CN115250872A - Planting organic matrix containing probiotics as well as preparation method and application thereof - Google Patents
Planting organic matrix containing probiotics as well as preparation method and application thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
- A01G24/23—Wood, e.g. wood chips or sawdust
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The invention discloses a planting organic matrix containing probiotics as well as a preparation method and application thereof. The organic matrix for planting comprises the following raw materials in parts by weight: 32 to 40 parts of straw, 20 to 30 parts of sawdust, 20 to 25 parts of mushroom residue, 0.5 to 5 parts of probiotic and 5 to 10 parts of traditional Chinese medicine composition; the traditional Chinese medicine composition comprises the following raw materials in parts by weight: 3 to 5 parts of lateral bur leaves, 2 to 4 parts of common cnidium fruit, 1 to 3 parts of radix stemonae and 3 to 6 parts of amur corktree bark. The lactobacillus plantarum TH103 and the lactobacillus rhamnosus zrx are used in a combined mode, so that the drug effect absorption and the drug effect of a traditional Chinese medicine formula can be enhanced, meanwhile, the traditional Chinese medicine composition can promote the growth of probiotics, and the probiotics further promote the growth of plants, so that the traditional Chinese medicine composition has the very remarkable effects of promoting the growth of plants, resisting diseases and insect pests, improving the yield and improving the quality of crops.
Description
Technical Field
The invention relates to the field of plant cultivation, in particular to a planting organic matrix containing probiotics and a preparation method and application thereof.
Background
The agricultural industry develops rapidly to generate a large amount of agricultural waste resources, such as straws, waste fungus sticks, sawdust and the like. The agricultural waste resource is a good available resource, and is fully utilized to improve the resource utilization efficiency and the economic added value, so that the resource pressure in the economic development process of China is relieved, the environmental pollution is reduced, and the virtuous circle sustainable development of agricultural production is realized. Meanwhile, as a large amount of fertilizers and pesticides are used in agriculture, not only is the land environment damaged, but also the food safety problem is caused, so that the health of people is harmed, and people increasingly recognize the importance of producing pollution-free green organic plants. The traditional Chinese medicine not only contains amino acids, saccharides, organic matters, crude protein and other various nutrient elements required by plants, but also contains various factors such as sterilization, insect inhibition and the like, and the fertilizer produced by the traditional Chinese medicine meets the requirements of people on the safety of the fertilizer and pesticide.
At present, the nursery stock and organic fruits and vegetables planted by soil have high cost and great difficulty, and are difficult to put an end to diseases and pests in the soil, heavy metal pesticide residues in the soil and the like, so the fruits and vegetables produced by the soil are difficult to reach the organic standard, while the seedling raising matrix is mostly composed of naturally formed turf, vermiculite, perlite and the like, so the problem of no disease and pest propagation can not be fundamentally solved, the matrix does not contain beneficial bacteria, the soil-borne diseases are eliminated during soil planting, pesticide spraying is still needed for preventing and treating, the soil environment is affected, food safety exists, and in order to solve the problem, the invention takes crop wastes as the base material, screens out the traditional Chinese medicine composition with the functions of bacteriostasis and disinsection and the probiotic capable of promoting the efficacy of traditional Chinese medicine components, and develops an organic matrix for planting capable of promoting plant growth, resisting diseases and pests, improving yield and improving crop quality.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provides a planting organic matrix containing probiotics as well as a preparation method and application thereof so as to solve the problems in the technical background.
Chinese patent: lactobacillus plantarum TH103 and application thereof (application number 201510385613.6, applied to the university of Hebei engineering) disclose lactobacillus plantarum TH103, which is preserved in the China general microbiological culture Collection center (CGMCC), with the preservation date: year 2015, 4, 23; the preservation number is CGMCC10739. The lactobacillus plantarum TH103 used in the present invention was obtained from researchers of north-Hebei engineering university.
Chinese patent: lactobacillus rhamnosus and application thereof in preparing bacterin (application number is 201810181018.4; application is Henan science and technology institute) disclose Lactobacillus rhamnosus zrx, wherein the strain is preserved in the China general microbiological culture Collection center; the preservation date is as follows: 12 months and 22 days 2017; the preservation number is CGMCC No.15076. The lactobacillus rhamnosus zrx used in the invention is given by researchers of the science and technology institute of Henan.
In the research and practice processes of the team, the invention discovers that the traditional Chinese medicine composition with antibacterial and insecticidal effects is used as a base material for fermentation, the probiotics jointly use lactobacillus plantarum TH103 and lactobacillus rhamnosus zrx01, the prepared organic matrix for planting can effectively promote plant growth, enhance disease and pest resistance, improve yield and improve crop quality, the organic matrix for planting prepared by singly using lactobacillus plantarum TH103, lactobacillus rhamnosus zrx or other strains to be matched with the traditional Chinese medicine composition with the antibacterial and insecticidal effects or the organic matrix for planting prepared by singly jointly using probiotics and not adding the traditional Chinese medicine composition has far better effect than the organic matrix for planting prepared by jointly using lactobacillus plantarum TH103 and lactobacillus rhamnosus zrx to be matched with the traditional Chinese medicine composition for fermentation, the effect of the lactobacillus plantarum TH103 and lactobacillus rhamnosus zrx can be enhanced, the traditional Chinese medicine composition can promote plant growth, the microbial inoculum can further promote plant growth, and has very obvious effects of promoting plant growth, enhancing the yield of plants, improving plant quality and improving crop quality.
In order to achieve the purpose, the invention is realized by the following technical scheme:
in a first aspect, the invention provides a planting organic matrix containing probiotics, which comprises the following raw materials in parts by weight: 32 to 40 parts of straw, 20 to 30 parts of sawdust, 20 to 25 parts of mushroom residue, 0.5 to 5 parts of probiotic and 5 to 10 parts of traditional Chinese medicine composition; the traditional Chinese medicine composition comprises the following raw materials in parts by weight: 3 to 5 parts of lateral bur leaves, 2 to 4 parts of common cnidium fruit, 1 to 3 parts of radix stemonae and 3 to 6 parts of amur corktree bark.
In the technical scheme, the organic matrix containing probiotics for planting comprises the following raw materials in parts by weight: 36 parts of straw, 25 parts of sawdust, 22 parts of mushroom residue, 2 parts of probiotic agent and 8 parts of traditional Chinese medicine composition.
In the technical scheme, the probiotic agent comprises lactobacillus plantarum TH103 fermented filtrate and lactobacillus rhamnosus zrx fermented filtrate.
In the technical scheme, the volume ratio of the lactobacillus plantarum TH103 fermentation filtrate to the lactobacillus rhamnosus zrx fermentation filtrate is 1:1; the concentration of the lactobacillus plantarum TH103 in the lactobacillus plantarum TH103 fermentation filtrate is 10 7 ～10 8 CFU/ml; the concentration of the lactobacillus rhamnosus zrx in the fermentation filtrate of the lactobacillus rhamnosus zrx is 10 7 ～10 8 CFU/ml。
In a second aspect, the present invention provides a method for preparing the organic substrate for planting containing probiotics, comprising the following steps:
(1) Preparing materials: compounding the formulation of the organic planting matrix of claim 2;
(2) Treating the traditional Chinese medicine composition: extracting folium Platycladi, fructus Cnidii, radix Stemonae, and cortex Phellodendri with water to obtain Chinese medicinal extract, and collecting Chinese medicinal residue;
(3) Adding probiotic agent into the Chinese medicine extract obtained in the step (2) to obtain fermentation raw material, and fermenting the fermentation raw material at 32-37 ℃ for 2-3 days to obtain fermented product;
(4) Adding straws, mushroom residues and the fermentation product obtained in the step (3) into the traditional Chinese medicine filter residues obtained in the step (2), uniformly mixing, stacking into a stack, adding water, keeping the initial water content at 56-60%, fermenting and decomposing for 20-40 d to obtain a decomposed organic fertilizer;
(5) And mixing the decomposed organic fertilizer and the sawdust in proportion to obtain the organic matrix.
In the technical scheme, in the step (4), the materials piled up in a pile are covered with straws, and the straws are lifted and piled up once every 48 hours.
In a third aspect, the invention provides the use of the organic planting matrix containing probiotics for planting crops.
One of the innovations of the invention is that the probiotics jointly use lactobacillus plantarum TH103 and lactobacillus rhamnosus zrx, so that a synergistic effect is achieved on the growth of plants;
the second innovation of the invention is that the traditional Chinese medicine composition with insecticidal effect is used as a base material for fermentation, the lactobacillus plantarum TH103 and lactobacillus rhamnosus zrx are jointly used by the probiotics, the prepared organic matrix for planting can effectively promote plant growth, enhance insecticidal effect and improve peanut quality, the lactobacillus plantarum TH103 and lactobacillus rhamnosus zrx are jointly used to enhance drug effect absorption and drug effect of effective components in the traditional Chinese medicine composition, and meanwhile, the traditional Chinese medicine composition can promote the growth of the probiotics and further promote plant growth, so that the invention has very remarkable effect.
Compared with the prior art, the invention has the beneficial effects that:
1. through research and practice of the inventor, the probiotics jointly use lactobacillus plantarum TH103 and lactobacillus rhamnosus zrx which are compatible with straws, sawdust and mushroom residues, and can promote plants to absorb effective ingredients in the straws, the sawdust and the mushroom residues, so that a synergistic effect is achieved for promoting plant growth;
2. according to the application, the probiotic is adopted to ferment the traditional Chinese medicine composition, on one hand, the effect of the pesticide effect of the sterilization and deinsectization components in the traditional Chinese medicine composition can be further enhanced through fermentation, and then the traditional Chinese medicine composition has a promoting effect on the growth of the probiotic, so that the traditional Chinese medicine composition can improve the activity of a strain and promote the growth of the probiotic, the effect of fermentation can be improved, and further the nutrient components in the organic matrix can be easily absorbed by plants and can be promoted to grow.
3. The lactobacillus plantarum TH103 and the lactobacillus rhamnosus zrx are used together, so that the drug effect absorption and the drug effect of the traditional Chinese medicine formula can be enhanced, meanwhile, the traditional Chinese medicine composition can promote the growth of probiotics and the probiotics further promote the growth of plants, and the effects of remarkably promoting the growth of plants, resisting diseases and insect pests, increasing the yield and improving the quality of crops are achieved.
Drawings
FIG. 1 is a bar graph showing the effect of different organic substrates on the emergence time and height of alfalfa in test example 1.
Detailed Description
The embodiments of the present invention are described below with reference to specific embodiments, and other advantages and effects of the present invention will be easily understood by those skilled in the art from the disclosure of the present specification. The invention is capable of other and different embodiments and of being practiced or of being carried out in various ways, and its several details are capable of modification in various respects, all without departing from the spirit and scope of the present invention. It is to be noted that the features in the following embodiments and examples may be combined with each other without conflict.
Example 1
The embodiment provides a planting organic matrix containing probiotics, which comprises the following raw materials in parts by weight: 32 parts of straw, 30 parts of sawdust, 25 parts of mushroom residue and 0.5 part of probiotic10 parts of a traditional Chinese medicine composition; wherein the straw is corn straw, wheat straw or rice straw and the like; the mushroom residue is waste mushroom residue after planting edible mushroom, such as mushroom residue; the traditional Chinese medicine composition comprises the following raw materials in parts by weight: 3 parts of lateral bur leaves, 4 parts of common cnidium fruit, 3 parts of radix stemonae and 3 parts of amur corktree bark. The probiotic agent comprises lactobacillus plantarum TH103 fermentation filtrate and lactobacillus rhamnosus zrx fermentation filtrate. The volume ratio of the lactobacillus plantarum TH103 fermentation filtrate to the lactobacillus rhamnosus zrx fermentation filtrate is 1:1; the concentration of the lactobacillus plantarum TH103 in the lactobacillus plantarum TH103 fermentation filtrate is 10 7 CFU/ml; the concentration of the lactobacillus rhamnosus zrx in the fermentation filtrate of the lactobacillus rhamnosus zrx is 10 8 CFU/ml. The lactobacillus plantarum TH103 fermentation filtrate is lactobacillus plantarum TH103 activated culture according to a conventional method, and the concentration of a bacterial liquid is adjusted to 10 by a culture medium 7 cfu/ml. The lactobacillus rhamnosus zrx fermentation filtrate is activated and cultured by the conventional method.
Example 2
The embodiment provides a planting organic matrix containing probiotics, which comprises the following raw materials in parts by weight: 36 parts of straw, 25 parts of sawdust, 22 parts of mushroom residue, 2 parts of probiotic and 8 parts of traditional Chinese medicine composition. The traditional Chinese medicine composition comprises the following raw materials in parts by weight: 4 parts of lateral bur leaves, 3 parts of common cnidium fruit, 2 parts of radix stemonae and 5 parts of amur corktree bark. The probiotic agent comprises lactobacillus plantarum TH103 fermentation filtrate and lactobacillus rhamnosus zrx fermentation filtrate. The volume ratio of the lactobacillus plantarum TH103 fermentation filtrate to the lactobacillus rhamnosus zrx fermentation filtrate is 1:1; the concentration of the lactobacillus plantarum TH103 in the lactobacillus plantarum TH103 fermentation filtrate is 10 8 CFU/ml; the concentration of the lactobacillus rhamnosus zrx in the fermentation filtrate of the lactobacillus rhamnosus zrx is 10 8 CFU/ml。
Example 3
The embodiment provides a planting organic matrix containing probiotics, which comprises the following raw materials in parts by weight: 40 parts of straw, 20 parts of sawdust, 20 parts of mushroom residue, 5 parts of probiotic and 5 parts of traditional Chinese medicine composition; the traditional Chinese medicine composition comprises the following raw materials in parts by weight: 5 parts of lateral bur leaves, 2 parts of common cnidium fruit, 1 part of sessile stemona root and 6 parts of amur corktree bark. The probiotic agent comprises lactobacillus plantarum TH103 fermentation filtrate and lactobacillus rhamnosus zrx fermentation filtrate. The volume ratio of the lactobacillus plantarum TH103 fermentation filtrate to the lactobacillus rhamnosus zrx fermentation filtrate is 1:1; the concentration of the lactobacillus plantarum TH103 in the lactobacillus plantarum TH103 fermentation filtrate is 10 8 CFU/ml; the concentration of the lactobacillus rhamnosus zrx in the fermentation filtrate of the lactobacillus rhamnosus zrx is 10 7 CFU/ml。
Comparative example 1
This comparative example is similar to example 2, except that no traditional Chinese medicine composition is included. Specifically, the organic planting matrix containing probiotics comprises the following raw materials in parts by weight: 36 parts of straw, 25 parts of sawdust, 22 parts of mushroom residue and 2 parts of probiotic; the probiotic agent comprises lactobacillus plantarum TH103 fermentation filtrate and lactobacillus rhamnosus zrx fermentation filtrate.
Comparative example 2
This comparative example is similar to example 2, except that the probiotic is different. Specifically, the organic planting matrix containing probiotics comprises the following raw materials in parts by weight: 36 parts of straw, 25 parts of sawdust, 22 parts of mushroom residue, 2 parts of probiotic and 8 parts of traditional Chinese medicine composition. The traditional Chinese medicine composition comprises the following raw materials in parts by weight: 4 parts of Chinese arborvitae twig, 3 parts of common cnidium fruit, 2 parts of radix stemonae and 5 parts of amur corktree bark. The probiotic agent is lactobacillus plantarum TH103 fermentation filtrate.
Comparative example 3
This comparative example is similar to example 2, except that the probiotic is different. Specifically, the organic planting matrix containing probiotics comprises the following raw materials in parts by weight: 36 parts of straw, 25 parts of sawdust, 22 parts of mushroom residue, 2 parts of probiotic agent and 8 parts of traditional Chinese medicine composition. The traditional Chinese medicine composition comprises the following raw materials in parts by weight: 4 parts of Chinese arborvitae twig, 3 parts of common cnidium fruit, 2 parts of radix stemonae and 5 parts of amur corktree bark. The probiotic agent is lactobacillus rhamnosus zrx fermentation filtrate.
Comparative example 4
This comparative example is similar to example 2, except that the probiotic is different. Specifically, the organic planting matrix containing probiotics comprises the following raw materials in parts by weight: 36 parts of straw, 25 parts of sawdust, 22 parts of mushroom residue, 2 parts of probiotic agent and 8 parts of traditional Chinese medicine composition. The traditional Chinese medicine composition comprises the following raw materials in parts by weight: 4 parts of lateral bur leaves, 3 parts of common cnidium fruit, 2 parts of radix stemonae and 5 parts of amur corktree bark. The probiotic agent is lactobacillus plantarum TH103 fermentation filtrate and common lactobacillus rhamnosus fermentation filtrate. The lactobacillus rhamnosus is purchased from China general microbiological culture Collection center with the preservation number of CGMCC 1.120.
Comparative example 5
This comparative example is similar to example 2, except that no probiotic was included. Specifically, the organic planting matrix containing probiotics comprises the following raw materials in parts by weight: 36 parts of straw, 25 parts of sawdust, 22 parts of mushroom residue and 8 parts of traditional Chinese medicine composition. The traditional Chinese medicine composition comprises the following raw materials in parts by weight: 4 parts of lateral bur leaves, 3 parts of common cnidium fruit, 2 parts of radix stemonae and 5 parts of amur corktree bark.
Comparative example 6
This comparative example is similar to comparative example 1, except that the probiotic was different. Specifically, the organic planting matrix containing probiotics comprises the following raw materials in parts by weight: 36 parts of straw, 25 parts of sawdust, 22 parts of mushroom residue and 2 parts of probiotic. The probiotic agent is lactobacillus plantarum TH103 fermentation filtrate.
Comparative example 7
This comparative example is similar to comparative example 1, except that the probiotic was different. Specifically, the organic planting matrix containing probiotics comprises the following raw materials in parts by weight: 36 parts of straw, 25 parts of sawdust, 22 parts of mushroom residue and 2 parts of probiotic. The probiotic agent is lactobacillus rhamnosus zrx fermentation filtrate.
Comparative example 8
This comparative example is similar to comparative example 1, except that the probiotic was different. Specifically, the organic planting matrix containing probiotics comprises the following raw materials in parts by weight: 36 parts of straw, 25 parts of sawdust, 22 parts of mushroom residue and 2 parts of probiotic. The probiotic agent is lactobacillus plantarum TH103 fermentation filtrate and common lactobacillus rhamnosus fermentation filtrate. The lactobacillus rhamnosus is purchased from China general microbiological culture Collection center with the preservation number of CGMCC 1.120.
Comparative example 9
This comparative example is similar to comparative example 1, except that no probiotic was included. Specifically, the organic planting matrix containing probiotics comprises the following raw materials in parts by weight: 36 parts of straw, 25 parts of sawdust and 22 parts of mushroom residue.
Examples 1-3 were all prepared as follows:
the preparation method of the organic matrix containing probiotics for planting comprises the following steps:
(1) Preparing materials: preparing materials according to the formula of the corresponding organic matrix for planting;
(2) Treating the traditional Chinese medicine composition: extracting folium Platycladi, fructus Cnidii, radix Stemonae, and cortex Phellodendri with water to obtain Chinese medicinal extract, and collecting Chinese medicinal residue;
wherein, the water extraction specifically comprises: weighing the medicinal materials according to the formula, crushing the medicinal materials into powder, adding water into the obtained powder, decocting for 3 times, combining decoction, evaporating and concentrating at 95-100 ℃ to 1/8-1/10 of the original volume, and thus obtaining the traditional Chinese medicine extract. Specifically, the traditional Chinese medicine extract is prepared by weighing the medicinal materials according to the composition of the traditional Chinese medicine composition, crushing the medicinal materials into traditional Chinese medicine powder, adding water with the amount which is 6-8 times of the total weight of the medicinal materials, soaking for 15-30 min, decocting for 1-1.5 h, filtering, collecting filtrate, adding water with the same amount of the medicinal residues into the medicinal residues, decocting for 1h, filtering, collecting filtrate, repeating the steps once, combining the three filtrates, and evaporating and concentrating at 100 ℃ to 1/8-1/10 of the original volume.
(3) Adding probiotic agent into the Chinese medicine extract obtained in the step (2) to obtain fermentation raw material, and fermenting the fermentation raw material at 32-37 ℃ for 2-3 days to obtain fermented product;
(4) Adding straws, mushroom residues and the fermentation product obtained in the step (3) into the traditional Chinese medicine filter residues obtained in the step (2), uniformly mixing, stacking into a stack, adding water, keeping the initial water content at 56-60%, fermenting and decomposing for 20-40 d to obtain a decomposed organic fertilizer; wherein, covering the piled materials with straws, and lifting the straws once every 48 hours and turning the straws for 4 to 5 times.
(5) Mixing the decomposed organic fertilizer and sawdust (sawdust of 10-40 meshes) in proportion to obtain the organic matrix.
Comparative examples 1-9 were prepared according to the above method, and when no probiotic was included, the maturation fermentation time was increased by 1-3 months.
Test example 1 Effect of organic substrate on plant growth
The organic substrates obtained in examples 1-3 and comparative examples 1-9 are used as test soil, alfalfa seeds are used for pot culture tests, 13 groups are arranged in total, wherein 1 group is a control group, and the control group adopts soil commonly used for pot culture; 12 treatment groups were prepared by using the organic substrates obtained in examples 1 to 3 and comparative examples 1 to 9, respectively; each group is provided with 10 repetitions, and each repetition is provided with 20 alfalfa pots; the growing period of the potted plant is observed, and the time of emergence, the rate of emergence, the trefoil stage and the height of the seedling are counted, and the results are shown in table 1 and figure 1.
TABLE 1 Effect of different organic substrates on growth period of potted plants
Note: a: p <0.05 compared to control; b: p <0.05 compared to any of comparative examples 1-5; c: p <0.05 compared to any of comparative examples 6-9;
as can be seen from table 1 and fig. 1, in example 2, compared with comparative examples 1 to 5, the effect of emergence time, emergence rate, trefoil stage and seedling height in comparative examples 1 to 5 is significantly lower than that in example 2, which indicates that the effect of planting organic substrate prepared by combining lactobacillus plantarum TH103, lactobacillus rhamnosus zrx or other strains with a traditional Chinese medicine composition having antibacterial and insecticidal efficacy, or planting organic substrate prepared by combining lactobacillus plantarum TH103 and lactobacillus rhamnosus zrx alone without adding a traditional Chinese medicine composition is far less than that of planting organic substrate prepared by combining lactobacillus plantarum TH103 and lactobacillus rhamnosus 3242 with a traditional Chinese medicine composition for fermentation, and indicates that the combined use of lactobacillus plantarum TH103 and lactobacillus rhamnosus zrx can enhance the absorption and efficacy of traditional Chinese medicine formula, and the traditional Chinese medicine composition can promote the growth of probiotics and further promote the growth of plants, thus having very significant effects of promoting the growth of plants, resisting diseases, increasing yield and improving crop quality.
Compared with comparative examples 6-9, the comparative example 1 shows that the effects of emergence time, emergence rate, trefoil stage and seedling height in the comparative examples 6-9 are obviously lower than those in the comparative example 1, and that the probiotics jointly uses lactobacillus plantarum TH103 and lactobacillus rhamnosus zrx which are compatible with straw, sawdust and mushroom residues and can promote plants to absorb effective ingredients in the straw, the sawdust and the mushroom residues, so that the synergistic effect on promoting plant growth is achieved.
Test example 2 Effect of organic base on tomato planting
In the same greenhouse, the organic matrixes obtained in examples 1-3 and comparative examples 1-9 are used as test soil to plant tomatoes, 13 groups are arranged in total, wherein 1 group is a control group, the control group 1 adopts greenhouse planting common soil, and the control group 2 respectively adopts the organic matrixes obtained in examples 1-3 and comparative examples 1-9; each group is designed with 6 replicates, the same variety is cultivated, the daily management measures are the same, the disease and pest morbidity, the yield and the quality (the content of soluble total sugar, the content of lycopene and VC) of the tomatoes are statistically analyzed, and the results are shown in table 2.
Wherein, the incidence rate of diseases and insect pests (%) = number of diseases/total number of surveys × 100; the content of soluble total sugar is measured by a sulfuric acid-anthrone method phenol method; the lycopene is measured by using an ultraviolet-visible spectrophotometer; the VC content is measured by iodometry.
TABLE 2 influence of organic matrix on tomato pest, yield, quality
Note: a: p <0.05 compared to control; b: p <0.05 compared to any of comparative examples 1-5; c: p <0.05 compared to any of comparative examples 1, 6-9;
as can be seen from table 2, compared with the control group and the comparative examples 1 to 5, the incidence of plant diseases and insect pests is significantly reduced, and the yield and the tomato quality are significantly improved in the examples 1 to 3, which indicates that the organic substrate for planting prepared by using lactobacillus plantarum TH103, lactobacillus rhamnosus zrx or other strains in combination to prepare a traditional Chinese medicine composition with antibacterial and insecticidal effects, or using probiotic alone in combination to prepare an organic substrate for planting prepared without adding a traditional Chinese medicine composition, has far better effect than using lactobacillus plantarum TH103 and lactobacillus rhamnosus zrx in combination to prepare an organic substrate for planting by fermenting traditional Chinese medicine compositions, and indicates that the combination of lactobacillus plantarum TH103 and lactobacillus rhamnosus zrx can enhance the drug effect absorption and drug effect of the traditional Chinese medicine composition, and the traditional Chinese medicine composition can promote the growth of probiotic and the probiotic to further promote the plant growth, thereby having very significant effects of promoting the plant growth, resisting plant diseases and insect pests, improving the yield and improving the crop quality.
Test example 3 testing the effect of the ingredients of the Chinese medicinal materials on the probiotic
The applicant selects a plurality of traditional Chinese medicines with bacteriostatic and insecticidal effects common in the prior art, and tests the influence of the traditional Chinese medicines on the probiotics, wherein the selected traditional Chinese medicine extract comprises Chinese arborvitae twig, common cnidium fruit, sessile stemona root, amur corktree bark, lightyellow sophora root, rhubarb, mulberry leaf and hairyvein agrimony;
wherein, the extracts are all extracted by water, and the water extraction method comprises the following steps: respectively pulverizing the raw materials (folium Potentillae Discoloris, fructus Cnidii, radix Stemonae, cortex Phellodendri, radix Sophorae Flavescentis, radix et rhizoma Rhei, folium Mori or herba et Gemma Agrimoniae) into powder, decocting the obtained powder with water for 3 times according to the material-to-liquid ratio of 1:8, mixing decoctions, evaporating and concentrating at 95-100 deg.C to 1/10 of the original volume, and cooling to room temperature to obtain water extract of each raw material.
Respectively replacing the water extracts with the traditional Chinese medicine composition in the steps (2) to (3) in the preparation method of the organic substrate containing the probiotics for planting, namely adding probiotics (according to the proportion of the traditional Chinese medicine composition and the probiotics in the embodiment 2) into the water extracts (the water extracts of the Chinese medicinal materials of the lateral bur leaves, the common cnidium fruit, the sessile stemona root, the amur corktree bark, the lightyellow sophora root, the rhubarb, the mulberry leaves or the hairyvein agrimonia herb and bud) of the raw material medicines, wherein the probiotics comprise lactobacillus plantarum TH103 fermentation filtrate and lactobacillus rhamnosuszrx01 fermentation filtrate. The volume ratio of the lactobacillus plantarum TH103 fermentation filtrate to the lactobacillus rhamnosus zrx fermentation filtrate is 1:1; the concentration of the lactobacillus plantarum TH103 in the lactobacillus plantarum TH103 fermentation filtrate is 10 8 CFU/ml; the concentration of the lactobacillus rhamnosus zrx in the fermentation filtrate of the lactobacillus rhamnosus zrx is 10 8 CFU/ml. Obtaining fermentation raw materials, and fermenting the fermentation raw materials for 2-3 days at the temperature of 32-37 ℃ to obtain a fermented product; the effective viable count of the probiotic in the initial fermentation raw material and the fermented product obtained by fermentation is tested to judge the promotion or inhibition effect of the water extract of the lateral bur leaves, the common cnidium fruit, the sessile stemona root, the amur corktree bark, the lightyellow sophora root, the rhubarb, the mulberry leaves or the hairyvein agrimony on the growth of the probiotic, and the specific value is shown in table 3.
TABLE 3 influence of different Chinese medicinal water extracts on the effective viable count of probiotic
As can be seen from Table 3, the water extracts of folium Araliae chinensis, fructus Cnidii, radix Stemonae and cortex Phellodendri all can increase the effective viable count of probiotic in the fermented product, while the water extracts of radix Sophorae Flavescentis, radix et rhizoma Rhei, folium Mori and herba et Gemma Agrimoniae are added to increase the effective viable count of probiotic in the fermented product, but decrease.
By combining the tests, the Chinese medicinal composition is fermented by the probiotic, the effective ingredients in the Chinese medicinal composition are effectively decomposed and utilized, the disease resistance of crops planted by using the organic matrix is enhanced, and the purpose of reducing the use of chemical pesticides can be achieved.
The above embodiments only express specific embodiments of the present invention, and the description is specific and detailed, but not to be construed as limiting the scope of the present invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention.
Claims (7)
1. The organic planting matrix containing probiotics is characterized by comprising the following raw materials in parts by weight: 32 to 40 parts of straw, 20 to 30 parts of sawdust, 20 to 25 parts of mushroom residue, 0.5 to 5 parts of probiotic and 5 to 10 parts of traditional Chinese medicine composition; the traditional Chinese medicine composition comprises the following raw materials in parts by weight: 3 to 5 parts of lateral bur leaves, 2 to 4 parts of common cnidium fruit, 1 to 3 parts of radix stemonae and 3 to 6 parts of amur corktree bark.
2. The planting organic matrix containing probiotics as claimed in claim 1, which comprises the following raw materials by weight: 36 parts of straw, 25 parts of sawdust, 22 parts of mushroom residue, 2 parts of probiotic and 8 parts of traditional Chinese medicine composition.
3. The organic planting substrate containing probiotics as claimed in claim 1, wherein the probiotics comprise lactobacillus plantarum TH103 fermentation filtrate and lactobacillus rhamnosus zrx fermentation filtrate.
4. The organic planting substrate containing probiotics as claimed in claim 3, wherein the volume ratio of the Lactobacillus plantarum TH103 fermented filtrate to the Lactobacillus rhamnosus zrx fermented filtrate is 1:1; the concentration of the lactobacillus plantarum TH103 in the lactobacillus plantarum TH103 fermentation filtrate is 10 7 ～10 8 CFU/ml; the concentration of the lactobacillus rhamnosus zrx in the fermentation filtrate of the lactobacillus rhamnosus zrx is 10 7 ～10 8 CFU/ml。
5. The method for preparing organic planting substrate containing probiotics according to claim 3, comprising the following steps:
(1) Preparing materials: compounding the formulation of the organic planting matrix of claim 3;
(2) Treating the traditional Chinese medicine composition: extracting folium Platycladi, fructus Cnidii, radix Stemonae, and cortex Phellodendri with water to obtain Chinese medicinal extract, and collecting Chinese medicinal residue;
(3) Adding probiotic agent into the Chinese medicine extract obtained in the step (2) to obtain fermentation raw material, and fermenting the fermentation raw material at 32-37 ℃ for 2-3 days to obtain fermented product;
(4) Adding straws, mushroom residues and the fermentation product obtained in the step (3) into the traditional Chinese medicine filter residues obtained in the step (2), uniformly mixing, stacking into a stack, adding water, keeping the initial water content at 56-60%, fermenting and decomposing for 20-40 d to obtain a decomposed organic fertilizer;
(5) Mixing the decomposed organic fertilizer and the sawdust in proportion to obtain the organic matrix.
6. A method as claimed in claim 5, wherein in step (4) the material is topped on the pile and the straw is lifted and dumped once every 48 hours.
7. Use of a planting organic matrix containing probiotics according to any one of claims 1-4 for crop planting.
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