CN115259941B - Small-molecular humic acid fertilizer and preparation method thereof - Google Patents
Small-molecular humic acid fertilizer and preparation method thereof Download PDFInfo
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- CN115259941B CN115259941B CN202210882244.1A CN202210882244A CN115259941B CN 115259941 B CN115259941 B CN 115259941B CN 202210882244 A CN202210882244 A CN 202210882244A CN 115259941 B CN115259941 B CN 115259941B
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- arthrobacter
- humic acid
- bacillus
- streptomyces
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- 239000003337 fertilizer Substances 0.000 title claims abstract description 92
- QJZYHAIUNVAGQP-UHFFFAOYSA-N 3-nitrobicyclo[2.2.1]hept-5-ene-2,3-dicarboxylic acid Chemical compound C1C2C=CC1C(C(=O)O)C2(C(O)=O)[N+]([O-])=O QJZYHAIUNVAGQP-UHFFFAOYSA-N 0.000 title claims abstract description 80
- 239000004021 humic acid Substances 0.000 title claims abstract description 80
- 238000002360 preparation method Methods 0.000 title claims abstract description 24
- 241000187747 Streptomyces Species 0.000 claims abstract description 53
- 241000186063 Arthrobacter Species 0.000 claims abstract description 43
- 241000228143 Penicillium Species 0.000 claims abstract description 37
- 239000003077 lignite Substances 0.000 claims abstract description 36
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 23
- 238000002156 mixing Methods 0.000 claims abstract description 21
- 241000193830 Bacillus <bacterium> Species 0.000 claims abstract description 16
- 238000012258 culturing Methods 0.000 claims abstract description 15
- 239000002994 raw material Substances 0.000 claims abstract description 11
- 244000005700 microbiome Species 0.000 claims abstract description 9
- 239000001963 growth medium Substances 0.000 claims abstract description 7
- 241001655322 Streptomycetales Species 0.000 claims abstract 2
- 238000000034 method Methods 0.000 claims description 32
- 150000003384 small molecules Chemical class 0.000 claims description 19
- 241000193749 Bacillus coagulans Species 0.000 claims description 15
- 241000187395 Streptomyces microflavus Species 0.000 claims description 15
- 229940054340 bacillus coagulans Drugs 0.000 claims description 15
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 claims description 11
- BWHMMNNQKKPAPP-UHFFFAOYSA-L potassium carbonate Substances [K+].[K+].[O-]C([O-])=O BWHMMNNQKKPAPP-UHFFFAOYSA-L 0.000 claims description 7
- 241000881860 Paenibacillus mucilaginosus Species 0.000 claims description 6
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 6
- 239000007633 bacillus mucilaginosus Substances 0.000 claims description 6
- 239000001301 oxygen Substances 0.000 claims description 6
- 229910052760 oxygen Inorganic materials 0.000 claims description 6
- 239000011573 trace mineral Substances 0.000 claims description 6
- 235000013619 trace mineral Nutrition 0.000 claims description 6
- 239000000618 nitrogen fertilizer Substances 0.000 claims description 5
- 239000002686 phosphate fertilizer Substances 0.000 claims description 5
- 229940072033 potash Drugs 0.000 claims description 5
- 235000015320 potassium carbonate Nutrition 0.000 claims description 5
- 241000194108 Bacillus licheniformis Species 0.000 claims description 3
- 244000063299 Bacillus subtilis Species 0.000 claims description 3
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 3
- 241000228153 Penicillium citrinum Species 0.000 claims description 3
- 241000187759 Streptomyces albus Species 0.000 claims description 3
- 241000187392 Streptomyces griseus Species 0.000 claims description 3
- 241000194105 Paenibacillus polymyxa Species 0.000 claims description 2
- 239000000463 material Substances 0.000 claims description 2
- 241000588986 Alcaligenes Species 0.000 claims 1
- 230000012010 growth Effects 0.000 abstract description 11
- 235000015097 nutrients Nutrition 0.000 abstract description 8
- 239000005556 hormone Substances 0.000 abstract description 7
- 229940088597 hormone Drugs 0.000 abstract description 7
- 230000000052 comparative effect Effects 0.000 description 18
- 241000196324 Embryophyta Species 0.000 description 16
- 230000000694 effects Effects 0.000 description 14
- 239000000126 substance Substances 0.000 description 10
- 239000000843 powder Substances 0.000 description 9
- 241000220225 Malus Species 0.000 description 7
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 6
- 235000019484 Rapeseed oil Nutrition 0.000 description 6
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 6
- 229930006000 Sucrose Natural products 0.000 description 6
- 239000011591 potassium Substances 0.000 description 6
- 229960003975 potassium Drugs 0.000 description 6
- 229910052700 potassium Inorganic materials 0.000 description 6
- 239000005720 sucrose Substances 0.000 description 6
- 238000012360 testing method Methods 0.000 description 6
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 5
- 239000011574 phosphorus Substances 0.000 description 5
- 229910052698 phosphorus Inorganic materials 0.000 description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- 244000046052 Phaseolus vulgaris Species 0.000 description 4
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 241000223600 Alternaria Species 0.000 description 3
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 3
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 3
- 241001661641 Verrucosa Species 0.000 description 3
- 239000002253 acid Substances 0.000 description 3
- 230000009471 action Effects 0.000 description 3
- 239000007864 aqueous solution Substances 0.000 description 3
- 230000004071 biological effect Effects 0.000 description 3
- 239000004202 carbamide Substances 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 230000003647 oxidation Effects 0.000 description 3
- 238000007254 oxidation reaction Methods 0.000 description 3
- 230000009467 reduction Effects 0.000 description 3
- 239000011734 sodium Substances 0.000 description 3
- 229910052708 sodium Inorganic materials 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- 235000019764 Soybean Meal Nutrition 0.000 description 2
- 230000003213 activating effect Effects 0.000 description 2
- 230000003197 catalytic effect Effects 0.000 description 2
- 239000011248 coating agent Substances 0.000 description 2
- 239000008139 complexing agent Substances 0.000 description 2
- 238000004090 dissolution Methods 0.000 description 2
- 230000001804 emulsifying effect Effects 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 150000007524 organic acids Chemical class 0.000 description 2
- 239000003895 organic fertilizer Substances 0.000 description 2
- 230000008635 plant growth Effects 0.000 description 2
- 230000001737 promoting effect Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 239000004455 soybean meal Substances 0.000 description 2
- 208000024891 symptom Diseases 0.000 description 2
- 230000002195 synergetic effect Effects 0.000 description 2
- CJUUXVFWKYRHAR-UHFFFAOYSA-M 1-Naphthaleneacetic acid sodium salt Chemical compound [Na+].C1=CC=C2C(CC(=O)[O-])=CC=CC2=C1 CJUUXVFWKYRHAR-UHFFFAOYSA-M 0.000 description 1
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 description 1
- 239000004114 Ammonium polyphosphate Substances 0.000 description 1
- 241000203069 Archaea Species 0.000 description 1
- ZOXJGFHDIHLPTG-UHFFFAOYSA-N Boron Chemical compound [B] ZOXJGFHDIHLPTG-UHFFFAOYSA-N 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- PWHULOQIROXLJO-UHFFFAOYSA-N Manganese Chemical compound [Mn] PWHULOQIROXLJO-UHFFFAOYSA-N 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- ZOKXTWBITQBERF-UHFFFAOYSA-N Molybdenum Chemical compound [Mo] ZOKXTWBITQBERF-UHFFFAOYSA-N 0.000 description 1
- 241000235395 Mucor Species 0.000 description 1
- GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 description 1
- 208000005374 Poisoning Diseases 0.000 description 1
- 241001136275 Sphingobacterium Species 0.000 description 1
- 208000000260 Warts Diseases 0.000 description 1
- 229910021536 Zeolite Inorganic materials 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 238000003916 acid precipitation Methods 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- LFVGISIMTYGQHF-UHFFFAOYSA-N ammonium dihydrogen phosphate Chemical compound [NH4+].OP(O)([O-])=O LFVGISIMTYGQHF-UHFFFAOYSA-N 0.000 description 1
- 229910000387 ammonium dihydrogen phosphate Inorganic materials 0.000 description 1
- 235000011114 ammonium hydroxide Nutrition 0.000 description 1
- -1 ammonium ions Chemical class 0.000 description 1
- 235000019826 ammonium polyphosphate Nutrition 0.000 description 1
- 229920001276 ammonium polyphosphate Polymers 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 235000011130 ammonium sulphate Nutrition 0.000 description 1
- 239000002956 ash Substances 0.000 description 1
- CSGLCWIAEFNDIL-UHFFFAOYSA-O azanium;urea;nitrate Chemical compound [NH4+].NC(N)=O.[O-][N+]([O-])=O CSGLCWIAEFNDIL-UHFFFAOYSA-O 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229920001222 biopolymer Polymers 0.000 description 1
- 229910052796 boron Inorganic materials 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 239000003054 catalyst Substances 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000009920 chelation Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 230000000536 complexating effect Effects 0.000 description 1
- 238000009833 condensation Methods 0.000 description 1
- 230000005494 condensation Effects 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- HNPSIPDUKPIQMN-UHFFFAOYSA-N dioxosilane;oxo(oxoalumanyloxy)alumane Chemical compound O=[Si]=O.O=[Al]O[Al]=O HNPSIPDUKPIQMN-UHFFFAOYSA-N 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 230000003311 flocculating effect Effects 0.000 description 1
- 125000000524 functional group Chemical group 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 238000000227 grinding Methods 0.000 description 1
- 239000008233 hard water Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 229910052748 manganese Inorganic materials 0.000 description 1
- 239000011572 manganese Substances 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 229910052750 molybdenum Inorganic materials 0.000 description 1
- 239000011733 molybdenum Substances 0.000 description 1
- 239000006012 monoammonium phosphate Substances 0.000 description 1
- 235000019837 monoammonium phosphate Nutrition 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 235000019796 monopotassium phosphate Nutrition 0.000 description 1
- 229910017604 nitric acid Inorganic materials 0.000 description 1
- 238000005121 nitriding Methods 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 230000001590 oxidative effect Effects 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 235000011007 phosphoric acid Nutrition 0.000 description 1
- 238000000053 physical method Methods 0.000 description 1
- 231100000572 poisoning Toxicity 0.000 description 1
- 230000000607 poisoning effect Effects 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 229910000027 potassium carbonate Inorganic materials 0.000 description 1
- 235000011181 potassium carbonates Nutrition 0.000 description 1
- 239000001508 potassium citrate Substances 0.000 description 1
- 229960002635 potassium citrate Drugs 0.000 description 1
- QEEAPRPFLLJWCF-UHFFFAOYSA-K potassium citrate (anhydrous) Chemical compound [K+].[K+].[K+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O QEEAPRPFLLJWCF-UHFFFAOYSA-K 0.000 description 1
- 235000011082 potassium citrates Nutrition 0.000 description 1
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 1
- WFIZEGIEIOHZCP-UHFFFAOYSA-M potassium formate Chemical compound [K+].[O-]C=O WFIZEGIEIOHZCP-UHFFFAOYSA-M 0.000 description 1
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 1
- AJTPODXVRWXEMS-UHFFFAOYSA-M potassium;4-(1h-indol-2-yl)butanoate Chemical compound [K+].C1=CC=C2NC(CCCC(=O)[O-])=CC2=C1 AJTPODXVRWXEMS-UHFFFAOYSA-M 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 201000010153 skin papilloma Diseases 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 235000012424 soybean oil Nutrition 0.000 description 1
- 239000003549 soybean oil Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000002623 sporogenic effect Effects 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 238000009210 therapy by ultrasound Methods 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 238000004383 yellowing Methods 0.000 description 1
- 239000010457 zeolite Substances 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/50—Treatments combining two or more different biological or biochemical treatments, e.g. anaerobic and aerobic treatment or vermicomposting and aerobic treatment
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Engineering & Computer Science (AREA)
- Pest Control & Pesticides (AREA)
- Soil Sciences (AREA)
- Tropical Medicine & Parasitology (AREA)
- Fertilizers (AREA)
Abstract
The invention provides a small molecular humic acid fertilizer and a preparation method thereof, wherein the preparation method comprises the following steps: (1) Mixing and culturing penicillium, streptomycete, bacillus, arthrobacter, streptoverticillium, lignite, a microorganism culture medium and water to obtain a preculture product; (2) Mixing and culturing the preculture product obtained in the step (1) with lignite and water to obtain small-molecular humic acid; (3) And (3) mixing the small-molecular humic acid obtained in the step (2) with a fertilizer raw material to obtain the small-molecular humic acid fertilizer. The small molecular humic acid fertilizer provided by the invention has high nutrient utilization rate, can promote the growth of crops, and can relieve the damage of hormone fertilizers to the crops.
Description
Technical Field
The invention belongs to the field of fertilizers, and particularly relates to a small-molecule humic acid fertilizer and a preparation method thereof, in particular to a small-molecule humic acid fertilizer with high nutrient utilization rate and a preparation method thereof.
Background
Humic acid is a macromolecular organic weak acid mixture with various functional groups, which is formed by decomposing and converting animal and plant remains through microorganisms and a series of changing processes of geochemistry and physics, plays an important role in plant growth, and leonardite is one of main sources of humic acid. Leonardite contains highly oxidized organic matter, various minerals accumulated throughout the year, sporogenic archaea, polysaccharides, biopolymers, enzymes, etc. The method for activating the leonardite humic acid by wind is more, and mainly comprises physical, chemical, biological and other methods, wherein the physical method mainly comprises mechanical crushing, ultrasonic treatment and other methods; the chemical method includes acid catalytic oxidation method, alkali dissolution acid precipitation method, and method of combining metal catalyst and acid catalytic oxidation method. The humic acid has larger molecular weight, poorer chelating ability and hard water resistance, is easier to form flocculating precipitation and the like, and has poorer effect on fertilizer nutrient synergism or on stimulating crop growth.
On the other hand, with the development of fertilizer industry, the application amount of the fertilizer is continuously increased, and plays an important role in promoting the continuous growth of agricultural production. But the problems of low fertilizer utilization rate and increased fertilizer adding cost are also commonly reflected. How to improve the utilization rate of chemical fertilizers is an important research subject of agricultural chemists. In the nitrogen, phosphorus, potassium and trace element fertilizer, a small amount of humic acid substances with chemical activity and biological activity are added, so that the utilization rate of the fertilizer can be improved to different degrees. A large number of researches prove that the chemical activity and biological activity humic acid substances are introduced into the fertilizer, and the chemical activity and biological activity humic acid substances have obvious effects of improving the utilization rate of the fertilizer through the actions of chemical combination, adsorption, chelation and the like of humic acid.
CN101024590B provides a preparation method of water-soluble fertilizer containing humic acid, in which the humic acid adopts potassium humate to replace sodium humate, the residue left after extracting potassium humate can be used for improving soil, avoiding pollution to environment, and the soil is supplemented with a large amount of element potassium, and the fertilizer contains a plurality of stable trace elements, thus being stable and comprehensive-nutrition humic acid fertilizer. The invention comprises the following operation steps: 1. adding water and raw materials containing humic acid into a pulp mixing tank, adding an extracting agent potassium hydroxide, maintaining the temperature between 50 and 60 ℃, and carrying out pulp mixing and extraction for 1 to 2 hours; centrifuging on a centrifuge, filtering potassium humate, and discharging residues through a conveyor belt; 2. dissolving raw materials such as nitrogen, phosphorus, potassium, boron, molybdenum and the like in water; 3. adding water into the complexing agent to dissolve, and then adding raw materials such as calcium, magnesium, copper, iron, zinc, manganese and the like into the complexing agent to carry out heating dissolution complexing; 4. mixing the materials prepared in the three steps in a finished product preparation tank, and emulsifying and grinding for 3-4 hours under the action of an emulsifying pump to obtain the finished product.
CN107337510a discloses a method for preparing a lignite humic acid activation and slow-release coated organic fertilizer, which comprises the steps of oxidizing lignite by nitric acid to improve the content of lignite humic acid, improving the quantity of carboxyl groups on the surface, improving the adsorptivity to ammonium ions, biologically activating lignite humic acid by using an enzyme preparation to make the lignite humic acid small in molecule, generating a series of condensation, oxidation, degradation and other reactions under the action of ammonia water, nitriding and dissolving out the lignite humic acid, proportioning and granulating the lignite humic acid with an organic decomposed fertilizer and an inorganic fertilizer, and coating the lignite humic acid in a coating agent prepared from gelatin, sodium cellulose, zeolite powder and soybean oil, so that the slow-release performance of the organic fertilizer is improved, fertilizer particles are stable, not easy to wet, easy to transport and the cost is reduced.
The problem of fertilizer utilization rate is also more serious because the demands for fertilizers are continuously increased at present, and humic acid has obvious effect on improving the effect of the fertilizers. Therefore, how to provide a fertilizer containing humic acid with high utilization rate becomes a problem to be solved urgently.
Disclosure of Invention
Aiming at the defects of the prior art, the invention aims to provide a small molecular humic acid fertilizer and a preparation method thereof, in particular to a small molecular humic acid fertilizer with high nutrient utilization rate and a preparation method thereof. The small molecular humic acid fertilizer provided by the invention has high nutrient utilization rate, can promote the growth of crops, and can relieve the damage of hormone fertilizers to the crops.
In order to achieve the aim of the invention, the invention adopts the following technical scheme:
in one aspect, the invention provides a preparation method of a small molecular humic acid fertilizer, which comprises the following steps:
(1) Mixing and culturing penicillium, streptomyces, bacillus, lignite, a microorganism culture medium and water to obtain a preculture product;
(2) Mixing and culturing the preculture product obtained in the step (1) with lignite and water to obtain small-molecular humic acid;
(3) And (3) mixing the small-molecular humic acid obtained in the step (2) with a fertilizer raw material to obtain the small-molecular humic acid fertilizer.
According to the method, by adopting the combination of the penicillium, the streptomyces, the bacillus, the arthrobacter and the streptoverticillium, the humic acid in the lignite can be effectively degraded into the micromolecular organic acid through the synergistic effect, the nutrient utilization rate of the fertilizer product is improved, the crop growth can be effectively promoted, the damage of the hormone fertilizer to the crop can be relieved, and the symptom of hormone toxicity can be relieved.
The invention does not limit the microorganism culture medium too much, can be purchased from the market, and can meet the requirement of strain growth and propagation.
Preferably, the mass ratio of the penicillium, the streptomyces, the bacillus, the arthrobacter and the streptoverticillium in the step (1) is (1.8-2.2), the mass ratio of the penicillium, the streptomyces, the bacillus, the arthrobacter and the streptoverticillium is (0.8-1.2), and the mass ratio of the penicillium is (2.8-3.2).
Preferably, the mass ratio of the total mass of the penicillium, the streptomyces, the bacillus, the arthrobacter and the streptoverticillium to the lignite mass in the step (1) is 1 (80-90).
The mass ratio of the penicillium, the streptomyces, the bacillus, the arthrobacter and the streptoverticillium can be 1.8:0.8:2.8:0.8:0.8, 2:1:3:1:1 or 2.2:1.2:3.2:1.2:1.2, and the like, and the mass ratio of the total mass of the penicillium, the streptomyces, the bacillus, the arthrobacter and the streptoverticillium to the mass of the lignite can be 1:80, 1:82, 1:54, 1:86, 1:88 or 1:90, and the like, but the mass ratio is not limited to the above-listed values, and other non-listed values in the numerical range are applicable.
Preferably, the penicillium in step (1) comprises any one of penicillium, penicillium nodosum or penicillium citrinum, preferably penicillium.
Preferably, the streptomyces of step (1) comprises any one of streptomyces griseus, streptomyces microflavus, streptomyces albus, streptomyces thermodurus or streptomyces albidogenus, preferably streptomyces microflavus or streptomyces albidogenus.
Preferably, the bacillus in step (1) comprises any one of bacillus licheniformis, bacillus subtilis, bacillus coagulans, bacillus mucilaginosus or bacillus polymyxa, preferably bacillus coagulans or bacillus mucilaginosus.
Preferably, the Arthrobacter in step (1) comprises any one of Arthrobacter globosum, arthrobacter gambogi, arthrobacter trophoblasti, arthrobacter alcalophilus or Arthrobacter sulphurous, preferably Arthrobacter globosum or Arthrobacter gambogi.
Preferably, the streptoverticillium in step (1) comprises streptoverticillium verrucosum or streptoverticillium verrucosum, preferably streptoverticillium verrucosum.
The preferred strain can further improve the effect of the fertilizer compared with other similar strains.
Preferably, the cultivation in step (1) is carried out at a temperature of 25-35 ℃ for 15-25 days, and the dissolved oxygen content in water is 5-8ppm.
Preferably, the mass ratio of the pre-culture product in the step (2) to the lignite is 1 (30-40).
Preferably, the culturing in step (2) is carried out at a temperature of 25-35℃for a period of 80-100 days.
Wherein the culture in the step (1) may be carried out at a temperature of 25 ℃, 26 ℃,27 ℃,28 ℃,29 ℃, 30 ℃, 31 ℃, 32 ℃,33 ℃, 34 ℃, 35 ℃ or the like, a time of 15 days, 16 days, 17 days, 18 days, 19 days, 20 days, 21 days, 22 days, 23 days, 24 days, 25 days or the like, an amount of dissolved oxygen of 5ppm, 6ppm, 7ppm, 8ppm or the like, a mass ratio of the preculture product in the step (2) to lignite of 1:30, 1:32, 1:34, 1:36, 1:38, 1:40 or the like, and the culture in the step (2) may be carried out at a temperature of 25 ℃, 26 ℃,27 ℃,28 ℃,29 ℃, 30 ℃, 31 ℃, 32 ℃,33 ℃, 34 ℃, 35 ℃ or the like, a time of 80 days, 82 days, 84 days, 86 days, 88 days, 90 days, 92 days, 94 days, 96 days, 98 days, 100 days or the like, but the values are not limited to the above, and the values are not applicable.
Preferably, the fertilizer raw material in step (3) includes any one or a combination of at least two of nitrogen fertilizer, phosphate fertilizer, potash fertilizer or trace element fertilizer, for example, a combination of nitrogen fertilizer and phosphate fertilizer, a combination of phosphate fertilizer and potash fertilizer or a combination of nitrogen fertilizer and potash fertilizer, etc., but not limited to the above-listed combinations, and other non-listed combinations within the above-listed combinations are equally applicable.
Illustratively, the nitrogen fertilizer may be selected from urea, ammonium sulfate, urea ammonium nitrate solution, etc., the phosphate fertilizer may be selected from phosphoric acid, monopotassium phosphate, monoammonium phosphate, ammonium polyphosphate, etc., the potash fertilizer may be selected from potassium carbonate, plant ash, potassium hydroxide, potassium formate, potassium citrate, etc., and the trace element fertilizer may be selected from EDTA chelating trace elements.
On the other hand, the invention also provides the small molecular humic acid fertilizer prepared by the preparation method.
Compared with the prior art, the invention has the following beneficial effects:
The invention provides a preparation method of a small molecular humic acid fertilizer, which is compounded by adopting penicillium, streptomyces, bacillus, arthrobacter and streptococci, and the synergistic effect can effectively degrade humic acid in lignite into small molecular organic acid, improve the nutrient utilization rate of the product fertilizer, effectively promote the growth of crops, relieve the damage of hormone fertilizer to crops and relieve hormone poisoning symptoms; and the effect of the fertilizer can be further improved compared with other strains of the same kind by screening the strains.
Detailed Description
In order to further describe the technical means adopted by the present invention and the effects thereof, the following describes the technical scheme of the present invention in combination with the preferred embodiments of the present invention, but the present invention is not limited to the scope of the embodiments.
In the following examples, all strains are from the industrial microorganism strain engineering research center of Henan province, a North sodium organism;
Lignite is from a weathered brown coal mine in arizona usa.
Example 1
The embodiment provides a small molecular humic acid fertilizer, which is prepared by the following steps:
(1) Mixing and culturing penicillium, streptomyces microflavus, bacillus coagulans, arthrobacter globosum, streptoverticillium verrucosum, lignite, a microorganism culture medium (rapeseed oil cake, bean pulp and sucrose) and water (the mass ratio of penicillium, streptomyces microflavus, bacillus coagulans, arthrobacter globosum and streptoverticillium verrucosum is 2:1:3:1:1), the total mass fraction of strains is 0.2%, the mass fraction of lignite is 16.8%, the mass fraction of rapeseed oil cake is 5%, the mass fraction of bean pulp is 10%, the mass fraction of sucrose is 8%, and the balance is water, wherein the temperature is 30 ℃ and the dissolved oxygen is 7ppm for 20 days, so as to obtain a preculture product;
(2) Mixing and culturing the preculture product obtained in the step (1) with lignite and water (the mass fraction of the preculture product is 1%, the mass fraction of the lignite is 36%, and the rest is water for 90 days at 30 ℃), so as to obtain micromolecular humic acid;
(3) And (3) mixing the small-molecular humic acid obtained in the step (2) with a fertilizer raw material to obtain the small-molecular humic acid fertilizer.
Example 2
The embodiment provides a small molecular humic acid fertilizer, which is prepared by the following steps:
(1) Mixing and culturing penicillium, streptomyces albidogenus, bacillus mucilaginosus, arthrobacter gambogi, streptoverticillium reevesii, lignite, a microorganism culture medium (rapeseed oil cake, bean pulp and sucrose) and water (the mass ratio of penicillium, streptomyces albidogenus, bacillus mucilaginosus, arthrobacter gambogi and streptoverticillium reevesii is 1.8:0.8:2.8:0.8:0.8:0.8), the total mass fraction of strains is 0.2%, the mass fraction of lignite is 16%, the mass fraction of rapeseed oil cake is 5%, the mass fraction of bean pulp is 10%, the mass fraction of sucrose is 8%, the rest is water, the temperature is 25 ℃ and the dissolved oxygen amount is 8 ppm), so as to obtain a preculture product;
(2) Mixing and culturing the preculture product obtained in the step (1), lignite, water and urea (the mass fraction of the preculture product is 1%, the mass fraction of the lignite is 30%, the mass fraction of the urea is 4%, and the rest is water, wherein the temperature is 25 ℃ for 100 days) to obtain micromolecular humic acid;
(3) And (3) mixing the small-molecular humic acid obtained in the step (2) with a fertilizer raw material to obtain the small-molecular humic acid fertilizer.
Example 3
The embodiment provides a small molecular humic acid fertilizer, which is prepared by the following steps:
(1) Mixing and culturing penicillium, streptomyces microflavus, bacillus coagulans, arthrobacter globosus, streptoverticillium verrucosum, lignite, a microorganism culture medium (rapeseed oil cake, soybean meal and sucrose) and water (the mass ratio of penicillium, streptomyces microflavus, bacillus coagulans, arthrobacter globosus and streptoverticillium verrucosum is 2.2:1.2:3.2:1.2:1.2:1.2), the total mass fraction of the strain is 0.2%, the mass fraction of lignite is 18%, the mass fraction of the rapeseed oil cake is 5%, the mass fraction of the soybean meal is 10%, the mass fraction of the sucrose is 8%, the rest is water, the temperature is 35 ℃ and the dissolved oxygen amount is 5ppm, so as to obtain a precultured product;
(2) Mixing and culturing the preculture product obtained in the step (1) with lignite and water (the mass fraction of the preculture product is 1%, the mass fraction of the lignite is 40%, and the rest is water for 80 days at the temperature of 35 ℃) to obtain micromolecular humic acid;
(3) And (3) mixing the small-molecular humic acid obtained in the step (2) with a fertilizer raw material to obtain the small-molecular humic acid fertilizer.
Example 4
This example provides a small molecule humic acid fertilizer, the process for preparation is the same as example 1 except that the penicillium is replaced with equivalent penicillium nodosum.
Example 5
The present example provides a small molecule humic acid fertilizer, the preparation method is the same as example 1 except that penicillium is replaced with equivalent penicillium citrinum.
Example 6
This example provides a small molecule humic acid fertilizer, which was prepared in the same manner as in example 1 except that Streptomyces microflavus was replaced with an equivalent amount of Streptomyces griseus.
Example 7
This example provides a small molecule humic acid fertilizer, which is prepared in the same manner as example 1 except that Streptomyces microflavus is replaced with equal amount of Streptomyces thermodurus.
Example 8
This example provides a small molecule humic acid fertilizer, which was prepared in the same manner as in example 1 except that Bacillus coagulans was replaced with equal amounts of Bacillus subtilis.
Example 9
This example provides a small molecule humic acid fertilizer, which was prepared in the same manner as in example 1 except that Bacillus coagulans was replaced with equal amount of Bacillus licheniformis.
Example 10
This example provides a small molecule humic acid fertilizer, the process for preparation is the same as example 1 except that Arthrobacter globosus is replaced with equal amount of Arthrobacter trophoblastus.
Example 11
This example provides a small molecule humic acid fertilizer, the process for preparation is the same as example 1 except that the streptoverticillium verrucosum is replaced with an equivalent amount of streptoverticillium verrucosum.
Comparative example 1
This comparative example provides a small molecule humic acid fertilizer, the preparation method is free from adding penicillium, and the reduction part is proportionally distributed to Streptomyces microflavus, bacillus coagulans, arthrobacter globosum and Alternaria verrucosa, and the rest is the same as in example 1.
Comparative example 2
This comparative example provides a small molecular humic acid fertilizer, the preparation method does not add Streptomyces microflavus, and the reduction part is proportionally distributed to Penicillium, bacillus coagulans, arthrobacter globosum, and Alternaria verrucosa, and the rest is the same as in example 1.
Comparative example 3
This comparative example provides a small molecule humic acid fertilizer, wherein Bacillus coagulans is not added in the preparation method, and the rest is the same as example 1 except that part of the small molecule humic acid fertilizer is proportionally distributed to Streptomyces microflavus, penicillium, arthrobacter globosum and Alternaria verrucosa.
Comparative example 4
This comparative example provides a small molecule humic acid fertilizer, which was prepared without adding Arthrobacter globosus, and the remainder was identical to example 1 except that a reduced proportion was allocated to Streptomyces microflavus, bacillus coagulans, penicillium, and Streptomyces verrucosus.
Comparative example 5
This comparative example provides a small molecule humic acid fertilizer, the preparation method does not add streptoverticillium wart, and the reduction part is distributed to streptomyces microflavus, bacillus coagulans, arthrobacter globosus and penicillium according to the proportion, and the rest is the same as that of example 1.
Comparative example 6
This comparative example provides a small molecule humic acid fertilizer, prepared in the same manner as example 1 except that the Arthrobacter was replaced with an equivalent amount of Mucor.
Comparative example 7
This comparative example provides a small molecule humic acid fertilizer prepared in the same manner as in example 1 except that the streptoverticillium is replaced with an equivalent amount of Sphingobacterium.
And (3) effect test:
Test site: yang Lingou Cui Gucun U horse test greenhouse of Shaanxi province.
Testing the product: active phosphorus of Shanghai's excellent horse products, ABT rooting powder, small molecular humic acid fertilizer provided in examples 1-11 and comparative examples 1-7.
The test crop is M26 apple tissue culture plug seedling, and is placed in a greenhouse for hardening off for 3 days before testing. The M26 apple tissue culture plug seedling 420 plants are equally divided into 21 groups, and are respectively treated with 300 times of active phosphorus, an ABT rooting powder aqueous solution (1 g/L), and 500 times of the small molecular humic acid fertilizer provided in examples 1-11 and comparative examples 1-7, and the rest group is firstly treated with the ABT rooting powder aqueous solution (1 g/L) and then treated with 500 times of the small molecular humic acid fertilizer (mixed treatment group); the treatment method of the 300 times liquid of active phosphorus and the 500 times liquid of the small molecular humic acid fertilizer is that the plant roots are soaked in the liquid for 30min and then taken out, and the treatment method of the ABT rooting powder aqueous solution is that the plants are dipped in the liquid for 30s and then taken out.
Transplanting the treated plants, wherein the plant row spacing is 20cm multiplied by 60cm, observing the change of overground parts, respectively measuring and calculating the transplanting survival rate, average plant height, total leaf number and average longest root length of apple seedlings on the 60 th day of transplanting, dividing the apple seedlings into 4 grades according to overground part growth vigor (plant height, leaf size, color and the like), and respectively counting the number and the ratio of the apple seedlings:
Class a: the leaves are all green, the leaves are large, and the plant height is more than 3cm;
B level: the leaves are all green, the leaves are smaller, and the plant height is less than 3cm;
C level: abnormal leaves, yellowing of the leaves and dry edges;
D stage: plants die.
The statistical results were as follows:
Meanwhile, the number of C-level plants in the rooting powder group and the mixed treatment group is observed at the 25 th day of transplanting, and the result is as follows:
| Group of | Number of C stages |
| Rooting powder group | 10 |
| Mixed treatment group | 4 |
The data show that the small molecular humic acid fertilizer prepared by the preparation method provided by the invention can effectively promote the crop growth due to the nutrient utilization rate of the fertilizer; as can be seen from the comparison of the example 1 and the comparative examples 1-5, the invention obviously improves the effect of promoting the growth of crops by adopting the combination of the five species of penicillium, streptomyces, bacillus, arthrobacter and streptoverticillium, and has the advantages of higher plants, larger average leaf number, longer average longest root length, better plant growth vigor and higher class A occupation ratio; comparing examples 1 and 4-11, it can be found that the invention can further improve the effect of the product compared with other strains by optimizing specific strains; as can be seen from the comparison of example 1 and comparative examples 6 to 7, the present invention can significantly improve the effect of the product by using a specific strain compared to other strain combinations; compared with the conventional fertilizer, the invention can further improve the growth state of plants, and can find that the rooting powder group has the greatest damage to the plants, the C-level proportion is the greatest, the main components of the ABT rooting powder are potassium indolebutyrate and sodium naphthylacetate, the M26 apple rootstock is easily influenced by the hormone concentration, and the C-level quantity can be found when the M26 apple rootstock is transplanted on 25 days.
The applicant states that the present invention is illustrated by the above examples as a small molecule humic acid fertilizer and a method for preparing the same, but the present invention is not limited to the above examples, i.e. it is not meant that the present invention must be practiced by relying on the above examples. It should be apparent to those skilled in the art that any modification of the present invention, equivalent substitution of raw materials for the product of the present invention, addition of auxiliary components, selection of specific modes, etc., falls within the scope of the present invention and the scope of disclosure.
The preferred embodiments of the present invention have been described in detail above, but the present invention is not limited to the specific details of the above embodiments, and various simple modifications can be made to the technical solution of the present invention within the scope of the technical concept of the present invention, and all the simple modifications belong to the protection scope of the present invention.
In addition, the specific features described in the above embodiments may be combined in any suitable manner, and in order to avoid unnecessary repetition, various possible combinations are not described further.
Claims (16)
1. The preparation method of the small molecular humic acid fertilizer is characterized by comprising the following steps of:
(1) Mixing and culturing penicillium, streptomycete, bacillus, arthrobacter, streptoverticillium, lignite, a microorganism culture medium and water to obtain a preculture product;
(2) Mixing and culturing the preculture product obtained in the step (1) with lignite and water to obtain small-molecular humic acid;
(3) Mixing the small molecular humic acid obtained in the step (2) with a fertilizer raw material to obtain the small molecular humic acid fertilizer;
the mass ratio of the penicillium, the streptomyces, the bacillus, the arthrobacter and the streptoverticillium in the step (1) is (1.8-2.2), is (0.8-1.2), is (2.8-3.2), is (0.8-1.2), and the mass ratio of the total mass of the penicillium, the streptomyces, the bacillus, the arthrobacter and the streptoverticillium to the mass of the lignite is 1 (80-90).
2. The method of claim 1, wherein the penicillium in step (1) comprises any one of penicillium, penicillium nodosum or penicillium citrinum.
3. The method according to claim 2, wherein the penicillium in step (1) is penicillium.
4. The method according to claim 1, wherein the Streptomyces in step (1) comprises any one of Streptomyces griseus, streptomyces microflavus, streptomyces albus, streptomyces thermodurus or Streptomyces albilineans.
5. The method according to claim 4, wherein the Streptomyces in step (1) is Streptomyces microflavus or Streptomyces albus.
6. The method according to claim 1, wherein the bacillus in the step (1) comprises any one of bacillus licheniformis, bacillus subtilis, bacillus coagulans, bacillus mucilaginosus and bacillus polymyxa.
7. The method according to claim 6, wherein the bacillus in the step (1) is Bacillus coagulans or Bacillus mucilaginosus.
8. The method according to claim 1, wherein the Arthrobacter in step (1) comprises any one of Arthrobacter globosum, arthrobacter gambogi, arthrobacter trophoblastus, arthrobacter alcaligenes and Arthrobacter sulphurous.
9. The method according to claim 8, wherein the Arthrobacter in step (1) is Arthrobacter globosum or Arthrobacter gambogi.
10. The method of claim 1, wherein the streptoverticillium in step (1) comprises streptoverticillium verrucosum or streptoverticillium verrucosum.
11. The method of claim 10, wherein the streptoverticillium in step (1) is streptoverticillium verrucosum.
12. The method according to claim 1, wherein the culturing in the step (1) is performed at a temperature of 25 to 35℃for 15 to 25 days, and the dissolved oxygen amount in water is 5 to 8ppm.
13. The method according to claim 1, wherein the mass ratio of the preculture product to lignite in step (2) is 1 (30-40).
14. The method according to claim 1, wherein the culturing in the step (2) is performed at a temperature of 25 to 35℃for 80 to 100 days.
15. The method of claim 1, wherein the fertilizer material of step (3) comprises any one or a combination of at least two of nitrogen fertilizer, phosphate fertilizer, potash fertilizer, or trace element fertilizer.
16. A small molecule humic acid fertilizer prepared by the method of any one of claims 1 to 15.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1081173A (en) * | 1993-03-07 | 1994-01-26 | 钟显亮 | Brown coal microbial fertilizer and manufacture method thereof |
| CN105801311A (en) * | 2016-04-29 | 2016-07-27 | 潍坊英雷生物科技有限公司 | Preparation technology of humic acid compound fertilizer |
| CN108002943A (en) * | 2017-09-28 | 2018-05-08 | 上海芳甸生物科技有限公司 | Compound microorganism ferments type soil conditioner |
| CN108218615A (en) * | 2017-12-25 | 2018-06-29 | 云南云叶化肥股份有限公司 | A kind of organic and inorganic humic acid fertilizer containing active microorganism and preparation method thereof |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1081173A (en) * | 1993-03-07 | 1994-01-26 | 钟显亮 | Brown coal microbial fertilizer and manufacture method thereof |
| CN105801311A (en) * | 2016-04-29 | 2016-07-27 | 潍坊英雷生物科技有限公司 | Preparation technology of humic acid compound fertilizer |
| CN108002943A (en) * | 2017-09-28 | 2018-05-08 | 上海芳甸生物科技有限公司 | Compound microorganism ferments type soil conditioner |
| CN108218615A (en) * | 2017-12-25 | 2018-06-29 | 云南云叶化肥股份有限公司 | A kind of organic and inorganic humic acid fertilizer containing active microorganism and preparation method thereof |
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