CN115381728B - Anti-aging and permeation-promoting composition containing adenosine and application thereof - Google Patents
Anti-aging and permeation-promoting composition containing adenosine and application thereof Download PDFInfo
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- CN115381728B CN115381728B CN202211017221.0A CN202211017221A CN115381728B CN 115381728 B CN115381728 B CN 115381728B CN 202211017221 A CN202211017221 A CN 202211017221A CN 115381728 B CN115381728 B CN 115381728B
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- OIRDTQYFTABQOQ-KQYNXXCUSA-N adenosine Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O OIRDTQYFTABQOQ-KQYNXXCUSA-N 0.000 title claims abstract description 356
- 239000002126 C01EB10 - Adenosine Substances 0.000 title claims abstract description 176
- 229960005305 adenosine Drugs 0.000 title claims abstract description 176
- 230000003712 anti-aging effect Effects 0.000 title claims abstract description 113
- 239000000203 mixture Substances 0.000 title claims abstract description 77
- 239000002537 cosmetic Substances 0.000 claims abstract description 14
- 239000006071 cream Substances 0.000 claims description 31
- 238000003756 stirring Methods 0.000 claims description 23
- 230000001804 emulsifying effect Effects 0.000 claims description 20
- 239000012071 phase Substances 0.000 claims description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 18
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 15
- 238000001816 cooling Methods 0.000 claims description 15
- 238000000034 method Methods 0.000 claims description 15
- 238000005086 pumping Methods 0.000 claims description 15
- VBICKXHEKHSIBG-UHFFFAOYSA-N 1-monostearoylglycerol Chemical compound CCCCCCCCCCCCCCCCCC(=O)OCC(O)CO VBICKXHEKHSIBG-UHFFFAOYSA-N 0.000 claims description 10
- TXFPEBPIARQUIG-UHFFFAOYSA-N 4'-hydroxyacetophenone Chemical compound CC(=O)C1=CC=C(O)C=C1 TXFPEBPIARQUIG-UHFFFAOYSA-N 0.000 claims description 10
- NOPFSRXAKWQILS-UHFFFAOYSA-N docosan-1-ol Chemical compound CCCCCCCCCCCCCCCCCCCCCCO NOPFSRXAKWQILS-UHFFFAOYSA-N 0.000 claims description 10
- YPFDHNVEDLHUCE-UHFFFAOYSA-N propane-1,3-diol Chemical compound OCCCO YPFDHNVEDLHUCE-UHFFFAOYSA-N 0.000 claims description 10
- 230000001815 facial effect Effects 0.000 claims description 8
- 238000010438 heat treatment Methods 0.000 claims description 8
- -1 polydimethylsiloxane Polymers 0.000 claims description 8
- QRYRORQUOLYVBU-VBKZILBWSA-N Carnosic acid Natural products CC([C@@H]1CC2)(C)CCC[C@]1(C(O)=O)C1=C2C=C(C(C)C)C(O)=C1O QRYRORQUOLYVBU-VBKZILBWSA-N 0.000 claims description 7
- 108010087806 Carnosine Proteins 0.000 claims description 7
- BIVBRWYINDPWKA-VLQRKCJKSA-L Glycyrrhizinate dipotassium Chemical compound [K+].[K+].O([C@@H]1[C@@H](O)[C@H](O)[C@H](O[C@@H]1O[C@H]1CC[C@]2(C)[C@H]3C(=O)C=C4[C@@H]5C[C@](C)(CC[C@@]5(CC[C@@]4(C)[C@]3(C)CC[C@H]2C1(C)C)C)C(O)=O)C([O-])=O)[C@@H]1O[C@H](C([O-])=O)[C@@H](O)[C@H](O)[C@H]1O BIVBRWYINDPWKA-VLQRKCJKSA-L 0.000 claims description 7
- CQOVPNPJLQNMDC-UHFFFAOYSA-N N-beta-alanyl-L-histidine Natural products NCCC(=O)NC(C(O)=O)CC1=CN=CN1 CQOVPNPJLQNMDC-UHFFFAOYSA-N 0.000 claims description 7
- 229940044199 carnosine Drugs 0.000 claims description 7
- CQOVPNPJLQNMDC-ZETCQYMHSA-N carnosine Chemical compound [NH3+]CCC(=O)N[C@H](C([O-])=O)CC1=CNC=N1 CQOVPNPJLQNMDC-ZETCQYMHSA-N 0.000 claims description 7
- 108050003126 conotoxin Proteins 0.000 claims description 7
- 229940101029 dipotassium glycyrrhizinate Drugs 0.000 claims description 7
- LXAHHHIGZXPRKQ-UHFFFAOYSA-N 5-fluoro-2-methylpyridine Chemical compound CC1=CC=C(F)C=N1 LXAHHHIGZXPRKQ-UHFFFAOYSA-N 0.000 claims description 5
- NIXOWILDQLNWCW-UHFFFAOYSA-N Acrylic acid Chemical compound OC(=O)C=C NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 claims description 5
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims description 5
- 229920002125 Sokalan® Polymers 0.000 claims description 5
- 229960001631 carbomer Drugs 0.000 claims description 5
- GHVNFZFCNZKVNT-UHFFFAOYSA-M decanoate Chemical compound CCCCCCCCCC([O-])=O GHVNFZFCNZKVNT-UHFFFAOYSA-M 0.000 claims description 5
- 239000004205 dimethyl polysiloxane Substances 0.000 claims description 5
- 229960000735 docosanol Drugs 0.000 claims description 5
- 238000000855 fermentation Methods 0.000 claims description 5
- 230000004151 fermentation Effects 0.000 claims description 5
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 claims description 5
- 229910052708 sodium Inorganic materials 0.000 claims description 5
- 239000011734 sodium Substances 0.000 claims description 5
- 241000589596 Thermus Species 0.000 claims description 4
- 238000000265 homogenisation Methods 0.000 claims description 4
- 238000002156 mixing Methods 0.000 claims description 4
- 239000002994 raw material Substances 0.000 claims description 4
- 238000005303 weighing Methods 0.000 claims description 4
- HFJHNGKIVAKCIW-UHFFFAOYSA-N Stearyl monoglyceridyl citrate Chemical compound OCC(O)CO.OC(=O)CC(O)(CC(O)=O)CC(O)=O.CCCCCCCCCCCCCCCCCC(O)=O HFJHNGKIVAKCIW-UHFFFAOYSA-N 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims description 3
- 239000008346 aqueous phase Substances 0.000 claims description 2
- 239000008367 deionised water Substances 0.000 claims description 2
- 229910021641 deionized water Inorganic materials 0.000 claims description 2
- JEGUKCSWCFPDGT-UHFFFAOYSA-N h2o hydrate Chemical compound O.O JEGUKCSWCFPDGT-UHFFFAOYSA-N 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 22
- 230000037394 skin elasticity Effects 0.000 abstract description 6
- 230000002195 synergetic effect Effects 0.000 abstract description 5
- 206010051246 Photodermatosis Diseases 0.000 abstract description 2
- 230000008845 photoaging Effects 0.000 abstract description 2
- 231100000331 toxic Toxicity 0.000 abstract description 2
- 230000002588 toxic effect Effects 0.000 abstract description 2
- 238000012360 testing method Methods 0.000 description 23
- ZKHQWZAMYRWXGA-KQYNXXCUSA-J ATP(4-) Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)[C@H]1O ZKHQWZAMYRWXGA-KQYNXXCUSA-J 0.000 description 21
- ZKHQWZAMYRWXGA-UHFFFAOYSA-N Adenosine triphosphate Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)C(O)C1O ZKHQWZAMYRWXGA-UHFFFAOYSA-N 0.000 description 21
- 239000003642 reactive oxygen metabolite Substances 0.000 description 18
- 210000002950 fibroblast Anatomy 0.000 description 16
- WCVRQHFDJLLWFE-UHFFFAOYSA-N pentane-1,2-diol Chemical compound CCCC(O)CO WCVRQHFDJLLWFE-UHFFFAOYSA-N 0.000 description 16
- AXTGDCSMTYGJND-UHFFFAOYSA-N 1-dodecylazepan-2-one Chemical compound CCCCCCCCCCCCN1CCCCCC1=O AXTGDCSMTYGJND-UHFFFAOYSA-N 0.000 description 14
- 210000004027 cell Anatomy 0.000 description 14
- 230000005764 inhibitory process Effects 0.000 description 10
- MTHSVFCYNBDYFN-UHFFFAOYSA-N diethylene glycol Chemical compound OCCOCCO MTHSVFCYNBDYFN-UHFFFAOYSA-N 0.000 description 9
- 239000000126 substance Substances 0.000 description 9
- 229910002091 carbon monoxide Inorganic materials 0.000 description 8
- 230000002708 enhancing effect Effects 0.000 description 8
- 239000013642 negative control Substances 0.000 description 8
- 230000035515 penetration Effects 0.000 description 8
- 230000000052 comparative effect Effects 0.000 description 7
- 230000003698 anagen phase Effects 0.000 description 6
- 230000001153 anti-wrinkle effect Effects 0.000 description 6
- 238000012258 culturing Methods 0.000 description 6
- 238000001514 detection method Methods 0.000 description 6
- 229920006284 nylon film Polymers 0.000 description 6
- 230000002407 ATP formation Effects 0.000 description 5
- 230000035699 permeability Effects 0.000 description 5
- 238000010521 absorption reaction Methods 0.000 description 4
- 239000003623 enhancer Substances 0.000 description 4
- 210000002615 epidermis Anatomy 0.000 description 4
- 238000012986 modification Methods 0.000 description 4
- 230000004048 modification Effects 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- 238000003860 storage Methods 0.000 description 4
- RYHBNJHYFVUHQT-UHFFFAOYSA-N 1,4-Dioxane Chemical compound C1COCCO1 RYHBNJHYFVUHQT-UHFFFAOYSA-N 0.000 description 3
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 description 3
- 238000009825 accumulation Methods 0.000 description 3
- 230000009471 action Effects 0.000 description 3
- 229910052785 arsenic Inorganic materials 0.000 description 3
- RQNWIZPPADIBDY-UHFFFAOYSA-N arsenic atom Chemical compound [As] RQNWIZPPADIBDY-UHFFFAOYSA-N 0.000 description 3
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 3
- 229910052793 cadmium Inorganic materials 0.000 description 3
- BDOSMKKIYDKNTQ-UHFFFAOYSA-N cadmium atom Chemical compound [Cd] BDOSMKKIYDKNTQ-UHFFFAOYSA-N 0.000 description 3
- 238000000684 flow cytometry Methods 0.000 description 3
- 239000001963 growth medium Substances 0.000 description 3
- 229910001385 heavy metal Inorganic materials 0.000 description 3
- 238000011534 incubation Methods 0.000 description 3
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 description 3
- 229910052753 mercury Inorganic materials 0.000 description 3
- 239000001301 oxygen Substances 0.000 description 3
- 229910052760 oxygen Inorganic materials 0.000 description 3
- 239000003755 preservative agent Substances 0.000 description 3
- 230000002335 preservative effect Effects 0.000 description 3
- 230000001737 promoting effect Effects 0.000 description 3
- 210000003491 skin Anatomy 0.000 description 3
- 238000013112 stability test Methods 0.000 description 3
- 230000032683 aging Effects 0.000 description 2
- PYMYPHUHKUWMLA-LMVFSUKVSA-N aldehydo-D-ribose Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)C=O PYMYPHUHKUWMLA-LMVFSUKVSA-N 0.000 description 2
- 238000004113 cell culture Methods 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 238000010835 comparative analysis Methods 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 238000011076 safety test Methods 0.000 description 2
- 238000007619 statistical method Methods 0.000 description 2
- 230000000638 stimulation Effects 0.000 description 2
- 210000000434 stratum corneum Anatomy 0.000 description 2
- 229930024421 Adenine Natural products 0.000 description 1
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 1
- 102000003844 DNA helicases Human genes 0.000 description 1
- 108090000133 DNA helicases Proteins 0.000 description 1
- 230000006820 DNA synthesis Effects 0.000 description 1
- 206010020751 Hypersensitivity Diseases 0.000 description 1
- 238000000692 Student's t-test Methods 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 229960000643 adenine Drugs 0.000 description 1
- 230000007815 allergy Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000000845 anti-microbial effect Effects 0.000 description 1
- OIRDTQYFTABQOQ-UHFFFAOYSA-N ara-adenosine Natural products Nc1ncnc2n(cnc12)C1OC(CO)C(O)C1O OIRDTQYFTABQOQ-UHFFFAOYSA-N 0.000 description 1
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000004663 cell proliferation Effects 0.000 description 1
- 230000001186 cumulative effect Effects 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 230000037149 energy metabolism Effects 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 210000003495 flagella Anatomy 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 239000006210 lotion Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 210000003470 mitochondria Anatomy 0.000 description 1
- 230000004065 mitochondrial dysfunction Effects 0.000 description 1
- 230000003020 moisturizing effect Effects 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 230000004792 oxidative damage Effects 0.000 description 1
- 230000007903 penetration ability Effects 0.000 description 1
- 239000003961 penetration enhancing agent Substances 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 238000001243 protein synthesis Methods 0.000 description 1
- 238000004451 qualitative analysis Methods 0.000 description 1
- 150000003254 radicals Chemical class 0.000 description 1
- 230000006950 reactive oxygen species formation Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000002829 reductive effect Effects 0.000 description 1
- 230000037384 skin absorption Effects 0.000 description 1
- 231100000274 skin absorption Toxicity 0.000 description 1
- 230000009759 skin aging Effects 0.000 description 1
- 208000017520 skin disease Diseases 0.000 description 1
- 210000001626 skin fibroblast Anatomy 0.000 description 1
- 230000037393 skin firmness Effects 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 238000012353 t test Methods 0.000 description 1
- 238000010257 thawing Methods 0.000 description 1
- 230000014616 translation Effects 0.000 description 1
- 230000037303 wrinkles Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/60—Sugars; Derivatives thereof
- A61K8/606—Nucleosides; Nucleotides; Nucleic acids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/49—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
- A61K8/494—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with more than one nitrogen as the only hetero atom
- A61K8/4946—Imidazoles or their condensed derivatives, e.g. benzimidazoles
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/63—Steroids; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/99—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from microorganisms other than algae or fungi, e.g. protozoa or bacteria
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Dermatology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Gerontology & Geriatric Medicine (AREA)
- Chemical & Material Sciences (AREA)
- Cosmetics (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention discloses an adenosine-containing anti-aging and penetration-promoting composition and application thereof in cosmetics. The weight ratio of the adenosine to the molecular motor is 1:5-15. The invention combines the adenosine with the molecular motor, promotes the permeation of the adenosine in the skin, solves the problem of low bioavailability of the adenosine in the body, and can reduce the concentration of the adenosine which is singly used, thereby reducing the toxic and side effects of the adenosine which is singly used. The composition of the adenosine and the molecular motor has better anti-aging effect than the adenosine and the molecular motor which are singly used, and shows the synergistic effect of improving the photoaging of the skin. In addition, the permeation-promoting composition containing the adenosine is combined with a plurality of anti-aging components to prepare the adenosine permeation-promoting anti-aging product, and the skin elasticity and the skin compactness can be effectively improved.
Description
Technical Field
The invention belongs to the field of cosmetics, and in particular relates to an adenosine-containing anti-aging and penetration-promoting composition and application thereof in cosmetics.
Background
Adenosine, also known as adenine nucleoside, is a compound formed by connecting N-9 of adenine and C-1 of D-ribose through beta glycosidic bond, and is a water-soluble small molecule. It has been shown that adenosine significantly promotes fibroblast DNA and protein synthesis, increases skin epidermis thickness, and reduces periocular and glabellar wrinkles. However, the half-life and the action time of adenosine in vivo are short, and the hydrophobicity of the stratum corneum results in relatively poor permeability of hydrophilic substances, resulting in insignificant action of adenosine.
Disclosure of Invention
In order to overcome the technical problems, the invention provides an anti-aging and permeation-promoting composition containing adenosine, a facial cream and application thereof.
In order to achieve the above object, the technical scheme provided by the invention is as follows:
an anti-aging and permeation-promoting composition containing adenosine comprises 1-15 parts by weight of adenosine and 80-99 parts by weight of molecular motor.
Further, the composition comprises 5 to 10 parts by weight of adenosine and 80 to 90 parts by weight of molecular motor.
Further, the weight portion of the molecular motor of the anti-aging and permeation-promoting composition containing the adenosine is 5-15 times of that of the adenosine.
Further, the adenosine-containing anti-aging and penetration-promoting composition is characterized in that: the composition comprises 1 part by weight of adenosine and 8 parts by weight of molecular motor.
The molecular motor refers to thermophilic thermus fermentation liquid.
The application of the adenosine-containing anti-aging and penetration-promoting composition in preparing anti-aging cosmetics adopts any adenosine-containing anti-aging and penetration-promoting composition.
The application of the adenosine-containing anti-aging and penetration-promoting composition in preparing anti-aging cosmetics comprises the step of adding the anti-aging and penetration-promoting composition into anti-aging essence or cream cosmetics according to the addition amount of 1-20% by mass.
The adenosine anti-aging and permeation-promoting facial cream comprises 0.1-8 parts by weight of the adenosine-containing anti-aging and permeation-promoting composition, 1-5 parts by weight of conotoxin, 0.1-0.5 part by weight of carnosine, 0.1-0.5 part by weight of dipotassium glycyrrhizinate and 50-70 parts by weight of water, wherein the water is preferably deionized water.
The preparation method of the adenosine permeation-promoting anti-aging face cream comprises the following steps:
s1: weighing the raw materials according to the component proportion of the anti-aging and permeation-promoting composition containing the adenosine, and uniformly mixing to obtain the anti-aging and permeation-promoting composition containing the adenosine;
s2: adding 0.1-10 parts by weight of glycerol stearate, 0.5-10 parts by weight of cocoyl alcohol-caprylate/caprate, 0.5-20 parts by weight of polydimethylsiloxane, 0.5-20 parts by weight of behenyl alcohol and 0.5-20 parts by weight of glycerol stearate citrate into an oil phase pot, heating to 60-90 ℃, stirring and dissolving for standby;
s3: adding 30-100 parts by weight of water, 0.5-20 parts by weight of 1, 3-propylene glycol, 0.5-20 parts by weight of glycerol, 0.05-3 parts by weight of dipotassium glycyrrhizinate, 0.1-20 parts by weight of p-hydroxyacetophenone, 0.01-5 parts by weight of EDTA disodium and 0.01-20 parts by weight of an adenosine-containing anti-aging and permeation-promoting composition into a water phase pot, heating to 60-90 ℃, stirring and dissolving for standby;
s4: pumping the oil phase pot into the emulsifying pot, stirring for 15-35rpm/min, opening homogenizing for 1500-3500rpm/min, pumping the water phase into the emulsifying pot, maintaining the homogenization for 3-20min after the pumping, defoaming, and cooling to 30-60deg.C;
s5: adding 0.05-3 parts by weight of carbomer sodium into an emulsifying pot, stirring for 10-45rpm/min, homogenizing for 1000-3500rpm/min, and keeping for 2-8min. Defoaming and cooling to 20-55 ℃;
s6: adding 0.01-3 parts by weight of carnosine and 0.05-15 parts by weight of conotoxin into an emulsifying pot, stirring at 10-45rpm/min, homogenizing at 1000-3500rpm/min, defoaming, cooling to 20-50deg.C, and making into adenosine permeation-promoting antiaging cream.
Further, the preparation method of the adenosine permeation-promoting anti-aging face cream comprises the following steps:
s1: weighing the raw materials according to the component proportion of the anti-aging and permeation-promoting composition containing the adenosine, and uniformly mixing to obtain the anti-aging and permeation-promoting composition containing the adenosine;
s2: adding 0.5-5 parts by weight of glycerol stearate, 1-5 parts by weight of cocoyl-caprylate/caprate, 1-10 parts by weight of polydimethylsiloxane, 1-10 parts by weight of behenyl alcohol and 1-10 parts by weight of glycerol stearate citric acid ester into an oil phase pot, heating to 80 ℃, stirring and dissolving for standby;
s3: adding 50-70 parts by weight of water, 1-10 parts by weight of 1, 3-propylene glycol, 1-5 parts by weight of glycerol, 0.1-0.5 part by weight of dipotassium glycyrrhizinate, 0.4-1 part by weight of p-hydroxyacetophenone, 0.05-0.1 part by weight of EDTA disodium and 0.1-8 parts by weight of an adenosine-containing anti-aging and permeation-promoting composition into an aqueous phase pot, heating to 80 ℃, stirring and dissolving for standby;
s4: pumping the oil phase pot into the emulsifying pot, stirring for 20-25rpm/min, homogenizing for 2000-2500rpm/min, pumping the water phase into the emulsifying pot, homogenizing for 5-10min after pumping, defoaming, and cooling to 50deg.C;
s5: adding 0.1-1 weight part of carbomer sodium into the emulsifying pot, stirring for 20-25rpm/min, and homogenizing for 2000-2500rpm/min. Homogenizing for 3-5min. Defoaming and cooling to 40 ℃;
s6: adding 0.1-0.5 weight part of carnosine and 1-5 weight parts of conotoxin into an emulsifying pot, stirring at 20-25rpm/min, homogenizing at 2000-2500rpm/min, defoaming, cooling to 35 ℃, and obtaining the adenosine permeation-promoting anti-aging facial cream.
The invention overcomes the technical problems that the half-life period of the adenosine in the body and the hydrophobic property of the upper stratum corneum with short acting time cause relatively poor permeability of hydrophilic substances and cause the action effect of the adenosine to be not obvious, and the composition, the preparation and the administration mode of the invention are selected to improve the bioavailability of the adenosine. The invention innovatively increases the penetration enhancer, which is used for increasing the permeability of the adenosine and increasing the percutaneous absorption rate of the adenosine.
Molecular motors are a protein that is widely present in cells, and the most important functions are as a carrier for substance transport, including ATP synthase, flagellum motor, and DNA helicase, etc. The thermophilic thermus fermentation liquid is prepared by high temperature fermentation and contains a molecular motor. Molecular motors are capable of generating ATP to provide the energy required for skin absorption and metabolism, while the natural affinity of vesicles can also increase the efficiency of transdermal absorption of active substances. The composition can effectively exert the anti-aging effect of the adenosine, improves the effect application of the adenosine in cosmetics, firstly selects an adenosine compound molecular motor, optimizes the compound proportion of the adenosine and the molecular motor, can improve the transdermal absorption of the adenosine, and exerts the effects of resisting aging and wrinkling to the greatest extent.
The anti-aging and permeation-promoting composition containing the adenosine effectively solves the problems of poor solubility, poor permeability and the like of the adenosine applied to cosmetics, improves the bioavailability of the adenosine, and can reduce the concentration of the adenosine used independently, thereby reducing the toxic and side effects of the adenosine used independently.
The anti-aging and permeation-promoting composition containing the adenosine contains two anti-aging components of the adenosine and a molecular motor, can synergistically improve skin photoaging, and has better effect than the single use of the adenosine or the molecular motor.
The adenosine-containing permeation-promoting composition is combined with a plurality of anti-aging components, and the prepared adenosine permeation-promoting anti-aging product can effectively improve skin elasticity and compactness, and has better anti-aging and tightening effects.
Description of the drawings:
fig. 1: summarizing the transdermal fluorescence pictures of an adenosine-containing anti-aging and permeation promoting composition and adenosine;
fig. 2: the anti-aging and penetration-promoting composition of the adenosine and the fluorescence intensity statistics of the adenosine model slice at different time points;
fig. 3: effects of an adenosine-containing anti-aging and penetration enhancing composition, adenosine, and molecular motor on the ATP content of fibroblasts;
fig. 4: effects of adenosine-containing permeation-promoting compositions and adenosine, molecular motors on fibroblast ROS levels.
Detailed Description
The following experimental examples and examples serve to further illustrate but not limit the invention.
The following description of the embodiments of the present invention, taken in conjunction with the accompanying drawings, will provide a clear and complete description of the embodiments of the present invention, and it is evident that the embodiments described are only some, but not all, embodiments of the present invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention. The thermophilic thermus fermentation broth can be prepared by itself, can be purchased from the market, and can be purchased from Hangzhou Youma biotechnology limited company, batch number 20220427.
EXAMPLE 1 comparison of the transdermal Effect of adenosine-containing anti-aging and permeation-promoting compositions and adenosine
An anti-aging and permeation-promoting composition containing adenosine comprises 1 part by weight and 8 parts by weight of adenosine and a molecular motor. To better illustrate the transdermal permeation enhancing effect of adenosine, the anti-aging permeation enhancing composition prepared by the invention is used for carrying out comparative evaluation on permeation effect with adenosine. Qualitative analysis of the penetration ability of the test samples was performed based on a 3D epidermal skin model. Firstly, carrying out fluorescent marking on adenosine of an object to be detected by using Fluorescein Isothiocyanate (FITC) to form a FITC-adenosine conjugate, then uniformly smearing the marked sample on a model indication by adopting an indication administration mode, detecting the fluorescence intensity of the sample in the model at different time points, and evaluating the permeability of the sample to be detected according to the distribution of fluorescence signals.
Modeling the 3D epidermis skinLot number: ES220607, guangdong Boxi Biotechnology Co., ltd.) was transferred to a 6-well plate (0.9 mL of model culture medium was added in advance), and the 6-well plate was labeled with the test group number. Taking 25 mu L of fluorescent marked test sample, adding the test sample on the surface of the model, clamping nylon films by forceps, covering the nylon films on the surface of each model, uniformly distributing the test sample on the surface of the model, and placing the test sample on CO 2 Incubator (37 ℃, 5% CO) 2 ) Respectively, 0.5h, 8h and 24h. After the end of the dosing incubation, the model was fixed and frozen and the sections were fluorescent photographed. The result of fluorescence photographing is shown in FIG. 1, and the fluorescence intensity data is shown in FIG. 2. The comparison between the two groups uses t-test statistical analysis, all of which are double-tailed. P < 0.05 was considered to be of differential significance, with the smaller the P value, the more significant the differential. # represents significance analysis between samples, x represents P<0.05 represents P<0.01. From the results, it was found that the accumulation amount of the fluorescent substance at any one of the time points of 0.5h, 8h and 24h was significantly higher than that at the previous time point (P<0.01 Adenosine is capable of percutaneous absorption. However, compared with the adenosine group, the accumulation amount of fluorescent substances at 8h and 24h is significantly higher for the adenosine and molecular motor group than for the adenosine group at the same time, showing that the transdermal penetration amount and bioavailability of adenosine can be significantly improved by the adenosine and molecular motor combination.
EXAMPLE 2 comparison of anti-aging and permeation-promoting compositions containing adenosine with anti-wrinkle effects of adenosine, molecular motors
An anti-aging and permeation-promoting composition containing adenosine comprises 1 part by weight and 8 parts by weight of adenosine and a molecular motor. To better illustrate the efficacy of the invention, the anti-aging and permeation-promoting composition prepared by the invention is used for carrying out the comparison evaluation of the anti-wrinkle effect with adenosine and a molecular motor. Mitochondria are an important source of Adenosine Triphosphate (ATP), and also an important site for Reactive Oxygen Species (ROS), and mitochondrial dysfunction is closely related to skin aging. It was found that an imbalance in energy metabolism of senescent skin fibroblasts is associated with a reduced level of ATP required for nucleotide biosynthesis and cell proliferation. The anti-wrinkle efficacy of the test samples was evaluated by their effect on the ATP content of the fibroblasts. Therefore, the influence of the adenosine-containing anti-aging and permeation-promoting composition, the adenosine and the molecular motor on the ATP content of the fibroblast is detected by using a kit method, and the anti-wrinkle effects of the adenosine-containing anti-aging and permeation-promoting composition, the adenosine and the molecular motor are examined.
Collecting fibroblast in logarithmic growth phase according to 4×10 4 Cells/well were seeded into 24-well plates. In incubator (37 ℃, 5% CO) 2 ) Is cultured for 24 hours. Drugs were added to each group of cell culture wells as described below in table 1, with 3 replicate wells per group. Culturing in incubator for 24 hr, and performing 30J/cm on the group with UVA irradiation 2 Is placed in an incubator for continuous cultivation for 24 hours. The ATP content of each cell group was measured by performing the procedure according to the instructions for the ATP kit.
TABLE 1 ATP laboratory grouping information
The anti-wrinkle results of the adenosine-containing anti-aging and permeation-promoting composition, adenosine, and molecular motor are shown in fig. 3. Compared with a negative control group, adenosine can obviously improve the reduction of ATP (P < 0.01) production caused by UVA irradiation at the concentration of 0.0313mg/mL, and the improvement rate is 10.85%; the increase rate of the molecular motor to the ATP content at 0.02504 percent concentration is 6.23 percent (P < 0.01); the ATP content of the anti-aging and permeation-promoting composition containing adenosine is improved by 16.45 percent (P is less than 0.01), which is obviously superior to that of the ATP content of single adenosine or a molecular motor. It can be seen that the adenosine-containing anti-aging and penetration-promoting composition shows a synergistic effect in increasing the decrease in ATP content of fibroblasts caused by UVA.
EXAMPLE 3 comparison of the anti-aging Effect of adenosine-containing anti-aging and permeation-promoting compositions, adenosine and molecular motors
An anti-aging and permeation-promoting composition containing adenosine comprises 1 part by weight and 8 parts by weight of adenosine and a molecular motor. In order to better illustrate the efficacy of the invention, the anti-aging and permeation-promoting composition prepared by the invention is used for carrying out the comparative evaluation of the anti-aging effect with adenosine and a molecular motor. ROS is a major factor in oxidative damage to cells, while the free radical theory supports the accumulation of ROS in humans as an important cause of aging. Excessive ROS in cells attack macromolecular proteins, active polysaccharides, nucleic acids and other substances, so that the cells are permanently damaged, and finally the cells are aged. Therefore, the influence of the adenosine-containing anti-aging and penetration-promoting composition, the adenosine and the molecular motor on the ROS level of fibroblasts is detected by using a kit method, and the anti-aging effects of the adenosine-containing anti-aging and penetration-promoting composition, the adenosine and the molecular motor are examined.
Collecting fibroblast in logarithmic growth phase according to 4×10 4 Cells/well were seeded into 24-well plates. In incubator (37 ℃, 5% CO) 2 ) Is cultured for 24 hours. Drugs were added to each group of cell culture wells as described below in table 2, with 3 replicate wells per group. Culturing in incubator for 24 hr, and performing 30J/cm on the group with UVA irradiation 2 Is placed in an incubator for continuous cultivation for 24 hours. The operation is carried out according to the instruction of the active oxygen kit, and the flow cytometry is used for machine detection.
TABLE 2 ROS experimental grouping information
The results of the anti-aging and permeation-promoting composition containing adenosine and the anti-aging of the adenosine, molecular motor are shown in FIG. 3. Compared with a negative control group, the adenosine has remarkable inhibition effect (p is less than 0.01) on ROS generated by UVA stimulation at the concentration of 0.0313mg/mL, and the inhibition rate is 35.75%; the inhibition rate of the molecular motor to the ROS content at the concentration of 0.02504% is 14.81% (P < 0.01); the inhibition rate of the anti-aging and penetration-promoting composition containing adenosine to the ROS content is 52.00% (P < 0.01). It can be seen that the anti-aging and penetration-promoting composition containing adenosine exhibits a synergistic effect in inhibiting the production of ROS by UVA-stimulated fibroblasts, significantly better than the use of adenosine alone and a molecular motor.
Comparative example 1
The procedure of example 1 was followed, except that the molecular motor in the adenosine-containing anti-aging and penetration enhancing composition was replaced with an equivalent portion of 1, 2-pentanediol. Modeling the 3D epidermis skinLot number: ES220607, guangdong Boxi Biotechnology Co., ltd.) was transferred to 6-well plates (0.9 mL of model culture medium was added in advance), randomly divided into a blank control group, a negative control group, an adenosine group, adenosine+1, 2-pentanediol, and test group numbers were marked on the 6-well plates. Taking 25 mu L of fluorescent marked test sample, adding the test sample on the surface of the model, clamping nylon films by forceps, covering the nylon films on the surface of each model, uniformly distributing the test sample on the surface of the model, and placing the test sample on CO 2 Incubator (37 ℃, 5% CO) 2 ) And incubated for 24h. After the end of the dosing incubation, the model was fixed and frozen and the sections were fluorescent photographed.
Comparative example 2
The procedure of example 2 was followed, except that the molecular motor in the adenosine-containing anti-aging and penetration enhancing composition was replaced with an equivalent portion of 1, 2-pentanediol. Collecting fibroblast in logarithmic growth phase according to 4×10 4 Cells/well were seeded into 24-well plates. In incubator (37 ℃, 5% CO) 2 ) Is cultured for 24 hours. Randomly, a blank control group, a negative control group, an adenosine group, and adenosine +1, 2-pentanediol, each group was provided with 3 duplicate wells. Culturing in incubator for 24 hr, and performing 30J/cm on the group with UVA irradiation 2 Is placed in an incubator for continuous cultivation for 24 hours. Advances according to the instructions for using the ATP kitThe column was run to measure the ATP content in each cell group.
Comparative example 3
The procedure of example 3 was followed, except that the molecular motor was replaced with an equivalent portion of 1, 2-pentanediol. Collecting fibroblast in logarithmic growth phase according to 4×10 4 Cells/well were seeded into 24-well plates. In incubator (37 ℃, 5% CO) 2 ) Is cultured for 24 hours. Randomly, a blank control group, a negative control group, an adenosine group, and adenosine +1, 2-pentanediol, each group was provided with 3 duplicate wells. Culturing in incubator for 24 hr, and performing 30J/cm on the group with UVA irradiation 2 Is placed in an incubator for continuous cultivation for 24 hours. The operation is carried out according to the instruction of the active oxygen kit, and the flow cytometry is used for machine detection.
Comparative example 4
The procedure of example 1 was followed, except that the molecular motor in the adenosine-containing anti-aging and penetration enhancing composition was replaced with an equivalent portion of azone. Modeling the 3D epidermis skinLot number: ES220607, guangdong Boxi Biotechnology Co., ltd.) was transferred to 6-well plates (0.9 mL of model culture medium was added in advance), randomly divided into a blank control group, a negative control group, an adenosine group, adenosine+azone, and test group numbers were labeled on the 6-well plates. Taking 25 mu L of fluorescent marked test sample, adding the test sample on the surface of the model, clamping nylon films by forceps, covering the nylon films on the surface of each model, uniformly distributing the test sample on the surface of the model, and placing the test sample on CO 2 Incubator (37 ℃, 5% CO) 2 ) And incubated for 24h. After the end of the dosing incubation, the model was fixed and frozen and the sections were fluorescent photographed.
Comparative example 5
The procedure of example 2 was followed, except that the molecular motor in the adenosine-containing anti-aging and penetration enhancing composition was replaced with an equivalent portion of azone. Collecting fibroblast in logarithmic growth phase according to 4×10 4 Cells/well were seeded into 24-well plates. In incubator (37 ℃, 5% CO) 2 ) Is cultured for 24 hours. Randomly divided into blank control groups,Negative control, adenosine + azone, each set was provided with 3 replicate wells. Culturing in incubator for 24 hr, and performing 30J/cm on the group with UVA irradiation 2 Is placed in an incubator for continuous cultivation for 24 hours. The ATP content of each cell group was measured by performing the procedure according to the instructions for the ATP kit.
Comparative example 6
The procedure of example 3 was followed, except that the molecular motor in the adenosine-containing anti-aging and penetration enhancing composition was replaced with an equivalent portion of azone. The fibroblasts in the logarithmic growth phase were collected and seeded at 4X 104 cells/well in 24-well plates. Culturing in an incubator (37 ℃, 5% co 2) for 24 hours. Randomly, a blank control group, a negative control group, an adenosine group, and adenosine +1, 2-pentanediol, each group was provided with 3 duplicate wells. After the dosing, the culture was continued for 24 hours in an incubator, and the UVA irradiation was performed for 30J/cm2 on the group with UVA irradiation, and the culture was continued for 24 hours in the incubator. The operation is carried out according to the instruction of the active oxygen kit, and the flow cytometry is used for machine detection.
Currently, the most widely used penetration enhancers for transdermal administration are propylene glycol, 1, 2-pentanediol, azone, and the like. 1, 2-pentanediol and azone are selected as permeation promoters of the comparative example to compare the permeation promoting effect of a molecular motor. The cumulative percutaneous permeation of adenosine by 1, 2-pentanediol, azone, and molecular motor is shown in Table 3, and the effects on the levels of ATP and ROS are shown in tables 4 and 5, respectively. The results show that the molecular motor has the best percutaneous permeation effect on the adenosine compared with the 1, 2-pentanediol and the azone, and the molecular motor synergistically increases the anti-wrinkle and anti-aging effects of the adenosine.
TABLE 3 Effect of different permeation enhancers on the transdermal effect of adenosine
| Sample name | Time point | Average fluorescence intensity |
| Adenosine | 24h | 113671.763±13100.008 |
| Adenosine + molecular motor | 24h | 314741.491±7329.200 |
| Adenosine +1,2 pentanediol | 24h | 175221.185±9802.759 |
| Adenosine + azone | 24h | 260104.035±10520.851 |
As can be seen from Table 3, 1, 2-pentanediol, azone and molecular motor all promote permeation of adenosine, but molecular motor has the best effect of promoting permeation of adenosine.
TABLE 4 Effect of different permeation enhancers on adenosine to promote ATP production
As can be seen from table 4, adenosine is effective in increasing ATP production in UVA-irradiated fibroblasts, the combination of 1, 2-pentanediol and adenosine, the combination of azone and adenosine, and the combination of molecular motor and adenosine promoted ATP production alone were not significantly different, but the combination of molecular motor and adenosine promoted ATP production significantly stronger than adenosine alone, indicating that the synergistic effect of molecular motor and adenosine on increasing ATP production in UVA-irradiated fibroblasts was present.
TABLE 5 Effect of different permeation enhancers on the inhibition of ROS formation by adenosine
| Adenosine | Adenosine + molecular motor | Adenosine +1,2 pentanediol | Adenosine + azone | |
| ROS inhibition rate | 35.75％ | 52.00％ | 33.56％ | 39.85％ |
As can be seen from table 5, the inhibition of ROS by the combination of 1, 2-pentanediol and adenosine, and the combination of azone and adenosine was 33.56% and 39.85%, respectively, compared to the inhibition of ROS by adenosine alone. The inhibition rate of the combination of the molecular motor and the adenosine on the generation of ROS by UVA stimulation is 52.00 percent, which is obviously higher than that of the adenosine alone, and the molecular motor and the adenosine have synergistic effect on the inhibition of the ROS generated by UVA.
Example 4
To better illustrate the applicability of the present invention, the adenosine-containing anti-aging and penetration-promoting composition prepared in example 1 of the present invention was added to an anti-aging cream at an addition level of 4%, and stability and safety tests were performed on the anti-aging cream.
The preparation process of the anti-aging eye cream comprises the following steps:
10g of glycerol stearate, 30g of cocoyl alcohol-caprylate/caprate, 60g of polydimethylsiloxane, 50g of behenyl alcohol and 20g of glycerol stearate citrate are put into an oil phase pot, heated to 80 ℃, stirred and dissolved for standby;
656g of water, 59g of 1, 3-propylene glycol, 30g of glycerol, 2.5g of dipotassium glycyrrhizinate, 4g of p-hydroxyacetophenone, 0.5g of EDTA disodium and 40g of the anti-aging and permeation-promoting composition are put into a water phase pot, heated to 80 ℃, stirred and dissolved for standby;
pumping the oil phase pot into the emulsifying pot, stirring for 20-25rpm/min, homogenizing for 2000-2500rpm/min, slowly pumping the water phase into the emulsifying pot, maintaining the homogenization for 5-10min after the pumping, and defoaming and cooling to 50 ℃;
adding 6g of carbomer sodium into the emulsifying pot, stirring at 20-25rpm/min, and homogenizing at 2000-2500rpm/min. Homogenizing for 3-5min. And (5) defoaming and cooling to 40 ℃.
Adding 2g of carnosine, 30g of conotoxin and essence into an emulsifying pot, stirring at 20-25rpm/min, homogenizing at 2000-2500rpm/min, defoaming, cooling to 35 ℃, and discharging.
The storage stability is generally evaluated according to GB/T29665-2013; preservative efficacy testing, using international standard ISO11930:2012 (cosmetic-microbiology-cosmetic product antimicrobial protection assessment) method; the safety test adopts corresponding technical means according to the requirements of cosmetic safety technical Specification (2015 edition) to detect the safety of heavy metals (lead, mercury, arsenic and cadmium) and risk substances (diethylene glycol and dioxane) and microorganisms in the anti-aging face cream.
The general storage stability test results are shown in Table 3, and the cream has no obvious change in appearance, smell and pH value in freeze thawing cycle and heat/cold resistance test, and has storage stability.
TABLE 3 results of anti-aging cream storage stability test
The results of the anti-aging cream preservative efficacy test are shown in Table 2 and show that the results pass the A standard in Table B.1 of ISO11930-2012 appendix B.
TABLE 4 anti-aging face cream preservative efficacy test results
The detection results of the safety of heavy metals (lead, mercury, arsenic and cadmium), risk substances (diethylene glycol and dioxane) and microorganisms in the anti-aging face cream are shown in tables 4 and 5. Lead, mercury, arsenic, cadmium, diethylene glycol and dioxane are not detected, and the microbial indexes meet the standards.
TABLE 4 detection results of anti-aging face cream heavy metals and Risk substances
TABLE 5 anti-aging face cream microbiological assay results
Finally, it is noted that the above embodiments are only for illustrating the technical solution of the present invention and not for limiting the same, and although the applicant has described the present invention in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications and equivalents may be made thereto without departing from the spirit and scope of the technical solution of the present invention, and it is intended to be covered by the scope of the claims of the present invention.
Example 5:
anti-aging effect detection
The effect of the use of the cream was evaluated by recruiting 10 volunteers (women aged 25 to 50 years without history of skin diseases and cosmetic allergies). After the volunteers were cleansing the face in the morning and evening, the anti-aging cream prepared as described above (example 4) was used on the face for a trial period of 5 days. All volunteers during this period did not change the original facial cleansing product and pattern except for the cream, and only basic moisturizing lotions were used. The anti-aging cream was produced by a professional evaluator with the company CK in Germany before, 1 day and 5 days after useMPA 580 noninvasive skin elasticity tester detects skin elasticity of the left cheek of volunteers. The smaller the parameter R0, the tighter the skin.
The anti-aging cream used by volunteers in Table 6 was prepared from the adenosine-containing anti-aging and penetration-promoting composition prepared in example 1 according to the method for preparing an anti-aging eye cream.
TABLE 6 test results
In the statistical analysis significance of table 5, the effect of 5 days of using the anti-aging cream on the skin elasticity of volunteers was judged using a one-factor repeated measurement anova method. As can be seen from table 5, the skin elasticity R0 value was significantly improved and the skin firmness was significantly improved after 1 day and 5 days of use of the test cream as compared to before use.
The preferred embodiments of the present invention have been described in detail above, but the present invention is not limited to the specific details of the above embodiments, and various simple modifications can be made to the technical solution of the present invention within the scope of the technical concept of the present invention, and all the simple modifications belong to the protection scope of the present invention.
In addition, the specific features described in the above embodiments may be combined in any suitable manner, and in order to avoid unnecessary repetition, various possible combinations are not described further.
The foregoing detailed description is directed to one of the possible embodiments of the present invention, which is not intended to limit the scope of the invention, but is to be accorded the full scope of all such equivalents and modifications so as not to depart from the scope of the invention.
Claims (9)
1. An adenosine-containing anti-aging and penetration-promoting composition, characterized in that: the composition comprises 1 to 15 weight parts of adenosine and 80 to 99 weight parts of molecular motor; the molecular motor refers to thermophilic thermus fermentation liquid.
2. An adenosine-containing anti-aging and penetration-promoting composition according to claim 1, wherein: the composition comprises 5-10 parts by weight of adenosine and 80-90 parts by weight of molecular motor.
3. An adenosine-containing anti-aging and penetration-promoting composition according to claim 1 or 2, wherein: the weight of the molecular motor is 5-15 times of that of the adenosine.
4. An adenosine-containing anti-aging and penetration-promoting composition according to claim 2, wherein: the composition comprises 1 part by weight of adenosine and 8 parts by weight of molecular motor.
5. Use of an adenosine-containing anti-aging and penetration-promoting composition for preparing an anti-aging cosmetic, characterized in that the adenosine-containing anti-aging and penetration-promoting composition according to any one of claims 1 to 4 is used.
6. The use of the adenosine-containing anti-aging and penetration-promoting composition according to claim 5 for preparing an anti-aging cosmetic, wherein the anti-aging and penetration-promoting composition is added into an anti-aging essence or cream-type cosmetic in an amount of 1 to 20 mass%.
7. An adenosine anti-aging and permeation-promoting facial cream is characterized by comprising 0.1-8 parts by weight of the adenosine-containing anti-aging and permeation-promoting composition according to claim 1, 1-5 parts by weight of conotoxin, 0.1-0.5 part by weight of carnosine, 0.1-0.5 part by weight of dipotassium glycyrrhizinate, 50-70 parts by weight of water and deionized water.
8. The method for preparing the adenosine anti-aging, permeation-promoting and anti-aging facial cream according to claim 7, which comprises the following steps:
s1: weighing the raw materials according to the component proportion of the anti-aging and permeation-promoting composition containing the adenosine, and uniformly mixing to obtain the anti-aging and permeation-promoting composition containing the adenosine;
s2: adding 0.1-10 parts by weight of glycerol stearate, 0.5-10 parts by weight of cocoyl alcohol-caprylate/caprate, 0.5-20 parts by weight of polydimethylsiloxane, 0.5-20 parts by weight of behenyl alcohol and 0.5-20 parts by weight of glycerol stearate citrate into an oil phase pot, heating to 60-90 ℃, stirring and dissolving for standby;
s3: adding 30-100 parts by weight of water, 0.5-20 parts by weight of 1, 3-propylene glycol, 0.5-20 parts by weight of glycerol, 0.05-3 parts by weight of dipotassium glycyrrhizinate, 0.1-20 parts by weight of p-hydroxyacetophenone, 0.01-5 parts by weight of EDTA disodium and 0.01-20 parts by weight of an adenosine-containing anti-aging and permeation-promoting composition into a water phase pot, heating to 60-90 ℃, stirring and dissolving for standby;
s4: pumping the oil phase pot into the emulsifying pot, stirring for 15-35rpm/min, opening homogenizing for 1500-3500rpm/min, pumping the water phase into the emulsifying pot, maintaining the homogenization for 3-20min after the pumping, defoaming, and cooling to 30-60deg.C;
s5: adding 0.05-3 parts by weight of carbomer sodium into an emulsifying pot, stirring for 10-45rpm/min, homogenizing for 1000-3500rpm/min, keeping the homogenization for 2-8min, and defoaming and cooling to 20-55 ℃;
s6: adding 0.01-3 parts by weight of carnosine and 0.05-15 parts by weight of conotoxin into an emulsifying pot, stirring at 10-45rpm/min, homogenizing at 1000-3500rpm/min, defoaming, cooling to 20-50deg.C, and making into adenosine permeation-promoting antiaging cream.
9. The method for preparing the adenosine anti-aging, permeation-promoting and anti-aging facial cream according to claim 7, which comprises the following steps:
s1: weighing the raw materials according to the component proportion of the anti-aging and permeation-promoting composition containing the adenosine, and uniformly mixing to obtain the anti-aging and permeation-promoting composition containing the adenosine;
s2: adding 0.5-5 parts by weight of glycerol stearate, 1-5 parts by weight of cocoyl-caprylate/caprate, 1-10 parts by weight of polydimethylsiloxane, 1-10 parts by weight of behenyl alcohol and 1-10 parts by weight of glycerol stearate citric acid ester into an oil phase pot, heating to 80 ℃, stirring and dissolving for standby;
s3: adding 50-70 parts by weight of water, 1-10 parts by weight of 1, 3-propylene glycol, 1-5 parts by weight of glycerol, 0.1-0.5 part by weight of dipotassium glycyrrhizinate, 0.4-1 part by weight of p-hydroxyacetophenone, 0.05-0.1 part by weight of EDTA disodium and 0.1-8 parts by weight of an adenosine-containing anti-aging and permeation-promoting composition into an aqueous phase pot, heating to 80 ℃, stirring and dissolving for standby;
s4: pumping the oil phase pot into the emulsifying pot, stirring for 20-25rpm/min, homogenizing for 2000-2500rpm/min, pumping the water phase into the emulsifying pot, homogenizing for 5-10min after pumping, defoaming, and cooling to 50deg.C;
s5: adding 0.1-1 weight part of carbomer sodium into an emulsifying pot, stirring at 20-25rpm/min, homogenizing at 2000-2500rpm/min, maintaining homogenizing for 3-5min, defoaming, and cooling to 40deg.C;
s6: adding 0.1-0.5 weight part of carnosine and 1-5 weight parts of conotoxin into an emulsifying pot, stirring at 20-25rpm/min, homogenizing at 2000-2500rpm/min, defoaming, cooling to 35 ℃, and obtaining the adenosine permeation-promoting anti-aging facial cream.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| US4923851A (en) * | 1987-12-29 | 1990-05-08 | Raymond A. Roncari | Composition of matter for increasing intracellular ATP levels and physical performance levels and for increasing the rate of wound repair |
| CN101856313A (en) * | 2008-12-30 | 2010-10-13 | 欧莱雅公司 | Association of monosaccharides and adenosine and its cosmetic use |
| CN112891241A (en) * | 2021-02-06 | 2021-06-04 | 武汉百思凯瑞生物科技有限公司 | Targeted mitochondrial skin anti-aging nano composition and preparation method and application thereof |
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| US20150025031A1 (en) * | 2012-03-05 | 2015-01-22 | Wake Forest University Health Sciences | Metabolic downregulation for cell survival |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4923851A (en) * | 1987-12-29 | 1990-05-08 | Raymond A. Roncari | Composition of matter for increasing intracellular ATP levels and physical performance levels and for increasing the rate of wound repair |
| CN101856313A (en) * | 2008-12-30 | 2010-10-13 | 欧莱雅公司 | Association of monosaccharides and adenosine and its cosmetic use |
| CN112891241A (en) * | 2021-02-06 | 2021-06-04 | 武汉百思凯瑞生物科技有限公司 | Targeted mitochondrial skin anti-aging nano composition and preparation method and application thereof |
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