CN115251244A - Slow-release antibacterial feed additive and preparation method and application thereof - Google Patents
Slow-release antibacterial feed additive and preparation method and application thereof Download PDFInfo
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- CN115251244A CN115251244A CN202210967940.2A CN202210967940A CN115251244A CN 115251244 A CN115251244 A CN 115251244A CN 202210967940 A CN202210967940 A CN 202210967940A CN 115251244 A CN115251244 A CN 115251244A
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- 239000003674 animal food additive Substances 0.000 title claims abstract description 69
- 230000000844 anti-bacterial effect Effects 0.000 title claims abstract description 40
- 238000002360 preparation method Methods 0.000 title claims abstract description 24
- WBYWAXJHAXSJNI-VOTSOKGWSA-M trans-cinnamate Chemical class [O-]C(=O)\C=C\C1=CC=CC=C1 WBYWAXJHAXSJNI-VOTSOKGWSA-M 0.000 claims abstract description 37
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- 239000001405 butyl (E)-3-phenylprop-2-enoate Substances 0.000 claims abstract description 16
- OHHIVLJVBNCSHV-KTKRTIGZSA-N butyl cinnamate Chemical compound CCCCOC(=O)\C=C/C1=CC=CC=C1 OHHIVLJVBNCSHV-KTKRTIGZSA-N 0.000 claims abstract description 16
- CCRCUPLGCSFEDV-UHFFFAOYSA-N cinnamic acid methyl ester Natural products COC(=O)C=CC1=CC=CC=C1 CCRCUPLGCSFEDV-UHFFFAOYSA-N 0.000 claims abstract description 16
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Images
Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/111—Aromatic compounds
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/158—Fatty acids; Fats; Products containing oils or fats
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K40/00—Shaping or working-up of animal feeding-stuffs
- A23K40/30—Shaping or working-up of animal feeding-stuffs by encapsulating; by coating
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/60—Feeding-stuffs specially adapted for particular animals for weanlings
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/21—Esters, e.g. nitroglycerine, selenocyanates
- A61K31/215—Esters, e.g. nitroglycerine, selenocyanates of carboxylic acids
- A61K31/216—Esters, e.g. nitroglycerine, selenocyanates of carboxylic acids of acids having aromatic rings, e.g. benactizyne, clofibrate
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
- A61K47/34—Macromolecular compounds obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyesters, polyamino acids, polysiloxanes, polyphosphazines, copolymers of polyalkylene glycol or poloxamers
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
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- A61P1/12—Antidiarrhoeals
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- A—HUMAN NECESSITIES
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Abstract
The invention relates to the technical field of feed additives, and particularly discloses a slow-release antibacterial feed additive and a preparation method and application thereof. The slow-release antibacterial feed additive comprises the following components in parts by weight: 60-80 parts of cinnamate compounds, 10-20 parts of hydrogenated vegetable oil, 5-10 parts of sodium alginate and 1-5 parts of polyethylene glycol glyceryl ricinoleate, wherein the cinnamate compounds are a composition of methyl cinnamate, ethyl cinnamate, propyl cinnamate and butyl cinnamate. The slow-release antibacterial feed additive provided by the invention is not easy to decompose by gastric acid, can smoothly reach the intestinal tract of animals, has the effects of killing intestinal pathogenic bacteria and diminishing inflammation, is beneficial to promoting the healthy growth of the animals and preventing the animals from diarrhea, can be added into piglet feed as a substitute of antibiotics, prevents piglet diarrhea, improves the growth speed of piglets, and keeps the high survival rate of the piglets.
Description
Technical Field
The invention relates to the technical field of feed additives, in particular to a slow-release antibacterial feed additive and a preparation method and application thereof.
Background
In the 20 th century or around the 50's, antibiotics were found to have the effect of increasing the average daily gain and feed conversion ratio of livestock, whereby growth-promoting antibiotics for feed were largely used for improving the productivity of livestock. In addition, because antibiotics also have excellent antibacterial property, in order to prevent and treat diarrhea of piglets, some feed producers or farmers excessively add or abuse antibiotics in the feed, so that intestinal flora disturbance of piglets is caused, drug-resistant strains are generated, and no drug can be cured when diarrhea occurs. Overdosing or overdosing can also leave the antibiotic enriched in the animal and transported through the food chain into the human body, creating food safety issues. Meanwhile, antibiotics are discharged to the environment through animal wastes, and environmental hazards are caused.
Aiming at the problems, the national agricultural and rural bureau issues No. 194 bulletin, and the production of the feed containing the growth-promoting drug feed additive is required to be stopped from 7 months and 1 day in 2020. Therefore, there is a need for a feed additive that can replace antibiotics.
Disclosure of Invention
The invention aims to provide a slow-release antibacterial feed additive and a preparation method thereof, wherein the slow-release antibacterial feed additive has the effects of killing intestinal pathogenic bacteria and diminishing inflammation, and is beneficial to promoting the healthy growth of animals and preventing the animals from diarrhea. The invention also aims to provide a new application of the slow-release antibacterial feed additive, and particularly relates to a feed for preventing piglet diarrhea.
In order to realize the purpose, the invention adopts the technical scheme that:
firstly, the invention adopts the cinnamate compounds with specific component formula as the active ingredients of the feed additive. The cinnamate compounds are phenylpropanoid compounds, are widely distributed in nature, are easy to obtain raw materials, and have good activity in the aspects of bacteriostasis, disinsection, tumor resistance, oxidation resistance and the like. However, the cinnamate compounds are easily decomposed by gastric acid, and if they are added directly, they are difficult to reach the intestinal tracts of animals and do not work.
Therefore, the invention adopts hydrogenated vegetable oil and sodium alginate as coating materials to coat the cinnamate compounds. The hydrogenated vegetable oil can be used as a coating auxiliary agent in a controlled release preparation, and can slow down the sedimentation of components in the preparation of a compound preparation with larger specific gravity difference, avoid the uneven distribution of the preparation components and improve the solidification process. Meanwhile, the hydrogenated vegetable oil also has the functions of thickening, hardening, slow release and the like in the preparation. Sodium alginate is a natural polysaccharide, has the stability, solubility, viscosity and safety required by pharmaceutical formulation excipients, and has the ability to concentrate solutions, form gels and films.
Meanwhile, polyethylene glycol glyceryl ricinoleate which is a special fat and belongs to a hydrophilic emulsifier is added into the formula, so that the rapid dispersion of the substances in water can be promoted. The addition of the polyethylene glycol glyceryl ricinoleate can lead the cinnamate compounds to be rapidly dispersed after reaching the intestines and stomach of animals, and directly act on harmful bacteria.
The combined action of the hydrogenated vegetable oil, the sodium alginate and the polyethylene glycol glyceryl ricinoleate can form an effective coating slow-release effect on the cinnamate compounds, prevent the cinnamate compounds from being decomposed by gastric acid, enable the cinnamate compounds to smoothly reach intestinal tracts, rapidly disperse in the intestinal tracts and play an antibacterial role.
The inventor also finds that the dosage of the components and the preparation process directly influence the efficacy of the additive in the experimental research process. Therefore, the invention provides a formula and a preparation method of the feed additive capable of effectively preventing the diarrhea of the piglets.
A slow-release antibacterial feed additive comprises the following components in parts by weight: 60-80 parts of cinnamate compounds, 10-20 parts of hydrogenated vegetable oil, 5-10 parts of sodium alginate and 1-5 parts of polyethylene glycol glycerol ricinoleate, wherein the cinnamate compounds are a composition of methyl cinnamate, ethyl cinnamate, propyl cinnamate and butyl cinnamate.
Preferably, the weight ratio of the methyl cinnamate to the ethyl cinnamate to the propyl cinnamate to the butyl cinnamate is 1. The inventor finds that the effect of the combination of the four cinnamate compounds on preventing piglet diarrhea is obviously improved when the four cinnamate compounds are used in combination.
Preferably, the hydrogenated vegetable oil is selected from at least one of hydrogenated soybean oil and hydrogenated castor oil.
The preparation method of the slow-release antibacterial feed additive comprises the following steps:
(1) Putting hydrogenated vegetable oil, sodium alginate and polyethylene glycol glyceryl ricinoleate into an emulsification tank, heating and stirring until the solid is molten into a liquid state;
(2) Putting the cinnamate compound into the emulsifying tank in the step (1), heating and stirring until the liquid is in a uniform state, and then homogenizing;
(3) Pumping the material treated in the step (2) into a cooling spray granulation tower from an emulsifying tank, and cold spraying the material to obtain a finished product.
Preferably, the heating temperature of the step (1) and the step (2) is 70-80 ℃. At this heating temperature, not only can the components be effectively dispersed to achieve coating, but also the activity of the active ingredients is not destroyed.
Preferably, the rotation speed of the homogenizing treatment is 3000-4000RPM.
The invention also provides a new application of the slow-release antibacterial feed additive, in particular to an application in preparing feed. Preferably, the feed is a piglet feed. The slow-release antibacterial feed additive can effectively prevent the piglets from diarrhea, improve the growth speed of the piglets and keep the high survival rate of the piglets.
The invention also provides a piglet feed which contains the slow-release antibacterial feed additive. Preferably, every 1000 kg of the piglet feed contains 500 g of the slow-release antibacterial feed additive. The slow-release antibacterial feed additive is used according to the addition amount of the feed additive, so that the diarrhea of piglets can be effectively prevented.
Has the advantages that: the slow-release antibacterial feed additive provided by the invention is not easily decomposed by gastric acid, can smoothly reach the intestinal tract of animals, has the effects of killing intestinal pathogenic bacteria and diminishing inflammation, is favorable for promoting the healthy growth of the animals and preventing the diarrhea of the animals, can be added into piglet feed as a substitute of antibiotics, prevents the diarrhea of piglets, improves the growth speed of the piglets, and keeps the high survival rate of the piglets.
Drawings
FIG. 1 shows the results of the in vitro bacteriostatic test in example 1.
Detailed Description
To better illustrate the objects, aspects and advantages of the present invention, the present invention is further illustrated by the following examples. It is apparent that the following examples are only some of the examples of the present invention, and not all of them. It should be understood that the embodiments of the present invention are only for illustrating the technical effects of the present invention, and are not intended to limit the scope of the present invention.
Example 1
The embodiment provides a slow-release antibacterial feed additive for preventing piglet diarrhea, which comprises the following components in parts by weight: the high-efficiency and high-efficiency oil-free environment-friendly composite material comprises 60 parts of cinnamate compounds, 10 parts of hydrogenated vegetable oil, 5 parts of sodium alginate and 1 part of polyethylene glycol glyceryl ricinoleate, wherein the cinnamate compounds comprise methyl cinnamate, ethyl cinnamate, propyl cinnamate and butyl cinnamate in a mass ratio of 1.
The preparation method of the slow-release antibacterial feed additive comprises the following steps:
(1) Putting hydrogenated vegetable oil, sodium alginate and polyethylene glycol glyceryl ricinoleate into an emulsification tank, heating to 70-80 ℃, and uniformly stirring until the solid is molten into a liquid state;
(2) Putting the cinnamate compound into the emulsifying tank in the step (1), stirring the cinnamate compound at the temperature of 70-80 ℃ until the liquid is in a uniform state, and then carrying out homogenization treatment at the rotating speed of 3000-4000RPM;
(3) And after homogenizing, pumping the material into a cooling spray granulation tower from an emulsifying tank, and cold spraying the material to obtain the slow-release antibacterial feed additive.
Example 2
The embodiment provides a slow-release antibacterial feed additive for preventing piglet diarrhea, which comprises the following components in parts by weight: the high-performance composite oil is prepared from 70 parts of cinnamate compounds, 15 parts of hydrogenated vegetable oil, 8 parts of sodium alginate and 3 parts of polyethylene glycol glyceryl ricinoleate, wherein the cinnamate compounds comprise methyl cinnamate, ethyl cinnamate, propyl cinnamate and butyl cinnamate in a mass ratio of 1.
The preparation method of the slow-release antibacterial feed additive is the same as that of the example 1.
Example 3
The embodiment provides a slow-release antibacterial feed additive for preventing piglet diarrhea, which comprises the following components in parts by weight: 80 parts of cinnamate compounds, 20 parts of hydrogenated vegetable oil, 10 parts of sodium alginate and 5 parts of polyethylene glycol glyceryl ricinoleate, wherein, the cinnamate compounds comprise methyl cinnamate, ethyl cinnamate, propyl cinnamate and butyl cinnamate in a mass ratio of 1.
The preparation method of the slow-release antibacterial feed additive is the same as that of the example 1.
Example 4
This example provides a slow-release antibacterial feed additive for preventing diarrhea in piglets, which is different from example 1 only in the component ratio of the cinnamate compounds.
In example 4, the weight ratio of the components of the cinnamate compound to the methyl cinnamate to the ethyl cinnamate to the propyl cinnamate to the butyl cinnamate = 2.
Example 5
This example provides a slow-release antibacterial feed additive for preventing diarrhea in piglets, which is different from example 1 only in the component ratio of cinnamate compounds.
In example 5, the weight ratio of the components of the cinnamate compound to the methyl cinnamate to the ethyl cinnamate to the propyl cinnamate to the butyl cinnamate = 1.
Comparative example 1
A feed additive comprises the following components in parts by weight: 60 parts of cinnamate compounds, 3 parts of hydrogenated vegetable oil, 12 parts of sodium alginate and 1 part of polyethylene glycol glyceryl ricinoleate, wherein, the cinnamate compound comprises methyl cinnamate, ethyl cinnamate, propyl cinnamate and butyl cinnamate in a mass ratio of 1. The preparation method of the feed additive refers to the preparation method of example 1.
Comparative example 2
A feed additive comprises the following components in parts by weight: the formula comprises 60 parts of cinnamate compounds, 10 parts of hydrogenated vegetable oil and 1 part of polyethylene glycol glyceryl ricinoleate, wherein the cinnamate compounds comprise methyl cinnamate, ethyl cinnamate, propyl cinnamate and butyl cinnamate in a mass ratio of 1. The preparation method of the feed additive refers to the preparation method of example 1.
Comparative example 3
A feed additive comprises the following components in parts by weight: the high-performance and high-performance hydrogenated vegetable oil is prepared from 60 parts of cinnamate compounds, 5 parts of sodium alginate and 1 part of polyethylene glycol glyceryl ricinoleate, wherein the cinnamate compounds comprise methyl cinnamate, ethyl cinnamate, propyl cinnamate and butyl cinnamate in a mass ratio of 1. The preparation method of the feed additive refers to the preparation method of example 1.
Comparative example 4
A feed additive comprises the following components in parts by weight: the high-performance and high-performance composite oil is characterized by comprising 60 parts of cinnamate compounds, 10 parts of hydrogenated vegetable oil and 5 parts of sodium alginate, wherein the cinnamate compounds comprise methyl cinnamate, ethyl cinnamate, propyl cinnamate and butyl cinnamate in a mass ratio of 1. The preparation method of the feed additive refers to the preparation method of example 1.
Comparative example 5
A feed additive which is different from example 1 only in the composition of the components of the cinnamate-based compound.
In a comparative example 5, the cinnamate compound is composed of methyl cinnamate and ethyl cinnamate in a mass ratio of 1.
Comparative example 6
A feed additive which differs from example 1 only in the composition of the components of the cinnamate-based compound.
In comparative example 6, the cinnamate-based compound was composed of methyl cinnamate and butyl cinnamate in a mass ratio of 1.
Comparative example 7
A feed additive which is different from example 1 only in the composition of the components of the cinnamate-based compound.
In comparative example 7, the cinnamate-based compound was composed of propyl cinnamate and butyl cinnamate in a mass ratio of 1.
Test examples
1. In vitro bacteriostasis test
The test method comprises the following steps:
coli as a test bacterium, 4 groups of culture media are prepared, each group comprises 3, and the serial numbers of each group of culture media are respectively as follows: no. 2, no. 4, no. 6 and "culture + bacteria". Coli was inoculated on each medium, wherein the medium of group 2 was supplemented with aureomycin at a concentration of 75ppm, the medium of group 4 was supplemented with oxytetracycline at a concentration of 300ppm, the medium of group 6 was supplemented with the sustained-release antibacterial feed additive of example 1 at a concentration of 500ppm, and the culture group was a blank control group. Coli growth was observed at 0h, 2h and 3h after the start of the experiment in each group of the medium, and the results are shown in FIG. 1.
As can be seen from fig. 1, group 6 had excellent antibacterial effects, and the effects were superior to those of group 2 using aureomycin and group 4 using oxytetracycline.
2. Effect test for prevention of diarrhea in piglets
Selecting 130 healthy weaned piglets 24 + -2 days old, and randomly dividing into 13 groups according to the principle that the sources are consistent, and the weights are consistent according to the proportion of the proximal sex and the sex, wherein 12 groups are test groups, and 1 group is a control group. The piglets are fed and drunk freely, the house temperature is controlled to be 26-28 ℃, the relative humidity in the house is 68-80%, and the rest is carried out according to the normal feeding management of a pig farm.
300ppm of oxytetracycline and 75ppm of chlortetracycline are added into the feed of a control group according to the formula of a pig farm for basic daily ration. The daily ration of the test group is not added with antibiotics, but each ton of feed is added with 0.5kg of feed additive, and the feed additive specifically comprises the following components: test group 1 added with the feed additive of example 1, test group 2 added with the feed additive of example 2, test group 3 added with the feed additive of example 3, test group 4 added with the feed additive of example 4, test group 5 added with the feed additive of example 5, test group 6 added with the feed additive of comparative example 1, test group 7 added with the feed additive of comparative example 2, test group 8 added with the feed additive of comparative example 3, test group 9 added with the feed additive of comparative example 4, test group 10 added with the feed additive of comparative example 5, test group 11 added with the feed additive of comparative example 6, and test group 12 added with the feed additive of comparative example 7. The pigs are fed continuously for 14 days, and diarrhea index records are carried out on the pigs in a test period of 9, 13 and 17. At the same time, the average weight of each group at the beginning and end of the test was recorded. The test results are shown in Table 2.
TABLE 1 diarrhea index score Table (Chen dynasty, 1995)
TABLE 2 test results for prevention of diarrhoea in piglets
The test result shows that: the slow-release antibacterial feed additive can greatly reduce piglet diarrhea, improve the health condition of a swinery, increase the growth speed of piglets and keep the high survival rate of piglets. The concrete expression is as follows: 1. antibiotics are not used, the diarrhea of the piglets can be prevented, and the high survival rate of the piglets is kept; 2. the material-weight ratio can be obviously reduced; 3. can obviously improve the daily gain of the piglets.
Finally, it should be noted that the above embodiments are only used for illustrating the technical solutions of the present invention and not for limiting the protection scope of the present invention, and although the present invention is described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions can be made on the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention.
Claims (10)
1. The slow-release antibacterial feed additive is characterized by comprising the following components in parts by weight: 60-80 parts of cinnamate compounds, 10-20 parts of hydrogenated vegetable oil, 5-10 parts of sodium alginate and 1-5 parts of polyethylene glycol glyceryl ricinoleate, wherein the cinnamate compounds are a composition of methyl cinnamate, ethyl cinnamate, propyl cinnamate and butyl cinnamate.
2. The slow-release antibacterial feed additive as claimed in claim 1, wherein the weight ratio of the components of the cinnamate compounds is methyl cinnamate to ethyl cinnamate to propyl cinnamate to butyl cinnamate = 1.
3. The sustained-release antibacterial feed additive according to claim 1, wherein the hydrogenated vegetable oil is at least one selected from the group consisting of hydrogenated soybean oil and hydrogenated castor oil.
4. A method for preparing a sustained-release antibacterial feed additive according to any one of claims 1 to 3, comprising the steps of:
(1) Putting hydrogenated vegetable oil, sodium alginate and polyethylene glycol glyceryl ricinoleate into an emulsification tank, heating and stirring until the solid is molten into a liquid state;
(2) Putting the cinnamate compound into the emulsifying tank in the step (1), heating and stirring until the liquid is in a uniform state, and then homogenizing;
(3) Pumping the material treated in the step (2) into a cooling spray granulation tower from an emulsifying tank, and performing cold spraying to obtain a finished product.
5. The method for preparing a sustained-release antibacterial feed additive according to claim 4, wherein the heating temperature in the step (1) and the step (2) is 70 to 80 ℃.
6. The method for preparing a sustained-release antibacterial feed additive according to claim 4, wherein the rotation speed of the homogenizing treatment is 3000-4000RPM.
7. Use of a sustained-release antibacterial feed additive according to any one of claims 1 to 3 for the preparation of a feed.
8. The use according to claim 7, wherein the feed is a piglet feed.
9. A piglet feed comprising the sustained-release antibacterial feed additive according to any one of claims 1 to 3.
10. The piglet feed according to claim 9, wherein 500 g of the slow-release antibacterial feed additive is contained per 1000 kg of the piglet feed.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111011634A (en) * | 2019-12-31 | 2020-04-17 | 广东汇海农牧科技集团有限公司 | Pig feed additive for preventing diarrhea of weaned piglets and pig feed |
| CN111345402A (en) * | 2020-03-25 | 2020-06-30 | 广州市优百特饲料科技有限公司 | Functional additive and preparation method and application thereof |
| US20210244048A1 (en) * | 2020-02-11 | 2021-08-12 | Hangzhou Longyu Bio-Technology Co., Ltd. | Feed additive and application thereof |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111011634A (en) * | 2019-12-31 | 2020-04-17 | 广东汇海农牧科技集团有限公司 | Pig feed additive for preventing diarrhea of weaned piglets and pig feed |
| US20210244048A1 (en) * | 2020-02-11 | 2021-08-12 | Hangzhou Longyu Bio-Technology Co., Ltd. | Feed additive and application thereof |
| CN111345402A (en) * | 2020-03-25 | 2020-06-30 | 广州市优百特饲料科技有限公司 | Functional additive and preparation method and application thereof |
Non-Patent Citations (1)
| Title |
|---|
| 范芳;等: "肉桂酸丙酯抑菌活性的研究", 河南农业科学, no. 11, pages 164 - 166 * |
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