CN115380854A - Method for obtaining egg-carrying shrimps in batches - Google Patents

Method for obtaining egg-carrying shrimps in batches Download PDF

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CN115380854A
CN115380854A CN202210857854.6A CN202210857854A CN115380854A CN 115380854 A CN115380854 A CN 115380854A CN 202210857854 A CN202210857854 A CN 202210857854A CN 115380854 A CN115380854 A CN 115380854A
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shrimps
water
egg
female
carrying
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王大鹏
陆专灵
黄彬胜
唐章生
曾华忠
黄博
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Guangxi Academy of Fishery Sciences
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00Culture of aquatic animals
    • A01K61/50Culture of aquatic animals of shellfish
    • A01K61/59Culture of aquatic animals of shellfish of crustaceans, e.g. lobsters or shrimps
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Zoology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Farming Of Fish And Shellfish (AREA)

Abstract

The invention provides a method for obtaining egg-carrying shrimps in batches, and belongs to the technical field of aquaculture. A method for obtaining oviparous shrimps in batches is characterized in that the height of a ridge of a rice field is set to be more than 50cm, a cuboid soil pit is arranged in the middle of the rice field, meanwhile, an independent water inlet pipe and an independent water outlet pipe are arranged in the rice field, and an outdoor cultivation pool is obtained through transformation; feeding seed shrimps after water is fed into the outdoor cultivation pool, checking the development condition of the female shrimps after the cultivation for 3 days, and draining water and drying the field when more than 60% of the female shrimps develop to the gonad III stage; and on the 10 th day of sunning, feeding water into the outdoor culture pond again, sorting female shrimps, collecting the female shrimps with eggs on the abdomens, and culturing the female shrimps without eggs on the abdomens by parent shrimps to obtain the egg-carrying shrimps. The method provided by the invention can stably obtain the egg-carrying shrimps in batches, and provides rich material sources for industrial seedling culture.

Description

Method for obtaining egg-carrying shrimps in batches
Technical Field
The invention belongs to the technical field of aquaculture, and particularly relates to a method for obtaining egg-carrying shrimps in batches.
Background
The procambarus clarkii is commonly called crayfish, the mainstream breeding mode of the procambarus clarkii is still a semi-artificial breeding mode, namely, the seed shrimps are selected and reserved in the rice field or the pond, the seed shrimps naturally mate and breed after sexual maturity, the offspring seeds grow to be above 3g and are caught by a ground cage, the offspring seeds cannot be completely caught by the ground cage catching method because the breeding of the seed shrimps is asynchronous, and the rest offspring seeds are reserved in the rice field or the pond for continuous breeding after the peak period of emergence. At present, a breeding and separating mode mainly developed is to select a part of rice fields or ponds as special breeding ponds, but the essence of the mode is still a semi-artificial breeding mode, and the quantity of sexually mature parent shrimps and the quantity of seedlings in the ponds cannot be accurately estimated. The most important problem is that the seedlings bred in the rice field or the pond can be conveniently caught in the ground cages in batches only when the weight of the seedlings reaches 3g in the original pond, and because the density of the seedling breeding pond is higher, the growth speed of the seedlings is slow, and the survival rate is lower.
The Guangxi rice and shrimp breeding is rapidly developed in nearly two years, particularly in a winter fallow field breeding mode, only ridges need to be heightened, surrounding ditches do not need to be excavated, the land circulation is easy, no conflict with rice planting exists, the crayfishes bred in the winter fallow field are listed from spring to Qingming festival, and the sale price of the general goods is more than 1 time higher than that of the main production area in the middle and lower reaches of the Yangtze river. The current problems are that the time of breeding shrimp seedlings in the rice field in Guangxi province is mainly from 11 late months to 2 middle and late months, only 1 crayfish can be bred, and the crayfish on the market in spring festival has small specification and is generally below six money. If the putting time of the offspring seeds can be advanced to the last ten days of 10 months, the temperature advantage between 10 months and 12 months is fully utilized, the market-selling specification and the yield of the spring crayfish can be effectively improved, and even 2 crayfish are bred in the slack winter period. The industrial seedling raising has the advantages of early seedling emergence and small seedling specification. The industrial seedling raising and seedling emergence time is from the first 10 months to the later 11 months generally due to the limitation of egg-carrying shrimp supply, because the shrimp seedlings can emerge when the specification is below 1g, and the method has no influence of enemy harm and high survival rate of the seedlings, generally, more than 400 shrimp seedlings can be produced by each female shrimp, and in a paddy field, only 20-50 shrimp seedlings can be produced by each female shrimp on average. The crayfish fry hatching has low requirements on the environment, the suitable temperature range is wide, and only about 20 days are needed from the time when the female crayfish spawns to the time when the crayfish hatches. Few enemies are bred in slack winter fields, the requirement on the specification of offspring seeds leaving the pond is not high, and the method is suitable for throwing small-specification offspring seeds. Therefore, the industrial seedling culture is a necessary means for guaranteeing the supply of the seedlings cultured in the slack winter field, which is a great possibility in Guangxi province.
The industrial breeding of the crayfish is already researched in Hubei, jiangsu and other places, but the industrial breeding of the crayfish is not applied much in production, the main bottleneck problem lies in the supply of the egg-carrying crayfish, the egg-carrying crayfish is directly cultivated in a workshop, more space is occupied, the spawning is slow, the spawning is asynchronous, and the death rate is very high. And the oogenesis shrimps are caught from the rice field or the pond, because the oogenesis shrimps move less and are not easy to enter the cage, the obtaining of the oogenesis shrimps nearby is difficult, and the oogenesis shrimps leave water for more than 1 hour, so that the hatchability of fertilized eggs is greatly reduced. In recent years, by utilizing the advantage of large local population scale, the egg-carrying shrimps are selected at the sales points, fertilized eggs are stripped for in vitro incubation, female shrimps are sold to reduce the cost, most of the egg-carrying shrimps at the sales points leave water for more than 1 hour, the in vitro incubation further reduces the incubation rate, and the problem of unstable supply of the egg-carrying shrimps always exists. Therefore, how to obtain the egg-carrying shrimps in batches in the seedling raising workshop is an important problem which needs to be solved urgently in industrial seedling raising.
Disclosure of Invention
In view of this, the invention aims to provide a method for obtaining egg-carrying shrimps in batches, which provides abundant egg-carrying shrimps for industrial seedling culture.
The invention provides a method for obtaining egg-carrying shrimps in batches, which comprises the following steps:
1) Setting the height of a ridge of a rice field to be more than 50cm, arranging a cuboid soil pit in the middle of the rice field, and arranging independent water inlet and outlet pipes in the rice field to obtain an outdoor cultivation pond;
2) Feeding seed shrimps into the outdoor cultivation pond in the step 1) after water is fed, checking the development condition of female shrimps after 3 days of cultivation, and draining water and drying the field when more than 60% of female shrimps develop to the gonad III stage;
3) And (4) feeding water into the outdoor culture pond again on the 10 th day of sunning, sorting female shrimps, collecting the female shrimps with the abdominal eggs, and culturing the female shrimps without the abdominal eggs to obtain the egg-carrying shrimps.
Preferably, the area of the rice field in the step 1) is 1-2 mu.
Preferably, a circle of shielding objects which are at least 40cm higher than the field surface are arranged at the base part of the inner side of the ridge in the step 1).
Preferably, the shelter is a fence made of a plastic film and bamboo chips.
Preferably, the soil pit in the step 1) has the specification of 9-11 m in length, 5-7 m in width and 15-25 cm in depth.
Preferably, the water inlet height of the outdoor culture pond in the step 1) is 25-35 cm;
the feeding density of the seed shrimps is 50 kg/mu.
Preferably, the water draining method in the step 2) is to drain the water in the outdoor culture pond within 11-13 h.
Preferably, the height of the water re-entering in the step 3) is 25-35 cm.
Preferably, the female shrimps are sorted in the step 3) 2 days after the water is again taken.
Preferably, the method for breeding the parent shrimps in the step 3) is 0: 00-21, culturing the micro flowing water by using an outdoor pond water source, and culturing the micro flowing water by using a deep well low-temperature water source in a period of 21;
the flow rate of the micro-flowing water culture is 8-10L/min, and the water exchange rate per hour is 50-60%. The invention provides a method for obtaining ovigerous shrimps in batches, which is characterized in that the ovigerous shrimps are cultivated by combining outdoor cultivation and indoor cultivation, specifically, a paddy field is transformed into an outdoor cultivation pool, then the breeding shrimps are put in the outdoor cultivation pool, water is drained when the breeding shrimps grow to gonad III stage, the crayfishes are drilled into field soil, then the female shrimps are sorted by return water, the female shrimps with ova already on the belly are collected, and the female shrimps without ova on the belly are promoted to grow and mature under indoor conditions, so that a large number of ovigerous shrimps are obtained. The method provided by the invention provides abundant parent materials for crayfish breeding, and greatly promotes the development of crayfish breeding industry. Meanwhile, the method provided by the invention has the characteristics of simple and convenient operation, short period and good effect, and is suitable for popularization and application in the crayfish breeding industry.
Drawings
FIG. 1 is a graph of the appearance of the ovary of procambarus clarkii at 4 stages of development, wherein A is stage I appearance, B is stage II appearance, C is stage III appearance, and D is stage IV appearance.
Detailed Description
The invention provides a method for obtaining egg-carrying shrimps in batches, which comprises the following steps:
1) Setting the height of a ridge of a rice field to be more than 50cm, arranging a cuboid soil pit in the middle of the rice field, and arranging independent water inlet and outlet pipes in the rice field to obtain an outdoor cultivation pond;
2) Feeding seed shrimps into the outdoor cultivation pond in the step 1) after water is fed, checking the development condition of female shrimps after 3 days of cultivation, and draining water and drying the field when more than 60% of female shrimps develop to the gonad III stage;
3) And (3) feeding water into the outdoor cultivation pond again on the 10 th day of the field, sorting female shrimps, collecting the female shrimps with the abdominal eggs, and cultivating the female shrimps without the abdominal eggs through parent shrimps to obtain the egg-carrying shrimps.
The method comprises the steps of setting the height of a ridge of a rice field to be more than 50cm, arranging a cuboid soil pit in the middle of the rice field, and arranging independent water inlet and outlet pipes in the rice field to obtain an outdoor cultivation pond.
In the present invention, the area of the paddy field is preferably 1 to 2 acres. The height of the ridge of the rice field is preferably 55-60 cm. A circle of shielding objects which are at least 40cm higher than the field surface are preferably arranged at the base part of the inner side of the ridge. The shelter is preferably a fence made of plastic film and bamboo chips. The shelter prevents crayfish from digging a hole on the ridge to escape.
In the present invention, the soil pit preferably has a length of 9 to 11m, a width of 5 to 7m, and a depth of 15 to 25cm, more preferably a length of 10m, a width of 6m, and a depth of 20cm. The soil pit is used for catching shrimps in a cage and facilitating water drainage, and the drain pipe is connected to the soil pit to well prevent escape.
In the invention, the independent water inlet and outlet pipes are arranged in the rice field, and the water inlet height of 30cm and the water draining time are controlled within 12h each time.
After the outdoor cultivation pool is obtained, the method comprises the steps of feeding water into the outdoor cultivation pool, then putting the seed shrimps, cultivating for 3 days, then checking the development condition of the female shrimps, and draining water and drying the field when more than 60% of the female shrimps develop to the gonad III stage.
In the invention, the outdoor cultivation pond is preferably only planted with middle rice of 1 season, and water is fed after the rice is harvested in the middle ten 8 months. In Guangxi, the female shrimps which grow to the gonad III stage are more than 60% before 8 months.
In the present invention, the height of the inlet water of the outdoor rearing tank is preferably 25 to 35cm, more preferably 30cm. The feeding density of the seed shrimps is preferably 50 kg/mu. The draining method is preferably to drain the water in the outdoor culture pond within 11-13 h. Draining water to simulate adverse environment, and promoting crayfish to make hole in the field and enter mud to accelerate the reproductive development process. If there are more water birds such as aigrette in the area, attention is paid to driving or bird prevention nets are arranged.
On the 10 th day of the field, the outdoor cultivation pond is filled with water again, female shrimps are sorted out, the female shrimps with eggs on the abdomens are collected, and the female shrimps without eggs on the abdomens are cultivated to obtain the egg-carrying shrimps through parent shrimps.
In the present invention, the height of the re-entry of water is preferably 25 to 35cm, more preferably 30cm. The time for sorting out female shrimps is preferably 2 days after the water is again introduced. A method for sorting female shrimps adopts a ground cage to catch crayfishes. The obtained male shrimps are sold. The obtained female shrimps with the abdomen already carrying eggs are placed in a breeding pond for laying eggs. The method for judging the female shrimp whose abdomen is laid with eggs is preferably that the female shrimp whose abdomen is laid with eggs is observed when the tail of the female shrimp is curled. The method for judging the female lobster with no egg-carrying in the abdomen is preferably to observe that the tail of the female lobster does not curl, which indicates that the abdomen does not carry the egg.
In the present invention, the method for breeding parent shrimps is preferably 0: 00-21, culturing the micro flowing water by using an outdoor pond water source, and culturing the micro flowing water by using a deep well low-temperature water source in a period of 21. The flow rate of the micro-flow water culture is preferably 8 to 10L/min, and more preferably 9L/min. The water exchange rate per hour is preferably 50% to 60%, more preferably 55%. The water temperature of the outdoor pond water source is consistent with the ambient temperature, and is 28-34 ℃. The water temperature of the deep well low-temperature water source is preferably not higher than 15 ℃. When the water temperature of the deep well low-temperature water source is higher than 15 ℃, a cooling device is arranged for cooling. The breeding density of the parent shrimp is preferably 40/m 2 . What is needed isThe parent shrimps were not fed with the feed during the cultivation period.
In the present invention, 80% of female shrimps can lay eggs after 7 days of parent shrimp cultivation. On the 3 rd day of parent shrimp culture, the egg-carrying shrimps were selected once a day. Preferably, the water level of the ripening pond is reduced to 5cm, the bent tail of the shrimp is observed, and the shrimp which cannot see the tail handle is the egg-carrying shrimp which is picked up for later use.
The method provided by the present invention is suitable for procambarus clarkii only, and the embodiment of the present invention will be specifically described with procambarus clarkii as a breeding target. The method can obtain more than 30 kg/mu of egg-carrying shrimps in batches within 20 days by calculating that 50kg of shrimp seeds are put into each mu of outdoor culture pond, the putting amount is more than 60%, for example, the obtained egg-carrying shrimps account for more than 75% by calculating that the female shrimps are about 40 kg/mu when put. The method for directly catching and sorting the egg-carrying shrimps by normally using the ground cages not only has large workload, but also can only obtain less than 10kg of egg-carrying shrimps per mu in the production period of two months on averageLess than 20% of the amount of the feed
The method for obtaining egg-carrying shrimps in batches provided by the invention is described in detail below with reference to the examples, but the method is not to be construed as limiting the scope of the invention.
Example 1
A method for obtaining egg-carrying shrimps in batches comprises the following steps:
1. construction of outdoor cultivation pool
The outdoor cultivation pond is obtained by modifying a rice field, the area of the outdoor cultivation pond is 1.5 mu, ridges are heightened to 50cm, and the width of the outdoor cultivation pond is 8 meters. The water inlet and the water outlet are independently arranged, and the time for each water inlet of 30cm and the time for draining water are controlled within 12 h. A circle of enclosure is bound up by using a plastic film and bamboo chips along the base part of the inner side of the ridge and is 40cm higher than the field surface. A soil pit with the length of 10 meters, the width of 6 meters and the depth of 20cm is dug in the middle of the cultivation pool and used for catching shrimps in a cage and facilitating drainage, and a drainage pipe is connected to the soil pit.
2. Throwing in spare seed shrimps
Only planting middle rice in 1 season in an outdoor cultivation pond, after harvesting the rice in 8 middle ten days, feeding water with the height of 30cm, putting seed shrimps according to the density of 50kg per mu, after adapting to 3 days, manually throwing cages to catch the shrimps, and checking the gonad development degree of the female shrimps. When more than 60% of the female shrimps developed to gonad III stage (see C in figure 1) by spot inspection, drainage and field drying are started. Before 8 months in Guangxi, the female shrimps which grow to the gonad III stage all exceed 60 percent.
3. Drainage sunning field
Slowly draining water, controlling the water draining time to be about 12h, forcing the crayfish to make a hole on the field surface, and drying the field for 10 days from the water draining. When there are more water birds such as aigrette in the area, a bird-preventing net should be arranged to prevent the water birds from pecking crayfish.
4. Backwater shrimp catching
After the field is sunned for 10 days, water is fed into the outdoor cultivation pond to reach the height of 30cm, crayfish is caught by a ground cage after two days, the caught crayfish is sorted, male crayfish is sold, female crayfish enters a seedling raising workshop and is divided into two types after entering the seedling raising workshop, one type is that the belly of the crayfish is already laid with eggs, the crayfish directly enters the seedling raising pond, and the belly of the crayfish is not laid with eggs and is placed into the indoor parent crayfish cultivation pond.
5. Indoor ripening
The indoor parent shrimp culture pond is in a strip shape, 2 meters wide and 6-10 meters long. The water inlet and the water outlet are respectively arranged at the two ends of the pool. The water inlet source is provided with two water sources, one is outdoor pond water, and the water temperature is kept consistent with the external environment water temperature (28-34 ℃). One is deep well low temperature water, the water temperature is controlled below 15 ℃, if the water temperature is higher than 15 ℃, a cooling device is arranged to cool the water to below 15 ℃.
40 female shrimps without eggs on the abdomen are thrown according to the density of each square meter, and feeding is not carried out in the ripening acceleration process. 0: 00-21, using an outdoor pond water source, adopting micro flowing water (the flow rate is 9L/min, the water exchange rate is 55%/h), and using a deep well low-temperature water source for the 00-24. From the 3 rd day, egg-carrying female shrimps are selected once a day, the water level of the ripening pond is reduced to 5cm, the shrimps are observed from the upper part, the tail parts of the shrimps are bent, and the shrimps with the tail handles which cannot be seen are egg-carrying shrimps, and the shrimps are picked up by a manual picking mode and are placed into a hatching pond. More than 80% of female shrimps can lay eggs within 7 days of ripening to obtain a large amount of egg-carrying shrimps.
The female shrimp seeds put by the method can successfully recover more than 85 percent of the egg-carrying shrimps. Factory seedling raising experiments show that compared with the egg-carrying shrimps directly captured in the rice field or the pond, the egg-carrying shrimps ripened by the method avoid egg drop caused by scraping of a ground cage in the capturing process, the egg-carrying amount per unit weight is more than 12 eggs/g weight, the average value in production is more than 10 eggs/g weight, the hatching rate is normal, and the phenomena of abnormal eggs and the like are not found.
Comparative example 1
The oviparous shrimp rearing was carried out in accordance with the method of example 1, except that the field was sunned for 5 days. The caught crayfishes were sorted, and the egg-carrying shrimps were not collected to obtain a large number of female shrimps which were not egg-carrying, and parent shrimp breeding was performed according to the method of example 1, and after 7 days of breeding, only 30% of the female shrimps were egg-carrying shrimps. The proportion of the egg-carrying shrimps in the fed female shrimps is only 37-40% by weight.
Comparative example 2
The incubation of oviparous shrimps was carried out according to the method of example 1, except that 14 days were exposed to the sun, and only a very small amount of female shrimps were obtained after catching crayfish with the ground cage. The analysis reason may be that the field drying time is too long, the gonads of the female shrimps are developed into the V-th stage and are ready to lay eggs, the female shrimps basically do not climb at the stage, and the ground cages cannot capture a large amount of egg-carrying shrimps.
Comparative example 3
The method of example 1 was followed for the incubation of oviparous shrimp, except that only the outside pond water source was used for the micro-flow incubation during the ripening process. The result shows that only 10-15% of female shrimps can lay eggs within 7 days of ripening, and only 40-50% of female shrimps can lay eggs within 30 days of ripening.
Comparative example 4
The incubation of the oviparous shrimps was carried out according to the method of example 1, except that the temperature of the deep well low temperature water source was controlled at 17 ℃ during the ripening process. The results show that less than 5% of the female shrimps can lay eggs within 7 days of ripening, and only 10-15% of the female shrimps can lay eggs within 10 days of ripening. Only 40-50% of female shrimps can lay eggs after 30 days of ripening.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.

Claims (10)

1. A method for obtaining egg-carrying shrimps in batches is characterized by comprising the following steps:
1) Setting the ridge height of a rice field to be more than 50cm, arranging a cuboid soil pit in the middle of the rice field, and arranging independent water inlet and outlet pipes in the rice field to obtain an outdoor cultivation pond;
2) Feeding seed shrimps into the outdoor cultivation pond in the step 1) after water is fed, checking the development condition of female shrimps after 3 days of cultivation, and draining water and drying the field when more than 60% of female shrimps develop to the gonad III stage;
3) And (3) feeding water into the outdoor cultivation pond again on the 10 th day of the field, sorting female shrimps, collecting the female shrimps with the abdominal eggs, and cultivating the female shrimps without the abdominal eggs through parent shrimps to obtain the egg-carrying shrimps.
2. The method for obtaining the egg-carrying shrimps in batches according to claim 1, wherein the area of the rice field in the step 1) is 1-2 mu.
3. The method for obtaining the oviparous shrimps in batches according to claim 1, wherein a circle of shelter which is at least 40cm higher than the field surface is arranged at the base part of the inner side of the ridge in the step 1).
4. The method for obtaining the ovigerous shrimps in batches according to claim 3, wherein the shielding object is a fence made of a plastic film and bamboo chips.
5. The method for obtaining the oviferous shrimps in batches according to claim 1, wherein the specifications of the soil pits in the step 1) are 9-11 m in length, 5-7 m in width and 15-25 cm in depth.
6. The method for obtaining the egg-carrying shrimps in batches according to claim 1, wherein the height of the inlet water of the outdoor culture pond in the step 1) is 25-35 cm;
the feeding density of the seed shrimps is 50 kg/mu.
7. The method for obtaining the egg-carrying shrimps in batches according to claim 1, wherein the water in the step 2) is drained by draining the outdoor culture pond for 11-13 h.
8. The method for obtaining the egg-carrying shrimps in batches according to claim 1, wherein the height of the re-feeding water in the step 3) is 25-35 cm.
9. The method for obtaining the egg-carrying shrimps in batches according to claim 1, wherein the time for sorting the female shrimps in the step 3) is 2 days after water is again fed.
10. The method for obtaining the egg-carrying shrimps in batches according to any one of claims 1 to 9, wherein the method for breeding the parent shrimps in the step 3) is 0: 00-21, culturing the micro flowing water by using an outdoor pond water source, and culturing the micro flowing water by using a deep well low-temperature water source in a period of 21;
the flow rate of the micro-flowing water culture is 8-10L/min, and the water exchange rate per hour is 50-60%.
CN202210857854.6A 2022-07-20 2022-07-20 Method for obtaining egg-carrying shrimps in batches Pending CN115380854A (en)

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Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110537512A (en) * 2019-09-20 2019-12-06 江苏省淡水水产研究所 Method for 'autumn seedling early propagation' of procambarus clarkii
CN110881432A (en) * 2019-12-20 2020-03-17 浙江大学 Ecological breeding method for rice field crayfishes

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110537512A (en) * 2019-09-20 2019-12-06 江苏省淡水水产研究所 Method for 'autumn seedling early propagation' of procambarus clarkii
CN110881432A (en) * 2019-12-20 2020-03-17 浙江大学 Ecological breeding method for rice field crayfishes

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
唐建清 等: "《淡水小龙虾高效生态养殖新技术》", 中国农业出版社, pages: 118 - 120 *

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