CN115125150B - Tricholoma matsutake strain TriMatT35 and application thereof - Google Patents
Tricholoma matsutake strain TriMatT35 and application thereof Download PDFInfo
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- CN115125150B CN115125150B CN202210827762.3A CN202210827762A CN115125150B CN 115125150 B CN115125150 B CN 115125150B CN 202210827762 A CN202210827762 A CN 202210827762A CN 115125150 B CN115125150 B CN 115125150B
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- hair
- tricholoma
- strain
- tricholoma matsutake
- matsutake
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- A—HUMAN NECESSITIES
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- C—CHEMISTRY; METALLURGY
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Abstract
The invention relates to the technical field of microorganisms, in particular to a tricholoma matt strain TriMatt35 and application thereof. The invention provides a tricholoma matsutake (Tricholoma matsutake) strain TriMatT35, which is preserved in China center for type culture collection, and has a preservation number of CCTCC NO: m20211376. The strain has remarkably improved content of nutritional components such as polysaccharide and amino acid, especially cysteine. The strain ferment has hair restoration function, can restore damage of hair outer hair scales, improve hydrophilic lipophilicity and combing property of damaged hair, restore hair internal structure, strengthen hair, and has higher application value in fields of hair cosmetics and the like.
Description
Technical Field
The invention relates to the technical field of microorganisms, in particular to a tricholoma matt strain TriMatt35 and application thereof.
Background
In recent years, more and more people have hair dyeing and hair perming, and the hair dyeing and hair perming can cause damage to hair and scalp, reduce the hair intensity and accelerate the scalp aging. Aiming at the health problems of the hair and the scalp which are concerned, the development of the hair care product with the nourishing and nursing functions for the hair and the scalp is of great significance.
Tricholoma matsutake (Tricholoma matsutake), the subject name Tricholoma matsutake, armillariella matsutake, syzygium, and Theragra, belonging to the family Tricholomataceae, and belonging to the phylum Basidiomycotina, is a mycorrhizal fungus of the exogenesis of trees such as Quercus matsutake. The tricholoma matsutake has unique aromatic flavor, is a wild edible and medicinal fungus rich in various nutrients and functional active ingredients, and has various physiological functions of resisting tumor, enhancing immunity, controlling blood sugar, reducing cholesterol and blood lipid level, and the like. In recent years, due to the destruction and unscientific excavation of forest vegetation, the growth environment of tricholoma matsutake is seriously destroyed, and the requirement of the tricholoma matsutake on the growth environment is relatively high, so that the yield of wild tricholoma matsutake is reduced, and the supply is not required. At present, tricholoma matsutake mainly comes from wild picking, and because the tricholoma matsutake belongs to living symbiotic bacteria, the artificial culture of the tricholoma matsutake is very difficult.
The tricholoma matsutake is rich in various nutritional ingredients, can be used for repairing hair injury, cannot be applied to the field of hair repair on a large scale due to high cost of wild tricholoma matsutake, and at present, although some tricholoma matsutake is artificially cultured, the variety and content of active ingredients of the tricholoma matsutake are greatly reduced compared with those of the wild tricholoma matsutake, so that the application value of the tricholoma matsutake is influenced. Therefore, the development of the tricholoma matsutake strain with higher content of active ingredients and excellent growth performance under the artificial culture condition is of great significance for realizing the application of the tricholoma matsutake in the cosmetic fields such as hair restoration and the like.
Disclosure of Invention
The invention aims at providing a tricholoma matsutake strain TriMatT35. It is another object of the present invention to provide a fermented product of the strain and a product containing the strain or the fermented product thereof. The invention also provides the application of the strain and the fermentation product thereof.
In order to solve the problems existing in the prior art when the tricholoma matsutake strain is artificially cultured, the invention aims to develop the tricholoma matsutake strain with excellent growth performance and higher functional component content during the artificial culture, artificially mutagenize the tricholoma matsutake strain T35 and screen the mutagenized strain with the performance.
Tricholoma matsutake T35 was preserved in China center for type culture Collection (preservation address: university of Wuhan) at 12 and 25 days of 2014, with a preservation number of CCTCC NO: m2014671, which strain is disclosed in Chinese patent CN 105039170A.
Through screening, the invention obtains the tricholoma matsutake strain which has better growth performance during artificial culture and mycelium is rich in functional components such as polysaccharide, amino acid and the like.
Specifically, the invention provides the following technical scheme:
the invention provides a tricholoma matt35 strain which has been preserved in China center for type culture collection (CCTCC for short, address: university of Chinese, wuhan, post code 430072) at the year of 2021, 11 and 5, with a preservation number of CCTCC NO: m20211376, class name Tricholoma matsutake TriMatT.
The invention provides a tricholoma matsutake ferment, which is obtained by fermenting and culturing tricholoma matsutake strain TriMatT35 or extracting the tricholoma matsutake ferment.
The tricholoma matsutake ferment described above may comprise a mixture of one or more of the following (1) - (3):
(1) Mycelium of Tricholoma matt 35;
(2) A metabolite of tricholoma matt 35;
(3) The composition of the fermentation medium.
The mycelium of Tricholoma matt35 strain described above can be either live mycelium or inactivated mycelium.
The metabolites of tricholoma matt35 of the tricholoma matsutake strain described above may include extracellular metabolites and/or intracellular metabolites. Wherein the intracellular metabolite can be obtained by breaking cell wall of mycelium.
As one embodiment of the present invention, there is provided a fermentation product of matt35, which is obtained by fermenting and culturing matt35, and which is a supernatant obtained by subjecting a fermentation broth (which contains cells and a supernatant of the fermentation broth without solid-liquid separation) to sterilization, ultrasonic treatment and enzymolysis treatment, and filtering.
Preferably, the conditions of the sonication are: the ultrasonic frequency is 15-25KHZ, the power is 140-180W, the temperature is 30-37 ℃ and the time is 30-40min.
Preferably, the enzymolysis treatment adopts protease for enzymolysis, and the preferable enzymolysis conditions are as follows: the dosage of bromelain is 1000-3000U/g fermentation liquor, the enzymolysis temperature is 30-50 ℃, and the enzymolysis time is 3-4h.
Specifically, the ferment is prepared by a method comprising the following steps:
(1) Preparing tricholoma matsutake seed liquid: inoculating Tricholoma matsutake strain TriMatT35 into liquid seed culture medium, and culturing at 26deg.C and shaking table of 120-160r/min for 4-6 days;
(2) Inoculating the tricholoma matsutake seed liquid obtained in the step (1) into a fermentation medium according to an inoculum size of 3-8%, and culturing at 26 ℃ and 120-160rpm for 4-6 days to obtain a fermentation liquid;
(3) Sterilizing the fermentation liquor obtained in the step (2), performing ultrasonic treatment on the fermentation liquor, wherein the ultrasonic frequency is 15-25KHZ, the power is 140-180W, the temperature is 30-37 ℃ and the time is 30-40min, then performing enzymolysis on the ultrasonic fermentation liquor by using protease, wherein the dosage of bromelain is 1000-3000U/g fermentation liquor, the enzymolysis temperature is 30-50 ℃ and the enzymolysis time is 3-4h, and filtering and collecting supernatant after enzymolysis to obtain the tricholoma matsutake fermentation product.
The invention also provides a microbial inoculum, which contains the tricholoma matt35 strain or the tricholoma matsutake ferment, or contains the tricholoma matsutake strain TriMatt35 and the ferment thereof.
In the above-described microbial inoculum, the Tricholoma matt35 strain may be present in the form of spores or mycelia.
The formulation of the microbial agent is not particularly limited, and may be a formulation commonly used in the field of microbial agents, for example: the solid microbial inoculum or the liquid microbial inoculum, wherein the solid microbial inoculum comprises freeze-dried spore powder and the like.
Tricholoma matsutake is fungi used as both medicine and food, can be used for preparing various foods and health products, and has medicinal value. The tricholoma matt35 strain provided by the invention grows faster in the artificial culture process, a large amount of mycelia can be harvested in a short time, and meanwhile, the content of functional components such as polysaccharide, amino acid and the like in the mycelia is obviously improved, so that the tricholoma matt35 strain has higher nutritional value and functional activity.
Based on the above functions, the present invention provides any one of the following applications of tricholoma matt35 strain:
the invention provides an application of tricholoma matt35 or tricholoma matsutake ferment or the microbial inoculum in preparing food or medicine.
The invention provides an application of tricholoma matt35 or tricholoma matsutake ferment or the microbial inoculum in preparing health care products.
The food, medicine and health product can be preferably products with the functions of resisting oxidation, enhancing immunity, resisting tumor, preventing and treating diabetes, cardiovascular diseases and the like.
The invention also provides an application of the tricholoma matt35 strain or the tricholoma matsutake ferment or the microbial inoculum in preparing cosmetics.
The above-mentioned cosmetics include skin cosmetics, hair cosmetics, beauty cosmetics and the like, wherein the hair cosmetics are cosmetics for cleaning, protecting and nourishing the hair of beautifiers, including shampoos (shampoos ), conditioners, hair sprays, temporary spray hair color (non-hair coloring) shampoos, hair styling products, hair oils, hair waxes, hair creams and the like.
The tricholoma matt35 strain has good hair or scalp care and repair functions, and can effectively nourish and repair damaged hair and scalp caused by perming, dyeing, environmental factors and the like.
Based on the above, the invention provides an application of tricholoma matt35 strain or tricholoma matt ferment or the microbial inoculum in preparing products with hair or scalp care and repair functions.
The invention provides a food which contains the tricholoma matt strain TriMatt35 or the tricholoma matsutake ferment.
The food product described above may also contain other materials (e.g., sweeteners, acidulants, etc.) as permitted in the food arts.
The invention provides a cosmetic, which contains the tricholoma matsutake ferment.
Preferably, the cosmetic is a hair cosmetic. More preferably, the cosmetic is a shampoo-type cosmetic.
The shampoo cosmetic comprises tricholoma matsutake fermentation product and a matrix of the shampoo cosmetic allowed in the field of shampoo cosmetics.
Preferably, the mass percentage of the tricholoma matsutake ferment in the cosmetic is 0.1-15%.
The preparation method of the tricholoma matsutake ferment comprises the following steps: fermenting and culturing the tricholoma matt35 strain to obtain fermentation liquor, sterilizing, performing ultrasonic and enzymolysis treatment on the fermentation liquor, filtering, and collecting supernatant.
Preferably, the enzymolysis is carried out by using protease, wherein the protease is bromelain, the consumption of the protease is 1000-3000U/g fermentation liquor, and the enzymolysis conditions are as follows: the enzymolysis temperature is 30-50 ℃ and the enzymolysis time is 3-4h.
The conditions of the above-described ultrasonic treatment are preferably: the ultrasonic frequency is 15-25KHZ, the power is 140-180W, the temperature is 30-37 ℃ and the time is 30-40min.
The sterilization conditions described above are preferably: sterilizing at 115-121deg.C for 15-25min.
The medium used in the above-described fermentation culture includes a carbon source and a nitrogen source. Wherein the carbon source comprises a sugar and the nitrogen source comprises an organic nitrogen source.
Specifically, the carbon source is preferably one or more selected from glucose, ribose, lactose, galactose, fructose, sucrose, maltose, mannose, trehalose, mannitol, sorbitol. The organic nitrogen source is preferably one or more selected from peptone, beef extract powder, yeast extract powder.
A more suitable carbon and nitrogen source combination mode of the tricholoma matt35 strain is as follows: the carbon source is glucose, and the nitrogen source is peptone, beef extract powder and yeast extract powder.
Preferred fermentation media comprise the following components (g: ml): glucose 0.5-3%, peptone 0.5-1.5%, beef extract powder 0.5-1.5%, yeast extract powder 0.2-0.8%, dipotassium hydrogen phosphate 0.01-0.05%, magnesium sulfate 0.01-0.05%, V B1 0.005-0.02％。
The fermentation culture conditions are preferably as follows: culturing at 26 deg.C and 120-160rpm for 4-6 days.
The method also comprises the step of preparing seed liquid before fermentation culture, wherein the seed culture medium preferably comprises the following components (g: ml): glucose 0.5-3%, peptone 0.5-1.5%, yeast extract powder 0.2-0.8%, dipotassium hydrogen phosphate 0.01-0.05%, and magnesium sulfate 0.01-0.05%.
The seed culture conditions are preferably: culturing at 26 deg.C for 4-6 days at 120-160 r/min.
The tricholoma matt35 strain is fermented and cultured to obtain mycelium with higher content of functional components, so that the utilization efficiency of the functional components of the tricholoma matsutake is improved. The tricholoma matsutake ferment obtained by the preparation method can effectively repair damaged hair, and has higher application value in hair washing and caring products.
The invention has the beneficial effects that: the invention provides a tricholoma matt35 strain which has higher genetic stability and does not degenerate after more than 10 times of subculture; the strain has better growth performance in artificial culture, can obtain higher mycelium yield in a shorter time, improves the production efficiency of the tricholoma matsutake mycelium and reduces the production cost. In addition, the mycelium of the tricholoma matt35 strain contains high content of polysaccharide and amino acid, has high nutritional value and efficacy, particularly has good hair restoration function, can restore damage to hair outer hair scales, improve hydrophilic lipophilicity and carding property of damaged hair, restore internal structure of hair, strengthen hair, and has high application value in fields of hair cosmetics and the like.
Drawings
FIG. 1 is a result of a growth performance test of Tricholoma matt35 strain in example 2 of the present invention.
FIG. 2 shows the results of examining the comb property of a shampoo for repairing wet hair in the experimental example of the present invention.
FIG. 3 shows the results of the test of the comb property of the shampoo for repairing dry hair in the experimental example of the invention.
Detailed Description
The following examples are illustrative of the invention and are not intended to limit the scope of the invention.
Example 1 obtaining Tricholoma matt35 Strain
The preservation number of the tricholoma matsutake strain is CCTCC NO: the tricholoma matsutake strain T35 of M2014671 is taken as an original strain, and ultraviolet mutagenesis treatment and screening of mutagenized strains are carried out on the strain, and the specific method is as follows:
(1) The preservation number is CCTCC NO: activating Tricholoma matsutake strain T35 of M2014671, inoculating to PDA slant, culturing at 26deg.C for 5 days, adding phosphate buffer solution with pH of 6.0 into slant culture medium, filtering with 4 layers of gauze, and diluting spore concentration to 10 6 -10 7 The number of the seeds per mL is used for mutation breeding;
(2) Irradiating the spore suspension obtained in the step (1) with 15w of ultraviolet rays for 2min, diluting, coating a flat plate, placing in a constant temperature box at 26 ℃ for 2 days, and performing point-to-point implantation of sterilized toothpicks into a 96-well plate filled with PDA solid culture medium for culture;
inoculating the cultured mutant strains into a 250mL conical flask one by using an inoculating needle for culturing at 26 ℃ for 5 days at 150rpm to obtain tricholoma matsutake mycelia, respectively measuring the growth conditions of the mycelia and the contents of substances such as mycelium polysaccharide, amino acid and the like, and screening tricholoma matsutake strains with good growth performance and high content of effective components;
(3) And (3) continuously carrying out passage on the obtained tricholoma matsutake mutant strain, culturing the tricholoma matsutake mutant strain in a shaking table at a constant temperature of 150rpm at 26 ℃ for 90-120 hours, measuring mycelium polysaccharide and amino acid, and examining the genetic stability of the mutant strain.
Finally obtaining the tricholoma matt35 strain with better growth performance and higher polysaccharide and amino acid content in mycelium through ultraviolet mutagenesis and screening.
Tricholoma matt35 strain has been preserved in China center for type culture collection (CCTCC, address: university of Wuhan, post code 430072) at 2021, 11 months and 5 days, with a preservation number of CCTCC NO: m20211376, class name Tricholoma matsutake TriMatT.
Example 2 Performance test of Tricholoma matt35 Strain
1. Growth performance test
Inoculating Tricholoma matsutake strain TriMatT35 and Tricholoma matsutake strain T35 into liquid seed culture medium, respectively culturing at 26deg.C and 150r/min shaking table for 1,3,5d, measuring mycelium yield, and drawing growth curves of Tricholoma matsutake strains TriMatT35 and T35, wherein the seed culture medium comprises the following components: glucose 2%, peptone 1%, yeast extract 1%, dipotassium hydrogen phosphate 0.02%, magnesium sulfate 0.03%, V B1 0.01％。
As a result, as shown in FIG. 1, it was revealed from the graph that the Tricholoma matsutake strain TriMatT35 had a significantly higher growth rate than T35, and that the mycelium yield was significantly higher than T35.
2. Functional ingredient content detection
Mixing Tricholoma matsutake strain TriMatT35 Tricholoma matsutake T35 and other Tricholoma matsutake strains (named Tricholoma matsutake strains 1, 2, 3, 4) obtained in the mutagenesis screening process of example 1 are inoculated into liquid seed culture medium (glucose 2%, peptone 1%, yeast extract 1%, dipotassium hydrogen phosphate 0.02%, magnesium sulfate 0.03%, V) B1 0.01%), culturing at 26deg.C with 150r/min shaking table for 5d, centrifuging mycelium at 5000rpm, washing with water for 2 times, lyophilizing mycelium, pulverizing, and sieving with 80 mesh sieve. Three parts of dry powder of mycelia of Tricholoma matsutake 0.1g are weighed respectively, 5mL of distilled water is added respectively, water bath extraction is carried out at 80 ℃ for 3h,5000g/min is centrifuged for 6min, and the supernatant is taken to determine the contents of polysaccharide, total amino acids and cysteine of mycelia, and the results are shown in Table 1.
TABLE 1 content of functional ingredients
| Polysaccharide g/g | Amino acid mg/g | Cysteine mg/g | |
| Original strain (T35) | 0.117 | 82 | 12 |
| Tricholoma matsutake strain TriMatT35 | 0.131 | 116 | 33 |
| Tricholoma matsutake Strain 1 | 0.123 | 102 | 23 |
| Tricholoma matsutake Strain 2 | 0.119 | 98 | 21 |
| Tricholoma matsutake Strain 3 | 0.116 | 90 | 17 |
| Tricholoma matsutake Strain 4 | 0.118 | 89 | 16 |
3. Genetic stability detection
Inoculating Tricholoma matt35 strain into liquid seed culture medium (glucose 2%, peptone 1%, yeast extract 1%, dipotassium hydrogen phosphate 0.02%, magnesium sulfate 0.03%, V) B1 0.01%), subculturing, and measuring polysaccharide and amino acid content of mycelium of the strain every other generation, and culturing for 10 generations, and examining genetic stability of the strain, and the results are shown in Table 2.
Table 2 stability investigation of mutagenized strains
Example 3 fermentation culture of Tricholoma matt35 Strain and preparation of ferment
Fermenting and culturing Tricholoma matt35 strain to prepare Tricholoma matsutake ferment, and specifically comprises the following steps:
(1) Preparation of matsutake seed liquid
Inoculating Tricholoma matsutake strain TriMatT35 into liquid seed culture medium, and culturing at 26deg.C and 150r/min shaking table for 5d to obtain seed solution; the seed medium consisted of: glucose 2%, peptone 1%, yeast extract 1%, dipotassium hydrogen phosphate 0.02%, magnesium sulfate 0.03%, V B1 0.01％；
(2) Fermentation culture
Inoculating the tricholoma matsutake seed liquid prepared in the step (1) into a fermentation medium according to an inoculum size of 5%, and culturing at 26 ℃ and 150rpm for 5 days to obtain a fermentation liquid; the fermentation medium consisted of: glucose 2%, peptone 1%, yeast extract 1%, dipotassium phosphate 0.02% and magnesium sulfate 0.03%;
(3) Preparation of tricholoma matsutake ferment
Sterilizing the fermentation liquor obtained in the step (2), sterilizing at 121 ℃ for 20min, then carrying out ultrasonic treatment on the sterilized fermentation liquor, wherein the ultrasonic treatment frequency is 20KHZ, the power is 160W, the temperature is 35 ℃, the time is 35min, then carrying out enzymolysis on the ultrasonic-treated fermentation liquor by using bromelain, the protease dosage is 2600U/g of fermentation liquor, the enzymolysis temperature is 39 ℃, the enzymolysis time is 3.6h, filtering, collecting supernatant, and cooling to room temperature to obtain the tricholoma matsutake fermentation product.
Example 4 shampoo containing Tricholoma matsutake Strain TriMatT35 fermentation
The embodiment provides shampoo, which comprises the following components in percentage by mass: 1% of tricholoma matsutake ferment of example 3 and 99% of shampoo matrix.
Wherein, the shampoo matrix comprises the following components (mass percent): 15% of laureth ammonium sulfate, 3% of methyl myristoyl taurate, 7% of lauramidopropyl betaine, 0.5% of cocamide MEA, 0.1% of polyquaternium-47%, 0.2% of cocoyl hydrolyzed soy protein, 0.2% of sodium diiodoamidopropyl PG-dimethyl ammonium chloride phosphate, 0.2% of guar hydroxypropyl trimethyl ammonium chloride, 0.1% of essence, 0.1% of iodopropynyl butyl carbamate and 71.8% of deionized water.
Comparative example 1 shampoo containing Tricholoma matsutake Strain T35 fermentation product
The comparative example provides a shampoo which comprises the following components in percentage by mass: 1% of a fermentation product of T35 tricholoma matsutake and 99% of a shampoo matrix, wherein the preparation method of the fermentation product of T35 tricholoma matsutake is the same as that of the fermentation product of tricholoma matsutake strain TriMatT35 in example 3, except that the tricholoma matsutake strain TriMatT35 is replaced by the tricholoma matsutake strain T35.
Wherein the composition of the shampoo base was the same as that of the shampoo base in example 4.
Comparative example 2 shampoo base
This comparative example provides a shampoo base having the same formulation as the shampoo base of example 4.
Experimental example Hair restoration function detection of Tricholoma matt35 fermentation product
The shampoo of example 4 and comparative example 1 and the shampoo base of comparative example 2 were subjected to hair restoration function test, and the test method and test result were specifically as follows:
1. experiment of damaged effect of shampoo on repairing damaged hair and external hair scales
Healthy hair of volunteers is selected, washed by a sodium dodecyl sulfate solution, rinsed and naturally dried for later use. Taking 4 parts of intact hair, 10g each, soaking with 0.5g/L NaOH at 30deg.C for 1 hr, taking out, rinsing to neutrality, and naturally air drying to obtain damaged hair.
The shampoo of example 4 and comparative example 1 and the shampoo of comparative example 2 were used to wash damaged hair, the shampoo amount was 0.5g, the washing time was 3min, and the hair was rinsed with clear water and naturally dried.
The untreated (blank) and shampoo-washed hair bundles were immersed in a 0.2mol/L copper sulfate solution for 15min, respectively, and then the amount of copper ions adsorbed by the hair was measured.
TABLE 3 copper ion adsorption quantity
Note that: * P <0.05, P <0.01, P <0.001vs. blank.
When hair is damaged, the hair is negatively charged to absorb copper ions, and the hair damage degree can be characterized by measuring the copper ion absorption amount of the hair. The greater the degree of hair damage, the more copper ions are adsorbed. As can be seen from table 3, the copper ion adsorption amount of hair treated with shampoo was significantly reduced compared with the blank, while the copper ion adsorption amount of hair treated with shampoo containing tricholoma matt35 fermentation was significantly reduced compared with the shampoo containing tricholoma matt35 fermentation, indicating that the tricholoma matt35 fermentation has a more significant repair effect on hair external hair scale damage.
2. Hydrophilic-lipophilic test for repairing damaged hair by shampoo
30 volunteers subjected to dyeing and scalding within three months are selected, the hair contact angle (dry hair is 68-72 degrees, wet hair is 66-70 degrees) of each volunteer is tested, the volunteers are randomly divided into 3 groups, 10 people in each group are collected, the hair of each volunteer is recorded as a blank group, the damaged hair is respectively cleaned by using the shampoo of the example 4 and the shampoo of the comparative example 1 and the shampoo of the comparative example 2, 3 times per week, the hair of each volunteer is collected once per week, after the 3-week experiment is finished, the hair of each group is inserted into water in two states of dry hair and wet hair by adopting a Wilhelmy balance method, and the contact angle of water on the hair surface is calculated according to the received force, and the result is shown in Table 4.
TABLE 4 Hair contact angle
Note that: * P <0.05, P <0.01, P <0.001vs. blank.
The degree of hair damage is directly related to the hydrophilic and hydrophobic properties of the hair surface, and the greater the degree of hair damage, the greater the hydrophilicity of the hair. After the liquid spreads on the solid surface, the solid/liquid interface passes through the liquid interior to the gas/liquid interface, which is called the contact angle. The smaller the contact angle of water on the hair surface, the easier the spreading and the more hydrophilic. As shown in table 4, the contact angle of hair was remarkably increased by using the shampoo of example 4, compared with the hair dyed and scalded (blank group), and it was statistically significant (P < 0.05), indicating that the tricholoma matt35 fermented product of tricholoma matsutake strain was capable of remarkably repairing hair damage and restoring the hydrophilic and lipophilic properties of hair.
3. Influence of shampoo restoration on hair comb properties
The effect of shampoo restoration of example 4 on hair combability was analyzed as follows:
30 volunteers subjected to dyeing and scalding within three months are selected, randomly divided into 3 groups, 10 volunteers are collected for hair leave-out before the test starts, each group of volunteers is respectively washed by using shampoo of example 4 and comparative example 1 and shampoo matrix of comparative example 2, used for 1 week and used once every other day, and hair of each volunteer is collected after the test is finished.
The collected hair is washed by tap water, the towel is used for wiping off redundant water until the hair bundle is free from dripping, the hair bundle is combed smoothly by a comb, and the wet hair combability is tested by a tension tester. The test results are shown in fig. 2.
Then, the hair is blown to be semi-dry by a blower at a distance of 20cm from the hair bundle, the hair is combed smoothly by a comb, the hair is naturally dried, and the combability of the dry hair is tested by a universal tensile tester. The test results are shown in FIG. 3.
The results show that the shampoo of example 4 has significantly better combing properties (wet combing and dry combing) on hair than the shampoo containing the fermentation broth of Tricholoma matsutake strain T35 (comparative example 1) and the shampoo base (comparative example 2).
4. Shampoo repair dyeing and scalding damaged hair effect experiment
To verify the effect of the shampoo of example 4 on repairing and scalding damaged hair, 30 volunteers who had been dyed and scalded in the age of 25-60 years and had suffered a certain damage to hair during the dyeing and scalding process were selected, and were randomly divided into 3 groups of 10 persons each, and the shampoo of example 4, the shampoo of comparative example 1 and the shampoo base of comparative example 2 were provided for 2 months at a frequency of once every two days, and the effects of the shampoo on repairing the internal structure of hair and strengthening hair were examined. Volunteers measured maximum force and work of breakage of hair before the start of the experiment, measured maximum force and work of breakage of hair after 2 months using shampoo, and compared to changes in hair strength. Hair strength was tested using a Diastron tensiometer.
TABLE 5 variation of hair breaking strength
The results are shown in Table 5, and the maximum force of hair breakage and breaking work are significantly increased after the shampoo of example 4 compared with the shampoo before the use, which indicates that the fermentation product of Tricholoma matt35 strain can significantly repair the internal structure of hair and improve the hair strength.
While the invention has been described in detail in the foregoing general description and with reference to specific embodiments thereof, it will be apparent to one skilled in the art that modifications and improvements can be made thereto. Accordingly, such modifications or improvements may be made without departing from the spirit of the invention and are intended to be within the scope of the invention as claimed.
Claims (8)
1. Tricholoma matsutake (Tricholoma matsutake) strain TriMatT35 is characterized in that the Tricholoma matsutake is preserved in China center for type culture collection, and the preservation number is CCTCC NO: m20211376.
2. The tricholoma matsutake mycelium is obtained by fermenting and culturing the tricholoma matsutake (Tricholoma matsutake) strain TriMatT35 according to claim 1 or by fermenting and culturing and extracting.
3. The microbial inoculum, characterized in that it contains the Tricholoma matt35 strain (Tricholoma matsutake) as claimed in claim 1 and/or the Tricholoma matsutake mycelium as claimed in claim 2.
4. Use of the tricholoma matt35 strain of tricholoma matsutake (Tricholoma matsutake) as claimed in claim 1 or the tricholoma matsutake mycelium as claimed in claim 2 or the microbial inoculum as claimed in claim 3 in the preparation of a food or medicament.
5. Use of the tricholoma matt35 strain of tricholoma matsutake (Tricholoma matsutake) as claimed in claim 1 or the tricholoma matsutake mycelium as claimed in claim 2 or the microbial inoculum as claimed in claim 3 in the preparation of health care products.
6. Use of the tricholoma matt35 strain of tricholoma matsutake (Tricholoma matsutake) as claimed in claim 1 or the tricholoma matsutake mycelium as claimed in claim 2 or the microbial inoculum as claimed in claim 3 in the preparation of cosmetics.
7. Use of the tricholoma matt35 strain of tricholoma matsutake (Tricholoma matsutake) as claimed in claim 1 or the tricholoma matsutake mycelium as claimed in claim 2 or the microbial inoculum as claimed in claim 3 in the preparation of products with hair or scalp care and restoration functions.
8. A food product comprising the tricholoma matt35 strain of tricholoma matsutake (Tricholoma matsutake) of claim 1 or the tricholoma matsutake mycelium of claim 2.
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Citations (5)
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| WO2002030440A1 (en) * | 2000-10-11 | 2002-04-18 | Kureha Chemical Industry Co., Ltd. | Medicinal compositions for promoting recovery from stress loading and novel matsutake mushroom strain |
| CN104906149A (en) * | 2015-05-28 | 2015-09-16 | 吉林大学 | Medical application of tricholoma matsutake high-yield strain (T35) in resisting fatigue |
| CN105039170A (en) * | 2015-05-28 | 2015-11-11 | 吉林大学 | High-yield Tricholoma matsutake sing mutagenesis strain and breeding method thereof |
| CN106376914A (en) * | 2016-08-25 | 2017-02-08 | 山东天博食品配料有限公司 | Method for preparation of tricholoma matsutake refined powder from tricholoma matsutake submerged fermentation mycelium |
| CN114667890A (en) * | 2021-12-20 | 2022-06-28 | 延边圣泽方圆生物科技有限公司 | Method for separating and culturing strain and mycelium from Tricholoma matsutake fruiting body, and preparation method of Tricholoma matsutake mycelium powder and concentrated solution |
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| WO2002030440A1 (en) * | 2000-10-11 | 2002-04-18 | Kureha Chemical Industry Co., Ltd. | Medicinal compositions for promoting recovery from stress loading and novel matsutake mushroom strain |
| CN104906149A (en) * | 2015-05-28 | 2015-09-16 | 吉林大学 | Medical application of tricholoma matsutake high-yield strain (T35) in resisting fatigue |
| CN105039170A (en) * | 2015-05-28 | 2015-11-11 | 吉林大学 | High-yield Tricholoma matsutake sing mutagenesis strain and breeding method thereof |
| CN106376914A (en) * | 2016-08-25 | 2017-02-08 | 山东天博食品配料有限公司 | Method for preparation of tricholoma matsutake refined powder from tricholoma matsutake submerged fermentation mycelium |
| CN114667890A (en) * | 2021-12-20 | 2022-06-28 | 延边圣泽方圆生物科技有限公司 | Method for separating and culturing strain and mycelium from Tricholoma matsutake fruiting body, and preparation method of Tricholoma matsutake mycelium powder and concentrated solution |
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