CN115211514A - Deer serum rhizoma polygonati nutrient solution and preparation method thereof - Google Patents
Deer serum rhizoma polygonati nutrient solution and preparation method thereof Download PDFInfo
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- 210000002966 serum Anatomy 0.000 title claims abstract description 68
- 241000282994 Cervidae Species 0.000 title claims abstract description 67
- 235000015097 nutrients Nutrition 0.000 title claims abstract description 30
- 238000002360 preparation method Methods 0.000 title claims abstract description 12
- 210000003056 antler Anatomy 0.000 claims abstract description 37
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 33
- CHHHXKFHOYLYRE-UHFFFAOYSA-M 2,4-Hexadienoic acid, potassium salt (1:1), (2E,4E)- Chemical compound [K+].CC=CC=CC([O-])=O CHHHXKFHOYLYRE-UHFFFAOYSA-M 0.000 claims abstract description 11
- 235000012907 honey Nutrition 0.000 claims abstract description 11
- 229940069338 potassium sorbate Drugs 0.000 claims abstract description 11
- 235000010241 potassium sorbate Nutrition 0.000 claims abstract description 11
- 239000004302 potassium sorbate Substances 0.000 claims abstract description 11
- 239000008213 purified water Substances 0.000 claims abstract description 11
- 239000002994 raw material Substances 0.000 claims abstract description 9
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 35
- 238000000605 extraction Methods 0.000 claims description 26
- 239000006228 supernatant Substances 0.000 claims description 20
- 238000001914 filtration Methods 0.000 claims description 16
- 238000010992 reflux Methods 0.000 claims description 16
- 241000282985 Cervus Species 0.000 claims description 12
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 12
- 239000007788 liquid Substances 0.000 claims description 11
- 241000283007 Cervus nippon Species 0.000 claims description 10
- 239000000706 filtrate Substances 0.000 claims description 10
- 239000003480 eluent Substances 0.000 claims description 8
- 239000000463 material Substances 0.000 claims description 8
- 239000000047 product Substances 0.000 claims description 8
- 238000002386 leaching Methods 0.000 claims description 7
- 239000012528 membrane Substances 0.000 claims description 7
- OHJMTUPIZMNBFR-UHFFFAOYSA-N biuret Chemical compound NC(=O)NC(N)=O OHJMTUPIZMNBFR-UHFFFAOYSA-N 0.000 claims description 6
- 239000012141 concentrate Substances 0.000 claims description 6
- 238000002156 mixing Methods 0.000 claims description 6
- 238000001556 precipitation Methods 0.000 claims description 6
- 229920005654 Sephadex Polymers 0.000 claims description 5
- 239000012507 Sephadex™ Substances 0.000 claims description 5
- 239000000084 colloidal system Substances 0.000 claims description 5
- 238000004821 distillation Methods 0.000 claims description 5
- 238000011049 filling Methods 0.000 claims description 5
- 238000000227 grinding Methods 0.000 claims description 5
- 239000012510 hollow fiber Substances 0.000 claims description 5
- 239000002002 slurry Substances 0.000 claims description 5
- 239000011780 sodium chloride Substances 0.000 claims description 5
- 239000007787 solid Substances 0.000 claims description 5
- 230000001954 sterilising effect Effects 0.000 claims description 5
- 238000009210 therapy by ultrasound Methods 0.000 claims description 5
- 241000756042 Polygonatum Species 0.000 claims description 4
- 102000004169 proteins and genes Human genes 0.000 claims description 4
- 108090000623 proteins and genes Proteins 0.000 claims description 4
- 238000002137 ultrasound extraction Methods 0.000 claims description 4
- 238000005520 cutting process Methods 0.000 claims description 3
- 238000010828 elution Methods 0.000 claims description 3
- 230000001376 precipitating effect Effects 0.000 claims description 3
- 238000000108 ultra-filtration Methods 0.000 claims description 2
- 238000000034 method Methods 0.000 claims 2
- 239000003814 drug Substances 0.000 claims 1
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 abstract description 6
- 230000000694 effects Effects 0.000 abstract description 5
- 102000004877 Insulin Human genes 0.000 abstract description 3
- 108090001061 Insulin Proteins 0.000 abstract description 3
- 239000003102 growth factor Substances 0.000 abstract description 3
- 229940125396 insulin Drugs 0.000 abstract description 3
- 235000016709 nutrition Nutrition 0.000 abstract description 3
- 238000005336 cracking Methods 0.000 abstract description 2
- 230000036541 health Effects 0.000 abstract description 2
- 230000035764 nutrition Effects 0.000 abstract description 2
- 238000000746 purification Methods 0.000 abstract description 2
- 230000000295 complement effect Effects 0.000 abstract 1
- 210000004369 blood Anatomy 0.000 description 10
- 239000008280 blood Substances 0.000 description 10
- 238000012869 ethanol precipitation Methods 0.000 description 5
- 241001416181 Axis axis Species 0.000 description 4
- 230000036039 immunity Effects 0.000 description 3
- 238000005406 washing Methods 0.000 description 3
- 241000283026 Cervus elaphus Species 0.000 description 2
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 2
- 239000004202 carbamide Substances 0.000 description 2
- 230000002708 enhancing effect Effects 0.000 description 2
- 239000011148 porous material Substances 0.000 description 2
- 239000002244 precipitate Substances 0.000 description 2
- 102000004506 Blood Proteins Human genes 0.000 description 1
- 108010017384 Blood Proteins Proteins 0.000 description 1
- 206010070863 Toxicity to various agents Diseases 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000035622 drinking Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 230000009044 synergistic interaction Effects 0.000 description 1
- 230000001256 tonic effect Effects 0.000 description 1
- 229940126680 traditional chinese medicines Drugs 0.000 description 1
- 239000002441 uremic toxin Substances 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
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- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/14—Blood; Artificial blood
- A61K35/16—Blood plasma; Blood serum
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- A61K36/18—Magnoliophyta (angiosperms)
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Abstract
The invention discloses a deer serum rhizoma polygonati nutrient solution and a preparation method thereof, wherein the deer serum rhizoma polygonati nutrient solution is prepared from the following raw materials in parts by weight: 20 to 30 parts of deer serum, 2 to 3 parts of pilose antler, 10 to 15 parts of rhizoma polygonati, 6 to 9 parts of honey, 0.08 to 0.1 part of potassium sorbate and a proper amount of purified water; the preparation method adopts a chromatographic column purification technology and an ultrasonic cracking technology to purify and produce the deer serum, the deer antler extract, the deer antler insulin growth factor and the sealwort extract, and the three main raw materials are reasonably compatible and are in complementary cooperation to prepare the deer serum and sealwort nutrient solution which takes the deer serum as a main component and has multiple nutrition and health care effects.
Description
Technical Field
The invention belongs to the technical field of nutritional health-care food, and particularly relates to a deer serum rhizoma polygonati nutrient solution and a preparation method thereof.
Background
The pilose antler is a rare health-preserving treasure and has the function of enhancing the immunity of the human body; cornu Cervi Pantotrichum is generally soaked in wine or decocted in water for drinking. Deer blood has the functions of benefiting qi and nourishing blood, is mainly used for essence and blood deficiency, and is generally directly drunk or drunk by adding fresh deer blood into water or wine. However, the metabolic waste urea is present in deer blood in high amounts, and urea is the earliest recognized uremic toxin, and its accumulation in the body causes a series of toxic symptoms. As a traditional tonic, the pilose antler and the deer blood are used as part of the compatibility of traditional Chinese medicines, generally can only be used for soaking wine and the like, the utilization rate is low, and most of the effective components cannot be effectively utilized.
Disclosure of Invention
The invention aims to overcome the problems in the prior art and provide the deer serum rhizoma polygonati nutrient solution which plays a synergistic interaction role of each effective component, improves the antioxidant activity of the effective components and has the effects of enhancing immunity and relieving physical fatigue; the preparation method of the deer serum protein extract has the advantages that deer serum is purified, the antler is subjected to ultrasonic wall breaking treatment, the effects and the effects of all effective components are fully exerted, the product quality is stable, no precipitate exists, and the taste is good.
The technical scheme of the invention is realized as follows: a deer serum sealwort nutrient solution is prepared from the following raw materials in parts by weight: 20 to 30 parts of deer serum, 2 to 3 parts of pilose antler, 10 to 15 parts of rhizoma polygonati, 6 to 9 parts of honey, 0.08 to 0.1 part of potassium sorbate and a proper amount of purified water.
The optimized scheme of the deer serum sealwort nutrient solution is prepared from the following raw materials in parts by weight: 22 to 28 parts of deer serum, 2.2 to 2.8 parts of pilose antler, 12 to 13 parts of rhizoma polygonati, 7 to 8 parts of honey, 0.1 part of potassium sorbate and a proper amount of purified water.
The deer serum is red deer serum or sika deer serum, and the pilose antler is red deer antler or sika deer antler.
The preparation method of the deer serum rhizoma polygonati nutrient solution comprises the following steps:
(1) And (3) purifying deer serum:
(1) adding deer serum into Sephadex G-25 chromatographic column, eluting with NaCl eluent, and collecting deer serum eluate;
(2) filtering the deer serum eluate through a molecular membrane filter to obtain a purified deer serum concentrated solution;
(2) Extracting pilose antler:
(1) cutting cornu Cervi Pantotrichum into pieces, and grinding into slurry with colloid mill;
(2) adding ethanol into an ultrasonic extraction tank according to the mass ratio of the material liquid of 1.7-1.8 for extraction for 35-45 minutes;
(3) starting an ultrasonic generator to perform wall breaking treatment, wherein the ultrasonic frequency is 26-30 MHZ, the ultrasonic intensity is 80-120W, and the ultrasonic treatment time is 90-120 minutes;
(4) filtering the wall-broken treatment by a hollow fiber ultrafilter with the aperture of 2-100 nm, and separating to remove solid residue of the raw materials to obtain cornu Cervi Pantotrichum leaching filtrate;
(5) placing cornu Cervi Pantotrichum leaching filtrate into a reduced pressure distillation tank, recovering extraction medium, and making into cornu Cervi Pantotrichum extract concentrate;
(3) Extracting rhizoma polygonati:
(1) adding water into rhizoma polygonati decoction pieces for reflux extraction for three times, adding 3-4 times of water for 75-85 ℃ for reflux extraction for 2.5-3.5 hours for the first time, adding 3-4 times of water for reflux extraction for 1.5-2.5 hours for the second time at 75-85 ℃, adding 2-3 times of water for reflux extraction for 0.5-1.5 hours for the third time at 75-85 ℃, combining extracting solutions obtained in three times, and concentrating under reduced pressure to obtain rhizoma polygonati thick paste;
(2) adding ethanol into the thick polygonatum paste to ensure that the ethanol content is 50-90 percent, standing for 2-6 hours, taking supernatant, and concentrating the supernatant into polygonatum alcohol precipitation paste under reduced pressure;
(4) Preparing a nutrient solution:
(1) mixing deer serum concentrate, cornu Cervi Pantotrichum extract concentrate and rhizoma Polygonati ethanol precipitation extract, standing at 5 deg.C, refrigerating for 24 hr, collecting supernatant, centrifuging with 100 mesh sieve, and filtering to obtain supernatant;
(2) adding honey, potassium sorbate and purified water into the centrifuged supernatant, filtering, filling and sterilizing to obtain the finished product of the deer serum rhizoma polygonati nutrient solution.
Preferably, in the step (1), 0.9 mass% NaCl eluent is used for elution, a biuret reagent is used for detecting elution effluent liquid of the collected deer serum, the biuret reagent is added into the sample to show purple color and indicate that protein is eluted, the effluent liquid is continuously collected, the biuret reagent is added into the sample to show colorless color and indicate that no protein is eluted, and the collection is stopped.
Preferably, in step (1) (2), ultrafiltration is carried out by using a molecular membrane filter having a molecular weight of 50000 to 60000D.
Compared with the prior art, the invention has the beneficial effects that:
the invention adopts a chromatographic column purification technology and an ultrasonic cracking technology to purify and produce deer serum, deer antler extract, deer antler insulin growth factor and sealwort extract, and prepares the deer serum and sealwort nutrient solution which takes deer serum as a main component and has multiple nutrition and health care effects by taking the deer serum, the deer antler extract, the deer insulin growth factor and the sealwort extract as main raw materials and reasonably combining and complementing the three main raw materials.
The extracting solution prepared by the preparation method is filtered, precipitated, clarified and concentrated through multiple links, and is added with honey, potassium sorbate and a proper amount of purified water, so that the product has stable quality, no precipitate, safe eating and good taste, and is suitable for people with low immunity and easy fatigue.
Detailed Description
In order to make the technical solution of the present invention clearer, the present invention is further described in detail by the following specific examples.
Example 1:
a preparation method of a deer serum rhizoma polygonati nutrient solution comprises the following steps:
(1) And (3) purifying deer serum:
(1) adding 250 kg of red deer serum into a sephadex G-25 chromatographic column in multiple batches according to the capacity of the chromatographic column, eluting with 0.9% NaCl eluent, and collecting red deer blood washing eluate;
(2) washing sanguis Cervi, removing liquid, ultrafiltering with molecular membrane filter with molecular weight of 55000D to obtain purified sanguis Cervi concentrated solution;
(2) Extracting pilose antler:
(1) cutting 25 kg of hairy antler into pieces, and grinding the cut hairy antler pieces into hairy antler slurry by using a colloid mill;
(2) adding ethanol into an ultrasonic extraction tank according to the mass ratio of the material liquid to the material liquid of 1.75 for extraction, wherein the extraction time is 40 minutes;
(3) starting an ultrasonic generator to perform wall breaking treatment, wherein the ultrasonic frequency is 28MHZ, the ultrasonic intensity is 100W, and the ultrasonic treatment time is 100 minutes;
(4) filtering by hollow fiber ultrafilter with pore diameter of 50nm, and separating to remove solid residue to obtain cornu Cervi Pantotrichum extractive filtrate;
(5) putting the cornu Cervi Pantotrichum leaching filtrate into a reduced pressure distillation retort, recovering extraction medium, and making into cornu Cervi Pantotrichum extract concentrate;
(3) Extracting rhizoma polygonati:
(1) reflux-extracting 120 kg rhizoma Polygonati decoction pieces with water for three times, adding 3.5 times of water for the first time, and extracting at 80 + -2 deg.C for 3 hr, adding 3.5 times of water for the second time, and extracting at 80 + -2 deg.C for 2 hr, adding 2.5 times of water for the third time, and extracting at 80 + -2 deg.C for 1 hr, mixing the three extractive solutions, and concentrating under reduced pressure to obtain rhizoma Polygonati soft extract;
(2) adding ethanol into the rhizoma Polygonati soft extract to make ethanol content 70%, standing for 4 hr, collecting supernatant, and concentrating under reduced pressure to get rhizoma Polygonati ethanol precipitation extract;
(4) Preparing a nutrient solution:
(1) mixing the red deer serum concentrated solution, the red deer antler extract concentrated solution and the sealwort alcohol precipitation paste, standing, refrigerating and precipitating for 24 hours at the temperature of 5 ℃, and performing 100-mesh centrifugal filtration on the supernatant to obtain centrifugal supernatant;
(2) and adding 75 kg of honey and 1 kg of potassium sorbate into the centrifuged supernatant, adding purified water to a constant volume of 1000L, filtering, filling and sterilizing to obtain the finished product of the deer serum rhizoma polygonati nutrient solution.
Example 2:
a preparation method of a deer serum rhizoma polygonati nutrient solution comprises the following steps:
(1) And (3) purifying deer serum:
(1) adding 200 kg of red deer serum into a sephadex G-25 chromatographic column in multiple batches according to the capacity of the chromatographic column, eluting with 0.9% NaCl eluent, and collecting red deer blood washing eluate;
(2) cleaning and removing blood from Cervus Elaphus L, ultrafiltering with 50000D molecular membrane filter to obtain purified Cervus Elaphus L blood concentrated solution;
(2) Extracting pilose antler:
(1) chopping 30 kg of hairy antler and grinding the chopped hairy antler into hairy antler slurry by using a colloid mill;
(2) adding ethanol into an ultrasonic extraction tank according to the mass ratio of the material liquid to the material liquid of 1.8 for extraction, wherein the extraction time is 35 minutes;
(3) starting an ultrasonic generator to perform wall breaking treatment, wherein the ultrasonic frequency is 26MHZ, the ultrasonic intensity is 80W, and the ultrasonic treatment time is 120 minutes;
(4) filtering by hollow fiber ultrafilter with pore diameter of 80nm, and separating to remove solid residue to obtain cornu Cervi Pantotrichum extractive filtrate;
(5) putting the cornu Cervi Pantotrichum leaching filtrate into a reduced pressure distillation tank, recovering extraction medium, and making into cornu Cervi Pantotrichum extract concentrate;
(3) Extracting rhizoma polygonati:
(1) adding water into 100 kg of rhizoma polygonati decoction pieces, carrying out reflux extraction for three times, adding 3 times of water for carrying out reflux extraction for 2.5 hours at 77 +/-2 ℃ for the first time, adding 3 times of water for carrying out reflux extraction for 1.5 hours at 77 +/-2 ℃ for the second time, adding 2 times of water for carrying out reflux extraction for 0.5 hour at 77 +/-2 ℃ for the third time, combining three extracting solutions, and concentrating under reduced pressure to obtain rhizoma polygonati thick paste;
(2) adding ethanol into the rhizoma Polygonati soft extract to make ethanol content 50%, standing for 3 hr, collecting supernatant, and concentrating under reduced pressure to obtain rhizoma Polygonati ethanol precipitation extract;
(4) Preparing a nutrient solution:
(1) mixing the red deer serum concentrated solution, the red deer antler extract concentrated solution and the rhizoma polygonati alcohol precipitation paste, standing, refrigerating and precipitating for 24 hours at the temperature of 5 ℃, and performing 100-mesh centrifugal filtration on supernate to obtain centrifugal supernate;
(2) adding 70 kg of honey and 1 kg of potassium sorbate into the centrifuged supernatant, adding purified water to a constant volume of 1000L, filtering, filling and sterilizing to obtain the finished product of the deer serum rhizoma polygonati nutrient solution.
Example 3:
a preparation method of a deer serum rhizoma polygonati nutrient solution comprises the following steps:
(1) And (3) purifying deer serum:
(1) adding 300 kg of sika deer serum into a sephadex G-25 chromatographic column in multiple batches according to the capacity of the chromatographic column, eluting with a NaCl eluent with the mass concentration of 0.9%, and collecting sika deer serum eluent;
(2) ultrafiltering the sika deer serum eluate with molecular membrane filter with molecular weight of 60000D to obtain purified sika deer serum concentrated solution;
(2) Extracting pilose antler:
(1) chopping 20 kg of spotted deer antler and grinding into spotted deer antler slurry by using a colloid mill;
(2) adding ethanol into an ultrasonic leaching tank according to the mass ratio of the material liquid to the material liquid of 1.7 for leaching for 45 minutes;
(3) starting an ultrasonic generator to perform wall breaking treatment, wherein the ultrasonic frequency is 30MHZ, the ultrasonic intensity is 120W, and the ultrasonic treatment time is 90 minutes;
(4) filtering the wall-broken treatment by hollow fiber ultrafilter with aperture of 30nm, and separating to remove solid residue to obtain filtrate;
(5) putting the extracting filtrate of the spotted deer antler into a reduced pressure distillation tank, and recovering an extracting medium to prepare a concentrated solution of the spotted deer antler extract;
(3) Extracting rhizoma polygonati:
(1) adding 150 kg of sealwort decoction pieces into water for reflux extraction for three times, adding 4 times of water for reflux extraction at 83 +/-2 ℃ for 3.5 hours for the first time, adding 4 times of water for reflux extraction at 83 +/-2 ℃ for 2.5 hours for the second time, adding 3 times of water for reflux extraction at 83 +/-2 ℃ for 1.5 hours for the third time, combining extracting solutions obtained by the three times, and concentrating under reduced pressure to obtain sealwort thick paste;
(2) adding ethanol into the rhizoma Polygonati soft extract to make ethanol content 90%, standing for 5 hr, collecting supernatant, and concentrating under reduced pressure to obtain rhizoma Polygonati ethanol precipitation extract;
(4) Preparing a nutrient solution:
(1) mixing the concentrated solution of Cervus Nippon Temminck blood serum, the concentrated solution of Cervus Nippon Temminck extract and rhizoma Polygonati ethanol precipitation paste, standing at 5 deg.C, refrigerating for precipitation for 24 hr, and centrifuging the supernatant with 100 mesh to obtain centrifugal supernatant;
(2) adding 80 kg of honey and 1 kg of potassium sorbate into the centrifuged supernatant, adding purified water to a constant volume of 1000L, filtering, filling and sterilizing to obtain the finished product of the deer serum rhizoma polygonati nutrient solution.
Claims (6)
1. A deer serum rhizoma polygonati nutrient solution is prepared from the following raw materials in parts by weight: 20 to 30 parts of deer serum, 2 to 3 parts of pilose antler, 10 to 15 parts of rhizoma polygonati, 6 to 9 parts of honey, 0.08 to 0.1 part of potassium sorbate and a proper amount of purified water.
2. The deer serum rhizoma polygonati nutrient solution according to claim 1, which is characterized in that: the traditional Chinese medicine is prepared from the following raw materials in parts by weight: 22 to 28 parts of deer serum, 2.2 to 2.8 parts of pilose antler, 12 to 13 parts of rhizoma polygonati, 7 to 8 parts of honey, 0.1 part of potassium sorbate and a proper amount of purified water.
3. The deer serum rhizoma polygonati nutrient solution according to claim 2, which is characterized in that: the deer serum is red deer serum or sika deer serum, and the deer antler is red deer antler or sika deer antler.
4. The preparation method of the deer serum rhizoma polygonati nutrient solution according to any one of the claims 1, 2 or 3, comprising the following steps:
(1) And (3) purifying deer serum:
(1) adding deer serum into Sephadex G-25 chromatographic column, eluting with NaCl eluent, and collecting deer serum eluate;
(2) filtering the deer serum eluate through a molecular membrane filter to obtain a purified deer serum concentrated solution;
(2) Extracting pilose antler:
(1) cutting cornu Cervi Pantotrichum into pieces, and grinding into cornu Cervi Pantotrichum slurry with colloid mill;
(2) adding ethanol into an ultrasonic extraction tank according to the mass ratio of the material liquid of 1.7-1.8 for extraction for 35-45 minutes;
(3) starting an ultrasonic generator to perform wall breaking treatment, wherein the ultrasonic frequency is 26-30 MHZ, the ultrasonic intensity is 80-120W, and the ultrasonic treatment time is 90-120 minutes;
(4) filtering the obtained product by a hollow fiber ultrafilter with the aperture of 2-100 nm, and separating to remove solid residue to obtain cornu Cervi Pantotrichum extract filtrate;
(5) placing the cornu Cervi Pantotrichum leaching filtrate into a reduced pressure distillation tank, recovering extraction medium to obtain cornu Cervi Pantotrichum extract concentrate;
(3) Extracting rhizoma polygonati:
(1) adding water into rhizoma polygonati decoction pieces for reflux extraction for three times, adding 3-4 times of water for 75-85 ℃ for reflux extraction for 2.5-3.5 hours for the first time, adding 3-4 times of water for reflux extraction for 1.5-2.5 hours for the second time at 75-85 ℃, adding 2-3 times of water for reflux extraction for 0.5-1.5 hours for the third time at 75-85 ℃, combining extracting solutions obtained in three times, and concentrating under reduced pressure to obtain rhizoma polygonati thick paste;
(2) adding ethanol into the thick polygonatum paste to ensure that the ethanol content is 50-90 percent, standing for 2-6 hours, taking supernatant, and concentrating the supernatant into polygonatum alcohol precipitation paste under reduced pressure;
(4) Preparing a nutrient solution:
(1) mixing the deer serum concentrated solution, the pilose antler extract concentrated solution and the sealwort alcohol precipitation paste, standing, refrigerating and precipitating for 24 hours at the temperature of 5 ℃, taking the supernatant, and performing 100-mesh centrifugal filtration to obtain centrifugal supernatant;
(2) adding honey, potassium sorbate and purified water into the centrifuged supernatant, filtering, filling and sterilizing to obtain the finished product of the deer serum rhizoma polygonati nutrient solution.
5. The method for preparing the deer serum rhizoma polygonati nutrient solution according to claim 4, which is characterized by comprising the following steps: and (2) in the step (1), eluting with NaCl eluent with the mass concentration of 0.9%, detecting the collected deer serum elution effluent by using a biuret reagent, adding the biuret reagent into the sample to obtain purple, indicating that protein is eluted, continuously collecting the effluent, adding the biuret reagent into the sample to obtain colorless, indicating that no protein is eluted, and stopping collecting.
6. The method for preparing the deer serum rhizoma polygonati nutrient solution according to claim 5, which is characterized by comprising the following steps: in the step (1) (2), ultrafiltration is carried out by using a molecular membrane filter with the molecular weight of 50000-60000D.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101773521A (en) * | 2010-02-07 | 2010-07-14 | 董栗 | Method for purifying deer serum |
| CN106214989A (en) * | 2016-09-06 | 2016-12-14 | 贵州科博生物科技有限公司 | A kind of defying age, the deer's blood health-care medicated wine improving memory and preparation method thereof |
| CN110236048A (en) * | 2019-07-04 | 2019-09-17 | 福建欢鹿实业有限公司 | Gelatine matter drinks and preparation method thereof |
| CN110760423A (en) * | 2019-11-26 | 2020-02-07 | 新疆金鹿药业有限公司 | Deer blood wine for delaying aging and regulating immunity, and its preparation method |
| CN110771894A (en) * | 2019-12-13 | 2020-02-11 | 内蒙古中鹿科技有限责任公司 | Pilose antler oral liquid and preparation method thereof |
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101773521A (en) * | 2010-02-07 | 2010-07-14 | 董栗 | Method for purifying deer serum |
| CN106214989A (en) * | 2016-09-06 | 2016-12-14 | 贵州科博生物科技有限公司 | A kind of defying age, the deer's blood health-care medicated wine improving memory and preparation method thereof |
| CN110236048A (en) * | 2019-07-04 | 2019-09-17 | 福建欢鹿实业有限公司 | Gelatine matter drinks and preparation method thereof |
| CN110760423A (en) * | 2019-11-26 | 2020-02-07 | 新疆金鹿药业有限公司 | Deer blood wine for delaying aging and regulating immunity, and its preparation method |
| CN110771894A (en) * | 2019-12-13 | 2020-02-11 | 内蒙古中鹿科技有限责任公司 | Pilose antler oral liquid and preparation method thereof |
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Application publication date: 20221021 |