CN115386455A - Microorganism detection and analysis system - Google Patents
Microorganism detection and analysis system Download PDFInfo
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- CN115386455A CN115386455A CN202211187104.9A CN202211187104A CN115386455A CN 115386455 A CN115386455 A CN 115386455A CN 202211187104 A CN202211187104 A CN 202211187104A CN 115386455 A CN115386455 A CN 115386455A
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/04—Flat or tray type, drawers
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/52—Mobile; Means for transporting the apparatus
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/46—Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/48—Automatic or computerized control
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Abstract
The invention belongs to the technical field of microbial detection and discloses an intelligent microbial detection analysis system.A camera shooting unit for shooting a culture dish is arranged in a culture room; the artificial intelligence module is used for processing the image acquired by the camera unit to obtain the number of bacterial colonies and the morphology of the bacterial colonies, and obtaining the species of the bacterial colonies according to the morphology of the bacterial colonies; the code scanning unit is arranged at an inlet of the culture chamber and used for identifying the two-dimensional code on the culture dish; the processing module is respectively with the unit of making a video recording, artificial intelligence module and sweep a yard unit electric connection, and the processing module is according to sweeping the two-dimensional code allocation that a yard unit discerned and corresponding the position of placing that the culture dish corresponds. The microorganism detection and analysis system provided by the invention is a whole system of inoculation, culture, detection and analysis, realizes automatic taking and placing of a culture dish through mechanical action, automatically takes pictures and records after culture, processes the pictures through an artificial intelligence module, and finally automatically generates an analysis report, and has high intelligent degree and convenient use.
Description
Technical Field
The invention belongs to the technical field of microbial detection, and particularly relates to a microbial detection analysis system.
Background
Microorganism culture refers to rapid growth and propagation of some microorganisms by means of artificially prepared culture media and artificially created culture conditions, and then the types and the number of the microorganisms growing on the culture media are obtained by photographing for subsequent analysis. Even some equipment can realize getting of culture dish and taking and shooing to the culture dish through apparatus and equipment at present, but all leave human cooperation, not only getting of culture dish is all artifical the completion, and subsequent analysis still need be through artifical the realization.
Disclosure of Invention
The present invention aims to solve the above technical problem at least to some extent. Therefore, the invention aims to provide a microorganism detection and analysis system.
The technical scheme adopted by the invention is as follows:
a microbiological detection analysis system comprising: the device comprises a culture chamber, a tray and a camera shooting unit, wherein the culture chamber is internally provided with a culture dish and the camera shooting unit is used for shooting the culture dish; the artificial intelligence module is used for processing the images acquired by the camera shooting unit to obtain the number of bacterial colonies and the morphology of the bacterial colonies, and obtaining the species of the bacterial colonies according to the morphology of the bacterial colonies; the code scanning unit is arranged at an inlet of the culture chamber and used for identifying the two-dimensional code on the culture dish; the processing module is respectively electrically connected with the camera shooting unit, the artificial intelligence module and the code scanning unit, and the processing module allocates the two-dimensional codes identified by the code scanning unit to the corresponding placing positions of the corresponding culture dishes.
Preferably, the microorganism detection and analysis system further comprises a report generation module electrically connected with the processing module, wherein the report generation module is used for calling the report template and filling the information obtained by processing of the artificial intelligence module and the information obtained by recognition of the code scanning unit into the report template to obtain an analysis report.
Preferably, a lifting assembly is arranged in the culture chamber, a manipulator for grabbing the tray is arranged on the lifting assembly, tray frames are arranged on two opposite sides of the lifting assembly, the tray is placed on the tray frames from top to bottom, and a plurality of placing openings are formed in each tray; the bottom of tray frame is located to the unit of making a video recording.
Preferably, lifting unit includes two vertical and parallel arrangement's sharp slip tables, and the slider of two sharp slip tables all links to each other with the elevating platform, and the manipulator is located on the elevating platform.
Preferably, the manipulator comprises a linear sliding table, and two adsorption units for adsorbing and releasing the tray are arranged on a sliding block of the linear sliding table in a back-to-back manner.
Preferably, the robot comprises a turntable, and an adsorption unit for adsorbing and releasing the tray is arranged on the turntable.
Preferably, be equipped with the transportation subassembly in the culture room, transport the subassembly and be located the lifting unit top.
Preferably, the microorganism detection and analysis system further comprises: the inoculation room is provided with isolation gloves, a printer is arranged in the inoculation room and used for printing a two-dimensional code, and the two-dimensional code comprises a unique code and inoculation information of each culture dish; transition storehouse, the both ends in transition storehouse link to each other with inoculation room and cultivation room respectively, are equipped with in the transition storehouse and transport the subassembly.
Preferably, the transfer assembly comprises a linear sliding table, a rotary table is arranged on a sliding block of the linear sliding table, and a clamping unit used for grabbing and loosening the culture dish is arranged on the rotary table.
Preferably, the inoculation information comprises inoculation time, inoculation personnel information and inoculation material information.
The invention has the beneficial effects that:
the microorganism detection and analysis system provided by the invention has the advantages that the automatic taking and placing of the culture dish are realized through the mechanical action under automatic control, the photographing record is automatically carried out after the culture, the photos are processed through the artificial intelligence module to obtain the number and the morphology of the bacterial colonies, the bacterial colony types are obtained according to the morphology of the bacterial colonies, and finally, the analysis report is automatically generated, so that the intelligent degree is high, and the use is convenient.
Drawings
FIG. 1 is a schematic block diagram of a microorganism detection and analysis system according to the present invention.
FIG. 2 is a schematic view showing the structure of the microorganism detection and analysis system of the present invention.
FIG. 3 is a schematic view of a transfer module of the present invention.
FIG. 4 is a schematic view showing the internal principle of the culture chamber of the present invention.
Fig. 5 is a schematic view of the tray of the present invention.
In the figure: 1-an inoculation chamber; 11-isolation gloves; 2-a transition bin; 21-a first linear slide; 22-a turntable; 23-a clamping unit; 3-a culture chamber; 31-a pallet rack; 32-a tray; 33-placing the mouth; 34-an image pickup unit; 35-a second linear slide; 36-a lifting platform; 37-a third linear slide; 38-adsorption unit.
Detailed Description
Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the drawings are illustrative and intended to be illustrative of the invention and are not to be construed as limiting the invention. The components of embodiments of the present invention generally described and illustrated in the figures herein may be arranged and designed in a wide variety of different configurations.
In the description of the embodiments of the present invention, it should be noted that the indication of the orientation or the positional relationship is based on the orientation or the positional relationship shown in the drawings, or the orientation or the positional relationship which is usually placed when the product of the present invention is used, or the orientation or the positional relationship which is conventionally understood by those skilled in the art, is only for the convenience of describing the present invention and simplifying the description, and does not indicate or imply that the device or the element which is indicated must have a specific orientation, be constructed in a specific orientation and operate, and thus, cannot be construed as limiting the present invention. Furthermore, the terms "first" and "second" are used merely to distinguish one description from another, and are not to be construed as indicating or implying relative importance.
In the present invention, unless otherwise expressly specified or limited, the terms "mounted," "connected," "secured," and the like are to be construed broadly and can, for example, be fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements.
As shown in fig. 1 to 5, the microorganism detection and analysis system of the present embodiment includes an inoculation chamber 1, a transition bin 2 and a culture chamber 3, wherein the inoculation chamber 1 is provided with an isolation glove 11, the inoculation chamber 1 is a closed space, and is separated from an external environment to maintain an aseptic environment, and the inoculation chamber 1 is used for completing inoculation of a strain to be detected in a specific space and giving identity information of the strain to be detected; an observation window and a pair of isolation gloves 11 are arranged on the chamber body of the inoculation chamber 1, and the workers perform operations such as inoculation and the like in the inoculation chamber 1 through the isolation gloves 11. The two ends of the transition bin 2 are respectively connected with the inoculation chamber 1 and the culture chamber 3, the transition bin 2 is kept closed, and the transition bin 2 is used for safely conveying a culture dish inoculated with the microorganism of the strain to be detected to the culture chamber 3; be equipped with in the transition storehouse 2 and transport the subassembly, transport the subassembly and be used for transporting the good culture dish of inoculation room 1 to the entrance of cultivateing room 3, as shown in fig. 3, transport the subassembly and include first linear sliding table 21, be equipped with revolving stage 22 on the slider of first linear sliding table 21, be equipped with the centre gripping unit 23 that is used for snatching and unclamps the culture dish on revolving stage 22.
The clamping unit is two clamping arms which can be close to and far away from each other and controlled by an air cylinder, or two clamping arms which can be close to and far away from each other and controlled by a bidirectional screw rod matched with two nuts with opposite rotating directions.
The culture chamber is a closed space, is separated from the external environment, realizes the control of the environment in the culture chamber through a temperature and humidity adjusting component and the like, and is beneficial to the growth of microorganisms. Cultivate indoor 3 and be equipped with lifting unit, lifting unit includes two straight line slip tables 35 of vertical and parallel arrangement, and the slider of two straight line slip tables 35 of second all links to each other with elevating platform 36 for on the manipulator that snatchs and unclamp tray 32 located elevating platform 36, the slider of two straight line slip tables 35 of second passes through the synchronizing bar and links to each other, so that better keeping synchronization.
The relative both sides of lifting unit are equipped with tray frame 31, and tray 32 is from last down placing on tray frame 31, and the last a plurality of supporting bodies that down are equipped with of tray frame, and the tray slides and places on the supporting body, and can break away from the supporting body under the pulling force effect. Each tray 32 is provided with a plurality of placing openings 33, and each placing opening 33 can be used for placing a culture dish. The camera unit 34 is disposed at the bottom of the tray rack 31, and the camera unit 34 can move back and forth along the direction of the tray 32 so as to take a picture of the culture dish on each placing port 33.
A plurality of limiting columns are arranged on the periphery of the placing opening 33, and the upper ends of the limiting columns are hemispherical surfaces. When the culture dish was placed on placing the mouth, spacing post can carry on spacingly to the culture dish, prevents that it from removing, and the setting of hemispherical surface is more convenient for the culture dish and is put into along spacing post and place on the mouth.
In this embodiment, the manipulator includes a third linear sliding table 37, two adsorption units 38 for adsorbing and releasing the tray are oppositely arranged on a slider of the third linear sliding table 37, and the adsorption units 38 are driven by the third linear sliding table 37 to be close to the tray racks 31 on both sides, so that the tray 32 in the corresponding position is pulled out by the drive of the third linear sliding table 37 after being adsorbed, and reaches the set position after being driven by the lifting assembly, and similarly, the third linear sliding table 37 can also drive the adsorption unit 38 to push the tray into the tray rack. The tray is made of iron, and the front end of the adsorption unit is provided with an electromagnet.
In other embodiments, the robot includes a turntable provided with an adsorption unit for adsorbing and releasing the trays. The rotary table can drive the adsorption unit to rotate 180 degrees to realize the grabbing of the trays on the two sides.
Cultivate room 3 and also be equipped with the transportation subassembly in, transport the subassembly and be located the lifting unit top, when the manipulator grabs the tray and gets the highest position, transport the subassembly and transport the tray with the culture dish from the entry of cultivating room 3 to and transport the export of cultivating room 3 with the culture dish on the tray.
The working process of the microorganism detection and analysis system is as follows:
the staff places the culture dish on the transportation subassembly of transition storehouse 2 through isolation gloves 11 after inoculation room 1 carries out the inoculation operation to the culture dish, transports the subassembly and transports the culture dish to the entrance of cultivateing room 3, and the subassembly is transported the culture dish from the entrance of cultivateing room 3 to the lifting unit top to the transportation subassembly in the cultivateing room 3 this moment. The manipulator adsorbs a tray and removes to the top along with lifting unit this moment, transports the subassembly and places the culture dish on the mouth of placing of the correspondence of this tray, and after the culture dish on the tray was placed fully, the elevating platform was down, moves to setting for the height after, and the manipulator pushes the tray frame with the tray and loosens.
The culture dishes placed in the same tray are sequentially placed, the interval time is short, photographing records can be carried out simultaneously, when the placing time of the culture dishes in the cabinet body reaches the set time, the lifting platform is lifted and aligned with the tray where the culture dishes to be photographed are placed, the mechanical arm stretches out of the grabbing tray and is pulled back, then the lifting platform is lowered to the bottom, the mechanical arm stretches out to place the tray into the bottom of the tray rack, at the moment, the tray is located above the photographing unit 34, the photographing unit 34 can move along the direction of the tray, and photographing records are carried out on each culture dish sequentially; after the photographing, the mechanical arm again grabs the tray and pulls back, then the lifting platform rises to the top, and the transportation assembly sequentially grabs the culture dish and transports to the export of cultivateing the room.
The microorganism detection and analysis system further comprises an artificial intelligence module, a code scanning unit, an input unit, a processing module, a report generating module and a printer, wherein the processing module is electrically connected with the camera shooting unit, the artificial intelligence module, the code scanning unit, the input unit and the printer respectively.
The input unit and the printer are arranged in the inoculation chamber 1, the input unit is a keyboard and is used for inputting information and instructions to the processing module, the printer is used for printing a two-dimensional code, and the two-dimensional code comprises a unique code of each culture dish and inoculation information; inoculation information includes, but is not limited to, inoculation time, inoculum information, inoculation material information, and incubation time. The printed two-dimensional code is pasted on a culture dish in the inoculation room 1. Sweep the entrance that the sign indicating number unit located cultivation room 3, transfer the culture dish that the subassembly will vaccinate indoor 1 interior inoculation and pasted the two-dimensional code and transfer to cultivation room 3 after the entry, sweep the sign indicating number unit and discern the two-dimensional code, and the two-dimensional code information transmission who will discern to processing module, processing module distributes to the position of placing that corresponds the culture dish correspondence, and record its time of arriving the cultivation room, the time of beginning for the cultivation this moment, it is concrete, processing module has recorded each line and each row's the positional information who places the mouth, positional information is including placing the concrete position of mouth, and place the mouth and whether have and place the culture dish, if place the culture dish, then be related with the two-dimensional code and the positional information of culture dish.
The artificial intelligence module is used for carrying out identification processing on the image collected by the camera unit 34 to obtain the colony quantity and the colony morphology, and the colony type is obtained according to the colony morphology. And the report generation module is used for calling a report template, and filling the information obtained by processing of the artificial intelligence module and the information obtained by recognition of the code scanning unit into the report template to obtain an analysis report. The contents of the analysis report include, but are not limited to, colony number, colony type, incubation time, and name of the inoculator.
The invention is not limited to the above alternative embodiments, and any other various forms of products can be obtained by anyone in the light of the present invention, but any changes in shape or structure thereof, which fall within the scope of the present invention as defined in the claims, fall within the scope of the present invention.
Claims (10)
1. A microbiological detection analysis system comprising:
the device comprises a culture chamber, a camera shooting unit and a storage box, wherein the culture chamber is internally provided with a tray for placing a culture dish and the camera shooting unit for shooting the culture dish;
the artificial intelligence module is used for processing the images acquired by the camera unit to obtain the number and the morphology of the bacterial colonies and obtain the bacterial colony types according to the morphology of the bacterial colonies;
the code scanning unit is arranged at an inlet of the culture chamber and used for identifying the two-dimensional code on the culture dish;
the processing module is respectively electrically connected with the camera shooting unit, the artificial intelligence module and the code scanning unit, and the processing module allocates the two-dimensional codes identified by the code scanning unit to the corresponding placing positions of the corresponding culture dishes.
2. The microorganism detection and analysis system according to claim 1, wherein: the report generation module is used for calling the report template and filling the information obtained by processing of the artificial intelligence module and the information obtained by recognition of the code scanning unit into the report template to obtain an analysis report.
3. The microorganism detection and analysis system according to claim 1, wherein: the culture room is internally provided with a lifting assembly, the lifting assembly is provided with a manipulator for grabbing trays, two opposite sides of the lifting assembly are provided with tray racks, the trays are placed on the tray racks from top to bottom, and each tray is provided with a plurality of placing openings; the bottom of tray frame is located to the unit of making a video recording.
4. The microorganism detection and analysis system according to claim 3, wherein: the lifting assembly comprises two vertical linear sliding tables arranged in parallel, sliding blocks of the two linear sliding tables are connected with the lifting table, and the manipulator is arranged on the lifting table.
5. The microorganism detection and analysis system according to claim 4, wherein: the manipulator comprises a linear sliding table, and two adsorption units for adsorbing and loosening the tray are arranged on a sliding block of the linear sliding table in a back-to-back manner.
6. The microorganism detection and analysis system according to claim 4, wherein: the manipulator comprises a rotary table, and an adsorption unit for adsorbing and loosening the tray is arranged on the rotary table.
7. The microorganism detection and analysis system according to claim 3, wherein: the transfer assembly is arranged in the culture chamber and is positioned above the lifting assembly.
8. The microorganism detection and analysis system according to claim 1, further comprising:
the inoculation room is provided with isolating gloves, a printer is arranged in the inoculation room and used for printing a two-dimensional code, and the two-dimensional code comprises a unique code of each culture dish and inoculation information;
transition storehouse, the both ends in transition storehouse link to each other with inoculation room and cultivation room respectively, are equipped with in the transition storehouse and transport the subassembly.
9. The microorganism detection analysis system according to claim 7 or 8, characterized in that: the transfer assembly comprises a linear sliding table, a rotary table is arranged on a sliding block of the linear sliding table, and a clamping unit used for grabbing and loosening culture dishes is arranged on the rotary table.
10. The microorganism detection and analysis system according to claim 8, wherein: the inoculation information comprises inoculation time, inoculation personnel information and inoculation material information.
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CN202211187104.9A CN115386455A (en) | 2022-09-28 | 2022-09-28 | Microorganism detection and analysis system |
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CN202211187104.9A CN115386455A (en) | 2022-09-28 | 2022-09-28 | Microorganism detection and analysis system |
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