CN115153016A - Sea cucumber polypeptide chelated zinc oral liquid and preparation method thereof - Google Patents
Sea cucumber polypeptide chelated zinc oral liquid and preparation method thereof Download PDFInfo
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- CN115153016A CN115153016A CN202110365833.8A CN202110365833A CN115153016A CN 115153016 A CN115153016 A CN 115153016A CN 202110365833 A CN202110365833 A CN 202110365833A CN 115153016 A CN115153016 A CN 115153016A
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- 241000251511 Holothuroidea Species 0.000 title claims abstract description 82
- 229920001184 polypeptide Polymers 0.000 title claims abstract description 79
- 102000004196 processed proteins & peptides Human genes 0.000 title claims abstract description 79
- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 79
- 229940038879 chelated zinc Drugs 0.000 title claims abstract description 59
- 239000007788 liquid Substances 0.000 title claims abstract description 50
- 238000002360 preparation method Methods 0.000 title claims abstract description 15
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 claims abstract description 36
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 35
- 241000222336 Ganoderma Species 0.000 claims abstract description 11
- 240000000588 Hericium erinaceus Species 0.000 claims abstract description 11
- 235000007328 Hericium erinaceus Nutrition 0.000 claims abstract description 11
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 claims abstract description 11
- 235000003140 Panax quinquefolius Nutrition 0.000 claims abstract description 11
- 241001506047 Tremella Species 0.000 claims abstract description 11
- 240000006794 Volvariella volvacea Species 0.000 claims abstract description 11
- 235000008434 ginseng Nutrition 0.000 claims abstract description 11
- 229940109850 royal jelly Drugs 0.000 claims abstract description 11
- 241000233866 Fungi Species 0.000 claims abstract description 9
- 241000234435 Lilium Species 0.000 claims abstract description 9
- 235000017784 Mespilus germanica Nutrition 0.000 claims abstract description 9
- 244000182216 Mimusops elengi Species 0.000 claims abstract description 9
- 235000000560 Mimusops elengi Nutrition 0.000 claims abstract description 9
- 235000007837 Vangueria infausta Nutrition 0.000 claims abstract description 9
- 239000008367 deionised water Substances 0.000 claims description 19
- 229910021641 deionized water Inorganic materials 0.000 claims description 19
- 238000006243 chemical reaction Methods 0.000 claims description 17
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 15
- 239000002244 precipitate Substances 0.000 claims description 15
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims description 15
- 229960001763 zinc sulfate Drugs 0.000 claims description 15
- 229910000368 zinc sulfate Inorganic materials 0.000 claims description 15
- 241000208340 Araliaceae Species 0.000 claims description 10
- 238000002156 mixing Methods 0.000 claims description 10
- 239000000843 powder Substances 0.000 claims description 10
- 240000000599 Lentinula edodes Species 0.000 claims description 9
- 235000001715 Lentinula edodes Nutrition 0.000 claims description 7
- 230000009920 chelation Effects 0.000 claims description 6
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 claims description 5
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 5
- 235000011114 ammonium hydroxide Nutrition 0.000 claims description 5
- 239000012295 chemical reaction liquid Substances 0.000 claims description 5
- 238000004587 chromatography analysis Methods 0.000 claims description 5
- 238000001816 cooling Methods 0.000 claims description 5
- 239000004744 fabric Substances 0.000 claims description 5
- 238000011049 filling Methods 0.000 claims description 5
- 238000001914 filtration Methods 0.000 claims description 5
- 238000004108 freeze drying Methods 0.000 claims description 5
- 238000010438 heat treatment Methods 0.000 claims description 5
- 238000007873 sieving Methods 0.000 claims description 5
- 238000002791 soaking Methods 0.000 claims description 5
- 239000007921 spray Substances 0.000 claims description 4
- 238000000034 method Methods 0.000 claims description 3
- 241000221377 Auricularia Species 0.000 claims description 2
- 230000007613 environmental effect Effects 0.000 claims description 2
- 239000011701 zinc Substances 0.000 abstract description 15
- 229910052725 zinc Inorganic materials 0.000 abstract description 15
- 238000010521 absorption reaction Methods 0.000 abstract description 3
- 235000001674 Agaricus brunnescens Nutrition 0.000 abstract description 2
- 244000131316 Panax pseudoginseng Species 0.000 abstract 1
- 230000000694 effects Effects 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- 150000003751 zinc Chemical class 0.000 description 3
- 206010028980 Neoplasm Diseases 0.000 description 2
- 206010048259 Zinc deficiency Diseases 0.000 description 2
- PTFCDOFLOPIGGS-UHFFFAOYSA-N Zinc dication Chemical compound [Zn+2] PTFCDOFLOPIGGS-UHFFFAOYSA-N 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 239000013522 chelant Substances 0.000 description 2
- 238000010668 complexation reaction Methods 0.000 description 2
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 2
- 230000036039 immunity Effects 0.000 description 2
- 229910052751 metal Inorganic materials 0.000 description 2
- 239000002184 metal Substances 0.000 description 2
- 229910021645 metal ion Inorganic materials 0.000 description 2
- 230000003647 oxidation Effects 0.000 description 2
- 238000007254 oxidation reaction Methods 0.000 description 2
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 208000002874 Acne Vulgaris Diseases 0.000 description 1
- 206010012559 Developmental delay Diseases 0.000 description 1
- 206010013883 Dwarfism Diseases 0.000 description 1
- 241001482237 Pica Species 0.000 description 1
- 206010052428 Wound Diseases 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 206010000496 acne Diseases 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 206010061428 decreased appetite Diseases 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 208000035475 disorder Diseases 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 230000036244 malformation Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000001568 sexual effect Effects 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 230000009469 supplementation Effects 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 230000029663 wound healing Effects 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L21/00—Marmalades, jams, jellies or the like; Products from apiculture; Preparation or treatment thereof
- A23L21/20—Products from apiculture, e.g. royal jelly or pollen; Substitutes therefor
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/16—Inorganic salts, minerals or trace elements
- A23L33/165—Complexes or chelates
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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Abstract
The invention relates to a sea cucumber polypeptide chelated zinc oral liquid, in particular to a sea cucumber polypeptide chelated zinc oral liquid and a preparation method thereof, the sea cucumber polypeptide chelated zinc oral liquid comprises the following components, by weight, 10-30 parts of lucid ganoderma, 10-30 parts of ginseng, 5-20 parts of hericium erinaceus, 5-20 parts of mushrooms, 5-20 parts of straw mushrooms, 5-20 parts of black fungus, 5-20 parts of lily, 5-20 parts of medlar, 5-20 parts of tremella, 800-1200 parts of water, 20-40 parts of sea cucumber polypeptide chelated zinc powder, and 5-20 parts of royal jelly; the oral liquid of the formula improves the zinc absorption efficiency of organisms and improves the use value of the sea cucumber.
Description
Technical Field
The invention relates to a sea cucumber polypeptide chelated zinc oral liquid, in particular to a sea cucumber polypeptide chelated zinc oral liquid and a preparation method thereof.
Background
It is known that zinc is one of the trace elements essential to human body, has a close relationship with the synthesis of DNA, RNA and protein, and has a certain effect on the inhibition of free radicals. When the zinc is deficient in human body, the children can have the phenomena of growth and development retardation, sexual maturity disorder, poor wound healing capability, inappetence, taste decline, pica and the like, and the dwarfism second sex character development disorder and hypofunction can be caused by serious deficiency; the zinc deficiency of adults easily causes that wounds are not easy to heal, and acne and the like easily occur to skin; the pregnant woman is easy to have low weight and even malformation due to zinc deficiency. Therefore, the effective supplement of zinc is vital to the health of people; at present, zinc is generally supplemented by an oral liquid method, but most of zinc element in the oral liquid is directly lost, and only a small part of zinc element is absorbed, so that not only is resource waste caused, but also the zinc element supplementation is influenced.
Disclosure of Invention
The invention aims to provide a sea cucumber polypeptide chelated zinc oral liquid and a preparation method thereof, aiming at the defects and shortcomings of the prior art.
In order to realize the purpose, the invention adopts the technical scheme that:
the sea cucumber polypeptide chelated zinc oral liquid comprises the following components in percentage by weight,
10-30 parts of lucid ganoderma, 10-30 parts of ginseng, 5-20 parts of hericium erinaceus, 5-20 parts of lentinus edodes, 5-20 parts of straw mushroom, 5-20 parts of black fungus, 5-20 parts of lily, 5-20 parts of medlar, 5-20 parts of tremella, 800-1200 parts of water, 20-40 parts of sea cucumber polypeptide chelated zinc powder and 5-20 parts of royal jelly.
Further, the method comprises the following steps:
a. soaking Ganoderma, ginseng radix, hericium Erinaceus, lentinus Edodes, straw Mushroom, auricularia, bulbus Lilii, fructus Lycii, and Tremella in water, decocting with strong fire for 2-4h, centrifuging at 5000-8000r/min for 10-20min, and filtering with 80 mesh filter cloth to obtain concentrated extractive solution;
b. uniformly mixing a predetermined amount of the sea cucumber polypeptide chelated zinc powder and royal jelly with the concentrated extracting solution obtained in the step a to obtain oral liquid;
c. and c, filling the oral liquid uniformly mixed in the step b, and spraying and heating the oral liquid by using hot water at the temperature of 60-70 ℃ to keep the temperature of the oral liquid at 58-65 ℃ for 5-10min.
Further, the preparation method of the sea cucumber polypeptide chelated zinc powder in the step b comprises the following steps:
a1, adding sea cucumber polypeptide powder which is 0.04-0.08 times of the weight of the first part of deionized water into the first part of deionized water, and fully dissolving to obtain sea cucumber polypeptide liquid;
b1, adding zinc sulfate powder with the weight 0.02-0.04 time of that of the second part of deionized water into the second part of deionized water, and fully dissolving to obtain a zinc sulfate solution;
c1, respectively taking the sea cucumber polypeptide solution and the zinc sulfate solution which are prepared in the same volume, uniformly mixing, and adjusting the pH value to 5.0-6.0 by using dilute hydrochloric acid or dilute ammonia water;
d1, carrying out chelation reaction on the liquid obtained in the step c1 at the constant temperature of 70-80 ℃ for 40-60min, and cooling to obtain a sea cucumber polypeptide chelated zinc reaction liquid;
e1, adding an absolute ethanol solution into the sea cucumber polypeptide chelated zinc reaction solution obtained in the step a until the ethanol concentration in the reaction solution reaches 60%, and standing overnight in a chromatography cabinet at the ambient temperature of 4-6 ℃ to obtain a sea cucumber polypeptide chelated zinc precipitate;
f1, centrifuging the sea cucumber polypeptide chelated zinc precipitate obtained in the step e1 for 10-15min under the condition of 5000-6000r/min, collecting the precipitate, freeze-drying, and sieving with a 80-mesh sieve to obtain the sea cucumber polypeptide chelated zinc powder.
Compared with the prior art, the invention has the beneficial effects that:
1. the sea cucumber polypeptide has the effects of resisting oxidation, resisting tumor, regulating immunity and the like;
2. after the zinc is added through the chelation reaction, the chelated zinc salt is a product formed by the complexation of zinc ions in soluble metal salt and polypeptide under specific conditions and the combination of coordination bonds and ionic bonds, and the unique structure of five-membered ring or six-membered ring with coexisting ionic bonds and coordination bonds is formed by the action of amino and hydroxyl on alpha-carbon and metal ions, so that the zinc-supplementing effect is good, and the zinc absorption rate of an organism is improved;
3. the product improves the economic value and the utilization rate of the sea cucumber and has profound significance for the deep processing of the sea cucumber.
Detailed Description
The present invention will now be described in detail with reference to specific examples, which are provided for illustration only and are not intended to be limiting.
Example 1:
the sea cucumber polypeptide chelated zinc oral liquid is prepared from the following components in parts by weight:
the weight of each part of the following components is 1kg;
30 parts of lucid ganoderma, 30 parts of ginseng, 20 parts of hericium erinaceus, 20 parts of lentinus edodes, 20 parts of straw mushroom, 13 parts of black fungus, 20 parts of lily, 20 parts of medlar, 5 parts of tremella, 1000 parts of water, 30 parts of sea cucumber polypeptide chelated zinc powder and 5 parts of royal jelly.
The preparation method of the sea cucumber polypeptide chelated zinc oral liquid is realized by the following steps:
a. soaking 30 parts of lucid ganoderma, 30 parts of ginseng, 20 parts of hericium erinaceus, 20 parts of mushroom, 20 parts of straw mushroom, 13 parts of black fungus, 20 parts of lily, 20 parts of medlar and 5 parts of tremella in 1000 parts of water, decocting for 3 hours with strong fire, centrifuging for 20 minutes under the condition of 5000r/min, and filtering by using 80-mesh filter cloth to obtain a concentrated extracting solution;
b. uniformly mixing 30 parts of sea cucumber polypeptide chelated zinc powder, 5 parts of royal jelly and the concentrated extract obtained in the step a to obtain oral liquid; the preparation method of the sea cucumber polypeptide chelated zinc powder comprises the following steps: a1, adding sea cucumber polypeptide powder which is 0.06 time of the weight of the first part of deionized water into the first part of deionized water, and fully dissolving to obtain sea cucumber polypeptide liquid; b1, adding zinc sulfate powder with the weight 0.04 times that of the second part of deionized water into the second part of deionized water, and fully dissolving to obtain a zinc sulfate solution; c1, respectively taking the sea cucumber polypeptide solution and the zinc sulfate solution which are prepared in the same volume, uniformly mixing, and adjusting the pH value to 6.0 by using dilute hydrochloric acid or dilute ammonia water; d1, carrying out chelation reaction on the liquid obtained in the step c1 for 40min at the constant temperature of 70 ℃, and cooling to obtain a sea cucumber polypeptide zinc chelate reaction liquid; e1, adding an absolute ethanol solution into the sea cucumber polypeptide chelated zinc reaction solution obtained in the step a until the ethanol concentration in the reaction solution reaches 60%, and standing overnight in a chromatography cabinet with the environmental temperature of 4 ℃ to obtain sea cucumber polypeptide chelated zinc precipitate; f1, centrifuging the sea cucumber polypeptide chelated zinc precipitate obtained in the step e1 for 10min under the condition of 6000r/min, collecting the precipitate, freeze-drying, and sieving by a sieve of 80 meshes to obtain the sea cucumber polypeptide chelated zinc powder.
c. And c, filling the oral liquid uniformly mixed in the step b, and then performing spray heating treatment by using hot water at 70 ℃ to keep the temperature of the oral liquid at 65 ℃ for 5min.
Example 2:
the sea cucumber polypeptide chelated zinc oral liquid is prepared from the following components in parts by weight:
the weight of each part of the following components is 1kg;
10 parts of lucid ganoderma, 10 parts of ginseng, 5 parts of hericium erinaceus, 5 parts of lentinus edodes, 13 parts of straw mushroom, 20 parts of black fungus, 5 parts of lily, 13 parts of medlar, 13 parts of tremella, 1200 parts of water, 20 parts of sea cucumber polypeptide chelated zinc powder and 13 parts of royal jelly.
The preparation method of the sea cucumber polypeptide chelated zinc oral liquid is realized by the following steps:
a. soaking 10 parts of lucid ganoderma, 10 parts of ginseng, 5 parts of hericium erinaceus, 5 parts of shiitake mushroom, 13 parts of straw mushroom, 20 parts of black fungus, 5 parts of lily, 13 parts of medlar and 13 parts of tremella in 1200 parts of water, decocting for 4 hours with strong fire, centrifuging for 10 minutes under the condition of 6500r/min, and filtering by using 80-mesh filter cloth to obtain a concentrated extracting solution;
b. uniformly mixing 20 parts of sea cucumber polypeptide chelated zinc powder, 13 parts of royal jelly and the concentrated extract obtained in the step a to obtain oral liquid; the preparation method of the sea cucumber polypeptide chelated zinc powder comprises the following steps: a1, adding sea cucumber polypeptide powder with the weight 0.08 times that of the first part of deionized water into the first part of deionized water, and fully dissolving to obtain sea cucumber polypeptide liquid; b1, adding zinc sulfate powder with the weight 0.02 time that of the second part of deionized water into the second part of deionized water, and fully dissolving to obtain a zinc sulfate solution; c1, respectively taking the sea cucumber polypeptide solution and the zinc sulfate solution which are prepared in the same volume, uniformly mixing, and adjusting the pH value to 5.5 by using dilute hydrochloric acid or dilute ammonia water; d1, carrying out chelation reaction on the liquid obtained in the step c1 for 60min at the constant temperature of 80 ℃, and cooling to obtain a sea cucumber polypeptide zinc chelate reaction liquid; e1, adding an absolute ethanol solution into the sea cucumber polypeptide chelated zinc reaction solution obtained in the step a until the ethanol concentration in the reaction solution reaches 60%, and standing overnight in a chromatography cabinet with the ambient temperature of 5 ℃ to obtain sea cucumber polypeptide chelated zinc precipitate; f1, centrifuging the sea cucumber polypeptide chelated zinc precipitate obtained in the step e1 for 15min under the condition of 5000r/min, collecting the precipitate, freeze-drying, and sieving by using a 80-mesh sieve to obtain the sea cucumber polypeptide chelated zinc powder.
c. And c, filling the oral liquid uniformly mixed in the step b, and then performing spray heating treatment by using hot water at 60 ℃ to keep the temperature of the oral liquid at 58 ℃ for 10min.
Example 3:
the sea cucumber polypeptide chelated zinc oral liquid is prepared from the following components in parts by weight:
the weight of each part of the following components is 1kg;
20 parts of lucid ganoderma, 20 parts of ginseng, 13 parts of hericium erinaceus, 13 parts of lentinus edodes, 5 parts of straw mushroom, 13 parts of black fungus, 13 parts of lily, 5 parts of medlar, 20 parts of tremella, 800 parts of water, 40 parts of sea cucumber polypeptide chelated zinc powder and 20 parts of royal jelly.
The preparation method of the sea cucumber polypeptide chelated zinc oral liquid is realized by the following steps:
a. soaking 20 parts of lucid ganoderma, 20 parts of ginseng, 13 parts of hericium erinaceus, 13 parts of shiitake mushroom, 5 parts of straw mushroom, 13 parts of black fungus, 13 parts of lily, 5 parts of medlar and 20 parts of tremella in 800 parts of water, decocting for 2 hours with strong fire, centrifuging for 15min at 8000r/min, and filtering by using 80-mesh filter cloth to obtain a concentrated extracting solution;
b. uniformly mixing 40 parts of sea cucumber polypeptide chelated zinc powder, 20 parts of royal jelly and the concentrated extract obtained in the step a to obtain oral liquid; the preparation method of the sea cucumber polypeptide chelated zinc powder comprises the following steps: a1, adding sea cucumber polypeptide powder with the weight 0.04 times of that of the first part of deionized water into the first part of deionized water, and fully dissolving to obtain sea cucumber polypeptide liquid; b1, adding zinc sulfate powder which is 0.03 time of the weight of the second part of deionized water into the second part of deionized water, and fully dissolving to obtain a zinc sulfate solution; c1, respectively taking the sea cucumber polypeptide liquid with the same volume as the prepared zinc sulfate solution, uniformly mixing, and adjusting the pH value to 5.0 by using dilute hydrochloric acid or dilute ammonia water; d1, carrying out chelation reaction on the liquid obtained in the step c1 for 50min at the constant temperature of 75 ℃, and cooling to obtain a sea cucumber polypeptide chelated zinc reaction liquid; e1, adding an absolute ethanol solution into the sea cucumber polypeptide chelated zinc reaction solution obtained in the step a until the ethanol concentration in the reaction solution reaches 60%, and standing overnight in a chromatography cabinet at the ambient temperature of 6 ℃ to obtain a sea cucumber polypeptide chelated zinc precipitate; f1, centrifuging the sea cucumber polypeptide chelated zinc precipitate obtained in the step e1 for 13min under the condition of 5500r/min, collecting the precipitate, freeze-drying, and sieving by a sieve of 80 meshes to obtain the sea cucumber polypeptide chelated zinc powder.
c. And c, filling the oral liquid uniformly mixed in the step b, and then performing spray heating treatment by using hot water at 65 ℃ to keep the temperature of the oral liquid at 62 ℃ for 7min.
Compared with the prior art, the invention has the beneficial effects that:
1. the sea cucumber polypeptide has the effects of resisting oxidation, resisting tumors, regulating immunity and the like;
2. after the chelating reaction is carried out and zinc is added, the chelating zinc salt is a product formed by the complexation reaction of zinc ions in soluble metal salt and polypeptide under specific conditions and the combination of a coordination bond and an ionic bond, and the unique structure of a five-membered ring or a six-membered ring with the coexistence of the ionic bond and the coordination bond is formed by the action of amino and hydroxyl on alpha-carbon and the metal ions, so that the chelating zinc salt has a good zinc supplementing effect and improves the zinc absorption rate of an organism;
3. the product improves the economic value and the utilization rate of the sea cucumber and has profound significance for the deep processing of the sea cucumber.
The above description is for convenience only and various simple changes and modifications made by those skilled in the art without departing from the spirit of the invention should be considered within the scope of the invention.
Claims (3)
1. A sea cucumber polypeptide chelated zinc oral liquid is characterized in that: the formula of the sea cucumber polypeptide chelated zinc oral liquid comprises the following components in percentage by weight,
10-30 parts of lucid ganoderma, 10-30 parts of ginseng, 5-20 parts of hericium erinaceus, 5-20 parts of lentinus edodes, 5-20 parts of straw mushroom, 5-20 parts of black fungus, 5-20 parts of lily, 5-20 parts of medlar, 5-20 parts of tremella, 800-1200 parts of water, 20-40 parts of sea cucumber polypeptide chelated zinc powder and 5-20 parts of royal jelly.
2. The preparation method of the sea cucumber polypeptide chelated zinc oral liquid according to claim 1, characterized by comprising the following steps: the method comprises the following steps:
a. soaking predetermined amount of Ganoderma, ginseng radix, hericium Erinaceus, lentinus Edodes, straw Mushroom, auricularia, bulbus Lilii, fructus Lycii, and Tremella in water, decocting with strong fire for 2-4 hr, centrifuging at 5000-8000r/min for 10-20min, and filtering with 80 mesh filter cloth to obtain concentrated extractive solution;
b. uniformly mixing a predetermined amount of the sea cucumber polypeptide chelated zinc powder and royal jelly with the concentrated extracting solution obtained in the step a to obtain oral liquid;
c. and c, filling the oral liquid uniformly mixed in the step b, and then performing spray heating treatment by using hot water at the temperature of 60-70 ℃ to keep the temperature of the oral liquid at 58-65 ℃ for 5-10min.
3. The preparation method of the sea cucumber polypeptide chelated zinc oral liquid according to claim 2, characterized by comprising the following steps:
the preparation method of the sea cucumber polypeptide chelated zinc powder in the step b comprises the following steps:
a1, adding 0.04-0.08 times of the weight of the sea cucumber polypeptide powder into the first part of deionized water, and fully dissolving to obtain a sea cucumber polypeptide liquid;
b1, adding zinc sulfate powder with the weight 0.02-0.04 time of that of the second part of deionized water into the second part of deionized water, and fully dissolving to obtain a zinc sulfate solution;
c1, respectively taking the sea cucumber polypeptide liquid with the same volume as the prepared zinc sulfate solution, uniformly mixing, and adjusting the pH value to 5.0-6.0 by using dilute hydrochloric acid or dilute ammonia water;
d1, carrying out chelation reaction on the liquid obtained in the step c1 for 40-60min at a constant temperature of 70-80 ℃, and cooling to obtain a sea cucumber polypeptide chelated zinc reaction liquid;
e1, adding an absolute ethanol solution into the sea cucumber polypeptide chelated zinc reaction solution obtained in the step a until the ethanol concentration in the reaction solution reaches 60%, and standing overnight in a chromatography cabinet with the environmental temperature of 4-6 ℃ to obtain sea cucumber polypeptide chelated zinc precipitate;
f1, centrifuging the sea cucumber polypeptide chelated zinc precipitate obtained in the step e1 for 10-15min under the condition of 5000-6000r/min, collecting the precipitate, freeze-drying, and sieving with a 80-mesh sieve to obtain the sea cucumber polypeptide chelated zinc powder.
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