CN115250914A - Seedling growing method using simple greenhouse to replace sealwort cultivation room - Google Patents
Seedling growing method using simple greenhouse to replace sealwort cultivation room Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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Abstract
A seedling raising method using a simple greenhouse to replace a polygonatum sibiricum culture room comprises the following steps: 1) Selecting and pretreating rhizoma polygonati; 2) And (3) proliferation culture: subpackaging the explant into a proliferation culture bottle, then placing the proliferation culture bottle in a simple greenhouse for proliferation culture to obtain a proliferation seedling, wherein the proliferation culture is sequentially subjected to dark culture treatment in a sprouting and branching stage and illumination culture treatment in a leaf unfolding stage of the explant; 3) Rooting culture: and (5) carrying out rooting culture on the proliferated seedlings in a simple greenhouse to obtain rooted seedlings. The seedling culture method is ideal in design, changes the traditional sealwort seedling culture mode, adopts a simple greenhouse to replace a sealwort culture room, namely adopts natural solar illumination as energy, so that energy can be saved, the seedling culture cost is low, the sealwort tissue culture seedlings can grow more robustly when receiving sunlight, more importantly, the adaptability of the sealwort tissue culture seedlings to the external environment is enhanced, and the sealwort tissue culture seedlings are easy to survive in later domestication.
Description
Technical Field
The invention relates to a plant tissue culture technology, in particular to a seedling culture method using a simple greenhouse to replace a sealwort culture room.
Background
The Polygonatum sibiricum is a perennial herb of Polygonatum of Liliaceae, and has a medicinal history of more than two thousand years. Many varieties of sealwort are provided, tubers of three types of sealwort, polygonatum kingianum and sealwort are specified in the 2020 edition of the pharmacopoeia of the people's republic of China as crude drugs, wherein the quality of sealwort (Polygonatum cyrtonema Hua) is the best, and the sealwort mainly contains saccharides, steroid saponin, flavone and anthraquinone compounds, alkaloid, cardiac glycoside, lignan, vitamins, various amino acids and trace elements which are useful for human bodies and the like, has the effects of prolonging the life and resisting aging, influencing the cardiovascular system, regulating the immunity, resisting inflammation and pathogenic microorganisms, resisting fatigue, improving the learning memory, inhibiting tumor cells and the like, and is safe, non-toxic and non-mutagenic. With the gradual and deep research of people on pharmacology and pharmacotization of polygonatum, the polygonatum is more and more widely applied in the aspects of clinical application, health products, foods, cosmetics, ornamental value and the like.
In the existing production of sealwort tissue culture seedlings, the production needs to be carried out in a constant temperature culture room, so that the automatic control of indoor temperature (namely air conditioning) and illumination (namely lamp tube) can be realized, and the requirements of sealwort on environmental temperature and illumination are met. However, the sealwort tissue culture seedlings cultured in the existing constant-temperature culture room lack the adaptability to the external environment, and the survival rate of later-stage planting is influenced; and the culture cost is high (for example, the electricity charge of a lamp tube air conditioner is greatly consumed) by adopting a constant-temperature culture room culture mode, and common seedling raising producers are difficult to bear due to the pressure in the culture cost, and are difficult to popularize and use on a large scale in a short time.
Disclosure of Invention
The invention provides a seedling culture method by using a simple greenhouse to replace a polygonatum sibiricum culture room, and aims to overcome the defects that the existing seedling culture method of polygonatum sibiricum tissue culture seedlings is not ideal enough, lacks the adaptability to the external environment, influences the survival rate of later-stage planting, has high seedling culture cost and the like.
In order to solve the technical problems, the technical scheme of the invention is as follows:
a seedling raising method using a simple greenhouse to replace a polygonatum sibiricum culture room comprises the following steps: 1) Selecting and pretreating rhizoma polygonati, namely selecting underground rhizomes of the rhizoma polygonati, and pretreating the underground rhizomes to serve as explants; 2) And (3) proliferation culture: subpackaging the explant into proliferation culture bottles, then placing the proliferation culture bottles in a simple greenhouse for proliferation culture to obtain proliferation seedlings, wherein the proliferation culture is sequentially subjected to dark culture treatment in a sprouting and branching stage and illumination culture treatment in a leaf unfolding stage of the explant, and the illumination intensity of the illumination culture treatment is controlled to be 3000-5000 lx; 3) Rooting culture: and (3) carrying out rooting culture on the proliferated seedlings in a simple greenhouse to obtain rooted seedlings, wherein the illumination intensity in the simple greenhouse is controlled to be 0.5-5 kalx, and the temperature in the simple greenhouse is controlled to be 20-35 ℃.
Furthermore, the simple greenhouse comprises a greenhouse frame and an interlayer which divides the greenhouse frame into an upper layer and a lower layer, wherein the lower layer is used for multiplication culture of explants, and the upper layer is used for rooting culture of multiplication seedlings.
Furthermore, the interlayer is a light-transmitting partition plate with a first sun-shading net capable of being pulled open, the inner wall of the shed frame is provided with a first sun-shading net and a second sun-shading net capable of being pulled open, and the first sun-shading net and the second sun-shading net are positioned on the left side wall and the right side wall of the lower layer.
Furthermore, the upper layer is provided with an upper culture frame for rooting culture of the proliferated seedlings; the lower layer is provided with a lower culture frame for multiplication culture of explants; and a ladder for a worker to ascend to the upper layer is arranged in the shed frame.
Furthermore, a heating device for adjusting the temperature and humidity in the shed frame is further arranged in the shed frame.
The simple greenhouse further comprises a greenhouse frame, and a first vertical interlayer and a second vertical interlayer which divide the greenhouse frame into a front space, a middle space and a rear space in sequence from front to back, wherein the front space is used for carrying out proliferation dark culture on the germination and branch extraction stages of explants; the middle space is used for carrying out proliferation weak light culture on the explant in a leaf expanding stage; the back space is used for carrying out rooting culture on the proliferated seedlings; the inner wall of the shed frame is provided with a first sunshade net which is positioned in the front space and can be pulled open and a second sunshade net which is positioned in the middle space and can be pulled open, the shading rate of the first sunshade net is controlled to be more than 95%, and the shading rate of the first sunshade net is controlled to be 60% -70%.
From the above description of the present invention, it can be seen that the advantages of the present invention over the prior art are: the seedling culture method is ideal in design, changes the traditional sealwort seedling culture mode, adopts a simple greenhouse to replace a sealwort culture room, namely adopts natural solar illumination as energy, so that not only can energy be saved, but also the sealwort tissue culture seedlings can grow more robustly when receiving sunlight, more importantly, the adaptability of the sealwort tissue culture seedlings to the external environment is enhanced, and later-stage domestication is easy to survive. The cost of seedling culture is low, and large-scale popularization and application can be realized in a short period.
Drawings
Fig. 1 is a schematic side view of a simple greenhouse according to a first embodiment of the present invention.
Fig. 2 is a schematic view of a simple greenhouse in the first embodiment of the present invention.
Fig. 3 is a schematic view of an internal structure of the simple greenhouse of the embodiment.
FIG. 4 is a schematic view of a propagation flask according to the first embodiment.
Fig. 5 is a schematic view of a simple greenhouse in the second embodiment.
Fig. 6 is a schematic side view of the simple greenhouse in the third embodiment.
Fig. 7 is a schematic view of the internal structure of the simple greenhouse in the third embodiment.
Fig. 8 is a schematic top view of the third simple greenhouse of the present embodiment, in which some components are not shown.
Fig. 9 is a schematic view of a seedling production line formed in the third embodiment.
Detailed Description
Example one
A seedling raising method using a simple greenhouse to replace a polygonatum sibiricum culture room comprises the following steps: 1) Selecting and pretreating rhizoma Polygonati, namely selecting underground rhizome of rhizoma Polygonati planted in 9-10 months of the year for 2-3 years, and pretreating the rhizome to serve as an explant; the explant pretreatment comprises the steps of sequentially carrying out cleaning treatment, low-temperature treatment (dormancy breaking of the explant), excess cutting treatment and disinfection treatment on the explant, and specifically comprises the following steps: cleaning underground rhizome (explant) of rhizoma Polygonati with water, removing leaf and root system, cutting off excessive rhizome, removing bract on bud, and collecting middle stem block with bud of about 1.0cm × 1.0cm × 0.5cm; placing the rootstock blocks with buds in an incubator for low-temperature treatment for 4-6 days, controlling the temperature in the incubator at 5-6 ℃, breaking the dormancy of explants, obviously improving the explant induction effect, then washing the rootstock blocks with buds with flowing tap water for 1-2 hours, and airing the rootstock blocks with buds; soaking in 75% ethanol for 15s on a clean bench, washing with sterile water for 2-4 times, adding into 0.1% mercuric chloride, shaking for 8-10min, washing with sterile water for 5-8 times, and drying with sterile filter paper.
2) And (3) proliferation culture: the explant is subpackaged into a proliferation culture bottle 60 (such as a orchid vase with the volume of 500 ml), then the proliferation culture bottle is placed in a simple greenhouse for proliferation culture to obtain a proliferation seedling, the proliferation culture is sequentially subjected to dark culture treatment in the sprouting and branching stage and illumination culture treatment in the leaf unfolding stage of the explant, and the illumination intensity of the illumination culture treatment is controlled to be 3000-5000 lx; the proliferation culture medium comprises: MS +2.0 mg/L6-BA +0.5mg/L NAA + sugar 30g/L, the pH value of the proliferation culture medium is controlled to be 5.5-5.7 (the optimal pH value is 5.6); the proliferation culture medium has the characteristics that: high mortality rate, large leaf area and no green growth of stem and leaf. The temperature in the simple greenhouse is controlled to be about 20-35 ℃. The proliferation culture time can be 3-11 months. After the explants are filled in the proliferation culture bottle 60, the bottle cap is covered, and then the thin film bag 600 is sleeved on the bottle cap, so that the purpose is to prevent external environmental factors from polluting the explants in the bottle body, and simultaneously, a certain heat preservation effect is achieved on the explants in the bottle body. Reference is made in particular to figure 4 of the specification.
3) Rooting culture: and (3) carrying out rooting culture on the proliferated seedlings in a simple greenhouse to obtain rooted seedlings, wherein the illumination intensity in the simple greenhouse is controlled to be 0.5-5 kalx, and the temperature in the simple greenhouse is controlled to be 20-35 ℃. The rooting culture medium comprises: MS +0.5 mg/L6-BA +0.5mg/L NAA + activated carbon 0.3g/L + sugar 30g/L, the pH value of the rooting medium is controlled to 5.5-5.7 (optimum pH value 5.6). The rooting culture medium has the characteristics that: promoting the growth of the rootstocks, and accelerating the growth of the rootstocks and the roots to obtain the seedlings with roots.
4) Treating rooted seedlings; and (4) opening the culture bottle, hardening seedlings for 3 days, taking out, flushing agar by using tap water, and soaking by using 800 times of liquid thiophanate methyl for 15 minutes to obtain the seedling culture medium.
Reference is made to the description accompanying fig. 1, 2 and 3. The simple greenhouse comprises a greenhouse frame 1 and an interlayer 2, wherein the greenhouse frame 1 is formed by sequentially arranging a plurality of steel pipe frameworks 10 at equal intervals, the interlayer 2 divides the greenhouse frame 1 into an upper layer and a lower layer, the lower layer 11 is used for multiplication culture of explants, and the upper layer 12 is used for rooting culture of multiplication seedlings. The interlayer 2 is a light-transmitting partition board with a top sunshade net which can be pulled open, the inner wall of the shed frame 1 is provided with a first sunshade net 31 and a second sunshade net 31 which can be pulled open, and the first sunshade net 31 and the second sunshade net 31 are positioned on the left side wall and the right side wall of the lower layer. The shed frame 1 is also provided with a third sunshade net 32, a fourth sunshade net 32 and a fifth sunshade net 33 which can be pulled open, the left side wall and the right side wall of the upper layer of the third sunshade net 32 and the fourth sunshade net 32 are arranged on the top surface of the shed frame 1 of the fifth sunshade net 33. The shading rates of the third shading net 32, the fourth shading net 32 and the fifth shading net 33 are controlled to be 50-60%; the shading rate of the top shading net and the first shading net 31 and the second shading net 31 is controlled to be 75-95%. A ladder 13 for a worker to get on the upper layer 12 is arranged in the canopy frame 1. The upper layer 12 is provided with an upper culture frame 61 for rooting culture of the proliferated seedlings; the lower layer 11 is provided with a lower culture shelf 62 for propagation culture of explants. The upper and lower cultivation shelves 61, 62 are both provided with an auxiliary light source 7 which can supplement light intensity. The simple greenhouse is preferably arranged on the sunny side to receive more natural light, and the heights of the lower layer 11 and the upper layer 12 are higher than or slightly higher than the heights of the corresponding upper and lower culture racks. The simple greenhouse is adopted to replace the design of the sealwort culture room, namely, natural solar illumination is adopted as energy, so that the energy can be saved, the sealwort tissue culture seedlings can grow more robustly when receiving sunlight, more importantly, the adaptability of the sealwort tissue culture seedlings to the external environment is enhanced, and the sealwort tissue culture seedlings are easy to survive after domestication. Provided that the auxiliary light source 7 is available as a supplement on rainy days. The cost of seedling culture is low, and large-scale popularization and use can be realized in a short period.
Example two
Reference is made to the description accompanying fig. 5. The present embodiment is substantially the same as the first embodiment, except that: according to the consideration of the cost of the simple greenhouse, a heating device for adjusting the temperature in the greenhouse frame 1 can be arranged in the greenhouse frame 1, and the heating device comprises an air conditioner 4 with temperature control (such as an air source heat pump central air conditioner) and an exhaust fan 5.
EXAMPLE III
Refer to the description fig. 6, fig. 7, fig. 8. The present embodiment is substantially the same as the first embodiment, except that: the simple greenhouse has different shapes, the simple greenhouse comprises a greenhouse frame 8, and a first vertical interlayer 91 and a second vertical interlayer 92 which divide the greenhouse frame 8 into a front space 81, a middle space 82 and a rear space 83 in sequence from front to back, wherein the front space 81 is used for carrying out multiplication dark culture on the bud stage and the branch stage of explants; the intermediate space 82 is used for carrying out proliferation and low light culture on the explant in a leaf expanding stage; the back space 83 is used for rooting culture of the proliferated seedlings; the inner wall of the shed frame 8 is provided with a first sunshade net 84 which is positioned in the front space 81 and can be pulled open and a second sunshade net 85 which is positioned in the middle space 82 and can be pulled open, the shading rate of the first sunshade net 84 is controlled to be more than 95%, and the shading rate of the second sunshade net 85 is controlled to be 60% -70%. The simple greenhouse is divided into the front space 81, the middle space 82 and the rear space 83, so that the simple greenhouse can perform regional control and functional region division for different seedling raising stages of the polygonatum sibiricum, control of each spatial region of the simple greenhouse becomes simpler and more targeted, the simple greenhouse enters the corresponding region to perform control according to different seedling raising periods of the polygonatum sibiricum, different functional spatial regions can be conveniently switched along with different seedling raising stages of the polygonatum sibiricum, the illumination and sunshade requirements of the seedling raising period are met, the cost is low, and the energy consumption is low; the temperature and the humidity of the corresponding area in the greenhouse can be adjusted.
Refer to the description fig. 6, 7, 8. The simple greenhouse is provided with an inlet 86 positioned on the front side of the greenhouse frame 8 and an outlet positioned on the rear side of the greenhouse frame 8, and the inlet 86 and the outlet are both provided with a blind 87 or a rolling gate; the ground in the shed frame 8 is provided with a straight track 6 which is arranged from front to back and is matched with the feet of the plurality of culture frames 60, the feet of the plurality of culture frames 60 can be pushed on the straight track 6, and each culture frame 60 can enter from an inlet 86 of the simple greenhouse and then can pass through the front space 81, the middle space 82 and the rear space 83 and then be discharged from an outlet of the simple greenhouse. By adopting the design of the linear track 6, the plurality of culture racks 60 can conveniently enter the corresponding space area to be controlled, the operation of workers is convenient, the seedling raising efficiency is high, and a water-flowing seedling raising production line can be formed.
In addition, reference is made to the description accompanying fig. 9. The simple greenhouse also comprises an inoculation chamber 93 which is positioned on one side of the greenhouse frame 8 and is used for subpackaging explants into propagation culture bottles, a seedling emergence chamber 94 for performing seedling emergence operation on the root seedlings, a culture bottle supply chamber 95 for cleaning, disinfecting and drying the vacant culture bottles, and a preparation chamber 96 for preparing culture medium in the culture bottles; the track is end to end's annular runway formula track 6, rack 8, the room 94 of emerging, blake bottle supply chamber 95, prepare room 96 and inoculation room 93 all connect in series in proper order and form a complete production line of growing seedlings on annular runway formula track. The number of the annular runway type tracks 6 is two, and the two groups of annular runway type tracks are symmetrically arranged left and right by taking the shed frame 8 as a center. By adopting the design of the annular track type track 6, a complete seedling production line can be formed, namely, the preparation of the medium in the culture bottle, the culture of the sealwort in the simple greenhouse, the seedling emergence and the cleaning, disinfecting and drying treatment of the culture bottle are realized to complete a circulating seedling production line.
Example four
A seedling raising method using a simple greenhouse to replace a polygonatum sibiricum culture room comprises the following steps: 1) Selecting and pretreating rhizoma Polygonati, namely selecting underground rhizome of rhizoma Polygonati planted in 9-10 months of the year for 2-3 years, and pretreating the rhizome to serve as an explant; explant pretreatment includes that explant is cleaned, processed at low temperature, cut clout processing and disinfection treatment in proper order, specifically the operation is: cleaning underground rhizome (explant) of rhizoma Polygonati with water, removing leaf and root system, cutting off excessive rhizome, removing bract on bud, and collecting middle stem block with bud of about 1.0cm × 1.0cm × 0.5cm; placing the rhizomes with buds in an incubator for low-temperature treatment for 4-6 days, controlling the temperature in the incubator at 5-6 ℃, then washing the rhizomes with buds with flowing tap water for 1-2 hours, and airing the rhizomes; soaking in 75% ethanol for 15s on a clean bench, washing with sterile water for 2-4 times, adding into 0.1% mercuric chloride, shaking for 8-10min, washing with sterile water for 5-8 times, and drying with sterile filter paper.
2) And (3) inducing the root tuber with the bud: treating the rootstalk blocks with the buds by using a uniform electric field, specifically, placing the rootstalk blocks with the buds into an induction container, and then carrying out induction treatment on the rootstalk blocks with the buds in the induction container by using a 100 kv-300 kv/m uniform electric field (optimally 200 kv/m) emitted by a high-voltage electrostatic generator, wherein the induction treatment time is controlled to be 2-3 minutes; the research shows that: the root tuber with the bud is in a high-voltage electrostatic field, the induction treatment time is within 2-3 minutes, the germination potential, the germination percentage and the dehydrogenase activity of the root tuber with the bud are obviously improved along with the increase of the treatment time, the treatment effect is optimal when the treatment time is 3 minutes, and the treatment effect is gradually weakened along with the continuous increase of the treatment time after the treatment time exceeds 3 minutes.
3) And (3) proliferation culture: subpackaging the explant into a propagation culture bottle (such as a orchid bottle with the volume of 500 ml), placing the propagation culture bottle in a simple greenhouse for propagation culture to obtain a propagation seedling, and sequentially carrying out dark culture treatment in the sprouting and branching stage and illumination culture treatment in the leaf expanding stage of the explant, wherein the illumination intensity of the illumination culture treatment is controlled within 3000-5000lx; the culture medium for proliferating strong seedlings comprises: MS +2.0 mg/L6-BA +0.5mg/L NAA + sugar 30g/L, the pH value of the proliferation culture medium is controlled to be 5.5-5.7 (the optimal pH value is 5.6); the temperature in the simple greenhouse is controlled to be about 20-35 ℃; the relative humidity in the simple greenhouse is preferably kept at 70-80%. The culture time of the proliferation strong seedlings can be 3-11 months.
4) Rooting culture: and (3) carrying out rooting culture on the proliferated seedlings in a simple greenhouse to obtain rooted seedlings, wherein the illumination intensity in the simple greenhouse is controlled to be 0.5-5 kalx, and the temperature in the simple greenhouse is controlled to be 20-35 ℃. The rooting culture medium comprises: MS +0.5 mg/L6-BA +0.5mg/L NAA + 0.3g/L activated carbon + 60g/L sugar, and the pH value of the rooting medium is controlled to 5.5-5.7 (optimum pH value is 5.6).
5) Treating rooted seedlings; uniformly spraying the prepared treatment reagent on the leaves of the rooted seedlings until the leaves drip water, and continuously spraying for 3 days, wherein the treatment reagent is a sodium nitroprusside reagent (namely exogenous NO), and the concentration of the sodium nitroprusside reagent is controlled to be 0.1-0.2 mol.L -1 . The sodium nitroprusside reagent (namely exogenous NO) is adopted to carry out uniform spraying treatment on the leaves of the rooting seedlings, so that the low-temperature stress resistance of the rooting seedlings is improved, the cold-resistant and seedling-strengthening effect is good, the oxidative damage to the rooting seedlings at low temperature can be effectively relieved, the cold resistance of the rooting seedlings is improved, the cultivation of the strong seedlings is promoted, and the adaptability of the rooting seedlings to low temperature is improved.
The above description is only an embodiment of the present invention, but the design concept of the present invention is not limited thereto, and any insubstantial modifications made by using the design concept should fall within the scope of infringing the present invention.
Claims (6)
1. A seedling raising method using a simple greenhouse to replace a polygonatum sibiricum culture room is characterized by comprising the following steps: 1) Selecting and pretreating rhizoma polygonati, namely selecting underground rhizomes of the rhizoma polygonati, and pretreating the underground rhizomes to serve as explants; 2) And (3) proliferation culture: subpackaging the explant into a proliferation culture bottle, then placing the proliferation culture bottle in a simple greenhouse for proliferation culture to obtain a proliferation seedling, wherein the proliferation culture is sequentially subjected to dark culture treatment in a sprouting and branching stage and illumination culture treatment in a leaf unfolding stage of the explant, and the illumination intensity of the illumination culture treatment is controlled to be 3000-5000 lx; 3) Rooting culture: and (3) carrying out rooting culture on the proliferated seedlings in a simple greenhouse to obtain rooted seedlings, wherein the illumination intensity in the simple greenhouse is controlled to be 0.5-5 kalx, and the temperature in the simple greenhouse is controlled to be 20-35 ℃.
2. The seedling raising method using the simple greenhouse to replace a sealwort culture room as claimed in claim 1, which is characterized in that: the simple greenhouse comprises a greenhouse frame and an interlayer which divides the greenhouse frame into an upper layer and a lower layer, wherein the lower layer is used for carrying out enrichment culture on explants, and the upper layer is used for carrying out rooting culture on the enrichment seedlings.
3. The method for growing seedlings by using a simple greenhouse to replace a polygonatum sibiricum culture room according to claim 2, wherein the method comprises the following steps: the interlayer is a light-transmitting partition plate with a first sun-shading net which can be pulled open, the inner wall of the shed frame is provided with a first sun-shading net and a second sun-shading net which can be pulled open, and the first sun-shading net and the second sun-shading net are positioned on the left side wall and the right side wall of the lower layer.
4. The seedling raising method using the simple greenhouse to replace a sealwort culture room as claimed in claim 2, which is characterized in that: the upper layer is provided with an upper culture frame for rooting culture of the proliferated seedlings; the lower layer is provided with a lower culture frame for multiplication culture of explants; and a ladder for a worker to ascend to the upper layer is arranged in the shed frame.
5. The seedling raising method using the simple greenhouse to replace a sealwort culture room as claimed in claim 2, which is characterized in that: and a heating device for adjusting the temperature and humidity in the shed frame is also arranged in the shed frame.
6. The seedling raising method using the simple greenhouse to replace a sealwort culture room as claimed in claim 1, which is characterized in that: the simple greenhouse comprises a greenhouse frame, a first vertical interlayer and a second vertical interlayer, wherein the first vertical interlayer and the second vertical interlayer divide the greenhouse frame into a front space, a middle space and a rear space in sequence from front to back, and the front space is used for carrying out proliferation dark culture on the germination and the branch extraction stage of explants; the middle space is used for carrying out proliferation weak light culture on the explant at a leaf expanding stage; the back space is used for carrying out rooting culture on the proliferated seedlings; the inner wall of the shed frame is provided with a first sunshade net which is positioned in the front space and can be pulled open and a second sunshade net which is positioned in the middle space and can be pulled open, the shading rate of the first sunshade net is controlled to be more than 95%, and the shading rate of the first sunshade net is controlled to be 60% -70%.
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