the pretreatment module can enable the sampling container to move back and forth between the collection position and the pretreatment position;

the mechanical arm is arranged in the working chamber and is arranged on the movable support, the sampling container is provided with a first cover body, and the mechanical arm is at least used for moving the sampling container to the pretreatment device and screwing or unscrewing the first cover body and the sampling container;

the pipetting module is arranged in the working chamber and is arranged on the moving bracket, and the pipetting module is used for moving the first reagent, the second reagent or the third reagent;

the detection module is arranged in the working chamber and is arranged on the mounting bracket, and the detection module is used for detecting the object to be detected; and

the recovery module is arranged in the working chamber and is installed on the mounting bracket, and the recovery module is used for recovering the used first reagent, the second reagent, the third reagent and the sampling container.

2. The automated air sampling and detection device of claim 1, wherein the pre-processing device comprises a container support, a container moving mechanism, and a container lifting mechanism;

the container support is arranged on the container moving mechanism, one end of the container moving mechanism is arranged at the pretreatment position, the other end of the container moving mechanism is arranged at the collection position, and the container moving mechanism can drive the container support to move to the pretreatment position or the collection position;

the container lifting mechanism is arranged at the collecting position, and when the container support moves to the collecting position, the container lifting mechanism 313 jacks the container support so that the collecting container in the container support can ascend to the collecting module.

3. The automated air sampling and detection apparatus of claim 1, wherein the storage device comprises a first storage area storing a plurality of TIP headers;

a second storage area in which a sampling container to which the first reagent is added in advance is stored;

a third storage area in which the second reagent and the third reagent are stored.

4. The automated air sampling and detection apparatus of claim 3, wherein the recycling module comprises a first waste material box, a second waste material box and a third waste material box disposed on the mounting bracket;

the first waste box is arranged in the first storage area and used for recovering the used TIP head;

the second waste box is arranged in the second storage area and used for recycling the used sampling container;

and the third waste material box is arranged close to the detection module and used for recovering the detected reagent bottle.

5. The automated air sampling and testing device of claim 4, wherein the third waste cartridge is provided with an inverting device;

the reversing device comprises a reversing bracket and a reversing motor, the reversing bracket is used for accommodating the reagent bottle, and the reversing motor drives the reversing bracket to rotate at least 90 degrees towards the third waste material box;

the second waste material box has seted up the waste material mouth, the waste material mouth is equipped with towards the guide inclined plane of second waste material box inside extension.

6. The automated air sampling and testing apparatus of claim 1, wherein the testing module comprises an air testing mechanism and an automatic cover-turning mechanism, the air testing mechanism is provided with a receiving cavity, and the automatic cover-turning mechanism is rotatably covered on the receiving cavity so that a sealed testing space is formed in the receiving cavity; the air detection mechanism can detect the object to be detected collected from the air by a fluorescent labeling method.

7. The automated air sampling and testing device of claim 1, wherein the collection module is an air sampling device comprising a fan box, a wind guide tube, and a centrifuge;

the fan box is communicated with the centrifugal part through the air guide pipe, an air inlet is formed in the side part of the centrifugal part and communicated with the collection port, a collection hole is formed in the bottom of the centrifugal part and arranged downwards, and an opening of the sampling container can abut against the collection hole.

8. The automated air sampling and testing device of claim 7, further comprising a protective cover device, wherein the protective cover device comprises a protective cover and a cover motor, and the cover motor rotatably drives the protective cover to cover the collection opening.

9. The automated air sampling and testing device of any one of claims 1 to 8, wherein the mobile carriage comprises a horizontal movement mechanism and a mounting member coupled to the horizontal movement mechanism;

the mounting part is provided with a first horizontal moving mechanism and a second horizontal moving mechanism, the liquid transfer module is arranged on the first horizontal moving mechanism, and the manipulator is arranged on the second horizontal moving mechanism.

10. An automatic testing method, comprising the automatic air sampling and testing device according to any one of claims 1 to 9, further comprising at least a second reagent bottle storing the second reagent and having a second lid body for closing the second reagent bottle, and a third reagent bottle storing the third reagent and having a third lid body for closing the third reagent bottle;

the automatic device for sampling and detecting air adopts the automatic detection method, and the automatic detection method comprises the following steps:

the manipulator moves to the storage device;

the sampling container is added with the first reagent in advance, and the mechanical arm grabs the sampling container in the storage device and moves to the pretreatment position;

the pretreatment device fixes the sampling container, and the mechanical arm unscrews and takes down the first cover body from the sampling container;

the pre-processing means moving the sampling container from the pre-processing position to the collection position;

the collection module sucks air from the collection port and collects the objects to be detected in the air into the sampling container;

the pretreatment device moves the sampling container with the object to be detected to the pretreatment position;

the manipulator grabs a second reagent bottle in the storage device and moves to the pretreatment position; the pretreatment device fixes the second reagent bottle, and the manipulator unscrews and takes down the second cover body from the second reagent bottle;

the manipulator grabs a third reagent bottle in the storage device and moves to the pretreatment position; the third reagent bottle is fixed by the pretreatment device, and the third cover body is unscrewed from the third reagent bottle and taken down by the manipulator;

the pipetting module moves the first reagent and the object to be detected in the sampling container to the second reagent in the second reagent bottle and mixes the first reagent and the object to be detected uniformly; the liquid transferring module moves the first reagent, the second reagent and the object to be detected which are uniformly mixed in the second reagent bottle to a third reagent in a third reagent bottle, so that final liquid to be detected is obtained;

the mechanical arm covers the first cover body on the sampling container, the mechanical arm covers the second cover body on the second reagent bottle, the mechanical arm covers the third cover body on the third reagent bottle, and the third reagent bottle is moved to the detection module for detection;

the manipulator moves the second reagent bottle and the sampling container in the pretreatment position and the third reagent bottle in the detection module to the recovery module.

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