CN1961654A - Agipanthus tissue cultivation quick-propagation method - Google Patents
Agipanthus tissue cultivation quick-propagation method Download PDFInfo
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- CN1961654A CN1961654A CNA2006101185509A CN200610118550A CN1961654A CN 1961654 A CN1961654 A CN 1961654A CN A2006101185509 A CNA2006101185509 A CN A2006101185509A CN 200610118550 A CN200610118550 A CN 200610118550A CN 1961654 A CN1961654 A CN 1961654A
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- afriocan agapanthus
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- tissue culture
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Abstract
The invention relates to a method for cultivating lotus, which comprises 1, selecting the steam base of lotus as external plant, disinfecting it; 2, seedling, inducing the germ, increment cultivating, differentially cultivating, rooting, and seeding in large house, while the induce culture medium is MS+6-BA 8.0mg/L. The invention has the advantages that: the product has high root rate and high yield.
Description
Technical field
The tissue culture and rapid propagation method of Afriocan agapanthus of the present invention relates to the propagation method of a kind of Afriocan agapanthus, refers to the method for the quick breeding of a kind of Afriocan agapanthus especially.
Background technology
Afriocan agapanthus (Agapanthus africanus) is an Amaryllidaceae, belongs to the perennial showy flowers of herbaceous plants, originates in South Africa, because of its numerous gaining the name of setting seeds after spending, each flower stem of a herb can be blossomed 20 to 50, flower bluish violet, 7～8 months florescences, can do potted plant viewing and admiring, also can plant and locate, grace attractive in appearance in flower bed, garden etc.Afriocan agapanthus natural disposition is strong, and cultivation easily is not tight, more cold-resistant to the soil requirement, is to be adapted at very much the kind that the afforestation aspect is promoted.
Afriocan agapanthus was taken back seed in 2003 by South Africa by the South Africa personage that returns to the homeland, and this kind can be used as ground cover plant and promotes for to introduce domestic garden-variety recently aspect afforestation.But the factor amount is few, and propagation method is mainly plant division routinely, can't breed seedling at short notice in a large number comparatively speaking.
Summary of the invention
The purpose of the tissue culture and rapid propagation method of Afriocan agapanthus of the present invention is to provide a kind of quick breeding and the high Afriocan agapanthus propagation method of survival rate.
For reaching above-mentioned purpose, the invention provides the tissue culture and rapid propagation method of a kind of Afriocan agapanthus, comprise the steps: the first step, choosing Afriocan agapanthus bulb chassis is explant, and explant is carried out aseptic process; Second step, inoculate in regular turn, bud inducing culture, differentiation culture, enrichment culture, culture of rootage, booth hardening, wherein, the bud inducing culture is: MS+6-BA 8.0mg/L.
Described explant is: outer Lao Ye and the root of Afriocan agapanthus are removed, left and taken the part of the long 15cm of lower bottom dribbling cauline leaf.
Described aseptic process is meant that it is the sterilization of 0.05% mercury solution that concentration is put on Afriocan agapanthus bulb chassis, running water washes then, is 1: 3 drift ice solution disinfection again with drift ice and water volume ratio, vacuum drying, move at last that aseptic water washing blots with gauze for 3～4 times on the super-clean bench, stand-by.
The plant that has endophyte for explant will be through 2～3 cultivation cycle and sterilization acquisition once more.
The manual manipulation method of described inoculation is: hand with 75% alcohol disinfecting after, while peelling off blade 75% cotton ball soaked in alcohol wiping hand and explant, to the middle part during 2～3 leaves, to be inoculated on the medium by the cutter cutting-out around the peripheral chassis, a cutter is changed in every partial application, continue again to peel off last blade to the middle part main bud, inoculate behind the top layer around cutting with 75% cotton ball soaked in alcohol wiping hand and explant.
Described sorite proliferated culture medium is: MS+6-BA 4.0mg/L.
Described differential medium is: MS+BA 4.0mg/L.
Described root media: 1/2MS+IBA 0.5mg/L.
Advantage of the present invention is: breed introduced variety Afriocan agapanthus recently fast, and the rooting rate height, hardening survival rate height.
Embodiment
A kind of Afriocan agapanthus tissue culture and rapid propagation method comprises the steps: the first step, and choosing Afriocan agapanthus bulb chassis is explant, and explant is carried out aseptic process; In second step, inoculate in regular turn, bud inducing culture, differentiation culture, enrichment culture, culture of rootage, booth hardening.
One, explant is obtained:
Outer Lao Ye and the root of Afriocan agapanthus are removed, left and taken the part of the long 15cm of lower bottom dribbling cauline leaf.
Two, explant aseptic process:
Described aseptic process is meant that it is the sterilization of 0.05% mercury solution that concentration is put on Afriocan agapanthus bulb chassis, running water washes then, is 1: 3 drift ice solution disinfection again with drift ice and water volume ratio, vacuum drying, move at last that aseptic water washing blots with gauze for 3～4 times on the super-clean bench, stand-by.
The plant that has endophyte for explant will be through 2～3 cultivation cycle and sterilization acquisition once more.
Three, explant inoculation
Hand with 75% alcohol disinfecting after, while peelling off blade 75% cotton ball soaked in alcohol wiping hand and explant, to the middle part during 2～3 leaves, to be inoculated on the medium by the cutter cutting-out around the peripheral chassis, a cutter is changed in every partial application, continue again to peel off last blade to the middle part main bud, inoculate behind the top layer around cutting with 75% cotton ball soaked in alcohol wiping hand and explant.Wherein, the bud inducing culture is: MS+6-BA 8.0mg/L begins to form callus and has budlet to generate after 45 days.
Four, differentiation and enrichment culture
Selecting MS+BA 4.0mg/L is differential medium, and differentiation culture formed from bud in 1～2 month, reduced hormonal readiness gradually, cultivated in proliferated culture medium MS+6-BA 4.0mg/L, and its breeding ratio is 1: 3, reaches the proper proportion of fast numerous production.
Five, culture of rootage
Selecting medium 1/2MS+IBA 2.0mg/L is root media, sprouts 18 days root time, rooting rate 100%.
Six, booth hardening
The new root eruption in Afriocan agapanthus field planting booth 1 week back of taking root, survival rate 100%.
Claims (8)
1, the tissue culture and rapid propagation method of a kind of Afriocan agapanthus comprises the steps: the first step, and choosing Afriocan agapanthus bulb chassis is explant, and explant is carried out aseptic process; Second step, inoculate in regular turn, bud inducing culture, differentiation and enrichment culture, culture of rootage, booth hardening, wherein, the bud inducing culture is: MS+6-BA8.0mg/L.
2, the tissue culture and rapid propagation method of Afriocan agapanthus according to claim 1 is characterized in that described explant is: outer Lao Ye and the root of Afriocan agapanthus are removed, left and taken the part of the long 15cm of lower bottom dribbling cauline leaf.
3, the tissue culture and rapid propagation method of Afriocan agapanthus according to claim 1 and 2, it is characterized in that: described aseptic process is meant that it is the sterilization of 0.05% mercury solution that concentration is put on Afriocan agapanthus bulb chassis, running water flushing then, be 1: 3 drift ice solution disinfection again with drift ice and water volume ratio, vacuum drying, move at last that aseptic water washing blots with gauze for 3～4 times on the super-clean bench, stand-by.
4, the tissue culture and rapid propagation method of Afriocan agapanthus according to claim 3 is characterized in that: the plant that has endophyte for explant will be through 2～3 cultivation cycle and sterilization acquisition once more.
5, the tissue culture and rapid propagation method of Afriocan agapanthus according to claim 1 and 2, it is characterized in that: the manual manipulation method of described inoculation is: hand with 75% alcohol disinfecting after, while peelling off blade 75% cotton ball soaked in alcohol wiping hand and explant, to the middle part during 2～3 leaves, to be inoculated on the medium by the cutter cutting-out around the peripheral chassis, a cutter is changed in every partial application, continue again with 75% cotton ball soaked in alcohol wiping hand and explant, peel off last blade to the middle part main bud, inoculate behind the top layer around cutting.
6, the tissue culture and rapid propagation method of Afriocan agapanthus according to claim 1 and 2 is characterized in that described differential medium is: MS+BA4.0mg/L.
7, the tissue culture and rapid propagation method of Afriocan agapanthus according to claim 1 and 2 is characterized in that described sorite proliferated culture medium is: MS+6-BA4.0mg/L.
8, the tissue culture and rapid propagation method of Afriocan agapanthus according to claim 1 and 2 is characterized in that described root media: 1/2MS+IBA0.5mg/L.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2006101185509A CN1961654A (en) | 2006-11-21 | 2006-11-21 | Agipanthus tissue cultivation quick-propagation method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2006101185509A CN1961654A (en) | 2006-11-21 | 2006-11-21 | Agipanthus tissue cultivation quick-propagation method |
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| CN1961654A true CN1961654A (en) | 2007-05-16 |
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| CNA2006101185509A Pending CN1961654A (en) | 2006-11-21 | 2006-11-21 | Agipanthus tissue cultivation quick-propagation method |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101869066B (en) * | 2009-04-24 | 2012-01-04 | 上海上房园林植物研究所 | Tissue culture method of African agapanthus |
| CN102823582A (en) * | 2012-09-18 | 2012-12-19 | 上海交通大学 | Vitrification ultralow-temperature preserving method for agapanthus embryogenic callus |
| CN103947548A (en) * | 2014-04-11 | 2014-07-30 | 宁波城市职业技术学院 | Method for establishing agapanthus high-frequency regeneration system |
| CN108293862A (en) * | 2018-01-25 | 2018-07-20 | 上海为绿景观建设有限公司 | A kind of rapid propagation method of Afric lilium |
| CN109220791A (en) * | 2018-09-12 | 2019-01-18 | 上海市农业科学院 | A kind of tissue culture method using bulb breeding Hipeastrum vittalum (L Her.) Herb.- Amaryllisvittata Ait |
| CN109937883A (en) * | 2019-04-26 | 2019-06-28 | 上海市园林科学规划研究院 | The seedling fast breeding method of the short raw double variety ' diamond weight ' of Afriocan agapanthus |
| CN116649221A (en) * | 2023-07-26 | 2023-08-29 | 云南昊辰农业有限公司 | Tissue culture and rapid propagation method for agapanthus |
-
2006
- 2006-11-21 CN CNA2006101185509A patent/CN1961654A/en active Pending
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101869066B (en) * | 2009-04-24 | 2012-01-04 | 上海上房园林植物研究所 | Tissue culture method of African agapanthus |
| CN102823582A (en) * | 2012-09-18 | 2012-12-19 | 上海交通大学 | Vitrification ultralow-temperature preserving method for agapanthus embryogenic callus |
| CN103947548A (en) * | 2014-04-11 | 2014-07-30 | 宁波城市职业技术学院 | Method for establishing agapanthus high-frequency regeneration system |
| CN108293862A (en) * | 2018-01-25 | 2018-07-20 | 上海为绿景观建设有限公司 | A kind of rapid propagation method of Afric lilium |
| CN109220791A (en) * | 2018-09-12 | 2019-01-18 | 上海市农业科学院 | A kind of tissue culture method using bulb breeding Hipeastrum vittalum (L Her.) Herb.- Amaryllisvittata Ait |
| CN109220791B (en) * | 2018-09-12 | 2021-10-08 | 上海市农业科学院 | Tissue culture method for breeding hippeastrum rutilum by using bulbs |
| CN109937883A (en) * | 2019-04-26 | 2019-06-28 | 上海市园林科学规划研究院 | The seedling fast breeding method of the short raw double variety ' diamond weight ' of Afriocan agapanthus |
| CN116649221A (en) * | 2023-07-26 | 2023-08-29 | 云南昊辰农业有限公司 | Tissue culture and rapid propagation method for agapanthus |
| CN116649221B (en) * | 2023-07-26 | 2023-09-22 | 云南昊辰农业有限公司 | Tissue culture and rapid propagation method for agapanthus |
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