CN101390923A - Preparation method of gadol polyferose composite formaulation and use thereof - Google Patents

Preparation method of gadol polyferose composite formaulation and use thereof Download PDF

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CN101390923A
CN101390923A CNA2007100500869A CN200710050086A CN101390923A CN 101390923 A CN101390923 A CN 101390923A CN A2007100500869 A CNA2007100500869 A CN A2007100500869A CN 200710050086 A CN200710050086 A CN 200710050086A CN 101390923 A CN101390923 A CN 101390923A
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ethanol
ferrum
radix rhodiolae
polysaccharide
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韩泳平
黄家锟
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Abstract

The invention belongs to the herbal technical field, in particular to the preparation of rhodiola polyferose compound preparation and the application for anti-iron deficiency anemia. The invention adopts rhodiola crenulata medicinal material as the raw material and obtains alcohol extract and polysaccharide fraction through extraction and purification; wherein, polysaccharide has complex reaction with iron ion to produce rhodiola polyferose which is added with alcohol extract to prepare compound preparation; the rhodiola polyferose compound preparation is used for the treatment of iron deficiency anemia; the preparation method of the rhodiola polyferose compound preparation is simpler and easy for industrialization production.

Description

A kind of preparation and application of Radix Rhodiolae polysaccharide ferrum compound preparation
Technical field
The present invention relates to technical field of Chinese medicines, be specifically related to the preparation and the application in resistance to iron deficiency anemia disease thereof of Radix Rhodiolae polysaccharide ferrum compound preparation.
Background technology
Iron deficiency anemia (IDA) is one of the highest deficiency disease of whole world sickness rate.In the world, people's anemia of 1/3 is arranged in 5,000,000,000 populations approximately, and the underfed crowd of IDA or ferrum accounts for 500,000,000, is 4 times of developed country in developing country's iron deficiency (ID) incidence rate, has IDA or ferrum malnutrition at the population of China nearly 20%.
Existing iron-supplementing preparation is divided into two classes usually: a class is an inorganic iron, mainly refers to ferrous sulfate, makes the compound controlled release preparation as the vitamin that adds in ferrous sulfate of CN94108819.7 patent application report; Another kind of is organic ferrum, mainly include machine acid iron complex, sodium iron ethylenediaminetetraacetate (EDTA ferrum) novel formulation as CN02146594.0 patent application report, and the complex of ferrum and aminoacid, protein etc., utilize hydroxy methionine and ferrum as CN00113615.1 patent application report are made chelate as feed additive, the complex that plant or mushroom polysaccharide and ferrum form has also appearred in recent years, as U.S. Patent number is 3,821,192 have openly reported a kind of glucose, maltose, low molecular dextran and FeCl of using 3Reaction forms sugar-iron complex, and and for example the utilize Sargassum polysaccharides and the trace element ion complexation of CN96102440.2 patent application report are reacted into metal complexs such as Sargassum polysaccharides ferrum, can be used for animal feed additive.Comparatively speaking, inorganic chalybeate is simple in structure, preparation is easy, cost is low; Organic chalybeate then easily absorbs, little to the GI irritation effect, be difficult for taking place that appetite lowers, feels sick, gastrointestinal reactions such as vomiting and diarrhoea.As a kind of novel organic ferrum, the polyferose compounds is to be the synthetic organic iron complex of carrier and ferrum with the polysaccharide, and stable in properties can be absorbed at intestinal with molecularity, can give full play to the synergism of polysaccharide and ferrum; Simultaneously, because no free iron ion exists, can obviously reduce the gastrointestinal zest.
Tibetan medicine's medicine Radix Rhodiolae commonly used is the natural plants that is grown among the snowy region of height above sea level 3500-5000m iceberg, Tibet, and according to Four-Volume Medical Code record, this product is used for syndrome of spleen-deficiency, the energy invigorating the spleen and benefiting QI, arrogate to oneself and control deficiency of spleen-QI and decline, diseases such as fatigue and weakness are single with certain curative effect is promptly arranged; This product is used for syndrome of deficiency of blood, and the energy QI invigorating and blood producing can singly be used or use with the hematonic compatibility.Version Pharmacopoeia of the People's Republic of China record in 2005, Radix Rhodiolae has benefiting QI for activating blood circulation, promotes blood circulation and relieving asthma, be used for blood stagnancy due to deficiency of QI, obstruction of qi in the chest and cardialgia, apoplectic hemiplegia, many-sided effects such as asthenia asthma.Recent studies shows, contained rhodioside and tyrosol have resisting fatigue, anti-hypoxia, resisting microwave radiation, antitoxin and to nervous system and metabolic dual regulation in the Radix Rhodiolae, in environmental adaptation, all have a very important role at aspects such as military medicine, aerospace medicine, sports medical science and sanipraticss.Polysaccharide composition wherein also has multiple biological activitys such as immunomodulating, blood sugar lowering, blood fat reducing, antitumor, antiviral as the main effective site of medical material.
This experiment is a raw material with the Radix Rhodiolae medical material, obtain Radix Rhodiolae polysaccharide through extraction, separation, purification, synthetic on this basis Radix Rhodiolae polysaccharide ferrum, cooperate Radix Rhodiolae ethanol extract extractum again, make Radix Rhodiolae polysaccharide ferrum compound preparation, wish by approach such as iron supplement, invigorating the spleen and benefiting QI, QI invigorating and blood producing, immunomodulating treatment iron deficiency anemia.
Summary of the invention:
Problem to be solved by this invention provides a kind of effective site one Radix Rhodiolae polysaccharide ferrum of resistance to iron deficiency anemia, applicable to clinical.
The invention provides a kind of Chinese medicine preparation that contains Radix Rhodiolae polysaccharide ferrum and Radix Rhodiolae ethanol extract extractum, said preparation is the oral solid formulation formed of 10%~33.3% Radix Rhodiolae polysaccharide ferrum, 10%~33.3% Radix Rhodiolae ethanol extract extractum and 80%~33.3% pharmaceutic adjuvant by percentage by weight and is the oral liquid that 1.5% Radix Rhodiolae polysaccharide ferrum, 1.5% Radix Rhodiolae ethanol extract extractum, 20% pharmaceutic adjuvant and water are formed by percentage by weight.
Oral solid formulation of the present invention is: capsule, granule
Oral liquid of the present invention is: oral liquid
Another problem to be solved by this invention is open Radix Rhodiolae polysaccharide ferrum and the above-mentioned preparation method that contains Radix Rhodiolae polysaccharide ferrum Chinese medicine preparation.
Particular content:
The preparation of 1 Radix Rhodiolae extract
Get the Radix Rhodiolae medical material, use alcohol reflux, filter, filtrate decompression is condensed into thick paste, and oven dry obtains ethanol extract then.
Medicinal residues volatilize, and add distilled water, and reflux is filtered, and the concentrating under reduced pressure extracting solution adds ethanol then, separates out precipitation, leaves standstill, and spends the night, and is centrifugal, obtains the rufous insoluble matter, and vacuum drying obtains crude polysaccharides.Crude polysaccharides is redissolved in an amount of distilled water, add papain and carry out enzyme hydrolysis except that albumen, concentrating hydrolysate, precipitate with ethanol, precipitation is dissolved in an amount of distilled water, removes albumen, concentrated extracting solution once more with the sevage method, precipitate with ethanol, centrifugal collecting precipitation is used 95% ethanol, dehydrated alcohol successively, the absolute ether washing, vacuum drying must be made with extra care polysaccharide.
The preparation of 2 Radix Rhodiolae polysaccharide ferrum and general aspects
2.1 dry refining polysaccharide is got in the preparation of Radix Rhodiolae polysaccharide ferrum, trisodium citrate joins in the three-necked bottle, uses suitable dissolved in distilled water.Do not stop to stir in hot bath, slowly drip liquor ferri trichloridi and sodium hydroxide solution simultaneously, the pH value of control solution within the specific limits.When occurring the brownish red infusible precipitate in the reactant liquor, stop to drip ferric chloride and sodium hydroxide solution immediately, continue in hot bath, to stir 1h.After the question response liquid cooling but, centrifugal.Get the dark red brown liquid in upper strata, concentrating under reduced pressure, precipitate with ethanol, use 95% ethanol, dehydrated alcohol, each 2 washing precipitation of absolute ether respectively, vacuum drying gets crude polysaccharides ferrum, and crude polysaccharides ferrum is dissolved in an amount of distilled water, in the bag filter of packing into, respectively with circulating water flushing and each 24h of distill water dialysis, precipitate with ethanol, the centrifuging and taking precipitation, precipitation is respectively washed 2 times with 95% ethanol, dehydrated alcohol, absolute ether successively, vacuum drying gets polyferose (elaboration).2.2 measuring, the Radix Rhodiolae polysaccharide iron content presses Li Yuxian etc. at " Chinese patent medicine " 2006; The content assaying methods of report operation in 28 (5), obtain standard curve and be A=0.2036C+0.0104 (r=0.9996, n=3).Draw that iron content is in the Radix Rhodiolae polysaccharide ferrum: 23.05%.
2.3 the general aspects Radix Rhodiolae polysaccharide ferrum of Radix Rhodiolae polysaccharide ferrum is amorphous chocolate brown powder, nothing is smelt, and is soluble in water, is insoluble to methanol, ethanol, organic reagents such as ether, K 4[Fe (CN 6)] do not contain free F in the evidence Radix Rhodiolae polysaccharide molten iron solution 3+, illustrate to have generated comparatively stable coordination compound.
2.4 Radix Rhodiolae polysaccharide iron oxide red external spectrum figure it is reported that infrared spectrum can be differentiated the type of FeOOH nuclear in polyferose (III) coordination compound.We have done the infrared spectrum (the results are shown in Figure 1) of Radix Rhodiolae polysaccharide ferrum for this reason.As shown in Figure 1, it is at 850cm -1With 680cm -1Characteristic absorption and Marshall PR are at J Colloid Interlace Sci, and the infrared spectrum of 1971 (37) β-FeOOH that reported is consistent, and ferrum nuclear is β-FeOOH nuclear among prompting Fig. 1.
2.5 the preparation of Radix Rhodiolae polysaccharide iron preparation
2.5.1 the preparation Radix Rhodiolae ethanol extract of capsule, polyferose, medical starch mix homogeneously sieve, and remove impurity, and bulky grain is pulverized the back after sieve with pulverizer.Fill, granulate, packing.
2.5.2 the preparation Radix Rhodiolae ethanol extract of granule, polyferose, carboxymethyl starch sodium, the micropowder cellulose, the sucrose mix homogeneously is made soft material with starch slurry, sieves, oven dry, the granulate that sieves, drying is all by a sieve and No. four sieves.Pack stored dry.
2.5.3 the preparation of oral liquid is water-soluble with equivalent Radix Rhodiolae ethanol extract extractum and Radix Rhodiolae polysaccharide ferrum, adds the sucrose slurry and does correctives, adds benzoic acid as antiseptic, stirs bottling, sterilization.
3 pharmacodynamic experiments
3.1 material will be dissolved in 200ml water by embodiment 1 polyferose 2.108g that obtains and the Radix Rhodiolae ethanol extract extractum 2.108g mix homogeneously that obtains by embodiment 2, (iron content is 2.43mgml to make compound preparation -1).Positive control adopts the ferrous oral administration solution of compound three-vitamines, the ferrous 120mg of every 10ml sulfur acid, Chengdu medicine company limited product, lot number H51023375.Calculate the experimental animal dosage in the body surface area ratio.
3.2 80 of laboratory animal Kunming mouses, 18~22g, male and female half and half are provided by Sichuan University's West China Experimental Animal Center.3.3 the general animal feed of feedstuff is provided by Sichuan University's West China Experimental Animal Center.Low ferrum feedstuff is by low ferrum feed formula preparation, and wherein sweet potato starch 54%, whole milk powder 40%, Oleum Glycines 5%, Sal 1%.
3.4 instrument BECKMAN ATC differential blood count instrument, the UV-2201 ultraviolet-uisible spectrophotometer.
3.5 getting 78 mices, method is divided into blank group, compound preparation low dose group (20mgkg at random -1), middle dosage group (40mgkg -1), high dose group (60mgkg -1), positive group (39.78mgkg -1) and model group.The administration of dividing into groups as stated above except that the blank group, (blank group is pressed 0.2ml10g to gastric infusion 20d continuously -1Irritate normal saline), blank group is fed conventional feed, drinking public water supply, all the other each groups are fed low ferrum feedstuff, drink deionized water.Except that the blank group, the auxiliary blood-letting of all the other each groups, 5d once, each eye socket venous plexus blood-letting 0.5ml that adopts, blood-letting is 4 times altogether, weighed in the 20th day, pluck eyeball and get blood, adopt BECKMAN ATC differential blood count instrument to survey hemoglobin (Hb), erythrocyte (RBC), separation of serum (SI), press " modern clinical biochemical ecsomatics " (People's Medical Officer Press of chief editors such as Zhang Xiuming, 2001) the chrome azurol S method of report in adopts the UV-2201 ultraviolet-uisible spectrophotometer to survey serum levels of iron content, dissects mice and claims spleen heavy, calculate the spleen coefficient, the result adopts the t method of inspection to analyze.
3.6 result of the test
Table 1 Radix Rhodiolae polysaccharide ferrum compound preparation is to the influence of iron deficiency anemia mice Hb, RBC (x ± s)
Compare with model group *P<0.01
By table 1 as seen, model group mice Hb, RBC be 84.677 ± 11.790g/L respectively, and 5.2177 ± 0.657 * 10 12/ L is with blank group 140.031 ± 9.5364g/L, 8.2438 ± 0.454 * 10 12Symptoms such as/L relatively has utmost point significant difference, and lethargy appears in animal simultaneously, and fur is fluffy, inactive prove the modeling success.
The administration group is irritated stomach Radix Rhodiolae polysaccharide ferrum compound preparation every day in modeling, through 20 days treatment, the Hb of mice, RBC value were compared with model group near blank group mice, and utmost point significant difference is arranged.
Table 2 Radix Rhodiolae polysaccharide ferrum compound preparation is to the influence of iron deficiency anemia mice body weight (x ± s)
Figure A200710050086D00062
Compare with model group *P<0.05, *P<0.01, the body weight of mice when t1 begins for experiment, the body weight of mice when t2 finishes for experiment.
As shown in Table 2, through the experiment of 20d, each is organized the mice body weight and all raises, and blank group, middle dosage group, low dose group, positive group mice body weight are higher than model group, its empty group mice body weight and model group relatively have utmost point significant difference, and relatively there were significant differences for middle dosage group mice body weight and model group.Three experimental grouies, positive group, blank group mice body weight are rised in value all greater than model group, and wherein, the increment of blank group mice body weight relatively has utmost point significant difference with model group, and middle dosage group, low dose group mice body weight are rised in value and relatively there were significant differences for model group.
Table 3 Radix Rhodiolae polysaccharide ferrum compound preparation is to the influence of iron deficiency anemia mice serum ferrum, spleen coefficient (x ± s)
Figure A200710050086D00071
Compare with model group *P<0.05, *P<0.01
As shown in Table 3, the serum levels of iron content of experimental group, positive group, blank group mice all is higher than model group, wherein serum levels of iron content and the model group of high dose group, middle dosage group, positive group and blank group mice relatively have utmost point significant difference, and relatively there were significant differences for serum levels of iron content of low dose group mice and model group.The spleen of experimental group, positive group, blank group mice heavily reaches the spleen coefficient and all is higher than model group, wherein, in the spleen of dosage group, low dose group and blank group mice overweight blank group relatively there were significant differences, spleen coefficient and the model group of high dose group, middle dosage group, low dose group and blank group mice relatively have utmost point significant difference, and relatively there were significant differences for spleen coefficient of positive group mice and model group.
Description of drawings
Fig. 1 is the infrared spectrogram of Radix Rhodiolae polysaccharide ferrum
Specific embodiments
The preparation of embodiment 1 Radix Rhodiolae polysaccharide extract
Get Radix Rhodiolae medical material 2000g,, filter at 75 ℃ of following reflux, extract, 3h with 3 times of amount 80% ethanol, filtrate decompression is condensed into thick paste, and oven dry obtains ethanol extract extractum 520g then.
Medicinal residues volatilize (1466g), add 12,10,8 times of amount distilled water respectively, 80 ℃ of following reflux, extract, 3 times, each 2h filters, and concentrating under reduced pressure filtrate is to viscous liquid, adding 95% ethanol to solution determining alcohol then is 80%, separate out precipitation, leave standstill, spend the night, centrifugal, obtain the rufous insoluble matter, 40 ℃ of following vacuum dryings obtain crude polysaccharides 480g.Crude polysaccharides is redissolved in the 5000ml distilled water, ratio according to bulking value concentration 1% adds papain (w/v), at pH6.4, hydrolysis 3h under 60 ℃ of conditions, after hydrolyzed solution is evaporated to 1/5, add equal-volume 95% ethanol precipitate with ethanol again, precipitation oven dry back adds the 2500ml distilled water makes its dissolving, remove albumen 2 times with the Sevage method, be to add equal-volume chloroform-n-butyl alcohol mixed solution (4: 1) in the polysaccharide solution, vibration 30min is centrifugal, get upper solution, be concentrated into 1/5, add 3 times of amount 95% ethanol precipitate with ethanol, centrifugal collecting precipitation, use 95% ethanol successively, dehydrated alcohol, the absolute ether washing, vacuum drying must be made with extra care polysaccharide 102.6g.
The preparation of embodiment 2 Radix Rhodiolae polysaccharide ferrum
Get dry refining polysaccharide 2g, trisodium citrate 0.5g and join in the 250ml three-necked bottle, use the 100ml dissolved in distilled water.Do not stop to stir under 70 ℃, drip liquor ferri trichloridi (2mol/L) and 20%NaOH solution simultaneously, the pH value of control solution is 8.0~8.5.When occurring the brownish red infusible precipitate in the reactant liquor, stop to drip ferric chloride and sodium hydroxide solution, continue in hot bath, to stir 1h.After the question response liquid cooling but, centrifugal.Get the dark red brown liquid in upper strata, be evaporated to original volume 1/3, adding 95% ethanol to solution determining alcohol is 80%, standing over night, centrifugal collecting precipitation, use 95% ethanol, dehydrated alcohol, each 2 washing precipitation of absolute ether respectively, 40~50 ℃ of vacuum dryings get crude polysaccharides ferrum, and crude polysaccharides ferrum is dissolved in an amount of distilled water, in the bag filter of packing into, respectively with circulating water flushing and each 24h of distill water dialysis, precipitate with ethanol, the centrifuging and taking precipitation, precipitation is respectively washed 2 times with 95% ethanol, dehydrated alcohol, absolute ether successively, 40~50 ℃ of vacuum dryings get polyferose 0.56g.
The preparation of embodiment 3 capsules
The Radix Rhodiolae ethanol extract extractum that will obtain by embodiment 1, by polyferose, each 100g of medical starch that embodiment 2 obtains, mix homogeneously, sieve (40 order) removes impurity, and bulky grain is pulverized the back after sieve with pulverizer.Fill with No. 2 capsules then, make 1000 altogether, every 0.285g, control moisture is within 9.0%, and plastic seal is packed.Three times on the oral one, one time three.The preparation of embodiment 4 granules
The Radix Rhodiolae ethanol extract extractum that 100g obtains by embodiment 1, the polyferose that 100g obtains by embodiment 2,200g carboxymethyl starch sodium (crossing 100 mesh sieves), 350g micropowder cellulose (crossing 100 mesh sieves), 50g sucrose (is selected white sugar for use, was descended pulverizing with preceding dry 1~2 hour in 60 ℃, cross the Icing Sugar of No. four sieves, hermetically storing) mix homogeneously is made soft material with the 200g starch slurry, crosses 20 mesh sieves, oven dry, cross 20 mesh sieve granulate, drying, control moisture is in 2%, all, screen out fine particle and fine powder after No. four by a sieve.Pack, every bag of 10g packs, stored dry.Oral one day 10g.
The preparation of embodiment 5 oral liquids
The Radix Rhodiolae ethanol extract extractum that 15g is obtained by embodiment 1,15g mixes by the Radix Rhodiolae polysaccharide ferrum that embodiment 2 obtains, be dissolved in 500ml water, other gets sucrose 200g and makes syrup, merges with above-mentioned medicinal liquid, add the 0.5ml benzoic acid and make antiseptic, adjust weight to 1000ml, stir, filter, bottling, sterilization.Three times on the oral one, a 20ml.

Claims (3)

1. the preparation and the application of a Radix Rhodiolae polysaccharide ferrum compound preparation, the content that it is characterized in that ferrum in the polyferose is 23.05%, ferrum nuclear exists with β-FeOOH form.
2. method for preparing the described Radix Rhodiolae polysaccharide ferrum of claim 1 compound preparation is characterized in that:
1. get the Radix Rhodiolae medical material,, filter at 75 ℃ of following reflux, extract, 3h with 3 times of amount 80% ethanol, filtrate decompression is condensed into thick paste, and oven dry obtains ethanol extract extractum.
2. medicinal residues volatilize, and add 12,10,8 times of amount distilled water respectively, 80 ℃ of following reflux, extract, 3 times, each 2h filters, and the concentrating under reduced pressure extracting solution is to viscous liquid, adding 95% ethanol to solution determining alcohol then is 80%, separate out precipitation, leave standstill, spend the night, centrifugal, obtain the rufous insoluble matter, 40 ℃ of following vacuum dryings obtain crude polysaccharides.Crude polysaccharides is redissolved in distilled water, ratio according to bulking value concentration 1% adds papain (w/v), at pH6.4, enzyme hydrolysis 3h under 60 ℃ of conditions, behind the concentrating hydrolysate to 1/5, add equal-volume 95% ethanol precipitate with ethanol again, precipitation oven dry back adds distilled water makes its dissolving, remove albumen 2 times with the Sevage method, be to add equal-volume chloroform-n-butyl alcohol mixed solution (4:1) in the polysaccharide solution, vibration 30min is centrifugal, get upper solution, be concentrated into 1/5, precipitate with ethanol, centrifugal collecting precipitation, use 95% ethanol successively, dehydrated alcohol, the absolute ether washing, vacuum drying must be made with extra care polysaccharide.
3. get dry refining polysaccharide 2g, trisodium citrate 0.5g in proportion and join in the 250ml three-necked bottle, use the 100ml dissolved in distilled water.Do not stop to stir under 70 ℃, slowly drip liquor ferri trichloridi (2mol/L) and 20% NaOH solution simultaneously, the pH value of control solution is 8.0~8.5.When occurring the brownish red infusible precipitate in the reactant liquor, stop to drip ferric chloride and sodium hydroxide solution, continue in hot bath, to stir 1h.After the question response liquid cooling but, centrifugal.Get the dark red brown liquid in upper strata, be evaporated to original volume 1/3, adding 95% ethanol to solution determining alcohol is 80%, standing over night, centrifugal collecting precipitation, use 95% ethanol, dehydrated alcohol, each 2 washing precipitation of absolute ether respectively, 40~50 ℃ of vacuum dryings get crude polysaccharides ferrum, and crude polysaccharides ferrum is dissolved in an amount of distilled water, in the bag filter of packing into, respectively with circulating water flushing and each 24h of distill water dialysis, precipitate with ethanol, the centrifuging and taking precipitation, precipitation is respectively washed 2 times with 95% ethanol, dehydrated alcohol, absolute ether successively, 40~50 ℃ of vacuum dryings get polyferose.
4. with percentage by weight be 10%~33.3% set by step 3. to Radix Rhodiolae polysaccharide ferrum, 10%~33.3% set by step 1. Radix Rhodiolae ethanol extract extractum, 80%~33.3% pharmaceutic adjuvant be mixed and made into oral solid formulation.
5. with percentage by weight be 1.5% set by step 3. to Radix Rhodiolae polysaccharide ferrum, 1.5% the Radix Rhodiolae ethanol extract extractum, 20% pharmaceutic adjuvant and the water that 1. obtain set by step be mixed and made into oral liquid.
3. the purposes by the described Radix Rhodiolae polysaccharide ferrum of claim 1 compound preparation is characterized in that: as the medicine of treatment iron deficiency anemia disease.
CNA2007100500869A 2007-09-21 2007-09-21 Preparation method of gadol polyferose composite formaulation and use thereof Pending CN101390923A (en)

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Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102086232A (en) * 2010-12-15 2011-06-08 青岛市中心医院 Method for preparing polyferose
CN102167752A (en) * 2011-05-23 2011-08-31 华南理工大学 Preparation method of water-soluble soybean polysaccharide ferrous coordination compound
CN103224571A (en) * 2013-04-08 2013-07-31 江苏久吾高科技股份有限公司 Method for purifying polysaccharide iron complex based on ultrafiltration membrane method
CN101589064B (en) * 2007-01-19 2014-01-29 维福(国际)股份公司 Iron-hydrocarbon complex compounds
CN104177505A (en) * 2013-05-24 2014-12-03 重庆圣华曦药业股份有限公司 Polysaccharide iron complex separating and purifying method
CN105497059A (en) * 2016-01-25 2016-04-20 南京耕丰医药科技有限公司 Compound of caulis dendrobii extract and iron and preparing method and application of compound
CN111333745A (en) * 2020-04-17 2020-06-26 内江师范学院 Citrus peel polysaccharide iron (III) compound and preparation method thereof

Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101589064B (en) * 2007-01-19 2014-01-29 维福(国际)股份公司 Iron-hydrocarbon complex compounds
CN102086232A (en) * 2010-12-15 2011-06-08 青岛市中心医院 Method for preparing polyferose
CN102167752A (en) * 2011-05-23 2011-08-31 华南理工大学 Preparation method of water-soluble soybean polysaccharide ferrous coordination compound
CN102167752B (en) * 2011-05-23 2012-08-08 华南理工大学 Preparation method of water-soluble soybean polysaccharide ferrous coordination compound
CN103224571A (en) * 2013-04-08 2013-07-31 江苏久吾高科技股份有限公司 Method for purifying polysaccharide iron complex based on ultrafiltration membrane method
CN103224571B (en) * 2013-04-08 2015-04-29 江苏久吾高科技股份有限公司 Method for purifying polysaccharide iron complex based on ultrafiltration membrane method
CN104177505A (en) * 2013-05-24 2014-12-03 重庆圣华曦药业股份有限公司 Polysaccharide iron complex separating and purifying method
CN104177505B (en) * 2013-05-24 2016-10-19 重庆圣华曦药业股份有限公司 A kind of method separated with purified polysaccharide iron complexes
CN105497059A (en) * 2016-01-25 2016-04-20 南京耕丰医药科技有限公司 Compound of caulis dendrobii extract and iron and preparing method and application of compound
CN105497059B (en) * 2016-01-25 2019-04-09 南京耕丰医药科技有限公司 A kind of Herba Dendrobii extract and the compound of iron and the preparation method and application thereof
CN111333745A (en) * 2020-04-17 2020-06-26 内江师范学院 Citrus peel polysaccharide iron (III) compound and preparation method thereof

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