CN111012711B

CN111012711B - Composition with skin repairing and anti-aging effects and skin care product thereof

Info

Publication number: CN111012711B
Application number: CN201911398840.7A
Authority: CN
Inventors: 刘忠; 杨涛
Original assignee: Guangzhou Yihe Biotechnology Co ltd; Jinan University
Current assignee: Guangzhou Yihe Biotechnology Co ltd; Jinan University
Priority date: 2019-12-30
Filing date: 2019-12-30
Publication date: 2022-07-12
Anticipated expiration: 2039-12-30
Also published as: CN111012711A

Abstract

The invention relates to the technical field of skin care products, and provides a composition with skin repair and anti-aging effects and a skin care product thereof, wherein the composition comprises scutellaria flavonoids and palmitoyl tripeptide-5, and based on an anti-aging mechanism related to mitochondrial function and a small molecular peptide multi-target repair mechanism, a scutellaria flavonoid extract with anti-aging activity and palmitoyl tripeptide-5 with repair activity are combined to help the skin to delay aging and accelerate self-repair of the skin to achieve the effects of repairing and anti-aging, and the active polypeptide palmitoyl tripeptide-5 can promote the permeability of scutellaria flavonoids to the skin and synergize.

Description

Composition with skin repairing and anti-aging effects and skin care product thereof

Technical Field

The invention relates to the technical field of skin care products, in particular to a composition with skin repairing and anti-aging effects and a skin care product thereof.

Background

Mitochondria are organelles that generate energy (ATP) by consuming oxygen, and although they have antioxidant defense systems, ROS produced continuously still damage mitochondrial DNA

(mtDNA). mtDNA mutates more frequently because it lacks histone protection and it is generally less efficient than nuclear DNA repair. Increased variation impairs mitochondrial function, thereby reducing energy production and inducing a vicious cycle, and defective respiratory chains produce more ROS, which in turn impairs the whole cell and accelerates skin aging. Therefore, mitochondria are not only the main site where cells perform oxidative phosphorylation and generate energy, but also are closely related to ROS generation, apoptosis, telomere, signal transduction and the like, are considered to play a key role in relieving oxidative stress and aging, and are important breakthrough for delaying aging of organisms.

Experiments show that the radix scutellariae flavone can reduce the levels of ROS and Lipid Peroxide (LPO) by enhancing a mitochondrial enzyme system and an antioxidant system, protect nuclear DNA and mtDNA from free radical damage, enhance the expression of a voltage-dependent anion channel and adenine nucleotide translocase protein, improve the dysfunction of a mitochondrial membrane permeability transition pore and play a stronger role in protecting mitochondria.

Many researches show that the small molecular polypeptide has the effects of repairing and resisting aging from a plurality of targets due to small molecular weight, high activity and easy absorption. Wherein, palmitoyl tripeptide-5, also called collagen peptide, is the cosmetic short peptide with the most extensive application. The palmitoyl tripeptide-5 serving as a signal peptide can promote the synthesis of collagen and the generation of extracellular matrix elastin by increasing the activity of stromal cells, improve the facial elasticity, remarkably reduce and lighten wrinkles, enhance the cell activity, improve the moisture content of skin, enhance the skin luster and generate the effects of repairing and resisting aging.

At present, no report on the combined use of the flavonoids of scutellaria and the active polypeptide palmitoyl tripeptide-5 for repairing and resisting aging exists.

Disclosure of Invention

Aiming at the defects in the prior art, the first purpose of the invention is to provide a composition with skin repairing and anti-aging effects, and the composition has the advantages of delaying skin aging and accelerating skin self-repair to repair and resist aging based on an anti-aging mechanism related to mitochondrial function and a small molecular peptide multi-target repair mechanism.

In order to achieve the purpose, the invention provides the following technical scheme:

a composition with skin repairing and antiaging effects comprises Scutellariae radix flavone and palmitoyl tripeptide-5.

Furthermore, the weight ratio of the radix scutellariae flavone to the palmitoyl tripeptide-5 is (1-5): 1.

Furthermore, the weight ratio of the radix scutellariae flavone to the palmitoyl tripeptide-5 is (1-3): 1.

Through a large amount of literature search and experiments, the inventor discovers that the combination of the scutellaria flavone extract with anti-aging activity and the palmitoyl tripeptide-5 with repairing activity fully exerts the effects of active substances and polypeptide, thereby promoting the generation of collagen, better helping skin to delay aging and accelerating skin self-repair to achieve the effects of repairing and resisting aging, and the composition has remarkable promoting effects on mitochondrial functions through mitochondrial protection and mitochondrial dependent antioxidant pathways, including the functions of clearing active oxygen, enhancing the activity of mitochondrial respiratory chain compound, maintaining mitochondrial membrane potential, promoting mitochondrial ATP synthesis, delaying the aging process of skin, accelerating skin self-repair and achieving the purposes of repairing and resisting aging. According to the research, the skin permeability of the scutellaria flavone in clinical application is poor, and the inventor unexpectedly finds that when the scutellaria flavone and the active polypeptide palmitoyl tripeptide-5 are used in combination, the active polypeptide palmitoyl tripeptide-5 can enhance the skin permeability of the scutellaria flavone and effectively improve the skin permeability, so that the composition added into a skin care product has better safety, better absorption and wider applicable population range.

Aiming at the defects in the prior art, the second purpose of the invention is to provide a skin care product with the effects of repairing and resisting aging, and the skin care product has the advantages of delaying skin aging and accelerating skin self-repair.

A skin care product with an anti-aging and repairing effect comprises the following preparation raw materials in parts by weight: the composition according to any one of claims 1 to 3, which comprises 0.01 to 5 parts by weight of the composition, 0.1 to 5 parts by weight of a humectant, 0.001 to 5 parts by weight of a pH adjuster, 5 to 10 parts by weight of an emulsifier, and 0.001 to 5 parts by weight of an antioxidant

0.001-5 parts of fat-soluble extract and 50-60 parts of water.

Further, the fat-soluble extract at least comprises one of a peony root extract, a loquat leaf extract and a marine microalgae extract.

Further, the moisturizer comprises at least one of aloe vera gel and sodium hyaluronate.

Further, the pH regulator comprises at least one of lactic acid and aminomethyl propanol.

Further, the pH value of the skin care product ranges from 3.0 to 8.0.

By adding 0.01-5 parts by weight of the composition into the skin care product, the composition can help skin delay aging, enhance skin elasticity and accelerate skin self-repair, and the scutellaria baicalensis flavone is high in bioavailability and high in product safety, so that the skin care product is wide in application range.

The skin care product has the advantages that the moisturizing agents such as aloe vera gel, sodium hyaluronate, allantoin and the like are added into the skin care product, so that the skin care product is good in moisturizing performance, the moisture of the skin is enhanced, and the emulsifying agents formed by sorbitan olivate, ethylhexyl palmitate, N-palmitoyl hydroxyproline cetyl ester, caprylic acid/capric acid triglyceride and the like are added into the skin care product, so that raw materials in the skin care product can be distributed uniformly, and the stability of the skin care product is enhanced.

The antioxidant such as ceramide 3 is added into the skin care product, so that the prepared skin care product is not easy to oxidize and deteriorate, and the effects of delaying skin aging and accelerating skin self-repair are improved.

Adding pH regulators such as lactic acid and aminomethyl propanol into the skin care product to regulate the pH value of the skin care product to 3.0-8.0, preferably 5.0-7.0, so that the prepared skin care product has good quality.

The skin care product is added with liposoluble extracts such as the peony root extract, the loquat leaf extract and the marine microalgae extract to promote the skin to absorb moisture and enhance the skin elasticity, and the mass ratio of the peony root extract to the loquat leaf extract to the marine microalgae extract is 1:1 (1-2), so that the skin repairing effect is better, and the skin elasticity enhancement effect is better.

Further, the skin care product is repair anti-aging essence, essence milk, freeze-dried powder, facial mask or cream.

Further, the preparation method of the skin care product comprises the following steps:

1) putting the emulsifier and the fat-soluble extract into a dissolving pot, heating to 70-80 ℃, uniformly dissolving, and then preserving heat to prepare a phase A;

2) adding a humectant and an antioxidant into water, heating to 90-100 ℃, fully dissolving, and then cooling to 70-80 ℃ to prepare a phase B;

3) slowly adding the phase A prepared in the step 1) into the phase B prepared in the step 2), maintaining the temperature at 70-80 ℃, stirring for 10-15 min, controlling the stirring speed at 30-60 rpm, then homogenizing for 5-10 min at high speed, keeping the temperature, stirring for 15-30 min, and controlling the stirring speed at 60-80 rpm;

4) cooling to 40-50 ℃, adding the composition and the pH regulator, and uniformly stirring to obtain the skin care product with the anti-aging and repairing effects.

Compared with the prior art, the invention has the following beneficial effects:

1. the composition with the effects of repairing and resisting aging provided by the invention is prepared by combining the scutellaria flavone extract with the anti-aging activity and the palmitoyl tripeptide-5 with the repairing activity, so that the composition can better help the skin to delay aging and accelerate the self-repair of the skin to achieve the effects of repairing and resisting aging.

2. When the baicalin and the active polypeptide palmitoyl tripeptide-5 are used together, the active polypeptide palmitoyl tripeptide-5 can enhance the permeability of the baicalin to the skin and effectively improve the permeability of the skin, so that the composition is added into a skin care product and has better safety.

3. The repairing and anti-aging essence, essence milk, freeze-dried powder, mask or cream prepared by compounding the composition provided by the invention has the effects of resisting aging and repairing skin, and can enhance the skin elasticity.

Drawings

FIG. 1 is a graph of the results of the ROS scavenging experiment for the composition of example 3;

FIG. 2 is a graph showing the effect of the composition of example 3 on the activity of mitochondrial respiratory chain complexes I-IV;

FIG. 3 is a graph showing the results of the mitochondrial membrane potential measurement of the composition of example 3;

FIG. 4 is a graph showing the results of the measurement of mitochondrial ATP levels with the composition of example 3.

Detailed Description

The present invention will be described in further detail with reference to the drawings and examples.

Extracting the baicalein:

(1) pretreatment of scutellaria baicalensis

The radix scutellariae (inner Mongolian radix scutellariae) is washed twice by tap water and then washed twice by distilled feed water. Oven drying at 40 deg.C in oven, air drying the dried Scutellariae radix, pulverizing the air dried Scutellariae radix decoction pieces with Chinese medicinal pulverizer, and sieving with 10-20 mesh sieve. Obtaining Scutellariae radix granule with particle size of 10-20 mesh, sealing Scutellariae radix granule with valve bag, and placing in drying dish for use.

(2) Alcohol extraction method

Putting 5.0g of the pretreated scutellaria baicalensis medicinal material into a 250mL round-bottom flask, adding 100mL of 60% ethanol aqueous solution as an extraction solvent, putting the flask into a digital display constant-temperature water bath, performing reflux extraction at 80 ℃ for 1h, performing suction filtration after extraction, adding 60% ethanol aqueous solution into filter residues, performing re-extraction again, wherein the extraction conditions are the same as the first extraction conditions, and repeating the extraction for 5 times to obtain the scutellaria baicalensis flavone.

Palmitoyl tripeptide-5 of the formula C₃₃H₆₅N₅O₅White powder, molecular weight 611.9, purchased from Nanjing Sibaike Biochemical industries, Inc.

Note: the sum of the mass percentage of the skin care products is 100 percent.

Examples 1 to 4 of the composition having skin-repairing and anti-aging effects of the invention

The formulation of the composition of the present invention is shown in table 1 below.

TABLE 1

Example 5-8 repair anti-aging essence

The formula of the repair and anti-aging essence in the embodiments 5-8 of the invention is shown in the table 2.

TABLE 2

Note: "to" means not added to the repair and anti-aging essence.

The preparation method of the repair and anti-aging essence comprises the following steps:

1) adding the sodium hyaluronate component into water, stirring uniformly, heating to 80-85 ℃, preserving heat and stirring to disperse uniformly, and fully dissolving;

2) reducing the temperature to 40 ℃, then adding the glycerol, the ceramide 3, the caprylyl glycol and the composition into the step 1), maintaining the temperature at 40 ℃, stirring for 15min at the stirring speed of 60rpm, and adjusting the pH value of the solution to 5.5 by using aminomethyl propanol to obtain the repair and anti-aging essence.

Example 9-12 repair anti-aging essence milk

The formula of the repair and anti-aging essence milk in the embodiments 9-12 of the invention is shown in table 3.

TABLE 3

Note: "to" means not added to the repair and anti-aging essence milk.

The preparation method of the repair and anti-aging essence milk comprises the following steps:

1) adding sorbitan olivate, N-palmitoyl hydroxyproline cetyl ester, ethylhexyl palmitate, caprylic/capric triglyceride, allantoin and peony root extract into a dissolving pot, heating to 80 deg.C, dissolving uniformly, and keeping temperature to obtain phase A;

2) adding butanediol, phenoxyethanol, hydrolyzed sodium hyaluronate and other components into deionized water, heating to 90 deg.C, dissolving completely, and cooling to 80 deg.C to obtain phase B;

3) slowly adding the phase A prepared in the step 1) into the phase B prepared in the step 2), maintaining the temperature at 70-80 ℃, stirring for 15min, controlling the stirring speed at 60rpm, then homogenizing at high speed for 10min, preserving heat and stirring for 30min, and controlling the stirring speed at 80 rpm;

4) cooling to 40 ℃, adding the phase C and the phase D, adjusting the pH value of the solution to 5.6, uniformly stirring, discharging to obtain the repair anti-aging essence milk, and subpackaging into 100 vials according to the volume of 15mL per vial.

Comparative example 1

In accordance with example 11, comparative example 1 differs from example 11 only in that the restorative anti-aging cream does not contain a composition.

Comparative example 2

In accordance with example 11, comparative example 2 differs from example 11 only in that the repaired anti-aging cream does not contain palmitoyl tripeptide-5.

Comparative example 3

In accordance with example 11, comparative example 3 is different from example 11 only in that the rejuvenating and anti-aging serum does not contain scutellaria flavonoids.

Test example I, in vitro percutaneous absorption test

1. Test materials:

group A: 2mL of radix scutellariae flavone;

group B: palmitoyl tripeptide-52 mL;

group C: 2mL of radix scutellariae flavone and 52 mL of palmitoyl tripeptide;

group D: 6mL of radix scutellariae flavone and 52 mL of palmitoyl tripeptide;

group E: 10mL of radix scutellariae flavone and 52 mL of palmitoyl tripeptide.

2. The test method comprises the following steps:

1) preparation of ex vivo mouse skin: removing the chest and abdomen hair of ICR mouse (25 + -5 g), cutting off the chest and abdomen skin, removing subcutaneous fat and mucus tissue, cleaning, placing in normal saline, and storing in refrigerator at 4 deg.C. Before the experiment, the mixture is naturally thawed and soaked in normal saline.

2) In vitro transdermal absorption test method: mouse skin is fixed in Franz diffusion chamber (diffusion area 2.1 cm)²13.5ml) and a receiving tank, with the horny layer facing upwards, adding the above substances into the supplying tank, adding 30% ethanol physiological saline as receiving medium into the receiving tank, discharging air bubbles to make the liquid level just contact with the dermis layer of skin, adjusting the stirring speed of Franz diffusion chamber to 300r/min, the water bath temperature to (32 +/-1) DEG C, and discharging ethanol physiological saline from the receiving tank within 24hTaking out all the aqueous solution, evaporating to dryness in water bath, diluting to 5ml with methanol, and supplementing equal amount of receiving solution to meet the condition of leakage. And determining the content of the scutellaria flavone and palmitoyl tripeptide-5 in the sample by adopting a high performance liquid chromatography.

The radix Scutellariae flavone permeability (amount of radix Scutellariae flavone in the receiving pool/amount of radix Scutellariae flavone added) is 100%

Palmitoyl tripeptide-5 transmittance (amount of palmitoyl tripeptide-5 in the receiving pool/amount of palmitoyl tripeptide-5 added) × 100%

3. And (3) test results: as shown in table 5.

TABLE 5 in vitro transdermal absorption test results

As is clear from the test data in table 5, the group D of the present invention contains baicalein and the active polypeptide palmitoyl tripeptide-5, and the weight ratio of baicalein to palmitoyl tripeptide-5 is 2:1, which is the most permeable to skin, and the permeation amount of baicalein is significantly increased compared to the group containing no palmitoyl tripeptide-5, indicating that the combination of the two components exerts a synergistic effect to promote the permeation of baicalein into skin.

Test example II ROS scavenging test of the composition having skin-repairing and anti-aging effects of the present invention

1. Test materials:

example 3 composition with skin repair anti-aging effect.

2. The test method comprises the following steps:

the massive production of ROS is the early reaction of apoptosis, mitochondria are the main production site of ROS, more than 90% of oxygen molecules in organisms are consumed in mitochondria, and the probe H is utilized in the test example₂DCFDA detects ROS scavenging ability of Scutellariae radix flavone. H₂DCFDA excites green light, the brighter the green light, the higher the active oxygen content. Cells were cultured in 96-well plates, pretreated for 16h by adding different concentrations of composition solutions (1, 2, 4, 6, 8, 10. mu.g/ml) followed by 400. mu. M H₂O₂And (5) incubating for 24 h. Addition of H₂DCFDA (final concentration 50. mu.M) is cultured for another 30min and excited by 488nM in a multifunctional microplate reader, and fluorescence intensity of each well of the culture plate is detected by 525nM emission wavelength. Each set is provided with 6 multiple holes, refer to fig. 1.

As can be seen from FIG. 1, the relative amount of ROS treated with the composition is significantly reduced compared to the model group, and the relative amount of ROS treated with the sample at a concentration of 10 μ g/ml is about 50% of that of the model group, which is statistically significant (p <0.01), indicating that the composition of example 3 has a significant ROS scavenging effect. In addition, the relative content of ROS is reduced along with the increase of the concentration of the sample, and a remarkable dose-effect relationship is shown. The result shows that the composition consisting of the flavonoids of the scutellaria and the active polypeptide palmitoyl tripeptide-5 has antioxidant activity and can eliminate redundant free radicals in cells.

Test example III determination of the Activity of the compositions having skin-repairing anti-aging Effect on mitochondrial respiratory chain complexes I to IV

1. Test materials:

example 3 composition with skin repair anti-aging effect.

2. The test method comprises the following steps: cells were cultured in 24-well plates and after 24h, different concentrations of composition solutions (1, 5, 10. mu.g/ml) were added and incubation continued for 48 h. Adding mitochondria extraction medium, extracting cell mitochondria by adopting a repeated freeze-thaw method, measuring protein content by using a BCA method, and adjusting each group of mitochondria extracting solution to ensure that the protein content is basically consistent. Mitochondrial Respiratory Chain Complex (MRCC) I-IV activity was examined with a kit, see FIG. 2.

Mitochondria are the main site for aerobic respiration and provide energy for cell life activities. The respiratory chain complex is the site for the transfer of electrons and the execution of mitochondrial functions, and its activity has an important influence on mitochondrial function and cell status. As shown in figure 2, the 10 mu g/ml composition solution can obviously improve the activity of MRCC I, III and IV (p is less than 0.01), and has no obvious influence on MRCC II, and the result shows that the composition consisting of the radix scutellariae flavone and the active polypeptide palmitoyl tripeptide-5 has a certain promotion effect on the function of the mitochondrial respiratory chain.

Test example four measurement of mitochondrial Membrane potential with the composition having skin repairing and anti-aging effects of the present invention

1. Test materials:

example 3 composition with skin repair anti-aging effect.

2. The test method comprises the following steps: the transmembrane potential of mitochondria is an important indicator of the state of mitochondrial function, and its decline is considered to be the earliest event in the course of the apoptosis cascade. Rhodamine 123(Rh123) is a dye capable of staining mitochondria of living cells, has fluorescent cations, can penetrate cell membranes, enters a mitochondrial matrix by depending on the transmembrane potential of the mitochondria, and is neutralized with H + to quench fluorescence. When the trans-membrane potential of mitochondria is reduced or lost, Rh123 can be released from mitochondria again to emit yellow green fluorescence. Cells were cultured in 24-well plates, and after 16h, the cells were pretreated by adding composition solutions (1, 2, 4, 6, 8, 10. mu.g/ml) at different concentrations for 1h, and then incubated for 24h by adding 400. mu. M H2O2, and the change of mitochondrial membrane potential was detected by using the fluorescent dye Rh123, as shown in FIG. 3.

The larger the fluorescence value, the lower the mitochondrial membrane potential, i.e., the lower the mitochondrial activity. The upper graph shows that the fluorescence of the cells is obviously enhanced and the mitochondrial membrane potential is reduced after the H2O2 treatment compared with the normal cells, and the fluorescence value of the solution of the composition of example 3 of 6, 8 and 10 mu g/ml is lower than that of the model group after the pretreatment, and the difference has statistical significance (p is less than 0.01), which indicates that the mitochondrial membrane potential is increased, and the composition consisting of the baical skullcap flavone and the active polypeptide palmitoyl tripeptide-5 can obviously maintain the mitochondrial function activity.

Test example five, the detection of mitochondrial ATP content by the composition with skin repairing and anti-aging effects of the invention

1. Test materials:

example 3 composition with skin repair anti-aging effect.

2. The test method comprises the following steps: intracellular ATP levels are an important indicator of cellular energy metabolism, and ATP levels are markedly reduced in senescent cells. The determination of cellular ATP content by luciferase (luciferase) -luciferin (luciferin) system is one of the most sensitive and specific methods in the current bioenergy research, and the principles are luciferase (E), reduced luciferin (LH2) and luciferase (LH2)Mg²⁺And reacting under the participation of oxygen, wherein the generated light quantum h is in positive correlation with a certain amount of ATP. Cells were cultured in 24-well plates, pretreated for 16h by adding different concentrations of composition solutions (1, 2, 4, 6, 8, 10. mu.g/ml) followed by 400. mu. M H₂O₂And (5) incubating for 24 h. The intracellular ATP content was measured using an ATP detection kit, and FIG. 4 shows the results.

The experimental results show that H is compared with normal cells₂O₂After treatment, the ATP in the cells is obviously reduced, and after the cells are pretreated by 6, 8 and 10 mu g/ml composition solution, the ATP concentration in the cells is increased in a gradient manner. The experimental results show that the composition of flavonoids of scutellaria and active polypeptide palmitoyl tripeptide-5 can maintain normal ATP synthesis under the condition of cell oxidative damage.

Test example six, use test-human skin elasticity test

1. Test materials:

the repairing and anti-aging essence obtained in experimental examples 5-8, the repairing and anti-aging essence emulsions obtained in experimental examples 9-12, and the repairing and anti-aging essence emulsions obtained in comparative examples 1-3.

2. The test method comprises the following steps:

the samples obtained in experimental examples 2 to 9 and comparative examples 1 to 3 were applied to the skin of 20 women (30 to 55 years old) 2 times a day (morning and evening) for 2 months without applying any other product, and the blank control group was not applied with any sample. The German CK skin tester is used for detecting the skin elasticity after smearing samples for 7, 15, 30 and 60 days, recording statistics and carrying out significant difference analysis, and the judging method is as follows: no significant difference was assigned a score of 0, significant difference was assigned a score of 1 with p <0.1, significant difference was assigned a score of 2 with p <0.5, significant difference was assigned a score of 3 with p < 0.01. The test results are as follows:

TABLE 4 score of skin elasticity improvement effect of each of the experimental examples and comparative examples

The test data in table 4 show that each of the experimental examples 5 to 12 can significantly enhance the skin elasticity, and the improvement effect is more significant with the extension of the use time, wherein the repair anti-aging essence has the best repair effect in example 7, and the repair anti-aging essence has the best anti-aging repair effect in example 11.

The comparative example 1 does not contain the composition, which shows that the composition can delay the aging process of skin, accelerate the self-repair of skin and enhance the elasticity of skin, and if the composition only contains the scutellaria flavone extract or only contains the active polypeptide palmitoyl tripeptide-5, the anti-aging and skin-repairing effects are poor and the skin elasticity is poor.

The embodiments of the present invention are preferred embodiments of the present invention, and the scope of the present invention is not limited by these embodiments, so: all equivalent changes made according to the structure, shape and principle of the invention are covered by the protection scope of the invention.

Claims

1. A composition with skin repairing and antiaging effects comprises Scutellariae radix flavone extract and palmitoyl tripeptide-5; the weight ratio of the scutellaria baicalensis flavone extract to the palmitoyl tripeptide-5 is 3: 1;

the extraction method of the scutellaria baicalensis flavone extract comprises the following steps:

(1) pretreatment of scutellaria baicalensis

Washing radix Scutellariae with tap water for 2 times, washing with distilled water for 2 times, oven drying at 40 deg.C, air drying, pulverizing with a Chinese medicinal pulverizer, sieving with 10-20 mesh sieve to obtain Scutellariae radix granules with particle size of 10-20 meshes, sealing Scutellariae radix granules with self-sealing bag, and placing in a drying vessel;

(2) alcohol extraction method

Putting 5.0g of the pretreated scutellaria baicalensis medicinal material into a 250mL round-bottom flask, adding 100mL of 60% ethanol aqueous solution serving as an extraction solvent, putting the round-bottom flask into a digital display constant-temperature water bath, performing reflux extraction at 80 ℃ for 1h, and performing suction filtration after extraction; extracting the residue with 60% ethanol water solution under the same conditions as the first extraction for 5 times to obtain Scutellariae radix flavone extract.

2. The skin care product with the anti-aging and repairing effects is characterized by comprising the following preparation raw materials in parts by weight: the composition according to claim 1, which comprises 0.01 to 5 parts of a humectant, 0.1 to 5 parts of a pH adjuster, 5 to 10 parts of an emulsifier, 0.001 to 5 parts of an antioxidant, 0.001 to 5 parts of a fat-soluble extract, and 50 to 60 parts of water.

3. The skin care product of claim 2, wherein the lipid soluble extract comprises at least one of a peony root extract, a loquat leaf extract, and a marine microalgae extract.

4. The skin care product of claim 2, wherein the moisturizer comprises at least one of aloe vera gel and sodium hyaluronate.

5. The skin care product of claim 2, wherein the pH adjusting agent comprises at least one of lactic acid and aminomethyl propanol.

6. The skin care product of claim 2, wherein the pH of the skin care product is in the range of 3.0 to 8.0.

7. The skin care product according to any one of claims 2 to 6, wherein the skin care product is a repair anti-aging essence, an essence emulsion, a freeze-dried powder, a mask or a cream.

8. A skin care product according to any one of claims 2 to 6, characterized in that the preparation method comprises the steps of: