CN115385457A - Agricultural sewage treatment method - Google Patents

Agricultural sewage treatment method Download PDF

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Publication number
CN115385457A
CN115385457A CN202211079653.4A CN202211079653A CN115385457A CN 115385457 A CN115385457 A CN 115385457A CN 202211079653 A CN202211079653 A CN 202211079653A CN 115385457 A CN115385457 A CN 115385457A
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parts
microbial inoculum
sewage
chlorella
agricultural
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CN202211079653.4A
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Inventor
仇煜臻
仇俊涛
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Yunnan Xingyao Biological Products Co ltd
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Yunnan Xingyao Biological Products Co ltd
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Priority to CN202211079653.4A priority Critical patent/CN115385457A/en
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    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C02F3/341—Consortia of bacteria
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
    • C02F3/322—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
    • C02F3/322—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae
    • C02F3/325—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae as symbiotic combination of algae and bacteria
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C02F3/347—Use of yeasts or fungi
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C02F3/348—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the way or the form in which the microorganisms are added or dosed
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00—Nature of the contaminant
    • C02F2101/30—Organic compounds
    • C02F2101/306—Pesticides
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00—Nature of the contaminant
    • C02F2101/30—Organic compounds
    • C02F2101/40—Organic compounds containing sulfur
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2209/00—Controlling or monitoring parameters in water treatment
    • C02F2209/06—Controlling or monitoring parameters in water treatment pH
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2209/00—Controlling or monitoring parameters in water treatment
    • C02F2209/08—Chemical Oxygen Demand [COD]; Biological Oxygen Demand [BOD]
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2209/00—Controlling or monitoring parameters in water treatment
    • C02F2209/10—Solids, e.g. total solids [TS], total suspended solids [TSS] or volatile solids [VS]
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2209/00—Controlling or monitoring parameters in water treatment
    • C02F2209/14—NH3-N
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2209/00—Controlling or monitoring parameters in water treatment
    • C02F2209/16—Total nitrogen (tkN-N)

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Hydrology & Water Resources (AREA)
  • Environmental & Geological Engineering (AREA)
  • Water Supply & Treatment (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • Ecology (AREA)
  • Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention relates to a method for treating agricultural sewage, which comprises the following steps: inoculating chlorella HL and scenedesmus obliquus in agricultural sewage, culturing for 2-4 days, adding a compound microbial agent containing Bacillus subtilis XF-1, trichoderma viride, bifidobacterium, yeast, lactobacillus, nitrifying bacteria, azotobacter and EM, and continuously culturing for 3-5 days. Aiming at the characteristics of agricultural sewage, the invention reasonably sets the compound microbial agent, and combines with chlorella HL and scenedesmus obliquus inoculation, so that pollutants in the agricultural sewage are pertinently removed, and the pollutants are recycled after quality inspection is qualified, thereby saving resources.

Description

Agricultural sewage treatment method
Technical Field
The invention relates to the technical field of agricultural production, in particular to an agricultural sewage treatment method.
Background
The agricultural sewage is sewage or liquid substance discharged in the processes of crop cultivation, livestock breeding, agricultural product processing and the like and influencing human health and environmental quality. The sources of the sewage mainly comprise farmland runoff, feedlot sewage and agricultural product processing sewage. The sewage contains various pathogens, suspended matters, fertilizers, pesticides, insoluble solids, salts and the like. The agricultural sewage is large in quantity and wide in influence range. Nutrient elements such as nitrogen, phosphorus and the like in the sewage enter water areas such as rivers, lakes, internal sea and the like to cause eutrophication; pesticides, pathogens and other toxic substances can pollute drinking water sources and harm human health; causing large-scale soil pollution and destroying the balance of the ecological system. Compared with industrial sewage, the agricultural sewage has less toxicity and low eutrophication degree compared with domestic sewage, but has the characteristics of toxicity, eutrophication and complex pollutants.
Because the agricultural sewage contains a large amount of nitrogen and phosphorus nutrient substances, the agricultural sewage is utilized to culture microalgae, rich nitrogen and phosphorus can be provided for the growth of the microalgae while water quality is purified, and meanwhile, bacteria in the sewage can decompose organic matters in the sewage to generate carbon dioxide and provide a carbon source required by the growth of the microalgae. Compared with the method of utilizing the artificially prepared culture medium and adding pure carbon dioxide gas to culture the microalgae, the method of utilizing the sewage to culture the microalgae can reduce the culture cost of the microalgae to a certain extent, can rely on the large-scale culture of the existing sewage treatment plant, and is an effective way for solving the water environment pollution and the energy crisis at present. The microalgae and the bacteria have complex interrelations such as intergrowth, antagonism and the like. The symbiotic relationship of bacteria and algae is shown in that oxygen is released by the microalgae through photosynthesis to supply the bacteria to carry out metabolic activity, the bacteria degrade and convert organic pollutants in water into carbon dioxide, inorganic nitrogen, phosphorus compounds and the like to provide carbon sources and nutrients required by the photosynthesis for the microalgae, and the symbiotic relationship among the algae and the bacteria is formed through circulation.
The bacterial-algae antagonistic relationship is shown in that the microalgae can generate antibiotic substances for inhibiting the growth of bacteria, the growth of the microalgae can cause the increase of pH and dissolved oxygen so as to inhibit the growth of certain bacteria, and the bacteria can inhibit or even crack algae cells by releasing extracellular substances such as enzymes capable of dissolving cellulose or the like, or the bacteria are attached to the inner wall of a reactor in the growth process to block the transmission of light, so that the photosynthetic growth of the microalgae is influenced. In order to eliminate the influence of bacteria on microalgae, the sewage is often subjected to sterilization pretreatment in the prior art. However, the sterilization pretreatment also has problems of high cost and difficulty in expansion.
Therefore, how to provide a microbial preparation capable of forming a symbiotic relationship with microalgae is a technical problem to be solved urgently by those skilled in the art.
In patent CN108841767B, an invention previously granted in this application, a compound microbial agent is disclosed, which contains bacillus subtilis XF-1, trichoderma viride, bifidobacterium, saccharomycetes, lactic acid bacteria, nitrobacteria, azotobacter and EM bacteria. The compound microbial agent is mainly applied to microbial fertilizers, but the inventor of the application finds that the compound microbial agent can promote the growth of microalgae in agricultural sewage and can reduce the sewage degree of the sewage in later research, and the invention is completed based on the finding.
Disclosure of Invention
Based on the above background art, the technical problem to be solved by the present invention is to provide a method for treating agricultural wastewater. In order to realize the purpose of the invention, the following technical scheme is adopted:
the invention relates to a method for treating agricultural sewage, which comprises the following steps: inoculating chlorella HL and Scenedesmus obliquus in agricultural sewage, culturing for 2-4 days, adding a compound microbial agent containing Bacillus subtilis XF-1, trichoderma viride, bifidobacterium, yeast, lactobacillus, nitrobacteria, azotobacter and EM, and continuously culturing for 6-10 days.
In a preferred embodiment of the present invention, the ratio of the number of chlorella HL to scenedesmus obliquus is 1:1-5; preferably 1:1.5-2.5.
In a preferred embodiment of the present invention, the total combined amount of Chlorella HL and Scenedesmus obliquus is 0.8-1.5X 10 5 Per mL of wastewater.
In a preferred embodiment of the present invention, the amount of the complex microbial inoculant added is 8-20 g/ton of sewage, preferably 10-15 g/ton of sewage.
In a preferred embodiment of the present invention, in the agricultural wastewater: COD is 800-900mg/L, BOC:800-1000mg/L, TSS (total suspended solids): 600-800mg/L, TN (total nitrogen): 700-800mg/L, ammonia nitrogen 400-800mg/L, pH6.0-6.5, fecal coliform number 0.5-2 x 10 6 L, 3-5mg/L of organophosphorus pesticide and 1-4mg/L of dimethoate.
In a preferred embodiment of the present invention, the complex microbial inoculant comprises: 15-25 parts of bacillus subtilis XF-1 microbial inoculum, 8-12 parts of trichoderma viride microbial inoculum, 6-10 parts of bifidobacterium microbial inoculum, 6-10 parts of yeast microbial inoculum, 4-8 parts of lactobacillus microbial inoculum, 4-8 parts of nitrobacteria microbial inoculum, 6-10 parts of azotobacter microbial inoculum and 10-20 parts of EM microbial inoculum.
In a preferred embodiment of the present invention, the treated agricultural wastewater comprises: COD is 30-40mg/L, BOC:60-90mg/L, TSS (total suspended solids): 30-60mg/L, TN (total Nitrogen): 100-150mg/L, ammonia nitrogen 30-60mg/L, pH7.0-6, 7.5, fecal coliform number 0.1-0.2 × 10 6 L, 1-2mg/L of organophosphorus pesticide and 0.3-0.8mg/L of dimethoate.
Advantageous effects
Aiming at the characteristics of agricultural sewage, the invention reasonably sets the compound microbial agent, and combines with chlorella HL and scenedesmus obliquus inoculation, so that pollutants in the agricultural sewage are pertinently removed, and the pollutants are recycled after quality inspection is qualified, thereby saving resources.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. It is to be understood that the described embodiments are merely exemplary of the invention, and not restrictive of the full scope of the invention. All other embodiments, which can be obtained by a person skilled in the art without making any creative effort based on the embodiments in the present invention, belong to the protection scope of the present invention.
Unless otherwise specified, the reagents involved in the examples of the present invention are all commercially available products and commercially available.
Example 1
In the initial detection of sewage: COD 824mg/L, BOC:892mg/L, TSS:660mg/L, TN:721mg/L, 435mg/L ammonia nitrogen, pH6.2, and 10 fecal coliform bacteria population 6 The concentration of the organophosphorus pesticide is 4.2mg/L, and the concentration of the dimethoate is 2.2mg/L. Inoculating Chlorella HL and Scenedesmus obliquus (Chlorella HL and Scenedesmus obliquus at a ratio of 1: 4) into the primary wastewater with an inoculation amount of 1 × 10 5 And culturing the strain per mL at 20 ℃, wherein the light-dark ratio is 11h and the illumination intensity is 1000lux for 3d, and adding 10 g/ton of compound microbial agent which is shown in CN108841767B example 1 and comprises the following components: bacillus subtilis XF-1 microbial inoculum (the content is 20 multiplied by 10) 8 cfu/g) 20 parts by weight of a trichoderma viride microbial inoculum (the content is 1 multiplied by 10) 8 cfu/g) 10 weight portions, bifidobacterium agent (content is 10 multiplied by 10) 8 cfu/g) 8 weight portions, yeast bacterial agent (content is 2X 10) 8 cfu/g) 8 parts by weight, and lactobacillus agent (content is 2 x 10) 8 cfu/g) 6 parts by weight, nitrobacteria agent (the content is 1 multiplied by 10) 8 cfu/g) 6 parts by weight, azotobacteria (content is 1 x 10) 8 cfu/g) 8 weight portions, EM bacterial agent (the content is 5 multiplied by 10) 8 cfu/g) 15 parts by weight; and (4) continuously culturing for 8 days, removing the microalgae, and measuring various indexes in the sewage, wherein the results are shown in a table 1.
Example 2:
the same as example 1, except that Chlorella HL and Scenedesmus obliquus (Chlorella HL and Scenedesmus obliquus in a ratio of 1: 2) were inoculated into the preliminary test wastewater at an inoculation amount of 1X 10 5 one/mL. After the treatment, various indexes in the sewage were measured, and the results are shown in table 1.
Example 3:
the same as example 1, except that 15 g/ton of the complex microbial agent was used. After the treatment, various indexes in the sewage were measured, and the results are shown in table 1.
Comparative example 1:
the same as example 1, except that the composite microbial agent only comprises bacillus subtilis XF-1 microbial agent (the content is 20 multiplied by 10) 8 cfu/g) 20 parts by weight of nitrobacteria agent (the content is 1 multiplied by 10) 8 cfu/g) 6 weight portions, azotobacteria (content is 1X 10) 8 cfu/g) 8 parts by weight. After the treatment, various indexes in the sewage were measured, and the results are shown in table 1.
Comparative example 2:
the same as example 1, except that the blue algae of brown algae were inoculated into the initial wastewater at an inoculation amount of 1X 10 5 one/mL. After the treatment, various indexes in the sewage were measured, and the results are shown in table 1.
TABLE 1 results of the Sewage treatment
Item Example 1 Example 2 Example 3 Comparative example 1 Comparative example 2
COD（mg/L） 38 33 34 128 210
BOC（mg/L） 72 70 75 178 189
TSS（mg/L） 45 38 39 118 195
TN（mg/L） 123 110 115 212 223
Ammonia nitrogen (mg/L) 39 32 34 124 145
pH 7.2 7.3 7.3 7.5 6.2
Faecal coliform population number 10 5 /L 1.5 1.4 1.6 2.1 2.3
Organophosphorus pesticide (mg/L) 1.3 1.4 1.5 1.8 1.6
Dimethoate (mg/L) 0.6 0.5 0.5 0.8 0.9
The above experimental results show that the treatment efficiency of agricultural sewage can be effectively improved by using the specific compound microbial inoculum in combination with the ginkgo polysaccharide in a specific ratio, wherein the treatment effect of the example 2 is the best.
The preferred embodiments of the present invention have been described above, but the present invention is not limited thereto. Modifications and variations of the embodiments disclosed herein may be made by those skilled in the art without departing from the scope and spirit of the invention.

Claims (8)

1. A method for treating agricultural sewage, which is characterized by comprising the following steps: inoculating chlorella HL and scenedesmus obliquus in agricultural sewage, culturing for 2-4 days, adding a compound microbial agent containing Bacillus subtilis XF-1, trichoderma viride, bifidobacterium, yeast, lactobacillus, nitrifying bacteria, azotobacter and EM, and continuously culturing for 6-10 days.
2. The method of claim 1, wherein the chlorella HL and scenedesmus obliquus are present in a ratio of 1:1-5.
3. The method of claim 1, wherein the chlorella HL and scenedesmus obliquus are present in a ratio of 1:1.5-2.5.
4. The method according to any one of claims 1-3, wherein the total amount of Chlorella HL and Scenedesmus obliquus is 0.8-1.5 x 10 5 Per mL of wastewater.
5. The method according to claim 1, wherein the amount of the complex microbial inoculum added is 8-20 g/ton of wastewater, preferably 10-15 g/ton of wastewater.
6. The method of claim 1, wherein the method is performed in a batch processThe agricultural sewage contains 800-900mg/L of COD, 800-1000mg/L of BOC, 600-800mg/L of total suspended solids, 700-800mg/L of total nitrogen, 400-800mg/L of ammonia nitrogen, 6.0-6.5 of pH value and 0.5-2 multiplied by 10 of faecal coliform group number 6 L, 3-5mg/L of organophosphorus pesticide and 1-4mg/L of dimethoate.
7. The method of claim 1, wherein the complex microbial inoculant comprises: 15-25 parts of bacillus subtilis XF-1 microbial inoculum, 8-12 parts of trichoderma viride microbial inoculum, 6-10 parts of bifidobacterium microbial inoculum, 6-10 parts of yeast microbial inoculum, 4-8 parts of lactobacillus microbial inoculum, 4-8 parts of nitrobacteria microbial inoculum, 6-10 parts of azotobacter microbial inoculum and 10-20 parts of EM microbial inoculum.
8. The method of claim 1, wherein in the treated agricultural wastewater: COD is 30-40mg/L, BOC is 60-90mg/L, total suspended solid is 30-60mg/L, total nitrogen is 100-150mg/L, ammonia nitrogen is 30-60mg/L, pH is 7.0-6, 7.5, fecal coliform number is 0.1-0.2 x 10 6 L, 1-2mg/L of organophosphorus pesticide and 0.3-0.8mg/L of dimethoate.
CN202211079653.4A 2022-09-05 2022-09-05 Agricultural sewage treatment method Pending CN115385457A (en)

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Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101724596A (en) * 2010-01-11 2010-06-09 北京联合大学 Method for culturing organophosphorus pesticide degrading bacteria
CN104087544A (en) * 2014-06-19 2014-10-08 徐州工程学院 Engineering bacterium capable of degrading organophosphorus pesticides, and construction method and application thereof
CN106336304A (en) * 2016-08-26 2017-01-18 中国科学院上海高等研究院 Bacteria-alga complexing agent used for soil improvement and directional disease and insect pest prevention and preparation method thereof
CN107324504A (en) * 2017-08-09 2017-11-07 中国科学院水生生物研究所 A kind of compound algae microbial inoculum improved for culture-pool water quality and preparation method thereof
CN108793432A (en) * 2018-07-04 2018-11-13 南京怡可帮生态环境科技有限公司 A kind of underground water organophosphor chlorine sulphur pollution by pesticides bioremediation technology and application
CN108841767A (en) * 2018-09-21 2018-11-20 云南星耀生物制品有限公司 A kind of complex micro organism fungicide and microbial-bacterial fertilizer
CN109133355A (en) * 2018-08-22 2019-01-04 厦门水世纪环保科技有限公司 A kind of high nitrogen phosphorus creek water remediation method and immobilization cell
CN109368801A (en) * 2018-11-23 2019-02-22 江苏大学 A method of the light based on microalgae helps Fenton-like system to handle organic pollutants in water body
CN109574235A (en) * 2018-12-25 2019-04-05 武汉理工大学 Utilize microalgae while the method for Removal of Phosphorus in Wastewater and mercury-containing waste water

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101724596A (en) * 2010-01-11 2010-06-09 北京联合大学 Method for culturing organophosphorus pesticide degrading bacteria
CN104087544A (en) * 2014-06-19 2014-10-08 徐州工程学院 Engineering bacterium capable of degrading organophosphorus pesticides, and construction method and application thereof
CN106336304A (en) * 2016-08-26 2017-01-18 中国科学院上海高等研究院 Bacteria-alga complexing agent used for soil improvement and directional disease and insect pest prevention and preparation method thereof
CN107324504A (en) * 2017-08-09 2017-11-07 中国科学院水生生物研究所 A kind of compound algae microbial inoculum improved for culture-pool water quality and preparation method thereof
CN108793432A (en) * 2018-07-04 2018-11-13 南京怡可帮生态环境科技有限公司 A kind of underground water organophosphor chlorine sulphur pollution by pesticides bioremediation technology and application
CN109133355A (en) * 2018-08-22 2019-01-04 厦门水世纪环保科技有限公司 A kind of high nitrogen phosphorus creek water remediation method and immobilization cell
CN108841767A (en) * 2018-09-21 2018-11-20 云南星耀生物制品有限公司 A kind of complex micro organism fungicide and microbial-bacterial fertilizer
CN109368801A (en) * 2018-11-23 2019-02-22 江苏大学 A method of the light based on microalgae helps Fenton-like system to handle organic pollutants in water body
CN109574235A (en) * 2018-12-25 2019-04-05 武汉理工大学 Utilize microalgae while the method for Removal of Phosphorus in Wastewater and mercury-containing waste water

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Title
环境保护部自然生态保护司编: "《舌尖上的安全 食品中的有毒有害物质警示》", 广州中山大学出版社, pages: 138 - 281 *

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