CN102167752B - Preparation method of water-soluble soybean polysaccharide ferrous coordination compound - Google Patents
Preparation method of water-soluble soybean polysaccharide ferrous coordination compound Download PDFInfo
- Publication number
- CN102167752B CN102167752B CN2011101325045A CN201110132504A CN102167752B CN 102167752 B CN102167752 B CN 102167752B CN 2011101325045 A CN2011101325045 A CN 2011101325045A CN 201110132504 A CN201110132504 A CN 201110132504A CN 102167752 B CN102167752 B CN 102167752B
- Authority
- CN
- China
- Prior art keywords
- water
- soybean polysaccharide
- soluble soybean
- ferrous
- iron
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines Containing Plant Substances (AREA)
- Beans For Foods Or Fodder (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention discloses a preparation method of a water-soluble soybean polysaccharide ferrous coordination compound, belonging to the technical field of a polysaccharide coordination compound. The preparation method comprises the steps of dissolving water-soluble soybean polysaccharide and a catalytic agent; putting the water-soluble soybean polysaccharide and the catalytic agent into water bath under 40-90DEG C; dropwise adding ferrous sulfate solution during stirring; simultaneously dropwise adding hydrochloric acid solution or sodium acetate solution; keeping the PH value of reaction solution at 2-5; continuously reaction for 0.5-3h during stirring after dropwise adding the ferrous sulfate; performing the alcohol deposition to the reaction solution after cooling; centrifugally separating; and washing and drying obtained precipitate to obtain the water-soluble soybean polysaccharide ferrous coordination compound. The water-soluble soybean polysaccharide ferrous coordination compound is flaxen powder, the iron content is 10-30%, ferrous iron is hard to oxidize, the bioavailability is high, the source of raw materials is easy to obtain, and the price is low, so that the compound can be developed into an iron-strengthened food additive and a nutritional type oral iron supplement agent.
Description
Technical field
The invention belongs to the polysaccharide complex technical field, the preparation method of the ferrous title complex of particularly a kind of water soluble soybean polysaccharide.
Background technology
Soybean polysaccharide is from the resulting water-soluble polysaccharides material of pulse family soybean cotyledon part.Because it is cheap and easy to get, and has many superior physiological functions and food function characteristic, has been widely used in each field of food.
Iron is one of trace element essential in the human body, is the core of oxyphorase and myohaemoglobin, and many in the cell have the enzyme of critical function also all to contain iron, is indispensable material in the many normal physiological processes of human body.Sideropenia can cause multiple disease, and iron-deficiency anaemia has become one of modal disease in the world, and human beings'health in serious threat, how to prevent and treat iron-deficiency anaemia more and more to receive people's attention.The research of existing many Polyferose title complexs both at home and abroad is like the benefit chalybeate niferex that has gone on the market, Iron-Dextrin Complex, Lalgine iron, angelica polysaccharide iron, Dihuang polysaccharide iron, GPS's iron etc.; Research shows that Polyferose class title complex not only can be avoided GI irritation, and it is little also to have a spinoff; Solubleness is good, the iron-holder advantages of higher, and the part polysaccharide of vegetable polysaccharides iron complex has other multiple efficacies concurrently; After the vegetable polysaccharides iron complex discharges in vivo, not only can mend iron, can also bring into play the effect of part; Body is played double protection, is a kind of up-and-coming benefit chalybeate.
After polysaccharide and ferric iron form title complex; The iron of this form must separate with organic moiety; And just can be absorbed after being reduced into ferrous iron, thereby, research water soluble soybean polysaccharide and ferrous title complex; Be expected to be developed to the foodstuff additive and the oral benefit chalybeate of nutritional type of reinforced iron, improve its bioavailability.
Summary of the invention
The object of the invention is to overcome the shortcoming of prior art; The preparation method of the ferrous title complex of a kind of water soluble soybean polysaccharide is provided, and its iron-holder is high, ferrously is difficult for oxidation; Bioavailability is high; Raw material sources are easy to get, and are cheap, are expected to be developed to the foodstuff additive and the oral benefit chalybeate of nutritional type of reinforced iron.
For reaching above-mentioned purpose, the present invention adopts following technical scheme:
(1) with after water soluble soybean polysaccharide and the catalyst dissolution, place 40 ~ 90 ℃ of water-baths, stir and drip copperas solution down, while dripping hydrochloric acid or sodium acetate soln, keeping the pH of reaction solution is 2 ~ 5, drips the continued stirring reaction 0.5 ~ 3 hour;
(2) reaction solution cooling back alcohol is analysed, spinning, and the gained deposition gets the ferrous title complex of water soluble soybean polysaccharide after washing, drying;
Said catalyzer is Citrate trianion or tartrate.
Said Citrate trianion is trisodium citrate or citric acid tri potassium, and said tartrate is sodium tartrate or soluble tartrate.
The mass ratio of said water soluble soybean polysaccharide and catalyzer is 0.25:1 ~ 5:1.
It is in reaction solution, to add absolute ethyl alcohol that said alcohol is analysed, and separates out deposition.
Said washing is alcohol to be analysed the gained deposition wash with 95 % ethanol, absolute ethyl alcohol and anhydrous propanone successively.
Said drying is warm air drying, vacuum-drying, lyophilize or spraying drying.
The rate of addition of adjustment hydrochloric acid or sodium acetate soln, keeping the pH of reaction solution is 2 ~ 5, and the present invention does not limit the dripping quantity of ferrous sulfate, and preferred ferrous ion and water soluble soybean polysaccharide mass ratio are 1:1.
The present invention is with respect to advantage and beneficial effect that prior art had.
(1) the present invention utilizes water soluble soybean polysaccharide and ferrous sulfate in the bean dregs to prepare the ferrous title complex of water soluble soybean polysaccharide first.
(2) the ferrous title complex of water soluble soybean polysaccharide utilizes the phenanthroline spectrophotometry to measure its iron-holder at 10 ~ 30 %, and iron-holder is high, and is safer and more effective as the foodstuff additive and the oral benefit chalybeate of nutritional type of reinforced iron.The solution of the ferrous title complex of water soluble soybean polysaccharide is placed one day ferrous iron and can be oxidized to ferric iron and be reddish-brown, and stability is high, and ferrous in the ferrous title complex need not restore, and can directly be absorbed by the body.
(3) the ferrous title complex of water soluble soybean polysaccharide is the vegetable polysaccharides iron complex; The part polysaccharide of the ferrous title complex of vegetable polysaccharides has other multiple efficacies concurrently; After water soluble soybean polysaccharide-iron complex discharges in vivo, not only can mend iron, can also bring into play the effect of part.
Embodiment
Embodiment 1
Accurately take by weighing the 0.100g water soluble soybean polysaccharide and the 0.020g trisodium citrate is dissolved in the 15.00mL water, place 60 ℃ of water-baths, slowly drip the copperas solution 2mL of iron content 0.89mmol/mL; The hydrochloric acid soln adjusting pH that drips 1wt% simultaneously is 4.6; Stop to drip continued stirring reaction 1h, 1h is analysed with absolute ethyl alcohol alcohol, 5000r/min spinning 5min in the cooling back; Deposition does not contain ferrous ion with 95% ethanol, absolute ethyl alcohol and anhydrous propanone washing until washing lotion.Get SSPS-Fe (II) title complex after the drying, its iron-holder is 8.5 %.
Embodiment 2
Accurately take by weighing 0.100g water soluble soybean polysaccharide and 0.400g trisodium citrate and be dissolved in the 15.00mL water, other are with embodiment 1, and gained title complex iron-holder is 6.3 %.
Embodiment 3
Accurately take by weighing the 0.100g water soluble soybean polysaccharide and the 0.100g trisodium citrate is dissolved in the 15.00mL water, place 40 ℃ of water-baths, other are with embodiment 1, and gained title complex iron-holder is 16.6 %.
Embodiment 4
Accurately take by weighing the 0.100g water soluble soybean polysaccharide and the 0.100g trisodium citrate is dissolved in the 15.00mL water, place 90 ℃ of water-baths, other are with embodiment 1, and gained title complex iron-holder is 17.9 %.
Embodiment 5
Accurately taking by weighing 0.100g water soluble soybean polysaccharide and 0.100g trisodium citrate is dissolved in the 15.00mL water; Place 60 ℃ of water-baths; Slowly drip the copperas solution 2mL of iron content 0.89mmol/mL, the hydrochloric acid soln adjusting pH that drips 1wt% simultaneously is 4.6, stops to drip continued stirring reaction 0.5 h; Other are with embodiment 1, and gained title complex iron-holder is 13.9 %.
Embodiment 6
Accurately taking by weighing 0.100g water soluble soybean polysaccharide and 0.100g trisodium citrate is dissolved in the 15.00mL water; Place 60 ℃ of water-baths; Slowly drip the copperas solution 2mL of iron content 0.89mmol/mL, the hydrochloric acid soln adjusting pH that drips 1wt% simultaneously is 4.6, stops to drip continued stirring reaction 3.0 h; Other are with embodiment 1, and gained title complex iron-holder is 13.3 %.
Embodiment 7
Accurately taking by weighing 0.100g water soluble soybean polysaccharide and 0.100g trisodium citrate is dissolved in the 15.00mL water; Place 60 ℃ of water-baths; Slowly drip the copperas solution 2mL of iron content 0.89mmol/mL, dripping 1wt% hydrochloric acid adjusting pH simultaneously is 2, stops to drip continued stirring reaction 1.0 h; Other are with embodiment 1, and gained title complex iron-holder is 5.8 %.
Embodiment 8
Accurately taking by weighing 0.100g water soluble soybean polysaccharide and 0.100g trisodium citrate is dissolved in the 15.00mL water; Place 60 ℃ of water-baths; Slowly drip the copperas solution 2mL of iron content 0.89mmol/mL, dripping 10wt % sodium-acetate adjusting pH simultaneously is 5, stops to drip continued stirring reaction 1.0 h; Other are with embodiment 1, and gained title complex iron-holder is 15.8 %.
Claims (6)
1. the preparation method of the ferrous title complex of water soluble soybean polysaccharide is characterized in that, may further comprise the steps:
(1) with after water soluble soybean polysaccharide and the catalyst dissolution; Place 40 ~ 90 ℃ of water-baths, stir and drip copperas solution, dripping hydrochloric acid or sodium acetate soln simultaneously down; Keeping the pH of reaction solution is 2 ~ 5, and ferrous sulfate dripped the continued stirring reaction 0.5 ~ 3 hour;
(2) reaction solution cooling back alcohol is analysed, spinning, and the gained deposition gets the ferrous title complex of water soluble soybean polysaccharide after washing, drying;
Said catalyzer is Citrate trianion or tartrate.
2. method according to claim 1 is characterized in that, said Citrate trianion is trisodium citrate or citric acid tri potassium, and said tartrate is sodium tartrate or soluble tartrate.
3. method according to claim 1 is characterized in that, the mass ratio of said water soluble soybean polysaccharide and catalyzer is 0.25:1 ~ 5:1.
4. method according to claim 1 is characterized in that, it is in reaction solution, to add absolute ethyl alcohol that said alcohol is analysed, and separates out deposition.
5. method according to claim 1 is characterized in that, said washing is alcohol to be analysed the gained deposition wash with 95 % ethanol, absolute ethyl alcohol and anhydrous propanone successively.
6. method according to claim 1 is characterized in that, said drying is warm air drying, vacuum-drying, lyophilize or spraying drying.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011101325045A CN102167752B (en) | 2011-05-23 | 2011-05-23 | Preparation method of water-soluble soybean polysaccharide ferrous coordination compound |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011101325045A CN102167752B (en) | 2011-05-23 | 2011-05-23 | Preparation method of water-soluble soybean polysaccharide ferrous coordination compound |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102167752A CN102167752A (en) | 2011-08-31 |
| CN102167752B true CN102167752B (en) | 2012-08-08 |
Family
ID=44489091
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011101325045A Expired - Fee Related CN102167752B (en) | 2011-05-23 | 2011-05-23 | Preparation method of water-soluble soybean polysaccharide ferrous coordination compound |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102167752B (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102511926B (en) * | 2011-12-23 | 2014-04-02 | 华南理工大学 | Method for extracting and separating laminarin with low molecular weight and application thereof in cigarette products |
| CN109824792A (en) * | 2018-12-20 | 2019-05-31 | 中国农业科学院兰州畜牧与兽药研究所 | A kind of preparation method and applications of Radix Salviae Miltiorrhizae ferrous iron polysaccharide |
| CN112094361A (en) * | 2020-11-02 | 2020-12-18 | 石家庄飞龙饲料有限公司 | Agaricus blazei murill polysaccharide iron complex, preparation method and application |
| CN114288320A (en) * | 2021-12-29 | 2022-04-08 | 珠海天翼医药技术开发有限公司 | Oral iron supplement for pigs and preparation method thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1995068A (en) * | 2005-12-31 | 2007-07-11 | 华中科技大学 | Angelica polysaccharide iron and its preparing process |
| CN101003580A (en) * | 2006-01-20 | 2007-07-25 | 青岛科技大学 | Method for preparing polyferose |
| CN101390923A (en) * | 2007-09-21 | 2009-03-25 | 韩泳平 | Preparation method of gadol polyferose composite formaulation and use thereof |
| CN101816697A (en) * | 2009-09-24 | 2010-09-01 | 兰州理工大学 | Method for preparing organic iron supplement by using radix codonopsitis |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070077313A1 (en) * | 2005-10-04 | 2007-04-05 | U.S. Pharmaceutical Corporation | Toleration iron supplement compositions |
-
2011
- 2011-05-23 CN CN2011101325045A patent/CN102167752B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1995068A (en) * | 2005-12-31 | 2007-07-11 | 华中科技大学 | Angelica polysaccharide iron and its preparing process |
| CN101003580A (en) * | 2006-01-20 | 2007-07-25 | 青岛科技大学 | Method for preparing polyferose |
| CN101390923A (en) * | 2007-09-21 | 2009-03-25 | 韩泳平 | Preparation method of gadol polyferose composite formaulation and use thereof |
| CN101816697A (en) * | 2009-09-24 | 2010-09-01 | 兰州理工大学 | Method for preparing organic iron supplement by using radix codonopsitis |
Non-Patent Citations (4)
| Title |
|---|
| 李公斌，王振宇.黑木耳多糖与Fe(Ⅱ)螯合物的制备工艺及红外光谱研究.《食品工业科技》.2006,第27卷(第5期), * |
| 李玉贤等.茶叶多糖铁配合物的合成及一般性质研究.《食品研究与开发》.2005,(第05期), * |
| 秦卫东等.金针菇多糖螯合亚铁的制备.《徐州工程学院学报(自然科学版)》.2009,(第04期), * |
| 陈作伟等.正交试验研究当归多糖铁合成工艺.《西南国防医药》.2003,(第04期), * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102167752A (en) | 2011-08-31 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102167752B (en) | Preparation method of water-soluble soybean polysaccharide ferrous coordination compound | |
| CN105054222B (en) | A kind of bean product natural biological antiseptic antistaling agent and its preparation and application | |
| JP2008525445A5 (en) | ||
| Chohan et al. | Transition metal acetylsalicylates and their anti-inflammatory activity | |
| CN103735659B (en) | Preparation method and use of hairyvein agrimony effective ingredient | |
| CN102351867A (en) | Natural chlorophyll iron calcium salt and preparation method thereof | |
| CN102860512B (en) | Preparation method of black-bone chicken peptide and iron chelated biological iron supplement | |
| CN102584472B (en) | Special fertilizer for producing healthcare agricultural products and preparation method thereof | |
| CN102219864A (en) | Preparation method for water-soluble soybean polysaccharide trivalent ferric complex | |
| CN105995947B (en) | A method of starch zinc complex nutrition fortifier is produced using impulse electric field | |
| CN100558257C (en) | A kind of spray drying process of water-soluble iron chelate of glycine | |
| CN104861016A (en) | Glycyrrhizic acid metal complex preparation method | |
| CN101372354B (en) | Preparation an use of selenium-coated copper chloride hydroxide | |
| CN105924535B (en) | A kind of preparation method of astragalus polyose iron | |
| CN110642959B (en) | Auricularia fuscosuccinea acidic polysaccharide, and preparation method and application thereof | |
| CN102603418A (en) | Composite ferrum preparation for correcting iron-deficient chlorosis of fruit trees and increasing ferrum contents in fruit | |
| CN101766212A (en) | Longan preservative | |
| CN101570554B (en) | Method for preparing zinc supplementing agent carboxymethyl chitosan oligosaccharide zinc | |
| CN103330718A (en) | Preparation method of gynostemma pentaphyllum polysaccharide-iron complex | |
| CN103044571A (en) | Iron dextran bulk drug and preparation method thereof | |
| CN101200747B (en) | Physical fields aided metal-coordination chitosan positioning enzymolysis method | |
| US20060276538A1 (en) | Metal carnitine chelates | |
| CN113563492B (en) | Molecular type low-ester copper pectin and preparation method thereof | |
| MXPA05002523A (en) | Use of metal chelates in human or animal feeding. | |
| CN110922398B (en) | Production method of 2-pyridyl-4-methylthiazole-5-carboxylic acid bisabolone ester spice |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20120808 Termination date: 20150523 |
|
| EXPY | Termination of patent right or utility model |