CN115125150A - Tricholoma matsutake strain TriMatT35 and application thereof - Google Patents
Tricholoma matsutake strain TriMatT35 and application thereof Download PDFInfo
- Publication number
- CN115125150A CN115125150A CN202210827762.3A CN202210827762A CN115125150A CN 115125150 A CN115125150 A CN 115125150A CN 202210827762 A CN202210827762 A CN 202210827762A CN 115125150 A CN115125150 A CN 115125150A
- Authority
- CN
- China
- Prior art keywords
- tricholoma matsutake
- strain
- hair
- trimatt35
- tricholoma
- Prior art date
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Abstract
The invention relates to the technical field of microorganisms, in particular to a tricholoma matsutake strain TriMatT35 and application thereof. The invention provides a Tricholoma matsutake (Tricholoma matsutake) strain TriMatT35 which is preserved in China center for type culture Collection with the preservation number of CCTCC NO: m20211376. The content of nutrient components such as polysaccharide, amino acid and the like in the strain is obviously improved, especially cysteine. The strain fermentation product has a hair repairing function, can repair the damage of hair scales on the outer part of hair, improve the hydrophily lipophilicity and the combing property of damaged hair, repair the internal structure of the hair, strengthen the hair, and has higher application value in the fields of hair cosmetics and the like.
Description
Technical Field
The invention relates to the technical field of microorganisms, in particular to a tricholoma matsutake strain TriMatT35 and application thereof.
Background
In recent years, more and more people are used for hair dyeing and permanent waving, and the hair dyeing and permanent waving can damage the hair and the scalp, reduce the hair strength and accelerate the aging of the scalp. Aiming at the health problems of the hair and the scalp concerned by people, the development of the hair washing and caring product with the nourishing and caring functions for the hair and the scalp has important significance.
Tricholoma matsutake (Tricholoma matsutake), the scientific name Tricholoma matsutake (Tricholoma matsutake), also known as Armillariella matsutake (matsutake), fungi belonging to Basidiomycotina and Tricholomataceae, is a mycorrhizal fungus grown from trees such as Quercus matsutake (Roxb.) Kuntze. The matsutake has unique and aromatic flavor, is a wild edible and medicinal fungus rich in various nutrient substances and functional active ingredients, and has various physiological functions of resisting tumor, enhancing immunity, controlling blood sugar, reducing cholesterol and blood fat level and the like. In recent years, the growing environment of tricholoma matsutake is seriously damaged due to the damage of forest vegetation and unscientific digging, and the tricholoma matsutake has higher requirements on the growing environment, so that the yield of wild tricholoma matsutake is reduced and the supply is short. At present, the tricholoma matsutake is mainly from wild picking, and because the tricholoma matsutake belongs to living symbiotic bacteria, the artificial culture of the tricholoma matsutake is very difficult.
The matsutake is rich in various nutritional ingredients and can be used for repairing damaged hair, the wild matsutake is high in cost and cannot be applied to the field of hair repair in a large scale, and at present, although some matsutake artificial culture reports exist, the types and the contents of the active ingredients are greatly reduced compared with the wild matsutake, so that the application value of the matsutake is influenced. Therefore, the development of the tricholoma matsutake strain with higher effective component content and excellent growth performance under artificial culture conditions is of great significance for realizing the application of the tricholoma matsutake in the cosmetic fields of hair restoration and the like.
Disclosure of Invention
One of the purposes of the invention is to provide a tricholoma matsutake strain TriMatT 35. Another object of the present invention is to provide a fermented product of the strain and a product containing the strain or the fermented product thereof. The invention also provides the application of the strain and the fermentation product thereof.
In order to solve the problems of the prior art in artificial culture of the tricholoma matsutake strain, the invention aims to develop the tricholoma matsutake strain with excellent growth performance and higher content of functional components in artificial culture, artificially mutates the tricholoma matsutake strain T35, and screens the mutagenized strain with the performance.
The tricholoma matsutake strain T35 has been preserved in China center for type culture Collection (preservation Address: Wuhan university) in 2014 at 25.12 months, with the preservation number of CCTCC NO: m2014671, the strain is disclosed in Chinese patent CN 105039170A.
Through screening, the tricholoma matsutake strain obtained by the invention has better growth performance during artificial culture, and mycelium is rich in functional components such as polysaccharide, amino acid and the like.
Specifically, the invention provides the following technical scheme:
the invention provides a Tricholoma matsutake (Tricholoma matsutake) strain TriMatT35 which is preserved in China center for type culture Collection (CCTCC for short, address: Wuhan, Wuhan university, zip code 430072) in 11 months and 5 days 2021, and the preservation number is CCTCC NO: m20211376, classified and named as Tricholoma matsutake TriMatT 35.
The invention provides a tricholoma matsutake fermentation product which is obtained by fermenting and culturing or fermenting, culturing and extracting tricholoma matsutake strain TriMatT 35.
The above-mentioned fermented product of Tricholoma matsutake may comprise a mixture of one or more of the following (1) to (3):
(1) mycelium of tricholoma matsutake strain TriMatT 35;
(2) a metabolite of tricholoma matsutake strain trimat 35;
(3) composition of the fermentation Medium.
The mycelium of the tricholoma matsutake strain TriMatT35 can be live mycelium or inactivated mycelium.
The metabolites of tricholoma matsutake strain trimat 35 described above may include extracellular metabolites and/or intracellular metabolites. Wherein the intracellular metabolite can be obtained by breaking cell wall of mycelium.
The invention provides a tricholoma matsutake fermentation product, which is a fermentation liquid (without solid-liquid separation, containing thalli and fermentation liquid supernatant) obtained by fermenting and culturing tricholoma matsutake strain TriMatT35, and a supernatant obtained by sterilizing, performing ultrasonic treatment and enzymolysis on the fermentation liquid, and filtering the fermentation liquid.
Preferably, the conditions of the sonication are: the ultrasonic frequency is 15-25KHZ, the power is 140-180W, the temperature is 30-37 ℃, and the time is 30-40 min.
Preferably, the enzymolysis treatment is carried out by using protease, and the preferable enzymolysis conditions are as follows: the consumption of the bromelain is 1000-3000U/g fermentation liquor, the enzymolysis temperature is 30-50 ℃, and the enzymolysis time is 3-4 h.
Specifically, the fermentation product is prepared by a method comprising the following steps:
(1) preparing a tricholoma matsutake seed solution: inoculating Tricholoma matsutake TriMatT35 into liquid seed culture medium, and culturing at 26 deg.C and 120-;
(2) inoculating the pine mushroom seed liquid in the step (1) into a fermentation culture medium according to the inoculation amount of 3-8%, and culturing at 26 ℃, 120-160rpm for 4-6 days to obtain a fermentation liquid;
(3) and (3) sterilizing the fermentation liquor obtained in the step (2), ultrasonically treating the fermentation liquor at the ultrasonic frequency of 15-25KHZ and the power of 140-180W, at the temperature of 30-37 ℃ for 30-40min, then carrying out enzymolysis on the ultrasonically treated fermentation liquor by using protease, wherein the consumption of bromelain is 1000-3000U/g of the fermentation liquor, the enzymolysis temperature is 30-50 ℃ and the enzymolysis time is 3-4h, filtering and collecting supernatant after enzymolysis to obtain the tricholoma matsutake fermentation product.
The invention also provides a microbial inoculum which contains the tricholoma matsutake strain TriMatT35 or the tricholoma matsutake fermentation product, or contains the tricholoma matsutake strain TriMatT35 and the fermentation product thereof.
Among the above-mentioned microbial agents, Tricholoma matsutake strain TriMatT35 may exist in the form of spores or mycelia.
The formulation of the microbial inoculum is not particularly limited, and can be a formulation commonly used in the field of microbial preparations, such as: the solid microbial inoculum comprises a freeze-dried spore powder and the like.
The matsutake is a fungus used as both medicine and food, can be used for preparing various foods and health products, and has medicinal value. The tricholoma matsutake strain TriMatT35 provided by the invention grows faster in the artificial culture process, can harvest a large amount of mycelia in a short time, and meanwhile, the content of functional components such as polysaccharide, amino acid and the like in the mycelia is obviously improved, so that the tricholoma matsutake strain TriMatT35 has higher nutritive value and functional activity.
Based on the functions, the invention provides any one of the following applications of tricholoma matsutake strain trimat 35:
the invention provides an application of tricholoma matsutake strain TriMatT35 or tricholoma matsutake fermentation product or microbial inoculum in preparing food or medicines.
The invention provides application of a tricholoma matsutake strain TriMatT35 or tricholoma matsutake fermentation product or microbial inoculum in preparation of health care products.
The food, the medicine and the health-care product can be products with the functions of resisting oxidation, enhancing immunity, resisting tumors, preventing and treating diabetes, cardiovascular diseases and the like.
The invention also provides application of the tricholoma matsutake strain TriMatT35 or the tricholoma matsutake fermentation product or the microbial inoculum in preparation of cosmetics.
The cosmetics include skin cosmetics, hair cosmetics, beauty cosmetics, etc., wherein the hair cosmetics refer to cosmetics for cleaning, protecting, and nourishing human hair, and include shampoos (shampoos ), conditioners, hair sprays, temporary spray-on hair color (non-hair color) shampoos, hair setting products, hair oils, hair waxes, hair creams, etc.
The tricholoma matsutake strain TriMatT35 has good hair or scalp caring and repairing functions, and can effectively nourish and repair hair and scalp damage caused by perm, hair dyeing, environmental factors and the like.
Based on the application, the invention provides the application of the tricholoma matsutake strain TriMatT35 or the tricholoma matsutake fermentation product or the microbial inoculum in preparing products with functions of maintaining and repairing hair or scalp.
The invention provides a food which contains the tricholoma matsutake strain TriMatT35 or the tricholoma matsutake fermentation product.
The food may further contain other materials (e.g., sweetener, sour agent, etc.) acceptable in the food field.
The invention provides a cosmetic containing the tricholoma matsutake fermentation product.
Preferably, the cosmetic is a hair cosmetic. More preferably, the cosmetic is a shampoo cosmetic.
The shampoo cosmetic comprises tricholoma matsutake fermentation product and shampoo cosmetic base allowed by shampoo cosmetic field.
Preferably, the mass percentage of the tricholoma matsutake fermentation product in the cosmetic is 0.1-15%.
The preparation method of the tricholoma matsutake fermentation product comprises the following steps: and (2) fermenting and culturing the tricholoma matsutake strain TriMatT35 to obtain fermentation liquor, sterilizing, performing ultrasonic treatment and enzymolysis treatment on the fermentation liquor, filtering and collecting supernatant.
Preferably, the above mentioned enzymolysis is protease enzymolysis, the protease used is bromelain, the dosage of the protease is 1000-: the enzymolysis temperature is 30-50 deg.C, and the enzymolysis time is 3-4 h.
The conditions of the ultrasonic treatment described above are preferably: the ultrasonic frequency is 15-25KHZ, the power is 140-180W, the temperature is 30-37 ℃, and the time is 30-40 min.
The conditions for sterilization described above are preferably: sterilizing at the temperature of 115 ℃ and 121 ℃ for 15-25 min.
The medium used in the above-mentioned fermentation culture comprises a carbon source and a nitrogen source. Wherein the carbon source comprises a sugar and the nitrogen source comprises an organic nitrogen source.
Specifically, the carbon source is preferably one or more selected from glucose, ribose, lactose, galactose, fructose, sucrose, maltose, mannose, trehalose, mannitol, and sorbitol. The organic nitrogen source is preferably one or more selected from peptone, beef extract powder and yeast extract powder.
The more suitable carbon-nitrogen source combination mode of the tricholoma matsutake strain TriMatT35 is as follows: the carbon source is glucose, and the nitrogen source is peptone, beef extract powder and yeast extract powder.
Preferred fermentation media comprise the following components (g: ml): 0.5-3% of glucose, 0.5-1.5% of peptone, 0.5-1.5% of beef extract powder and yeast extract0.2 to 0.8 percent of powder, 0.01 to 0.05 percent of dipotassium phosphate, 0.01 to 0.05 percent of magnesium sulfate, V B1 0.005-0.02％。
The fermentation culture conditions are preferably: cultured at 26 ℃ and 160rpm for 4-6 days.
Before the fermentation culture, a step of preparing a seed liquid is also included, and the seed culture medium used preferably comprises the following components (g: ml): 0.5-3% of glucose, 0.5-1.5% of peptone, 0.2-0.8% of yeast extract powder, 0.01-0.05% of dipotassium hydrogen phosphate and 0.01-0.05% of magnesium sulfate.
The seed culture conditions are preferably: culturing at 26 ℃ and 120 ℃ at 160r/min for 4-6 days.
The tricholoma matsutake strain TriMatT35 can be fermented and cultured to obtain mycelium with high content of functional components, and the utilization efficiency of the tricholoma matsutake functional components is improved. The tricholoma matsutake fermentation product prepared by the preparation method can effectively repair damaged hair, and has high application value in hair washing and protecting products.
The invention has the beneficial effects that: the invention provides a tricholoma matsutake strain TriMatT35, which has high genetic stability and does not degenerate after more than 10 subcultures; the strain has good growth performance during artificial culture, can obtain high mycelium yield in a short time, improves the production efficiency of tricholoma matsutake mycelium, and reduces the production cost. Moreover, the mycelium of the tricholoma matsutake strain TriMatT35 contains high-content polysaccharide and amino acid, has high nutritive value and efficacy, particularly has a good hair repairing function, can repair the damage of hair scales on the outside of hair, improve the hydrophile lipophilicity and combing property of damaged hair, repair the internal structure of the hair, strengthen the hair and have high application value in the fields of hair cosmetics and the like.
Drawings
FIG. 1 shows the results of the growth performance test of Tricholoma matsutake Strain TriMatT35 in example 2 of the present invention.
Fig. 2 is a test result of the wet hair combing repairing performance of the shampoo in the experimental example of the invention.
Fig. 3 is a result of testing the hair combing property of shampoo restoration in the experimental example of the present invention.
Detailed Description
The following examples are intended to illustrate the invention, but are not intended to limit the scope of the invention.
Example 1 obtaining of Tricholoma matsutake Strain TriMatT35
The preservation number of the tricholoma matsutake strain is CCTCC NO: m2014671 Tricholoma matsutake strain T35 is an original strain, and the strain is subjected to ultraviolet ray mutagenesis treatment and screening of mutagenized strains, and the specific method comprises the following steps:
(1) the preservation number is CCTCC NO: activating Tricholoma matsutake strain T35M 2014671, inoculating to PDA slant, culturing at 26 deg.C for 5 days, adding phosphate buffer solution with pH of 6.0 into slant culture medium, filtering with 4 layers of gauze, and diluting spore concentration to 10 6 -10 7 seed/mL, used for mutation breeding;
(2) irradiating the spore suspension in the step (1) with 15w of ultraviolet rays for 2min, diluting, coating a flat plate, culturing in a thermostat at 26 ℃ for 2 days, and point-to-point implanting into a 96-well plate filled with a PDA solid culture medium by using a sterilized toothpick for culturing;
inoculating the cultured mutant strains into a 250mL conical flask one by using an inoculating needle for culturing, culturing at 26 ℃ and 150rpm for 5 days to obtain tricholoma matsutake mycelia, respectively measuring the growth condition of the mycelia and the contents of substances such as mycelia polysaccharide, amino acid and the like, and screening the tricholoma matsutake strains with better growth performance and higher content of functional components;
(3) and (3) continuously passaging the obtained tricholoma matsutake mutant strain, culturing for 90-120h in a constant-temperature shaking table at 26 ℃ and 150rpm, measuring mycelium polysaccharide and amino acid, and inspecting the genetic stability of the mutant strain.
Through ultraviolet mutagenesis and screening, the tricholoma matsutake strain with good growth performance and high polysaccharide and amino acid content in mycelium is finally obtained and named as TriMatT 35.
Tricholoma matsutake (Tricholoma matsutake) strain TriMatT35 the strain has been preserved in China center for type culture Collection (CCTCC for short, address: Wuhan, Wuhan university, postal code 430072) in 11 months and 5 days 2021, with the preservation number being CCTCC NO: m20211376, classified and named as Tricholoma matsutake TriMatT 35.
Example 2 Performance testing of Tricholoma matsutake Strain TriMatT35
1. Growth Performance test
Respectively inoculating Tricholoma matsutake strain TriMatT35 and Tricholoma matsutake strain T35 into liquid seed culture medium, respectively culturing for 1, 3 and 5 days at 26 deg.C and 150r/min in a shaking table, measuring mycelium yield, and drawing growth curves of Tricholoma matsutake strains TriMatT35 and T35, wherein the seed culture medium comprises: glucose 2%, peptone 1%, yeast extract 1%, dipotassium hydrogen phosphate 0.02%, magnesium sulfate 0.03%, and V B1 0.01％。
As shown in FIG. 1, it is understood that the growth rate of Tricholoma matsutake TriMatT35 is significantly higher than that of T35, and the yield of mycelia is significantly higher than that of T35.
2. Functional ingredient content detection
The tricholoma matsutake strain TriMatT35, tricholoma matsutake strain T35, and several other tricholoma matsutake strains (named tricholoma matsutake strains 1, 2, 3, and 4) obtained in the mutagenesis screening process of example 1 were inoculated into a liquid seed medium (glucose 2%, peptone 1%, yeast extract 1%, dipotassium hydrogen phosphate 0.02%, magnesium sulfate 0.03%, V) B1 0.01%), shaking at 26 deg.C and 150r/min, culturing for 5 days, centrifuging mycelium at 5000rpm, washing with water for 2 times, lyophilizing mycelium, pulverizing, and sieving with 80 mesh sieve. 0.1g of tricholoma matsutake mycelium dry powder is weighed, 5mL of distilled water is added into each tricholoma matsutake mycelium dry powder, water bath leaching is carried out for 3h at 80 ℃, centrifugation is carried out for 6min at 5000g/min, and the supernatant is taken to measure the contents of polysaccharide, total amino acid and cysteine of the mycelium, and the results are shown in Table 1.
TABLE 1 content of functional ingredients
| Polysaccharide g/g | Amino acid mg/g | Cysteine mg/g | |
| Original strain (T35) | 0.117 | 82 | 12 |
| Tricholoma matsutake strain TriMatT35 | 0.131 | 116 | 33 |
| Tricholoma matsutake Strain 1 | 0.123 | 102 | 23 |
| Tricholoma matsutake Strain 2 | 0.119 | 98 | 21 |
| |
0.116 | 90 | 17 |
| Tricholoma matsutake Strain 4 | 0.118 | 89 | 16 |
3. Genetic stability testing
Inoculating Tricholoma matsutake strain TriMatT35 into liquidIn seed culture medium (glucose 2%, peptone 1%, yeast extract 1%, dipotassium hydrogen phosphate 0.02%, magnesium sulfate 0.03%, V) B1 0.01%), subculture was performed, the contents of polysaccharide and amino acid in the mycelia of the strain were measured every other generation, and the genetic stability of the strain was examined for 10 generations, with the results shown in table 2.
TABLE 2 mutagenized strain stability Studies
EXAMPLE 3 fermentation culture of Tricholoma matsutake Strain TriMatT35 and preparation of fermentation product
Carrying out fermentation culture on tricholoma matsutake strain TriMatT35 and preparing a tricholoma matsutake fermentation product, wherein the specific method comprises the following steps:
(1) preparation method of Tricholoma matsutake seed liquid
Inoculating Tricholoma matsutake strain TriMatT35 into liquid seed culture medium, and culturing at 26 deg.C and 150r/min for 5d to obtain seed solution; the seed culture medium comprises the following components: glucose 2%, peptone 1%, yeast extract powder 1%, dipotassium hydrogen phosphate 0.02%, magnesium sulfate 0.03%, and V B1 0.01％；
(2) Fermentation culture
Inoculating the tricholoma matsutake seed liquid prepared in the step (1) into a fermentation culture medium according to the inoculation amount of 5%, and culturing at 26 ℃ and 150rpm for 5 days to obtain fermentation liquor; the fermentation medium consisted of: 2% of glucose, 1% of peptone, 1% of yeast extract powder, 0.02% of dipotassium hydrogen phosphate and 0.03% of magnesium sulfate;
(3) preparation method of Tricholoma matsutake fermented product
Sterilizing the fermentation liquor obtained in the step (2), sterilizing at 121 ℃ for 20min, then carrying out ultrasonic treatment on the sterilized fermentation liquor at the ultrasonic treatment frequency of 20KHZ, the power of 160W and the temperature of 35 ℃ for 35min, then carrying out enzymolysis on the fermentation liquor subjected to ultrasonic treatment by using bromelin, wherein the use amount of protease is 2600U/g of the fermentation liquor, the enzymolysis temperature is 39 ℃, and the enzymolysis time is 3.6h, filtering, collecting supernatant, and cooling to room temperature to obtain the tricholoma matsutake fermentation product.
Example 4 shampoo containing Tricholoma matsutake Strain TriMatT35 fermentation product
The embodiment provides shampoo, which comprises the following components (by mass): 1% of tricholoma matsutake fermentation product and 99% of shampoo base in example 3.
Wherein, the shampoo matrix comprises the following components (by mass percent): 15% of ammonium laureth sulfate, 3% of sodium methyl myristoyl taurate, 7% of lauramidopropyl betaine, 0.5% of cocamide MEA, 472% of polyquaternary ammonium salt, 0.1% of cocoyl hydrolyzed soybean protein, 0.2% of sodium dilinolamidopropyl PG-dimethyl ammonium chloride phosphate, 0.2% of guar hydroxypropyl trimethyl ammonium chloride, 0.1% of essence, 0.1% of iodopropynyl butyl carbamate and 71.8% of deionized water.
Comparative example 1 shampoo containing fermentation product of Tricholoma matsutake strain T35
The comparative example provides shampoo which comprises the following components in percentage by mass: 1% of T35 tricholoma matsutake fermentation product and 99% of shampoo matrix, wherein the preparation method of the T35 tricholoma matsutake fermentation product is the same as that of the tricholoma matsutake strain TriMatT35 fermentation product in example 3, and the difference is only that the tricholoma matsutake strain TriMatT35 is replaced by the tricholoma matsutake strain T35.
The composition of the shampoo base was the same as that of example 4.
Comparative example 2 shampoo base
This comparative example provides a shampoo base having the same formulation as the shampoo base of example 4.
Experimental example Hair repair function test of Tricholoma matsutake Strain TriMatT35 fermented product
The shampoos of example 4 and comparative example 1 and the shampoo base of comparative example 2 were tested for hair restoration function, and the test methods and test results were as follows:
1. experiment for repairing damaged hair external hair scale damage effect by shampoo
Selecting healthy hair of a volunteer, washing with a sodium dodecyl sulfate solution, rinsing, and naturally drying for later use. Taking 4 parts of original hair, 10g of each part, soaking the original hair for 1h at 30 ℃ by using 0.5g/L NaOH, then taking out the original hair, rinsing the original hair to be neutral, and naturally airing the original hair to prepare damaged hair.
The damaged hair was subjected to a washing treatment using the shampoos of example 4 and comparative example 1 and the shampoo base of comparative example 2, respectively, in an amount of 0.5g for 3min, washed with clean water, and naturally dried.
The hair tresses which are not treated (blank sample) and are washed and treated by shampoo are respectively soaked in 0.2mol/L copper sulfate solution for 15min, and then the adsorption quantity of the hair to copper ions is tested.
TABLE 3 copper ion adsorption amount
Note: p <0.05, P <0.01, P <0.001vs. blank.
When the hair is damaged, the hair is negatively charged to adsorb copper ions, and the damage degree of the hair can be represented by measuring the amount of the copper ions adsorbed by the hair. The more damaged the hair, the more copper ions are adsorbed. As can be seen from table 3, compared with the blank sample, the adsorption amount of copper ions of the hair treated by the shampoo was significantly reduced, while compared with the shampoo containing the tricholoma matsutake strain T35 fermented product, the adsorption amount of copper ions of the hair treated by the shampoo containing the tricholoma matlt 35 fermented product was significantly reduced, which indicates that the tricholoma matsutake strain trimat 35 fermented product has a more significant repairing effect on the damage of the hair scales outside the hair.
2. Experiment for repairing damaged hair by shampoo in hydrophilic and lipophilic properties
Selecting 30 volunteers who are subjected to over-dyeing and ironing within three months, testing the hair contact angle (68-72 degrees for dry hair and 66-70 degrees for wet hair) of each volunteer, randomly dividing the hair into 3 groups, collecting the hair of each volunteer as a blank group, respectively cleaning the damaged hair by using the shampoos of example 4 and comparative example 1 and the shampoo of comparative example 2, using the shampoo water for 3 times every week, collecting the hair of the volunteer once every week, after the experiment of 3 weeks is finished, inserting the hair of each group into water under the two states of dry hair and wet hair by adopting a Wilhelmy balance method, calculating the contact angle of the water on the hair surface according to the force, and obtaining the result shown in Table 4.
TABLE 4 Hair contact Angle
Note: p <0.05, P <0.01, P <0.001vs. blank.
The damage degree of hair is directly related to the hydrophilic and hydrophobic properties of the hair surface, and the larger the damage degree of hair is, the stronger the hydrophilic property is. After the liquid spreads on the solid surface, the solid/liquid interface passes through the angle from the inside of the liquid to the gas/liquid interface, which is called the contact angle. The smaller the contact angle of water on the hair surface, the easier spreading and the stronger hydrophilicity. As shown in table 4, compared with the hair dyed and ironed (blank group), the hair using the shampoo of example 4 has a significantly increased contact angle and a statistical significance (P <0.05), which indicates that the tricholoma matsutake strain trimat 35 leavening can significantly repair the hair damage and restore the lipophilicity of the hair.
3. Effect of shampoo restoration on hair combing
The effect of the shampoo restoration of example 4 on the hair combing performance was analyzed by the following specific method:
30 volunteers who were subjected to over-dyeing and ironing within three months were selected, randomly divided into 3 groups of 10 persons each, and hair of each volunteer was collected before the start of the test and left, and each group of volunteers were washed with the shampoos of example 4 and comparative example 1 and the shampoo base of comparative example 2, respectively, used for 1 week, once every other day, and the hair of the volunteers was collected after the end of the test.
The collected hair was rinsed with tap water, the towel was wiped off the excess water until the hair strands did not drip, combed smoothly with a comb, and the wet combing property was tested with a tensile tester. The test results are shown in fig. 2.
Then, the hair is blown to be half-dry at a position 20cm away from the hair bundle by a blower, combed smoothly by a comb, naturally dried, and the combing property of the dried hair is tested by a universal tension tester. The test results are shown in fig. 3.
The results showed that the shampoo of example 4 was significantly superior in the effect on the combing property (wet combing property and dry combing property) of hair to the shampoo containing the fermented product of tricholoma matsutake strain T35 (comparative example 1) and the shampoo base (comparative example 2).
4. Shampoo repairing, dyeing and ironing damaged hair effect experiment
In order to verify the effect of the shampoo in example 4 on repairing, dyeing and perming damaged hair, 30 volunteers aged 25-60 years, dyed and permed hair within one year and having certain damage to hair during dyeing and perming are randomly divided into 3 groups, 10 people in each group are respectively provided with the shampoo in example 4, the shampoo in comparative example 1 and the shampoo matrix in comparative example 2, the using time is 2 months, the using frequency is once every two days, and the effects of the shampoo on repairing the internal structure of hair and strengthening hair are examined. The volunteers measured the maximum force and work of breaking of the hair before the start of the experiment, and 2 months after shampoo application, the maximum force and work of breaking of the hair were measured, and the change in hair strength was compared. The hair strength was tested using a Diastron tensiometer.
TABLE 5 Hair breaking Strength Change
As shown in table 5, the maximum breaking force and breaking work of the hair were significantly increased after using the shampoo of example 4 compared to before use, indicating that the tricholoma matsutake strain trimat 35 fermented product can significantly repair the internal structure of the hair and improve the hair strength.
Although the invention has been described in detail hereinabove with respect to a general description and specific embodiments thereof, it will be apparent to those skilled in the art that modifications or improvements may be made thereto based on the invention. Accordingly, such modifications and improvements are intended to be within the scope of the invention as claimed.
Claims (10)
1. Tricholoma matsutake (Tricholoma matsutake) strain TriMatT35 is characterized in that the Tricholoma matsutake strain is preserved in China center for type culture Collection with the preservation number of CCTCC NO: m20211376.
2. A fermented product of Tricholoma matsutake (Tricholoma matsutake) according to claim 1, which is obtained by fermentation culture or fermentation culture and extraction of Tricholoma matsutake (Tricholoma matsutake) strain TriMatT 35.
3. Microbial preparation, characterized in that it contains the Tricholoma matsutake (Tricholoma matsutake) strain trimat 35 according to claim 1 and/or the Tricholoma matsutake ferment according to claim 2.
4. Use of Tricholoma matsutake (Tricholoma matsutake) strain trimat 35 according to claim 1 or Tricholoma matsutake fermentation product according to claim 2 or microbial inoculum according to claim 3 for the preparation of a food or a medicament.
5. Use of Tricholoma matsutake (Tricholoma matsutake) strain triatt 35 according to claim 1 or Tricholoma matsutake fermentation product according to claim 2 or microbial inoculum according to claim 3 in the preparation of health care products.
6. Use of Tricholoma matsutake (Tricholoma matsutake) strain trimat 35 according to claim 1 or Tricholoma matsutake fermentation product according to claim 2 or microbial preparation according to claim 3 for the preparation of cosmetics.
7. Use of Tricholoma matsutake (Tricholoma matsutake) strain trimat 35 according to claim 1 or Tricholoma matsutake fermentation product according to claim 2 or microbial inoculum according to claim 3 in the preparation of products with hair or scalp caring and repairing functions.
8. A food product comprising the Tricholoma matsutake (Tricholoma matsutake) strain TriMatT35 of claim 1 or the Tricholoma matsutake fermented product of claim 2.
9. A cosmetic comprising the fermented Tricholoma matsutake Singer of claim 2.
10. The cosmetic according to claim 9, wherein the preparation method of the fermented product comprises: fermenting and culturing the Tricholoma matsutake (Tricholoma matsutake) strain TriMatT35 to obtain fermentation liquor, sterilizing, performing ultrasonic treatment and enzymolysis treatment on the fermentation liquor, filtering, and collecting supernatant.
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