CN112022757B

CN112022757B - Shampoo containing ganoderma lucidum sporophore spore powder fermentation liquid and preparation method thereof

Info

Publication number: CN112022757B
Application number: CN202011052455.XA
Authority: CN
Inventors: 张启清; 盛俊娇; 杨水波; 余海励; 孙绪友
Original assignee: Shanghai Jieshibao Daily Chemical Group Co ltd
Current assignee: Shanghai Jieshibao Daily Chemical Group Co ltd
Priority date: 2020-09-29
Filing date: 2020-09-29
Publication date: 2022-06-21
Anticipated expiration: 2040-09-29
Also published as: CN112022757A

Abstract

The invention provides a shampoo containing ganoderma lucidum sporophore spore powder fermentation liquor. By mixing and fermenting the ganoderma lucidum and the spore powder, the zymophyte hydrolyzes the double-layer cell wall of the ganoderma lucidum spore and the cell wall of the ganoderma lucidum fruiting body, so that intracellular substances flow out, and the extraction of effective components is promoted. Meanwhile, the fermentation can degrade macromolecules to obtain micromolecules with smaller molecular weight, namely, micromolecules which are easier to be absorbed by skin, and the absorption and utilization of the effective substances are promoted. The content of polypeptide is increased by using the ganoderma lucidum sporophore spore powder fermentation liquid, the effects of inhibiting the expression of inflammatory factors, relieving scalp and reducing sensitivity are achieved, and meanwhile, the ganoderma lucidum fermentation liquid is also found to have the effects of regulating scalp microecology, enhancing scalp moisture retention and strengthening scalp barrier.

Description

Shampoo containing ganoderma lucidum sporophore spore powder fermentation liquid and preparation method thereof

Technical Field

The invention relates to the technical field of lightening industry, in particular to a shampoo containing ganoderma lucidum sporophore spore powder fermentation liquor and a preparation method of the shampoo.

Background

With the development of industrialization, air pollution is more and more serious. The hair and scalp are exposed to air and can be adhered with a plurality of pollutants, and the pollutants can irritate the scalp and cause the scalp to be sensitive. On the other hand, the frequency of shampooing is increased, and the shampoo is one of the products frequently used by most modern people. Surfactants such as sulfate and sulfonate added to shampoo have irritation and may also cause scalp sensitivity problems. Therefore, the mildness of the shampoo is an important evaluation index.

Ganoderma is also called LINZHONGLING, and has umbrella shape, pileus kidney shape, semicircular shape or nearly circular shape, and is fruiting body of Ganoderma of Polyporaceae. Ganoderma has long-term bearing, and has effects of nourishing, strengthening body constitution, strengthening body resistance, caring skin, and tranquilizing. Modern researches show that the water extract of the ganoderma lucidum sporocarp also contains water-soluble polysaccharide, protein, amino acid, polypeptide and other substances, and has the effects of resisting radiation, resisting aging, whitening skin, preserving moisture and the like.

The Ganoderma spore powder is spore released during Ganoderma maturation, and is Ganoderma sexual germ cell, also called basidiospore. The ganoderma lucidum spore powder is widely applied to health care products, and the water extract of the ganoderma lucidum spore powder also contains polysaccharide, protein and polypeptide like ganoderma lucidum sporocarp, but the component structure and the content of the ganoderma lucidum spore powder are different. Similarly, the components also have the effects of resisting aging, whitening skin, moisturizing and the like. The analysis and comparison of the components of the ganoderma lucidum sporocarp and the spore powder are carried out in the literature, and the result shows that the protein content of the spore powder is about twice that of the sporocarp, the fat content of the spore powder is five times that of the sporocarp, but the polysaccharide content of the sporocarp is higher. The ganoderma lucidum spore wall has double-layer cell walls, is firm and hard, and only 10 to 20 percent of effective components of the spore powder without wall breaking are absorbed by human bodies.

At present, the fermentation of the ganoderma lucidum is the single fermentation of ganoderma lucidum fruiting bodies or ganoderma lucidum spore powder, and no product formed by mixing and processing the ganoderma lucidum fruiting bodies and the spore powder is available. Actually, the active ingredients contained in the ganoderma lucidum sporocarp and the spore powder are different, and the health-care effect of the product can be enhanced by mixing the two ingredients.

In view of this, the invention is particularly proposed.

Disclosure of Invention

The invention aims to provide shampoo containing ganoderma lucidum sporophore spore powder fermentation liquid, which has the effects of regulating scalp micro-ecology, enhancing scalp moisture retention and strengthening scalp barrier.

The second purpose of the invention is to provide a preparation method of the shampoo.

In order to achieve the purpose, the technical scheme of the invention is as follows:

the invention relates to a shampoo containing ganoderma lucidum sporophore spore powder fermentation liquid, which comprises the following raw materials in parts by weight: 10-15 parts of decyl glucoside, 10-15 parts of disodium cocoyl glutamate, 3-8 parts of sodium methyl cocoyl taurate, 3-8 parts of cocamidopropyl betaine, 0.2-0.6 part of acrylic acid (ester)/C10-30 alkanol acrylate cross-linked polymer, 1-2 parts of propylene glycol laurate, 0.5-1 part of PPG-3 octyl ether, 5-10 parts of ganoderma lucidum sporophore spore powder fermentation liquid, 0.1-0.5 part of dipotassium glycyrrhizinate, 0.1-1 part of aminomethyl propanol, 0.2-0.5 part of piroctone ethanolamine salt, 1-2 parts of PEG-7 glyceryl cocoate, 0.05-0.2 part of argan oil, 0.4-0.8 part of phenoxyethanol, 0.5-1 part of ethylhexyl glycerol, 0.01-0.1 part of citric acid, 0.05-0.1 part of disodium EDTA, 32-66 parts of pure water, preferably, 0.3-0.8 part of essence is also included.

Among them, decyl glucoside is one of novel nonionic surfactants, APG, and has the characteristics of common nonionic and anionic surfactants. The disodium cocoyl glutamate belongs to an amino acid surfactant, has good decontamination and foam increasing capabilities, is mild in components, and has good compatibility with skin. Decyl glucoside and disodium cocoyl glutamate are used together as the primary surfactant in a shampoo. The cocoyl methyl sodium taurate and the cocamidopropyl betaine are used as auxiliary surfactants in the shampoo, and have the functions of foam increasing, thickening and foam stabilizing. Acrylic acid/C10-30 alkanol acrylate crosspolymer is a film former that is useful as a thickener and stabilizer in cosmetic products. Propylene glycol laurate, which has permeation-promoting and surface-active properties, and can be used as an emulsifier or co-emulsifier. The PPG-3 octyl ether is used as a conditioning agent and an ester-forming agent, and has excellent solubilizing, dirt-removing, emulsifying, dispersing, lubricating, wetting, penetrating and foam stabilizing capabilities. The Ganoderma lucidum spore powder fermentation liquid is rich in polypeptide, and has the effects of regulating scalp microecology, enhancing scalp moisture retention, relieving scalp, reducing sensitivity, and strengthening scalp barrier. The dipotassium glycyrrhizinate is one of main extraction components in liquorice, has antioxidant activity, shows strong anti-free radical oxidation effect in a cytochrome P450/NADPH oxidation system, and can resist inflammation and relieve by applying the dipotassium glycyrrhizinate to shampoo. The aminomethyl propanol is mainly used as pH regulator in cosmetics and skin care products. Piroctone olamine salt is an antidandruff agent and has thickening, broad-spectrum antibacterial and deodorant effects. PEG-7 glyceryl cocoate is a hydrophilic emollient ester useful in a surfactant system to supplement oil, maintain oil balance of skin and hair, and reduce dry feel. Argan oil contains a large amount of fatty acids, sterols, vitamin E and phenolic compounds, and can make hair softer, smoother and more shiny. The phenoxyethanol is used as a preservative, and the mass content of the phenoxyethanol in the shampoo is less than 1%. The ethylhexyl glycerin can enhance the antiseptic function of the phenethyl alcohol. Citric acid is one of the fruit acids and has the main effect of accelerating cutin renewal. The disodium EDTA is used as a chelating agent, has a stabilizing effect in shampoo, and has a certain synergistic effect on an antiseptic system and an antioxidant system.

The invention also relates to a preparation method of the shampoo, which comprises the following steps:

(1) 4-6 parts of pure water and acrylic acid (ester)/C10-30 alkanol acrylate cross-linked polymer are uniformly mixed to obtain phase A for later use;

uniformly mixing 28-60 parts of pure water, disodium EDTA (ethylene diamine tetraacetic acid), decyl glucoside, disodium cocoyl glutamate, sodium methyl cocoyl taurate, cocamidopropyl betaine, propylene glycol laurate and PPG-3 octyl ether to obtain a phase B for later use;

uniformly mixing PEG-7 glyceryl cocoate, Ganoderma sporophore spore powder fermentation broth, argan oil and essence to obtain phase C;

uniformly mixing piroctone olamine salt and dipotassium glycyrrhizinate to obtain a phase D for later use;

uniformly mixing phenoxyethanol and ethylhexyl glycerol to obtain phase E for later use;

uniformly mixing the aminomethyl propanol and the citric acid to obtain a phase F for later use;

(2) heating the phase A to 75-80 ℃ in a stirring pot under the stirring condition, and stirring until the phase A is uniformly mixed;

(3) adding the phase B into a stirring pot, stirring for 20-30min under heat preservation, and then cooling;

(4) dissolving phase C uniformly in advance, adding phase C into a stirring pot when the temperature in the stirring pot is reduced to 40-45 ℃, and stirring uniformly;

(5) stopping heating, adding the phase D component into a stirring pot, and stirring until the phase D component is dissolved uniformly;

(6) adding the phase E and the phase F into a stirring pot, uniformly stirring, adjusting the pH value to 6-6.5, and discharging.

The mechanism of the preparation process is explained as follows: 1) the phase A and the phase B are water-soluble substances, and can be dissolved at a high temperature of 75-80 ℃ due to the characteristics of raw materials. Because the surfactant in the B phase is more in variety, the B phase is added after the A phase, and the B phase is easy to agglomerate when being added into the preheated water phase independently, and the B phase is difficult to be dissolved when being added into the preheated water phase as the A phase. 2) Both phase C and phase D contain bioactive substances and are added after lowering the temperature. 3) Phase E is a preservative, and phase F components are added finally to adjust the pH value.

The invention also relates to a preparation method of the ganoderma lucidum sporophore spore powder fermentation liquor, which specifically comprises the following steps:

(1) preparing a lucid ganoderma fermentation substrate: drying Ganoderma fruiting body, pulverizing, sieving with 80-120 mesh sieve, adding spore powder, adding distilled water to obtain solution with mass fraction of 7-13%, and sterilizing to obtain sterilized Ganoderma fermentation substrate;

(2) activating strains: respectively inoculating the strains into culture medium by plate streaking method, and culturing at 30-37 deg.C for 44-52 hr to obtain activated strains;

(3) mixing and fermenting: carrying out amplification culture on the activated strains, mixing to obtain a mixed seed solution, adding the mixed seed solution into a sterilized ganoderma lucidum fermentation substrate, and fermenting to obtain an initial ganoderma lucidum sporophore spore powder fermentation liquid;

(4) and (3) fermentation post-treatment: sterilizing the original ganoderma lucidum sporophore spore powder fermentation liquor, crushing thalli, and filtering to obtain the ganoderma lucidum sporophore spore powder fermentation liquor.

Further, the mass ratio of the ganoderma lucidum fruiting body to the spore powder in the step (1) is (10-1): (1-10).

Further, the mass ratio of the ganoderma lucidum fruiting body to the spore powder in the step (1) is 1: 1.

The inventor finds that the content of each active ingredient, particularly the content of polypeptide, in the fermentation liquor obtained by fermenting the ganoderma lucidum sporocarp and the spore powder according to the mass ratio is high through a large number of experiments.

Further, the strains in the step (2) are selected from one or more of bifidobacterium adolescentis CICC 6175, bacillus natto CICC 10262, lactobacillus bulgaricus CICC 20271 and saccharomyces cerevisiae CICC 1252.

The bifidobacterium adolescentis CICC 6175, the bacillus natto CICC 10262, the lactobacillus bulgaricus CICC 20271 and the saccharomyces cerevisiae CICC 1252 are all purchased from China industrial microorganism strain preservation management center.

Further, the strain is selected from mixed strains of bifidobacterium adolescentis CICC 6175, bacillus natto CICC 10262, lactobacillus bulgaricus CICC 20271 and saccharomyces cerevisiae CICC 1252.

Further, the mass ratio of the bifidobacterium adolescentis CICC 6175, the bacillus natto CICC 10262, the lactobacillus bulgaricus CICC 20271 and the saccharomyces cerevisiae CICC 1252 in the mixed seed liquid in the step (3) is (1.5-2.5) to (1.5: -2.5): (0.8-1.2).

Further, in the step (3), the mass ratio of the bifidobacterium adolescentis CICC 6175, the bacillus natto CICC 10262, the lactobacillus bulgaricus CICC 20271 and the saccharomyces cerevisiae CICC 1252 in the mixed seed liquid is 2:1:2: 1.

Through a large number of experiments, the inventor discovers that when the strains are mixed and fermented by the four strains, tough double-layer cell walls of spore powder and cell walls of sporocarp can be opened, cell membranes are broken, intracellular components flow out, the utilization rate of active substances is improved, and the moisture-keeping and allergy-relieving effects of the shampoo can be improved. Meanwhile, the four strains in the proportion range are adopted for fermentation, so that the extraction rate of beneficial components in the ganoderma lucidum fruiting body and the spore powder is high.

The activation method of each strain in the invention is carried out by adopting the existing method, and the method comprises the following steps:

activating culture medium of bifidobacterium adolescentis: MRS culture medium, beef extract 5g/L, peptone 10g/L, glucose 20g/L, yeast powder 4g/L, sodium acetate 5g/L, magnesium sulfate 0.2g/L, triammonium citrate 2g/L, dipotassium hydrogen phosphate 2g/L, manganese sulfate 0.05g/L, Tween-801 g/L, agar 20g/L, distilled water, pH value 6.2 +/-0.2;

the method for activating the bifidobacterium adolescentis comprises the following steps: streaking in MRS solid culture medium, anaerobic culturing at 37 deg.C for 48 hr, and anaerobic culturing for 3 generations.

Activating culture medium of bacillus natto: beef extract peptone culture medium, 3g/L beef extract, 10g/L peptone, 5g/L NaCl, 15-20 g/L agar, distilled water and pH value of 7.5 +/-0.2;

the activation method of the bacillus natto comprises the following steps: streaking on beef extract peptone medium, and culturing at 35 deg.C for 48 hr.

Lactobacillus bulgaricus activation medium: MRS solid culture medium, beef extract 5g/L, peptone 10g/L, glucose 20g/L, yeast powder 4g/L, sodium acetate 5g/L, magnesium sulfate 0.2g/L, triammonium citrate 2g/L, dipotassium hydrogen phosphate 2g/L, manganese sulfate 0.05g/L, Tween-801 g/L, agar 20g/L, distilled water, pH value 6.2 +/-0.2;

the lactobacillus bulgaricus activation method comprises the following steps: streaking in MRS culture medium, and anaerobic culturing at 37 deg.C for 48 hr.

The activation culture medium of saccharomyces cerevisiae: a nutrient yeast glucose liquid culture medium (NYDA solid culture medium), beef extract g/L, yeast extract powder 5g/L, glucose 10g/L, agar 20g/L and normal PH.

The saccharomyces cerevisiae activation method comprises the following steps: streaking on NYDA medium, and culturing at 30 deg.C for 48 hr.

Further, the mixed seed liquid in the step (3) is added into the sterilized ganoderma lucidum fermentation substrate by the mass fraction of 1.5-2.5%.

Further, the fermentation temperature in the step (3) is 35-45 ℃, and the fermentation is carried out for 44-52h on a shaking table with the rotation speed of 100-.

The amplification culture of the activated strain is specifically as follows:

each medium was a liquid medium:

the saccharomyces cerevisiae CICC 1252 amplification culture medium comprises: NYDB liquid culture medium, beef extract g/L, yeast extract powder 5g/L, glucose 10g/L, agar 20g/L, and normal pH.

After activation, selecting a bacterial colony from the bacterial colony of the solid culture medium by using an inoculating needle or an inoculating loop, putting the bacterial colony into a triangular flask filled with a liquid culture medium, slightly swinging the inoculating needle to disperse the thalli in the liquid culture medium, culturing at 30 ℃ and 120rpm until the strain number reaches 1 x 10^8cfu/mL, and obtaining the saccharomyces cerevisiae seed solution.

Bacillus natto CICC 10262 enlarged culture medium: 3g/L beef extract, 10g/L peptone, 5g/L NaCl and 7 PH.

After the solid culture medium is activated, selecting a bacterial colony from the bacterial colony of the solid culture medium by using an inoculating needle or an inoculating loop, putting the bacterial colony into a triangular flask filled with a liquid culture medium, slightly swinging the inoculating needle to disperse the thallus into the liquid culture medium, culturing at 35 ℃ and 120rpm until the bacterial strain number reaches 1 x 10^8cfu/mL, and obtaining the bacillus natto seed solution.

Bifidobacterium adolescentis CICC 6175 culture medium: the MRS liquid culture medium comprises 5g/L beef extract, 10g/L peptone, 20g/L glucose, 4g/L yeast powder, 5g/L sodium acetate, 0.2g/L magnesium sulfate, 2g/L triammonium citrate, 2g/L dipotassium hydrogen phosphate, 0.05g/L manganese sulfate, 801 g/L tween-801 g/L and the pH value of 6.2 +/-0.2.

After the solid culture medium is activated, selecting bacterial colonies from the bacterial colonies of the solid culture medium by using an inoculating needle or an inoculating loop, putting the bacterial colonies into a triangular flask filled with a liquid culture medium, and standing and culturing for 24 hours; absorbing the bacterial liquid in the previous step, and inoculating the bacterial liquid into a triangular flask filled with a liquid culture medium, wherein the inoculum size is 5 percent, and the bacterial liquid is cultured for 12 hours at 37 ℃ and 20 rpm; absorbing the bacterial liquid in the previous step, and inoculating the bacterial liquid into a triangular flask filled with a liquid culture medium, wherein the inoculation amount is 10 percent, and the bacterial liquid is cultured at 37 ℃ and 60rpm for 12 hours; sucking the bacterial liquid in the previous step, inoculating the bacterial liquid into a triangular flask filled with a liquid culture medium, culturing at 37 ℃ and 120rpm until the bacterial quantity reaches 1 x 10^8cfu/mL, and obtaining a bifidobacterium adolescentis seed liquid;

lactobacillus bulgaricus CICC 20271 (aerotolerant training):

after the solid culture medium is activated, selecting a bacterial colony from the bacterial colony of the solid culture medium by using an inoculating needle or an inoculating loop, putting the bacterial colony into a triangular flask filled with a liquid culture medium, and standing and culturing for 24 hours; absorbing the bacterial liquid in the previous step, and inoculating the bacterial liquid into a triangular flask filled with a liquid culture medium, wherein the inoculation amount is 5 percent, and the bacterial liquid is cultured at 37 ℃ and 20rpm for 12 hours; absorbing the bacterial liquid in the previous step, and inoculating the bacterial liquid into a triangular flask filled with a liquid culture medium, wherein the inoculation amount is 10 percent, and the bacterial liquid is cultured at 37 ℃ and 60rpm for 12 hours; sucking the bacterial liquid in the previous step, inoculating the bacterial liquid into a triangular flask filled with a liquid culture medium, culturing at 37 ℃ and 120rpm until the bacterial quantity reaches 1 x 10^8cfu/mL, and obtaining a lactobacillus bulgaricus seed liquid;

or inoculating 5% of the activated liquid culture medium into a triangular flask filled with the liquid culture medium, and standing and culturing for 24 h; absorbing the bacterial liquid in the previous step, and inoculating the bacterial liquid into a triangular flask filled with a liquid culture medium, wherein the inoculation amount is 5 percent, and the bacterial liquid is cultured at 37 ℃ and 20rpm for 12 hours; absorbing the bacterial liquid in the previous step, and inoculating the bacterial liquid into a triangular flask filled with a liquid culture medium, wherein the inoculation amount is 10 percent, and the bacterial liquid is cultured at 37 ℃ and 60rpm for 12 hours; and (3) sucking the bacterial liquid in the previous step, inoculating the bacterial liquid into a triangular flask filled with a liquid culture medium, culturing at 37 ℃ and 120rpm until the bacterial quantity reaches 1 x 10^9cfu/mL, and obtaining the lactobacillus bulgaricus seed liquid.

Further, the sterilization treatment in the steps (1) and (4) is sterilization at 121 ℃ for 15min or sterilization at 600MPa and 25 ℃ under ultrahigh pressure.

Further, the thallus is crushed by a high-pressure homogenizer or ultrasonic crushing in the step (4), and the filtering is performed by centrifugation, an ultrafiltration membrane, a microfiltration membrane or reverse osmosis filtration.

Through a large number of experiments, the inventor finds that after the thalli are broken, partial substances in the lucid ganoderma cells are dissolved out, and beneficial ingredients flow out. A High Pressure Homogenizer was used, the Pressure being set at 100mpa, the homogenization being carried out 3 times and the exit temperature being 25 ℃.

The invention has the beneficial effects that:

In a preferred embodiment of the present invention, the shampoo is mild in nature and non-irritating by adding decyl glucoside, disodium cocoyl glutamate, sodium methylcocoyl taurate and cocamidopropyl betaine to the shampoo as surfactants.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the technical solutions of the present invention will be described in detail below. It is to be understood that the described embodiments are merely exemplary of the invention, and not restrictive of the full scope of the invention. All other embodiments, which can be derived by a person skilled in the art from the examples given herein without making any creative effort, shall fall within the protection scope of the present invention.

The specific strain activation method in the examples is as follows:

Saccharomyces cerevisiae activation medium: a nutrient yeast glucose liquid culture medium (NYDA solid culture medium), beef extract g/L, yeast extract powder 5g/L, glucose 10g/L, agar 20g/L and normal pH.

The activation method of the saccharomyces cerevisiae comprises the following steps: streaking on NYDA medium, and culturing at 30 deg.C for 48 hr.

The activated strain is specifically cultured as follows (each culture medium is a liquid culture medium):

The bacillus natto CICC 10262 enlarged culture medium: 3g/L beef extract, 10g/L peptone, 5g/L NaCl and pH 7.

After the solid culture medium is activated, selecting a bacterial colony from the bacterial colony of the solid culture medium by using an inoculating needle or an inoculating loop, putting the bacterial colony into a triangular flask filled with a liquid culture medium, slightly swinging the inoculating needle to disperse the thallus into the liquid culture medium, culturing at 35 ℃ and 120rpm until the number of strains reaches 1 × 10^8cfu/mL, and obtaining the bacillus natto seed solution.

After the solid culture medium is activated, selecting a bacterial colony from the bacterial colony of the solid culture medium by using an inoculating needle or an inoculating loop, putting the bacterial colony into a triangular flask filled with a liquid culture medium, and standing and culturing for 24 hours; absorbing the bacterial liquid in the previous step, and inoculating the bacterial liquid into a triangular flask filled with a liquid culture medium, wherein the inoculation amount is 5 percent, and the bacterial liquid is cultured at 37 ℃ and 20rpm for 12 hours; absorbing the bacterial liquid in the previous step, and inoculating the bacterial liquid into a triangular flask filled with a liquid culture medium, wherein the inoculation amount is 10 percent, and the bacterial liquid is cultured at 37 ℃ and 60rpm for 12 hours; sucking the bacterial liquid in the previous step, inoculating the bacterial liquid into a triangular flask filled with a liquid culture medium, culturing at 37 ℃ and 120rpm until the bacterial quantity reaches 1 x 10^8cfu/mL, and obtaining a bifidobacterium adolescentis seed liquid;

lactobacillus bulgaricus CICC 20271 (aerotolerant training):

after the solid culture medium is activated, selecting a bacterial colony from the bacterial colony of the solid culture medium by using an inoculating needle or an inoculating loop, putting the bacterial colony into a triangular flask filled with a liquid culture medium, and standing and culturing for 24 hours; absorbing the bacterial liquid in the previous step, and inoculating the bacterial liquid into a triangular flask filled with a liquid culture medium, wherein the inoculum size is 5 percent, and the bacterial liquid is cultured for 12 hours at 37 ℃ and 20 rpm; absorbing the bacterial liquid in the previous step, and inoculating the bacterial liquid into a triangular flask filled with a liquid culture medium, wherein the inoculation amount is 10 percent, and the bacterial liquid is cultured at 37 ℃ and 60rpm for 12 hours; and (3) sucking the bacterial liquid in the previous step, inoculating the bacterial liquid into a triangular flask filled with a liquid culture medium, culturing at 37 ℃ and 120rpm until the bacterial quantity reaches 1 x 10^8cfu/mL, and obtaining the lactobacillus bulgaricus seed liquid.

The preparation example is the preparation of fermentation liquor of spore powder of Ganoderma lucidum fruiting body

Preparation example 1

The preparation method of the ganoderma lucidum sporophore spore powder fermentation liquor comprises the following steps:

(1) preparing a lucid ganoderma fermentation substrate: drying Ganoderma encarpium, pulverizing, sieving with 100 mesh sieve, adding spore powder at a mass ratio of Ganoderma encarpium to spore powder of 1:1, adding distilled water to prepare into 10% solution, sterilizing at 121 deg.C for 15min to obtain sterilized Ganoderma fermentation substrate;

(2) activating strains: respectively inoculating strains into a culture medium for culture by adopting a plate scribing method, wherein the strains are mixed strains of bifidobacterium adolescentis CICC 6175, bacillus natto CICC 10262, lactobacillus bulgaricus CICC 20271 and saccharomyces cerevisiae CICC 1252 to obtain activated strains;

(3) mixing and fermenting: carrying out amplification culture on the obtained activated strains, mixing to obtain a mixed seed solution, adding 2% of the mixed seed solution into a sterilized ganoderma lucidum fermentation substrate according to the mass ratio of 2:1:2:1 of bifidobacterium adolescentis CICC 6175, bacillus natto CICC 10262, lactobacillus bulgaricus CICC 20271 and saccharomyces cerevisiae CICC 1252, fermenting at the fermentation temperature of 40 ℃ for 48h on a shaking table with the rotation speed of 125rpm to obtain an initial ganoderma lucidum sporophore spore powder fermentation liquid;

(4) and (3) fermentation post-treatment: sterilizing the original Ganoderma sporophore spore powder fermentation liquid at 121 deg.C for 15min, crushing thallus with high pressure homogenizer at pressure of 100mpa, homogenizing for 3 times at outlet temperature of 25 deg.C, and centrifuging and filtering to obtain Ganoderma sporophore spore powder fermentation liquid.

In the following preparation examples, one or more experimental parameters were changed, and the other operations were the same as in preparation example 1, and the specific parameter settings are shown in table 1, and bifidobacterium adolescentis CICC 6175, bacillus natto CICC 10262, lactobacillus bulgaricus CICC 20271, and saccharomyces cerevisiae CICC 1252 in the preparation examples were purchased from the china industrial culture collection center.

Comparative example 1

The preparation method of the ganoderma lucidum sporophore spore powder fermentation liquid in the comparative example comprises the following steps: crushing the ganoderma lucidum fruiting body, sieving the crushed ganoderma lucidum fruiting body with a 100-mesh sieve, adding spore powder, wherein the mass ratio of the ganoderma lucidum fruiting body to the spore powder is 1:1, adding water to prepare a solution with the mass fraction of 10%, sterilizing at 121 ℃ for 15min, then preserving heat at 40 ℃ for 48h, sterilizing at 121 ℃ for 15min, and centrifuging to obtain a supernatant, thus obtaining a clear water extracting solution.

Comparative example 2

The preparation method of the fermentation liquid of the spore powder of the ganoderma lucidum fruiting body in the comparative example is the same as that of the preparation example 1, except that the spore powder and the ganoderma lucidum fruiting body are replaced by the ganoderma lucidum fruiting body with the same weight for fermentation.

Comparative example 3

The preparation method of the fermentation solution of the spore powder of the ganoderma lucidum fruiting body in the comparative example is the same as that of the preparation example 1, except that the spore powder and the ganoderma lucidum fruiting body are replaced by the spore powder with the same weight for fermentation.

Comparative example 4

The method for preparing the fermentation liquid of the spore powder of the fruiting body of Ganoderma lucidum in the present comparative example is the same as that of preparation example 1, except that the thallus is not crushed in step (4).

TABLE 1

Measurement of fermentation liquor components of ganoderma lucidum sporophore spore powder

Respectively measuring the fermentation liquor of the spore powder of the ganoderma lucidum fruiting body prepared in the preparation examples 1-14 and the fermentation liquor of the spore powder of the ganoderma lucidum fruiting body prepared in the comparative examples 1-4, and referring to SNT4260 in the polysaccharide test method; determining the content of protein polypeptide by using a BCA method; determining polyphenol content by Folin-Ciocalteu method; using Al (NO)₃)₃-NaNO₂The total flavone content was measured by NaOH method and the results are shown in Table 2.

TABLE 2

As can be seen from the preparation examples 1-5 in tables 1 and 2, the mass ratio of the mixed seed liquid of the invention to the bifidobacterium adolescentis CICC 6175, the bacillus natto CICC 10262, the lactobacillus bulgaricus CICC 20271 and the saccharomyces cerevisiae CICC 1252 is (1.5-2.5) to 1 (1.5: -2.5): (0.8-1.2), the fermentation liquor of the ganoderma lucidum spore powder has relatively high contents of polysaccharide, polypeptide, polyphenol and total flavone, and when the ratio is 2:1:2:1, namely the content of each component is the highest in the scheme of the preparation example 1, which shows that the extraction rate of the beneficial components in the ganoderma lucidum spore powder and the ganoderma lucidum sporocarp is the highest when the four strains are fermented in the ratio range.

As can be seen from the data in preparation examples 1-3 and preparation examples 6-8, the fermentation using four species of bacteria had higher levels of beneficial ingredients in the fermentation broth than the fermentation broth in the fruiting body and spore powder of Ganoderma lucidum using less than four species of bacteria.

As is clear from the data of preparation example 1 and preparation examples 9 to 12, the ratio of spore powder to fruit body according to the present invention was 1: the beneficial ingredients in the fermentation liquor in the ganoderma lucidum fruiting body and the spore powder obtained by fermentation under 1 st are high.

As can be seen from the data of comparative examples 1-4, the fermentation liquor prepared by preparing the water extract of the ganoderma lucidum fruiting body and the spore powder by water extraction and fermenting the spore powder or the ganoderma lucidum fruiting body independently has lower beneficial ingredients, and the content of the beneficial ingredients in the fermentation liquor can be obviously improved by crushing the thalli obtained in the step (4).

Bacteriostatic experiment of ganoderma lucidum fermentation liquor

(1) Staphylococcus epidermidis ATCC12228 was inoculated in nutrient broth, Staphylococcus aureus ATCC6538 was inoculated in nutrient broth, Propionibacterium acnes ATCC6919 was inoculated in GAM culture solution, and Malassezia furfur ATCC44344 was cultured in olive oil medium.

(2) 10mL of each culture solution and 2 parts of each culture solution are respectively added with 0.1mL of the ganoderma lucidum fermentation liquid of the preparation example 1 and 0.1mL of deionized water as blank controls.

(3) Aerobic culture of Staphylococcus epidermidis and Staphylococcus aureus at 37 deg.C for 24 hr, and anaerobic culture of Propionibacterium acnes and Malassezia furfur at 37 deg.C for 48 hr.

(4) The number of each bacterium in the culture solution before and after the culture was measured by the dilution plate method, and the results of the measurement are shown in Table 3.

TABLE 3

As can be seen from the data in Table 3, the amount of Staphylococcus aureus decreased by 1 order of magnitude after 24h of incubation with the Ganoderma lucidum fermentation broth, the amount of Malassezia furfur was also suppressed, and the amounts of Propionibacterium acnes and Staphylococcus epidermidis were not significantly changed. The ganoderma lucidum fermentation liquor can act on scalp microorganisms, and particularly can selectively inhibit staphylococcus aureus and malassezia furfur so as to balance scalp flora. The shampoo can regulate scalp microecology, and has effects of removing dandruff and nourishing scalp.

The present inventors also conducted the above-mentioned experiments on preparation examples 2 to 3, preparation examples 6 to 10, and preparation examples 13 to 14, and the results were substantially consistent and not listed one by one due to space limitations.

Anti-inflammatory data

1. Experimental methods

RAW264.7 cells were seeded in a 96-well plate, induced with LPS at 1. mu.g/ml and simultaneously treated with a test compound (final concentration 50. mu.M) to set a drug-free group and an L-NMMA positive drug group as controls. After overnight incubation, the cells were incubated in medium for NO production and absorbance was measured at 570 nm. MTS was added to the remaining medium for cell viability assays to exclude the toxic effects of the compound on the cells.

Inhibition rate (%) of NO production (non-drug-treated group OD)_570nmSample set OD_570nm) Non-drug treatment group OD_570nm×100％

IC50 (50% concentration of inhibition) was calculated according to the Reed & Muench method.

2. Reagent

Mouse mononuclear macrophage RAW264.7 was purchased from shanghai cell bank of chinese academy, DMEM medium and fetal bovine serum from BI company. Griess Reagent, LPS and control drug L-NMMA were purchased from Sigma.

3. Results of the experiment

TABLE 4

Sample (I)	Concentration (μ M)	NO production inhibition (%)
			L-NMMA	50	53.59±1.89
Preparation example 1 Ganoderma lucidum spore powder fermentation broth	50	51.74±2.70
			Preparation example 2 fermentation broth of spore powder of Ganoderma lucidum fruiting body	50	49.13±2.26
Preparation example 3 fermentation broth of spore powder of Ganoderma lucidum fruiting body	50	48.56±2.05
			Preparation example 4 Ganoderma lucidum spore powder fermentation broth	50	46.28±1.81
Preparation example 5 fermentation broth of spore powder of Ganoderma lucidum fruiting body	50	46.37±1.95
			Preparation example 6 fermentation broth of spore powder of Ganoderma lucidum fruiting body	50	47.03±2.59
Preparation example 7 fermentation broth of spore powder of Ganoderma lucidum fruiting body	50	47.25±2.16
			Preparation example 8 fermentation broth of spore powder of Ganoderma lucidum fruiting body	50	46.55±1.76
Preparation example 9 fermentation broth of spore powder of Ganoderma lucidum fruiting body	50	48.92±2.11
			Preparation example 10 fermentation broth of spore powder of Ganoderma lucidum fruiting body	50	48.32±2.14
Preparation example 11 Ganoderma lucidum spore powder fermentation broth	50	42.24±1.59
			Preparation example 12 fermentation broth of spore powder of Ganoderma lucidum fruiting body	50	41.21±2.25
Preparation example 13 fermentation broth of spore powder of Ganoderma lucidum fruiting body	50	49.52±2.34
			Preparation example 14 fermentation broth of spore powder of fruiting body of Ganoderma lucidum	50	49.74±2.52
Comparative example 1 Ganoderma lucidum spore powder fermentation broth	50	32.56±2.41
			Comparative example 2 Ganoderma lucidum spore powder fermentation broth	50	40.58±1.59
Comparative example 3 Ganoderma lucidum spore powder fermentation broth	50	43.25±1.86
			Comparative example 4 Ganoderma lucidum spore powder fermentation broth	50	45.89±2.21

4. Analysis of results

Nitric Oxide (NO) has a wide range of important biological control functions and plays an important role in inflammation, tumors, cardiovascular systems and the like. When immune cells are stimulated by microbial endotoxins, inflammatory mediators, etc., a large amount of Inducible Nitric Oxide Synthase (iNOS) is produced to produce NO for an immune response, and thus inhibition of NO production is a direct indicator of the anti-inflammatory activity of a compound. Inducing generation of nitric oxide synthase by using LPS (lipopolysaccharide) lipopolysaccharide on mouse mononuclear macrophage RAW264.7, simultaneously adding a compound to be detected for treatment, and absorbing a culture medium to detect a Nitrite (NO) by measuring an absorbance value at a wavelength of 570nm through a Griess method₂ ^-)。

As can be seen from the above table, the fermentation broth of spore powder of Ganoderma lucidum fruiting body prepared by the method of the present invention can inhibit the generation of nitric oxide and does not produce cytotoxicity, and the data of examples 1-12 show that when the mass ratio of Ganoderma lucidum fruiting body to spore powder is not (10-1): (1-10), the nitrogen monoxide inhibition rate is obviously reduced, and the data of the example 1 and the comparative examples 1-4 show that the nitrogen monoxide inhibition rate of the fermentation liquor prepared by preparing the ganoderma lucidum sporocarp and the spore powder water extract by water extraction and fermenting the spore powder or the ganoderma lucidum sporocarp alone is obviously reduced, so that the ganoderma lucidum fermentation liquor prepared by the invention has better anti-inflammatory effect, and the anti-inflammatory effect is obviously related to the specific fermentation method of the invention, including various parameters including strain selection, the ratio between strains and the mass ratio of the ganoderma lucidum sporocarp and the spore powder.

Example preparation of shampoo

Example 1

(1) 4 parts of pure water and 0.2 part of acrylic acid (ester)/C10-30 alkanol acrylate cross-linked polymer are uniformly mixed to obtain phase A for later use;

59.84 parts of pure water, 0.05 part of disodium EDTA, 10 parts of decyl glucoside, 10 parts of disodium cocoyl glutamate, 3 parts of sodium methyl cocoyl taurate, 3 parts of cocamidopropyl betaine, 1 part of propylene glycol laurate and 0.5 part of PPG-3 octyl ether are uniformly mixed to obtain a phase B for later use;

uniformly mixing 1 part of PEG-7 glyceryl cocoate, 5 parts of ganoderma lucidum sporophore spore powder fermentation liquor of preparation example 1 and 0.05 part of argan oil to obtain a C phase for later use;

uniformly mixing 0.2 part of piroctone olamine salt and 0.1 part of dipotassium glycyrrhizinate to obtain a phase D for later use;

uniformly mixing 0.4 part of phenoxyethanol and 0.5 part of ethylhexyl glycerol to obtain an E phase for later use;

uniformly mixing 0.1 part of aminomethyl propanol and 0.01 part of citric acid to obtain a phase F for later use;

(2) heating the phase A to 80 ℃ in a stirring pot under the stirring condition, and stirring until the phase A is uniformly mixed;

(3) adding the phase B into a stirring pot, keeping the temperature and stirring for 25min, and then cooling;

The experimental parameters of examples 2 to 5 and comparative examples 5 to 7 were changed, and the other operation steps and the test procedures were the same as those of example 1, and the specific setting manner is shown in table 5.

TABLE 5

Shampoo foam height test

(1) Weighing anhydrous magnesium sulfate (MgSO)₄)3.7g and anhydrous calcium chloride (CaCl)₂)5.0g, dissolved sufficiently in 5000mL of distilled water to give hard water having a hardness of 1500 mg/kg.

(2) Preheating the super thermostat to 40 +/-1 ℃ and keeping the temperature of the Roche foam instrument at 40 +/-1 ℃. Weighing 2.5g of sample (shampoo prepared in example), adding 900mL of distilled water for dissolving, adding 100mL of 1500mg/kg hard water, heating to (40 +/-1) DEG C, and stirring uniformly to dissolve the sample to obtain a test solution.

(3) A portion of the sample was aspirated through a 200mL volumetric funnel and rinsed down the wall tube of the foam meter. And then placing the test solution into the bottom of a foam instrument to be aligned with the standard scale to 50mL, sucking the test solution by using a 200mL quantitative funnel, fixing the central position of the funnel, immediately recording the foam height after the test solution is placed, taking the average value of results with the errors in the two times within an allowable range as a final result, and keeping the result to an integer number. At the end of 5 minutes, the foam height is again noted, and the result averaged over two times with an error in the allowed range is taken as the final result, with the result being retained to integer numbers. The results are shown in Table 6.

TABLE 6

	0min foam height (mm)	5min foam height (mm)
			Example 1	85	75
Example 2	110	103
			Example 3	120	115
Example 4	80	71
			Example 5	75	65

As can be seen from the data in table 6, 4 foaming surfactants (including decyl glucoside, disodium cocoyl glutamate, sodium methylcocoyl taurate, and cocamidopropyl betaine) were added in example 3, and the amount of surfactant added was large, the most foam was generated, and the foam-sustaining power was strong.

In example 1, the types of the primary surfactant and the secondary surfactant are unchanged, but the addition amount is the lowest value, and the foam richness and foam endurance are close to those of example 4.

The types of the primary surfactant and the auxiliary surfactant in example 2 were unchanged, but the addition amounts were not high enough, and the degree of foam richness and foam durability were lower than those in example 3.

In example 4, the absence of one of the primary surfactants, decyl glucoside, produced insufficient lather and insufficient lather duration.

Example 5 with the addition of two surfactants (including decyl glucoside and cocamidopropyl betaine), the foam was not abundant enough and foam persistency was not strong.

Scalp use test

45 female volunteers with age range of 18-60 years are selected and randomly divided into 9 groups of 5 persons each. The first 8 groups were experimental groups, and the shampoos prepared in examples 1 to 5 and comparative examples 5 to 7 were used, respectively; the last group is blank group, compared with the shampoo in the embodiment 3, the used shampoo is not added with the lucid ganoderma fermentation liquid, and other raw materials and the preparation method are the same as the embodiment 3. The shampoo is used every day or every other day according to the habit of a user, and the experimental time is 28 days.

The shampoo using method comprises the following steps: fully moistening the whole scalp, rubbing 5-10 mL of shampoo to remove foams, and then applying the foams on scalp hairlines. The finger abdomen is kneaded for 3-5 minutes and then cleaned with clear water.

Selecting the center of the vertex as a test part, testing the moisture content of the stratum corneum of the scalp by using a moisture tester probe Corneometer CM825 of a skin moisture tester at 0, 15 and 30 days of the experiment, and averaging the test three times, wherein the result is shown in Table 7; scalp percutaneous water loss was measured on day 0, 15, 30 of the experiment using a very small skin water loss TEWL test probe, Tewameter (TM) Nano, and the results are averaged over three tests and are shown in Table 8.

As can be seen from tables 7 and 8, the water content of the scalp stratum corneum of the volunteers using examples 1 to 5 (shampoo containing ganoderma lucidum fermentation liquid) was increased and the transdermal water loss of the scalp was decreased at day 28, and compared with the volunteers using comparative example 5 (ganoderma lucidum extract), the water content of the scalp stratum corneum was increased, the transdermal water loss of the scalp was decreased more significantly, and the blank group was not significantly changed. Comparative examples 6 and 7 compared with example 3, the moisture content of the scalp cuticle was increased, and the reduction of the scalp percutaneous water loss was not so significant, probably because the ratio of the added sporocarp to the spore powder in the preparation of the added ganoderma lucidum fermentation liquid was different, and the ratio of the added sporocarp to the spore powder in example 3 was 1:1, the ratio of the added ganoderma lucidum fermentation liquor in the comparative examples 6 and 7 is 1: 11 and 11: 1, the range is out, which shows that the efficacy of adding the ganoderma lucidum fermentation liquor with the fermentation ratio of (10-1): 1-10 on moisture retention and scalp percutaneous water loss is better.

As shown above, the shampoo containing the lucid ganoderma fermentation liquid is added into the shampoo, so that the scalp moistening effect is achieved, the water content of the horny layer of the scalp can be increased, and the dandruff reducing effect is facilitated. Scalp transdermal water loss is associated with the scalp barrier, and reduced scalp transdermal water loss means enhanced scalp barrier function and also reduced dandruff production.

TABLE 7

TABLE 8

Dandruff control test for shampoo

The tester: 20 volunteers with significant dandruff were recruited as subjects, and the head of all the subjects showed an excess of complex dandruff such as flaky white scales. All the hair-washing, hair-protecting and daily-use chemical products with the anti-dandruff effect were stopped for the first two weeks of the test and randomly divided into 2 groups of 10 people.

The using method comprises the following steps: one group of subjects used the shampoo containing the ganoderma lucidum fermentation liquid prepared in example 3, and the other group was blank group, and the shampoo used was not added with the ganoderma lucidum fermentation liquid compared with example 3, and other raw materials and preparation methods were the same as example 3. A qualified dermatologist was fixed for evaluation.

The clinical evaluation method comprises the following steps: clinical evaluation was performed using ASFS score (adhesive scalp scraping score) with no visible desquamation of 0 score; the occasional tiny desquamation of hair roots is 1 minute; a small amount of large desquamation or a large amount of fine desquamation is 3 minutes; the large amount of desquamation is 4 minutes. The scoring was done before shampooing, on days 0, 14, and 28 at the start of the experiment.

The results are shown in table 9, and it can be seen that the average ASEF score of the subjects using the shampoo containing the ganoderma lucidum fermentation liquid is reduced from 2.79 to 2.01 from day 0 to day 28, and the average ASEF score is reduced more than that of the blank group, probably because the example 3 combines the ganoderma lucidum fermentation liquid and the piroctone olamine salt, the effect is better than that of the blank group, and the shampoo containing the ganoderma lucidum sporophore spore powder fermentation liquid can improve the dandruff better.

TABLE 9

ASFS score	Day 0	14 days	28 days
				Example 3	2.79	2.57	2.01
Blank group	2.83	2.66	2.32

The above description is only for the specific embodiments of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art can easily conceive of the changes or substitutions within the technical scope of the present invention, and all the changes or substitutions should be covered within the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.

Claims

1. The shampoo containing the ganoderma lucidum sporophore spore powder fermentation liquid is characterized by comprising the following raw materials in parts by weight based on 100 parts by weight: 10-15 parts of decyl glucoside, 10-15 parts of disodium cocoyl glutamate, 3-8 parts of sodium methyl cocoyl taurate, 3-8 parts of cocamidopropyl betaine, 0.2-0.6 part of acrylic acid (ester)/C10-30 alkanol acrylate cross-linked polymer, 1-2 parts of propylene glycol laurate, 0.5-1 part of PPG-3 octyl ether, 5-10 parts of ganoderma lucidum sporophore spore powder fermentation liquid, 0.1-0.5 part of dipotassium glycyrrhizinate, 0.1-1 part of aminomethyl propanol, 0.2-0.5 part of piroctone ethanolamine salt, 1-2 parts of PEG-7 glyceryl cocoate, 0.05-0.2 part of argan oil, 0.4-0.8 part of phenoxyethanol, 0.5-1 part of ethylhexyl glycerol, 0.01-0.1 part of citric acid, 0.05-0.1 part of disodium EDTA and 32-66 parts of pure water;

(3) mixing and fermenting: carrying out amplification culture on the activated strains, mixing to obtain a mixed seed solution, adding the mixed seed solution into the sterilized ganoderma lucidum fermentation substrate, and fermenting to obtain initial ganoderma lucidum sporophore spore powder fermentation liquor;

(4) and (3) fermentation post-treatment: sterilizing the initial ganoderma lucidum spore powder fermentation liquor, crushing thalli, and filtering to obtain the ganoderma lucidum spore powder fermentation liquor;

in the step (3), the mass ratio of the bifidobacterium adolescentis CICC 6175, the bacillus natto CICC 10262, the lactobacillus bulgaricus CICC 20271 and the saccharomyces cerevisiae CICC 1252 in the mixed seed liquid is (1.5-2.5) to (1.5) to (2.5): (0.8-1.2).

2. The shampoo containing the fermentation solution of spore powder of ganoderma lucidum fruiting body according to claim 1, wherein the shampoo further comprises 0.3-0.8 parts of essence.

3. The method for preparing the shampoo containing the ganoderma lucidum spore powder fermentation liquid according to claim 2, which is characterized by comprising the following steps:

uniformly mixing 28-60 parts of pure water, disodium EDTA, decyl glucoside, disodium cocoyl glutamate, sodium methyl cocoyl taurate, cocamidopropyl betaine, propylene glycol laurate and PPG-3 octyl ether to obtain a phase B for later use;

uniformly mixing the PEG-7 glyceryl cocoate, the ganoderma lucidum sporophore spore powder fermentation liquor, the argan oil and the essence to obtain a C phase for later use;

uniformly mixing the piroctone olamine salt and dipotassium glycyrrhizinate to obtain a phase D for later use;

uniformly mixing the phenoxyethanol and the ethylhexyl glycerin to obtain an E phase for later use;

uniformly mixing the aminomethyl propanol and the citric acid to obtain an F phase for later use;

(3) adding the phase B into a stirring pot, preserving heat and stirring for 20-30min, and then cooling;

(6) adding the phase E and the phase F into the stirring pot, uniformly stirring, adjusting the pH value to 6-6.5, and discharging.

4. The shampoo containing the ganoderma lucidum spore powder fermentation liquid according to claim 1, wherein in the step (1), the mass ratio of the ganoderma lucidum spore powder to the ganoderma lucidum spore powder is (10-1): (1-10).

5. The shampoo containing the ganoderma lucidum sporophore and spore powder fermentation liquid according to claim 4, wherein in the step (1), the mass ratio of the ganoderma lucidum sporophore to the spore powder is 1: 1.

6. the shampoo containing the spore powder fermentation liquid of the ganoderma lucidum fruiting body according to claim 1, wherein in the step (3), the mass ratio of bifidobacterium adolescentis CICC 6175, bacillus natto CICC 10262, lactobacillus bulgaricus CICC 20271 and saccharomyces cerevisiae CICC 1252 in the mixed seed liquid is 2:1:2: 1.

7. The shampoo containing the ganoderma lucidum spore powder fermentation liquid according to claim 1, wherein in the step (3), the mixed seed liquid is added into the sterilized ganoderma lucidum fermentation substrate in a mass fraction of 1.5-2.5%.

8. The shampoo containing the spore powder fermentation liquid of Ganoderma lucidum fruiting body as claimed in claim 7, wherein in step (3), the fermentation temperature is 35-45 deg.C, and the fermentation is carried out for 44-52h on a shaker with a rotation speed of 100-.

9. The shampoo containing the spore powder fermentation liquid of the ganoderma lucidum fruiting body according to claim 1, wherein in the steps (1) and (4), the sterilization treatment is sterilization at 121 ℃ for 15min or ultra-high pressure sterilization at 600MPa and 25 ℃.

10. The shampoo containing the spore powder fermentation liquid of the ganoderma lucidum fruiting body according to claim 1, wherein in the step (4), the thallus is crushed by a high pressure homogenizer or ultrasonic crushing, and the filtration is performed by centrifugation, an ultrafiltration membrane, a microfiltration membrane or reverse osmosis filtration.