CN115418308A - Microbial cultivation analytical equipment - Google Patents
Microbial cultivation analytical equipment Download PDFInfo
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- CN115418308A CN115418308A CN202211247288.3A CN202211247288A CN115418308A CN 115418308 A CN115418308 A CN 115418308A CN 202211247288 A CN202211247288 A CN 202211247288A CN 115418308 A CN115418308 A CN 115418308A
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- 230000000813 microbial effect Effects 0.000 title description 5
- 230000007246 mechanism Effects 0.000 claims description 13
- 238000009629 microbiological culture Methods 0.000 claims description 9
- 238000010438 heat treatment Methods 0.000 claims description 3
- 239000000463 material Substances 0.000 claims description 3
- 244000005700 microbiome Species 0.000 abstract description 23
- 238000000034 method Methods 0.000 description 7
- 230000008569 process Effects 0.000 description 7
- 241000894006 Bacteria Species 0.000 description 3
- 238000011109 contamination Methods 0.000 description 3
- 230000005540 biological transmission Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 230000009471 action Effects 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 210000004349 growth plate Anatomy 0.000 description 1
- 238000003384 imaging method Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/52—Mobile; Means for transporting the apparatus
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/46—Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/48—Automatic or computerized control
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Abstract
The invention relates to a microorganism culture device, and provides a microorganism culture analysis device for better photographing and recording the growth condition of microorganisms, which comprises a box body, a plurality of culture plates distributed in the box body along the vertical direction, a plurality of slide rails arranged on the inner wall of the box body, a fixed block arranged at the upper end in the box body, a photographing device arranged on the lower side of the fixed block, a pair of grabbing devices arranged at two opposite sides of the fixed block, a first driving device used for driving the fixed block to move along the vertical direction, and a second driving device used for driving the fixed block to move along the horizontal direction; the both ends of the culture plate are arranged in the slide rails in a sliding mode, and the culture plate comprises a first culture plate and a second culture plate which are arranged on two opposite sides of the fixed block respectively. The microorganism culture analysis device can efficiently and automatically take pictures of the culture dish in the box body and can obviously reduce the manual use.
Description
Technical Field
The invention relates to the technical field of microorganism culture devices, in particular to a microorganism culture analysis device.
Background
In various research processes of microorganisms, the microorganisms are cultured in a limited environment, so that the growth and reproduction conditions of the microorganisms are observed as an important link in the research process. The common microbial culture is mainly to uniformly coat microbial strains on the surface of a solid medium (or disperse the microbial strains in liquid culture), and after a certain time, the microorganisms can produce and propagate into individual colonies on the surface of the solid medium, and at the moment, the colonies need to be photographed and then counted, observed and the like.
The traditional colony is photographed one by experimenters using a camera or the like to shoot the colony towards a culture dish, and then counted and analyzed. For some laboratories with a certain degree of automation, it is possible to set up a camera in the incubator, in particular: the culture plate that will load the culture dish is pulled out, then pulls down the shooting device of incubator top, aims at the culture dish, in turn shoot can, so not only efficiency is higher, need not take out the culture dish completely moreover, can realize the continuous culture of microorganism to a certain extent. However, the manual operation is still needed, and the incubator needs to be opened, which has certain influence on the growth of the microorganisms.
In conclusion, the design of a microbial cultivation and photographing device capable of realizing high automation can ensure the stability of the growth state of the microbes to a certain extent and can also obviously reduce manual operation.
Disclosure of Invention
The invention aims to provide a microorganism culture analysis device which can efficiently and automatically take pictures of culture dishes in a box body and can remarkably reduce the use of manpower.
The embodiment of the invention is realized by the following technical scheme: the invention discloses a microorganism culture and analysis device, which comprises a box body, a plurality of culture plates distributed in the box body along the vertical direction, a plurality of slide rails arranged on the inner wall of the box body, a fixed block arranged at the upper end in the box body, a shooting device arranged at the lower side of the fixed block, a pair of grabbing devices arranged at two opposite sides of the fixed block, a first driving device used for driving the fixed block to move along the vertical direction, and a second driving device used for driving the fixed block to move along the horizontal direction; the both ends of the culture plate are slidably arranged in the sliding rails, and the culture plate comprises a first culture plate and a second culture plate which are respectively arranged at the two opposite sides of the fixed block.
Furthermore, the gripping device comprises a telescopic mechanism fixedly connected with the fixed block and an electromagnet arranged at the end part of the movable end of the telescopic mechanism; a first magnetic attraction plate is arranged on one side, away from the second culture plate, of the upper side of the first culture plate, and a second magnetic attraction plate is arranged on one side, away from the first culture plate, of the upper side of the second culture plate; the first magnetic attraction plate and the second magnetic attraction plate are made of materials capable of being attracted by magnets.
Furthermore, the telescopic mechanism comprises a first electric telescopic cylinder, and a telescopic rod of the first electric telescopic cylinder is fixedly connected with the fixing block.
Further, the telescopic rod of the first electric telescopic cylinder is a multi-section telescopic rod.
Further, the first driving device comprises a second electric telescopic cylinder vertically arranged at the upper end inside the box body; and the telescopic rod of the second electric telescopic cylinder is fixedly connected with the fixed block.
Furthermore, the second driving device comprises a sliding block fixedly arranged on the side wall of the second electric telescopic cylinder, a threaded hole formed in the sliding block, a sliding hole formed in the sliding block, a screw rod in threaded connection with the threaded hole, a motor for driving the screw rod to rotate, and a sliding rod in sliding connection with the sliding block; the two ends of the sliding rod are connected with the side wall of the box body, and the two ends of the screw rod are rotatably connected with the side wall of the box body; the axial direction of the slide rod is parallel to the axial direction of the screw rod, and the axial direction of the slide rod is parallel to the length direction of the culture plate. In addition, the transmission mode of the electric telescopic cylinder, the screw rod and the like can be changed into a transmission mode of driving by using a chain, the chain is driven by a motor and a gear, and the fixed block is driven by the chain to move up and down and move left and right, wherein the independent chain driving mechanism can be used for moving up and down and moving left and right.
Furthermore, the first magnetic attraction plate is arranged in the middle of the first culture plate along the length direction, and the second magnetic attraction plate is arranged in the middle of the second culture plate along the length direction.
Furthermore, a heating device and a humidifying device are further arranged inside the box body.
The technical scheme of the embodiment of the invention at least has the following advantages and beneficial effects: according to the microorganism culture analysis device, when the culture dish is placed on the culture plate in use, when the bacteria growth condition in the culture plate needs to be photographed and recorded after a certain time, the first driving device can be used for driving the fixing block to move downwards for a certain distance, then the grabbing device is used for grabbing the first culture plate (or the second culture plate) on one layer between the first culture plate and the second culture plate, namely under the fixing block, so that the shooting device (camera) on the lower side of the fixing block can be just aligned to the culture dish, then shooting can be carried out, all culture dishes on the culture plate can be shot under the action of the second driving device, then the culture plate is returned to the previous position, and the step can be repeated, so that the invalid volume in the box body is smaller, the space utilization rate is better, the whole process can be automatically completed, the box body does not need to be opened, manpower is not needed, and the risk of bacterial contamination is avoided.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings required in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present invention and therefore should not be considered as limiting the scope, and those skilled in the art can also obtain other related drawings based on the drawings without inventive efforts.
FIG. 1 is a schematic structural view of a microorganism culture analysis apparatus according to an embodiment of the present invention;
FIG. 2 is a schematic view showing a configuration of a state of the inside of the microorganism culture analysis apparatus according to the embodiment of the present invention in use;
FIG. 3 is a schematic structural view of two states of the inside of the microorganism culture analysis apparatus according to the embodiment of the present invention in use;
FIG. 4 is a schematic structural view of three internal states of a microorganism culture analysis apparatus according to an embodiment of the present invention in use;
FIG. 5 is a schematic view of the structure of the fixed block of the microorganism culture analysis apparatus according to the embodiment of the present invention.
Icon: 10-a box body, 21-a first culture plate, 22-a second culture plate, 23-a first magnetic suction plate, 24-a second magnetic suction plate, 25-a slide rail, 31-a fixed block, 32-a shooting device, 33-a first electric telescopic cylinder, 34-an electromagnet, 41-a second electric telescopic cylinder, 42-a slide block, 43-a threaded hole, 44-a slide hole, 45-a screw rod, 46-a slide rod and 47-a motor.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all, embodiments of the present invention. The components of embodiments of the present invention generally described and illustrated in the figures herein may be arranged and designed in a wide variety of different configurations.
Thus, the following detailed description of the embodiments of the present invention, as presented in the figures, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
It should be noted that: like reference numbers and letters refer to like items in the following figures, and thus, once an item is defined in one figure, it need not be further defined and explained in subsequent figures.
In the description of the present invention, it should be noted that if the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. indicate an orientation or positional relationship based on the orientation or positional relationship shown in the drawings or the orientation or positional relationship which is usually placed when the product of this application is used, the description is merely for convenience and simplicity of description, and it is not intended to indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention.
In the description of the present invention, it should be further noted that unless otherwise explicitly stated or limited, the terms "disposed," "mounted," "connected," and "connected" should be interpreted broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
Examples
As shown in fig. 1 to 5, the microorganism culture analysis apparatus of this embodiment includes a housing 10, a plurality of culture plates vertically disposed inside the housing 10, a plurality of slide rails 25 disposed on an inner wall of the housing 10, a fixing block 31 disposed at an upper end of the housing 10, a camera 32 disposed at a lower side of the fixing block 31, a pair of grasping devices disposed at opposite sides of the fixing block 31, a first driving device for driving the fixing block 31 to move in a vertical direction, and a second driving device for driving the fixing block 31 to move in a horizontal direction; both ends of the culture plate are arranged in the slide rails 25 in a sliding manner, and the culture plate comprises a first culture plate 21 and a second culture plate 22 which are respectively arranged at two opposite sides of the fixed block 31. Specifically, place the culture dish on the culture plate when using, when need take a picture the record to the bacterium growth condition of culture plate inside after a certain time, can use first drive arrangement drive fixed block 31 to move down a certain distance, then use grabbing device to grab the first culture plate 21 (or second culture plate 22) of one of them layer between first culture plate 21 and second culture plate 22, namely under fixed block 31, make shooting device 32 (camera) of fixed block 31 downside can just aim at the culture dish, then just can shoot, and all culture dishes on the culture plate are all shot under the effect of second drive arrangement and are accomplished, then return this culture plate to the position before, can repeat this step, can accomplish the shooting work of all culture dishes voluntarily, so not only the inside invalid volume of box 10 is littleer, space utilization is better, and whole process can all be accomplished voluntarily, need not open box 10, need not consume the manual work, there is not the risk of contamination. It should be noted that, among other things, the program may be preset so that it automatically completes the shooting process, or the shooting process may be manually controlled.
The gripping device in this embodiment includes a telescopic mechanism fixedly connected to the fixed block 31, and an electromagnet 34 disposed at the end of the movable end of the telescopic mechanism; a first magnetic attraction plate 23 is arranged on one side of the upper side of the first culture plate 21, which is far away from the second culture plate 22, and a second magnetic attraction plate 24 is arranged on one side of the upper side of the second culture plate 22, which is far away from the first culture plate 21; the first magnetic attraction plate 23 and the second magnetic attraction plate 24 are made of a material that can be attracted by a magnet. Specifically, when the first culture plate 21 needs to be grabbed, the telescoping mechanism can be driven to push out the electromagnet 34, so that the electromagnet 34 is close to the first magnetic attraction plate 23, then the electromagnet 34 is electrified to generate magnetic force to attract the first magnetic attraction plate 23 (as shown in fig. 2), the telescoping mechanism pulls back the electromagnet 34, so that the first culture plate 21 is positioned right below the shooting device 32 (as shown in fig. 3), then the electromagnet 34 is powered off, the shooting work can be sequentially performed at the moment (as shown in fig. 4), and after the shooting is completed, the telescoping mechanism pushes the first culture plate 21 back to the end portion of the slide rail 25 through the electromagnet 34 and the first magnetic attraction plate 23 (at the moment, the electromagnet 34 does not need to be electrified). The principle of imaging the second growth plate 22 is the same.
The telescopic mechanism in this embodiment includes a first electric telescopic cylinder 33, and a telescopic rod of the first electric telescopic cylinder 33 is fixedly connected to the fixing block 31. The telescopic rod of the first electric telescopic cylinder 33 is a multi-section telescopic rod. Specifically, only one row of culture dishes can be placed on the culture plate, and the culture plate is not heavy, so that a small or miniature electric telescopic cylinder is used. The multi-section first electric telescopic cylinder 33 can significantly reduce the length of the cylinder body of the first electric telescopic cylinder 33.
The first driving device in this embodiment includes a second electric telescopic cylinder 41 vertically disposed at the upper end inside the box body 10; the telescopic rod of the second electric telescopic cylinder 41 is fixedly connected with the fixed block 31. Specifically, the structure using the second electric telescopic cylinder 41 is simpler, easier to control, and the elevation of the photographing device 32 can be controlled more accurately. Furthermore, screw arrangements or the like may also be used, which requires a corresponding adjustment of the arrangement of the second drive.
The second driving device in this embodiment includes a sliding block 42 fixedly disposed on a side wall of the second electric telescopic cylinder 41, a threaded hole 43 formed in the sliding block 42, a sliding hole 44 formed in the sliding block 42, a screw 45 in threaded connection with the threaded hole 43, a motor 47 for driving the screw 45 to rotate, and a sliding rod 46 in sliding connection with the sliding block 42; two ends of the sliding rod 46 are connected with the side wall of the box body 10, and two ends of the screw rod 45 are rotatably connected with the side wall of the box body 10; the axial direction of the slide bar 46 is parallel to the axial direction of the screw 45, and the axial direction of the slide bar 46 is parallel to the longitudinal direction of the culture plate. Specifically, the horizontal movement of the photographing device 32 is controlled using a structure similar to a reciprocating screw, so that the movement distance of the photographing device 32 can be more precisely controlled, so that the photographing device 32 can be better aligned with the culture dish. In addition, a through hole can be formed in the culture dish, and the culture dish is placed in the through hole, so that the position of the culture dish can be better limited, and shooting can be better performed. During shooting each time, the shooting device 32 only needs to move a set distance, and operations such as identification and alignment of the culture dish by the shooting device 32 are not needed.
The first magnetic attraction plate 23 in this embodiment is provided at the middle of the first culture plate 21 in the longitudinal direction, and the second magnetic attraction plate 24 is provided at the middle of the second culture plate 22 in the longitudinal direction. In particular, this allows better pulling and pushing of the first 21 and second 22 culture plates.
The inside of the box 10 in this embodiment is also provided with a heating device and a humidifying device. Specifically, the conditions necessary for culturing microorganisms at constant temperature and humidity.
To sum up, the microorganism culture analysis device of this embodiment, place the culture dish on the culture plate when using, when need take a picture to the bacterium growth condition in the culture plate inside after a certain period of time and record, can use first drive arrangement drive fixed block 31 to move down a certain distance, then use grabbing device to grab first culture plate 21 (or second culture plate 22) of one of them layer between first culture plate 21 and second culture plate 22, also be exactly under fixed block 31, make shooting device 32 (camera) of fixed block 31 downside just aim at the culture dish, then just can shoot, and all culture dishes on the culture plate all shoot under the effect of second drive arrangement, then return this culture plate to the position before, can, repeat this step, can accomplish the shooting work of all culture dishes voluntarily, so not only the invalid volume in box 10 inside is littleer, space utilization is better, and whole process can all be accomplished automatically, need not open box 10, need not consume the manual work, there is not the risk of contamination.
The above is only a preferred embodiment of the present invention, and is not intended to limit the present invention, and various modifications and changes will occur to those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (8)
1. A microbial culture analysis device, characterized in that: the device comprises a box body, a plurality of culture plates distributed in the box body along the vertical direction, a plurality of slide rails arranged on the inner wall of the box body, a fixed block arranged at the upper end in the box body, a shooting device arranged on the lower side of the fixed block, a pair of grabbing devices arranged on two opposite sides of the fixed block, a first driving device used for driving the fixed block to move along the vertical direction, and a second driving device used for driving the fixed block to move along the horizontal direction;
the two ends of the culture plate are arranged in the sliding rails in a sliding mode, and the culture plate comprises a first culture plate and a second culture plate which are arranged on the two opposite sides of the fixing block respectively.
2. The microbial culture analysis device of claim 1, wherein: the gripping device comprises a telescopic mechanism fixedly connected with the fixed block and an electromagnet arranged at the end part of the movable end of the telescopic mechanism;
a first magnetic suction plate is arranged on one side, away from the second culture plate, of the upper side of the first culture plate, and a second magnetic suction plate is arranged on one side, away from the first culture plate, of the upper side of the second culture plate; the first magnetic attraction plate and the second magnetic attraction plate are made of materials capable of being attracted by magnets.
3. The microbial culture analysis device of claim 2, wherein: the telescopic mechanism comprises a first electric telescopic cylinder, and a telescopic rod of the first electric telescopic cylinder is fixedly connected with the fixed block.
4. The microbial culture analysis device of claim 3, wherein: the telescopic rod of the first electric telescopic cylinder is a multi-section telescopic rod.
5. The microbial culture analysis device of claim 1, wherein: the first driving device comprises a second electric telescopic cylinder vertically arranged at the upper end in the box body; and the telescopic rod of the second electric telescopic cylinder is fixedly connected with the fixed block.
6. The microbial culture analysis device of claim 5, wherein: the second driving device comprises a sliding block fixedly arranged on the side wall of the second electric telescopic cylinder, a threaded hole formed in the sliding block, a sliding hole formed in the sliding block, a screw rod in threaded connection with the threaded hole, a motor used for driving the screw rod to rotate and a sliding rod in sliding connection with the sliding block;
the two ends of the sliding rod are connected with the side wall of the box body, and the two ends of the screw rod are rotatably connected with the side wall of the box body; the axial direction of the slide rod is parallel to the axial direction of the screw rod, and the axial direction of the slide rod is parallel to the length direction of the culture plate.
7. The microbial culture analysis device of claim 2, wherein: the first magnetic attraction plate is arranged in the middle of the first culture plate along the length direction, and the second magnetic attraction plate is arranged in the middle of the second culture plate along the length direction.
8. The microbial culture analysis device of claim 1, wherein: and a heating device and a humidifying device are also arranged in the box body.
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| CN202211247288.3A CN115418308B (en) | 2022-10-12 | 2022-10-12 | Microorganism culture analysis device |
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| CN202211247288.3A CN115418308B (en) | 2022-10-12 | 2022-10-12 | Microorganism culture analysis device |
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| CN115418308B CN115418308B (en) | 2023-07-18 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116144462A (en) * | 2023-04-23 | 2023-05-23 | 四川若斌生物科技有限责任公司 | Push-pull device, full-automatic photographing microorganism incubator and photographing method |
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| CN116144462A (en) * | 2023-04-23 | 2023-05-23 | 四川若斌生物科技有限责任公司 | Push-pull device, full-automatic photographing microorganism incubator and photographing method |
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Denomination of invention: A Microbial Culture Analysis Device Granted publication date: 20230718 Pledgee: Industrial Bank Limited by Share Ltd. Chengdu branch Pledgor: Sichuan Ruobin Biotechnology Co.,Ltd. Registration number: Y2024980003600 |