CN115363014A - Insect memory, insect memory device and memory method - Google Patents

Insect memory, insect memory device and memory method Download PDF

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Publication number
CN115363014A
CN115363014A CN202211158934.9A CN202211158934A CN115363014A CN 115363014 A CN115363014 A CN 115363014A CN 202211158934 A CN202211158934 A CN 202211158934A CN 115363014 A CN115363014 A CN 115363014A
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insect
storage device
insects
larvae
padding
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CN115363014B (en
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杨朝傑
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Xi'an Bulupting Biotechnology Co ltd
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Xi'an Bulupting Biotechnology Co ltd
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Priority to PCT/CN2022/142773 priority patent/WO2024060455A1/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00Preservation of bodies of humans or animals, or parts thereof
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/033Rearing or breeding invertebrates; New breeds of invertebrates

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Dentistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Catching Or Destruction (AREA)

Abstract

The application relates to an insect memory, an insect memory device and a memory method. In the environment of 10-28 ℃, the water evaporation speed of the padding mixed with the insects in the insect storage device is less than or equal to 0.5ml/h/100g. This application is packed into the memory after mixing insect and bedding and padding for the insect obtains the save of longer time.

Description

Insect memory, insect memory device and memory method
Technical Field
The present application relates to the field of insect preservation, and in particular, to an insect storage device, an insect storage apparatus, and a storage method.
Background
The insects are difficult to store and transport, for example, no storage technology exists for the low-day-old larvae of the diptera insects at present, and the transportation in the egg state of the diptera insects can be realized at present. However, in the transportation process of the diptera insect eggs, due to the fact that the eggs are extremely fragile, time consumption and environmental change in the storage and transportation process can cause death in a large proportion, the death rate of the eggs cannot be ensured, the fertilization rate and the effective hatching rate of the eggs cannot be ensured, and the survival rate of the larvae which are cultivated to be of low-day age after hatching cannot be ensured, so that the fixed amount of the eggs cannot be transported to the downstream breeding and fattening link, and the stable production of the large-weight larvae (mainly, the body length of the larvae is more than 20 mm) with fixed amount and capable of being commercialized cannot be ensured.
Disclosure of Invention
Based on the above problems, the present application provides an insect storage device, an insect storage apparatus, and a storage method, which are convenient for storing insects.
One embodiment of the application provides an insect storage device, and in an environment of 10-28 ℃, the water evaporation speed of a pad mixed with insects in the insect storage device is less than or equal to 0.5ml/h/100g.
According to some embodiments of the present application, the insect reservoirs may be composed of the same material or may be composed of different materials. The insect trap may be composed of the same material, preferably the insect trap is composed of a non-woven fabric having a grammage of 30-200g per square meter.
According to some embodiments of the application, the insect memory comprises: a closing part; the air permeable part is connected with the sealing part, an air permeable hole is formed in the air permeable part, the sealing part and the air permeable part jointly form an insect storage space, and the surface area of the air permeable part is 10% -70% of the total surface area of the insect storage device.
According to some embodiments of the application, the insect store comprises a storage bag or box.
According to some embodiments of the present application, the ventilation part includes a nonwoven fabric, and at least some of the ventilation holes are located on the nonwoven fabric.
According to some embodiments of the present application, the hole density of the airing holes is 1 to 5000 holes per square centimeter.
One embodiment of the present application provides an insect storage device comprising: an insect reservoir as described above; and the padding is arranged in the insect storage device and is used for bearing the insects.
According to some embodiments of the application, the weight ratio of litter to insects is 1.2-2.6.
According to some embodiments of the present application, the mat has a porosity of 40% to 90%, a water content of 20% to 55%, and an average particle size of 2 to 6mm.
According to some embodiments of the present application, the composition of the litter includes one or more of insect manure, compost matrix, coconut coir, straw, wood chips, wheat hulls, and rice hulls.
One embodiment of the present application provides a method of storing insects, comprising: mixing the insects with the bedding; loading the litter mixed with the insects into an insect reservoir; the insect storage device is placed in an environment with the temperature of 5-30 ℃, and the water evaporation speed of the padding mixed with the insects in the insect storage device is less than or equal to 0.5ml/h/100g.
The storage device is filled with the mixed insects and the padding, and in a proper environment, the structure of the storage device can control the moisture evaporation speed of the padding mixed with the insects, so that the insects can be stored for a long time.
Drawings
In order to more clearly illustrate the technical solutions of the present application, the drawings needed to be used in the description of the embodiments are briefly introduced below, it is obvious that the drawings in the following description are only some embodiments of the present application, and it is obvious for a person skilled in the art to obtain other drawings based on these drawings without exceeding the protection scope of the present application.
FIG. 1 is a schematic view of a storage bag according to an embodiment of the present application;
FIG. 2 is a schematic view of a first storage case of an embodiment of the present application;
FIG. 3 is a schematic view of a second storage case of an embodiment of the present application;
FIG. 4 is a schematic illustration of a dunnage according to an embodiment of the present application;
FIG. 5 is a schematic view of a transport basket according to an embodiment of the present application.
Detailed Description
The technical solutions of the present application will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present application, and it is obvious that the described embodiments are some, not all, of the embodiments of the present application. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.
For example, for hermetia illucens, the hermetia illucens can only be stored and transported by eggs, when the hermetia illucens are stored and transported in an egg state, a considerable proportion of hermetia illucens eggs can be hatched in the transportation process, the maximum survival time of the hermetia illucens and 1-day-old larvae (newly hatched larvae) in the storage and transportation environment cannot exceed 5 days, the death rate is very high within 3-5 days, and the requirements on transportation timeliness and industrial production timeliness entering the incubation and cultivation stage are very high. The embodiment provides a method for storing insects, which includes the steps of immediately providing hatching conditions for hermetia illucens eggs after collection, breeding the hermetia illucens eggs to low-day-age larvae, greatly improving the survival capacity of the larvae, and storing the hermetia illucens low-day-age larvae, so that the problem that the production efficiency of a downstream fattening link is affected due to the fact that the mortality, fertilized eggs and the hatchability cannot be guaranteed is solved. Meanwhile, a raw material allocation buffer period of industrial production is provided for the fattening link, and the industrial production allocation of the fattening link can be realized by purchasing larva raw materials for a long time at one time, so that the actual yield is improved, and the production efficiency is improved. The lower-day-old black soldier fly larvae are larvae with body length of 1-10 mm.
The embodiment provides an insect storage device, which is placed in an environment with the temperature of 10-28 ℃ to store insects, wherein the water evaporation speed of a pad mixed with the insects in the insect storage device is less than or equal to 0.5ml/h/100g. For example, the black soldier fly larvae of low day age are stored in the insect storage device, and can be dormant in an environment of 10-28 ℃, so that long-time storage of more than 30 days can be realized.
As shown in fig. 1, the insect storage device comprises a sealing part 1 and a ventilating part 2, wherein the ventilating part 2 is connected with the sealing part 1 to form a storage space for insects, for example, for storing low-day-old hermetia illucens larvae. The sealing part 1 is made of air-proof material, the air-permeable part 2 is provided with an air hole 21, and the air hole 21 allows air to circulate, so that the air exchange between the inside and the outside of the insect storage device is realized. The surface area of the air-permeable part 2 is 10-70% of the total surface area of the insect storage device. The ratio of the surface area of the air-permeable part 2 to the total surface area of the insect storage device is set as required.
For example, the insect storage device is a storage bag 100, a part of the front surface and the whole back surface of the storage bag 100 are plastic films, the plastic films are sealing parts 1, the other part of the front surface of the storage bag 100 is a non-woven fabric with the gram weight per square meter of 30-200g, the non-woven fabric has air permeability, and the non-woven fabric is an air permeable part 2. The interior space of the storage bag 100 is used for storing lower day-old hermetia illucens larvae. Optionally, the top of the storage bag 100 is provided with a plastic sealing line 101, and the plastic sealing line 101 is used for closing the top end opening of the storage bag 100. A tear-off opening 102 is provided below the plastic sealing line 101, and the storage bag 100 is easily opened through the tear-off opening 102.
Alternatively, the insect reservoir may be composed of the same material, for example, the insect reservoir is composed of a non-woven fabric having a grammage of 30-200g per square meter.
The insect memory of this embodiment has ventilative function of moisturizing, can save the low-age larva of heisui river horsefly, and in suitable environment, the moisture evaporation rate of material in the memory can be controlled to the structure of memory for the low-age larva of heisui river horsefly obtains the save of longer time.
As shown in fig. 2, in an alternative embodiment, the insect storage device is a first storage box 200, the first storage box 200 includes a first box body 201 and a first cover 202, and the first cover 202 is disposed on the top of the first box body 201. The first storage case 200 has an overall cylindrical shape. A circular hole is formed in the top of the first cover 202, and the circular hole in the top of the first cover 202 is covered by the ventilation part 2. Alternatively, the air-permeable portion 2 is a nonwoven fabric having a grammage of 30 to 200g per square meter through which air passes. The remaining portions of the first container 201 and the first cover 202 are the closing portions 1.
As shown in figure 3, in an alternative embodiment, the insect storage device is a second storage box 300, and the second storage box 300 comprises a second box body 301 and a second cover 302, and the second cover 302 is disposed on the top of the second box body 301. The second storage case 300 is a rectangular parallelepiped as a whole. The side wall of the second box body 301 is provided with a vent hole 21, the part of the second box body 301 provided with the vent hole 21 is arranged at the vent part 2, and the rest part of the second box body 301 and the second box cover 302 are the closed part 1.
Alternatively, the air-permeable portion 2 includes a nonwoven fabric, for example, a nonwoven fabric having a grammage of 30 to 200g per square meter. At least part of the air holes 21 are holes on the non-woven fabric. Other air permeable materials may be used for the air permeable portion 2, for example, the air permeable portion 2 is a waterproof air permeable molecular membrane, such as an EPTFE molecular membrane.
In an alternative, the hole density of the ventilation holes 21 in the ventilation portion 2 is 1 to 5000 holes per square centimeter, for example, 5 holes per square centimeter, 10 holes per square centimeter, 100 holes per square centimeter, 1000 holes per square centimeter. Optionally, the aperture of the air holes 21 is less than 2mm, so that the materials in the insect storage device are prevented from falling out of the air holes 21.
As shown in fig. 4, this embodiment also provides an insect storage device comprising an insect storage as described above and a litter 400, the litter 400 being arranged in the insect storage, the litter being for carrying insects, such as hermetia illucens low-day-age larvae. In use, the black soldier fly low day age larvae are mixed with the litter 400 in a predetermined ratio and the litter of mixed larvae is then loaded into an insect storage container, such as a storage bag or box as described above. At a proper temperature, the lower-day-old hermetia illucens larvae in the insect storage device enter a dormant period so as to realize long-time storage of the larvae.
In an alternative, when the litter 400 is mixed with the insects, the weight ratio of the litter 400 to the insects is 1.2 to 2.6. Excessive padding 400 causes the waste of padding, and too little padding 400 is difficult to meet the requirements of low-day-old larvae of hermetia illucens.
Optionally, the porosity of the mat 400 is 40% to 90% to ensure the air permeability of the mat 400. Porosity refers to the percentage of the pore volume in the particulate material to the total volume of the material in its natural state. The water content of the padding 400 is 20% -55%, the water content required for the low-day-age larvae of the hermetia illucens to live is met, and the water content of the padding 400 is too low to meet the survival requirement of the low-day-age larvae of the hermetia illucens. The moisture content is a ratio of the weight of moisture contained in the mat 400 to the total weight of the mat 400. The average particle size of the mat 400 is 2 to 6mm, for example, the average particle size of the mat 400 is 3.5mm. If the grain size of the padding is less than 2mm, the internal air permeability of the whole padding is insufficient or the padding is attached to the body surface of larvae, and part of the larvae cannot obtain air for breathing and survival, so that oxygen deficiency and death are caused; if the grain size of the padding is higher than 6mm, the water in the padding can be quickly lost, and part of larvae die due to water shortage. In an alternative embodiment, the litter 400 may comprise one or more of insect manure, compost matrix, coconut coir, straw, wood chips, wheat hulls, and rice hulls. For example, the dung of the black soldier fly larvae is the dung of the black soldier fly larvae. The present embodiment is not limited to the weight percentage of the composition of the dunnage 400.
By controlling the components, porosity, water content, average particle size and weight ratio of the padding 400 to the insects, the padding mixed with the insects is guaranteed to be soft and breathable as a whole and wet but not show that clear water seeps out.
As shown in fig. 5, the present example provides a transport basket 500, the transport basket 500 being open at the top end thereof, and a plurality of insect storages are arranged in one transport basket 500 during transportation, thereby facilitating handling during mass transportation. Optionally, ventilation holes 510 are provided on the side walls of the transport basket 500.
The embodiment provides a method for storing insects, which comprises the following steps:
1. mixing insects and padding according to a preset proportion;
2. loading a litter mixed with insects into an insect reservoir;
3. the insect storage device is placed in the environment with the temperature of 10-28 ℃ to store the insects, and the water evaporation speed of the padding mixed with the insects in the insect storage device is less than or equal to 0.5ml/h/100g. Optionally, the insect is a black soldier fly low day old larva.
By storing the low-day-age larvae of the hermetia illucens through the method, the low-day-age larvae of the hermetia illucens can reach a dormant state, and long-time storage of more than 30 days is realized.
Example 1
The insect storage is made of non-woven fabric with the gram weight of 200g per square meter.
The method comprises the following steps of mixing the low-day-old hermetia illucens larvae with padding, wherein the padding is a mixture of hermetia illucens larva manure, culture soil matrix, coconut chaff, straw, wood dust, wheat hulls and rice hulls, the weight ratio of the padding to the low-day-old hermetia illucens larvae is 1.2. The litter mixed with larvae is loaded into the insect reservoir. The survival rate of the lower day-old black soldier fly larvae when the insect reservoir is placed in an environment at 10 ℃ is shown in table 1.
Example 2
The insect storage device is a storage bag, the storage bag comprises a closed part and a ventilating part, the closed part is a plastic film, the ventilating part is a non-woven fabric with the gram weight per square meter of 100g, and the surface area of the ventilating part is 30% of the total surface area of the storage bag.
The method comprises the following steps of mixing the low-day-old black soldier fly larvae with padding, wherein the padding comprises a mixture of culture soil matrix, coconut coir, straw, wood chips, wheat shells and rice hulls, the weight ratio of the padding to the low-day-old black soldier fly larvae is 1.8, the porosity of the padding is 55%, the water content of the padding is 40%, and the average particle size of the padding is 3.5mm. The litter mixed with larvae is loaded into an insect reservoir. The survival rate of the low-day-old black soldier fly larvae is shown in table 1 when the insect storage device is placed in an environment at the temperature of 16 ℃.
Example 3
Insect memory is the storage box, is provided with per square meter grammes per square meter for 80g non-woven fabrics on the top cap of storage box, and the rest of storage box is airtight PVC material, and the surface area of non-woven fabrics is 20% of the total surface area of storage box.
The method comprises the following steps of mixing the low-day-old hermetia illucens larvae with padding, wherein the padding comprises a mixture of culture soil matrix, coconut coir, straw, wood chips, wheat shells and rice hulls, the weight ratio of the padding to the low-day-old hermetia illucens larvae is 2.6, the porosity of the padding is 90%, the water content of the padding is 55%, and the average particle size of the padding is 6mm. The litter mixed with larvae is loaded into the insect reservoir. The survival rate of lower day-old black soldier fly larvae when the insect reservoir is placed in an environment at 28 ℃ is shown in table 1.
Comparative example 1
The lower day-old larvae of hermetia illucens mixed with the bedding in example 2 were stored in a conventional storage container for eggs of hermetia illucens, and the conventional storage container was placed in an environment of 20 ℃, wherein the water evaporation rate of the bedding in which the larvae were mixed in the conventional storage container was 1ml/h/100g, and the survival rate of the lower day-old larvae of hermetia illucens was shown in table 1.
Comparative example 2
The insect storage device is a storage bag, the storage bag comprises a closed part and a ventilating part, the closed part is a plastic film, the ventilating part is a non-woven fabric with the gram weight per square meter of 100g, and the surface area of the ventilating part is 30% of the total surface area of the storage bag.
The method comprises the following steps of mixing the low-day-old hermetia illucens larvae with padding, wherein the padding comprises a mixture of culture soil matrix, coconut coir, straw, wood chips, wheat shells and rice hulls, the weight ratio of the padding to the low-day-old hermetia illucens larvae is 1.8, the porosity of the padding is 20%, the water content of the padding is 40%, and the average particle size of the padding is 1mm. The litter mixed with larvae is loaded into the insect reservoir. The survival rate of the low-day-old black soldier fly larvae is shown in table 1 when the insect storage device is placed in an environment at the temperature of 16 ℃.
Comparative example 3
The insect storage device is a storage bag, the storage bag comprises a closed part and a ventilating part, the closed part is a plastic film, the ventilating part is a non-woven fabric with the gram weight per square meter of 100g, and the surface area of the ventilating part is 30% of the total surface area of the storage bag.
The method comprises the following steps of mixing the low-day-old hermetia illucens larvae with padding, wherein the padding comprises a mixture of culture soil matrix, coconut coir, straw, wood chips, wheat shells and rice hulls, the weight ratio of the padding to the low-day-old hermetia illucens larvae is 1.8, the porosity of the padding is 95%, the water content of the padding is 40%, and the average particle size of the padding is 8mm. The litter mixed with larvae is loaded into the insect reservoir. The survival rate of lower day-old black soldier fly larvae when the insect reservoir was placed in an environment at 16 ℃ is shown in table 1.
The method for counting the survival rate comprises the following steps:
(1) Counting the initial number of larvae thrown in the process of mixing with padding;
(2) After the preset number of days is stored, separating larvae from padding in an insect storage device, weighing the total weight of the larvae, counting the number of the larvae of 100g larvae, and determining the total number of the larvae after the preset number of days is stored according to the number of the larvae of 100g larvae and the total weight of the larvae;
(3) The survival rate is the ratio of the total number of larvae after storing the preset number of days to the initial number of larvae.
Survival rate of low-day-old black soldier fly larvae
7 days 15 days 30 days
Example 1 98% 94.5% 90.5%
Example 2 99% 96% 95%
Example 3 98% 95.5% 92%
Comparative example 1 72% 31% 0%
Comparative example 2 60% 17% 0%
Comparative example 3 85% 35% 0%
The embodiments of the present application are described in detail above. The principle and the implementation of the present application are explained herein by applying specific examples, and the above description of the embodiments is only used to help understand the technical solutions and the core ideas of the present application. Therefore, the person skilled in the art should, according to the idea of the present application, change or modify the embodiments and applications of the present application based on the scope of protection of the present application. In view of the above, the description should not be taken as limiting the application.

Claims (10)

1. An insect storage device is characterized in that in an environment of 10-28 ℃, the moisture evaporation speed of a pad material mixed with insects in the insect storage device is less than or equal to 0.5ml/h/100g.
2. An insect memory according to claim 1, comprising:
a closing part;
the air permeable part is connected with the closed part and provided with air holes, the closed part and the air permeable part jointly form an insect storage space, and the surface area of the air permeable part is 10% -70% of the total surface area of the insect storage device.
3. Insect store according to claim 2, characterized in that the insect store comprises a storage bag or a storage box.
4. The insect memory according to claim 2, wherein the ventilation part comprises a non-woven fabric, and at least a portion of the ventilation holes are formed in the non-woven fabric.
5. The insect memory according to claim 2, wherein the hole density of the ventilation holes is 1 to 5000 holes per square centimeter.
6. An insect storage device, comprising:
an insect store according to any one of claims 1 to 5;
a litter disposed in the insect reservoir, the litter for carrying insects.
7. The insect storage device of claim 6 wherein the weight ratio of litter to insects is 1.2-2.6.
8. An insect storage device as claimed in claim 7, wherein the mat has a porosity of 40-90%, a moisture content of 20-55%, and an average particle size of 2-6 mm.
9. The insect storage device of claim 6 wherein the composition of the litter includes one or more of insect feces, compost matrix, coconut chaff, straw, wood chips, wheat hulls, and rice hulls.
10. A method of storing insects, comprising:
mixing the insects with the bedding;
loading a litter mixed with insects into an insect reservoir;
the insect storage device is placed in an environment with the temperature of 10-28 ℃, and the water evaporation speed of the padding mixed with the insects in the insect storage device is less than or equal to 0.5ml/h/100g.
CN202211158934.9A 2022-09-22 2022-09-22 Insect memory, insect storage device and storage method Active CN115363014B (en)

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WO2024060455A1 (en) * 2022-09-22 2024-03-28 西安布鲁普庭生物科技有限公司 Insect storage device, and insect storage apparatus and method
CN116267807A (en) * 2023-02-14 2023-06-23 西安布鲁普庭生物科技有限公司 Larva separator and larva separation method

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