CN115335704A - 用于检测腺相关病毒的方法和试剂盒 - Google Patents

用于检测腺相关病毒的方法和试剂盒 Download PDF

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Publication number
CN115335704A
CN115335704A CN202180023044.4A CN202180023044A CN115335704A CN 115335704 A CN115335704 A CN 115335704A CN 202180023044 A CN202180023044 A CN 202180023044A CN 115335704 A CN115335704 A CN 115335704A
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CN
China
Prior art keywords
aav
binds
kit
reagent
capture reagent
Prior art date
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Pending
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CN202180023044.4A
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English (en)
Chinese (zh)
Inventor
王鹏
顾炳南
A·赛思
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Lonza Houston Inc
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Lonza Houston Inc
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Application filed by Lonza Houston Inc filed Critical Lonza Houston Inc
Publication of CN115335704A publication Critical patent/CN115335704A/zh
Pending legal-status Critical Current

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56983—Viruses
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/62—Detectors specially adapted therefor
    • G01N30/72—Mass spectrometers
    • G01N30/7233—Mass spectrometers interfaced to liquid or supercritical fluid chromatograph
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
    • G01N2333/01—DNA viruses
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
    • G01N2333/01—DNA viruses
    • G01N2333/075—Adenoviridae
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2469/00—Immunoassays for the detection of microorganisms
    • G01N2469/10—Detection of antigens from microorganism in sample from host

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Virology (AREA)
  • Physics & Mathematics (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CN202180023044.4A 2020-05-12 2021-05-10 用于检测腺相关病毒的方法和试剂盒 Pending CN115335704A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202063023629P 2020-05-12 2020-05-12
US63/023,629 2020-05-12
PCT/US2021/031577 WO2021231296A1 (en) 2020-05-12 2021-05-10 Methods and kits for detecting adeno-associated viruses

Publications (1)

Publication Number Publication Date
CN115335704A true CN115335704A (zh) 2022-11-11

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CN202180023044.4A Pending CN115335704A (zh) 2020-05-12 2021-05-10 用于检测腺相关病毒的方法和试剂盒

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Country Link
US (1) US20230168250A1 (https=)
EP (2) EP4143577B1 (https=)
JP (1) JP7763771B2 (https=)
KR (1) KR20230009377A (https=)
CN (1) CN115335704A (https=)
CA (1) CA3172980A1 (https=)
IL (1) IL298134A (https=)
WO (1) WO2021231296A1 (https=)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4363597A1 (en) * 2022-01-20 2024-05-08 Sartorius Xell GmbH Method for the detection and quantification of adeno-associated viruses (aavs) using an affinity matrix
CN119452254A (zh) * 2022-04-18 2025-02-14 万迈医疗仪器有限公司 用于确定dna病毒中的dna浓度的方法
EP4705768A1 (en) * 2023-05-05 2026-03-11 Regeneron Pharmaceuticals, Inc. Labeling and anti-drug antibody assays for aav vectors

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019067982A2 (en) * 2017-09-29 2019-04-04 Massachusetts Eye And Ear Infirmary PRODUCTION OF ADENO-ASSOCIATED VIRUSES IN INSECT CELLS

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AU4954796A (en) * 1995-03-17 1996-10-08 Hisamitsu Pharmaceutical Co., Inc. Monoclonal antibody specifically recognizing adeno-associated virus cap protein
US7419817B2 (en) * 2002-05-17 2008-09-02 The United States Of America As Represented By The Secretary Department Of Health And Human Services, Nih. Scalable purification of AAV2, AAV4 or AAV5 using ion-exchange chromatography
EP1724359A1 (en) * 2005-05-18 2006-11-22 PerkinElmer Life and Analytical Sciences B.V. Luciferase assay system
SG169395A1 (en) * 2006-02-09 2011-03-30 Univ South Florida Detection of cancer by elevated levels of bcl-2
WO2016035197A1 (ja) * 2014-09-05 2016-03-10 株式会社日立製作所 電気化学免疫センサ用カートリッジ及びそれを用いた測定装置
CN108603235A (zh) * 2015-07-30 2018-09-28 马萨诸塞眼科耳科诊所 祖先病毒序列及其用途
JP7467119B2 (ja) 2017-02-17 2024-04-15 ロンザ リミテッド 発現困難タンパク質のための多部位ｓｓｉ細胞
WO2018170015A1 (en) * 2017-03-14 2018-09-20 The Regents Of The University Of California Engineering crispr cas9 immune stealth
WO2019212922A1 (en) * 2018-04-29 2019-11-07 Regenxbio Inc. Systems and methods of spectrophotometry for the determination of genome content, capsid content and full/empty ratios of adeno-associated virus particles
WO2021067874A1 (en) * 2019-10-02 2021-04-08 Oregon Health & Science University Production of anti-viral monoclonal antibodies by hydrodynamic-based transfection

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019067982A2 (en) * 2017-09-29 2019-04-04 Massachusetts Eye And Ear Infirmary PRODUCTION OF ADENO-ASSOCIATED VIRUSES IN INSECT CELLS

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
BRITTNEY L. GURDA等: "Mapping a Neutralizing Epitope onto the Capsid of Adeno-Associated Virus Serotype 8", JOURNAL OF VIROLOGY, vol. 86, no. 15, 16 May 2012 (2012-05-16), pages 7739 - 7751, XP055590574, DOI: 10.1128/JVI.00218-12 *

Also Published As

Publication number Publication date
CA3172980A1 (en) 2021-11-18
EP4143577B1 (en) 2025-12-10
EP4692800A3 (en) 2026-04-22
JP2023525204A (ja) 2023-06-15
EP4692800A2 (en) 2026-02-11
US20230168250A1 (en) 2023-06-01
KR20230009377A (ko) 2023-01-17
IL298134A (en) 2023-01-01
JP7763771B2 (ja) 2025-11-04
WO2021231296A1 (en) 2021-11-18
EP4143577A1 (en) 2023-03-08

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