CN115363056A - Preparation method and application of cyathula officinalis leaching liquor for preventing and treating crop bacterial wilt - Google Patents
Preparation method and application of cyathula officinalis leaching liquor for preventing and treating crop bacterial wilt Download PDFInfo
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Abstract
The invention discloses a preparation method and application of an echinacea purpurea leaching liquor for preventing and treating crop bacterial wilt, wherein the echinacea purpurea is mixed with a phosphate buffer solution according to the proportion of 10 mL/g, and is leached for 2d at normal temperature to obtain the echinacea purpurea leaching liquor. The pot control effect is measured by adopting a leaching liquor root irrigation method, and experimental results prove that after 21 d of inoculation, the control effect of the cyathula officinalis leaching liquor on tobacco bacterial wilt is 76.08% by adopting the cyathula officinalis leaching liquor for treatment, and the obtained cyathula officinalis leaching liquor has a good control effect on crop bacterial wilt.
Description
Technical Field
The invention belongs to the technical field of biological control of plant diseases, and particularly relates to a preparation method and application of a achyranthes bidentata chrysanthemum leaching liquor for controlling crop bacterial wilt.
Background
Crop bacterial wilt is a typical soil-borne disease, and the diseaseThe pathogenic substance of (A) is Qing Ralstonia solanacearum: (B)Ralstonia solanacearum) It has wide host range, such as eggplant, tomato, chili, potato, peanut, etc. The ralstonia solanacearum can be spread through soil and irrigation water, invade from the root of a host plant, enter xylem vascular bundles, and rapidly spread to overground tissues of the plant through a vascular bundle system, and typical symptoms of diseases are that xylem is browned and the plant is wilted. The occurrence of bacterial wilt in a large area causes the quality and yield of agricultural products to be seriously affected, causes irreparable economic loss and is one of the most destructive plant bacterial diseases in tropical, subtropical and temperate regions.
The control strategy of crop withering is mainly characterized by that it uses disease-resistant cultivation as basis, uses disease-resistant variety as centre and uses chemical control as auxiliary measure, and its control measures mainly include selection of disease-resistant high-quality variety or grafted seedling, rotation of disease-resistant variety, cultivation of healthy strong seedling with disease-free new soil, improvement of soil, timely removal of diseased plant and application of pesticide. However, the large and frequent use of chemical pesticides threatens the sustainability of ecology and the health of human beings, and therefore, the development of "green agriculture" is imminent.
In recent years, biological control has become a focus of research. Since plants are rich in active substances and beneficial microorganisms, which have natural defense effects against diseases and pests, the prevention and control of diseases by using plant-derived extracts is one of the biological control methods. The plant extract has natural materials, wide sources and complex components, is not easy to generate drug resistance in the process of preventing and treating plant diseases and insect pests, and has no residue on the environment and crops. Therefore, the development and utilization of abundant plant resources in China have profound significance in pest control.
Achyranthes bidentata Ju (Galinsogaparviflora) Belongs to an exotic invasive weed and poses a threat to local species. The acetone extract of the achyranthes bidentata blume is used for inhibiting plant pathogenic fungi such as bakanae disease bacteria and rice blast bacteria in the prior report, but does not relate to the plant pathogenic bacteria, and the acetone extract is extracted by adopting an organic solvent, so that the environmental pollution is easily caused.
Disclosure of Invention
The invention aims to provide a preparation method and application of a achyranthes bidentata chrysanthemum leaching liquor for preventing and treating crop bacterial wilt. The achyranthes bidentata leaching liquor is prepared and applied to the prevention and control of the crop bacterial wilt, so that the external invasive plant achyranthes bidentata can be consumed, and the achyranthes bidentata leaching liquor can be used for preventing and controlling the crop bacterial wilt, thereby having better application prospect.
In order to achieve the purpose, the invention adopts the following technical scheme:
a preparation method of an achyranthes bidentata chrysanthemum leaching liquor for preventing and treating crop bacterial wilt comprises the steps of cutting fresh achyranthes bidentata chrysanthemum into small sections of 2-5cm, adding phosphate buffer solution with the pH =7 and the concentration of 0.20moL/mL according to the liquid-material ratio of 10 mL/g, leaching for 2 days at room temperature, and filtering through sterile gauze to obtain the achyranthes bidentata chrysanthemum leaching liquor.
The achyranthes bidentata leaching liquor can be used for preventing and treating bacterial wilt of crops.
The invention has the advantages that:
the achyranthes bidentata chrysanthemum leaching liquor prepared by the invention has obvious inhibition effect on various crop bacterial wilt germs, has strong continuous action, has the advantages of no pollution, no residue, no toxicity, higher safety on environment and the like compared with chemical pesticides, and provides a new strategy for biologically preventing and treating crop bacterial wilt.
Drawings
FIG. 1 is a comparison of the bacteriostatic effects of cyathula officinalis leaching solutions of different concentrations in example 1 on Ralstonia solanacearum.
FIG. 2 is a comparison of the bacteriostatic effects of the cyathula officinalis leaching solution on ralstonia solanacearum obtained in different leaching times in example 2.
FIG. 3 is a graph showing the plate bacteriostasis results of the aqueous extract of Achyranthus chamaemellus (A) and the phosphate extract of Achyranthus chamaemellus (B) obtained in leaching 2d in example 2.
Detailed Description
In order to make the present invention more comprehensible, the technical solutions of the present invention are further described below with reference to specific embodiments, but the present invention is not limited thereto.
Preparation of phosphate buffer solution: 11.856 g of NaH 2 PO 4 ·2H 2 O and 44.392 g of Na 2 HPO 4 ·12H 2 Dissolving O in 1L of distilled water to obtain 0.2moL/mL phosphate buffer solution with the pH value of 7.0.
EXAMPLE 1 plate inhibition Effect of Echinacea purpurea leach solutions of different concentrations on Ralstonia solanacearum
Obtaining an achyranthes bidentata chrysanthemum leaching solution: collecting fresh plants of the achyranthes bidentata Blume, cutting the plants into 2-5cm segments, placing 200g of cut achyranthes bidentata Blume in a sterile container, adding a certain amount of water or phosphate buffer solution respectively to make the concentrations of the water or phosphate buffer solution be 0.02g/mL, 0.04g/mL, 0.06g/mL, 0.08g/mL, 0.10g/mL and 0.20g/mL, standing and leaching for 2d indoors, filtering with sterile gauze to remove residues in the leaching solution to obtain an achyranthes bidentata Blume water leaching solution and an achyranthes bidentata Blume phosphate leaching solution, and placing in a refrigerator at 4 ℃ for later use.
The cyathula root chrysanthemum extract has plate bacteriostasis to crop ralstonia solanacearum: selecting a ralstonia solanacearum strain to be tested in an NB culture medium by an inhibition zone method, carrying out constant-temperature oscillation (28 ℃,180 r/min) culture for 24h, uniformly mixing a bacterial suspension and a properly-heated and well-melted CPG solid culture medium according to a ratio of 1; respectively sucking achyranthes bidentata water extractive solution or achyranthes bidentata phosphate extractive solution (5 μ L) with different concentrations on a plate, air drying, culturing in 28 deg.C incubator, and repeating each treatment for 3 times.
As shown in the result of figure 1, in the range of 0.08-0.20 g/mL, the bacteriostatic effect of the achyranthes bidentata chrysanthemum phosphate leaching liquor is obviously better than that of the achyranthes bidentata chrysanthemum water leaching liquor, and the achyranthes bidentata chrysanthemum phosphate leaching liquor has the best bacteriostatic effect when the concentration is 0.10g/mL, and the average radius of the bacteriostatic zone is 4.33mm.
Example 2 bacteriostatic effect of cyathula officinalis leaching solution on bacterial wilt of tobacco for different leaching time
Obtaining an achyranthes bidentata chrysanthemum leaching solution: collecting fresh plants of Echinacea pallida, cutting into 2-5cm segments, placing 200g of cut Echinacea pallida in a sterile container, adding 1L of water or phosphate buffer solution, standing and leaching in a room for different times, filtering with sterile gauze to remove residue in the leaching solution to obtain an aqueous extract of Echinacea pallida and a phosphate leaching solution of Echinacea pallida, and placing in a refrigerator at 4 deg.C for use.
The cyathula root chrysanthemum extract has plate bacteriostasis to crop ralstonia solanacearum: selecting a bacterial strain of the ralstonia solanacearum to be tested in an NB culture medium by adopting an inhibition zone method, culturing for 24 hours under constant temperature oscillation (28 ℃,180 r/min), uniformly mixing a bacterial suspension and a CPG solid culture medium which is melted at a proper temperature according to the proportion of 1; respectively sucking the above extracts (5 μ L) on a plate, air drying, culturing in 28 deg.C incubator, and repeating each treatment for 3 times.
As shown in the results of FIG. 2, the bacteriostatic effect of the water extract of Achyranthus niveus obtained by leaching 2d and the phosphate extract of Achyranthus niveus were the best, and the radius of the zone of inhibition of the water extract and the phosphate extract of Achyranthus niveus were 3.33mm and 3.67mm, respectively (as shown in FIG. 3).
Example 3 bacteriostatic effect of Echinacea purpurea leaching solution on plate of crop pathogenic bacteria
Determining Achyranthis radix flos Chrysanthemi leaching solution (with concentration of 0.10g/mL, obtained by leaching for 2 d) for treating tobacco Ralstonia solanacearum by bacteriostatic ring methodRalstonia solanacearium) And tomato Ralstonia solanacearum (A. Solanacearum: (A. Solanacearum)R. solanacearium) And Ralstonia solanacearum (sweet potato)R. solanacearium) Bacterial wilt of potato (R. solanacearium) Ralstonia solanacearum (B) ((B))R. solanacearium) Tobacco cavity pathogen: (Pectobacterium carotovorum) Tobacco wild fire pathogen: (Pseudomonas amygdali) Bacterial spot of tomato (A), (B)P. syringae) Pseudomonas chicory (A)P. cichorii) Chinese cabbage Soft rot fungus (B)P. carotovorum) Bacteria of common scab of Capsicum (A), (B), (C)Xanthomonas euvesicatoria) And the results are shown in table 1.
TABLE 1 bacteriostatic effect of achyranthes bidentata leaching liquor on plate of crop pathogenic bacteria
The results in table 1 show that the achyranthes bidentata water extract and the achyranthes bidentata phosphate extract both have certain inhibition effects on different crop ralstonia solanacearum, but have no inhibition effect on plant pathogenic bacteria such as tobacco vacuity bacteria, tobacco wildfire bacteria, tomato bacterial spot bacteria, chicory pseudomonas, pakchoi soft rot bacteria, capsicum scabies and the like, which proves that the achyranthes bidentata water extract has specificity on the inhibition effect on ralstonia solanacearum, wherein the inhibition effect of the achyranthes bidentata phosphate extract is superior to that of the achyranthes bidentata water extract.
Example 4 prevention and treatment effects of Echinacea purpurea leaching solution on tobacco bacterial wilt
Selecting healthy tobacco seedlings with consistent growth vigor, respectively irrigating 30mL cyathula officinalis leaching solution with concentration of 0.10g/mL (6 plants are treated each, and each treatment is repeated for 3 times), irrigating roots after 24h, and inoculating 30mL OD 600 =0.8 suspension of ralstonia solanacearum, to examine the control effect of the cyathula extract on tobacco bacterial wilt (using zhongshengmycin as control), and the results are shown in table 2.
TABLE 2 prevention and treatment effects of cyathula officinalis extract on tobacco bacterial wilt
As can be seen from the results in Table 2, after inoculation, the control effects of the achyranthes bidentata water extract and the achyranthes bidentata phosphate extract on tobacco bacterial wilt are superior to those of zhongshengmycin, and the control effect of the achyranthes bidentata phosphate extract is superior to that of the achyranthes bidentata water extract.
The above description is only a preferred embodiment of the present invention, and all equivalent changes and modifications made in accordance with the claims of the present invention should be covered by the present invention.
Claims (3)
1. A preparation method of cyathula root chrysanthemum leaching liquor for preventing and treating crop bacterial wilt is characterized by comprising the following steps: cutting fresh achyranthes bidentata chrysanthemum into small sections of 2-5cm, adding phosphate buffer solution according to the liquid-material ratio of 10 mL/g, leaching at room temperature for 2d, and filtering through sterile gauze to obtain the achyranthes bidentata chrysanthemum leaching liquor.
2. The method for preparing the achyranthes bidentata leaching solution for controlling the bacterial wilt of crops according to claim 1, wherein the method comprises the following steps: the concentration of the phosphate buffer was 0.20moL/mL, pH =7.
3. Use of the cyathula officinalis leaching solution prepared according to the method of claim 1 for controlling bacterial wilt of crops.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103447290A (en) * | 2013-08-23 | 2013-12-18 | 林立金 | Remediation method for heavy metal cadmium pollution of orchard soil based on galinsoga parviflora |
| CN107628881A (en) * | 2017-10-31 | 2018-01-26 | 博白县富山水果种植专业合作社 | A kind of insect prevention base manure of lichee and preparation method thereof |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103447290A (en) * | 2013-08-23 | 2013-12-18 | 林立金 | Remediation method for heavy metal cadmium pollution of orchard soil based on galinsoga parviflora |
| CN107628881A (en) * | 2017-10-31 | 2018-01-26 | 博白县富山水果种植专业合作社 | A kind of insect prevention base manure of lichee and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 范雪涛: "入侵植物辣子草抗逆性及化感作用的初步研究", 《中国优秀硕士学位论文全文数据库 农业科技辑》, pages 4 * |
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