CN110559241A

CN110559241A - Composition with moisturizing and relieving effects and preparation method and application thereof

Info

Publication number: CN110559241A
Application number: CN201910796090.2A
Authority: CN
Inventors: 秦允荣; 徐勇威; 张达成
Original assignee: Guangzhou Hong Zhong Biotechnology Co Ltd
Current assignee: Guangzhou Hong Zhong Biotechnology Co Ltd
Priority date: 2019-08-27
Filing date: 2019-08-27
Publication date: 2019-12-13

Abstract

The invention discloses a composition with moisturizing and relieving effects, a preparation method and an application thereof, wherein the composition comprises the following components in parts by weight: 20-50 parts of oat extract, 20-50 parts of tremella extract, 10-30 parts of cactus extract and 5-20 parts of seaweed extract, and comprises the steps of material preparation, fermentation extraction and freeze-drying, and the composition with the moisturizing and relieving effects is high in stability and long in shelf life, and can keep the activity of active ingredients to the maximum extent; the composition can be applied to products to improve the skin moisturizing capability, promote the self-hydration, water storage and water distribution of the skin, reduce the water loss of the skin, reduce the skin sensitivity and repair damage and restore the skin to a healthy state.

Description

Composition with moisturizing and relieving effects and preparation method and application thereof

Technical Field

The invention relates to a composition, in particular to a composition with moisturizing and relieving effects, a preparation method and application thereof.

background

The primary conditions for a healthy, moist, elastic, glossy skin are sufficient moisture in the skin and good moisture retention of the skin. Most of the existing cosmetic means can improve the moisture content of skin by external moisture-retaining components, so that the skin texture can be immediately improved, the subsequent skin-care effect is promoted, and the makeup is smooth and comfortable, so that the cosmetic with water replenishing and moisture retaining functions is a basic skin-care product which is most demanded by consumers.

most moisturizing products in the market are added with a large amount of moisturizing agents to form a film for preventing moisture evaporation on the surface layer of skin, and although the moisturizing products have a certain moisturizing effect, the problem of skin dryness cannot be fundamentally solved, and the moisturizing products also have the defects of greasy skin feel and short duration. As is well known, many natural active ingredients contain polysaccharide, amino acid, vitamin, polyphenol and other ingredients, so that the skin can be well moisturized, relieved, aged and conditioned, and the skin can be restored to a healthy state of water-wet elasticity, but the effect of the raw materials is difficult to be expected due to the limitations of extraction solvents, processes, preparation methods and the like of extracts prepared by the traditional process, and although the fermentation technology can extract smaller-molecular active nutrient substances from natural plant resources, the effect is enhanced, the toxicity is reduced, the effectiveness and the absorbability of the raw materials are improved, the activity of the raw materials is ensured by additionally adding preservative ingredients, and the introduction of the preservative ingredients has the hidden danger of sensitization.

Disclosure of Invention

in order to overcome the defects and shortcomings in the prior art, the invention aims to provide a natural and safe composition with moisturizing and relieving effects.

In order to achieve the purpose of the invention, the invention adopts the following technical scheme:

A composition with moisturizing and relieving effects comprises the following components in parts by weight: 20-50 parts of oat extract, 20-50 parts of tremella extract, 10-30 parts of cactus extract and 5-20 parts of seaweed extract.

further preferably, the composition with moisturizing and soothing effects comprises the following components in parts by weight: 30-50 parts of oat extract, 20-40 parts of tremella extract, 10-30 parts of cactus extract and 5-20 parts of seaweed extract.

further preferably, the composition with moisturizing and soothing effects comprises the following components in parts by weight: 40-50 parts of oat extract, 20-40 parts of tremella extract, 10-20 parts of cactus extract and 10-20 parts of seaweed extract.

Further preferably, the composition with moisturizing and soothing effects comprises the following components in parts by weight: 30 parts of oat extract, 40 parts of tremella extract, 20 parts of cactus extract and 10 parts of seaweed extract.

The second purpose of the invention is to provide a preparation method of the composition with moisturizing and relieving effects.

A method for preparing a composition with moisturizing and soothing effects comprises the following steps:

(1) preparing materials: respectively preparing filtrate of oat, tremella, cactus and seaweed for later use;

(2) fermentation and extraction: fermenting the filtrate obtained in the step (1) to respectively prepare oat, tremella, cactus and seaweed extracts, and specifically comprises the following steps:

And (3) oat extract: according to the method, 5-50 mL of zymophyte liquid I, honey and oat filtrate: 5-30 g: selecting raw materials according to the proportion of 100-300 g, uniformly mixing, fermenting the prepared solution in the environment of 25-32 ℃ for 7-14 days, centrifuging for 15-30min at 8000-10000r/min, and filtering to obtain supernatant, namely the oat extract;

White fungus extract: according to the following steps, 5-20 mL of zymophyte liquid II, honey and tremella filtrate: 10-30 g: selecting raw materials according to the proportion of 50-100 g, uniformly mixing, fermenting the prepared solution at the temperature of 35-42 ℃ for 5-7 days, centrifuging at 8000-10000r/min for 15-30min, and filtering to obtain a supernatant, namely the tremella extract;

Cactus extract: according to the following steps, 1-10 mL of zymogen liquid III, honey and cactus filtrate: 5-10 g: selecting raw materials according to the proportion of 50-200 g, uniformly mixing, fermenting the prepared solution in the environment of 25-30 ℃ for 7-10 days, centrifuging for 15-30min at 8000-10000r/min, and filtering to obtain supernatant, namely the cactus extract;

and (3) seaweed extract: and (3) setting the fermentation bacterium liquid IV, the honey and the seaweed filtrate to be 5-20 mL: 20-50 g: selecting raw materials according to the proportion of 100-300 g, uniformly mixing, fermenting the prepared solution in the environment of 25-30 ℃ for 5-10 days, centrifuging for 15-30min at 8000-10000r/min, and filtering to obtain supernatant, namely the seaweed extract;

(3) freeze-drying: according to the proportion of the oat extract, the tremella extract, the cactus extract and the seaweed extract in the step (2) being 20-50: mixing and stirring uniformly according to the ratio of 10-30: 5-20, filling the extract mixture into a freezing bottle, and taking out a product after freeze drying to obtain the composition with the effects of moisturizing and relieving.

Preferably, the specific preparation steps of the filtrate in the step (1) are as follows:

Oat filtrate: sieving the oat subjected to impurity removal and crushing by using a sieve of 80-200 meshes to obtain oat powder, and sieving the oat powder according to the weight ratio of amylase: oat flour: deionized water 0.001 g: 10-30 g: preparing 100-300 ml of solution, carrying out enzymolysis on the solution at the temperature of 32-38 ℃ and the pH value of 5.8-6.2 for 40-60 min, and filtering to obtain filtrate for later use;

White fungus filtrate: sieving the tremella subjected to impurity removal and crushing by a sieve of 80 meshes, and mixing the tremella powder: taking materials from 1: 20-1: 40 of deionized water, adding tremella powder into the deionized water, mixing and pulping, collecting slurry, adding 0.03-0.06% of pectinase into the slurry, performing enzymolysis for 2-4 h at 35-38 ℃, and sieving with a 200-mesh sieve to obtain filtrate for later use;

Cactus filtrate: cleaning cactus, removing thorns and peels, taking mesophyll, chopping, homogenizing for 1-3 min by a homogenizer, and filtering to obtain filtrate for later use;

Seaweed filtrate: cutting the dried seaweed after rehydration into blocks, adding deionized water according to the mass ratio of the material liquid of 1: 5-1: 10, mixing and pulping, collecting pulp, adding 0.03-0.06% of food grade pectase, performing enzymolysis for 1-3 h at 35-38 ℃, filtering with 200-mesh gauze, and taking filtrate for later use.

preferably, in the step (2), the zymogen liquid I is Saccharomyces Cerevisiae (Saccharomyces Cerevisiae) in the form of suspension, and the concentration of the zymogen liquid I is 10⁶～10⁸cfu/ml, pH of6.5-7.5; the zymocyte liquid II is prepared by mixing Lactobacillus plantarum (Lactobacillus plantarum) and Saccharomyces Cerevisiae (Saccharomyces Cerevisiae) according to the weight ratio of 2: 1, at a concentration of 10⁶～10⁸cfu/ml, pH value is 6.5-7.5;

The zymocyte liquid III is prepared by mixing Lactobacillus delbrueckii, Lactobacillus fermentum and Saccharomyces Cerevisiae in a ratio of 2: 3: 1, in a concentration of 10⁶～10⁸cfu/ml, pH value is 6.5-7.5;

The fermentation bacterium liquid IV is prepared by mixing Lactobacillus plantarum (Lactobacillus plantarum), Lactobacillus fermentum (Lactobacillus fermentum) and Lactobacillus acidophilus (Lactobacillus acidophilus) in a ratio of 1: 1:1, in a concentration of 10⁶～10⁸cfu/ml, pH 6.5-7.5.

Preferably, in the step (3), the freezing comprises the following specific steps: pre-freezing the mixture extract at 0-4 ℃ for 1-3 h, freezing at-5-10 ℃ for 2-4 h, finally freezing at-40 ℃ for 4-6 h, taking out, rapidly putting into a precooled freeze dryer, and carrying out sublimation drying for 12-18 h under the pressure of 20-30 PaPa. And stopping the machine and emptying the machine after the materials are completely dried, and taking out the product to obtain the composition with the moisturizing and relieving effects.

The third purpose of the invention is to provide the application of the composition with moisturizing and relieving effects in cosmetics.

Preferably, the cosmetic comprises at least one of a lotion, a milk, a serum, a cream, a mask, a face cleanser, or a BB cream.

Preferably, the cosmetic contains a composition with moisturizing and relieving effects, wherein the addition amount of the composition is 0.05-1%.

Further preferably, the cosmetic contains a composition with moisturizing and relieving effects, wherein the addition amount of the composition is 0.1-0.5%.

Specifically, the invention provides moisturizing essence with a moisturizing effect, which comprises the following components in parts by mass:

in the invention, in the process of long-term production research, the inventor deeply excavates effective species reasonable compounding with water replenishing, moisture preserving, skin moistening, skin nourishing, soothing and sensitivity in raw materials: oat has natural health care function and high beauty value, people have long understood that oat is used for treating dry skin and itching, contains protein, vitamin, beta-glucan, oat oil and other components, and has the effects of resisting oxidation, increasing skin activity, delaying skin aging, whitening and moisturizing, reducing wrinkles and stains, resisting allergy and the like; tremella contains abundant mucopolysaccharides such as glucose, trehalose, pentose polyacid, mannitol and the like, has a structure very similar to that of hyaluronic acid, has excellent moisturizing capability, smaller molecules and higher absorption and permeation speed, and is a natural high-quality moisturizing component; the fresh cactus contains rich protein, mineral substances, cellulose, vitamin C and the like, and also contains rich active functional components such as SOD, flavonoids, polysaccharides and the like, so that the cactus can moisturize, store water, protect skin, resist inflammation and infection and promote the regeneration and repair of cells; the seaweed contains rich sulfated polysaccharide and viscous polysaccharide, is an excellent emollient, and active substances in the seaweed can be combined with skin protein to form a moisturizing gel, and can form a protective film on the surface of the skin through hydration to prevent water evaporation, and meanwhile, the seaweed also has the effects of resisting bacteria, diminishing inflammation, controlling oil, removing acnes and repairing damage.

Compared with the prior art, the invention has the following beneficial effects:

According to the invention, the four composition extracts are applied to cosmetics in a freeze-drying manner after fermentation treatment, the composition can be transported and stored at normal temperature, has long shelf life, and can furthest maintain the activity and value of active ingredients; the composition of the invention comprises a plurality of components with moisturizing and relieving effects, the effects of the components are coordinated, the water locking and water producing capability of the skin can be enhanced, the skin can be helped to recover the moisturizing elasticity, the skin defense capability can be enhanced, and the skin sensitivity and repair damage can be resisted.

Detailed Description

The present invention will be described in further detail with reference to examples, but the embodiments of the present invention are not limited thereto.

Example 1 composition (I) having moisturizing and soothing effects and preparation method thereof

a composition with moisturizing and relieving effects is prepared from the following components in parts by weight in a formula table:

Components	Parts by weight
		Oat extract	40
Tremella extract	30
		Opuntia ficus extract	23
Seaweed extract	7

The preparation method comprises the following steps:

1) Pretreatment of

Crushing the naked oat which is sorted out and has been subjected to impurity and particle screening, sieving the crushed naked oat by a 200-mesh sieve, and mixing the crushed naked oat with the amylase, the oat powder and the deionized water according to the dosage ratio of 0.001 g: 15 g: 200ml, enzymolyzing at 35 deg.c and pH6.0 for 50min, filtering and taking the filtrate for further use.

Crushing tremella, sieving with a 80-mesh sieve, adding into deionized water according to the feed liquid mass ratio of 1:25, mixing and pulping, collecting pulp, adding 0.05% of pectinase into the pulp, performing enzymolysis for 2h at 37 ℃, sieving with a 200-mesh sieve, and taking the filtrate for later use.

cleaning radix et caulis Opuntiae Dillenii, removing spines and skin, cutting mesophyll, homogenizing for 2min with homogenizer, and filtering to obtain filtrate.

Cutting the dried seaweed after rehydration into blocks, adding deionized water according to the mass ratio of the material liquid of 1: 5-1: 10, mixing and pulping, collecting pulp, adding 0.05% of food grade pectase, performing enzymolysis for 1-3 h at 37 ℃, filtering with 200-mesh gauze, and taking filtrate for later use. Wherein the seaweed consists of kelp and laver in a ratio of 1: 2.

2) Fermentation extraction

The dosage ratio of the zymophyte liquid I, the honey and the oat filtrate is 10 mL: 20 g: 250g of prepared fermentation liquor I, fermenting for 12 days at 25-32 ℃, centrifuging for 15min at 8000r/min, and filtering to obtain supernatant, namely the oat extract. Wherein the zymophyte liquid I is Saccharomyces Cerevisiae (Saccharomyces Cerevisiae) in the form of suspension with concentration of 10⁶～10⁸cfu/ml, pH value of 6.5-7.5, and Mel is flos Sophorae Immaturus Mel.

The dosage ratio of the zymophyte liquid II to the honey to the tremella filtrate is 15 mL: 20 g: 100g of fermentation liquor II is prepared. And fermenting the fermentation liquor II at 38 ℃ for 7 days, centrifuging at 8000r/min for 15min, and filtering to obtain supernatant, namely the tremella extract. Wherein the zymocyte liquid II is prepared from Lactobacillus plantarum (Lactobacillus plantarum) and Saccharomyces Cerevisiae (Saccharomyces Cerevisiae) according to the weight ratio of 2: 1, at a concentration of 10⁶～10⁸cfu/ml, the pH value is 6.5-7.5, and the honey is litchi honey.

the using amount ratio of the zymophyte liquid III to the honey to the cactus filtrate is 5 mL: 5 g: 150g of fermentation liquor III is prepared. Fermenting the fermentation liquor III at 26 deg.C for 8 days, centrifuging at 8000r/min for 15min, filtering, and collecting supernatant to obtain the cactus extract. Wherein the zymocyte liquid III is prepared by mixing Lactobacillus delbrueckii, Lactobacillus fermentum and Saccharomyces Cerevisiae in a ratio of 2: 3: 1 composed ofA suspension having a concentration of 10⁶～10⁸cfu/ml, pH value of 6.5-7.5, and Mel is Astragalus sinicus Mel.

The using amount ratio of the zymophyte liquid IV to the honey to the kelp filtrate is 15 mL: 40 g: 200g of fermentation liquor IV is prepared. And fermenting the fermentation liquor IV at 28 ℃ for 10 days, centrifuging at 8000r/min for 15min, and filtering to obtain supernatant, namely the seaweed extract. Wherein the fermentation bacterium liquid IV is prepared by mixing Lactobacillus plantarum (Lactobacillus plantarum), Lactobacillus fermentum (Lactobacillus fermentum) and Lactobacillus acidophilus (Lactobacillus acidophilus) according to the proportion of 1: 1:1, in a concentration of 10⁶～10⁸cfu/ml, the pH value is 6.5-7.5, and the honey is sophora flower honey and jujube flower honey in a ratio of 2: 1.

The above strains were purchased from the culture collection of microorganisms of the institute of microorganisms, Guangdong province.

3) Freeze-drying

mixing the four extracts at a certain proportion, stirring, placing into a freezing bottle, pre-freezing at 4 deg.C for 3 hr, freezing at-10 deg.C for 3 hr, and freezing at-40 deg.C for 6 hr. Taking out, rapidly placing into a precooled freeze dryer, and carrying out sublimation drying for 16 hours under the pressure condition of 20 Pa. And stopping the machine and emptying the machine after the materials are completely dried, and taking out the product to obtain the composition with the moisturizing and relieving effects.

Example 2 composition (II) having moisturizing and soothing effects and preparation method thereof

Compared with example 1, example 2 is prepared in the same manner as example 1 except that the weight parts of the four extracts in the composition are different.

example 3 composition (III) with moisturizing and soothing effects and preparation method thereof

Components	Parts by weight
		Oat extract	30
Tremella extract	40
		Opuntia ficus extract	20
Seaweed extract	10

The preparation method comprises the following steps:

Example 3 compared to example 1, the preparation method was the same as example 1 except that the weight parts of the four extracts in the composition were different.

Example 4 preparation of moisturizing and soothing essence

Table 1 shows the moisturizing essence formula containing the composition of the present invention.

TABLE 1

The preparation method comprises the following steps:

(1) pre-dispersing xanthan gum and sodium hyaluronate in the phase A by using glycerol, putting the pre-dispersed xanthan gum and sodium hyaluronate into an emulsifying pot, directly putting EDTA disodium, glycerol polyacrylate and PEG/PPG/polytetramethylene glycol-8/5/3 glycerol into the emulsifying pot, heating to 80 ℃, stirring and dispersing until the EDTA disodium, the glycerol polyacrylate and the PEG/PPG/polytetramethylene glycol-8/5/3 glycerol are completely dissolved.

(2) the p-hydroxyacetophenone in the phase B is dissolved in butanediol, 1, 3-propanediol and 1, 2-hexanediol by heating in advance until the solution is transparent.

(3) Cooling after the A phase is completely dissolved, adding the B phase when the temperature is reduced to 60 ℃, stirring until the B phase is completely dissolved, cooling to 37 ℃, uniformly stirring and refining, checking physicochemical indexes such as color, pH, fragrance and appearance, vacuumizing and defoaming, filtering with 200 meshes after the inspection is qualified, discharging, and filling.

(4) And (3) independently vacuum-packaging the phase C, and adding the phase C into the prepared phase (3) before use.

Example 5 preparation of moisturizing and soothing essence

The same conditions as in example 4 were used except that the composition (I) having moisturizing and soothing effects was changed to the composition (II) having moisturizing and soothing effects, as compared with example 4.

Example 6 preparation of moisturizing and soothing essence

The same conditions as in example 4 were used except that the composition (I) having moisturizing and soothing effects was changed to the composition (III) having moisturizing and soothing effects as in example 4.

Comparative examples 1-4 preparation of Single extracts

Compared with the example 1, the oat extract, the tremella extract, the cactus extract and the seaweed extract are respectively freeze-dried to prepare single extracts, the comparative examples 1 to 4 are set, and other conditions are the same as the example 1.

Comparative examples 5 to 10 preparation of two Combined extracts

In comparison to example 1, any two of the four extracts were mixed at a ratio of 1: the mass ratio of 1 freeze-dried prepared extracts were set as comparative example 5 (oat + tremella), comparative example 6 (oat + cactus), comparative example 7 (oat + seaweed), comparative example 8 (tremella + cactus), comparative example 9 (tremella + seaweed) and comparative example 10 (cactus + seaweed), and the other conditions were the same as in example 1.

Preparation of three kinds of combination extracts of comparative examples 10 to 14

In comparison to example 1, three of the four extracts were mixed at a ratio of 1: 1:1 (oat + tremella + cactus, oat + tremella + seaweed, oat + cactus + seaweed, tremella + cactus + seaweed) by mass ratio, and the comparative examples 10 to 14 are set, and other conditions are the same as those in example 1.

Comparative examples 15 to 17 preparation of non-lyophilized compositions

The conditions were the same as in examples 1 to 3 except that the composition of the extract was not prepared by lyophilization as in examples 1 to 3, and the composition prepared without lyophilization was set as in comparative examples 15 to 17.

comparative examples 18 to 34 preparation of moisturizing and soothing essence using comparative examples

Compared with example 4, except that the composition (I) with the moisturizing and soothing effects is changed into the compositions of comparative examples 1 to 17, the conditions are the same as example 4, and the moisturizing and soothing essence containing the compositions of comparative examples 1 to 17 is set as comparative examples 18 to 34.

comparative example 35 preparation of general essence

the general essence of the combination (i) for removing moisturizing effect was set as comparative example 35, except that the composition (i) for moisturizing and soothing effects was not added, as compared to example 4, and the conditions were the same as example 4.

EXAMPLE 1 product stability test

The stability test of the product mainly takes heat resistance, cold resistance and freeze-thaw cycle as main tests to verify the stability of the product.

1.1 Heat and Cold resistance test

The heat and cold resistance test is to place the sample in an incubator or refrigerator at 45 ℃ or-18 ℃ respectively, and observe the stability of recovering to room temperature after 1 day, 3 days, 7 days, 15 days, 30 days, 45 days, 60 days, 75 days and 90 days.

1.2 Freeze-thaw cycling test

the main method of the freeze-thaw cycle test is to freeze and thaw the mixture back and forth at the temperature of-5 ℃ and normal temperature, the cycle is carried out for 3 times, and the appearance condition is observed and recorded.

1.3 test results

TABLE 2 stability statistics

The data in Table 2 shows that the addition of the composition to the product system clearly compares the differences between the examples and the comparative examples. The composition which is not processed by the freeze-drying mode is added into the product, has great influence on physical and chemical indexes such as appearance, viscosity and the like of a system, and has very obvious formula stability after being processed by the freeze-drying mode. The compositions of the present invention are shown to have superior stability over conventional actives.

Experimental example 2 testing of skin moisture content and skin moisture loss (TEWL)

The moisture test adopts the recognized methodthe method-capacitance method, TEWL is an important parameter for evaluating the function of the skin moisture protective layer, and has been widely accepted internationally. The more intact the skin protection layer, the higher the moisture content and the lower the value of TEWL for skin moisture loss. The skin moisture test can find out in time whether the protective function of the skin has been destroyed.

2.1 test Instrument

A skin moisture content Corneometer test probe manufactured by Courage + Khazaka, germany (CK, germany). Skin moisture loss (TEWL) TewameterTM300 test probe manufactured by Courage + Khazaka, Germany (CK).

2.2 skin moisture content and TEWL test methods

The following experiments were conducted for examples 4 to 6 and comparative examples 8 to 15.

96 dry skin people (with the cumulative stabbing pain feeling more than or equal to 3 points) are selected, the age is 20-45 years, and the age is half of that of men and women. Groups were randomized into 8 groups of 6 males and 6 females. Any product (cosmetics or external medicines or internal health care products) cannot be used for 15 days before the tested part. Before the test, the subject was asked to wash and cleanse the face and sit still for 30min in a constant temperature and humidity room at a temperature of 22 + -1 deg.C and a humidity of 50 + -5% 2 hours after the washing, and to maintain a relaxed state.

In the experiment, semi-facial tests are carried out according to a random table, wherein one side is examples 4-6, the other side is comparative examples 8-15, the left nasolabial sulcus and the right nasolabial sulcus of a volunteer are selected for carrying out skin moisture content tests and moisture loss (TEWL) tests, and the average value of 3 values is taken as the nasolabial sulcus moisture content data (D0) and the nasolabial sulcus skin moisture loss (TEWL) data (D0). After the test is finished, the volunteers need to use the product according to the requirements, one side face is coated with one product, and the other side face is coated with the other product. Trial was continued for 28 days and required a return visit on day 14 (D14) and day 28 (D28) of the trial.

2.3 results of the experiment

Table 3 moisture content test change rate unit (%)

The results of skin hydration and TEWL are shown by the data in Table 3 to clearly compare the efficacy differences between the examples and the comparative examples. Although the examples and the comparative examples all improve the moisturizing effect of the skin, the improvement effect of the single extract component, the two-two combination and the three-component combination in the product is not ideal, and the data show that the combination of the various extract raw materials in the invention can mutually synergize, so that the effects of improving the skin hydration degree and reducing the skin moisture loss are more obvious than other combination modes, and the moisturizing effect of the extract without freeze-drying on the skin is slightly weaker. The freeze-drying treatment process can ensure the activity of the product and ensure that the moisturizing effect of the product is more excellent.

Experimental example 3 functional evaluation of soothing sensitive stimulation

3.1 evaluation and test method for lactic acid stimulation efficacy of face

In the experiment, a half-face test is carried out according to a random table, wherein one side is example 4-6, the other side is comparative example 8-15, a nasolabial sulcus part of a subject is selected for the experiment, 50ul of a 10% lactic acid solution is dripped on a single-layer filter paper with the diameter of 0.8cm, the subject is asked for stabbing pain feeling at 3min, 4min and 5min respectively, the score is carried out according to a 4-point method (0 is no stabbing pain feeling, 1 is mild stabbing pain, 2 is moderate stabbing pain, and 3 is severe stabbing pain), and the score of a volunteer is recorded as the lactic acid stimulation score of the side (D0). The product is applied as required 2 times a day, and the application is continued for 28 days, and the 14 th (D14) and 28 th (D28) days of the test are required for revisit.

3.2 results of the experiment

Table 4 lactic acid stimulation test results change rate unit (%)

The data in table 4 show that the results of the human half-face test evaluation can clearly compare the efficacy difference between the examples and the comparative examples. Although the examples and the comparative examples have a certain relieving effect on skin sensitivity, the extract composition disclosed by the invention has a very remarkable effect on relieving skin sensitivity, which is obviously higher than the using effect of a single other combination scheme, so that various plant raw materials in the extract composition can be mutually synergistic, the defense function of the skin can be effectively enhanced, the horny layer of the skin can be repaired, and the lactic acid stimulation effect can be reduced; after the active matter is freeze-dried, the activity is enhanced, which shows that the freeze-drying treatment process has good effect of maintaining the activity of the extract.

therefore, the composition with the moisturizing and relieving effects has high stability and long shelf life, and can keep the activity of the effective components to the maximum extent; the composition can be applied to products to improve the skin moisturizing capability, promote the self-hydration, water storage and water distribution of the skin, reduce the water loss of the skin, reduce the skin sensitivity and repair damage and restore the skin to a healthy state.

the above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and all such changes, modifications, substitutions, combinations, and simplifications are intended to be included in the scope of the present invention.

Claims

1. The composition with the effects of moisturizing and relieving is characterized by comprising the following components in parts by weight: 20-50 parts of oat extract, 20-50 parts of tremella extract, 10-30 parts of cactus extract and 5-20 parts of seaweed extract.

2. The composition with the moisturizing and soothing effects as claimed in claim 1, wherein the composition comprises the following components in parts by weight: 30-50 parts of oat extract, 20-40 parts of tremella extract, 10-30 parts of cactus extract and 5-20 parts of seaweed extract.

3. The composition with the moisturizing and soothing effects as claimed in claim 2, wherein the composition comprises the following components in parts by weight: 40-50 parts of oat extract, 20-40 parts of tremella extract, 10-20 parts of cactus extract and 10-20 parts of seaweed extract.

4. the composition with the effects of moisturizing and soothing as claimed in claim 3, which is characterized by comprising the following components in parts by weight: 30 parts of oat extract, 40 parts of tremella extract, 20 parts of cactus extract and 10 parts of seaweed extract.

5. A method for preparing a moisturizing and soothing composition according to any one of claims 1 to 4, comprising the steps of:

(2) Fermentation and extraction: fermenting the filtrate obtained in the step (1) to respectively prepare oat, tremella, cactus and seaweed extracts, specifically comprising the following steps,

(3) Freeze-drying: according to the method in the step (2), the oat extract: white fungus extract: cactus extract: the ratio of the seaweed extract to the seaweed extract is 20-50: mixing and stirring uniformly according to the ratio of 10-30: 5-20, filling the extract mixture into a freezing bottle, and taking out a product after freeze drying to obtain the composition with the effects of moisturizing and relieving.

6. The method for preparing a composition with moisturizing and soothing effects according to claim 5, wherein the filtrate obtained in the step (1) is prepared by the following steps,

7. The method for preparing a composition with moisturizing and soothing effects according to claim 5, wherein in the step (3), the freezing comprises the following specific steps: pre-freezing the mixture extract at 0-4 ℃ for 1-3 h, freezing at-5-10 ℃ for 2-4 h, finally freezing at-40 ℃ for 4-6 h, taking out, rapidly putting into a pre-cooled freeze dryer, carrying out sublimation drying under the pressure of 20-30 Pa for 12-18 h, stopping the machine for emptying after the materials are completely dried, and taking out the product, namely the composition with the effects of moisturizing and relieving.

8. Use of the composition with moisturizing and soothing effects according to any one of claims 1 to 4 in cosmetics, wherein the composition with moisturizing and soothing effects is added in an amount of 0.05-1%.

9. The application of the composition with the effects of moisturizing and soothing in cosmetics according to claim 8, wherein the composition with the effects of moisturizing and soothing is added in an amount of 0.1-0.5%.

10. The use of the composition with moisturizing and soothing effects as claimed in claim 9, wherein the cosmetic comprises at least one of a lotion, an emulsion, a serum, a cream, a mask, a face cleanser, or a BB cream.