CN103585277A - Application of clove leaf extractives in inhibition of streptococcus suis and intervention and elimination of streptococcus suis biofilm - Google Patents
Application of clove leaf extractives in inhibition of streptococcus suis and intervention and elimination of streptococcus suis biofilm Download PDFInfo
- Publication number
- CN103585277A CN103585277A CN201310486414.5A CN201310486414A CN103585277A CN 103585277 A CN103585277 A CN 103585277A CN 201310486414 A CN201310486414 A CN 201310486414A CN 103585277 A CN103585277 A CN 103585277A
- Authority
- CN
- China
- Prior art keywords
- streptococcus suis
- biofilm
- folium caryophylli
- extract
- folium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Landscapes
- Medicines Containing Plant Substances (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
Abstract
The invention discloses an application of clove leaf extractives in inhibition of streptococcus suis and intervention and elimination of a streptococcus suis biofilm. The intervention effect of the clove leaf extractives to the formation of the streptococcus suis biofilm and the elimination effect of the clove leaf extractives to the mature streptococcus suis biofilm are researched by using a crystal violet method and a scanning electron microscope. An experiment result proves that the clove leaf extractives have a remarkable intervention effect on the formation of the streptococcus suis biofilm and a remarkable elimination effect for the mature streptococcus suis biofilm, so that the clove leaf extractives can be used for inhibiting the adhesion of the streptococcus suis and the formation of the streptococcus suis biofilm and can take the elimination effect on the mature biofilm. The invention provides a novel treating approach for relevant infections caused by the streptococcus suis biofilm.
Description
Technical field
The present invention relates to the pharmacological use that Folium Caryophylli extract is new, relate in particular to Folium Caryophylli extract suppressing Streptococcus suis and intervene Streptococcus suis biofilm to form, eliminate the purposes in the ripe biofilm of Streptococcus suis, belong to the pharmacological use field of Folium Caryophylli extract.
Background technology
Streptococcus suis (Streptococcus suis, S.suis) be a kind of important zoonosis pathogen, can infected pigs cause meningitis, pneumonia, arthritis, septicemia etc., its pathogenicity is strong, case fatality rate is high, drug resistance is strong, is the important pathogenic bacteria that causes pig morbidity.Due to the extensive use of broad ectrum antibiotic, S.suis declining, causes S.suis infection rate to be ascendant trend year by year to nearly all antibiotic sensitivity in recent years.The resistance mechanism of S.suis comprises many aspects, be exactly wherein to form one deck biofilm (Biofilm, BF) on its surface, BF is that antibacterial generation polycomplex substrate holds self, adhere to non-activity object or live body surface, the bacterial community that has a fixed structure of formation; It can resist host immune power and antibiotic effect, and in tunicle, antibacterial easily produces drug resistance widely to antibiotic, causes to infect to be difficult to cure outbreak repeatedly.
Bacterial biofilm is because single or multiple types antibacterial is in order to adapt to surrounding, be adsorbed in foreign body or tissue surface, breed and secrete the polysaccharide protein complexs such as a large amount of polysaccharide matrixes, fibrin, lipoprotein, make antibacterial inter-adhesive, assemble, form the film spline structure with 3-D solid structure, be Bacterial microcolony aggregation.The formation of biofilm comprises that formation, the differentiation of initially sticking stage, surperficial microcolony become ripe 3 processes.Cause infecting generation repeatedly, be difficult to control, once biofilm forms, in film, antibacterial increases more than 100～1000 times than planktonic bacteria drug resistance.Biofilm stops and suppresses macrophage, leukocyte, antibody and antibiotic and invade kill bacteria in biofilm, causes infecting repeatedly occurring, and is difficult to control.
At present both at home and abroad result of study shows 14, fifteen-membered ring Macrocyclolactone lactone kind medicine has the effect of intervening or eliminating S.suis biofilm, but Chinese medicine is to the intervention of S.suis biofilm or the rarely seen report of elimination effect.
Modern pharmacological research proof Folium Caryophylli has the effects such as heat-clearing and toxic substances removing, antibiotic, antiinflammatory, antiviral, antioxidation, eliminating phlegm and relieving cough.But Folium Caryophylli has the research of inhibition or elimination effect aspect to there is not yet report to Streptococcus suis biofilm.
Summary of the invention
Main purpose of the present invention is to provide the new pharmacological use of Folium Caryophylli extract.
The present invention sets up Streptococcus suis (S.suis) Biofilm model first in vitro, observes the effect of Folium Caryophylli water extract to S.suis biofilm.The research with elimination effect to the intervention of Streptococcus suis biofilm by Folium Caryophylli extract, the infections relating causing for Streptococcus suis biofilm provides new treatment approach.
The present invention by crystal violet method (CV) and scanning electron microscope method evaluation Folium Caryophylli extract to the intervention of Streptococcus suis biofilm and elimination effect and the Morphology Effects to Streptococcus suis biofilm.Under scanning electron microscope, observe and show that Folium Caryophylli extract has obvious intervention and elimination effect to show that Folium Caryophylli extract can suppress sticking of Streptococcus suis in 1/2MIC concentration to Streptococcus suis biofilm, intervene the formation of Streptococcus suis biofilm and ripe biofilm is had to elimination effect.
In addition, the present invention's discovery, Folium Caryophylli extract has the inhibitory action of highly significant for Streptococcus suis, therefore, Folium Caryophylli extract can be prepared into the medicine that suppresses Streptococcus suis.
The invention provides a kind of pharmaceutical composition that suppresses Streptococcus suis, this pharmaceutical composition comprises: the Folium Caryophylli extract of effective dose and pharmaceutically acceptable adjuvant and carrier.
The present invention also provides a kind of pharmaceutical composition that suppresses the growth of Streptococcus suis biofilm, and this medicine comprises: the Folium Caryophylli extract of effective dose and pharmaceutically acceptable adjuvant and carrier.
The present invention further provides a kind of pharmaceutical composition of eliminating Streptococcus suis biofilm, and this pharmaceutical composition comprises: the Folium Caryophylli extract of effective dose and pharmaceutically acceptable adjuvant and carrier.
Folium Caryophylli extract described in the present invention can be Folium Caryophylli water alcohol mixed extract, Folium Caryophylli water extract or Folium Caryophylli alcohol extract.The preparation method of Folium Caryophylli water alcohol mixed extract, Folium Caryophylli water extract or Folium Caryophylli alcohol extract is open in the literature, according to the resulting Folium Caryophylli extract of disclosed various extracting method in document (comprising water alcohol mixed extraction method, alcohol extracting method or water extraction), all can be applicable to the present invention.
As a reference, the preparation method of a kind of Folium Caryophylli water alcohol mixed extract of the present invention, comprising: Folium Caryophylli is extracted after soaking with Diluted Alcohol after pulverizing, and extracting solution is concentrated, obtains; Described Diluted Alcohol is 20-50% ethanol, is preferably 40% ethanol; 60min is extracted in described being extracted as at 65 ℃ of temperature.
The research of relevant Folium Caryophylli pharmacological action shows, Folium Caryophylli biological activity is higher, and action effect is lasting.At present the antibiotic that adopt are intervened and eliminate Streptococcus suis biofilm more, and not only toxic and side effects greatly but also easily make the drawback of microorganisms drug resistance; Adopt Folium Caryophylli extract can effectively intervene the formation of Streptococcus suis biofilm or eliminate Streptococcus suis biofilm, do not have toxic and side effects, antibacterial is difficult for producing drug resistance, has antibiotic and antiviral persistency; Folium Caryophylli is the wide plant of a class distributed pole, and aboundresources is used safety, so Folium Caryophylli extract is applied to be prepared into the medicine of intervening or eliminating Streptococcus suis biofilm, application prospect is clinically very optimistic.
Accompanying drawing explanation
The intervention effect experimental result of Fig. 1 Folium Caryophylli extract 1/2MIC to Streptococcus suis (S.suis) biofilm.
The elimination effect experimental result of Fig. 2 Folium Caryophylli extract 1/2MIC to Streptococcus suis (S.suis) biofilm.
The specific embodiment
By the following example, embodiments of the present invention will be more specifically described, it should be understood that described embodiment is only exemplary, does not form any restriction to scope of the present invention.It will be understood by those skilled in the art that lower without departing from the spirit and scope of the present invention and can the details of technical solution of the present invention and form be modified or be replaced, but these modifications or replacement all fall into protection scope of the present invention.
Experiment material
1 bacterial strain: Streptococcus suis (S.suis) (separated strain) is provided by Harbin Veterinary Medicine Inst., China Academy of Agriculture.
2 medicines: Folium Caryophylli (in the school, inventor plucks voluntarily in Northeast Agricultural University), azithromycin.
3 culture medium: THB culture medium.
4 reagent: pH7.4 phosphate buffered solution, crystal violet, methanol, glacial acetic acid, calf serum, FeCl
3.
5 materials: Calgary biomembrane device, 96 porocyte culture plates, 0.22 μ m filter membrane.
The preparation of embodiment 1 Folium Caryophylli extract
Measure Folium Caryophylli 50g, after pulverizing, add 40% ethanol 500mL, greenhouse soaks one hour, 65 ℃ are extracted 3h, pour out medicinal liquid, 12 layers of filtered through gauze, add again 40% ethanol 300mL65 ℃ to extract 60min, pour out medicinal liquid equally by 12 layers of filtered through gauze, merge medicinal liquid and be concentrated into finite concentration, filtrate is through freeze dryer lyophilizing.Precision weighing freeze-dried powder 1g, uses 4mL deionized water dissolving, centrifuging and taking supernatant, with 0.22 μ m membrane filtration, be kept at 4 ℃ standby.
Experimental example 1 Folium Caryophylli extract is to the intervention of Streptococcus suis (S.suis) and elimination effect experiment
1 test method
The mensuration of 1.1 Folium Caryophylli extracts to the minimum inhibitory concentration of S.suis (MIC)
The standard micro-dilution method that test operation and result judgement are recommended according to national standard body of U.S. clinical laboratory (NCCLS) is measured Folium Caryophylli extract to the minimum inhibitory concentration of S.suis (MIC).
Pig streptococcus bacterial strain is inoculated in to THB agar plate, 37 ℃ of overnight incubation.Get single colony inoculation in THB culture medium, cultivate 12h～16h for 37 ℃.Turbidimetry is first adjusted into 0.5 Maxwell pipe suspension (1.0 * 10 by bacterial concentration
8cfumL
-1), with the dilution of THB culture medium, be then approximately 1.0 * 10
6cfumL
-1.To aseptic 96 porocyte culture plates, add THB culture medium successively, except the 1st hole adds 160 μ L THB culture medium, all the other every pipes add 100 μ L THB, in the 1st hole, add Folium Caryophylli extracting solution 40 μ L to mix, then draw 100 μ L to the 2 holes, after mixing, draw again 100 μ L to the 3 holes, so continuously doubling dilution to the 11 holes, and from the 11st hole, draw 100 μ L and discard, the 12nd hole is not containing the growth control of medicine.Then in every hole, add each 100 μ L of the above-mentioned S.suis bacterium liquid preparing, make the final bacterial concentration of every pipe be about 5 * 10
5cFU/ml.The 1st hole to the 11 hole drug level are respectively 25mg/ml, 12.5mg/ml, 6.25mg/ml, 3.125mg/ml, 1.5625mg/ml, 0.78125mg/ml etc.After mixing, build, in 37 ℃, hatch 24h.With perusal, the medicine least concentration hole without bacterial growth, is the MIC of Folium Caryophylli extract to S.suis.
1.2S.suis Biofilm model is set up
Pig streptococcus bacterial strain is inoculated in to THB agar plate, 37 ℃ of overnight incubation.Get single colony inoculation in THB culture medium, cultivate 12h～16h for 37 ℃.By the bacterium liquid dilution of turbidity Wei0.5 Maxwell unit, be approximately 1.0 * 10
6cfumL
-1, get 200 μ L and be inoculated in the biofilm device of Calgary, only the hole containing THB culture medium is matched group, after mixing, builds, and 37 ℃, on 50rmp shaking table, hatches 72h.Take out Calgary biofilm device, with liquid-transfering gun discard culture medium and with the PBS(pH7.2 of sterilizing) wash gently three times to remove the bacterium that dissociates.Still the tunicle being attached on the biofilm device of Calgary is fixed to 30 minutes with 200 μ L methanol.Discard fixative after natural air drying, under room temperature by the crystal violet of 200 μ L0.1% to the biofilm 30min that dyes.Dye complete, discard dyeing liquor, with PBS(pH7.2) clean twice to remove free dyeing liquor, air-dry, then every hole adds the glacial acetic acid solution that 200 μ L concentration are 33%, is placed in agitator 10min to discharge the crystal violet of biofilm.Microplate reader 595nm place measures OD value.
The formation ability criterion of Streptococcus suis biofilm: the meansigma methods of marginal value (ODc)=negative control+(standard deviation of 3 * negative control).When OD≤ODc, judge that antibacterial inanimate object tunicle forms ability; As ODc < OD≤2 * OD
ctime, a little less than judging that bacterial biofilm formation ability is; As 2 * OD
c< OD≤4 * OD
ctime, judge that bacterial biofilm forms ability medium; As 4 * OD
cduring < OD, judge that bacterial biofilm forms ability strong.
The intervention effect of 1.31/2MIC to S.suis biofilm
By the S.suis bacterium liquid dilution of turbidity Wei0.5 Maxwell unit, be approximately 1.0 * 10
6cfumL
-1to adding bacterium liquid and Folium Caryophylli extract totally 200 μ l(concentration 1/2MIC in the every hole of biofilm device, Calgary), separately establish blank hole (only adding culture medium), negative control hole (the not biofilm growth control of dosing) and positive control hole (azithromycin of concentration 1/2MIC); Azithromycin has good inhibitory action to the formation of Streptococcus suis biofilm, therefore this experiment adopts azithromycin as positive control, validating experiment method is correctly feasible.Five repetitions of each sample.37 ℃, on 50rmp shaking table, hatch 72h, PBS cleans 2 times, fixing, and OD is surveyed in dyeing
595value.
The elimination effect of 1.41/2MIC to S.suis biofilm
By the S.suis bacterium liquid dilution of turbidity Wei0.5 Maxwell unit, be approximately 1.0 * 10
6cfumL
-1get 200 μ L and be inoculated in 96 orifice plates, 37 ℃, on 50rmp shaking table, hatch 72h, PBS cleans 2 times, in the every hole of biomembrane device, Calgary, add THB culture medium and Folium Caryophylli extract 200 μ l(1/2MIC), continue at 37 ℃, on 50rmp shaking table, hatch 72h, PBS cleans 3 times, fixing, dyeing, surveys OD
595value.Separately establish blank hole (only adding culture medium), negative control hole (the not biofilm growth control of dosing) and positive control hole (azithromycin of 1/2MIC).
The morphological observation of 1.5 Folium Caryophylli extracts to the effect of S.suis biofilm
First from Calgary biofilm device, take off the dowel pin that forms biofilm, and use sterilizing PBS(pH7.2) wash gently three times to remove free bacterium, with pH7.2 glutaraldehyde, fix, and be placed in 4 ℃ of refrigerators and fix 1 hour.With 0.1mol pH7.2 phosphate buffer, rinse twice successively, be 10 minutes at every turn, use again 50%, 70%, 90% dewatering of ethanol respectively once, each 10～15 minutes, then use 100% ethanol dehydration secondary, each 10～15 minutes, last 100% ethanol and tert-butyl alcohol 1:1, the pure tert-butyl alcohol each once after, each 15 minutes, with freeze drier, sample is dried to 4 hours.Sample surfaces plates the metal film of thick layer 100～150A under vacuum condition, under SEM, observes.
1.6 statistical procedures
Adopt SPSS18.0 software to carry out statistical analysis to related data, result represents by means standard deviation, the relatively employing one factor analysis of variance of statistics, and P < 0.05 has statistical significance.
2 experimental results and analysis
2.1 Folium Caryophylli extracts are to the minimum inhibitory concentration of S.suis (MIC) result of the test
By micro-dilution method, measure respectively azithromycin and Folium Caryophylli extract to the minimum inhibitory concentration of S.suis (MIC), the results are shown in Table 1.
Table 1 azithromycin and Folium Caryophylli extract are to the MIC of Streptococcus suis (unit: μ gmL
-1)
As seen from Table 1, Streptococcus suis is more responsive than Folium Caryophylli extract to azithromycin, but both all effectively suppress the growth of Streptococcus suis.
The intervention effect of 2.2 Folium Caryophylli extract 1/2MIC to S.suis biofilm
When 1/2MIC, with Folium Caryophylli extract (experimental group), azithromycin (positive controls) respectively with Streptococcus suis bacterium liquid co-cultivation 72h, result of the test shows, experimental group OD
595(0.238 ± 0.008) is starkly lower than negative control group OD
595(0.525 ± 0.014) but higher than positive controls OD
595(0.205 ± 0.004), difference is (P < 0.01) extremely significantly.Show that, when 1/2MIC, Folium Caryophylli extract is formed with obvious inhibitory action to Streptococcus suis biofilm.
The elimination effect of 2.3 Folium Caryophylli extract 1/2MIC to S.suis biofilm
Streptococcus suis biofilm, after 72h maturation, is eliminated experiment with Folium Caryophylli extract (experimental group), the azithromycin (positive control) of 1/2MIC concentration to its biofilm; Experimental result shows, experimental group OD
595(0.270 ± 0.006) is starkly lower than negative control group (OD
595(0.531 ± 0.016) but higher than positive controls OD
595(0.222 ± 0.042) difference is (P < 0.01) extremely significantly.Show when 1/2MIC, Folium Caryophylli extract has obvious elimination effect to the ripe biofilm of Streptococcus suis.
The morphological observation of 2.4 Folium Caryophylli extracts to the effect of S.suis biofilm
Under scanning electron microscope, observe, thalli morphology is clear, and bacterium number reduces, and shows that Folium Caryophylli extract has the effect of intervention biofilm formation and ripe biofilm is had to elimination effect.
Claims (10)
1. Folium Caryophylli extract suppresses Streptococcus suis or the purposes in Streptococcus suis associated diseases medicine in preparation.
2. the purposes in the medicine that Folium Caryophylli extract forms at preparation intervention Streptococcus suis biofilm.
3. Folium Caryophylli extract is eliminated the purposes in the ripe biofilm medicine of Streptococcus suis in preparation.
4. the purposes described in any one according to claim 1-3, is characterized in that: described Folium Caryophylli extract is Folium Caryophylli water alcohol mixed extract, Folium Caryophylli alcohol extract or Folium Caryophylli water extract.
5. according to purposes claimed in claim 4, it is characterized in that, the preparation method of described Folium Caryophylli water alcohol mixed extract comprises: Folium Caryophylli is extracted after soaking with Diluted Alcohol after pulverizing, and extracting solution is concentrated, obtains.
6. according to purposes claimed in claim 5, it is characterized in that: described Diluted Alcohol is 20-50% ethanol, be preferably 40% ethanol; 60min is extracted in described being extracted as at 65 ℃ of temperature.
7. a pharmaceutical composition that suppresses Streptococcus suis, is characterized in that, comprising: the Folium Caryophylli extract of effective dose and pharmaceutically acceptable adjuvant or carrier.
8. suppress the pharmaceutical composition that Streptococcus suis biofilm forms, it is characterized in that, comprising: the Folium Caryophylli extract of effective dose and pharmaceutically acceptable adjuvant or carrier.
9. a pharmaceutical composition of eliminating Streptococcus suis biofilm, is characterized in that, comprising: the Folium Caryophylli extract of effective dose and pharmaceutically acceptable adjuvant or carrier.
10. the pharmaceutical composition described in any one according to claim 7-9, is characterized in that: described Folium Caryophylli extract is Folium Caryophylli water alcohol mixed extract, Folium Caryophylli water extract or Folium Caryophylli alcohol extract; The preparation method of described Folium Caryophylli water alcohol mixed extract comprises: Folium Caryophylli is extracted after soaking with Diluted Alcohol after pulverizing, and extracting solution is concentrated, obtains.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310486414.5A CN103585277A (en) | 2013-10-17 | 2013-10-17 | Application of clove leaf extractives in inhibition of streptococcus suis and intervention and elimination of streptococcus suis biofilm |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310486414.5A CN103585277A (en) | 2013-10-17 | 2013-10-17 | Application of clove leaf extractives in inhibition of streptococcus suis and intervention and elimination of streptococcus suis biofilm |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103585277A true CN103585277A (en) | 2014-02-19 |
Family
ID=50075731
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310486414.5A Pending CN103585277A (en) | 2013-10-17 | 2013-10-17 | Application of clove leaf extractives in inhibition of streptococcus suis and intervention and elimination of streptococcus suis biofilm |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103585277A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105039493A (en) * | 2015-07-08 | 2015-11-11 | 中国农业科学院兰州兽医研究所 | Quantitative determination method of formation capability of biofilm of bacteria to be detected |
| CN106306979A (en) * | 2016-08-18 | 2017-01-11 | 华南农业大学 | Application of spice extract to inhibition and removal of food-borne pathogenic bacterium biological membrane |
| CN111419829A (en) * | 2020-03-02 | 2020-07-17 | 东北农业大学 | Application of honokiol in inhibiting streptococcus suis or biofilm thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1628799A (en) * | 2004-08-18 | 2005-06-22 | 关屹 | Clove leaf extract containing soft capsule and its preparation process |
-
2013
- 2013-10-17 CN CN201310486414.5A patent/CN103585277A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1628799A (en) * | 2004-08-18 | 2005-06-22 | 关屹 | Clove leaf extract containing soft capsule and its preparation process |
Non-Patent Citations (4)
| Title |
|---|
| 吴燕: "丁香叶总酚酸提取物最优条件的确定", 《中国畜牧兽医学会中兽医学分会2013年学术年会论文集》 * |
| 周丽光: "关东丁香化学成分研究", 《中国优秀硕士论文全文数据库 电子期刊 医药卫生科技辑》 * |
| 王玉霞: "抗菌中草药在猪预防保健中的应用研究", 《上海畜牧兽医通讯》 * |
| 韩强: "抗S.suis BF中药的筛选及大黄素对毒力基因和luxS的影响", 《中国优秀硕士论文全文数据库》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105039493A (en) * | 2015-07-08 | 2015-11-11 | 中国农业科学院兰州兽医研究所 | Quantitative determination method of formation capability of biofilm of bacteria to be detected |
| CN105039493B (en) * | 2015-07-08 | 2018-06-22 | 中国农业科学院兰州兽医研究所 | A kind of quantitative detecting method of bacterium biofilm Forming ability to be checked |
| CN106306979A (en) * | 2016-08-18 | 2017-01-11 | 华南农业大学 | Application of spice extract to inhibition and removal of food-borne pathogenic bacterium biological membrane |
| CN111419829A (en) * | 2020-03-02 | 2020-07-17 | 东北农业大学 | Application of honokiol in inhibiting streptococcus suis or biofilm thereof |
| CN111419829B (en) * | 2020-03-02 | 2023-08-18 | 东北农业大学 | Application of honokiol in inhibiting streptococcus suis or biofilm thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2022516984A (en) | Use in the manufacture of drugs to treat human papillomavirus infections of the isolated Rhodococcus louver cell wall skeleton | |
| CN108070572A (en) | A kind of width fragmentation pattern pyocinophages and its disinfection application | |
| CN102824417B (en) | New method for treating helicobacter pylori related diseases | |
| CN103585277A (en) | Application of clove leaf extractives in inhibition of streptococcus suis and intervention and elimination of streptococcus suis biofilm | |
| CN108542954A (en) | A kind of Traditional Chinese medicine gel composition of inhibitory anti-virus | |
| CN110151752A (en) | A kind of tea polyphenols composition and its preparing the application in anti-streptococcus suis drug | |
| CN111419829B (en) | Application of honokiol in inhibiting streptococcus suis or biofilm thereof | |
| WO2021093425A1 (en) | Root of membranous milkvetch-paecilomyces cicadae fermentative bacterium fermentation substance and application thereof | |
| CN108926584A (en) | The antimicrobial purposes of chimonanthea extract | |
| CN104958284B (en) | Rhein is preparing the purposes in suppressing staphylococcus xylosus biofilm medicine | |
| CN112336715B (en) | Application of inula britannica extract in preparation of medicine for treating swine pleuropneumonia actinobacillus | |
| CN110964700B (en) | Salmonella abortus phage and application thereof | |
| CN109125399A (en) | The antibacterial action of stephania tetrandra extract | |
| CN104857116B (en) | Application of the Chinese medicine garden burnet in preparing antifungal medicine synergist | |
| CN107823278B (en) | Application of aconitum sinomontanum nakai in preparation of medicine for inhibiting and killing bovine viral diarrhea virus BVDV | |
| CN104840701A (en) | Gel for treating vaginitis | |
| CN113546100B (en) | Application of pachyrhizus extract in preparation of antibacterial product | |
| Zheng et al. | Effect of Terminalia chebula Retz. extraction with water on Staphylococcus epidermidis activity and its biofilm formation | |
| CN104306477B (en) | Coptis water extract and monomer are preparing the purposes in suppressing Streptococcus suis or intervening streptococcus suis biofilm medicine | |
| CN108992478A (en) | The application of Aralia wood extract in medicine preparation | |
| CN113559270B (en) | Antibacterial composition, application and product containing same | |
| CN112891343B (en) | Application of 3-indole acetonitrile in preparing medicine for inhibiting novel coronavirus SARS-CoV-2 | |
| CN107158052A (en) | Application of the Herba Melastomatis dodecandri extract in medicine is prepared | |
| CN108130305B (en) | Bacillus subtilis and application thereof in prevention and treatment of American ginseng root rot nematode disease | |
| CN109481509B (en) | Broad-spectrum antibacterial composition and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20140219 |