CN115053771A - Identification method for rice grain sterility caused by high temperature - Google Patents

Identification method for rice grain sterility caused by high temperature Download PDF

Info

Publication number
CN115053771A
CN115053771A CN202210663102.6A CN202210663102A CN115053771A CN 115053771 A CN115053771 A CN 115053771A CN 202210663102 A CN202210663102 A CN 202210663102A CN 115053771 A CN115053771 A CN 115053771A
Authority
CN
China
Prior art keywords
rice
temperature
seeds
sterility
identifying
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202210663102.6A
Other languages
Chinese (zh)
Inventor
谢鸿光
张建福
潘丽燕
魏林艳
魏毅东
罗曦
蔡秋华
王颖姮
何炜
郑燕梅
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Rice Research Institute Fujian Academy Of Agricultural Sciences
Original Assignee
Rice Research Institute Fujian Academy Of Agricultural Sciences
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Rice Research Institute Fujian Academy Of Agricultural Sciences filed Critical Rice Research Institute Fujian Academy Of Agricultural Sciences
Priority to CN202210663102.6A priority Critical patent/CN115053771A/en
Publication of CN115053771A publication Critical patent/CN115053771A/en
Pending legal-status Critical Current

Links

Images

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00—Cultivation of specific crops or plants not otherwise provided for
    • A01G22/20—Cereals
    • A01G22/22—Rice
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01C—PLANTING; SOWING; FERTILISING
    • A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01C—PLANTING; SOWING; FERTILISING
    • A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • A01C1/02—Germinating apparatus; Determining germination capacity of seeds or the like
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00—Botany in general
    • A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
    • A01G9/14—Greenhouses
    • A01G9/16—Dismountable or portable greenhouses ; Greenhouses with sliding roofs
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
    • A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
    • A01N63/22—Bacillus
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
    • A01N63/38—Trichoderma
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/03—Algae
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P21/00—Plant growth regulators

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Environmental Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Dentistry (AREA)
  • Agronomy & Crop Science (AREA)
  • Biotechnology (AREA)
  • Pest Control & Pesticides (AREA)
  • Mycology (AREA)
  • Virology (AREA)
  • Botany (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Ecology (AREA)
  • Forests & Forestry (AREA)
  • Soil Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Physiology (AREA)
  • Pretreatment Of Seeds And Plants (AREA)

Abstract

The invention belongs to the technical field of rice cultivation, and discloses an identification method for rice seed sterility caused by high temperature, which comprises the steps of soaking rice seeds in clear water added with a seed soaking agent, ventilating, and controlling the seed soaking and germination accelerating temperature; taking out and drying the rice seeds after the rice seeds fully absorb water and expose white, and raising the rice seedlings in a greenhouse by adopting a seedling tray; dry seedling raising is adopted, and the rice seedlings are transplanted into a special high-temperature planting pot when the rice reaches the seedling age; artificially removing tillers at the tillering end, moving potted rice into an artificial climate box for high-temperature treatment at the flowering period, and recording the flowering spike length in the high-temperature treatment period; and (3) putting the rice seedlings subjected to the high-temperature treatment into an artificial climate box with a contrast temperature, calculating the high-temperature relative maturing rate of the rice by taking the high-temperature relative maturing rate as a main identification and classification index, and identifying the sterility of the rice seeds caused by the high temperature. The method utilizes reasonable identification facilities and low cost, and can provide application support for the layout of high-temperature resistant varieties of rice and the obtainment of stable rice yield.

Description

Identification method for rice grain sterility caused by high temperature
Technical Field
The invention belongs to the technical field of rice cultivation, and particularly relates to an identification method for rice grain sterility caused by high temperature.
Background
At present, rice is a main food crop in China, more than 60% of people in China use rice as staple food, and the rice has very important significance in stabilizing the planting area and yield of the rice. The total yield of the rice is increased, and the living standard of people in China is improved. The rice planting area in China is wide, and the planting seasons are various. Global warming causes high temperature frequently in crop production seasons, rice flowering period and high temperature season meet due to addition of rice planting systems and modes, breeding technology progresses, crop varieties are multiple in types and quick to update, high temperature resistance of the crop varieties is not clear, high temperature damage causes yield reduction of food crops in China, and the high temperature damage becomes one of main factors which seriously restrict high and stable yield of the food crops in China. The influence of abnormal climate such as extreme high temperature causes the stability of rice production to be reduced, the risk of grain yield increase is increased, and the increase of the income of farmers and the social stability of rural areas are directly influenced.
However, the existing high-temperature-resistant identification method for rice varieties has the disadvantages that the utilized identification facilities are not matched, the standard identification temperature index is difficult to set, and the identification cost is high. Therefore, a new and reasonable high-temperature resistant identification method for rice varieties is needed to be constructed.
Through the above analysis, the problems and defects of the prior art are as follows: the existing high-temperature resistant identification method for rice varieties has the defects that identification facilities are not matched, the standard identification temperature index is difficult to set, and the identification cost is high.
Disclosure of Invention
Aiming at the problems in the prior art, the invention provides an identification method for rice grain sterility caused by high temperature.
The invention is realized in such a way that the identification method for the rice grain sterility caused by the high temperature comprises the following steps:
step one, pretreating rice seeds by ventilating, soaking and accelerating germination: soaking the rice seeds in clear water added with a seed soaking agent, ventilating, and controlling the seed soaking and germination accelerating temperature until the rice seeds are white after being broken; taking out and drying the rice seeds after the rice seeds fully absorb water and expose white, and raising the rice seedlings in a greenhouse by adopting a seedling tray;
step two, performing high-temperature treatment on rice plants: dry seedling raising is adopted, and the rice seedlings are transplanted into a special high-temperature planting pot when the rice reaches the seedling age; manually removing tillers at the last stage of tillering, transferring potted rice into an artificial climate box for high-temperature treatment at the flowering stage, and recording the length of blossoming ears in the high-temperature treatment stage;
step three, identifying the high-temperature resistant rice variety: and (3) putting the rice seedlings subjected to the high-temperature treatment into an artificial climate box with a contrast temperature, calculating the high-temperature relative maturing rate of the rice by taking the high-temperature relative maturing rate as a main identification and classification index, and identifying the sterility of the rice seeds caused by the high temperature.
Further, the pretreatment method for rice seed aeration seed soaking and germination acceleration in the first step comprises the following steps:
(1) soaking the rice seeds in clear water added with a seed soaking agent for 24-48 h, and ventilating the soaked rice seeds for 4-8 min at intervals of 8-12 min; wherein, the ventilation is to ventilate the bottom of the rice seeds by using a ventilation device which is an air pump;
(2) controlling the temperature to be 25-27 ℃ 24-36 h before the rice seed soaking and germination accelerating process, soaking the rice seeds under the constant temperature condition that the temperature is adjusted to be 32-35 ℃ for 24-36 h until the rice seeds are broken and white, and accelerating germination under the condition that the temperature is adjusted to be 26-27 ℃ for 12-24 h;
(3) after the rice seeds fully absorb the water and are exposed to be white, taking out the rice seeds and placing the rice seeds in a drying device for drying until no obvious water exists on the surfaces of the rice seeds; adopts a seedling tray to sprout and raise seedlings, and the seedling tray is placed in a greenhouse to stack seedlings to sprout and raise seedlings.
Further, the seed soaking agent comprises, by mass, 20-40 parts of procyanidine, 15-20 parts of plant growth regulator, 12-16 parts of microbial agent, 10-15 parts of sucrose, 8-12 parts of seaweed meal, 8-10 parts of chitin, 3-6 parts of insecticide and the balance of sterilized deionized water;
the seed soaking concentration of the procyanidine is 0.2-1.2%, and the seed soaking concentration of the sucrose is 0.2-0.4%;
the microbial agent is selected from any one or more of bacillus subtilis, bacillus licheniformis, trichoderma, bacillus laterosporus or arbuscular mycorrhizal agents;
the plant growth regulator is selected from one or more of brassinolide, ethylene, cytokinin, uniconazole, brassinolide or naphthylacetic acid;
the pesticide is any one or more of imidacloprid, dimehypo, dimethoate, fenpropathrin, acephate, carbosulfan or monocrotophos.
Further, the method for treating rice plants at high temperature in the second step comprises:
(1) raising rice seedlings in a dry way, lifting the rice seedlings when the rice seedlings reach the seedling age, and transplanting the rice seedlings into a special high-temperature planting pot; the seedling age of the rice during seedling raising is 25-30 days;
(2) manually removing at the middle and later tillers, keeping 6 big tillers with the same size in each cluster, and listing to record single ears, ears of rice to be treated at high temperature and contrast ears of rice;
(3) in the flowering period, transferring potted rice into an artificial climate box for high-temperature treatment for 2-4 days; after the high-temperature treatment, the length of the ear bloomed during the high-temperature treatment period was measured from the top of the ear downward, and a control group was set.
Further, the identification method of the high-temperature resistant rice variety in the third step comprises the following steps:
(1) putting the rice seedlings subjected to the high-temperature treatment into an artificial climate box with a contrast temperature, and recording the average fructification rate of glume flowers blooming in a single ear under the contrast temperature of the artificial climate box;
(2) and calculating the high-temperature relative maturing rate of the rice by using the average maturing rate of the tabbed ears blooming during the high-temperature treatment of the artificial climate box and the average maturing rate of the glumes blooming at the single ear under the contrast temperature of the artificial climate box and taking the high-temperature relative maturing rate as a main identification and grading index.
Further, the calculation method of the high-temperature relative maturing rate of the rice comprises the following steps:
taking the average glume-flower maturing rate of the hanging spike blossoming in the high-temperature treatment period of the artificial climate box as the high-temperature maturing rate, taking the average glume-flower maturing rate of the single spike blossoming in the artificial climate box as the contrast maturing rate, and calculating the high-temperature relative maturing rate of the rice by using the following formula:
high temperature relative setting percentage is high temperature setting percentage/contrast setting percentage x 100%.
The invention also aims to provide an identification system for the rice grain sterility caused by the high temperature, which applies the identification method for the rice grain sterility caused by the high temperature, and the identification system for the rice grain sterility caused by the high temperature comprises the following steps:
the seed soaking and germination accelerating module is connected with the central control module and is used for soaking the rice seeds in clear water added with a seed soaking agent and ventilating the bottoms of the rice seeds by using a ventilating device;
the seed drying treatment module is connected with the central control module, and is taken out after the rice seeds fully absorb water and are exposed to be white, and the rice seeds are placed in a drying device to be dried until no obvious water exists on the surfaces of the rice seeds;
the central control module is connected with the seed soaking and sprouting module, the seed drying treatment module, the seedling high-temperature treatment module and the high-temperature resistant variety identification module and is used for coordinating and controlling the normal operation of each module of the identification system for causing the rice seed sterility by the high temperature through the central processing unit;
the rice seedling high-temperature processing module is connected with the central control module, and is used for lifting and transplanting rice to a special high-temperature planting pot when the rice reaches the seedling age, and transferring potted rice into an artificial climate box for high-temperature processing in the flowering period;
and the high-temperature-resistant variety identification module is connected with the central control module and is used for calculating the high-temperature relative maturing rate of the rice by utilizing the average glume maturing rate of the hanging ears blooming during the high-temperature treatment of the artificial climate box and the average glume maturing rate of the single ears blooming at the contrast temperature of the artificial climate box.
Another objective of the present invention is to provide a computer device, which includes a memory and a processor, wherein the memory stores a computer program, and when the computer program is executed by the processor, the processor executes the method for identifying sterility of rice grain caused by high temperature.
Another objective of the present invention is to provide a computer-readable storage medium, which stores a computer program, wherein when the computer program is executed by a processor, the processor executes the method for identifying sterility of rice grain caused by high temperature.
The invention also aims to provide an information data processing terminal, which is used for realizing the identification system for rice grain sterility caused by high temperature.
In combination with the technical solutions and the technical problems to be solved, please analyze the advantages and positive effects of the technical solutions to be protected in the present invention from the following aspects:
first, aiming at the technical problems existing in the prior art and the difficulty in solving the problems, the technical problems to be solved by the technical scheme of the present invention are closely combined with the technical scheme to be protected and the results and data in the research and development process, and some creative technical effects brought after the problems are solved are analyzed in detail and deeply. The specific description is as follows:
according to the method for identifying the rice seed sterility caused by the high temperature, the rice seed soaking agent is used for pretreating rice seeds by ventilating, soaking and accelerating germination, so that the strong seedling rate and the tillering number can be improved, and the damage of various seed-borne diseases and soil-borne diseases can be resisted; the artificial climate box is used for carrying out high-temperature treatment on rice seedlings, the temperature change of the treatment is similar to that of the natural environment, the accuracy of the identification result is improved, and meanwhile, a method for variety identification and heat damage evaluation is provided for dealing with the high-temperature heat damage of the rice caused by climate warming.
Secondly, considering the technical scheme as a whole or from the perspective of products, the technical effect and advantages of the technical scheme to be protected by the invention are specifically described as follows:
the method for identifying the rice kernel sterility caused by the high temperature has the advantages of reasonable identification facilities and low cost, and provides application support for the layout of high-temperature resistant varieties of rice and the obtainment of stable rice yield.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings required to be used in the embodiments of the present invention will be briefly described below, and it is obvious that the drawings described below are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a flow chart of a method for identifying rice grain sterility caused by high temperature according to an embodiment of the present invention;
FIG. 2 is a flow chart of a pretreatment method for accelerating germination of rice seeds by aeration and seed soaking according to an embodiment of the present invention;
FIG. 3 is a flow chart of a method for hyperthermic treatment of rice plants according to an embodiment of the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
Aiming at the problems in the prior art, the invention provides a method for identifying rice kernel sterility caused by high temperature, and the invention is described in detail with reference to the accompanying drawings.
First, an embodiment is explained. This section is an explanatory embodiment expanding on the claims so as to fully understand how the present invention is embodied by those skilled in the art.
As shown in fig. 1, the identification method for rice grain sterility caused by high temperature provided by the embodiment of the invention comprises the following steps:
s101, pretreating rice seeds by ventilating, soaking and accelerating germination: soaking the rice seeds in clear water added with a seed soaking agent, ventilating, and controlling the seed soaking and germination accelerating temperature until the rice seeds are white after being broken; taking out and drying the rice seeds after the rice seeds fully absorb water and expose white, and raising the rice seedlings in a greenhouse by adopting a seedling tray;
s102, performing high-temperature treatment on rice plants: dry seedling raising is adopted, and the rice seedlings are transplanted into a special high-temperature planting pot when the rice reaches the seedling age; manually removing tillers at the last stage of tillering, transferring potted rice into an artificial climate box for high-temperature treatment at the flowering stage, and recording the length of blossoming ears in the high-temperature treatment stage;
s103, identifying the high-temperature resistant rice variety: and (3) putting the rice seedlings subjected to the high-temperature treatment into an artificial climate box with a contrast temperature, calculating the high-temperature relative maturing rate of the rice by taking the high-temperature relative maturing rate as a main identification and classification index, and identifying the sterility of the rice seeds caused by the high temperature.
As shown in fig. 2, the method for pre-treating rice seeds by aeration, seed soaking and germination accelerating in step S101 according to the embodiment of the present invention includes:
s201, soaking rice seeds in clear water added with a seed soaking agent for 24-48 hours, and ventilating the rice seeds soaked in the clear water for 4-8 minutes at intervals of 8-12 minutes; wherein, the ventilation is to ventilate the bottom of the rice seeds by using a ventilation device which is an air pump;
s202, controlling the temperature to be 25-27 ℃ 24-36 h before the rice seed soaking and sprouting process, soaking the rice seeds under the constant temperature condition that the temperature is adjusted to be 32-35 ℃ for 24-36 h until the rice seeds break the breast and are exposed to the white, and sprouting under the condition that the temperature is adjusted to be 26-27 ℃ for 12-24 h;
s203, after the rice seeds fully absorb the water and white, taking out the rice seeds and placing the rice seeds in a drying device for drying until no obvious water exists on the surfaces of the rice seeds; adopts a seedling tray to sprout and raise seedlings, and the seedling tray is placed in a greenhouse to stack seedlings to sprout and raise seedlings.
The seed soaking agent provided by the embodiment of the invention comprises, by mass, 20-40 parts of procyanidine, 15-20 parts of plant growth regulator, 12-16 parts of microbial agent, 10-15 parts of sucrose, 8-12 parts of seaweed meal, 8-10 parts of chitin, 3-6 parts of insecticide and the balance of sterilized deionized water;
wherein the seed soaking concentration of procyanidine is 0.2-1.2%, and the seed soaking concentration of sucrose is 0.2-0.4%;
the microbial agent is any one or more of bacillus subtilis, bacillus licheniformis, trichoderma, bacillus laterosporus and arbuscular mycorrhizal agent;
the plant growth regulator is selected from one or more of brassinolide, ethylene, cytokinin, uniconazole, brassinolide or naphthylacetic acid;
the pesticide is one or several of imidacloprid, dimehypo, dimethoate, fenpropathrin, acephate, carbosulfan and monocrotophos.
As shown in fig. 3, the method for high-temperature treatment of rice plants in step S102 according to the embodiment of the present invention includes:
s301, raising seedlings in a dry way, lifting the seedlings when the seedlings reach the seedling age, and transplanting the seedlings into a special high-temperature planting pot; the seedling age of the rice during seedling raising is 25-30 days;
s302, manually removing at the middle and later tillering stages, keeping 6 big tillers with the same size in each cluster, and listing and recording single ears, ears to be treated at high temperature and contrast ears;
s303, in the flowering period, transferring the potted rice into an artificial climate box for high-temperature treatment for 2-4 days; after the high-temperature treatment, the length of the ear bloomed during the high-temperature treatment period was measured from the top of the ear downward, and a control group was set.
The method for identifying the high-temperature resistant rice variety in the step S103 provided by the embodiment of the invention comprises the following steps:
(1) placing the rice seedlings subjected to high-temperature treatment into an artificial climate box with a reference temperature, and recording the average fructification rate of glume flowers blooming in a single spike under the reference temperature of the artificial climate box;
(2) and calculating the high-temperature relative maturing rate of the rice by using the average maturing rate of the tabbed ears blooming during the high-temperature treatment of the artificial climate box and the average maturing rate of the glumes blooming at the single ear under the contrast temperature of the artificial climate box and taking the high-temperature relative maturing rate as a main identification and grading index.
The method for calculating the high-temperature relative maturing rate of the rice provided by the embodiment of the invention comprises the following steps:
taking the average glume-flower maturing rate of the hanging spike blossoming in the high-temperature treatment period of the artificial climate box as the high-temperature maturing rate, taking the average glume-flower maturing rate of the single spike blossoming in the artificial climate box as the contrast maturing rate, and calculating the high-temperature relative maturing rate of the rice by using the following formula:
high temperature relative setting percentage is high temperature setting percentage/contrast setting percentage x 100%.
The identification system for rice grain sterility caused by high temperature provided by the embodiment of the invention comprises:
the seed soaking and germination accelerating module is connected with the central control module and is used for soaking the rice seeds in clear water added with a seed soaking agent and ventilating the bottoms of the rice seeds by using a ventilating device;
the seed drying treatment module is connected with the central control module, and is taken out after the rice seeds fully absorb water and are exposed to be white, and the rice seeds are placed in a drying device to be dried until no obvious water exists on the surfaces of the rice seeds;
the central control module is connected with the seed soaking and sprouting module, the seed drying treatment module, the seedling high-temperature treatment module and the high-temperature resistant variety identification module and is used for coordinating and controlling the normal operation of each module of the identification system for causing the rice seed sterility by the high temperature through the central processing unit;
the rice seedling high-temperature processing module is connected with the central control module, and is used for lifting and transplanting rice to a special high-temperature planting pot when the rice reaches the seedling age, and transferring potted rice into an artificial climate box for high-temperature processing in the flowering period;
and the high-temperature-resistant variety identification module is connected with the central control module and is used for calculating the high-temperature relative maturing rate of the rice by utilizing the average glume maturing rate of the hanging ears blooming during the high-temperature treatment of the artificial climate box and the average glume maturing rate of the single ears blooming at the contrast temperature of the artificial climate box.
And II, application embodiment. In order to prove the creativity and the technical value of the technical scheme of the invention, the part is an application example of the technical scheme of the claims to a specific product or related technology.
The application embodiment of the invention provides computer equipment, which comprises a memory and a processor, wherein the memory stores a computer program, and when the computer program is executed by the processor, the processor executes the identification method for rice grain sterility caused by high temperature.
The application embodiment of the invention provides a computer-readable storage medium, which stores a computer program, and when the computer program is executed by a processor, the processor executes the identification method for rice grain sterility caused by high temperature.
The application embodiment of the invention provides an information data processing terminal, which is used for realizing the identification system for rice grain sterility caused by high temperature.
It should be noted that embodiments of the present invention can be realized in hardware, software, or a combination of software and hardware. The hardware portion may be implemented using dedicated logic; the software portions may be stored in a memory and executed by a suitable instruction execution system, such as a microprocessor or specially designed hardware. Those skilled in the art will appreciate that the apparatus and methods described above may be implemented using computer executable instructions and/or embodied in processor control code, such code being provided on a carrier medium such as a disk, CD-or DVD-ROM, programmable memory such as read only memory (firmware), or a data carrier such as an optical or electronic signal carrier, for example. The apparatus and its modules of the present invention may be implemented by hardware circuits such as very large scale integrated circuits or gate arrays, semiconductors such as logic chips, transistors, or programmable hardware devices such as field programmable gate arrays, programmable logic devices, etc., or by software executed by various types of processors, or by a combination of hardware circuits and software, e.g., firmware.
The above description is only for the purpose of illustrating the present invention and the appended claims are not to be construed as limiting the scope of the invention, which is intended to cover all modifications, equivalents and improvements that are within the spirit and scope of the invention as defined by the appended claims.

Claims (10)

1. The method for identifying the rice kernel sterility caused by the high temperature is characterized by comprising the following steps of:
step one, pretreating rice seeds by ventilating, soaking and accelerating germination: soaking the rice seeds in clear water added with a seed soaking agent, ventilating, and controlling the seed soaking and germination accelerating temperature until the rice seeds are white after being broken; taking out and drying after the rice seeds fully absorb water and reveal white, and raising seedlings by adopting a seedling tray in a greenhouse;
step two, performing high-temperature treatment on rice plants: dry seedling raising is adopted, and the rice seedlings are transplanted into a special high-temperature planting pot when the rice reaches the seedling age; manually removing tillers at the last stage of tillering, transferring potted rice into an artificial climate box for high-temperature treatment at the flowering stage, and recording the length of blossoming ears in the high-temperature treatment stage;
step three, identifying the high-temperature resistant rice variety: and (3) putting the rice seedlings subjected to the high-temperature treatment into an artificial climate box with a contrast temperature, calculating the high-temperature relative maturing rate of the rice by taking the high-temperature relative maturing rate as a main identification and classification index, and identifying the sterility of the rice seeds caused by the high temperature.
2. The method for identifying rice kernel sterility due to high temperature as claimed in claim 1, wherein the pretreatment method for rice seed aeration seed soaking pregermination in the first step comprises the following steps:
(1) soaking the rice seeds in clear water added with a seed soaking agent for 24-48 h, and ventilating the soaked rice seeds for 4-8 min at intervals of 8-12 min; wherein, the ventilation is to ventilate the bottom of the rice seeds by using a ventilation device which is an air pump;
(2) controlling the temperature to be 25-27 ℃ 24-36 h before the rice seed soaking and germination accelerating process, soaking the rice seeds under the constant temperature condition that the temperature is adjusted to be 32-35 ℃ for 24-36 h until the rice seeds are broken and white, and accelerating germination under the condition that the temperature is adjusted to be 26-27 ℃ for 12-24 h;
(3) after the rice seeds fully absorb the water and are exposed to be white, taking out the rice seeds and placing the rice seeds in a drying device for drying until no obvious water exists on the surfaces of the rice seeds; adopts a seedling tray to sprout and raise seedlings, and the seedling tray is placed in a greenhouse to stack seedlings to sprout and raise seedlings.
3. The method for identifying the sterility of rice grains caused by the high temperature as claimed in claim 2, wherein the seed soaking agent comprises, by mass, 20-40 parts of procyanidine, 15-20 parts of plant growth regulator, 12-16 parts of microbial agent, 10-15 parts of sucrose, 8-12 parts of seaweed meal, 8-10 parts of chitin, 3-6 parts of insecticide and the balance of sterilized deionized water;
the seed soaking concentration of the procyanidine is 0.2-1.2%, and the seed soaking concentration of the sucrose is 0.2-0.4%;
the microbial agent is any one or a combination of bacillus subtilis, bacillus licheniformis, trichoderma, bacillus laterosporus or arbuscular mycorrhizal agent;
the plant growth regulator is selected from one or more of brassinolide, ethylene, cytokinin, uniconazole, brassinolide or naphthylacetic acid;
the pesticide is any one or more of imidacloprid, dimehypo, dimethoate, fenpropathrin, acephate, carbosulfan or monocrotophos.
4. The method for identifying rice kernel sterility due to high temperature of claim 1, wherein the method for treating rice plants with high temperature in the second step comprises:
(1) raising rice seedlings in a dry way, lifting the rice seedlings when the rice seedlings reach the seedling age, and transplanting the rice seedlings into a special high-temperature planting pot; the seedling age of the rice during seedling raising is 25-30 days;
(2) manually removing at the middle and later tillers, keeping 6 big tillers with the same size in each cluster, and listing to record single ears, ears of rice to be treated at high temperature and contrast ears of rice;
(3) in the flowering period, transferring potted rice into an artificial climate box for high-temperature treatment for 2-4 days; after the high-temperature treatment, the length of the ear bloomed during the high-temperature treatment period was measured from the top of the ear downward, and a control group was set.
5. The method for identifying rice kernel sterility due to high temperature as claimed in claim 1, wherein the method for identifying the high temperature resistant rice variety in step three comprises:
(1) putting the rice seedlings subjected to the high-temperature treatment into an artificial climate box with a contrast temperature, and recording the average fructification rate of glume flowers blooming in a single ear under the contrast temperature of the artificial climate box;
(2) and calculating the high-temperature relative maturing rate of the rice by using the average maturing rate of the tabbed ears blooming during the high-temperature treatment of the artificial climate box and the average maturing rate of the glumes blooming at the single ear under the contrast temperature of the artificial climate box and taking the high-temperature relative maturing rate as a main identification and grading index.
6. The method for identifying rice kernel sterility due to high temperature as claimed in claim 5, wherein the method for calculating the relative maturing rate of rice at high temperature comprises:
taking the average glume-flower maturing rate of the hanging spike blossoming in the high-temperature treatment period of the artificial climate box as the high-temperature maturing rate, taking the average glume-flower maturing rate of the single spike blossoming in the artificial climate box as the contrast maturing rate, and calculating the high-temperature relative maturing rate of the rice by using the following formula:
high temperature relative setting percentage is high temperature setting percentage/contrast setting percentage x 100%.
7. The system for identifying the rice grain sterility caused by the high temperature by applying the method for identifying the rice grain sterility caused by the high temperature according to any one of claims 1 to 6, is characterized by comprising the following steps:
the seed soaking and germination accelerating module is connected with the central control module and is used for soaking the rice seeds in clear water added with a seed soaking agent and ventilating the bottoms of the rice seeds by using a ventilating device;
the seed drying treatment module is connected with the central control module, and is taken out and placed in a drying device to be dried until no obvious moisture exists on the surfaces of the rice seeds after the rice seeds fully absorb the moisture and are exposed to be white;
the central control module is connected with the seed soaking and sprouting module, the seed drying treatment module, the seedling high-temperature treatment module and the high-temperature resistant variety identification module and is used for coordinating and controlling the normal operation of each module of the identification system for causing the rice seed sterility by the high temperature through the central processing unit;
the seedling high-temperature treatment module is connected with the central control module, and is used for lifting and transplanting rice to a special high-temperature planting pot when the rice reaches the seedling age, and transplanting potted rice into an artificial climate box for high-temperature treatment in the flowering period;
and the high-temperature-resistant variety identification module is connected with the central control module and is used for calculating the high-temperature relative maturing rate of the rice by utilizing the average glume maturing rate of the hanging ears blooming during the high-temperature treatment of the artificial climate box and the average glume maturing rate of the single ears blooming at the contrast temperature of the artificial climate box.
8. A computer device, comprising a memory and a processor, wherein the memory stores a computer program, and the computer program, when executed by the processor, causes the processor to execute the method for identifying sterility of rice grain caused by high temperature according to any one of claims 1-6.
9. A computer readable storage medium storing a computer program which, when executed by a processor, causes the processor to perform the method for identifying sterility of rice grain caused by high temperature according to any one of claims 1 to 6.
10. An information data processing terminal, wherein the information data processing terminal is used for realizing the identification system for rice grain sterility caused by high temperature according to claim 7.
CN202210663102.6A 2022-06-13 2022-06-13 Identification method for rice grain sterility caused by high temperature Pending CN115053771A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202210663102.6A CN115053771A (en) 2022-06-13 2022-06-13 Identification method for rice grain sterility caused by high temperature

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202210663102.6A CN115053771A (en) 2022-06-13 2022-06-13 Identification method for rice grain sterility caused by high temperature

Publications (1)

Publication Number Publication Date
CN115053771A true CN115053771A (en) 2022-09-16

Family

ID=83200717

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202210663102.6A Pending CN115053771A (en) 2022-06-13 2022-06-13 Identification method for rice grain sterility caused by high temperature

Country Status (1)

Country Link
CN (1) CN115053771A (en)

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102487771A (en) * 2011-12-28 2012-06-13 宁波市农业科学研究院 Heading stage high-temperature resistance identifying method for rice variety
CN103828666A (en) * 2014-03-07 2014-06-04 四川省农业科学院水稻高粱研究所 Filed identification method of flowering stage high temperature resistance of rice varieties
CN203840868U (en) * 2014-05-12 2014-09-24 宜春学院 System for authenticating high temperature resistance of paddy rice
CN105850622A (en) * 2016-04-28 2016-08-17 中国水稻研究所 Identification method for rice grain sterility caused by high temperatures
CN107047160A (en) * 2017-03-27 2017-08-18 湖南省水稻研究所 A kind of authentication method of high temperature resistant paddy rice and its application in breeding
US20220167571A1 (en) * 2020-11-27 2022-06-02 China National Rice Research Institute Method for Avoiding High Temperature and Maintaining Yield of Rice

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102487771A (en) * 2011-12-28 2012-06-13 宁波市农业科学研究院 Heading stage high-temperature resistance identifying method for rice variety
CN103828666A (en) * 2014-03-07 2014-06-04 四川省农业科学院水稻高粱研究所 Filed identification method of flowering stage high temperature resistance of rice varieties
CN203840868U (en) * 2014-05-12 2014-09-24 宜春学院 System for authenticating high temperature resistance of paddy rice
CN105850622A (en) * 2016-04-28 2016-08-17 中国水稻研究所 Identification method for rice grain sterility caused by high temperatures
CN107047160A (en) * 2017-03-27 2017-08-18 湖南省水稻研究所 A kind of authentication method of high temperature resistant paddy rice and its application in breeding
US20220167571A1 (en) * 2020-11-27 2022-06-02 China National Rice Research Institute Method for Avoiding High Temperature and Maintaining Yield of Rice

Similar Documents

Publication Publication Date Title
Ohnishi et al. Low temperature stress at different flower developmental stages affects pollen development, pollination, and pod set in soybean
May et al. Pre-sowing hardening of plants to drought: an appraisal of the contributions by PA Genkel.
CN106538395B (en) A method of improving endangered plants Tibet Paeonia ludlowii planting percent and seedling percent
Warrington et al. Corn Growth Response to Temperature and Photoperiod. III. Leaf Number 1
Gale et al. The induction of germination alpha-amylase during wheat grain development in unfavourable weather conditions
EP4021165A1 (en) Method and system for time of pollinating cereal crops
CN106941811A (en) A kind of method for shortening rice paddy seed rest period, promoting it to germinate
CN115053771A (en) Identification method for rice grain sterility caused by high temperature
Miedema et al. Photosynthesis and respiration of maize seedlings at suboptimal temperatures
CN110036908A (en) A method of accelerating corn inbred lines
Pei et al. Effects of high air temperature, drought, and both combinations on maize: A case study
CN110036716A (en) A kind of presoaking and germinating method of corn seed
CN109392583A (en) A method of promote peach embryo to train rosette-stape seedling seedling
CN101878733B (en) Method for promoting germination of guineagrass seed
Matsuda et al. Effect of temperatures on passion fruit flowering: a simulation model to estimate number of flowers
CN112544371B (en) Method for identifying low temperature resistance of maize inbred line in germination period
CN103636310B (en) Physical method urgees the preprocess method of bud to gingko seeds
da Silva et al. Sugarcane bud chip encapsulation for ex vitro synthetic seed formation
CN107326083B (en) Method for detecting purity of watermelon 'Jingmei' hybrid seed
CN105165174B (en) A kind of method for detecting wheat Growth habit and photoperiod characteristic
CN115226433B (en) Treatment method for improving germination of nopalina cochinchinensis seeds and promoting seedling growth
CN117344053B (en) Method for evaluating physiological development process of plant tissue
CN209643307U (en) A kind of device of high flux screening transgenic homozygous plant
Ionuț et al. Evaluation of photosynthesis capacity of some winter wheat genotypes in Transylvanian plain conditions
CN116210709B (en) Application of 5-O-methyl-vidamiloride in plant root system development

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination