CN106434303B - Tissue Culture Dish bacterium infection experimental provision - Google Patents

Tissue Culture Dish bacterium infection experimental provision Download PDF

Info

Publication number
CN106434303B
CN106434303B CN201611015780.2A CN201611015780A CN106434303B CN 106434303 B CN106434303 B CN 106434303B CN 201611015780 A CN201611015780 A CN 201611015780A CN 106434303 B CN106434303 B CN 106434303B
Authority
CN
China
Prior art keywords
cabin
bacterium infection
bacterium
culture dish
tissue culture
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201611015780.2A
Other languages
Chinese (zh)
Other versions
CN106434303A (en
Inventor
范维林
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Tianjin Hope Industry and Trade Co Ltd
Original Assignee
Tianjin Hope Industry and Trade Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Tianjin Hope Industry and Trade Co Ltd filed Critical Tianjin Hope Industry and Trade Co Ltd
Priority to CN201611015780.2A priority Critical patent/CN106434303B/en
Publication of CN106434303A publication Critical patent/CN106434303A/en
Application granted granted Critical
Publication of CN106434303B publication Critical patent/CN106434303B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/48Holding appliances; Racks; Supports
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/52Mobile; Means for transporting the apparatus
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/04Filters; Permeable or porous membranes or plates, e.g. dialysis
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/20Degassing; Venting; Bubble traps
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/04Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M37/00Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M37/00Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
    • C12M37/02Filters
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature

Abstract

The present invention provides a kind of Tissue Culture Dish bacterium infection experimental provision, including the closed bacterium infection cabin being mounted on movable supporting leg frame, its front has sealing hatch door and hatch door hand lock that can open and close and lock sealing hatch door, there are several cabin inner pallets that can put Tissue Culture Dish on cabin rear wall inside bacterium infection cabin to be exposed in infection experiment environment, bacterium infection cabin top connection atomization diffuser, which will contain germy medical liquid atomizing and diffusing out and be transmitted to Tissue Culture Dish, to carry out exposing infection experiment, bacterium infection cabin lower end is sealably coupled to exhaust processor, the exhaust gas sterilization and high efficiency filter that the circulation of bacterium infection cabin is come, guarantee discharge gas safety discharge, sterilization is carried out with the ultraviolet disinfecting in bacterium infection cabin after the completion of experiment.The beneficial effects of the present invention are: developing, a kind of structure is simple, Tissue Culture Dish bacterium infection experimental provision of safe ready, solves the problems, such as that Tissue Culture Dish be automatically brought into operation and seal off in bacterium infection research work.

Description

Tissue Culture Dish bacterium infection experimental provision
Technical field
The present invention relates to a kind of Tissue Culture Dish bacterium infection experimental provisions.
Background technique
In the scientific research for being related to the fields such as environment, medicine, military affairs, aviation, it is often necessary to Tissue Culture Dish into The experiment of row bacterium infection, the requirement tested to infection environment are very stringent, it is necessary to uniformly culture, no living contaminants, at present to cell The method that culture dish carries out bacterium infection experiment substantially uses artificial dropping liquid, insulating box culture, and the amount of dropping liquid is not in whole process It can guarantee accurate consistent, infection environment is not closed, does not have sterilization measure, therefore have miscellaneous bacteria during the cultivation process and train together It supports and generates, it cannot be guaranteed that the accuracy of the subsequent experimental of the Tissue Culture Dish after bacterium infection, while to the body of operator Also certain harm is generated, the increasingly developed scientific research in presently relevant field and experiment needs is not able to satisfy thus, hinders The deep development of correlative study, in order to fundamentally solve the basic problem of bacterium infection experiment, it is a kind of automatically will be containing thin The medical fluid uniform atomizing of bacterium and diffuse out the Tissue Culture Dish for being transmitted to and being exposed in infection experiment environment carry out expose infection The research and development of experimental facilities are just at inevitable and there is an urgent need to the projects of research and development.
Summary of the invention
To solve the problems, such as above-mentioned technology, it is real that the object of the present invention is to provide a kind of Tissue Culture Dish bacterium infections Experiment device containing germy medical fluid uniform atomizing and will diffuse out the cell for being transmitted to and being exposed in infection experiment environment automatically Culture dish carries out exposing infection experiment, energy automatic disinfection sterilizing after experiment, and filters to the exhaust-gas efficient of generation and guarantee that safety is arranged It puts.
To achieve the above object, the technical solution of the present invention is to provide a kind of Tissue Culture Dish bacterium infection experimental provision, The closed bacterium infection cabin being mounted on movable supporting leg frame including one, front are locked equipped with a sealing hatch door and hatch door hand The movable doorboard that can arbitrarily open and close and lock sealing hatch door, it is evenly distributed on the cabin rear wall inside bacterium infection cabin Several cabin inner pallets are installed, a Tissue Culture Dish can be put on each cabin inner pallet and is allowed to be exposed to infection experiment In environment, bacterium infection cabin top is connected with atomization diffuser, internal can to test as requested equipped with bacterium solution atomizer Required diffuses out containing germy medical liquid atomizing at tiny particulate matter, by the diffusion funnel of bacterium infection cabin upper end It is transmitted to and the Tissue Culture Dish on the cabin inner pallet in being placed in is carried out in bacterium infection cabin to expose infection experiment, bacterium infection The collection funnel of cabin lower end is sealably coupled to exhaust processor, by from the exhaust gas that comes of bacterium infection cabin circulation bacterium and its His solid particle gas sterilization and high efficiency filter guarantee that the exhaust gas of discharge is that sterile innocuous gas can be real with safety dumping By being mounted on the ultraviolet disinfecting on the cabin rear wall both sides inside bacterium infection cabin to entire bacterium infection cabin cabin after the completion of testing Internal portion carries out necessary sterilization.
The beneficial effect is that: in the scientific research for being related to the fields such as environment, medicine, military affairs, aviation, provide one Structure is simple, safe ready, the automatic device for carrying out the experiment of Tissue Culture Dish bacterium infection, it is easy to operate, be fully sealed isolation, Automatic disinfection sterilizing, it is pollution-free, solve Tissue Culture Dish carry out bacterium infection research work in be automatically brought into operation and seal every From problem, filled up this blank.
Detailed description of the invention
Attached drawing 1 is the schematic diagram of structure of the invention.
In Fig. 1:
1. 2. ventilating fan of air intake duct, 3. air inlet funnel, 4. air intake filter 5. is atomized 6. mist of funnel Change diffuser
7. 11. hatch door live axle of liquid inlet conduit 8. liquid feeding pump, 9. inoculum bottle, 10. Sampling Interface, 12. cabin Body rear wall
13. 14. ultraviolet disinfecting 15. of cabin side wall locks 16. hand of handle lock 17. hatch door hand of live axle lock 18. Movable doorboard
19. 22. air exhauster of temperature isolation device 20. high-temperature sterilizing chambers, 21. high efficiency particulate air filter, 23. exhaust gas is discharged Mouthful
24. being vented 25. exhaust processor of air hose, 26. castor, 27. foot brake, 28. lower sleeve 29. collects leakage Bucket
30. 33. upper support leg bar of movable supporting leg assembly 31. fastening wheel, 32. hand latch hook, 34. transparent front wall 35. stabilizer blade flange
36. 37. movable supporting leg frame of fixed platform, 38. hatch door lock ring 39. seals 40. antetheca hatch of hatch door 41. bacterium infection cabin
42. 43. Tissue Culture Dish of cabin inner pallet, 44. hatch door adhesive tape, 45. compressed gas cylinder 46. spreads funnel 47. pressure-control valve
48. 51. bacterium solution atomizer of compression gas tube 49. electricity heating piece, 50. filter screen, 52. two-phase spray head.
Specific embodiment
The present invention is illustrated in conjunction with attached drawing 1 and embodiment.
As shown in Fig. 1, the present invention provides a kind of Tissue Culture Dish bacterium infection experimental provision, including one is mounted on work Closed bacterium infection cabin 41 on dynamic foot-supporting frame 37, bacterium infection cabin 41 are that the smooth middle part of surfaces externally and internally is roomy and nothing It is in gradually taper up the closed cabin of shape that seam, which is connected to upper and lower ends, and the upper end of closed cabin is in the part for gradually tapering up shape Funnel 46 is spread, the contraction upper end of diffusion funnel 46 is joined seamlessly to atomization diffuser 6, and the lower end of closed cabin is in gradually taper up The part of shape is collection funnel 29, and the contraction lower end of collection funnel 29 is joined seamlessly to exhaust processor 25, closed cabin Middle part wide portion is made of the sealed connection of cabin rear wall 12, cabin side wall 13 and transparent front wall 34, the cabin of closed cabin It is evenly distributed on rear wall 12 that several cabin inner pallets 42 are installed, a cell culture can be put on each cabin inner pallet 42 Ware 43 is allowed to be exposed in infection experiment environment, and the both sides of cabin rear wall 12 are equipped with ultraviolet disinfecting 14, the two sides of closed cabin Cabin side wall 13 on be uniformly placed with Sampling Interface 10, equipped with sealing hatch door 39 in the transparent front wall 34 of closed cabin, open Tissue Culture Dish 43 and other articles can be picked and placed, sealing hatch door 39 can be implemented after closing locking for Tissue Culture Dish 43 Bacterium infection experiment;The front in bacterium infection cabin 41 arbitrarily can open and close equipped with a sealing hatch door 39 and hatch door hand lock 17 The movable doorboard 18 of sealing hatch door 39 is closed and locks, sealing hatch door 39 is one by 40 component group of movable doorboard 18 and antetheca hatch At the smooth composite construction of surfaces externally and internally, movable doorboard 18 is the energy as made of smooth outer surface corrosion-resistant rigid material The dodge gate of the planar sheet shape of free open and close sealing, size dimension and antetheca hatch 40 are adapted and are formed to bacterium The closed of cabin 43 is infected, antetheca hatch 40 is the open-mouth ruler on the central location of the transparent front wall 34 in bacterium infection cabin 43 The very little size than movable doorboard 18 is smaller, uniformly installs a circle hatch door adhesive tape 44 in the open circumferential of antetheca hatch 40, big Small size is adapted with movable doorboard 18 to be formed to the closed of bacterium infection cabin 41, and the side of movable doorboard 18 passes through hatch door live axle 11 are connected near the antetheca hatch 40 of 34 central location of transparent front wall in bacterium infection cabin 41, another end edge of movable doorboard 18 Edge equipped with hatch door hand lock 17, hatch door hand lock 17 be made by firm wear-resisting rigid material be used to open or lock dodge gate The two-way complex mechanical mechanisms of plate 18, an end margin of movable doorboard 18 lock live axle 16 by hand and are connected to hatch door hand lock 17, cabin There is the hand latch hook 32 of manual lock handle 15 and rigid material production on Men Shousuo 17, antetheca hatch 40 is nearby fixedly mounted The hatch door lock ring 38 for the single side opening for having rigid material to make, can constrain the hand latch hook 32 on habitation Men Shousuo 17, rotatable cabin Lock handle 15 on Men Shousuo 17 can arbitrarily open and close and lock movable doorboard 18, and movable doorboard 18 compresses hatch adhesive tape 37, which can close antetheca hatch 40, forms to the closed of bacterium infection cabin 41;41 top of bacterium infection cabin is connected with atomization diffusion Device 6, atomization diffuser 6 is the atomization generation being made by firm resistant material and disperser, main body are one The smooth hemispheric atomization funnel 5 of back-off of the surfaces externally and internally of a light sheet material production, atomization funnel 5 are joined seamlessly to below The contraction upper end of the diffusion funnel 46 in bacterium infection cabin 41, the top of atomization funnel 5 are seamlessly connected the one of air intake duct 1 End, the other end of air intake duct 1 are connected to air inlet funnel 3, ventilating fan 2 and air intake filter are equipped in air inlet funnel 3 4, fresh air can be conveyed into bacterium infection cabin 41 at any time, is atomized among funnel 5 and bacterium solution atomizer 51, bacterium solution mist is installed Changing device 51 is the gas-liquid two-phase flow atomization device with heating function for being fixedly mounted on 6 center of atomization diffuser, atomization Two-phase spray head 52 is installed, the bottom of two-phase spray head 52 is that an electricity heating piece 49 is able to maintain temperature, two-phase spray among device First 52 top is a filter screen 50, can filter out the suitable atomization gas of granular size that experiment needs, be mounted on mist The compressed gas cylinder 45 for changing 5 one end of funnel passes through the control of pressure-control valve 47, and the compressed gas with certain pressure is via compression Airway 48 is transported to the air inlet of two-phase spray head 52, and the liquid feeding pump 8 for being mounted on atomization 5 other end of funnel passes through liquid inlet conduit 7 Inoculum in inoculum bottle 9 is transported to the inlet of two-phase spray head 52, compressed gas and inoculum exist Two-phase spray head 52 meets, and forms atomization under venturi principle effect and produces containing germy infection gas, bacterium solution atomizer 51 will can diffuse out containing germy medical liquid atomizing at tiny particulate matter needed for experiment as requested, by bacterium The diffusion funnel 46 of infection 41 upper end of cabin, which is transmitted in bacterium infection cabin 41, trains the cell on the cabin inner pallet 42 in being placed in Ware 43 is supported to carry out exposing infection experiment;The collection funnel 29 of 41 lower end of bacterium infection cabin is sealably coupled to exhaust processor 25, gives up Treatment apparatus 25 is one and is made the cylindrical units of closed venting channels by corrosion-resistant and high-temperature resistant material, cylindrical unit it is upper End is sealably coupled to the end of the collection funnel 29 in bacterium infection cabin 41, and the one end being tightly connected with collection funnel 29 is one and fills out Filling glass fibre class has ventilative and isolation temperature function temperature isolation device 19, in the following connection one of temperature isolation device 19 High-temperature sterilizing chambers 20, high-temperature sterilizing chambers 20 be by arranged with gap electronic heating device production be sealingly mounted at one it is close A gas flow circuits are formed in the high-temperature resistant container closed, and there are the waste exhaust gases of carrying disease germs to come that will circulate to be heated into High Temperature Gas Body completes the sterilization to exhaust gas, is equipped with the high efficiency particulate air filter being made by special filter membrane below high-temperature sterilizing chambers 20 21, the bacterium in exhaust gas is gone out to guarantee that the exhaust gas of discharge is sterile innocuous gas with other solid particulate filters, in cylindrical unit End is the outlet port 23 for being equipped with air exhauster 22, by from bacterium infection cabin 41 circulate bacterium in the exhaust gas that comes and Other solid particle gas sterilizations and high efficiency filter guarantee that the exhaust gas of discharge is that sterile innocuous gas can the row of suction at any time Out by processing can safety dumping, by being mounted on 12 both sides of cabin rear wall inside bacterium infection cabin 41 after the completion of experiment Ultraviolet disinfecting 14 is to the necessary sterilization of progress inside entire 41 cabin of bacterium infection cabin;Movable supporting leg frame 37 is by firm Fixed rack device that is that load-bearing rigid material is made and can arbitrarily moving, including one be horizontally mounted intermediate placement It fixes the fixed platform 36 for carrying the bacterium infection cabin 41 of entire Tissue Culture Dish bacterium infection experimental provision and four is mounted on The quadrangle of fixed platform 36 can adjust separately 36 height of fixed platform and moveable support leg assembly 30, each activity The stabilizer blade flange 35 that the top of support leg assembly 30 has a metal material to make, is fixedly installed to fixed platform 36 A close edge of table top lower surface of base plate, 35 intermediate vertical of stabilizer blade flange fixes a upper support leg bar 33, in upper support leg The lower end of bar 33 is cased with one section of lower sleeve 28, and 28 top of lower sleeve is fixed with fastening wheel 31, when unclamping fastening wheel 31, it will be able to Change the length that upper support leg bar 33 is inserted between lower sleeve 28, the height of movable supporting leg assembly 30 is also just changed, when tightening When fastening wheel 31, the height of movable supporting leg assembly 30 is fixed motionless, the lower end of each lower sleeve 28 connects one and has The castor 26 of foot brake 27, since the bacterium infection cabin 41 of entire Tissue Culture Dish bacterium infection experimental provision is fixedly installed to In fixed platform 36, the quadrangle of the table top lower surface of base plate of fixed platform 36 is separately fixed at four movable supporting leg assemblies 30 On, so the movable supporting leg assembly 30 of adjustment quadrangle, it will be able to adjust entire Tissue Culture Dish bacterium infection experimental provision The height and flatness in bacterium infection cabin 41.
Embodiment:
In use, being ready to test inoculum used first and pouring into inoculum bottle 9, it gets out this reality Several Tissue Culture Dish 43 tested check whether the compressed gas in compressed gas cylinder 45 is enough, then by Tissue Culture Dish Bacterium infection experimental provision is placed to the place needed using this device, according to the demand of operator by adjusting movable supporting leg frame 37 adjust the height and levelness of Tissue Culture Dish bacterium infection experimental provision, and adjusting is fixed on 36 lower surface four of fixed platform Four movable supporting leg assemblies 30 that a angle is nearby installed unclamp the fastening wheel 31 in movable supporting leg assembly 30, according to suitable Level Change upper support leg bar 33 be inserted into lower sleeve 28 between length, thus change entire movable supporting leg assembly 30 Highly, reach when tightening fastening wheel 28 after required height, at this moment the height of movable supporting leg assembly 30 is fixed motionless, successively Four movable supporting leg assemblies 30 are adjusted separately into sustained height, then by the lower sleeve of each movable supporting leg assembly 30 The foot brake 27 of the castor 26 of 28 lower ends is checked, and the height and flatness for maintaining like entire experimental provision and cannot be moved It is dynamic;The sealing hatch door 39 in the transparent front wall 34 in bacterium infection cabin 41 is opened now, rotates the lock hand on hatch door hand lock 17 by hand Handle 15 detaches the hand latch hook 32 on hatch door hand lock 17 from hatch door lock ring 38, thus opens sealing hatch door 39 Several ready Tissue Culture Dish 43 are successively placed in evenly distributed several on cabin rear wall 12 by movable doorboard 18 On cabin inner pallet 42, a Tissue Culture Dish 43 can be put on each cabin inner pallet 42 and is allowed to be exposed to infection experiment environment In;Movable doorboard 18 is closed, rotates the lock handle 15 on hatch door hand lock 17 by hand, enters the hand latch hook 32 on hatch door hand lock 17 Hatch door lock ring 38 simultaneously constrains latch hook 32 of stopping, and such movable doorboard 18 just compresses hatch adhesive tape 37 and closes 34 center of transparent front wall Antetheca hatch 40 on position ensure that the closed of bacterium infection cabin 41;Open atomization 5 one end of funnel of atomization diffuser 6 The pressure-control valve 47 of compressed gas cylinder 45 makes the compressed gas with the pressure for meeting requirement of experiment via 48 quilt of compression gas tube It is transported to the air inlet of two-phase spray head 52, opens the liquid feeding pump 8 of atomization 5 other end of funnel, the bacterium training in inoculum bottle 9 Nutrient solution is transported to the inlet of two-phase spray head 52 by liquid inlet conduit 7, and compressed gas and inoculum are in two-phase spray head 52 It meets, the electricity heating piece 49 of 52 bottom of two-phase spray head remains required temperature, and the filter screen 50 at 52 top of two-phase spray head screens The suitable atomization gas of granular size for testing needs out forms atomization under venturi principle effect and produces containing germy Gas is infected, germy medical fluid will will be contained needed for experiment as requested by being atomized the bacterium solution atomizer 51 installed among funnel 5 It is atomized into tiny particulate matter to diffuse out, is transmitted to bacterium infection cabin by the diffusion funnel 46 of 41 upper end of bacterium infection cabin Start expose infection experiment to the Tissue Culture Dish 43 on the cabin inner pallets 42 in being placed in 41, it, can in experimentation Evenly arranged Sampling Interface 10 is sampled on cabin side wall 13 to pass through bacterium infection cabin 41 at any time;It is thin in experimentation The exhaust gas that the bacterium infection circulation of cabin 41 comes is transmitted to exhaust processor 25 through the collection funnel 29 of closed cabin lower end, passes through first It is that a filled glass fiber class has ventilative and isolation temperature function temperature isolation device 19, then passes through the height connected below The waste exhaust gases of carrying disease germs that circulation comes are heated into high-temperature gas to complete the sterilization to exhaust gas by warm sterilizer 20, then are passed through The high efficiency particulate air filter 21 being made by special filter membrane that high-temperature sterilizing chambers 20 are installed below, by exhaust gas bacterium and other solids Grain, which filters out, guarantees that the exhaust gas of discharge is sterile innocuous gas, most the connection air exhauster 22 through 25 end of exhaust processor afterwards Outlet port 23, bacterium and other solid particle gas sterilizations that will be circulated in the exhaust gas that comes from bacterium infection cabin 41 And high efficiency filter, guarantee that the exhaust gas of discharge is sterile innocuous gas, aspirates safety dumping at any time;Experiment is completed, and compressed gas is closed The pressure-control valve 47 and liquid feeding pump 8 of bottle 45, bacterium solution atomizer 51 stop diffusion and transmit containing the tiny of germy medical fluid Particulate matter opens the sealing hatch door 39 in the transparent front wall 34 in bacterium infection cabin 41, rotates the lock on hatch door hand lock 17 by hand Handle 15 detaches the hand latch hook 32 on hatch door hand lock 17 from hatch door lock ring 38, thus opens sealing hatch door 39 Movable doorboard 18, several Tissue Culture Dish 43 of bacterium infection experiment will be completed successively from being placed on cabin rear wall 12 Bacterium infection cabin 41 is taken out on several cabin inner pallets 42, is then shut off movable doorboard 18, is rotated by hand on hatch door hand lock 17 Handle 15 is locked, the hand latch hook 32 on hatch door hand lock 17 is made to enter hatch door lock ring 38 and constrains latch hook 32 of stopping, is shut off so simultaneously The movable doorboard 18 for having locked sealing hatch door 39, then by being mounted on 12 both sides of cabin rear wall inside bacterium infection cabin 41 Ultraviolet disinfecting 14 is to progress sterilization inside entire 41 cabin of bacterium infection cabin;By the bacterium training in inoculum bottle 9 Nutrient solution is emptied, and the foot brake 27 of the castor 26 of four movable supporting leg assemblies 30 of movable supporting leg frame 37 is then unclamped, by cell Culture dish bacterium infection experimental provision shifts the place for storing this device onto case experiment next time uses;The process bacterium infection of taking-up The Tissue Culture Dish 43 of experiment carries out the scientific research and application of next step.

Claims (4)

1. a kind of Tissue Culture Dish bacterium infection experimental provision is mounted on movable supporting leg frame (37) it is characterized in that: including one Closed bacterium infection cabin (41), bacterium infection cabin (41) are that the smooth middle part of surfaces externally and internally is roomy and be seamlessly connected Upper and lower ends are in gradually taper up the closed cabin of shape, and the middle part wide portion of closed cabin is by cabin rear wall (12), cabin Side wall (13) and transparent front wall (34) are tightly connected composition, and the upper end of closed cabin is diffusion leakage in the part for gradually tapering up shape It struggles against (46), the contraction upper end of diffusion funnel (46) is joined seamlessly to atomization diffuser (6), and the lower end of closed cabin is in gradually taper up The part of shape is collection funnel (29), is uniformly placed with Sampling Interface on the cabin side wall (13) of the two sides of closed cabin (10), the front of bacterium infection cabin (41) can arbitrarily be opened and closed equipped with a sealing hatch door (39) and hatch door hand lock (17) And the movable doorboard (18) of sealing hatch door (39) is locked, evenly distributed peace on bacterium infection cabin (41) internal cabin rear wall (12) Equipped with several cabin inner pallets (42), a Tissue Culture Dish (43) can be put on each cabin inner pallet (42), atomization is expanded Scattered device (6) are the atomization generations being made by firm resistant material and disperser, main body are a light sheets Expect the smooth hemispheric atomization funnel (5) of back-off of the surfaces externally and internally of production, atomization funnel (5) is joined seamlessly to bacterium below The contraction upper end of the diffusion funnel (46) of cabin (41) is infected, the top of atomization funnel (5) is seamlessly connected air intake duct (1) One end, the other end of air intake duct (1) are connected to air inlet funnel (3), be equipped in air inlet funnel (3) ventilating fan (2) and Air intake filter (4) can convey fresh air into bacterium infection cabin (41) at any time, bacterium is housed among atomization diffuser (6) Liquid atomization device (51), bacterium solution atomizer (51) be one be fixedly mounted on atomization diffuser (6) center have heating function Gas-liquid two-phase flow atomization device is equipped with two-phase spray head (52) among atomising device, and the bottom of two-phase spray head (52) is an electricity Heating piece (49) is able to maintain temperature, is that a filter screen (50) can filter out experiment needs at the top of two-phase spray head (52) The suitable atomization gas of granular size, the compressed gas cylinder (45) for being mounted on atomization funnel (5) one end pass through pressure-control valve (47) Control, the compressed gas with certain pressure is transported to the air inlet of two-phase spray head (52) via compression gas tube (48), pacifies Pass through liquid inlet conduit (7) for the Bacteria Culture in inoculum bottle (9) mounted in the liquid feeding pump (8) of atomization funnel (5) other end Liquid is transported to the inlet of two-phase spray head (52), compressed gas and inoculum and meets in two-phase spray head (52), in venturi The lower formation atomization of principle effect is produced containing germy infection gas, by the diffusion funnel of bacterium infection cabin (41) upper end (46) it is transmitted in bacterium infection cabin (41) and the Tissue Culture Dish (43) on the cabin inner pallet (42) in being placed in is exposed Infection experiment, by being mounted on the ultraviolet disinfecting on internal cabin rear wall (12) both sides in bacterium infection cabin (41) after the completion of experiment (14) to necessary sterilization is carried out inside entire bacterium infection cabin (41) cabin, the contraction lower end of collection funnel (29) is seamless It is connected to exhaust processor (25), exhaust processor (25) is one logical by the closed ventilation that is made of corrosion-resistant and high-temperature resistant material The cylindrical unit in road, the upper end of cylindrical unit is sealably coupled to the end of the collection funnel (29) of bacterium infection cabin (41), with receipts One end that collection funnel (29) is tightly connected is that a filled glass fiber class has ventilative and isolation temperature function temperature isolation Device (19) connects a high-temperature sterilizing chambers (20) in temperature isolation device (19) below, and high-temperature sterilizing chambers (20) are by with gap The electronic heating device production of arrangement is sealingly mounted at one gas flow circuits of formation in a closed high-temperature resistant container, High-temperature gas is heated into the waste exhaust gases of carrying disease germs to come that will circulate to complete the sterilization to exhaust gas, in high-temperature sterilizing chambers (20) high efficiency particulate air filter (21) being made by special filter membrane is installed below, by exhaust gas bacterium and other solid particle mistakes It filters out and guarantees that the exhaust gas of discharge is sterile innocuous gas, be the exhaust gas discharge for being equipped with air exhauster (22) in cylindrical unit end The exhaust gas of safety dumping is capable of in mouth (23), at any time suction discharge by processing.
2. Tissue Culture Dish bacterium infection experimental provision according to claim 1, it is characterized in that: the sealing hatch door (39) It is the smooth composite construction of a surfaces externally and internally being made of movable doorboard (18) and antetheca hatch (40) component, movable doorboard (18) being can the planar sheet shape that seals of free open and close as made of the corrosion-resistant rigid material of smooth outer surface Dodge gate, size dimension and antetheca hatch (40), which are adapted, to be formed to the closed of bacterium infection cabin (41), antetheca hatch (40) It is ruler of the opening size than movable doorboard (18) on the central location of the transparent front wall (34) of bacterium infection cabin (41) It is very little smaller, one circle hatch door adhesive tape (44), size dimension and dodge gate are uniformly installed in the open circumferential of antetheca hatch (40) Plate (18), which is adapted, to be formed to the closed of bacterium infection cabin (41), and the side of movable doorboard (18) is connected by hatch door live axle (11) To near the antetheca hatch (40) of transparent front wall (34) central location of bacterium infection cabin (41), the other end of movable doorboard (18) Edge can be opened and closed arbitrarily and be locked movable doorboard (18) equipped with hatch door hand lock (17), rotation hatch door hand lock (17), living Dynamic door-plate (18), which compress hatch adhesive tape (44), can close antetheca hatch (40) formation to the closed of bacterium infection cabin (41).
3. Tissue Culture Dish bacterium infection experimental provision according to claim 1, it is characterized in that: hatch door hand lock (17) Be be made by firm wear-resisting rigid material be used to open or lock the two-way complex mechanical mechanisms of movable doorboard (18), it is living One end margin of dynamic door-plate (18) is connected to hatch door hand lock (17) by hand lock live axle (16), and hatch door hand locks useful hand behaviour on (17) The hand latch hook (32) of lock handle (15) and the rigid material production of work, antetheca hatch (40) are nearby fixedly installed with rigid material system The hatch door lock ring (38) of the single side opening of work can constrain the hand latch hook (32) on habitation Men Shousuo (17), rotation hatch door hand lock (17) the lock handle (15) on can be opened and closed arbitrarily and lock movable doorboard (18).
4. Tissue Culture Dish bacterium infection experimental provision according to claim 1, it is characterized in that: the movable supporting leg frame It (37) is fixed rack device that is being made by firm load-bearing rigid material and can arbitrarily moving, including a horizontal peace Install the intermediate fixed platform for placing the fixed bacterium infection cabin (41) for carrying entire Tissue Culture Dish bacterium infection experimental provision (36) and four quadrangles for being mounted on fixed platform (36) can adjust separately fixed platform (36) height and moveable branch Support leg component (30), the stabilizer blade flange that the top of each movable supporting leg assembly (30) has a metal material to make (35), a close edge of the table top lower surface of base plate of fixed platform (36) is fixedly installed to, in stabilizer blade flange (35) Between vertically fix a upper support leg bar (33), be cased with one section of lower sleeve (28), lower sleeve (28) in the lower end of upper support leg bar (33) Top is fixed with fastening wheel (31), when unclamping fastening wheel (31), it will be able to change upper support leg bar (33) insertion lower sleeve (28) Between length, also just change the height of movable supporting leg assembly (30), when tightening fastening wheel (31), movable supporting leg group The height of part (30) is fixed motionless, one castor with foot brake (27) of lower end connection of each lower sleeve (28) (26), since the bacterium infection cabin (41) of entire Tissue Culture Dish bacterium infection experimental provision is fixedly installed to fixed platform (36) on, the quadrangle of the table top lower surface of base plate of fixed platform (36) is separately fixed on four movable supporting leg assemblies (30), So the movable supporting leg assembly (30) of adjustment quadrangle, it will be able to adjust the thin of entire Tissue Culture Dish bacterium infection experimental provision Bacterium infects the height and flatness of cabin (41).
CN201611015780.2A 2016-11-18 2016-11-18 Tissue Culture Dish bacterium infection experimental provision Active CN106434303B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201611015780.2A CN106434303B (en) 2016-11-18 2016-11-18 Tissue Culture Dish bacterium infection experimental provision

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201611015780.2A CN106434303B (en) 2016-11-18 2016-11-18 Tissue Culture Dish bacterium infection experimental provision

Publications (2)

Publication Number Publication Date
CN106434303A CN106434303A (en) 2017-02-22
CN106434303B true CN106434303B (en) 2019-04-23

Family

ID=58220289

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201611015780.2A Active CN106434303B (en) 2016-11-18 2016-11-18 Tissue Culture Dish bacterium infection experimental provision

Country Status (1)

Country Link
CN (1) CN106434303B (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112662522B (en) * 2021-01-22 2022-06-21 湘西州干细胞库医疗科技有限公司 A atomizing device for cell culture liquid

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN200970564Y (en) * 2006-11-18 2007-11-07 陈盾 Venturi atomizer
CN202173948U (en) * 2011-08-16 2012-03-28 天津开发区合普工贸有限公司 Explosion-proof liquid and gas phase aerosol exposure device
CN202246691U (en) * 2011-10-19 2012-05-30 东北石油大学 Ultrasonic atomizing type aseptic inoculation box
CN102670328A (en) * 2012-05-29 2012-09-19 天津开发区合普工贸有限公司 Pathogenic bacteria inhalation toxicology experimental equipment for respiratory system
CN204134652U (en) * 2014-08-29 2015-02-04 中国人民解放军军事医学科学院军事兽医研究所 A kind of biopathogen contact transmission and aerosol transmission experimental rig
CN104862210A (en) * 2015-04-29 2015-08-26 李郝 Microbiological detection cultivating device
CN206266597U (en) * 2016-11-18 2017-06-20 天津开发区合普工贸有限公司 Tissue Culture Dish bacterium infection experimental provision

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN200970564Y (en) * 2006-11-18 2007-11-07 陈盾 Venturi atomizer
CN202173948U (en) * 2011-08-16 2012-03-28 天津开发区合普工贸有限公司 Explosion-proof liquid and gas phase aerosol exposure device
CN202246691U (en) * 2011-10-19 2012-05-30 东北石油大学 Ultrasonic atomizing type aseptic inoculation box
CN102670328A (en) * 2012-05-29 2012-09-19 天津开发区合普工贸有限公司 Pathogenic bacteria inhalation toxicology experimental equipment for respiratory system
CN204134652U (en) * 2014-08-29 2015-02-04 中国人民解放军军事医学科学院军事兽医研究所 A kind of biopathogen contact transmission and aerosol transmission experimental rig
CN104862210A (en) * 2015-04-29 2015-08-26 李郝 Microbiological detection cultivating device
CN206266597U (en) * 2016-11-18 2017-06-20 天津开发区合普工贸有限公司 Tissue Culture Dish bacterium infection experimental provision

Also Published As

Publication number Publication date
CN106434303A (en) 2017-02-22

Similar Documents

Publication Publication Date Title
JP5823727B2 (en) Isolator
CN106434303B (en) Tissue Culture Dish bacterium infection experimental provision
CN205982221U (en) Smell, VOC and showy harmful substance testing arrangement
CN211793596U (en) Laboratory is with mouse particulate matter expose cabin device
CN206266597U (en) Tissue Culture Dish bacterium infection experimental provision
CN107917824A (en) A kind of low pressure in minienvironment cabin adjusts the method for sampling and device
CN104805012A (en) Automatic cell culture box
CN205982222U (en) Smell, VOC and showy harmful substance checkout room with outer circulating filter apparatus
CN103805499B (en) Air sterilization effect pipeline evaluation system
CN105909000B (en) Between the sterile gas lock of the stream of people/logistics
CN207913788U (en) a kind of bio-safety isolating device
CN208355600U (en) The big animal organism aerosol mouth and nose exposure system of biological safe type primate
CN105486817B (en) A kind of assay method of material to the non-adsorbed purifying property of air pollutants
CN205310303U (en) Sterility testing technology isolator of two -sided laminar flow operation
CN210434542U (en) Clinical laboratory uses anti-pollution storage device
CN106239568A (en) Biological safe type fowl negative pressure isolator
CN206808513U (en) Plant inspection quarantine station device for fumigation
CN206526302U (en) Portable fumigation machine
CN207866781U (en) A kind of harmful substance test device with inner air circulator
CN207519217U (en) A kind of device for studying allelopathy activity
CN214881586U (en) Cell in-vitro culture system
CN205860664U (en) The aseptic freeze-dried separator system of use for laboratory medicine
CN208757609U (en) A kind of air cleaning system for embryo experiments room
CN204601229U (en) Between dosing and automatic control system
CN207520765U (en) A kind of activated carbon weighing room air cleaning unit

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant