CN219792975U - Automatic bacterial colony culture counting system - Google Patents

Abstract

The utility model discloses an automatic colony culture counting system, which comprises: the operation platform is provided with a plurality of work rest placing stations and is provided with a photographing station; the whole observation recording process is automated by controlling the operations of the code scanning station, the culture dish identification module and the photographing station; through the arrangement of work stations is placed to the work or material rest, realize in limited space, place more culture dish at the same time.

Description

Automatic bacterial colony culture counting system

Technical Field

The utility model relates to the technical field of colony counting, in particular to an automatic colony culture counting system.

Background

The microorganism detection is a legal inspection project of various products such as medicines, foods, cosmetics and the like, and is divided into sterility inspection and microorganism limit inspection. The traditional method for checking the limit of microorganisms is to inoculate a sample into a solid culture medium for culture, and the microorganisms grow and proliferate under the conditions to form colonies with specific morphological characteristics, and counting is completed manually after the culture is finished, so that whether the total number of the colonies meets the regulation standard is judged. The existing colony counter realizes mechanical control, but most of the colony counters are complex in structure, large in size and small in number of storable culture dishes.

For example, chinese patent CN210215368U, publication date 2020, month 03 and 31, comprises a feeding frame, a counting unit, a receiving frame, a trolley and a driving control unit. The scheme has the defects of complex structure, large volume, small quantity of storable culture dishes, incapability of constant-temperature culture, incapability of automatic cycle counting, no culture dish coding recognition function and incapability of storing all process data of colony growth.

Disclosure of Invention

The utility model aims to solve the technical problems that: the current colony counter can store a small number of culture dishes in a limited space. An automatic colony culture counting system is provided, which effectively improves the number of culture dishes which can be stored in a colony counter at the same time.

In order to solve the technical problems, the utility model adopts the following technical scheme: an automatic colony culture counting system comprising: the operation platform is provided with a plurality of work rest placing stations, and is provided with a photographing station.

An automatic bacterial colony culture counting system, operating platform wholly presents rectangular form, 24 work or material rest place the station on it, wherein 20 work or material rest place the station arrange for every row five, four rows altogether, be rectangular form evenly arranged, left 4 edges that are in operating platform, present square shape evenly arranged, every work or material rest place the station all have its peculiar serial number, the biggest circular culture dish of depositing 20 diameters 90mm on every work or material rest place the station, this kind of arrangement mode can place more culture dishes in limited space, improve space utilization, 4 work or material rest place the station next door of constituteing the square be equipped with sweep a yard station, shoot station and culture dish identification module, sweep a yard station and shoot station and be located same row, sweep a yard station and culture dish identification module and be located same row.

Preferably, an XZ two-axis manipulator is arranged on one side of the operation platform, which is close to the photographing station, and a culture dish transferring electric claw capable of clamping a single culture dish is arranged on a Z-axis sliding table of the XZ two-axis manipulator; and an XYZ three-axis manipulator is arranged above the operation platform, and a material rack carrying electric claw is arranged on the XYZ three-axis manipulator. The top of the automatic colony culture counting system is an XYZ three-axis manipulator with a moving range capable of covering the whole range of the operating platform, and a material rack carrying electric claw capable of clamping and carrying a material rack for loading a culture dish is arranged on a Z-axis sliding table of the XYZ three-axis manipulator.

Preferably, the moving range of the rack carrying electric claw covers the whole operating platform, and the rack carrying electric claw is provided with a plurality of fingers for clamping the culture dish loading rack. The work or material rest transport electric claw realizes the displacement on the whole operation platform through the movement of the XYZ three-axis manipulator on the XYZ three axes, and can clamp and get the culture dish loading work or material rest on any work or material rest placing station on the operation platform.

Preferably, the work station is placed to the work station and is equipped with a plurality of cylindric lock, pass through on the work station is placed to the work station cylindric lock with the culture dish loading work or material rest is connected. The culture dish loading rack is provided with a plurality of culture dish limiting columns perpendicular to the operating platform. Three cylindrical pins showing regular triangles are arranged on the work position of the material rest, three corresponding positioning holes are formed in the bottom of the culture dish loading material rest, the culture dish loading material rest is arranged on the work position of the material rest through the combination, three culture dish limiting posts are further vertically arranged on the culture dish loading material rest, and the culture dish can be limited by the three culture dish limiting posts.

Preferably, the bottom of the operation platform is connected with a camera lens module, and the camera lens module is connected with the photographing station. The top of the photographing station is an optical glass platform, the culture dish is arranged on the upper surface of the glass during photographing, a round photographing backboard attached with a black curtain is arranged above the glass, an annular light source is abutted to the lower surface of the glass, the culture dish is illuminated upwards, a camera lens module which is fixedly installed is arranged below the annular light source, and the lens photographs the culture dish arranged on the glass upwards from the bottom.

Preferably, the culture dish identification module is fixed on the operation platform through a support frame, and the culture dish identification module can adjust the angle up and down. The code scanning station is a rotary platform driven by a direct current motor, the direct current motor is used as a driving source, the direct current motor is not shown, a culture dish identification module which is fixedly installed is arranged on the outer side of the code scanning station and used for identifying coding information of a culture dish, and the culture dish identification module can adjust angles up and down.

Preferably, the material rack placing stations have at least one hollow middle rotating row, and each material rack placing station has a different number. The 24 culture dish loading material racks on the operation platform are numbered 1-24, and before initial starting, the 1-number material rack placing station is empty, namely the station can not place the culture dish loading material racks, the 24-number material rack placing station needs to place a culture dish loading material rack without a culture dish as a transit column, and other 2-23-number material rack placing stations can freely select whether the culture dish loading material racks and the number of the culture dishes placed by the material racks are placed at the station.

Preferably, the photographing station comprises an optical glass platform, a photographing backboard is arranged above the optical glass platform, and a black curtain is attached to the photographing backboard. The top of the photographing station is an optical glass platform, the culture dish is arranged on the upper surface of the glass during photographing, a round photographing backboard attached with a black curtain is arranged above the glass, an annular light source is abutted to the lower surface of the glass, the culture dish is illuminated upwards, a camera lens module which is fixedly installed is arranged below the annular light source, and the lens photographs the culture dish arranged on the glass upwards from the bottom.

The utility model has the following substantial effects: according to the utility model, the movement of the XYZ three-axis mechanical arm and the XZ two-axis mechanical arm is controlled by a program, the automatic movement of the culture dish is realized, the operation of the code scanning station, the photographing station and the culture dish identification module is controlled, the automation of the whole observation recording process is realized, the operation is simple, the labor is saved, and more culture dishes are placed in a limited space at the same time through the arrangement of the work rest placement stations.

Drawings

FIG. 1 is a schematic diagram of an embodiment;

FIG. 2 is a front view of an embodiment;

FIG. 3 is a bottom view of an embodiment;

FIG. 4 is a rack placement station numbering plan of an embodiment;

FIG. 5 is a front view of a culture dish loading rack of an embodiment;

FIG. 6 is a bottom view of the dish loading rack of the embodiment.

Wherein: 1. the device comprises an operation platform, 2 XYZ three-axis manipulators, 3 XZ two-axis manipulators, 4, a work station for placing a work rack, 5, a culture dish loading work rack, 6, a code scanning work station, 7, a culture dish identification module, 8, a photographing work station, 9, a culture dish transferring electric claw, 10, a work rack carrying electric claw, 11, a cylindrical pin, 12, a culture dish limiting column, 13, a sliding rail, 14 and a camera lens module.

Detailed Description

The following description of the embodiments of the present utility model will be made with reference to the accompanying drawings.

An automatic colony culture counting system is shown in figures 1, 2, 3 and 4, an XYZ three-axis manipulator 2 with a moving range capable of covering the whole range of an operation platform 1 is arranged at the top of the automatic colony culture counting system, a rack carrying electric claw 10 capable of clamping and carrying culture dish loading racks 5 is arranged on a Z-axis sliding table of the XYZ three-axis manipulator, and each rack can be stacked to store up to 20 circular culture dishes with the diameter of 90mm, and can be integrally placed after stacking the culture dishes outside equipment. The operation platform is provided with 24 work rest placing stations 4, a yard scanning station 6 and a photographing station 8. The installation centers of the code scanning station, the photographing station, the No. 1 material rack placing station and the No. 24 material rack placing station are on the same straight line, an XZ two-axis manipulator 3 is installed at the rear part of the code scanning station, the X axis of the two-axis manipulator is parallel to the connecting straight line, and a culture dish transferring electric claw 9 capable of clamping a single culture dish is installed on a sliding table of the Z axis. One end of the X axis of the XYZ three-axis manipulator is connected with a sliding rail 13 for supporting.

The code scanning station is a rotating platform driven by a direct current motor, the direct current motor is used as a driving source, the direct current motor is not shown, a culture dish identification module 7 fixedly installed through a support frame is arranged on the inner side of the code scanning station and used for identifying coded information of the culture dishes, and the culture dish identification module can adjust the angle according to actual conditions so as to determine that the culture dish identification module can scan the coded information of the culture dishes located on the code scanning station.

The uppermost of the photographing station is an optical glass platform, the culture dish is arranged on the upper surface of the glass during photographing, a round photographing backboard attached with a black curtain is arranged above the glass, and the annular light source abuts against the lower surface of the glass to upwardly illuminate the culture dish. A fixedly mounted camera lens module 14 is arranged below the annular light source, and the lens shoots upwards from the bottom a petri dish placed on the glass.

As shown in fig. 4, the 24 rack placement stations on the operating platform are numbered 1-24. Before initial starting, the 1 st work station should be empty, namely this work station can not place the culture dish loading work or material rest, and 24 st work station needs to place a culture dish loading work or material rest that does not contain the culture dish as transit, and the rest 2-23 st work or material rest place work or material rest and can freely select whether this work station places culture dish loading work or material rest and the culture dish quantity of placing on the work or material rest.

As shown in fig. 5 and 6, three cylindrical pins 11 presenting a regular triangle are arranged on the work position of the material rack, three corresponding positioning holes are arranged at the bottom of the material rack for loading the culture dish, the culture dish loading material rest is installed on the material rest placing station through the combination, and three culture dish limiting columns 12 are further vertically arranged on the culture dish loading material rest and can limit the culture dishes.

During operation, the XYZ triaxial manipulator of top installation is taken the work or material rest and is carried the electric claw and put the work or material rest clamp that the work or material rest was placed to the work or material rest and is got and transfer to the work or material rest of No. 1, and work or material rest place the work or material rest of No. 2 this moment and be empty. The Z axis of XZ two-axis manipulator drives the culture dish that installs above that shifts electric claw and is in safe height in advance, the X axis drives the Z axis and removes to 1 work or material rest place the station directly over, the Z axis drives electric claw and drops to 1 work or material rest place work or material rest the uppermost culture dish position, electric claw presss from both sides the tight culture dish of grabbing, the Z axis drives the electric claw that clamps and takes the culture dish of waiting at present and lifts to safe high position, XZ two-axis manipulator drives electric claw and removes and drop to the position of sweeping a certain height in the center of a yard station tray, and Zhang Kaidian claw places the culture dish on sweeping a yard station tray, XZ two-axis manipulator keeps motionless this moment, the electric claw is opened always, then the tray is carried the culture dish and is rotated slowly around its center axis, until the culture dish identification module can scan the two-dimensional code information that is located the culture dish side, this flow is used for reading the parameter information of current culture dish. After the code scanning is completed, the electric claw clamps again to grasp the culture dish, the XZ two-axis mechanical arm drives the electric claw which clamps the culture dish after the code scanning is completed to be lifted to a safe height, the XZ two-axis mechanical arm moves to a position with a certain height at the center of the photographing station, after the electric claw is opened in place, the culture dish is placed on the photographing tray optical glass platform, at the moment, the annular light source is opened, the camera triggers photographing, and the colony growth photo of the current culture dish is obtained. Then the electric claw clamps and grabs the culture dish again, the XZ two-axis mechanical arm drives the electric claw which clamps the culture dish which is photographed at present to rise to a safe high position again, the electric claw is transferred to the position right above the 24-size work rest placing station, the Z axis descends after the electric claw is in place, the clamping claw is loosened, and the culture dish is placed on the 24-size work rest placing station. And sequentially circulating until all the culture dishes on the culture dish loading material racks placed by the material rack placing station 1 are transferred to the culture dish loading material racks placed by the material rack placing station 24, and at the moment, the culture dish loading material racks placed by the material rack placing station 1 do not place the culture dishes, and the culture dish loading material racks placed by the material rack placing station 24 are fully loaded.

The code scanning station, the photographing station, the No. 1 work rest placing station, the No. 24 work rest placing station and the XZ two-axis mechanical arm are matched, so that transfer of the culture dish between the two stations can be completed, and the purposes of code identification and photo acquisition of culture dish samples placed by a single work rest are achieved.

Meanwhile, the XYZ three-axis manipulator is used for taking the material rack placed by the material rack placing station to clamp and transfer the material rack placed by the material rack placing station to the material rack placing station 2, the material rack placing station 3 is empty at the moment, the XYZ three-axis manipulator is used for taking the material rack placed by the material rack placing station to clamp and transfer the material rack placed by the material rack placing station 4 to the material rack placing station 3, the material rack placing station 4 is empty at the moment, the material rack placing stations 5-23 are sequentially completed to transfer, and the material rack placing station 23 is empty at the last. And finally transferring the culture dish loading material racks placed by the 24-number material rack placing station for completing the transfer of the culture dishes to the 23-number material rack placing station, transferring the loading material racks without the culture dishes placed by the 1-number material rack placing station to the 24-number material rack placing station, completing the transfer after photographing of a single material rack, and carrying out photographing transfer of the next material rack. And sequentially circulating to achieve the purpose of circularly and continuously acquiring all the growth pictures of the culture dishes of the material rack at fixed time intervals.

The timing of the batch culture mode operation is briefly described as follows: firstly, manually and sequentially using a code scanning gun to scan codes to input information of the culture dishes which are inoculated and labeled with the two-dimensional code into a system, and then stacking and loading the input culture dishes on a culture dish loading rack, wherein the loading directions are required to be consistent. After loading is completed, the material rack is placed on a material rack placing station of the system culture dish material rack loading No. 2-23. And after the culture parameters are set on the operation screen, the culture can be started. Workflow during incubation with reference to the above description, photographs taken at the photographing station will be automatically analyzed by the computer background to locate growing colonies based on changes in the front and rear pictures, and culture dishes may also be added during incubation. The latest photo and the current colony number of the culture dish in the current system are displayed on an operation interface in real time, and the algorithm can automatically divide the adhered colonies and automatically realize picture registration. Continuously counting in the whole culture period, outputting a colony number growth curve, automatically generating a result report, and storing a colony growth image. The automatic batch microorganism culture counting can be realized, the whole culture data process can be traced back, the data management and analysis can be easily carried out by experimenters, and the remote supervision can be realized.

The foregoing examples illustrate only a few embodiments of the utility model, which are described in detail and are not to be construed as limiting the scope of the utility model. It should be noted that it will be apparent to those skilled in the art that several variations and modifications can be made without departing from the spirit of the utility model, which are all within the scope of the utility model.

Claims (8)

1. An automated colony culture counting system, comprising: the operation platform is provided with a plurality of work rest placing stations and is provided with a photographing station; the whole operating platform presents as the rectangle form, has distributed a plurality of work or material rest and has placed the station on it, and wherein partial work or material rest is placed the station and is arranged for every row a plurality of, is the rectangle form and evenly arranges, and the work or material rest that remains in operating platform's edge is placed the station and is presented square form and evenly arranges, sweeps a yard station and take a picture the station and be located same row, sweeps a yard station and culture dish identification module and be located same row.

2. The automatic colony culture counting system according to claim 1, wherein an XZ two-axis manipulator is arranged on one side, close to the photographing station, of the operation platform, a culture dish transferring electric claw is arranged on the XZ two-axis manipulator, an XYZ three-axis manipulator is arranged above the operation platform, and a rack carrying electric claw is arranged on the XYZ three-axis manipulator.

3. The automated colony culture counting system of claim 2, wherein the range of movement of the rack handling electrical gripper covers the entire operating platform, the rack handling electrical gripper being provided with a plurality of fingers for gripping a culture dish loading rack.

4. The automatic colony culture counting system of claim 1, wherein the work rest placing work station is provided with a plurality of cylindrical pins, the work rest placing work station is connected with a culture dish loading work rest through the cylindrical pins, and the culture dish loading work rest is provided with a plurality of culture dish limiting columns perpendicular to the operation platform.

5. The automatic colony culture counting system according to claim 1 or 2, wherein a camera lens module is connected to the bottom of the operation platform, and the camera lens module is connected to the photographing station.

6. The automatic colony culture counting system of claim 1, wherein the culture dish identification module is fixed on the operating platform by a support frame, and the culture dish identification module can adjust the angle up and down.

7. An automated colony culture counting system according to claim 1 or claim 2, wherein the rack placement stations have at least one empty middle row, each of the rack placement stations having a different number.

8. The automatic colony culture counting system according to claim 1 or 2, wherein the photographing station comprises an optical glass platform, a photographing backboard is arranged above the optical glass platform, and a black curtain is attached to the photographing backboard.