CN115380857A - Prawn larva incubation method - Google Patents
Prawn larva incubation method Download PDFInfo
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- CN115380857A CN115380857A CN202211037503.7A CN202211037503A CN115380857A CN 115380857 A CN115380857 A CN 115380857A CN 202211037503 A CN202211037503 A CN 202211037503A CN 115380857 A CN115380857 A CN 115380857A
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- 241000238557 Decapoda Species 0.000 title claims abstract description 77
- 238000011534 incubation Methods 0.000 title claims abstract description 22
- 230000012447 hatching Effects 0.000 claims abstract description 96
- 235000013601 eggs Nutrition 0.000 claims abstract description 43
- 238000000034 method Methods 0.000 claims abstract description 26
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 39
- 239000003795 chemical substances by application Substances 0.000 claims description 35
- 238000005728 strengthening Methods 0.000 claims description 24
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 claims description 18
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 18
- 239000001301 oxygen Substances 0.000 claims description 18
- 229910052760 oxygen Inorganic materials 0.000 claims description 18
- CPKVUHPKYQGHMW-UHFFFAOYSA-N 1-ethenylpyrrolidin-2-one;molecular iodine Chemical compound II.C=CN1CCCC1=O CPKVUHPKYQGHMW-UHFFFAOYSA-N 0.000 claims description 16
- 229920000153 Povidone-iodine Polymers 0.000 claims description 16
- 239000012286 potassium permanganate Substances 0.000 claims description 16
- 229960001621 povidone-iodine Drugs 0.000 claims description 16
- 239000004342 Benzoyl peroxide Substances 0.000 claims description 14
- OMPJBNCRMGITSC-UHFFFAOYSA-N Benzoylperoxide Chemical compound C=1C=CC=CC=1C(=O)OOC(=O)C1=CC=CC=C1 OMPJBNCRMGITSC-UHFFFAOYSA-N 0.000 claims description 14
- 235000019400 benzoyl peroxide Nutrition 0.000 claims description 14
- XUIIKFGFIJCVMT-GFCCVEGCSA-N D-thyroxine Chemical group IC1=CC(C[C@@H](N)C(O)=O)=CC(I)=C1OC1=CC(I)=C(O)C(I)=C1 XUIIKFGFIJCVMT-GFCCVEGCSA-N 0.000 claims description 12
- 238000002791 soaking Methods 0.000 claims description 12
- 229940034208 thyroxine Drugs 0.000 claims description 12
- XUIIKFGFIJCVMT-UHFFFAOYSA-N thyroxine-binding globulin Natural products IC1=CC(CC([NH3+])C([O-])=O)=CC(I)=C1OC1=CC(I)=C(O)C(I)=C1 XUIIKFGFIJCVMT-UHFFFAOYSA-N 0.000 claims description 12
- 235000010469 Glycine max Nutrition 0.000 claims description 11
- 244000068988 Glycine max Species 0.000 claims description 11
- GOMNOOKGLZYEJT-UHFFFAOYSA-N isoflavone Chemical compound C=1OC2=CC=CC=C2C(=O)C=1C1=CC=CC=C1 GOMNOOKGLZYEJT-UHFFFAOYSA-N 0.000 claims description 11
- CJWQYWQDLBZGPD-UHFFFAOYSA-N isoflavone Natural products C1=C(OC)C(OC)=CC(OC)=C1C1=COC2=C(C=CC(C)(C)O3)C3=C(OC)C=C2C1=O CJWQYWQDLBZGPD-UHFFFAOYSA-N 0.000 claims description 11
- 235000008696 isoflavones Nutrition 0.000 claims description 11
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims description 9
- 238000005286 illumination Methods 0.000 claims description 9
- 235000017281 sodium acetate Nutrition 0.000 claims description 9
- 239000001632 sodium acetate Substances 0.000 claims description 9
- JVMRPSJZNHXORP-UHFFFAOYSA-N ON=O.ON=O.ON=O.N Chemical compound ON=O.ON=O.ON=O.N JVMRPSJZNHXORP-UHFFFAOYSA-N 0.000 claims description 8
- UCTWMZQNUQWSLP-UHFFFAOYSA-N adrenaline Chemical compound CNCC(O)C1=CC=C(O)C(O)=C1 UCTWMZQNUQWSLP-UHFFFAOYSA-N 0.000 claims description 8
- 238000011161 development Methods 0.000 claims description 3
- 230000018109 developmental process Effects 0.000 claims description 3
- 238000007598 dipping method Methods 0.000 claims description 3
- 210000002257 embryonic structure Anatomy 0.000 claims 1
- 238000003756 stirring Methods 0.000 claims 1
- 208000031320 Teratogenesis Diseases 0.000 abstract description 3
- 238000012258 culturing Methods 0.000 description 14
- 230000000052 comparative effect Effects 0.000 description 10
- UCTWMZQNUQWSLP-VIFPVBQESA-N (R)-adrenaline Chemical compound CNC[C@H](O)C1=CC=C(O)C(O)=C1 UCTWMZQNUQWSLP-VIFPVBQESA-N 0.000 description 8
- 229930182837 (R)-adrenaline Natural products 0.000 description 8
- 229960005139 epinephrine Drugs 0.000 description 8
- 238000003287 bathing Methods 0.000 description 5
- 238000007654 immersion Methods 0.000 description 5
- 239000012528 membrane Substances 0.000 description 4
- 230000004075 alteration Effects 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000033001 locomotion Effects 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 244000052769 pathogen Species 0.000 description 2
- 230000005570 vertical transmission Effects 0.000 description 2
- 241000238421 Arthropoda Species 0.000 description 1
- 241000995704 Fenneropenaeus chinensis Species 0.000 description 1
- 241000219470 Mirabilis Species 0.000 description 1
- 241000832310 Palaemon macrodactylus Species 0.000 description 1
- 241000238550 Penaeidae Species 0.000 description 1
- 241000927735 Penaeus Species 0.000 description 1
- 241000238552 Penaeus monodon Species 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 210000002969 egg yolk Anatomy 0.000 description 1
- 230000013020 embryo development Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000009191 jumping Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 210000004798 organs belonging to the digestive system Anatomy 0.000 description 1
- 230000029264 phototaxis Effects 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/50—Culture of aquatic animals of shellfish
- A01K61/59—Culture of aquatic animals of shellfish of crustaceans, e.g. lobsters or shrimps
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
- A01K61/13—Prevention or treatment of fish diseases
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
- A01K61/17—Hatching, e.g. incubators
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention provides a prawn larva incubation method, which comprises the following steps: the hatching method can improve the hatching rate of the prawns and reduce the teratogenesis rate of fertilized eggs, the hatching rate of the fertilized eggs of the prawns reaches 98.2 percent, the teratogenesis rate of the larvae is only 1.3 percent, and the healthy and qualified prawns can be cultured.
Description
Technical Field
The invention relates to the technical field of aquaculture, in particular to a prawn larva incubation method.
Background
The prawns, the academic name of the oriental shrimps, also known as Chinese shrimps, penaeus chinensis and Penaeus monodon, are shrimps of the genus Penaeus of the family Penaeidae, the phylum Arthropoda, class Mirabilis, order Nopoda. Prawns are large in size, commonly called prawn; according to the production and development process of prawn, the prawn larva undergoes the processes of sperm egg-cell stage-blastocyst stage-gastral stage-branch bud stage-intramembranous nauplii stage-nauplii-flea larva-mysorethorn larva stage-young prawn, wherein the nauplii are small in body, are not segmented, have 3 pairs of short appendages, do 'jumping' movement in water, have paired tail spines at the tail ends, do not have complete oral organs and digestive organs, rely on the yolk of the larvae to maintain the vital movement, and have strong phototaxis. In recent years, the prawn culture industry is vigorously developed in China, wherein the incubation of prawn larvae is an important link for prawn culture, and high-quality nauplii are the premise of improving the survival rate of seedling culture and the key point for incubating healthy prawn larvae, so that the most reassuring prawn larvae can be provided for farmers, and the maximum economic value is created. At present, the single yield of each healthy and high-quality parent shrimp can reach 25-35 million fertilized eggs, and under the artificial environment, the fertilized eggs have low hatching rate and high larva aberration rate due to a plurality of factors, and the phenomena of asynchronous larva distortion, weak vitality and the like occur in a seedling raising link.
Disclosure of Invention
In view of this, the invention provides a prawn larva incubation method, which solves the above problems.
The technical scheme of the invention is realized as follows: a prawn larva incubation method comprises the following steps: the method comprises the following steps:
s1, setting an environment: arranging an incubation pool, adding 5-8 mg/L sodium acetate into the incubation pool after the water depth is 0.7-1.2 m, and airing the incubation pool for 2-3 days for later use;
s2, hatching fertilized eggs: soaking the fertilized eggs in a strengthening solution, wherein the strengthening solution is povidone iodine, potassium permanganate and benzoyl peroxide in a volume ratio of 3-5 to 1-5 2 Ten thousand per liter, the eggs are transferred into the hatching pond after immersion bath, the water temperature of the hatching pond is set to be 28-35 ℃, the water salinity is set to be 20-30 per thousand, the dissolved oxygen is 5-7 mg/L, the illumination intensity is 20-40 Lx, and the ammonia nitrogen is less than 0.5mg/L, a hatching agent is added for hatching, the pond water is stirred by an egg stirrer every 1-2 hours, and the embryo development condition is checked at regular time until fertilized eggs develop into larvae;
s3, larva cultivation: feeding the larva into a seedling raising pond, wherein the cultivation density is (10-14) multiplied by 10 4 Tail/m 3 Culturing at 28-32 deg.c and pH 7.8-8.4, dissolved oxygen over 5mg/L and ammonia nitrogen below 0.1mg/L, and nitriteUnder 0.05mg/L of nitrogen and with 26-35 per mill of salinity, the shrimps are moved out of the culture pond after being cultured into young shrimps and then are cultured into grown shrimps.
Furthermore, the mass concentration of the povidone iodine is 5-10 mg/L, the mass concentration of the potassium permanganate is 10-20 mg/L, and the mass concentration of the benzoyl peroxide is 3-8 mg/L.
Further, the strengthening solution is povidone iodine, potassium permanganate and benzoyl peroxide in a volume ratio of 4.
Further, the hatching agent is thyroxine, adrenaline and soybean isoflavone with the volume ratio of 15-20.
Further, the hatching agent is thyroxine, epinephrine and soybean isoflavone in a volume ratio of 18.
Further, the addition amount of the hatching agent is 5-8% of the weight of the shrimps.
Compared with the prior art, the invention has the beneficial effects that:
the hatching method can improve the hatching rate of the prawns and reduce the deformity rate of the fertilized eggs, the prepared strengthening solution is bathed in a certain proportion, various diseases of the prawns are prevented to a great extent by combining the environmental conditions of the bathing, the vertical transmission of pathogens is prevented, and the hatching method has an obvious effect of removing bacteria attached to the fertilized eggs; meanwhile, the egg membrane is rapidly oxidized and gradually cornified, the texture of the egg membrane becomes relatively hard, and the egg membrane is prevented from being damaged in the hatching process; the hatching agent is combined with a hatching method, so that the hatching rate of fertilized eggs of the prawns can be effectively improved to 98.2 percent, the larva aberration rate is only 1.3 percent, and the healthy and qualified prawns can be cultured.
Detailed Description
In order to better understand the technical content of the invention, specific examples are provided below to further illustrate the invention.
The experimental methods used in the examples of the present invention are all conventional methods unless otherwise specified.
The materials, reagents and the like used in the examples of the present invention are commercially available unless otherwise specified.
Example 1
A prawn larva incubation method comprises the following steps: the method comprises the following steps:
s1, setting an environment: arranging a hatching pond, adding sodium acetate of 5mg/L into the hatching pond with the water depth of 1m, and airing for 2d for later use;
s2, hatching fertilized eggs: soaking the fertilized eggs in a strengthening solution, wherein the strengthening solution is povidone iodine, potassium permanganate and benzoyl peroxide in a volume ratio of 3 2 Ten thousand per m 3 Transferring the shrimp to the hatching pond after immersion bath, setting the water temperature of the hatching pond to be 28 ℃, the water quality and the salinity to be 20 thousandths, the dissolved oxygen to be 5mg/L, the illumination intensity to be 200Lx and the ammonia nitrogen to be less than 0.5mg/L, putting a hatching agent into the hatching pond for hatching, wherein the adding amount of the hatching agent is 5 percent of the weight of the shrimp, the hatching agent is thyroxine, epinephrine and soybean isoflavone with the volume ratio of 15;
s3, larva cultivation: feeding the larva into a nursery pond with a cultivation density of 10 × 10 4 Tail/m 3 Culturing water at 28 ℃, pH 7.8, dissolved oxygen above 5mg/L, ammonia nitrogen below 0.1mg/L, nitrite nitrogen below 0.05mg/L and salinity below 26 per mill, and removing the shrimp into a culture pond for culturing the shrimp into adult shrimps.
Example 2
A prawn larva incubation method comprises the following steps: the method comprises the following steps:
s1, setting an environment: arranging a hatching pond, adding 8mg/L of sodium acetate into the hatching pond with the water depth of 1m, and airing for 3d for later use;
s2, hatching fertilized eggs: and (2) dipping the fertilized eggs in a strengthening solution, wherein the strengthening solution is povidone iodine, potassium permanganate and benzoyl peroxide in a volume ratio of 5 2 Ten thousand per m 3 Transferring into the above hatching pond after bathing, setting water temperature of the hatching pond at 35 deg.C, water salinity of 30 ‰, and dissolved oxygen content of 7mg/L, illumination intensity of 40Lx and ammonia nitrogen of less than 0.5mg/L, adding an incubation agent for incubation, wherein the addition amount of the incubation agent is 8 percent of the weight of the shrimps, the incubation agent is thyroxine, adrenaline and soybean isoflavone, the volume ratio of the incubation agent to the incubation agent is 20;
s3, larva cultivation: feeding the larva into a nursery pond with a cultivation density of 14 × 10 4 Tail/m 3 The temperature of the cultivation water is 32 ℃, the pH value is 8.4, the dissolved oxygen is more than 5mg/L, the ammonia nitrogen is less than 0.1mg/L, the nitrite nitrogen is less than 0.05mg/L, and the salinity is 35 per mill.
Example 3
A prawn larva incubation method comprises the following steps: the method comprises the following steps:
s1, setting an environment: arranging a hatching pond, adding 7mg/L of sodium acetate into the hatching pond with the water depth of 1m, and airing for 3d for later use;
s2, hatching fertilized eggs: and (2) soaking the fertilized eggs in a strengthening solution, wherein the strengthening solution is povidone iodine, potassium permanganate and benzoyl peroxide in a volume ratio of 4 2 Ten thousand per m 3 Transferring the shrimp into the hatching pond after immersion bath, setting the water temperature of the hatching pond at 30 ℃, the salinity of water at 25 per mill, the dissolved oxygen at 6mg/L, the illumination intensity at 30Lx and the ammonia nitrogen at less than 0.5mg/L, adding a hatching agent for hatching, wherein the addition amount of the hatching agent is 7 percent of the weight of the shrimp, the hatching agent is thyroxine, epinephrine and soybean isoflavone in a volume ratio of 18;
s3, larva cultivation: feeding the larva into a nursery pond with a cultivation density of 12 × 10 4 Tail/m 3 Culturing water at 30 ℃, pH 8.0, dissolved oxygen above 5mg/L, ammonia nitrogen below 0.1mg/L, nitrite nitrogen below 0.05mg/L and salinity below 30 per mill, and removing the shrimp from the culturing pool after culturing the shrimp into shrimp.
Comparative example 1
The difference between the comparative example and the example 3 is that the strengthening solution in S2 is povidone-iodine, potassium permanganate and benzoyl peroxide in a volume ratio of 8: the method comprises the following steps:
s1, setting an environment: arranging a hatching pond, adding 7mg/L of sodium acetate into the hatching pond with the water depth of 1m, and airing for 3d for later use;
s2, hatching fertilized eggs: and (2) soaking the fertilized eggs in a strengthening solution, wherein the strengthening solution is povidone iodine, potassium permanganate and benzoyl peroxide in a volume ratio of 8 2 Ten thousand per m 3 Transferring the shrimp to the hatching pond after immersion bath, setting the water temperature of the hatching pond to be 30 ℃, the water quality and the salinity to be 25 per mill, the dissolved oxygen to be 6mg/L, the illumination intensity to be 30Lx and the ammonia nitrogen to be less than 0.5mg/L, putting a hatching agent into the hatching pond for hatching, wherein the addition amount of the hatching agent is 5-8% of the weight of the shrimp, the hatching agent is thyroxine, epinephrine and soybean isoflavone with the volume ratio of 118;
s3, larva cultivation: feeding the larva into a nursery pond with a cultivation density of 12 × 10 4 Tail/m 3 Culturing water at 30 ℃, pH 8.0, dissolved oxygen above 5mg/L, ammonia nitrogen below 0.1mg/L, nitrite nitrogen below 0.05mg/L and salinity below 30 per mill, and removing the shrimp from the culturing pool after culturing the shrimp into shrimp.
Comparative example 2
The difference between the comparative example and the example 3 is that the hatching agent of S2 is thyroxine, epinephrine and soybean isoflavone with the volume ratio of 10: the method comprises the following steps:
s1, setting an environment: arranging a hatching pond, adding 7mg/L of sodium acetate into the hatching pond with the water depth of 1m, and airing for 3d for later use;
s2, hatching fertilized eggs: soaking fertilized eggs in a strengthening solution, wherein the strengthening solution is prepared from the following components in a volume ratio of (4):2, the mass concentration of the povidone iodine is 8mg/L, the mass concentration of the potassium permanganate is 15mg/L, the mass concentration of the benzoyl peroxide is 5mg/L, the bathing temperature is 25 ℃, the bathing time is 30 hours, and the soaking density of the fertilized eggs is 10 multiplied by 10 2 Ten thousand per m 3 Transferring the shrimp into the hatching pond after the dipping bath is finished, setting the water temperature of the hatching pond to be 30 ℃, the salinity of water to be 25 per mill, the dissolved oxygen to be 6mg/L, the illumination intensity to be 30Lx and the ammonia nitrogen to be less than 0.5mg/L, adding a hatching agent for hatching, wherein the addition amount of the hatching agent is 7 percent of the weight of the shrimp, the hatching agent is thyroxine, epinephrine and soybean isoflavone in a volume ratio of 10;
s3, larva cultivation: feeding the larva into a nursery pond with a cultivation density of 12 × 10 4 Tail/m 3 Culturing water at 30 ℃, pH 8.0, dissolved oxygen above 5mg/L, ammonia nitrogen below 0.1mg/L, nitrite nitrogen below 0.05mg/L and salinity below 30 per mill, and removing the shrimp into a culture pond to culture the shrimp.
Comparative example 3
The difference between the comparative example and the example 3 is that the strengthening solution is povidone iodine and potassium permanganate with the volume ratio of 4: the method comprises the following steps:
s1, setting an environment: arranging a hatching pond, adding 7mg/L of sodium acetate into the hatching pond with the water depth of 1m, and airing for 3d for later use;
s2, hatching fertilized eggs: soaking the fertilized eggs in a strengthening solution, wherein the strengthening solution is povidone iodine and potassium permanganate with the volume ratio of 4 to 3, the mass concentration of the povidone iodine is 8mg/L, the mass concentration of the potassium permanganate is 15mg/L, the mass concentration of benzoyl peroxide is 5mg/L, the soaking temperature is 25 ℃, the soaking time is 30h, and the soaking density of the fertilized eggs is 10 multiplied by 10 2 Ten thousand per m 3 And after bathing, transferring the shrimp into the hatching pond, setting the water temperature of the hatching pond to be 30 ℃, the salinity of water to be 25 per mill, the dissolved oxygen to be 6mg/L, the illumination intensity to be 30Lx and the ammonia nitrogen to be less than 0.5mg/L, and adding a hatching agent to hatch, wherein the adding amount of the hatching agent is 7 percent of the weight of the shrimp, and the hatching agent is 18 percent by volumeStirring the water in the pool by an egg stirrer every 1-2 h, and checking the development condition of the embryo at regular time until the fertilized egg develops into a larva;
s3, larva cultivation: feeding the larva into a nursery pond with a cultivation density of 12 × 10 4 Tail/m 3 Culturing water at 30 ℃, pH 8.0, dissolved oxygen above 5mg/L, ammonia nitrogen below 0.1mg/L, nitrite nitrogen below 0.05mg/L and salinity below 30 per mill, and removing the shrimp into a culture pond to culture the shrimp.
Comparative example 4
The difference between the comparative example and the example 3 is that the hatching agents are thyroxine and epinephrine with the volume ratio of 18: the method comprises the following steps:
s1, setting an environment: arranging a hatching pond, adding 7mg/L of sodium acetate into the hatching pond with the water depth of 1m, and airing for 3d for later use;
s2, hatching fertilized eggs: and (2) soaking the fertilized eggs in a strengthening solution, wherein the strengthening solution is povidone iodine, potassium permanganate and benzoyl peroxide in a volume ratio of 4 2 Ten thousand per m 3 Transferring the shrimp to the hatching pond after immersion bath, setting the water temperature of the hatching pond to be 30 ℃, the water quality and the salinity to be 25 per mill, the dissolved oxygen to be 6mg/L, the illumination intensity to be 30Lx and the ammonia nitrogen to be less than 0.5mg/L, putting a hatching agent into the hatching pond for hatching, wherein the adding amount of the hatching agent is 7 percent of the weight of the shrimp, the hatching agent is thyroxine, epinephrine and soybean isoflavone with the volume ratio of 18;
s3, larva cultivation: feeding the larva into a nursery pond with a cultivation density of 12 × 10 4 Tail/m 3 Culturing water at 30 ℃, pH 8.0, dissolved oxygen above 5mg/L, ammonia nitrogen below 0.1mg/L, nitrite nitrogen below 0.05mg/L and salinity below 30 per mill, and removing the shrimp from the culturing pool after culturing the shrimp into shrimp.
1. Index measurement
Incubation time: 50% of the fertilized eggs in the same batch are hatched to form membranes
Hatchability/% = (number of hatched young shrimps/number of fertilized eggs) × 100%
Deformity rate/% = (number of deformed young shrimps/number of hatched young shrimps) × 100%
The results of the measurement were as follows:
the results show that the hatching method can improve the hatching rate of the prawns and reduce the teratogenesis rate of fertilized eggs, and the comparative example 1 shows that the strengthening liquid prevents various diseases of the prawns to a great extent in bath under a certain proportion, prevents vertical transmission of pathogens and has obvious effect of clearing bacteria attached to the fertilized eggs; compared with the comparative example 2, the hatching agent and the hatching method can effectively improve the hatching rate of fertilized eggs of the prawns to 98.2 percent, the larva aberration rate is only 1.3 percent, and the healthy and qualified prawns can be cultured.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (6)
1. A prawn larva incubation method is characterized in that: the method comprises the following steps:
s1, setting an environment: arranging an incubation pool, wherein the water depth is 0.7-1.2 m, adding 5-8 mg/L of sodium acetate, and airing for 2-3 d for later use;
s2, hatching fertilized eggs: soaking the fertilized eggs in a strengthening solution, wherein the strengthening solution is povidone iodine, potassium permanganate and benzoyl peroxide in a volume ratio of 3-5 2 Ten thousands per liter, transferring the fertilized eggs into the hatching pond after the dipping bath is finished, setting the water temperature of the hatching pond to be 28-35 ℃, the salinity of the water to be 20-30 per thousand, the dissolved oxygen to be 5-7 mg/L, the illumination intensity to be 20-40 Lx and the ammonia nitrogen to be less than 0.5mg/L, putting a hatching agent into the hatching pond for hatching, stirring the pond water by an egg stirrer every 1-2 hours, and regularly checking the development condition of the embryos until the fertilized eggs develop into young bodies;
s3, larva cultivation: feeding the larva into a seedling raising pond, wherein the culture density is (10-14) multiplied by 10 4 Tail/m 3 The water temperature for cultivation is 28-32 ℃, the pH value is 7.8-8.4, the dissolved oxygen is more than 5mg/L, the ammonia nitrogen is less than 0.1mg/L, the nitrite nitrogen is less than 0.05mg/L, and the salinity is 26-35 per mill.
2. The method for incubating prawn larvae according to claim 1, wherein the method comprises the following steps: the mass concentration of the povidone iodine is 5-10 mg/L, the mass concentration of the potassium permanganate is 10-20 mg/L, and the mass concentration of the benzoyl peroxide is 3-8 mg/L.
3. The method for incubating the larvae of prawns according to claim 1, wherein the steps of: the strengthening solution is povidone iodine, potassium permanganate and benzoyl peroxide in a volume ratio of 4.
4. The method for incubating the larvae of prawns according to claim 1, wherein the steps of: the hatching agent is thyroxine, adrenaline and soybean isoflavone with the volume ratio of 15-20.
5. The method for incubating prawn larvae according to claim 1, wherein the method comprises the following steps: the hatching agent comprises thyroxine, adrenaline and soybean isoflavone with the volume ratio of 18.
6. The method for incubating prawn larvae according to claim 1, wherein the method comprises the following steps: the addition amount of the hatching agent is 5-8% of the weight of the shrimps.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050238688A1 (en) * | 2001-03-21 | 2005-10-27 | Dean Klein | Method of preparing an immunologically inert graft material from body tissue and material made with the method |
| US20090274770A1 (en) * | 2006-05-11 | 2009-11-05 | Regenics As | Cellular extracts |
| CN107079908A (en) * | 2017-06-02 | 2017-08-22 | 兴化市恒威生物技术有限公司 | A kind of aquatic products thimerosal and preparation method thereof |
| CN108834960A (en) * | 2018-08-07 | 2018-11-20 | 海南中正水产科技有限公司 | A kind of high yield incubation method of Penaeus Vannmei nauplius |
| CN110150195A (en) * | 2019-03-29 | 2019-08-23 | 天津市水产研究所 | A kind of incubation method of the litopenaeus vannamei young |
-
2022
- 2022-08-26 CN CN202211037503.7A patent/CN115380857A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050238688A1 (en) * | 2001-03-21 | 2005-10-27 | Dean Klein | Method of preparing an immunologically inert graft material from body tissue and material made with the method |
| US20090274770A1 (en) * | 2006-05-11 | 2009-11-05 | Regenics As | Cellular extracts |
| CN107079908A (en) * | 2017-06-02 | 2017-08-22 | 兴化市恒威生物技术有限公司 | A kind of aquatic products thimerosal and preparation method thereof |
| CN108834960A (en) * | 2018-08-07 | 2018-11-20 | 海南中正水产科技有限公司 | A kind of high yield incubation method of Penaeus Vannmei nauplius |
| CN110150195A (en) * | 2019-03-29 | 2019-08-23 | 天津市水产研究所 | A kind of incubation method of the litopenaeus vannamei young |
Non-Patent Citations (1)
| Title |
|---|
| 孙翰昌: "《现代水产养殖学的技术创新研究》", 长春：东北师范大学出版社, pages: 72 - 72 * |
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