CN220183296U - Cell culture device for cell engineering - Google Patents
Cell culture device for cell engineering Download PDFInfo
- Publication number
- CN220183296U CN220183296U CN202321736205.7U CN202321736205U CN220183296U CN 220183296 U CN220183296 U CN 220183296U CN 202321736205 U CN202321736205 U CN 202321736205U CN 220183296 U CN220183296 U CN 220183296U
- Authority
- CN
- China
- Prior art keywords
- cabin
- box body
- cell
- ultraviolet lamp
- lamp group
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000004113 cell culture Methods 0.000 title claims abstract description 17
- 238000007789 sealing Methods 0.000 claims abstract description 48
- 230000007246 mechanism Effects 0.000 claims abstract description 22
- 239000007788 liquid Substances 0.000 claims abstract description 21
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 7
- 238000005192 partition Methods 0.000 claims description 5
- 239000007921 spray Substances 0.000 claims description 5
- 238000002347 injection Methods 0.000 claims description 4
- 239000007924 injection Substances 0.000 claims description 4
- 230000000249 desinfective effect Effects 0.000 claims description 2
- 238000005485 electric heating Methods 0.000 claims description 2
- 238000009928 pasteurization Methods 0.000 claims description 2
- 206010044625 Trichorrhexis Diseases 0.000 claims 1
- 238000004659 sterilization and disinfection Methods 0.000 abstract description 24
- 230000001954 sterilising effect Effects 0.000 abstract description 14
- 238000004140 cleaning Methods 0.000 abstract description 9
- 241000894006 Bacteria Species 0.000 abstract description 5
- 230000010261 cell growth Effects 0.000 abstract description 3
- 238000002360 preparation method Methods 0.000 abstract description 2
- 210000004027 cell Anatomy 0.000 description 12
- 238000004891 communication Methods 0.000 description 6
- 238000004321 preservation Methods 0.000 description 6
- 238000010438 heat treatment Methods 0.000 description 3
- 230000007547 defect Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 206010011409 Cross infection Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 206010029803 Nosocomial infection Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 238000012832 cell culture technique Methods 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 210000004748 cultured cell Anatomy 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000036512 infertility Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 239000008400 supply water Substances 0.000 description 1
- 230000001360 synchronised effect Effects 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/25—Greenhouse technology, e.g. cooling systems therefor
Abstract
The utility model discloses a cell culture device for cell engineering, which comprises a box body, wherein an inner sealing mechanism and an outer sealing mechanism for sealing are arranged at the box body, a plurality of trays for placing culture dishes are fixed in the box body, ultraviolet lamp groups for disinfection are arranged on the inner wall of the box body, and a gas-liquid circulation mechanism for high-temperature sterilization is arranged in the box body; on the sealed function and disinfection before the operation, adopt inside and outside two sealing mechanism, under the prerequisite that has good leakproofness, when the user opens interior sealing door, must pass through the disinfection of first ultraviolet lamp group, can not carry outside bacterium when guaranteeing the operation, influence the normal growth of cell, on the disinfection function after the operation, adopt high temperature gas to disinfect and high temperature liquid abluent two kinds of cleaning modes, to cultivate after finishing to carry out multiple disinfection, stoving, guarantee cultivate the inside aseptic, the non-stain environment of cabin, in preparation for the use of next cultivation work.
Description
Technical Field
The utility model belongs to the field of cell culture equipment, and particularly relates to a cell culture device for cell engineering.
Background
Cell culture techniques refer to the growth of cells under in vitro conditions, during which the cells no longer form tissue (animals). The culture is a single cell or a population of cells. Cells are grown in an artificial environment, and due to the change of the environment, the movement of the cells or the influence of other factors, the culture time is prolonged, the cells are single-type due to passage, and the cell culture is often operated by using a cell culture bin.
The Chinese patent with the publication number of CN216192393U discloses a cell culture device for cell engineering, which comprises a shell, ultraviolet lamps and a control mechanism for sealing, wherein after a drawing plate is opened, the control mechanism is pressed, so that the shell is opened, the inside of the shell is subjected to subsequent operation, and the function of aseptic operation is achieved by using ultraviolet rays and the like to irradiate before a worker opens the shell.
The technical scheme has the following defects: although the sterilization device has the characteristic of actively preventing external bacteria from entering, the sterilization device does not have a sterilization function for the interior of the box body, and the sterilization and cleaning for the next use are considered for the growth of harmful microorganisms in the interior and the sanitation of the growth environment of cells in the interior.
Disclosure of Invention
Aiming at the defects, the utility model provides a cell culture device for cell engineering, which comprises a box body, wherein an inner sealing mechanism and an outer sealing mechanism for sealing are arranged at the box body, a plurality of trays for placing culture dishes are fixed inside the box body, an ultraviolet lamp group for disinfection is arranged on the inner wall of the box body, and a gas-liquid circulation mechanism for high-temperature sterilization is arranged inside the box body.
Further, sealing mechanism includes outer sealing door and interior sealing door, and outer sealing door passes through the hinge and articulates in the opening part of box, and outer sealing door's outer lane is fixed with sealed the pad, and interior sealing door includes two and articulates in the inside door body of box and seals the inside of box for cultivateing the cabin through the round pin axle, and two door body do not are equipped with synchronous syntropy same speed driven motor, and the bottom of box is equipped with the control switch that is used for controlling the motor.
Through above-mentioned technical scheme, can realize the bilayer to the box sealed, must carry out ultraviolet lamp disinfection operation when carrying out the operation of unpacking simultaneously, guarantee inside aseptic environment.
Further, the ultraviolet lamp group includes first ultraviolet lamp group and second ultraviolet lamp group, and first ultraviolet lamp group installs in the interior diapire and the interior roof of box and is located between outer seal door and the interior seal door, and the second ultraviolet lamp group installs in the inner wall in cultivation cabin.
Through the technical scheme, the sterilization by ultraviolet rays is realized.
Further, the gas-liquid circulation mechanism is including seting up in the basin of cultivateing the indoor diapire of cabin, install in the pump machine of cultivateing cabin terminal angle department and be used for the circulating pipe, waterproof type electrothermal tube is installed to the bottom of basin, the inner wall of basin is separated into gas storehouse and liquid storehouse through fixed baffle, the bottom fixed intercommunication in liquid storehouse has the injection valve, the circulating pipe encircles to the inside of cultivateing the cabin, and the top joint is inside the draw-in groove of cultivateing the indoor roof of cabin seting up, the port intercommunication in the left side of circulating pipe has first three-way pipe, the port on the right side of circulating pipe communicates with the output of pump machine, the input intercommunication of pump machine has the second three-way pipe, two reposition of redundant personnel ends of first three-way pipe and second three-way pipe are located the inside in gas storehouse and liquid storehouse respectively.
Through the technical scheme, the high-temperature disinfection and sterilization of the internal environment can be realized.
Further, the inner ring of the circulating pipe is fixedly embedded with symmetrical communication valves, two communication valves positioned at the same horizontal position are communicated through an adding pipe, and spray heads distributed at equal distances are fixedly communicated at the bottom end of the adding pipe.
Through the technical scheme, the high-temperature cleaning work can be realized, the cleaning process is carried out before the next use, and the sterile and pollution-free environment during use is ensured.
Further, the tray is located inside the culture cabin, the middle part of the tray is provided with partition type bulges, and the middle parts of the left side and the right side of the tray are penetrated by the circulating pipe.
Through above-mentioned technical scheme, play the effect of separation, can adjust the interval of tray according to the position needs simultaneously.
Further, the inner wall of the box body is inlaid with an insulating layer.
Through the technical scheme, even under the condition of power failure, the inside can also guarantee the high-temperature environment for a certain time.
Compared with the prior art, the utility model has the following beneficial effects:
1. sealing and sterilizing functions prior to operation: the inner sealing mechanism and the outer sealing mechanism are adopted, so that on the premise of good sealing performance, when a user opens the inner sealing door, the inner sealing door is killed by the first ultraviolet lamp group, and external bacteria cannot be carried in the operation process, so that the normal growth of cells is influenced;
2. in terms of the disinfection function after operation: the two cleaning modes of high-temperature gas sterilization and high-temperature liquid cleaning are adopted, and multiple sterilization and drying are carried out after the culture is finished, so that the sterile and non-stain environment in the culture cabin is ensured, and the culture cabin is prepared for the next culture work.
Drawings
FIG. 1 is a schematic diagram of the present utility model.
Fig. 2 is a schematic view of the present utility model in an opened state of the outside sealing door.
Fig. 3 is a cross-sectional view of the case of the present utility model.
In the figure: the device comprises a box body 1, an outer sealing door 2, an inner sealing door 3, a water tank 41, a partition plate 42, a pump 43, a circulating pipe 44, an injection valve 45, a first three-way pipe 46, a second three-way pipe 47, a tray 5, a first ultraviolet lamp group 61, a second ultraviolet lamp group 62, a heat preservation layer 7, a control switch 8, a communication valve 91, a loading pipe 92 and a spray head 93.
Detailed Description
In order to facilitate an understanding of the utility model, the device of the utility model will be described more fully below with reference to the accompanying drawings. Examples of the apparatus are given in the accompanying drawings. However, the apparatus may be embodied in many different forms and is not limited to the embodiments described herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.
Examples
As shown in fig. 1-3, this embodiment provides a cell culture apparatus for cell engineering, including box 1, box 1 department is equipped with the inside and outside sealing mechanism that is used for sealedly, through inside and outside double seal, holistic leakproofness has been increased, guarantee the sterility of inside culture environment, the inside of box 1 is fixed with the tray 5 that a plurality of is used for placing the culture dish, the ultraviolet lamp group that is used for sterile is installed to the inner wall of box 1, be used for all disinfecting before entering the staff's hand and get into after, prevent that bacterium from getting into the inside gas-liquid circulation mechanism that causes the influence to the cultured cells of cultivation cabin, the internally mounted of box 1 has the function that is used for pasteurization, can select high temperature disinfection or high temperature water cleaning through needs, guarantee that box 1 can disinfect and keep sterile environment after using, in preparation for next time. The inner wall of the box body 1 is inlaid with a heat preservation layer 7, when the heat preservation layer 7 is used for high-temperature sterilization, the dissipation of internal heat is reduced, even under the condition of power failure, the internal part can still keep a high-temperature environment for a certain time, the subsequent sterilization treatment is carried out, the heat preservation layer 7 can be made of traditional heat preservation materials, and can also be set into the environment of a vacuum tank, and the heat preservation layer can be selected according to the needs.
The sealing mechanism comprises an outer sealing door 2 and an inner sealing door 3, the outer sealing door 2 is hinged to an opening of a box body 1 through a hinge, a control panel is arranged on the front face of the outer sealing door 2, monitoring and control are conveniently carried out on internal equipment and environment (electromagnetic valve, pump, ultraviolet lamp set and electric heating tube are conveniently controlled, a temperature and humidity detector can be arranged in the box body as required, the temperature and humidity detector is conveniently displayed at the panel), a sealing gasket is fixed on an outer ring of the outer sealing door 2 for improving tightness (adopting medical rubber materials), the inner sealing door 3 comprises two door bodies hinged to the inside of the box body 1 through a pin shaft and sealing the inside of the box body 1 into a culture cabin, motors (adopting high-temperature resistant waterproof type) which are synchronously driven at the same direction and the same speed are respectively arranged at the two door bodies, a control switch 8 (using a contact switch can be arranged at the bottom and can be opened through the foot part), and what is required is stated is that when the outer sealing door 2 is in a non-sterile environment, the automatic driving can be carried out through the motor, and the hands are prevented from being polluted after the workers touch the outer sealing door 2.
The ultraviolet lamp group includes first ultraviolet lamp group 61 and second ultraviolet lamp group 62, first ultraviolet lamp group 61 installs in interior bottom wall and the interior roof of box 1 and is located between outer seal door 2 and the interior seal door 3, second ultraviolet lamp group 62 installs in the inner wall of cultivateing the cabin, two sets of ultraviolet lamps correspond two respectively and disinfect the work, first ultraviolet lamp group 61 is used for opening outer seal door 2 promptly, get into and cultivate the cabin before initiatively disinfect, prevent to bring in external bacterium, and second ultraviolet lamp group 62 is used for carrying out the disinfection treatment to cultivate the cabin after wasing, the secondary.
The gas-liquid circulation mechanism is including seting up in the basin 41 of cultivateing the indoor diapire of cabin, install in the pump 43 of cultivateing cabin terminal angle department and be used for circulating pipe 44, waterproof type electrothermal tube is installed to the bottom of basin 41, and the electrothermal tube is adjustable, can be used for the control of temperature, cultivate (constant temperature) through setting up different temperatures, wash (warm) and stoving sterilization (high temperature) work, to the transmission of high temperature, the accessible self-heating, heat inside air, conveniently carry out high temperature gas at this moment and transmit at circulating pipe 44, carry out high temperature stoving in the cultivateing cabin, its heating also can heat the water source in the bottom basin simultaneously, conveniently carry out high temperature cleaning work.
The pump 43 adopts a gas-liquid dual-purpose pump and a mixing pump, the tray 5 is positioned in the culture cabin, the middle part of the tray 5 is provided with a partition type bulge, the middle parts of the left side and the right side of the tray 5 are penetrated by the circulating pipe 44, and when the independent culture is needed, sealing covers can be covered on the two sides of the tray 5 to prevent cross infection. The inner wall of basin 41 separates into gas storehouse and liquid storehouse through fixed baffle 42, the bottom fixed intercommunication in liquid storehouse has injection valve 45, conveniently supply water to the liquid storehouse is inside, circulating pipe 44 encircles to cultivateing the inside in cabin, can increase the contact surface with the air, guarantee the heating effect, and inside the draw-in groove that the roof was seted up in cultivateing the cabin in top joint, the port intercommunication in circulating pipe 44 left side has first three-way pipe 46, the port on circulating pipe 44 right side communicates with the output of pump 43, the input intercommunication of pump 43 has second three-way pipe 47 (when the gas-liquid joint of pump 43 is the separate separation formula, can communicate respectively through the hose with the both ends of second three-way pipe 47 respectively), two reposition of redundant personnel ends of first three-way pipe 46 and second three-way pipe 47 are located the inside in gas storehouse and liquid storehouse respectively. The inner ring of the circulation pipe 44 is fixedly inlaid with symmetrical communication valves 91, two communication valves 91 positioned at the same horizontal position are communicated through an adding pipe 92, spray heads 93 distributed at equal distances are fixedly communicated at the bottom end of the adding pipe 92, sealing mechanisms such as air nozzles are arranged at the communication ends of the spray heads 93 and the adding pipe 92, when the power of the pump 43 is increased, the internal water flow pressure is increased, and the air nozzles can be opened for sprinkling cleaning work.
It should be noted that the structure of the present utility model may be implemented in many different forms, and is not limited to the embodiments, and any equivalent transformation made by those skilled in the art using the present specification and the accompanying drawings, or direct or indirect application in other related technical fields, such as loading and unloading of other articles, are included in the protection scope of the present utility model.
Claims (7)
1. Cell culture apparatus for cell engineering, including the box, its characterized in that: the inside and outside sealing mechanism that is used for sealed is equipped with to box department, the inside of box is fixed with a plurality of tray that is used for placing the culture dish, the ultraviolet lamp group that is used for disinfecting is installed to the inner wall of box, the internally mounted of box has the gas-liquid circulation mechanism that is used for pasteurization.
2. A cell culture apparatus for cell engineering according to claim 1, wherein: the sealing mechanism comprises an outer sealing door and an inner sealing door, the outer sealing door is hinged to the opening of the box body through a hinge, a sealing gasket is fixed on the outer ring of the outer sealing door, the inner sealing door comprises two door bodies hinged to the inside of the box body through a pin shaft and seals the inside of the box body into a culture cabin, motors which are driven synchronously and at the same speed are respectively arranged on the two door bodies, and a control switch for controlling the motors is arranged at the bottom of the box body.
3. A cell culture apparatus for cell engineering according to claim 2, wherein: the ultraviolet lamp group comprises a first ultraviolet lamp group and a second ultraviolet lamp group, the first ultraviolet lamp group is arranged on the inner bottom wall and the inner top wall of the box body and is positioned between the outer sealing door and the inner sealing door, and the second ultraviolet lamp group is arranged on the inner wall of the culture cabin.
4. A cell culture apparatus for cell engineering according to claim 2, wherein: the gas-liquid circulation mechanism comprises a water tank arranged on the inner bottom wall of the culture cabin, a pump arranged at the end angle of the culture cabin and a circulating pipe used for circulation, wherein the bottom end of the water tank is provided with a waterproof electric heating pipe, the inner wall of the water tank is divided into a gas cabin and a liquid cabin through a fixed partition plate, the bottom end of the liquid cabin is fixedly communicated with an injection valve, the circulating pipe surrounds the inside of the culture cabin, the top end of the circulating pipe is clamped inside a clamping groove formed in the inner top wall of the culture cabin, a port on the left side of the circulating pipe is communicated with a first three-way pipe, a port on the right side of the circulating pipe is communicated with the output end of the pump, the input end of the pump is communicated with a second three-way pipe, and two split ends of the first three-way pipe and the second three-way pipe are respectively positioned inside the gas cabin and the liquid cabin.
5. The cell culture apparatus for cell engineering according to claim 4, wherein: the inner ring of the circulating pipe is fixedly embedded with symmetrical communicating valves, two communicating valves positioned at the same horizontal position are communicated through an adding pipe, and spray heads distributed at equal distances are fixedly communicated at the bottom end of the adding pipe.
6. The cell culture apparatus for cell engineering according to claim 4, wherein: the tray is located inside the culture cabin, the middle part of the tray is provided with partition type bulges, and the middle parts of the left side and the right side of the tray are penetrated by the circulating pipe.
7. A cell culture apparatus for cell engineering according to claim 1, wherein: the inner wall of the box body is inlaid with an insulating layer.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321736205.7U CN220183296U (en) | 2023-07-04 | 2023-07-04 | Cell culture device for cell engineering |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321736205.7U CN220183296U (en) | 2023-07-04 | 2023-07-04 | Cell culture device for cell engineering |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220183296U true CN220183296U (en) | 2023-12-15 |
Family
ID=89102805
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321736205.7U Active CN220183296U (en) | 2023-07-04 | 2023-07-04 | Cell culture device for cell engineering |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220183296U (en) |
-
2023
- 2023-07-04 CN CN202321736205.7U patent/CN220183296U/en active Active
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110628603A (en) | A incubator for microbial inoculum | |
| KR102230047B1 (en) | Aseptic inoculation culture apparatus and aseptic method therefor | |
| CN1219049C (en) | Bacteria-free isolated operation system | |
| CN111254073B (en) | Constant temperature type bacterial strain culture device for preventing and treating infectious diseases special for veterinarian | |
| CN220183296U (en) | Cell culture device for cell engineering | |
| CN210044515U (en) | Medical apparatus is with degassing unit | |
| CN216366003U (en) | High-pressure steam sterilization cabinet | |
| CN212522491U (en) | Elevator degassing unit | |
| CN212940634U (en) | Disinfection cabinet for medical operation | |
| CN207238054U (en) | Microbial sterility operation console | |
| CN203842070U (en) | Disinfection cabinet | |
| CN207616624U (en) | Isolating device | |
| CN104513793A (en) | Aerobic solid-state fermentation system | |
| CN211658826U (en) | Culture dish bacteria removing device for genetic engineering experiments | |
| CN217516954U (en) | Automatic sealed shaking table case that disinfects | |
| CN215022769U (en) | Disinfection room for epidemic prevention and control | |
| CN215593083U (en) | Carbon dioxide cell culture case with water supply function | |
| CN218146721U (en) | Temperature-controllable liquid strain culture device | |
| CN220572432U (en) | Ethylene oxide sterilizer | |
| CN216712073U (en) | Electric heating incubator with gas circulation sterilization structure | |
| CN219489977U (en) | Manual mixed inoculation culture medium inoculation equipment | |
| CN214853437U (en) | Whiteflower hogfennel root callus induction device | |
| CN216536284U (en) | Instrument disinfection cabinet for anesthesia department | |
| CN216652924U (en) | Convenient microbial cultivation ware degassing unit | |
| CN216998414U (en) | Medical treatment is with microbial detection and quarantine culture apparatus |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant |