CN112245527A - Preparation method of active polysaccharide extract, functional beverage and effervescent granule with homology of medicine and food - Google Patents
Preparation method of active polysaccharide extract, functional beverage and effervescent granule with homology of medicine and food Download PDFInfo
- Publication number
- CN112245527A CN112245527A CN202011253839.8A CN202011253839A CN112245527A CN 112245527 A CN112245527 A CN 112245527A CN 202011253839 A CN202011253839 A CN 202011253839A CN 112245527 A CN112245527 A CN 112245527A
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- Prior art keywords
- active polysaccharide
- parts
- preparation
- medicine
- active
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Links
- 150000004676 glycans Chemical class 0.000 title claims abstract description 99
- 229920001282 polysaccharide Polymers 0.000 title claims abstract description 99
- 239000005017 polysaccharide Substances 0.000 title claims abstract description 99
- 239000008187 granular material Substances 0.000 title claims abstract description 51
- 239000003814 drug Substances 0.000 title claims abstract description 31
- 235000020510 functional beverage Nutrition 0.000 title claims abstract description 28
- 238000002360 preparation method Methods 0.000 title claims abstract description 20
- 239000000284 extract Substances 0.000 title claims abstract description 15
- 235000013305 food Nutrition 0.000 title claims abstract description 11
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 34
- 238000001704 evaporation Methods 0.000 claims abstract description 11
- 239000002994 raw material Substances 0.000 claims abstract description 11
- 241000205585 Aquilegia canadensis Species 0.000 claims abstract description 7
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- 235000006200 Glycyrrhiza glabra Nutrition 0.000 claims abstract description 7
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- LPLVUJXQOOQHMX-QWBHMCJMSA-N glycyrrhizinic acid Chemical compound O([C@@H]1[C@@H](O)[C@H](O)[C@H](O[C@@H]1O[C@@H]1C([C@H]2[C@]([C@@H]3[C@@]([C@@]4(CC[C@@]5(C)CC[C@@](C)(C[C@H]5C4=CC3=O)C(O)=O)C)(C)CC2)(C)CC1)(C)C)C(O)=O)[C@@H]1O[C@H](C(O)=O)[C@@H](O)[C@H](O)[C@H]1O LPLVUJXQOOQHMX-QWBHMCJMSA-N 0.000 claims abstract description 7
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- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 24
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- DBTMGCOVALSLOR-DEVYUCJPSA-N (2s,3r,4s,5r,6r)-4-[(2s,3r,4s,5r,6r)-3,5-dihydroxy-6-(hydroxymethyl)-4-[(2s,3r,4s,5s,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxyoxan-2-yl]oxy-6-(hydroxymethyl)oxane-2,3,5-triol Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](CO)O[C@H](O)[C@@H]2O)O)O[C@H](CO)[C@H]1O DBTMGCOVALSLOR-DEVYUCJPSA-N 0.000 description 1
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- 206010035664 Pneumonia Diseases 0.000 description 1
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- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
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- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
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Classifications
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Landscapes
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Abstract
The invention relates to the technical field of active polysaccharide, in particular to a preparation method of active polysaccharide extract, functional beverage and effervescent granules with homology of medicine and food. The active polysaccharide extracting solution is prepared by selecting 5-15 parts of astragalus, 5-15 parts of rhizoma polygonati, 5-15 parts of poria cocos, 2-10 parts of honeysuckle, 5-15 parts of wolfberry fruit and 0.5-2.5 parts of liquorice as raw materials according to the compatibility of traditional Chinese medicines, and carrying out soaking, decoction, heating extraction, concentration, alcohol precipitation and reduced pressure evaporation on the raw materials. The extraction and preparation process is simpler, the purification steps are reduced, the extraction efficiency is high, the polysaccharide functional components are better reserved, the multifunctional beverage and effervescent granules prepared by adopting the extracting solution have the effect of obviously improving the immunity, and the use and the carrying are very convenient.
Description
Technical Field
The invention relates to the technical field of active polysaccharide, in particular to a preparation method of active polysaccharide extract, functional beverage and effervescent granules with homology of medicine and food.
Background
The active polysaccharide refers to a polysaccharide compound with a certain special physiological activity, and has the function of bidirectionally regulating the physiological rhythm of a human body. Typical active polysaccharides include ganoderan, lycium barbarum polysaccharide, auricularia auricula polysaccharide, laminarin, pine pollen polysaccharide and the like, and have the effects of improving the immunity of organisms, resisting tumors and viruses, reducing blood fat, improving the gastrointestinal health and the like.
The extraction of active polysaccharide refers to that active polysaccharide in natural products is dissolved or released outside cells by using a certain principle and method. The method for extracting polysaccharide at home and abroad mainly comprises a water extraction and alcohol precipitation method, an acid/alkali extraction method, an enzymolysis method, an ultrasonic extraction method, a microwave extraction method and the like. Although the water extraction and alcohol precipitation method is simple in experimental operation and can save cost, the polysaccharide extraction rate is low, and the extracted polysaccharide has more impurities, so that the subsequent operation procedure is complicated; although the extraction rate of the alkali extraction method is slightly higher than that of the water extraction and alcohol precipitation method, only alkali-soluble polysaccharide can be extracted, the extraction range is small, and the impurities are more; the acid extraction method and the alkali extraction method have similar advantages and disadvantages, and are different in that the acid has obvious damage to polysaccharide polymers, the extracted polysaccharide has low viscosity, acid hydrolysis easily generates acid waste liquid, and the extraction cost of the whole polysaccharide is increased in the process of treating wastewater and waste; the polysaccharide extracted by ultrasonic wave and microwave-assisted extraction methods can promote the decomposition of the polysaccharide due to long-time ultrasonic wave radiation, and simultaneously, the temperature of a system is increased due to microwave radiation, so that the polysaccharide structure can be damaged, and the polysaccharide content is reduced; further, the ultrasound effective region is formed in a ring shape under the restriction of the attenuation factor of the ultrasonic wave, and if the diameter of the extraction tank is too large, an ultrasonic blank region is formed in the peripheral wall of the tank.
People now become aware of the importance of health and begin to re-consider the importance of health foods in disease prevention. Particularly, after epidemic outbreak of new coronary pneumonia, people are deeply aware of the importance of human immunity. At present, the types of health care products on the market are many, but most of the health care products are mainly health care products for supplementing various vitamins and minerals in the body and less polysaccharides, and the dosage forms are also oral liquid, capsules and the like. In addition, pure Chinese herbal medicine compatible extracts are rarely used as health care products for improving immunity in the market, and most of the health care products are prepared by extracting a certain specified component from some raw materials and mixing the components with each other. The extraction method has low extraction rate, and the purification steps are complicated, so the energy consumption is high, and the pursuit of people for 'pure natural' products is not met.
In summary, there is a need to improve the existing active polysaccharide extraction process to solve the problems of low extraction rate, poor economy, complicated extraction steps, etc., and develop an active polysaccharide functional health food which uses pure Chinese herbal medicine compatible extracts as main ingredients and has good taste and convenient use, so as to better meet the market demand.
Disclosure of Invention
The invention aims to provide a preparation method of medicinal and edible active polysaccharide extract with simple process and high extraction efficiency aiming at the defects in the prior art.
The second purpose of the present invention is to provide an active polysaccharide functional beverage with convenient use and immunity-improving efficacy and a preparation method thereof, aiming at the defects in the prior art.
The invention also aims to provide the active polysaccharide functional effervescent granule which is convenient to use and has the effect of improving immunity and the preparation method thereof, aiming at the defects in the prior art.
The purpose of the invention is realized by the following technical scheme:
provides a preparation method of active polysaccharide extract with homology of medicine and food, which comprises the following steps:
(1) weighing 5-15 parts of astragalus membranaceus, 5-15 parts of rhizoma polygonati, 5-15 parts of poria cocos, 2-10 parts of honeysuckle, 5-15 parts of wolfberry and 0.5-2.5 parts of liquorice, and adding 10 times of water to soak for a period of time;
(2) boiling the soaked liquid medicine at 100 deg.C, adjusting to 80 deg.C, keeping slightly boiling, decocting for the first time, adding 6 times of water into the separated residue, and repeatedly decocting for the second time;
(3) pouring out the liquid medicine decocted twice, and obtaining supernatant after centrifugal separation;
(4) heating the supernatant to 100 ℃ to boil the extracting solution, then cooling to 85 ℃ to keep slight boiling, cooling to room temperature, adding 95% ethanol until the ethanol content is 50%, sealing and standing overnight;
(5) centrifuging the precipitated medicinal liquid again, evaporating the supernatant under reduced pressure, and removing ethanol to obtain the active polysaccharide extractive solution.
In the technical scheme, in the step (1), the soaking time is 30-40 min.
In the technical scheme, in the step (2), the time for primary decoction is 1h, and the time for secondary decoction is 40 min.
In the technical scheme, in the step (3), the rotation speed of the centrifugation is 4000r/min, and the centrifugation time is 5 min.
In the technical scheme, in the step (5), a rotary evaporator is adopted for reduced pressure evaporation under the conditions of 0.06MPa, 60 ℃ and 1 h.
The invention also provides an active polysaccharide functional beverage, which is prepared from the following raw materials in parts by weight:
the active polysaccharide extracting solution is prepared by the preparation method of the active polysaccharide extracting solution.
Preferably, the concentrated fruit juice is concentrated grapefruit juice, concentrated peach plus orange juice or concentrated blackcurrant juice.
The invention also provides active polysaccharide effervescent granules, which are prepared from the following raw materials in parts by weight:
the sugar powder is prepared by mixing sucrose powder and xylitol according to the mass ratio of 1: 3;
the active polysaccharide extracting solution is prepared by the preparation method of the active polysaccharide extracting solution.
The invention also provides a preparation method of the active polysaccharide effervescent granule, which is characterized by comprising the following steps: the method comprises the following steps:
step a, mixing powdered sugar, citric acid and sodium bicarbonate into auxiliary materials according to a proportion, then slowly spraying the active polysaccharide extracting solution into the mixed auxiliary materials by using a spray bottle on a clean workbench at normal temperature and normal pressure, and stirring while spraying to prepare a soft material;
b, feeding the soft material into a swing type granulator, processing into granules, sieving, drying at 60 ℃, turning over the soft material every 10min in the drying process, and drying to obtain dry granules;
and c, feeding the dry granules into a swing type granulator again for granule stabilization, and sieving to obtain the active polysaccharide effervescent granules.
Preferably, the sieving in step b is 12 mesh sieving and the sieving in step c is 14 mesh sieving.
The invention has the beneficial effects that:
compared with the prior art, the invention has the following advantages:
(1) the extraction process adopts a high-efficiency concentration extraction method to extract main functional components in the raw materials, and compared with the existing health-care products taking Chinese herbal medicines as the raw materials in the market, the extraction and preparation process is simpler, and more polysaccharide functional components can be better reserved due to the reduction of purification steps;
(2) in the aspect of raw materials, the traditional Chinese medicine composition is matched according to the compatibility rule of traditional Chinese medicines, 5-15 parts of astragalus membranaceus, 5-15 parts of rhizoma polygonati, 5-15 parts of poria cocos, 2-10 parts of honeysuckle, 5-15 parts of wolfberry fruits and 0.5-2.5 parts of liquorice in the formula can suppress the side effect of another component, so that the main effect of the traditional Chinese medicine composition is improved, the traditional Chinese medicine composition has the complementary effect, and the effect of improving the immunity of the traditional Chinese medicine composition is more fully exerted;
(3) compared with the traditional granules, the effervescent granules prepared by the invention are more easily welcomed by the relatives in taste and visual sense, the raw materials are medicinal materials in a formula with homology of medicine and food and a Chinese medicinal material list which can be used for health care products, the effervescent granules are safe and reliable, the side effect on human bodies is not needed to worry about, the effervescent granules are suitable for both the old and the young, only a plurality of bags are needed to be put in the bags when people go out for traveling, the occupied space is not occupied, and the effervescent granules are very convenient to use.
Detailed Description
The present invention will be described in further detail with reference to the following examples, but the present invention is not limited thereto.
Example 1: and (3) preparing an active polysaccharide extracting solution.
Weighing 9g of astragalus membranaceus, 9g of rhizoma polygonati, 9g of poria cocos, 6g of honeysuckle, 6g of wolfberry fruits and 1.5g of liquorice, and adding 10 times of water to soak for 30-40 min;
boiling the soaked liquid medicine at 100 ℃, adjusting the temperature to 80 ℃ to keep slightly boiling, carrying out primary decoction for 1h, adding 6 times of water into separated medicine residues, and repeatedly carrying out secondary decoction for 40 min;
pouring out the liquid medicine decocted twice, and centrifuging for 5min at 4000r/min to obtain supernatant;
heating the supernatant to 100 ℃ to boil the extracting solution, then cooling to 85 ℃ to keep slight boiling, cooling to room temperature, adding 95% ethanol until the ethanol content is 50%, sealing and standing overnight;
and (5) centrifuging the precipitated liquid medicine again, and evaporating the supernatant by using a rotary evaporator under reduced pressure to remove ethanol, wherein the evaporation condition is 0.06MPa, 60 ℃ and 1h, so as to obtain the active polysaccharide extracting solution.
Example 2: and (3) preparing an active polysaccharide extracting solution.
Weighing 5g of astragalus membranaceus, 5g of rhizoma polygonati, 5g of poria cocos, 2g of honeysuckle, 5g of wolfberry fruits and 0.5g of liquorice, and adding 10 times of water to soak for 30-40 min;
boiling the soaked liquid medicine at 100 ℃, adjusting the temperature to 80 ℃ to keep slightly boiling, carrying out primary decoction for 1h, adding 6 times of water into separated medicine residues, and repeatedly carrying out secondary decoction for 40 min;
pouring out the liquid medicine decocted twice, and centrifuging for 5min at 4000r/min to obtain supernatant;
heating the supernatant to 100 ℃ to boil the extracting solution, then cooling to 85 ℃ to keep slight boiling, cooling to room temperature, adding 95% ethanol until the ethanol content is 50%, sealing and standing overnight;
and (5) centrifuging the precipitated liquid medicine again, and evaporating the supernatant by using a rotary evaporator under reduced pressure to remove ethanol, wherein the evaporation condition is 0.06MPa, 60 ℃ and 1h, so as to obtain the active polysaccharide extracting solution.
Example 3: and (3) preparing an active polysaccharide extracting solution.
Weighing 15g of astragalus membranaceus, 15g of yellow, 15g of poria cocos, 10g of honeysuckle, 15g of wolfberry and 2.5g of liquorice, and adding 10 times of water to soak for 30-40 min;
boiling the soaked liquid medicine at 100 ℃, adjusting the temperature to 80 ℃ to keep slightly boiling, carrying out primary decoction for 1h, adding 6 times of water into separated medicine residues, and repeatedly carrying out secondary decoction for 40 min;
pouring out the liquid medicine decocted twice, and centrifuging for 5min at 4000r/min to obtain supernatant;
heating the supernatant to 100 ℃ to boil the extracting solution, then cooling to 85 ℃ to keep slight boiling, cooling to room temperature, adding 95% ethanol until the ethanol content is 50%, sealing and standing overnight;
and (5) centrifuging the precipitated liquid medicine again, and evaporating the supernatant by using a rotary evaporator under reduced pressure to remove ethanol, wherein the evaporation condition is 0.06MPa, 60 ℃ and 1h, so as to obtain the active polysaccharide extracting solution.
Example 4: preparing functional beverage containing active polysaccharide.
1. Grapefruit flavored active polysaccharide functional beverages:
weighing 90.8g of grapefruit juice, 77.6g of sucrose syrup and 4g of citric acid, uniformly mixing to prepare concentrated grapefruit juice, then adding 112.5ml of the active polysaccharide extracting solution of the example 1 and 1000ml of purified water, and uniformly mixing to obtain the grapefruit-flavored active polysaccharide functional beverage.
2. Peach and orange flavored active polysaccharide functional beverage
Weighing 40.4g of peach juice, 40.4g of orange juice, 81.8g of cane sugar pulp and 4.5g of citric acid, uniformly mixing to prepare concentrated peach and orange juice, then adding 124ml of the active polysaccharide extracting solution and 1000ml of purified water in the example 1, and uniformly mixing to obtain the active polysaccharide functional beverage with the taste of peach and orange.
3. Blackcurrant-flavored active polysaccharide functional beverage
Weighing 80.2g of blackcurrant juice, 70.8g of cane sugar pulp and 3.5g of citric acid, uniformly mixing to prepare concentrated blackcurrant juice, then adding 100ml of the active polysaccharide extracting solution of the example 1 and 1000ml of purified water, and uniformly mixing to obtain the blackcurrant-flavored active polysaccharide functional beverage.
Example 5: preparing active polysaccharide effervescent granules.
Step a, mixing 240g of powdered sugar, 9g of citric acid and 6g of sodium bicarbonate into auxiliary materials according to a proportion, then slowly spraying 90ml of the polysaccharide extracting solution of the embodiment 1 into the mixed auxiliary materials by using a spray bottle on a clean workbench at normal temperature and normal pressure (25 ℃,101KPa), and stirring while spraying to prepare a soft material;
b, feeding the soft material into a swing type granulator, processing the soft material into granules, then sieving the granules with a 12-mesh sieve, drying the granules at 60 ℃, turning the soft material over every 10min in the drying process, and drying the granules to obtain dry granules;
and c, feeding the dry granules into a swing type granulator again for granulation, and sieving by a 14-mesh sieve to obtain the active polysaccharide effervescent granules.
In the embodiment, the powdered sugar is prepared by mixing sucrose powder and xylitol according to the mass ratio of 1: 3.
Example 6: preparing active polysaccharide effervescent granules.
Step a, mixing 264g of powdered sugar, 8g of citric acid and 6.8g of sodium bicarbonate into auxiliary materials according to a proportion, then slowly spraying 100ml of the polysaccharide extracting solution of the embodiment 1 into the mixed auxiliary materials by using a spray bottle on a clean workbench at normal temperature and normal pressure (25 ℃,101KPa), and stirring while spraying to prepare a soft material;
b, feeding the soft material into a swing type granulator, processing the soft material into granules, then sieving the granules with a 12-mesh sieve, drying the granules at 60 ℃, turning the soft material over every 10min in the drying process, and drying the granules to obtain dry granules;
and c, feeding the dry granules into a swing type granulator again for granulation, and sieving by a 14-mesh sieve to obtain the active polysaccharide effervescent granules.
In the embodiment, the powdered sugar is prepared by mixing sucrose powder and xylitol according to the mass ratio of 1: 3.
Example 7: preparing active polysaccharide effervescent granules.
Step a, mixing 216g of powdered sugar, 10g of citric acid and 5.2g of sodium bicarbonate into auxiliary materials according to a proportion, then slowly spraying 80ml of the polysaccharide extracting solution of the embodiment 1 into the mixed auxiliary materials by using a spray bottle on a clean workbench at normal temperature and normal pressure (25 ℃,101KPa), and stirring while spraying to prepare a soft material;
b, feeding the soft material into a swing type granulator, processing the soft material into granules, then sieving the granules with a 12-mesh sieve, drying the granules at 60 ℃, turning the soft material over every 10min in the drying process, and drying the granules to obtain dry granules;
and c, feeding the dry granules into a swing type granulator again for granulation, and sieving by a 14-mesh sieve to obtain the active polysaccharide effervescent granules.
In the embodiment, the powdered sugar is prepared by mixing sucrose powder and xylitol according to the mass ratio of 1: 3.
Efficacy experiments:
the active polysaccharide functional drink of example 1 was used as a sample, and the following test for evaluating the cellular immune efficacy of mice was carried out.
The recommended daily dosage of the human body is 200ml/60kg. Experimental animals healthy SPF Kunming mice provided by the Guangdong provincial medical animal center were divided into 2 immunization groups of 40 mice each, and 10 mice each were divided into a water control group and low, medium and high dose groups. Selecting 40 female mice with the weight of 18-22 g for a delayed allergy (DTH) experiment; 40 male mice with the weight of 18-22 g are selected for mouse lymphocyte transformation experiments. The experimental environment temperature is 22 +/-2 ℃, and the relative humidity is 50% +/-10%.
The recommended human dose of the active polysaccharide functional beverage is 200ml per person per day, each person is measured by 60kg body weight, after the active polysaccharide functional beverage is concentrated by 10 times, the active polysaccharide functional beverage is equivalent to 0.33 ml/day/kg.bw, the low, medium and high doses of mice are determined by 5 times, 10 times and 30 times of the recommended human dose, namely the daily eating amount of each group of mice is respectively 1.67ml/kg.bw, 3.33ml/kg.bw and 10ml/kg.bw, the active polysaccharide functional beverage is orally administrated to the mice by intragastric administration once per day, and the intragastric administration volume is 20 ml/kg.bw. Before the gavage, the test substances concentrated by 10 times are prepared by distilled water, the concentrations of the test substances in the low, medium and high dose groups are 83.3ml/L, 166.7ml/L and 500ml/L respectively, the test substances in the control group are replaced by distilled water with the same volume, and the cellular immunity index is tested after the test substances are continuously administered for 30 days. Experimental mice were fed with mouse specific feed.
First, experiment method
1. Delayed type of allergy (thickening of the foot sole) (DTH)
Fresh defibrinated sheep blood was washed 3 times with physiological saline, each rat was intraperitoneally injected with 2% (v/v, formulated with physiological saline) of packed SRBC (2000r/min, l0min)0.2ml, 4 days after sensitization, the thickness of parts of the left hind paw was measured, the thickness of the same part was measured three times, and the average value was taken. The extent of DTH was then expressed as the difference in plantar thickness before and after challenge (degree of swelling of the foot) by subcutaneously injecting 20% (V/V, in saline) of a packed SRBC20 μ l at the measurement site and measuring the thickness of the left hind foot plantar at 24h after injection.
The obtained data is metering data, variance analysis is carried out by SPSS statistical software, and if the difference value of the thickness of the plantar of the foot before and after the attack of the test object group is higher than that of the control group and the difference has significance (P <0.05), the test object can be judged to have the function of improving the delayed allergy ability of the mouse.
ConA-induced splenic lymphocyte transformation assay (MTT method) in mice
The spleen was aseptically removed and placed in a small dish containing sterile Hank's solution to make a cell suspension. The column was filtered through a 200-mesh screen and washed 2 times with Hank's solution and centrifuged l0min at l000r/min each time. Then, the cells were suspended in the complete culture medium of lml RPMI 1640, the number of viable cells was counted, and the cell concentration was adjusted to 3X 106One per ml. The cell suspension was added to a 24-well plate in two wells, each containing 1ml of the cell suspension, and 75. mu.l of ConA solution (equivalent to 7.5. mu.l/ml) was added to one well, and 5% CO was added to the other well as a control237 ℃ CO2After 68h incubation, 0.7ml of supernatant was gently aspirated from each well, and 0.7ml of calf serum-free RPMI 1640 medium was added to each well, together with 5. mu.l of MTT (5mg/ml), and incubation was continued for 4 h. 1ml of acidic isopropanol is added into each well, the mixture is beaten and mixed uniformly to completely dissolve the purple crystals, and then the liquid is transferred into a cuvette and the optical density value (OD value) is measured at a wavelength of 570nm by using an ultraviolet spectrophotometer. And calculating the difference between the OD value of the test hole and the OD value of the control hole, thereby representing the proliferation capacity of the lymphocytes.
The obtained data are measured data, the variance analysis is carried out by SPSS statistical software, and if the proliferation capacity of the lymphocytes of the test object group is higher than that of the control group and the difference is significant (P <0.05), the test object can be judged to have the function of improving the ConA-induced lymphocyte transformation capacity of mice.
And (3) experimental data statistics:
the experimental data is subjected to the homogeneity of variance test on each experimental original data by SPSS software, and data information meeting the 'homogeneity of variance' requirement is subjected to statistical treatment by a pairwise comparison method of mean values between a plurality of experimental groups and a control group; and carrying out proper variable conversion on the data with uneven variance, and carrying out statistical processing on the data obtained by conversion after the requirement of uniform variance is met.
And (3) judging an experimental result:
the result of both experiments in the cellular immune function determination item is positive, or the result of more than one dose group of any experiment is positive, so that the cellular immune function determination result can be judged to be positive.
Second, the experimental result of the effect of the active polysaccharide functional beverage on the immune function of the mouse cells
1. Effect of active polysaccharide functional drink on delayed allergy (DITI) in mice: see table 1.
TABLE 1 Effect of active polysaccharide functional beverages on delayed allergy (DTH) in mice
*P<0.05，**P<0.01
As can be seen from Table 1, after the active polysaccharide functional beverage is orally administered to mice for 30 days at different dosages, the degree of swelling of foot soles of the mice has no significant t growth (P >0.05) when compared between a control group and a low-dose group and has significance when compared between the control group and a medium-dose group and a high-dose group (P <0.05 and P <0.01) after statistical treatment, namely the active polysaccharide functional beverage can enhance the delayed type allergic response capability of the mice.
2. Effect of active polysaccharide functional beverages on ConA-induced mouse lymphocyte transformation experiments: see table 2.
TABLE 2 shadow of ConA-induced mouse lymphocyte transformation experiments with active polysaccharide functional beverages
*P<0.05
As shown in Table 2, after the active polysaccharide functional beverage is orally administered to mice for 30 days at different doses, the lymphocyte proliferation capacity of the mice has no significance (P >0.05) compared with the comparison between the control group and the low dose group and has significance (P <0.05) compared with the comparison between the control group and the medium and high dose groups after statistical treatment, namely the active polysaccharide functional beverage can enhance the lymphocyte transformation capacity of the mice.
The above-mentioned embodiments are only for convenience of description, and are not intended to limit the present invention in any way, and those skilled in the art will understand that the technical features of the present invention can be modified or changed by other equivalent embodiments without departing from the scope of the present invention.
Claims (10)
1. A preparation method of active polysaccharide extract with homology of medicine and food is characterized in that: the method comprises the following steps:
(1) weighing 5-15 parts of astragalus membranaceus, 5-15 parts of rhizoma polygonati, 5-15 parts of poria cocos, 2-10 parts of honeysuckle, 5-15 parts of wolfberry and 0.5-2.5 parts of liquorice, and adding 10 times of water to soak for a period of time;
(2) boiling the soaked liquid medicine at 100 deg.C, adjusting to 80 deg.C, keeping slightly boiling, decocting for the first time, adding 6 times of water into the separated residue, and repeatedly decocting for the second time;
(3) pouring out the liquid medicine decocted twice, and obtaining supernatant after centrifugal separation;
(4) heating the supernatant to 100 ℃ to boil the extracting solution, then cooling to 85 ℃ to keep slight boiling, cooling to room temperature, adding 95% ethanol until the ethanol content is 50%, sealing and standing overnight;
(5) centrifuging the precipitated medicinal liquid again, evaporating the supernatant under reduced pressure, and removing ethanol to obtain the active polysaccharide extractive solution.
2. The preparation method of the active polysaccharide extract as a medicine and food according to claim 1, which is characterized by comprising the following steps: in the step (1), the soaking time is 30-40 min.
3. The preparation method of the active polysaccharide extract as a medicine and food according to claim 1, which is characterized by comprising the following steps: in the step (2), the time for the first decoction is 1h, and the time for the second decoction is 40 min.
4. The preparation method of the active polysaccharide extract as a medicine and food according to claim 1, which is characterized by comprising the following steps: in the step (3), the rotating speed of the centrifugation is 4000r/min, and the centrifugation time is 5 min.
5. The preparation method of the active polysaccharide extract as a medicine and food according to claim 1, which is characterized by comprising the following steps: in the step (5), a rotary evaporator is adopted for reduced pressure evaporation under the conditions of 0.06MPa, 60 ℃ and 1 h.
6. An active polysaccharide functional beverage, which is characterized in that: the active polysaccharide functional beverage is prepared from the following raw materials in parts by weight:
the active polysaccharide extract is prepared by the preparation method of any one of claims 1 to 6.
7. The functional beverage of active polysaccharides according to claim 6, characterized in that: the concentrated fruit juice is concentrated grapefruit juice, concentrated peach and orange juice or concentrated blackcurrant juice.
8. An active polysaccharide effervescent granule, which is characterized in that: the active polysaccharide effervescent granule is prepared from the following raw materials in parts by weight:
the sugar powder is prepared by mixing sucrose powder and xylitol according to the mass ratio of 1: 3;
the active polysaccharide extract is prepared by the preparation method of any one of claims 1 to 6.
9. The process for preparing effervescent granules of active polysaccharide as claimed in claim 8, wherein: the method comprises the following steps:
step a, mixing powdered sugar, citric acid and sodium bicarbonate into auxiliary materials according to a proportion, then slowly spraying the active polysaccharide extracting solution into the mixed auxiliary materials by using a spray bottle on a clean workbench at normal temperature and normal pressure, and stirring while spraying to prepare a soft material;
b, feeding the soft material into a swing type granulator, processing into granules, sieving, drying at 60 ℃, turning over the soft material every 10min in the drying process, and drying to obtain dry granules;
and c, feeding the dry granules into a swing type granulator again for granule stabilization, and sieving to obtain the active polysaccharide effervescent granules.
10. The process for preparing effervescent granules of active polysaccharide according to claim 9, wherein: and the sieving in the step b is 12-mesh sieving, and the sieving in the step c is 14-mesh sieving.
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