CN115227617A - Skin repairing composition and preparation method and application thereof - Google Patents
Skin repairing composition and preparation method and application thereof Download PDFInfo
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- CN115227617A CN115227617A CN202210950495.9A CN202210950495A CN115227617A CN 115227617 A CN115227617 A CN 115227617A CN 202210950495 A CN202210950495 A CN 202210950495A CN 115227617 A CN115227617 A CN 115227617A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/005—Antimicrobial preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/52—Stabilizers
- A61K2800/522—Antioxidants; Radical scavengers
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
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- Organic Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Cosmetics (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a skin repairing composition and a preparation method and application thereof, and relates to the technical field of cosmetics. The repairing plant composition consists of centella and schisandra, the extract is prepared in an oil extraction mode, and the obtained extract contains abundant madecassoside and schizandrin and has excellent bacteriostatic, anti-inflammatory and free radical removing effects.
Description
Technical Field
The invention relates to the technical field of cosmetics, and particularly relates to a skin repairing composition as well as a preparation method and application thereof.
Background
In the environment in which people live, various factors can affect the condition of the skin. Such as living stress, environmental pollution, changes in four seasons, use of chemicals, etc., may cause damage to the skin and skin mucosa, resulting in an increasing incidence of skin diseases. In addition, the dysbacteriosis and the breeding of pathogenic bacteria of a human body can cause endocrine dysfunction, sebum exuberance often appears on the skin after reaction, and meanwhile, inflammation, acne redness, pimple on the top and the like are caused. When the epidermal flora of a human body is disordered, the breeding of pathogenic bacteria can cause inflammatory reaction of the skin, and meanwhile, the generated inflammatory factors can stimulate the skin in turn, so that the increase of active oxygen free radicals is caused, more damage is generated, and the phenomena of acne redness, epidermal water loss and the like occur. Inhibiting the growth of harmful bacteria can reduce the risk of skin infection. COX-2 mediates the reaction of pain, inflammation and fever by promoting PG synthesis, and reduces prostaglandin synthesis by inhibiting COX, thereby having antiinflammatory effect. Meanwhile, the inflammation reaction can be weakened by reducing active oxygen free radicals, clearing inflammatory factors, improving stratum corneum water loss and the like. Based on this, there is an increasing demand for cosmetics having repair effects such as antibacterial and anti-inflammatory effects.
Chinese patent application CN201711306417.0 discloses a cosmetic composition of centella asiatica extract with anti-inflammatory and antioxidant complex functions, the formula comprises the following components by weight percent: 5-10% of centella asiatica extract; 1-5% of poncirus trifoliata extract; 1-5% of giant knotweed extract; 2-6% of chamomile extract; 3-7% of sophora flavescens extract; 0.5-2% of green tea extract; fructus Schisandrae extract 0.5-2%; the balance is cosmetic matrix. The invention adopts a flash extraction method to extract the centella, greatly improves the extraction efficiency of active ingredients in the centella, has good operability and little pollution, reduces the production cost, can effectively extract triterpenoids in the centella, furthest retains other active ingredients in the centella, and is matched with the active ingredients in chamomile and poncirus trifoliata to be organically combined with green tea extract, giant knotweed rhizome extract, lightyellow sophora root extract and Chinese magnoliavine fruit extract with high-efficiency antioxidant effect so as to achieve high-efficiency anti-inflammatory antioxidant composite effect.
Chinese patent application CN201710747402.1 discloses a cassia twig volatile oil with antibacterial and anti-inflammatory activities, and a preparation method and application thereof, wherein the preparation method comprises the following steps: 1) Sterilizing the ramulus Cinnamomi ethanol extract residue at 121 deg.C for 30min, cooling, and taking out; 2) Adding the sterilized and cooled cassia twig alcohol extract residue into the fermentation bacterium suspension under the ventilation and aerobic conditions, uniformly mixing, fermenting, and collecting a fermentation product; 3) Taking out the fermented product after fermentation, drying and crushing to obtain fermented cassia twig alcohol extract residue powder. 4) Adding 5 times of organic solvent by weight into the fermented cassia twig alcohol extract residue powder, shaking uniformly, soaking for 3 days at room temperature and in a dark place (20-25 ℃), filtering, and recovering the organic solvent by a rotary film decompression method to obtain the cassia twig volatile oil. The product has antibacterial, antiinflammatory, antioxidant, and microcirculation improving effects.
At present, fewer cosmetics with good and definite repair efficacy need to be developed, repair cosmetics with remarkable efficacy need to be developed, and the existing repair cosmetics have more components, adopt various repair components, and increase cost and extraction complexity. In view of this, the present invention provides a skin-repairing composition, a method for preparing the same and applications thereof.
Disclosure of Invention
The invention aims to provide a skin repairing and caring composition, a preparation method and application thereof, wherein the repairing and caring plant composition is composed of centella and schisandra chinensis, an extract is prepared in an oil extraction mode, and the obtained extract contains abundant madecassoside and schizandrin and has excellent bacteriostatic, anti-inflammatory and free radical removing effects.
In order to achieve the purpose, the technical scheme of the invention is as follows:
in one aspect, the present invention provides a botanical composition comprising centella asiatica and schisandra chinensis.
Preferably, the mass ratio of the centella asiatica to the schisandra chinensis is 1-3:1-3.
Further preferably, the mass ratio of the centella asiatica to the schisandra chinensis is 3:1.
in still another aspect, the present invention provides a method for extracting the plant composition, comprising the steps of:
mixing herba Centellae, fructus Schisandrae chinensis and extraction solvent, extracting, and separating to obtain filtrate.
Preferably, the extraction solvent is at least two of lecithin, caprylic/capric triglyceride, and jojoba oil; further preferably a mixed solvent of lecithin and caprylic/capric triglyceride, or a mixed solvent of lecithin and jojoba oil; furthermore, the extraction solvent is lecithin and caprylic/capric triglyceride in a mass ratio of 1:3-5, or the mass ratio of lecithin to jojoba oil is 1:3-5 of a mixed solvent; further, the extraction solvent is lecithin and caprylic/capric triglyceride in a mass ratio of 1:4, or the mass ratio of the lecithin to the jojoba oil is 1:4 in a mixed solvent; most preferably, the mass ratio of lecithin to jojoba oil is 1:4 in a solvent mixture.
Preferably, the ratio of the extracted feed liquid to the extracted feed liquid is 1:15 to 50 (m/m), more preferably 1:50 (m/m).
The term "m/m" represents a mass ratio.
Preferably, the extraction time is 1-3h, more preferably 2h.
Preferably, the temperature of the extraction is 50 to 150 ℃, more preferably 90 ℃.
In still another aspect, the present invention provides a plant extract obtained according to the above extraction method.
In a further aspect, the present invention provides the use of the above plant composition and/or plant extract for the preparation of a cosmetic having a repairing effect.
Preferably, the repair efficacy includes scavenging free radicals, bacteriostasis and anti-inflammation.
Finally, the present invention provides a cosmetic with a repairing efficacy comprising the above plant composition and/or plant extract.
The beneficial effects of the invention are as follows:
the cosmetic raw materials prepared by the preparation method are safe and environment-friendly, have natural components, no toxic or side effect and definite repair effect, and have excellent bacteriostatic, anti-inflammatory and free radical removing effects through experimental verification. The extraction method is simple, the extraction efficiency of the effective components is high, and the components are simple and safe.
Detailed Description
The present invention will be further explained with reference to specific examples in order to make the technical means, the technical features, the technical objectives and the effects of the present invention easier to understand, but the following examples are only preferred embodiments of the present invention, and not all embodiments of the present invention. Based on the embodiments in the implementation, other embodiments obtained by those skilled in the art without any creative efforts belong to the protection scope of the present invention. In the following examples, unless otherwise specified, all the operations were performed by conventional methods, all the equipments were performed by conventional methods, and the materials of the equipments used in the respective examples were the same. All concentration percentages are mass concentrations unless otherwise specified. If not stated otherwise, in the experiment of the present invention, when the plant composition extraction filtrate is prepared into a solution with a specific concentration, all the solvents used are caprylic/capric triglyceride.
In the embodiment described below, it is preferred that,
lecithin was purchased from Hebei America vitamin technology Inc. under the designation LLE/20210605.
Caprylic/capric triglyceride is available from Shandong Yousio chemical science and technology, inc. under the product number 099195003100.
Jojoba oil was purchased from kininojie constant science trade ltd, beijing.
Centella asiatica is a Chinese medicinal material sale limited company in Hebei Deyi, and the production place is Sichuan.
Schisandra chinensis is Beijing Tongrentang, and its origin is Changbai mountain in northeast China.
Example 1
The weight ratio of the asiatic pennywort herb to the Chinese magnoliavine fruit is as follows: 1:1.
Example 2
The weight ratio of the asiatic pennywort herb to the Chinese magnoliavine fruit is as follows: 2:1.
Example 3
The weight ratio of the asiatic pennywort herb to the Chinese magnoliavine fruit is as follows: 3:1.
Example 4
The weight ratio of the asiatic pennywort herb to the Chinese magnoliavine fruit is as follows: 1:2.
Example 5
The weight ratio of the asiatic pennywort herb to the Chinese magnoliavine fruit is as follows: 1:3.
The extraction method comprises the following steps:
the compositions of examples 1-5 were all extracted in the following manner:
weighing 2g of each mixed medicinal material in the embodiments 1-5 respectively, adding 20g of lecithin and 80g of caprylic/capric triglyceride, stirring uniformly, and extracting in water bath at 90 ℃ for 2h. Cooling the material liquid to 25 deg.C after extraction, and filtering with 200 mesh gauze to obtain extractive solution.
Example 6
As in example 3, 32g caprylic/capric triglyceride was replaced with 32g jojoba oil at the time of extraction.
Result detection
1. Content detection
The detection method comprises the following steps: sodium nitrite-aluminum nitrate-sodium hydroxide colorimetric method
And (3) detection results:
in the extract, the average value of the total flavone content is 0.62-0.97mg/mL, which proves that the extraction efficiency of the extraction method of the invention is high for centella asiatica and schisandra chinensis, wherein, as can be seen from example 3, when the weight ratio of centella asiatica to schisandra chinensis is 3.
2. Experiment for inhibiting bacteria
The experimental method comprises the following steps: in a sterile 96-well culture plate, 100. Mu.L of diluted bacterial suspension was added to each well of the test wells, 100. Mu.L of extract solutions of different concentrations (10%, 5%) were added, 100. Mu.L of samples of different concentrations + 100. Mu.L of LB broth were used as a control, 200. Mu.L of LB broth without P.acnes was used as a blank control, 100. Mu.L of diluted bacterial suspension + 100. Mu.L of LB broth was used as a negative control, 100. Mu.L of diluted bacterial suspension + 100. Mu.L, and 200. Mu.g/mL of sodium penicillin were used as positive controls, and 3 replicate wells were made, respectively.
Sterile 96-well culture plates were placed in a 37 ℃ incubator. After 12h of incubation, the absorbance was measured at 600 nm. After plating, the plate was placed in an incubator and cultured for 12 hours, and then the OD value at 600nm was measured.
Inhibition ratio:
the results of propionibacterium acnes inhibition were as follows:
the results of inhibition of staphylococcus aureus were as follows:
the results of the inhibition rate of pseudomonas aeruginosa were as follows:
the results show that the extracts of examples 2 and 6 had good Propionibacterium acnes-inhibiting effect at a concentration of 5%, and that the extracts of examples 1 to 6 had excellent Propionibacterium acnes-inhibiting effect at a concentration of 10%. The extract of example 6 showed a good inhibitory effect against Staphylococcus aureus at a concentration of 5%, and the extracts of examples 1 to 6 showed a good inhibitory effect against Staphylococcus aureus at a concentration of 10%. Examples 3 and 5 showed good Pseudomonas aeruginosa inhibitory effect at a concentration of 5%, and examples 1 to 3 showed good Pseudomonas aeruginosa inhibitory effect at a concentration of 10%.
3. COX-2 inhibition assay
The experimental method comprises the following steps: according to a COX-2 kit (Shanghai Biyuntian biotechnology limited, S0168), the COX-2 inhibition rate of 3.00% in different sample concentrations is tested, and the detection is carried out according to the operation instruction of the kit.
The results are as follows:
the results showed that example 6 had the best inhibitory effect, and examples 2 and 5 had the better inhibitory effect on COX-2.
4. DPPH radical scavenging
The experimental method comprises the following steps: taking the extracting solution to prepare solutions to be detected with the concentrations of 5.0%, 10%, 15% and 20% respectively. The DPPH solution is prepared by using absolute ethyl alcohol as a solvent for later use. Tube A: uniformly mixing 1mL of a solution to be detected with 1mL of 2X 10-4mol/L DPPH solution; and (B) tube: uniformly mixing 1mL of solvent with 1mL of 2X 10-4mol/L DPPH solution; c, pipe C: uniformly mixing 1mL of solvent with 1mL of solution to be detected; after each tube was reacted for 30min in the dark, absorbance values of tubes A, B and C were measured at 517 nm.
DPPH free radical inhibition rate calculation formula: DPPH inhibition ratio (%) = (B + C-A)/B
DPPH free radical inhibition results were as follows:
the results show that examples 1 and 5 have high DPPH free radical inhibition rate and good antioxidant effect at a concentration of 10%, and examples 1 to 5 have good DPPH free radical inhibition effect at a concentration of 20%.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (10)
1. A plant composition is characterized by comprising centella asiatica and schisandra chinensis.
2. The botanical composition according to claim 1, wherein the mass ratio of centella asiatica to schisandra chinensis is 1-3:1-3.
3. The botanical composition according to claim 1, wherein the mass ratio of centella asiatica to schisandra chinensis is 3:1.
4. a method for extracting a plant composition as claimed in any one of claims 1 to 3, comprising the steps of: mixing the centella asiatica, the schisandra chinensis and an extraction solvent according to the formula dosage, extracting, and separating to obtain a filtrate, wherein the extraction solvent is a mixed solvent of lecithin and caprylic/capric triglyceride or a mixed solvent of lecithin and jojoba oil.
5. The extraction method according to claim 4, wherein the extraction solvent is a mixture of lecithin and caprylic/capric triglyceride in a mass ratio of 1:3-5, or the mass ratio of lecithin to jojoba oil is 1: 3-5.
6. The extraction method according to claim 5, wherein the extraction solvent is lecithin and caprylic/capric triglyceride in a mass ratio of 1:4, or the mass ratio of the lecithin to the jojoba oil is 1:4 in a mixed solvent; preferably the mass ratio of lecithin to caprylic/capric triglyceride is 1:4 in a solvent mixture.
7. The extraction method according to claim 4, wherein the ratio of the extracted feed to the liquid is 1:15-50 (m/m), the extraction time is 1-3h, and the extraction temperature is 50-150 ℃.
8. Plant extracts obtained by the extraction process according to any one of claims 1 to 7.
9. Use of the plant composition of any one of claims 1 to 3 and/or the plant extract of claim 10 for the preparation of a cosmetic product with a rejuvenating effect comprising scavenging free radicals, bacteriostatic action and anti-inflammatory action.
10. A cosmetic with a repairing efficacy, comprising the plant composition according to any one of claims 1 to 3 and/or the plant extract according to claim 8.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210950495.9A CN115227617A (en) | 2022-08-09 | 2022-08-09 | Skin repairing composition and preparation method and application thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210950495.9A CN115227617A (en) | 2022-08-09 | 2022-08-09 | Skin repairing composition and preparation method and application thereof |
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| CN115227617A true CN115227617A (en) | 2022-10-25 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202210950495.9A Pending CN115227617A (en) | 2022-08-09 | 2022-08-09 | Skin repairing composition and preparation method and application thereof |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107582458A (en) * | 2017-10-11 | 2018-01-16 | 广州勇达生物科技有限公司 | A kind of sunscreen composition and preparation method thereof |
| CN108096089A (en) * | 2017-12-11 | 2018-06-01 | 百朗德生物化学(海门)有限公司 | A kind of cosmetic composition of Gotu Kola P.E with anti-inflammatory anti-oxidant complex function and its preparation method and application |
| WO2019122738A1 (en) * | 2017-12-22 | 2019-06-27 | Basf Beauty Care Solutions France Sas | Use of guerbet alcohol and/or caprylic/capric triglyceride as extraction solvent |
| CN111803411A (en) * | 2020-06-04 | 2020-10-23 | 黑龙江澳利达奈德制药有限公司 | Skin care product containing schisandra extract and preparation method thereof |
-
2022
- 2022-08-09 CN CN202210950495.9A patent/CN115227617A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107582458A (en) * | 2017-10-11 | 2018-01-16 | 广州勇达生物科技有限公司 | A kind of sunscreen composition and preparation method thereof |
| CN108096089A (en) * | 2017-12-11 | 2018-06-01 | 百朗德生物化学(海门)有限公司 | A kind of cosmetic composition of Gotu Kola P.E with anti-inflammatory anti-oxidant complex function and its preparation method and application |
| WO2019122738A1 (en) * | 2017-12-22 | 2019-06-27 | Basf Beauty Care Solutions France Sas | Use of guerbet alcohol and/or caprylic/capric triglyceride as extraction solvent |
| CN111803411A (en) * | 2020-06-04 | 2020-10-23 | 黑龙江澳利达奈德制药有限公司 | Skin care product containing schisandra extract and preparation method thereof |
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