CN202246691U - Ultrasonic atomizing type aseptic inoculation box - Google Patents
Ultrasonic atomizing type aseptic inoculation box Download PDFInfo
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- CN202246691U CN202246691U CN 201120398224 CN201120398224U CN202246691U CN 202246691 U CN202246691 U CN 202246691U CN 201120398224 CN201120398224 CN 201120398224 CN 201120398224 U CN201120398224 U CN 201120398224U CN 202246691 U CN202246691 U CN 202246691U
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- atomizing
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/34—Internal compartments or partitions
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- Apparatus For Disinfection Or Sterilisation (AREA)
Abstract
The utility model relates to an ultrasonic atomizing type aseptic inoculation box which consists of an ultrasonic atomizer, an inoculation box, a feeding/discharging box and an atomizing chamber, wherein the ultrasonic atomizer is communicated with the atomizing chamber; the inoculation box and the feeding/discharging box are arranged on the atomizing chamber in parallel; micro-pores which are communicated with the inoculation box and the feeding/discharging box are distributed at the top of the atomizing chamber; the inoculation box and the feeding/discharging box are separated by a foldable door; ultraviolet lamps are respectively mounted on the top of the inoculation box and the feeding/discharging box; the feeding/discharging box is provided with a side door; the inoculation box is provided with a circular hole; a connecting arm glove is arranged in the circular hole; a power control device and a circuit adapter are arranged on the top of the ultrasonic atomizer; the atomizing chamber is arranged at the bottom of the ultrasonic atomizer; and an ultrasonic energy exchanger is mounted in the atomizing chamber. The ultrasonic atomizing type aseptic inoculation box is big in atomizing and disinfecting contact surface area and is free from dead angle. Tracking atomizing and disinfecting operations are performed during the whole process, so that an aseptic condition of a working area is extremely ensured all the time and the microbial problem caused by careless operation is solved.
Description
Technical field
The utility model relates to and be applied to bacterium, the inoculating facility of bacterial classifications such as edible mushrooms, what be specifically related to is a kind of ultrasonic atomization formula aseptic inoculation case.
Background technology
The culture of mikrobe or contain the operating process that the sample of mikrobe is transplanted on the substratum and inoculate exactly.The cultivation inoculation of mikrobe is the important technology of bioengineering field, is a operative technique the most basic in the scientific research.The morphologic observation of the separation of mikrobe, cultivation, purifying or evaluation and related microorganism and physiological Study all must be inoculated.The key of inoculation is the aseptic technique of carrying out of wanting strict, and causes pollution like careless manipulation, then influences the carrying out of whole work, and existing inoculation tank has and adopts disinfection by ultraviolet light, but sterilisation effect is not very desirable.
Summary of the invention
The purpose of the utility model provides a kind of ultrasonic atomization formula aseptic inoculation case, and this ultrasonic atomization formula aseptic inoculation case is used to solve the unfavorable problem of inoculation tank sterilisation effect of present use.
The utility model solves the technical scheme that its technical problem adopted: this ultrasonic atomization formula aseptic inoculation case is made up of ultrasonic atomizer, inoculation tank, turnover hopper, aerosol chamber; Ultrasonic atomizer is communicated with the aerosol chamber; Inoculation tank, turnover hopper are arranged side by side on the aerosol chamber, and the top of aerosol chamber is covered with the micropore that communicates with inoculation tank, turnover hopper; Separate through accordion door between inoculation tank and the turnover hopper, inoculation tank is respectively installed a uv lamp with the top of turnover hopper, and the turnover hopper is provided with side door; Inoculation tank is provided with circular hole, and the link arm gloves are installed in the circular hole; The top of ultrasonic atomizer is power control and circuit adapter, and the bottom of ultrasonic atomizer is an atomization cavity, and ultrasonic transducer is installed in the atomization cavity.
Direct current waterproof small fan is housed in the aerosol chamber in the such scheme.
Two water level control contact pilotages are installed on the ultrasonic transducer in the such scheme; Because being immersed in the shallow water aqueous solution, water level control contact pilotage works; If long-term atomizing; When in case liquid level reduces water level control contact pilotage being surfaced, ultrasonic transducer can quit work automatically, and this has also been avoided ultrasonic transducer to damage because of heating.
Inoculation tank is provided with storing platform in the such scheme, and storing platform can be placed on inoculating tool, inoculation material on the storing platform with the corresponding setting of link arm gloves, inoculum size for a long time, inoculates operation with vacating space.
It is the straight lamp tube of 220V, wavelength 253.7nm, power 30W that such scheme medium ultraviolet lamp adopts voltage, and this uv lamp sterilization effect is best.
The utlity model has following beneficial effect:
The utility model is through utilizing uv lamp radiation disinfection by ultraviolet light and ultrasonic atomization disinfecting; Aseptic degree is high; The long-pending big no dead angle of atomization disinfection surface in contact; The tracking control of full process atomization disinfection can guarantee to greatest extent that the WS is sterile environment all the time, thereby avoids careless manipulation to cause dying the problem of assorted bacterium.
Description of drawings
Fig. 1 is the structural representation of the utility model.
1. thimerosal mouth 2. side doors 3. ultrasonic atomizeies 4. ultrasonic atomization appliance mains switches 5. inoculation tanks 6. pass in and out hoppers 7. accordion doors 8. aerosol chambers 9. micropores, 10. uv lamps, 11. circular holes, 12. fog discharge passages, 13. nitrogen air inlet ports, 14. vent valves, 15. storing platforms, 16. direct current small fans, 17. liquidometers.
Embodiment
Below in conjunction with accompanying drawing the utility model is done and to be further described:
As shown in Figure 1; This ultrasonic atomization formula aseptic inoculation case is made up of ultrasonic atomizer 3, inoculation tank 5, turnover hopper 6, aerosol chamber 8; Casing one end is a turnover hopper 6; Turnover hopper 6 has a side door 2, and the casing the other end is a ultrasonic atomizer 3, and ultrasonic atomizer 3 is by 4 its work of control of ultrasonic atomization appliance mains switch; Separate through accordion door 7 between inoculation tank 5 and the turnover hopper 6, can open and close accordion door 7 at box house during experiment; Inoculation tank 5 is respectively installed a uv lamp 10 with the top of turnover hopper 6, and inoculation tank 5 is the glass appearance surface layer with the front and the end face of turnover hopper 6, is transparent, and other parts are that lighttight material is processed; Between ultrasonic atomizer 3 and the inoculation tank 5 fog discharge passage 12 is set; Ultrasonic atomizer 3 is communicated with through fog discharge passage 12 with aerosol chamber 8; Inoculation tank 5, turnover hopper 6 are arranged side by side on aerosol chamber 8, and the top of aerosol chamber 8 is covered with the micropore 9 that communicates with inoculation tank 5, turnover hopper 6.
Leave two circular holes 11 on the tank wall in inoculation tank 5 fronts, respectively connect link arm gloves in the circular hole 11, glove port is connected through flange with circular hole 11 junctions, good seal, and the link arm gloves can be medical rubber gloves; The bacterial classification of inoculation need feed nitrogen when being strict anerobes; Nitrogengas cylinder links to each other with nitrogen air inlet port 13; And open vent valve 14, and drain the oxygen in the inoculation tank 5, make anaerobic environment; Inoculum size can be placed on inoculating tool, inoculation material on the storing platform 15 for a long time, can inoculate operation by vacating space.
The top of ultrasonic atomizer 3 is power supply control and circuit adapter; The bottom of ultrasonic atomizer 3 is atomization cavities; Atomization cavity mainly comprises the ultrasonic transducer in water level control and the biasing circuit; Ultrasonic transducer is called atomising head again, two water level control contact pilotages installing on the ultrasonic transducer; Ultrasonic atomizer 3 is provided with defogging hole; Ultrasonic atomizer 3 and fog discharge passage 12 communicate through defogging hole, and ultrasonic atomizer 3 is delivered to aerosol chamber 8 to aerosol through fog discharge passage 12; Aerosol chamber's 8 planar dimensions are the size of inoculation tank 5 and turnover hopper 6 bottom surfaces; Direct current waterproof small fan 16 also is equipped with 8 inside in the aerosol chamber, and the top of aerosol chamber 8 has been covered with a lot of micropores 9, and aerosol gets into inoculation tank 5 through wind-force through micropore 9 and accomplishes disinfection with turnover hopper 6.
Ultrasonic transducer 3 shells are foundry goods of copper material, and copper shell chrome-faced polishing is packaged with transverter (nickel or titanium high frequency piezoelectric patches) in the copper shell.
In the utility model when sterilization, can and be connect the uv lamp 10 times that thing places inoculation tank 5 together bacterial classification, instrument, can not influence its surviving rate.Use certain density thimerosal to pour into atomization chamber through thimerosal mouth 1; From the amount that liquidometer 17 can be read thimerosal, the sterilization fog of generation carries out thorough disinfection through the top through micropore 9 entering inoculation tanks 5 to flowing down in direct current small fan Final 16 system through fog discharge passage 12 entering aerosol chambers 8 again.Open the sterilization that uv lamp 10 and ultrasonic atomization appliance mains switch 4 carried out 60 minutes simultaneously.Turn off uv lamp after the completion, suitably turn on the fog discharge passage 12 the defeated mist knob that is provided with down and again LS is opened, can carry out the inoculation of bacterial classification.
Claims (5)
1. ultrasonic atomization formula aseptic inoculation case; It is characterized in that: this ultrasonic atomization formula aseptic inoculation case is made up of ultrasonic atomizer (3), inoculation tank (5), turnover hopper (6), aerosol chamber (8); Ultrasonic atomizer (3) is communicated with aerosol chamber (8); Inoculation tank (5), turnover hopper (6) are arranged side by side on aerosol chamber (8), and the top of aerosol chamber (8) is covered with the micropore (9) that communicates with inoculation tank (5), turnover hopper (6); Separate through accordion door (7) between inoculation tank (5) and the turnover hopper (6), a uv lamp (10) is respectively installed at the top of inoculation tank (5) and turnover hopper (6), and turnover hopper (6) is provided with side door (2); Inoculation tank (5) is provided with circular hole (11), and circular hole is equipped with the link arm gloves in (11); The top of ultrasonic atomizer (3) is power control and circuit adapter, and the bottom of ultrasonic atomizer (3) is an atomization cavity, and ultrasonic transducer is installed in the atomization cavity.
2. ultrasonic atomization formula aseptic inoculation case according to claim 1 is characterized in that: direct current waterproof small fan is housed in described aerosol chamber (8).
3. ultrasonic atomization formula aseptic inoculation case according to claim 1 is characterized in that: two water level control contact pilotages are installed on the described ultrasonic transducer.
4. ultrasonic atomization formula aseptic inoculation case according to claim 1, it is characterized in that: described inoculation tank is provided with storing platform (15), storing platform (15) and the corresponding setting of link arm gloves.
5. ultrasonic atomization formula aseptic inoculation case according to claim 1 is characterized in that: it is the straight lamp tube of 220V, wavelength 253.7nm, power 30W that described uv lamp (10) adopts voltage.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201120398224 CN202246691U (en) | 2011-10-19 | 2011-10-19 | Ultrasonic atomizing type aseptic inoculation box |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201120398224 CN202246691U (en) | 2011-10-19 | 2011-10-19 | Ultrasonic atomizing type aseptic inoculation box |
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| Publication Number | Publication Date |
|---|---|
| CN202246691U true CN202246691U (en) | 2012-05-30 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201120398224 Expired - Fee Related CN202246691U (en) | 2011-10-19 | 2011-10-19 | Ultrasonic atomizing type aseptic inoculation box |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103571750A (en) * | 2013-07-10 | 2014-02-12 | 温州兆曜机械科技有限公司 | Method for improving inoculation process through aseptic operation box |
| CN104823552A (en) * | 2015-04-13 | 2015-08-12 | 王镱钧 | Seed treatment device and method based on ultraviolet light and ultrasonic mixed synchronization pulse |
| CN106282009A (en) * | 2016-11-10 | 2017-01-04 | 山东省科学院生态研究所 | A kind of solid materials fermenting device |
| CN106434303A (en) * | 2016-11-18 | 2017-02-22 | 天津开发区合普工贸有限公司 | Bacterial infection experiment device for cell culture dishes |
| CN106489850A (en) * | 2016-11-30 | 2017-03-15 | 四川省农业科学院蚕业研究所 | Cocoonery is used in a kind of Bombyx Batryticatus production |
-
2011
- 2011-10-19 CN CN 201120398224 patent/CN202246691U/en not_active Expired - Fee Related
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103571750A (en) * | 2013-07-10 | 2014-02-12 | 温州兆曜机械科技有限公司 | Method for improving inoculation process through aseptic operation box |
| CN103571750B (en) * | 2013-07-10 | 2015-06-17 | 温州兆曜机械科技有限公司 | Method for improving inoculation process through aseptic operation box |
| CN104823552A (en) * | 2015-04-13 | 2015-08-12 | 王镱钧 | Seed treatment device and method based on ultraviolet light and ultrasonic mixed synchronization pulse |
| CN104823552B (en) * | 2015-04-13 | 2016-08-17 | 王镱钧 | Device for treatment of seeds based on ultraviolet light and ultrasound wave mixed synchronization pulse and method |
| CN106282009A (en) * | 2016-11-10 | 2017-01-04 | 山东省科学院生态研究所 | A kind of solid materials fermenting device |
| CN106434303A (en) * | 2016-11-18 | 2017-02-22 | 天津开发区合普工贸有限公司 | Bacterial infection experiment device for cell culture dishes |
| CN106434303B (en) * | 2016-11-18 | 2019-04-23 | 天津开发区合普工贸有限公司 | Tissue Culture Dish bacterium infection experimental provision |
| CN106489850A (en) * | 2016-11-30 | 2017-03-15 | 四川省农业科学院蚕业研究所 | Cocoonery is used in a kind of Bombyx Batryticatus production |
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| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20120530 Termination date: 20121019 |