CN115125108A - Prevent polluting cell cultivation equipment - Google Patents

Abstract

The invention provides a cell cultivation device capable of preventing pollution, which comprises a main body, an air supply assembly and a cultivation assembly, wherein a hollow cavity with a top part penetrating through is arranged in the main body, the bottom of the main body is connected with a gas pipe which extends to the inner part of the hollow cavity in a penetrating way, the inner wall of the inner side of the main body is provided with a heat preservation layer, and the inner wall of the main body at the inner side of the heat preservation layer is embedded with a heating wire, the inner wall of the main body at the top of the gas pipe is provided with an installation groove, the cultivating component is movably connected in the mounting groove, the inner wall of the top of the main body is fixedly connected with the screw wall, and the air supply component is screwed on the top of the main body through the screw wall, the invention provides a device for preventing cell cultivation from being polluted, which aims to solve the problems that the prior cell cultivating device has a complex structure, the inside is not convenient and fast to clean after the cell cultivation, and the cleaning dead angle is easy to exist to influence the cell cultivation effect, and the problem that bacteria in the air can easily enter the interior of the existing open cell culture device after the interior of the cell culture device is disinfected, so that a sterile environment can not be formed.

Description

Prevent polluting cell cultivation equipment

Technical Field

The invention relates to the field of cell cultivation, in particular to a cell cultivation device capable of preventing pollution.

Background

The cell culture mainly refers to a method for simulating in-vivo environment (sterility, proper temperature, pH value, certain nutritional conditions and the like) in vitro to enable the in-vivo environment to survive, grow and reproduce and maintain main structure and functions, and is mainly used in the fields of new drug screening, vaccine research and development, gene engineering drug research and development, cell engineering drug research and development and the like.

However, the environment required during cell cultivation is very harsh, firstly to maintain its sterility and proper temperature, and secondly to maintain the nutrients and pH value required for cell division. The current cell culture device structure is complicated and it is very inconvenient that inside is clean after the cell is cultivated, and still exists the problem that clean dead angle influences the cell and cultivates the effect easily to and the bacterium in the inside disinfection back air of current open cell culture device gets into its inside unable sterile environment's that forms problem easily.

Disclosure of Invention

In order to solve the technical problems, the invention provides a cell culture device capable of preventing pollution, which aims to solve the problems that the existing cell culture device is complex in structure, the inside of the cell culture device is not convenient and fast to clean after the cell culture, cleaning dead angles exist easily and the cell culture effect is influenced, and bacteria in the air easily enter the inside of the existing open cell culture device after the inside of the existing open cell culture device is disinfected, so that a sterile environment cannot be formed.

The invention relates to a purpose and an effect of preventing cell cultivation equipment from being polluted, which are achieved by the following specific technical means: the utility model provides a prevent polluting cell cultivation equipment, includes main part, air feed subassembly, cultivates the subassembly, the main part is inside to be seted up the cavity that the top runs through, and main part bottom through connection has the gas-supply pipe that extends to the cavity inside, be equipped with the heat preservation in the inboard inner wall of main part, and the inboard main part inner wall embedding of heat preservation has the heater strip, seted up the mounting groove on the main part inner wall at gas-supply pipe top, and cultivate subassembly swing joint in the mounting groove, fixedly connected with spiral shell wall on the inner wall of main part top, and the air feed subassembly passes through spiral shell wall spiro union in main part top.

Wherein, the whole setting that is "T" shape cylinder that is of air feed subassembly, and the subassembly of just ventilating has seted up the air guide groove inside, air guide groove bottom fixedly connected with air sterilizing equipment, and air sterilizing equipment both ends port all is equipped with the filter screen. And meanwhile, the wind power driving device is fixedly connected inside the transverse end of the air guide groove, the nutrient box is embedded into the two sides inside the air supply assembly, and the spiral grooves matched with the spiral walls are formed in the inner walls of the air assemblies on the two sides of the nutrient box.

The utility model discloses a nutrition box, including nutrition box, movable groove, piston board, transmission line, pneumatic drive device, transmission line, wherein, nutrition box top through connection has the filling mouth that extends to the outer gas supply subassembly top, and nutrition box bottom is equipped with the shower nozzle that extends to the outer gas supply subassembly bottom, set up movable groove in the inside upper inner wall of nutrition box, and swing joint has the one-way piston board that switches on in the movable groove, fixedly connected with return spring between piston board bottom and the movable groove bottom simultaneously, through connection has the transfer line with the meshing of wind-force drive device on the nutrition box inner wall at movable groove top, and the top fixedly connected with that the transfer line is located the inside of nutrition box and the eccentric wheel of piston board meshing.

Wherein, cultivate fixedly connected with handle on the subassembly outside outer wall, and cultivate the through-hole that runs through from top to bottom set up at the subassembly center, fixedly connected with gas transmission ring in the through-hole simultaneously, gas transmission ring top turn-on connection has the telescopic air duct, and opens on the air duct outside pipe wall and evenly set up the bleeder vent, and air duct top turn-on connection has the cultivation dish the same with main part inside diameter simultaneously.

Wherein, cultivate and seted up the annular in the dish lateral wall, and swivelling joint has clean ring in the annular, the pneumatic means that fixedly connected with switched on with the air duct in the annular of clean ring bottom portion simultaneously, cultivate the dish center and set up the through-hole that switches on from top to bottom, and swivelling joint has the returning face plate in the through-hole, swing joint has the steering column that runs through from top to bottom in the cultivation dish inner wall between annular and the through-hole, and is equipped with the screw thread on the steering column shaft, steering column and returning face plate and pneumatic means meshing simultaneously.

Wherein, pneumatic means includes the output tube, the output tube be located the ring channel bottom and with air duct conductive connection, and the venthole has been seted up at the air duct top, the venthole center is rotated and is connected with the guide board, and the inboard fixedly connected with pivot of guide board, the epaxial fixedly connected with of pivot simultaneously with the steering column meshing turn to the connecting rod to turn to the end pole body of connecting rod is equipped with the screw thread.

Wherein, swivelling joint has the clean subassembly of independent setting on the clean ring body, and clean subassembly is including clean movable groove, the air guide groove of subtend setting is seted up to clean movable groove both sides, and the inside swing joint of clean movable groove has and extends to clean soft board, and the clean soft board of the inside clean movable groove of while is on one's body fixedly connected with clean cotton, the thread groove has been seted up to the clean soft board of air guide inslot portion, and the inside center of air guide groove rotates and is connected with the screw rod, and the meshing of screw rod top is connected with the drive block simultaneously to fixedly connected with spring between drive block and the clean subassembly.

Wherein, the connecting screw rod of returning face plate both ends fixedly connected with and steering column meshing, and the returning face plate bottom has been seted up and has been cultivated a groove, has seted up the spiral shell groove in the returning face plate inner wall of cultivating a groove top simultaneously, it has the cultivation cup to cultivate threaded connection in the groove of cup, and has seted up the air guide groove between the returning face plate of cultivating between the cup groove.

The cultivation cup comprises a cultivation cup body, a cultivation cup top end and a cultivation cup bottom end, wherein the cultivation cup top end is fixedly connected with a screw connection block, power grooves are formed in inner walls of two sides of the cultivation cup body, meanwhile, driving screws are connected in the power grooves in a rotating mode, door shafts meshed with the driving screws are connected in the inner walls of cup openings of the cultivation cup body in a rotating mode, and door plates are fixedly connected to the inner sides of the door shafts.

The clamping groove is formed in the inner walls of the two sides of the power groove, the power groove is movably connected with the screw connection block, a screw hole meshed with the driving screw rod is formed in the center of the screw connection block, and the clamping blocks are limited in the clamping groove in the two sides of the screw connection block.

Has the advantages that:

(1) through being equipped with the air feed subassembly, the cultivation dish in the subassembly is cultivated in the utilization makes the inside air guide groove that passes through in the air feed subassembly of main part form the air current in the inside air guide groove that removes compression centrum in the main part, and the air current of inside and outside will drive the wind-force drive arrangement in the air guide groove and for the inside power that provides of nutrition box this moment to inside and outside air will disappear through the bacterium in air sterilizing equipment to the air and kill, realizes disinfecting the inside air of main part and for the inside effect that provides power of nutrition box.

(2) Through being equipped with the nutrition box, the piston plate that utilizes the inside one-way conduction of nutrition box can be inside leading-in box of nutrient solution to will drive the transfer line rotation when wind power drive arrangement functions, the eccentric wheel of transfer line end will cooperate return spring to make the drive piston plate reciprocate in the activity groove this moment, utilizes its one-way conduction setting to pressurize the nutrition box is inside, realizes that the nutrient solution passes through the shower nozzle with the inside effect that provides the nutrient for the cell cultivation of nutrient solution leading-in post main part.

(3) Through being equipped with and cultivating the subassembly, when utilizing the gas-supply pipe to the inside output steam of main part, steam will get into the inside top of gas-guide pipe through the gas-supply ring and move cultivation dish and remove to the main part top, gas pocket on the gas-guide pipe wall at this moment exports steam and carries out pasteurization to the inside inner wall of main part, it also will clean the inside inner wall of main part to cultivate the clean ring in the dish outer wall simultaneously under the blowing of steam, treat to cultivate to make cultivation dish retrieve the cultivation dish to cultivating the subassembly inside through the gas-supply pipe suction with the inside steam of main part after the dish removes the main part top, the realization utilizes steam cooperation to cultivate the dish and cleans the effect of disinfecting inside the main part.

(4) Through being equipped with and cultivate the dish, utilize and cultivate the dish when removing the main part top under steam drives, cultivate the steering column in the dish this moment and will drive the returning face plate upset and drive the change of pneumatic means air current direction, realize that the inside clean back that finishes of main part will cultivate the inside effect of cup upset subtend main part.

Drawings

FIG. 1 is a schematic view of the overall structure of the present invention.

FIG. 2 is a schematic view of the overall disassembled structure of the present invention.

FIG. 3 is a schematic top view of the main body of the present invention.

FIG. 4 is a schematic side sectional view of the gas supply assembly of the present invention.

FIG. 5 is a schematic diagram of the side view cross-sectional structure of the nutrition box of the present invention.

Fig. 6 is a schematic structural view of a cultivation assembly of the present invention.

Fig. 7 is a partial structure view of the cultivation assembly of the present invention.

FIG. 8 is a schematic view of the structure of the cultivation plate of the present invention.

FIG. 9 is a schematic side sectional view of the cultivation plate of the present invention.

FIG. 10 is an enlarged view of the structure at A in FIG. 9 according to the present invention.

FIG. 11 is a schematic top view of a cleaning ring according to the present invention.

FIG. 12 is an enlarged view of the structure at B in FIG. 11 according to the present invention.

FIG. 13 is a side sectional view of the roll-over panel of the present invention.

FIG. 14 is a schematic side sectional view of the cultivation cup of the present invention.

FIG. 15 is an enlarged view of the structure of FIG. 14 at C according to the present invention.

In fig. 1 to 15, the correspondence between the component names and the reference numbers is:

1-main body, 101-air pipe, 102-spiral wall, 103-mounting groove, 104-insulating layer, 105-heating wire, 2-air supply component, 201-air guide groove, 202-spiral groove, 203-air sterilization device, 204-wind power driving device, 205-nutrition box, 206-movable groove, 207-return spring, 208-transmission rod, 209-eccentric wheel, 210-spray head, 211-piston plate, 3-cultivation component, 301-cultivation disc, 302-air transmission ring, 303-air guide pipe, 304-cleaning ring, 305-annular groove, 306-steering column, 307-turnover plate, 308-connecting stud, 309-output pipe, 310-air outlet hole, 311-guide plate, 312-steering connecting rod, 313-cleaning component, 314-a cleaning movable groove, 315-an air guide groove, 316-a cleaning soft board, 317-cleaning cotton, 318-a driving block, 319-a screw rod, 320-a cultivating cup groove, 321-a screw groove, 322-a cultivating cup, 323-an air guide groove, 324-a power groove, 325-a driving screw rod, 326-a door shaft, 327-a door plate, 328-a clamping groove, 329-a screw connecting block, 330-a clamping block and 331-a pneumatic device.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, rather than all embodiments, and all other embodiments obtained by a person of ordinary skill in the art without creative efforts based on the embodiments of the present invention belong to the protection scope of the present invention.

The first embodiment is as follows:

as shown in figures 1 to 5:

firstly, the cells to be cultivated are filled into the cultivating component 3, the cultivating component 3 is fixed inside the main body 1 through the mounting groove 103, steam is filled into the main body 1 through the air conveying pipe 101 at the moment, the cultivating tray 301 in the cultivating component 3 moves towards the top of the main body 1 under the action of the steam and conducts sterilization and disinfection operation on the inside of the main body 1, the air inside the main body 1 is discharged through the air supply component 2, the steam is extracted out through the air conveying pipe 101 after the cultivating tray 301 moves to the top of the main body 1, at the moment, the cultivating tray 301 moves towards the bottom of the main body 1, and meanwhile, the external air is guided into the main body 1 through the air supply component 2.

When the air supply assembly 2 supplies air to the inside of the main body 1, the air passes through the air guide groove 201 and drives the wind power driving device 204 to rotate, and is purified through the air sterilization device 203 so as to ensure the sterile environment inside the main body 1, when the wind power driving device 204 rotates under the driving of air flow, the driving rod 208 is driven to rotate along with the air flow, at the moment, the eccentric wheel 209 at the tail end of the driving rod 208 drives the piston plate 211 to move up and down in the movable groove 206 on the inner wall of the nutrient box 205 in a matching way with the return spring 207, the arrangement of unidirectional conduction of the piston plate 211 is utilized so as to increase the pressure inside the nutrient box 205, the effect of supplying nutrients to the cell culture by spraying the nutrient solution inside the nutrient box 205 on the culture assembly 3 through the spray head 210 is realized, and the temperature suitable for cell culture is kept inside the main body 1 in a matching way with the heat preservation layer 104 and the heating wire 105.

The second embodiment:

as shown in fig. 6 to 10:

when steam is input from the bottom of the main body 1, the steam enters the air duct 303 in the cultivating component 3 through the air duct ring 302, at the moment, the air duct 303 pushes the cultivating tray 301 to the top of the main body 1 due to the steam entering expansion, and the air holes in the wall of the air duct 303 guide the steam to the inner wall of the main body 1 to kill bacteria on the inner wall at high temperature, and meanwhile, the cleaning ring 304 in the side wall of the cultivating tray 301 is driven by the starting device 331 to rotate to clean the inner wall of the main body 1.

Steam enters the inner part of the output pipe 309 communicated with the air guide pipe 303 while steam is input into the air guide pipe 303, at the moment, the steam is blown to the inner side of the cleaning ring 304 under the guidance of the guide plate 311, at the moment, the cleaning ring 304 rotates anticlockwise to clean the inner wall of the main body 1 and provide power for the cultivation plate 301 to move towards the top of the main body 1.

Example three:

as shown in figures 5 to 10:

when the cultivating tray 301 moves to the top in the main body 1, the steering column 306 on the tray body will contact with the bottom of the air supply assembly 2 and move to the bottom of the cultivating tray 301, at this time, the steering column 306 drives the connecting screw 308 to rotate by using the screw thread on the column body so as to turn the turning plate 307 by 180 degrees, and simultaneously drives the steering link 312 to move to the inside/outside of the output pipe 309, so that the guide plate 311 changes the angle so as to blow the steam to the outside of the cleaning ring 304, thereby realizing the rotation of the cleaning ring 304 and providing the cultivating tray 301 with the power to move to the bottom of the main body 1.

Example four:

as shown in fig. 11 to 12:

when steam is guided to the cleaning ring 304, the steam enters the air guide groove 315 and pushes the driving block 318 to move in the air guide groove 315, at this time, the driving block 318 drives the screw 319 positioned in the air guide groove 315 to rotate by using the screw groove at the center thereof, and the cleaning soft plate 316 engaged with the screw 319 moves downward to the outside of the cleaning movable groove 314 by the screw thread of the screw 319 so that the cleaning cotton 317 protrudes out of the cleaning movable groove 314 to clean the inner wall of the main body 1.

Example five:

as shown in fig. 12 to 15:

when the turning plate 307 is driven by the steering column 306 to rotate 180 degrees by the connecting stud 308, the mouth of the cultivation cup 322 in the cultivation cup groove 320 faces the top of the main body 1, at this time, the screw block 329 in the power groove 324 of the cup wall of the cultivation cup 322 drives the driving screw 325 to rotate by the screw groove at the center under gravity, and the door shaft 326 engaged with the driving screw 325 rotates to open the door plate 327, so that nutrient solution can enter the inside of the cultivation cup 322 to provide nutrients for cell cultivation, and the effect of preventing the cell from being polluted by opening and closing the cultivation cup 322 after the inside of the main body 1 is cleaned is realized.

The working principle is as follows: firstly, cells to be cultivated are loaded into the cultivating component 3, the cultivating component 3 is fixed in the main body 1 through the mounting groove 103, steam is charged into the main body 1 through the air pipe 101, the cultivating tray 301 moves to the top of the main body 1 under the action of the steam and carries out sterilization and disinfection operation on the interior of the main body 1, when the cultivating tray 301 moves to the top of the main body 1, the steering column 306 on the tray body contacts with the bottom of the air supply component 2 and moves to the bottom of the cultivating tray 301, the steering column 306 drives the connecting screw 308 to rotate by using the screw thread on the column body so as to turn over the turnover plate 307 by 180 degrees, the screw block 329 in the cup wall power groove 324 of the cultivating cup 322 drives the driving screw 325 to rotate by using the screw groove at the center under gravity, simultaneously, the door shaft 326 meshed with the driving screw 325 rotates to open the door plate 327, so that nutrient solution can enter the cultivating cup 322, provide nutrients for cell culture and realize the effect of preventing the cells from being polluted by opening and closing the culture cup 322 after the inside of the main body 1 is cleaned.

And when air supply subassembly 2 provided the air to main part 1 inside, the air will be through leading air duct 201 and drive wind-force drive arrangement 204 rotation for the inside piston plate 211 of nutrition box 205 provides the power of operation, increases the inside pressure of nutrition box 205, sprays the nutrient solution through shower nozzle 210 on cultivating the subassembly 3 and cultivate the effect of providing the nutrient for cell cultivation, and cooperation heat preservation 104 and heater strip 105 make main part 1 inside keep the temperature that is fit for cell cultivation.

Claims (10)

1. The utility model provides a prevent polluting cell cultivation equipment, includes main part (1), air feed subassembly (2), cultivates subassembly (3), main part (1) inside sets up the cavity that the top runs through, and is equipped with spiral shell wall (102) that are used for fixed air feed subassembly (2) on main part (1) top inner wall, main part (1) inboard inner wall is equipped with heat preservation (104), and is equipped with heater strip (105) in heat preservation (104) inboard main part (1) inner wall, and main part (1) bottom is equipped with simultaneously runs through to main part (1) inside gas-supply pipe (101), its characterized in that: main part (1) top is equipped with air feed subassembly (2) that are used for purifying main part (1) inside air, and main part (1) bottom inner wall has seted up mounting groove (103), cultivates subassembly (3) swing joint simultaneously in mounting groove (103).

2. A contamination cell cultivating apparatus according to claim 1, wherein: air feed subassembly (2) are "T" shape setting, and air feed subassembly (2) inside air guide groove (201) of having seted up, bottom fixedly connected with air sterilizing equipment (203) in air guide groove (201) simultaneously to air guide groove (201) both ends are equipped with wind-force drive arrangement (204), the inside both sides of air feed subassembly (2) are equipped with nutrition box (205) with wind-force drive arrangement (204) meshing.

3. A contamination cell cultivating apparatus according to claim 2, wherein: the inside pressure boost part that is equipped with of nutrition box (205), and nutrition box (205) top is equipped with the filler, and nutrition box (205) bottom is equipped with nozzle (210) that extend to outside air feed subassembly (2) bottom simultaneously.

4. A contamination cell cultivating apparatus according to claim 1, wherein: the through-hole that runs through from top to bottom is seted up at cultivation subassembly (3) center, and fixedly connected with gas transmission ring (302) in the through-hole, gas transmission ring (302) top turn-on connection has telescopic air guide, and air guide top turn-on connection has cultivation dish (301).

5. A contamination cell cultivating apparatus according to claim 4, wherein: be equipped with clean ring (304) in breeding dish (301) lateral wall, and clean ring (304) bottom is equipped with pneumatic means (331), and breeding dish (301) center swivelling joint has returning face plate (307) simultaneously to be equipped with between clean ring (304) and returning face plate (307) steering column (306).

6. A contamination cell cultivating apparatus according to claim 5, wherein: the pneumatic device (331) comprises an output pipe (309), the bottom of the output pipe (309) is connected with the air guide pipe (303) in a conduction mode, and the top of the output pipe (309) is provided with a guide part capable of adjusting the air outlet.

7. A contamination cell cultivating apparatus according to claim 5, wherein: the cleaning ring (304) is provided with a rotatable cleaning component (313) on the ring body, the cleaning component (313) comprises a cleaning movable groove (314), air guide grooves (315) are formed in the ring body of the cleaning ring (304) on two sides of the cleaning movable groove (314), movable cleaning components are arranged in the cleaning movable groove (314), and meanwhile, a transmission component for driving the cleaning components to move is arranged in the air guide grooves (315).

8. A cell culturing preventing apparatus as claimed in claim 5, wherein: the bottom of the turnover plate (307) is provided with a cultivating cup groove (320), a cultivating cup (322) is arranged in the cultivating cup groove (320), and the plate body between the turnover plates (307) between the cultivating cup grooves (320) is provided with an air guide groove (323).

9. A contamination cell cultivating apparatus according to claim 8, wherein: the bottom of the cultivating cup (322) is provided with a screw joint block (329) connected with the cultivating cup groove (320), the inner wall of the cup opening of the cultivating cup (322) is provided with a door plate (327) which can be opened and closed by gravity, and the inner walls of two sides of the cultivating cup (322) are provided with power grooves (324).

10. A contamination cell cultivating apparatus according to claim 9, wherein: clamping grooves (328) are formed in the inner walls of the two sides of the power groove (324), a driving screw rod (325) is arranged in the power groove (324), and meanwhile, a threaded connection block (329) meshed with the driving screw rod (325) is arranged in each clamping groove (328).

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