CN115298306A - 转谷氨酰胺酶变体 - Google Patents

转谷氨酰胺酶变体 Download PDF

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Publication number
CN115298306A
CN115298306A CN202180019827.5A CN202180019827A CN115298306A CN 115298306 A CN115298306 A CN 115298306A CN 202180019827 A CN202180019827 A CN 202180019827A CN 115298306 A CN115298306 A CN 115298306A
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CN
China
Prior art keywords
product
transglutaminase
protein
enzyme
sequence
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Pending
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CN202180019827.5A
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English (en)
Chinese (zh)
Inventor
W.辛德尔
E.米尔切克
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Curie Co Inc
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Curie Co Inc
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Publication of CN115298306A publication Critical patent/CN115298306A/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/104—Aminoacyltransferases (2.3.2)
    • C12N9/1044—Protein-glutamine gamma-glutamyltransferase (2.3.2.13), i.e. transglutaminase or factor XIII
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/50—Isolated enzymes; Isolated proteins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/43—Enzymes; Proenzymes; Derivatives thereof
    • A61K38/45—Transferases (2)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
    • A61K8/66—Enzymes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A61P17/02—Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
    • A61Q17/04—Topical preparations for affording protection against sunlight or other radiation; Topical sun tanning preparations
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2795/00—Bacteriophages
    • C12N2795/00011—Details
    • C12N2795/00041—Use of virus, viral particle or viral elements as a vector
    • C12N2795/00043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/02—Aminoacyltransferases (2.3.2)
    • C12Y203/02013—Protein-glutamine gamma-glutamyltransferase (2.3.2.13), i.e. transglutaminase or factor XIII
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C40B40/08—Libraries containing RNA or DNA which encodes proteins, e.g. gene libraries

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Epidemiology (AREA)
  • Dermatology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Pest Control & Pesticides (AREA)
  • Agronomy & Crop Science (AREA)
  • Dentistry (AREA)
  • Birds (AREA)
  • Environmental Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN202180019827.5A 2020-03-13 2021-03-10 转谷氨酰胺酶变体 Pending CN115298306A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202062988918P 2020-03-13 2020-03-13
US62/988,918 2020-03-13
PCT/US2021/021766 WO2021183680A1 (en) 2020-03-13 2021-03-10 Transglutaminase variants

Publications (1)

Publication Number Publication Date
CN115298306A true CN115298306A (zh) 2022-11-04

Family

ID=77671938

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202180019827.5A Pending CN115298306A (zh) 2020-03-13 2021-03-10 转谷氨酰胺酶变体

Country Status (9)

Country Link
US (1) US20230167420A1 (https=)
EP (1) EP4118194A4 (https=)
JP (1) JP2023518714A (https=)
KR (1) KR20220153047A (https=)
CN (1) CN115298306A (https=)
BR (1) BR112022017192A2 (https=)
CA (1) CA3172844A1 (https=)
MX (1) MX2022011077A (https=)
WO (1) WO2021183680A1 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117643311A (zh) * 2023-12-01 2024-03-05 北京舜雷科技有限公司 一种组合物、制备方法及应用

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20250304927A1 (en) 2022-05-20 2025-10-02 Curie Co. Inc. Pro-peptide variants for modulating activity of transglutaminases
CN115487345B (zh) * 2022-08-22 2024-03-12 天津大学 基于丝素蛋白的止血水凝胶和止血海绵及其制备方法和应用
KR20260013477A (ko) 2023-05-19 2026-01-28 큐리 컴퍼니 인코포레이티드 트랜스글루타미나제 및 그의 용도
CN121610553B (zh) * 2026-01-30 2026-04-17 四川友联味业食品有限公司 一种鲜味肽提取物复配取代味精的加工工艺

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1377413A (zh) * 1999-09-30 2002-10-30 味之素株式会社 转谷氨酰胺酶的制备方法
WO2008099898A1 (ja) * 2007-02-15 2008-08-21 Ajinomoto Co., Inc. ジスルフィド結合導入トランスグルタミナーゼ
US20090117640A1 (en) * 2005-08-18 2009-05-07 Novo Nordisk Health Care Ag Transglutaminase Variants with Improved Specificity
WO2010101256A1 (ja) * 2009-03-06 2010-09-10 味の素株式会社 放線菌由来の耐熱性トランスグルタミナーゼ
CN101945995A (zh) * 2008-02-13 2011-01-12 天野酶株式会社 稳定型转谷氨酰胺酶及其制造法
CN107739734A (zh) * 2017-10-26 2018-02-27 江南大学 一种酶活提高的谷氨酰胺转氨酶突变体
WO2019014118A1 (en) * 2017-07-09 2019-01-17 Igc Bio, Inc. PROSS OPTIMIZED ENZYMES

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ES2090001T3 (es) * 1994-02-14 1998-08-01 Univ Leiden Un metodo para seleccionar por la presencia de un defecto genetico asociado con trombosis y/o una pobre respuesta anticoagulante a la proteina c activada.
KR100575394B1 (ko) * 2000-08-17 2006-05-03 아지노모토 가부시키가이샤 미생물 유래 트랜스글루타미나제의 변이 방법
JP2008194004A (ja) * 2007-02-15 2008-08-28 Ajinomoto Co Inc 変異体トランスグルタミナーゼ
EP3706780A4 (en) * 2017-11-07 2021-12-22 Codexis, Inc. TRANSGLUTAMINASE VARIANTS
BR112020009793A2 (pt) * 2017-11-30 2020-11-03 Amano Enzyme Inc. transglutaminase modificada
CN113473856B (zh) * 2019-03-06 2024-03-19 居里公司 防腐剂组合物及其使用方法
MX2023002744A (es) * 2020-09-08 2023-04-03 Curie Co Inc Composiciones y metodos de uso de las mismas.

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1377413A (zh) * 1999-09-30 2002-10-30 味之素株式会社 转谷氨酰胺酶的制备方法
US20090117640A1 (en) * 2005-08-18 2009-05-07 Novo Nordisk Health Care Ag Transglutaminase Variants with Improved Specificity
WO2008099898A1 (ja) * 2007-02-15 2008-08-21 Ajinomoto Co., Inc. ジスルフィド結合導入トランスグルタミナーゼ
CN101631860A (zh) * 2007-02-15 2010-01-20 味之素株式会社 引入二硫键的转谷氨酰胺酶
CN101945995A (zh) * 2008-02-13 2011-01-12 天野酶株式会社 稳定型转谷氨酰胺酶及其制造法
WO2010101256A1 (ja) * 2009-03-06 2010-09-10 味の素株式会社 放線菌由来の耐熱性トランスグルタミナーゼ
CN102341497A (zh) * 2009-03-06 2012-02-01 味之素株式会社 放线菌来源的耐热性转谷氨酰胺酶
WO2019014118A1 (en) * 2017-07-09 2019-01-17 Igc Bio, Inc. PROSS OPTIMIZED ENZYMES
CN107739734A (zh) * 2017-10-26 2018-02-27 江南大学 一种酶活提高的谷氨酰胺转氨酶突变体

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CHRISTIAN K. MARX等: "Random mutagenesis of a recombinant microbial transglutaminase for the generation of thermostable and heat-sensitive variants", JOURNAL OF BIOTECHNOLOGY, vol. 136, no. 3, 10 September 2008 (2008-09-10), pages 156 - 162, XP024338589, DOI: 10.1016/j.jbiotec.2008.06.005 *
NCBI: "protein-glutamine gamma-glutamyltransferase [Streptomyces mobaraensis]", GENBANK DATABASE, 24 January 2015 (2015-01-24), pages 040890501 *
NORBERT E. JUETTNER等: "Structure of a glutamine donor mimicking inhibitory peptide shaped by the catalytic cleft of microbial transglutaminase", FEBS JOURNAL, vol. 285, no. 24, 15 October 2018 (2018-10-15), pages 4684 - 4694 *
任蕊蕊等: "分子改造提高谷氨酰胺转氨酶的催化活性", 食品与发酵工业, vol. 44, no. 9, 24 May 2018 (2018-05-24), pages 9 - 14 *
侯孝仑等: "原生质体融合提高产谷氨酰胺转氨酶菌株产量", 食品科学, vol. 37, no. 3, 17 July 2015 (2015-07-17), pages 145 - 150 *
王坤等: "转谷氨酰胺酶酶原在大肠杆菌中的重组优化表达", 食品工业科技, vol. 33, no. 7, 29 August 2011 (2011-08-29), pages 181 - 183 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117643311A (zh) * 2023-12-01 2024-03-05 北京舜雷科技有限公司 一种组合物、制备方法及应用
CN117643311B (zh) * 2023-12-01 2024-09-27 北京舜雷科技有限公司 一种组合物、制备方法及应用

Also Published As

Publication number Publication date
EP4118194A4 (en) 2024-08-21
MX2022011077A (es) 2022-09-23
US20230167420A1 (en) 2023-06-01
JP2023518714A (ja) 2023-05-08
EP4118194A1 (en) 2023-01-18
KR20220153047A (ko) 2022-11-17
BR112022017192A2 (pt) 2022-10-18
WO2021183680A1 (en) 2021-09-16
CA3172844A1 (en) 2021-09-16

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Application publication date: 20221104