CN115038791A - 重链抗体的重链可变区的制造方法 - Google Patents

重链抗体的重链可变区的制造方法 Download PDF

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CN115038791A
CN115038791A CN202080094749.0A CN202080094749A CN115038791A CN 115038791 A CN115038791 A CN 115038791A CN 202080094749 A CN202080094749 A CN 202080094749A CN 115038791 A CN115038791 A CN 115038791A
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西口大贵
和田真由美
东条卓人
井上英俊
影山泰
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Abstract

本发明提供一种以高产量制造重链抗体的重链可变区(VHH)的方法。该VHH的制造方法包括对芽孢杆菌属菌进行培养的步骤，该芽孢杆菌属菌导入有编码VHH的基因，并且缺损细胞外蛋白酶。

Description

重链抗体的重链可变区的制造方法
技术领域
本发明涉及一种重链抗体的重链可变区的制造方法。
背景技术
低分子抗体由于具有制造效率、药品开发设计的容易性、组织渗透性等优点，作为抗体医药和试剂的材料受到了关注。作为代表性的低分子抗体，可以列举Fab、单链抗体(single chain antibody；scFv)、双链抗体(diabody)、重链抗体的重链可变区(variabledomain of heavy chain of heavy chain antibody、VHH)等。骆驼科动物(双峰驼、单峰驼、大羊驼等)和鲨鱼等软骨鱼类的血清含有不具有轻链而只由重链构成的抗体(重链抗体；Heavy-chain antibody)。该重链抗体的可变区为VHH。VHH是能够利用单结构域识别抗原的分子，是迄今为止发现的抗体分子中的最小单位。另外，具有多个结构域或人源化结构域的医药用VHH也在销售。
目前，在抗体的微生物学生产中主要使用大肠杆菌。然而，在利用大肠杆菌生产异种蛋白质时，如果高分子量分子的生产率低，则有因内毒素而导致产物污染、因内毒素除去处理而引起产物的产量降低等问题。在非专利文献1中记载了，短芽孢杆菌能够克服上述的大肠杆菌的缺点，利用短芽孢杆菌表达系统，能够生产scFv或Fab、VHH。在专利文献1中记载了，有使用曲霉属等丝状菌制造VHH这样的报告。
枯草杆菌等芽孢杆菌属菌分泌AprE、Epr、WprA、Mpr、NprB、Bpr、NprE、Vpr、AprX等细胞外蛋白酶。在利用芽孢杆菌属菌的异种蛋白质生产中，已知这些细胞外蛋白酶是降低目标蛋白质的生产率的主要原因。在专利文献2中记载了利用缺损AprX等细胞外蛋白酶的芽孢杆菌属株，提高异种蛋白质的生产率。在非专利文献2中记载了，使细胞内和细胞外分子伴侣共表达并且使细胞壁结合型蛋白酶WprA钝化了的枯草杆菌株分泌生产来自纤维蛋白特异性单克隆抗体的scFv。另一方面，在非专利文献2中也记载了利用缺损7种细胞外蛋白酶的枯草杆菌株不能生产该scFv。
(专利文献1)日本特开2012-157315号公报
(专利文献2)日本特开2006-174707号公报
(非专利文献1)生物工程学,2017,95(11):649-651
(非专利文献2)Appl Environ Microbiol,2002,p.3261-3269
发明内容
本发明提供一种重链抗体的重链可变区的制造方法，其包括对芽孢杆菌属菌进行培养的步骤，该芽孢杆菌属菌导入有编码重链抗体的重链可变区的基因，并且缺损细胞外蛋白酶。
本发明还提供一种导入有编码重链抗体的重链可变区的基因并且缺损细胞外蛋白酶的芽孢杆菌属菌。
附图说明
图1是关于来自导入了VHH基因的细胞外蛋白酶单一缺损重组枯草杆菌株的培养上清液的SDS-PAGE像。箭头表示VHH蛋白质的条带位置。
图2是利用蛋白酶多重缺损株Dpr9ΔsigF的医药VHH分子的表达。
图3是Dpr9ΔsigF所表达的奥利组单抗(Ozoralizumab)和卡普赛珠单抗(Caplacizumab)的蛋白质印迹图像。﹡表示全长，＜表示分解产物。
图4是枯草杆菌表达VHH(1ZVH)的抗原(HEWL)结合活性。
图5是关于导入了VHH基因的大肠杆菌株的培养物的蛋白质印迹图像。
具体实施方式
在本说明书中，“抗体”是指通过来自免疫球蛋白的抗原识别区能够与蛋白质等靶标特异性结合的分子。
在本说明书中，“VHH”是指重链抗体的重链可变区(variable domain of heavychain of heavy chain antibody)。重链抗体在骆驼科动物、鲨鱼等软骨鱼类等中被发现。本说明书中的VHH例如来自骆驼科动物或鲨鱼，优选来自骆驼科动物。VHH是能够利用单结构域识别抗原的分子，是迄今为止发现的抗体分子中的最小单位。利用本发明制造的VHH可以含有1个以上的来自重链抗体的重链可变区，该VHH所含的重链可变区的个数没有限定。利用本发明制造的VHH所含的该重链可变区可以为来自天然的重链抗体的重链可变区，可以为其变体。利用本发明制造的VHH还可以包含来自该免疫球蛋白重链的重链可变区以外的片段(例如重链抗体的恒定区的片段、或来自人的片段、接头序列等)、用于稳定化的氨基酸变异等。利用本发明制造的VHH，作为抗原识别结构域，不具有来自免疫球蛋白轻链的可变区。例如，利用本发明制造的VHH不包括包含免疫球蛋白轻链可变区的单链抗体(例如scFv)。
在本说明书中，作为骆驼科动物，可以列举双峰驼、单峰驼、大羊驼、羊驼、骆马、原驼等，优选列举羊驼。
在本说明书中，核苷酸序列和氨基酸序列的同一性可以利用Lipman-Pearson法(Science,1985,227:1435-1441)进行计算。具体而言，可以利用遗传信息处理软件Genetyx-Win的同源性分析(Search homology)程序，将Unit size to compare(ktup)设为2进行分析而算出。
在本说明书中，关于氨基酸序列或核苷酸序列的“至少85％的同一性”是指85％以上、优选90％以上、更优选93％以上、进一步优选95％以上、进一步优选96％以上、进一步优选97％以上、进一步优选98％以上、进一步优选99％以上、进一步优选99.5％以上的同一性。
在本说明书中，调控区与基因的“可操作连接”是指将基因和调控区以该基因能够在该调控区的控制下表达的方式连结。基因和调控区的“可操作连接”的方法对于本领域技术人员是众所周知的。
在本说明书中，关于基因的“上游”和“下游”是指该基因的转录方向的上游和下游。例如，“配置在启动子下游的基因”是指在DNA有义链中在启动子的3’侧存在该基因，基因的上游是指DNA有义链中的该基因的5’侧的区域。
在本说明书中，细胞中的蛋白酶的“缺损”是指该细胞中的该蛋白酶的活性与该细胞原本(非缺损株、例如野生株)的活性相比下降了。蛋白酶的“缺损”不仅包含蛋白酶蛋白质的表达下降或丧失，而且包含蛋白酶蛋白质的功能下降或丧失。缺损蛋白酶的细胞(蛋白酶缺损株)可以通过细胞中的该蛋白酶基因的部分或全部缺失或钝化(所谓的敲落或敲除等)而制作。关于本说明书中的蛋白酶缺损株，该蛋白酶的活性相对于野生株优选下降至50％以下，更优选下降至25％以下。
在本说明书中，对于细胞的功能、性状、特质所使用的术语“内源”是为了表示该功能、性状、特质原本就存在于该细胞中。相对而言，使用术语“外源”是为了表示不是原本存在于该细胞而是从外部导入的功能、性状、特质。例如，“外源”基因或多核苷酸是从外部导入细胞内的基因或多核苷酸。外源基因或多核苷酸可以来自与导入其的细胞同种的生物，也可以来自异种的生物(即异种基因或多核苷酸)。
在本说明书中，“芽孢杆菌属菌”是指芽孢杆菌科(Bacillaceae)芽孢杆菌属(Bacillus)的菌。作为芽孢杆菌属菌的例子，可以列举枯草杆菌(Bacillus subtilis)、蜡样芽孢杆菌(Bacillus cereus)、苏云金芽孢杆菌(Bacillus thuringiensis)、巨大芽孢杆菌(Bacillus megaterium)、解淀粉芽孢杆菌(Bacillus amyloliquefaciens)、短小芽孢杆菌(Bacillus pumilus)、地衣芽孢杆菌(Bacillus licheniformis)等。其中，本说明书中的芽孢杆菌属菌不包括类芽孢杆菌科(Paenibacillaceae)所属的类芽孢杆菌属(Paenibacillus)或短芽孢杆菌属(Brevibacillus)的菌。
本说明书所记载的枯草杆菌的基因的名称基于日本枯草杆菌功能分析网络(JAFAN：Japan Functional Analysis Network for Bacillus subtilis)(BSORF DB)中网络公开([bacillus.genome.ad.jp/]、2006年1月18日更新)的枯草杆菌基因组数据进行记载。本说明书所记载的枯草杆菌的基因编号表示BSORF DB注册的基因编号。
本发明提供一种重链抗体的重链可变区(VHH)的制造方法。
本发明的发明人发现，利用缺损细胞外蛋白酶的芽孢杆菌属菌，能够以高产量制造VHH。
利用本发明，能够以高产量制造VHH。本发明能够克服现有的利用大肠杆菌的抗体生产的产量低或内毒素所导致的污染的问题。另外，芽孢杆菌属菌在酶等有用物质生产中的使用卓有成效，并且也无害，因此本发明的方法其操作容易并且安全。
本发明提供一种VHH的制造方法，其包括对芽孢杆菌属菌进行培养的步骤，该芽孢杆菌属菌导入有编码VHH的基因，并且缺损细胞外蛋白酶。
利用本发明制造的VHH如上述所规定的那样。作为利用本发明制造的VHH的更具体的例子，可以列举由序列号2、4、6、8、10、12、14、16、18、20、22、24、26、28、30、32、34、36和38的氨基酸序列构成的多肽。这些VHH的详细信息如表1所示。
[表1]
Figure BDA0003766872860000051
作为利用本发明制造的VHH的其它例子，可以列举由与序列号2、4、6、8、10、12、14、16、18、20、22、24、26、28、30、32、34、36和38的氨基酸序列中的任意种至少85％相同的氨基酸序列构成的多肽。
作为利用本发明制造的VHH的另外其它的例子，可以列举由序列号64、66、68、70、72、74、76、78、80、82、84、86、88、90、92、94、96、98、100、102、104、106、108、110、112、114、116、118、120、122、124、126、128、130、132、134、136和138的氨基酸序列构成的多肽。作为利用本发明制造的VHH的另外其它的例子，可以列举由与序列号64、66、68、70、72、74、76、78、80、82、84、86、88、90、92、94、96、98、100、102、104、106、108、110、112、114、116、118、120、122、124、126、128、130、132、134、136和138的氨基酸序列中的任意种至少85％相同的氨基酸序列构成的多肽。
前述的编码VHH的基因(多核苷酸)可以按照常规方法制备。作为该编码VHH的基因的例子，可以列举由序列号1、3、5、7、9、11、13、15、17、19、21、23、25、27、29、31、33、35、37、63、65、67、69、71、73、75、77、79、81、83、85、87、89、91、93、95、97、99、101、103、105、107、109、111、113、115、117、119、121、123、125、127、129、131、133、135和137的核苷酸序列构成的多核苷酸、以及与这些中的任意种至少85％序列相同的多核苷酸。例如，编码VHH的基因的多核苷酸可以基于序列信息进行化学合成。或者，编码VHH的基因的多核苷酸可以从骆驼科动物的基因组DNA中分离。对于所得到的基因，还可以进行突变导入。
该编码VHH的基因可以与调控区可操作地连接。在本说明书中，“调控区”是具有控制配置于其下游的基因的细胞内的表达的功能，优选具有组成上表达或高表达配置于该下游的基因的功能的区域。更具体而言，可以定义为存在于基因的编码区的上游并且具有RNA聚合酶相互作用而控制该编码区的转录的功能的区域。优选本说明书中的调控区是指基因的编码区的上游200～600核苷酸左右的区域。调控区包括转录起始调控区和/或翻译起始调控区、或者转录起始调控区至翻译起始调控区的区域。转录起始调控区是包含启动子和转录起始位点的区域，翻译起始调控区是相当于与起始密码子一起形成核糖体结合部位的Shine-Dalgarno(SD)序列的部位(Proc Natl Acad Sci USA,1974,71:1342-1346)。
作为该调控区的优选例，可以列举在芽孢杆菌属菌中发挥功能的调控区，例如来自芽孢杆菌属细菌的α-淀粉酶基因、蛋白酶基因、aprE基因或spoVG基因的调控区、芽孢杆菌KSM-S237株的纤维素酶基因(日本特开2000-210081号公报)的调控区、芽孢杆菌KSM-64株的纤维素酶基因(日本特开2011-10387公报)的调控区、以及来自金黄色葡萄球菌(Staphylococcus aureus)的卡那霉素抗性基因或氯霉素抗性基因的调控区(均参照日本特开2009-089708号公报)等，但没有特别限定。作为该调控区的更优选的例子，可以列举芽孢杆菌KSM-S237株的纤维素酶基因的启动子(序列号39)、芽孢杆菌KSM-64株的纤维素酶基因的启动子(序列号40)、枯草杆菌spoVG基因的启动子(序列号41)。另外，作为优选的调控区，可以列举与序列号39～41中的任意种具有至少85％的同一性并且具有控制基因的转录和翻译的功能的多核苷酸。
另外，该编码VHH的基因也可以与编码具有使所表达的蛋白质向细胞外分泌的功能的分泌信号的序列(称为分泌信号序列)可操作地连接。作为该分泌信号序列的优选例，可以列举在芽孢杆菌属菌中发挥功能的分泌信号序列，例如来自芽孢杆菌属菌的分泌信号序列。作为来自芽孢杆菌属菌的分泌信号序列的优选例，可以将芽孢杆菌KSM-S237株的纤维素酶基因的分泌信号序列(序列号42)、芽孢杆菌KSM-64株的纤维素酶基因的分泌信号序列(序列号43)、枯草杆菌淀粉酶基因amyE的分泌信号序列(序列号44)等。作为来自芽孢杆菌属菌的分泌信号序列的进一步的例子，可以列举与序列号42～44中的任意种具有至少85％的同一性并且具有使所表达的蛋白质向细胞外分泌的功能的多核苷酸。
该编码VHH的基因除了含有开放读码框(ORF)以外，还可以含有非翻译区域(UTR)的核苷酸序列。例如，该基因可以含有上述的启动子、分泌信号序列和终止子。
该基因向芽孢杆菌属菌的导入可以按照常规方法进行。例如可以将该基因或包含其的载体导入宿主芽孢杆菌属菌细胞内，再向该宿主细胞的基因组导入该基因。或者，也可以将包含该基因的表达载体导入宿主芽孢杆菌属菌细胞内。
基因或载体向宿主芽孢杆菌属菌细胞内的导入例如可以应用感受态细胞法、电穿孔法、原生质体法、粒子枪法、PEG法等公知的转化技术。
作为导入该编码VHH的基因的宿主芽孢杆菌属菌，可以列举前述的芽孢杆菌科芽孢杆菌属的菌，其中优选枯草杆菌或其突变株。
包含该编码VHH的基因的载体可以通过利用常规方法将该基因和根据需要含有调控区或分泌信号序列的多核苷酸插入任意的载体中并连接而构建。该载体的种类没有特别限定，可以为质粒、噬菌体、噬粒、黏粒、病毒、YAC载体、穿梭载体等任意的载体。另外，该载体优选为在宿主细胞内能够扩增的载体，更优选为表达载体。作为优选的载体的例子，没有限定，可以列举：pHA3040SP64、pHSP64R或pASP64(日本专利第3492935号)、pHY300PLK(能够转化大肠杆菌和枯草杆菌双方的表达载体；Jpn J Genet,1985,60：235-243)、pAC3(Nucleic Acids Res,1988,16：8732)等穿梭载体；pUB110(J Bacteriol,1978,134：318-329)、pTA10607(Plasmid,1987,18：8-15)等芽孢杆菌属细菌的转化能够利用的质粒等。还可以使用来自大肠杆菌的质粒(例如pET22b(+)、pBR322、pBR325、pUC57、pUC118、pUC119、pUC18、pUC19、pBluescript等)。
在本发明中，用于制造VHH的芽孢杆菌属菌除了导入了该编码VHH的基因以外，还缺损细胞外蛋白酶。该芽孢杆菌属菌中所缺损的细胞外蛋白酶优选为选自epr、wprA、bpr、vpr、aprE、aprX和与它们相当的基因所编码的细胞外蛋白酶中的至少1种，更优选为选自epr、wprA、bpr、vpr、aprE和apr所编码的蛋白酶中的至少1种，进一步优选为epr、wprA、bpr、vpr、aprE或apr所编码的蛋白酶，进一步优选为wprA或bpr所编码的蛋白酶。
epr、wprA、bpr、vpr、aprE和aprX均为枯草杆菌基因。将这些基因的基因编号和所编码的蛋白质的功能示于表2。作为与epr、wprA、bpr、vpr、aprE和aprX相当的基因，可以分别列举与epr、wprA、bpr、vpr、aprE和aprX在核苷酸序列中具有至少85％的同一性并且(记载在表2中)编码相同功能的蛋白质的来自芽孢杆菌属菌的基因。这些基因可以在上述的BSORF DB中检索。
[表2]
Figure BDA0003766872860000091
本发明所使用的芽孢杆菌属菌也可以缺损除前述的epr、wprA、bpr、vpr、aprE和aprX所编码的细胞外蛋白酶以外的其它细胞外蛋白酶。例如，本发明所使用的芽孢杆菌属菌可以缺损全部epr、wprA、mpr、nprB、bpr、nprE、vpr、aprE和aprX所编码的细胞外蛋白酶。本发明所使用的芽孢杆菌属菌还可以实施除细胞外蛋白酶缺损以外的其它改变，例如sigF等的σ因子缺失等。
缺损细胞外蛋白酶的芽孢杆菌属菌可以按照常规方法制造。例如通过缺失或钝化该菌的基因组DNA中编码细胞外蛋白酶的基因，能够制造缺损该细胞外蛋白酶的芽孢杆菌属菌。因此，在本发明的一个实施方式中，用于制造VHH的芽孢杆菌属菌缺失或钝化了选自epr或与其相当的基因、wprA或与其相当的基因、bpr或与其相当的基因、vpr或与其相当的基因、aprE或与其相当的基因和aprX或与其相当的基因中的至少1种基因。优选该芽孢杆菌属菌缺失或钝化了选自epr、wprA、bpr、vpr、aprE和aprX中的至少1种基因。更优选该芽孢杆菌属菌缺失或钝化了epr、wprA、bpr、vpr、aprE或aprX。进一步优选该芽孢杆菌属菌缺失或钝化了wprA或bpr。
作为使基因缺失或钝化的方法，可以列举：向该基因或其结构区导入突变等，例如该基因或其结构区的多核苷酸中的1个以上的核苷酸的置换或插入、或序列的部分或全部的敲除；通过其它序列向该基因的启动子区域的置换或插入而钝化该启动子；向该基因导入反义核酸等。作为用于该导入突变的具体方法，可以列举紫外线照射、定点突变导入、SOE-PCR法(重叠延伸剪接(splicing by overlap extension)PCR：Gene，1989，77：61-68)、同源重组法等。所缺失或钝化的基因在基因组DNA上的位置或核苷酸序列可以利用前述的BSORF DB进行确认。
在本发明中，使用按照前述的方法得到的导入了编码VHH的多核苷酸并且缺损细胞外蛋白酶的重组芽孢杆菌属菌。在该重组芽孢杆菌属菌的制备方法中，只要能够得到目标重组芽孢杆菌属菌，编码VHH的多核苷酸的导入和蛋白酶缺损处理的顺序没有特别限定。
在本发明中，对前述的导入了编码VHH的多核苷酸并且缺损细胞外蛋白酶的重组芽孢杆菌属菌进行培养，制造目标VHH。该重组芽孢杆菌属菌按照一般的芽孢杆菌属菌的培养方法进行培养即可。例如，用于培养芽孢杆菌属菌的培养基含有菌的生长所需的碳源和氮源。作为碳源，例如可以列举葡萄糖、葡聚糖、可溶性淀粉、蔗糖、甲醇等。作为氮源，例如可以列举铵盐类、硝酸盐类、氨基酸、玉米浆(corn steep liquor)、蛋白胨、酪蛋白、肉提取物、大豆粕、马铃薯提取液等。根据需要，该培养基也可以含有其它营养素，例如无机盐(例如氯化钠、氯化钙、磷酸二氢钠、氯化镁)、维生素类、抗生物质(例如四环素、新霉素、卡那霉素、大观霉素、红霉素等)等。培养条件、例如温度、通气搅拌条件、培养基的pH和培养时间等可以根据菌种、特质、培养规模等适当选择。
通过培养该重组芽孢杆菌属菌，VHH在细胞内表达。另外，在该VHH的基因与分泌信号连接的情况下，所表达的VHH分泌至细胞外。利用本发明的方法制造的VHH可以通过单独或适当组合使用用于蛋白质精制的一般方法、例如细胞的破碎、离心分离、硫酸铵沉淀、凝胶色谱、离子交换色谱、亲和色谱等而从培养液中回收。
作为例示的实施方式，本发明还包含以下的物质、制造方法、用途、方法等。但是，本发明并不限定于这些实施方式。
〔1〕一种重链抗体的重链可变区的制造方法，其中，所述方法包括对芽孢杆菌属菌进行培养的步骤，该芽孢杆菌属菌导入有编码重链抗体的重链可变区的基因，并且缺损细胞外蛋白酶。
〔2〕如〔1〕所述的方法，其中，上述细胞外蛋白酶优选为选自epr、wprA、bpr、vpr、aprE、aprX和与它们相当的基因所编码的细胞外蛋白酶中的至少1种，更优选为epr、wprA、bpr、vpr、aprE或aprX所编码的细胞外蛋白酶，进一步优选为wprA或bpr所编码的细胞外蛋白酶。
〔3〕如〔1〕或〔2〕所述的方法，其中，优选上述重链抗体的重链可变区由序列号2、4、6、8、10、12、14、16、18、20、22、24、26、28、30、32、34、36、38、64、66、68、70、72、74、76、78、80、82、84、86、88、90、92、94、96、98、100、102、104、106、108、110、112、114、116、118、120、122、124、126、128、130、132、134、136或138的氨基酸序列、或与该序列至少85％相同的氨基酸序列构成。
〔4〕如〔1〕～〔3〕中任一项所述的方法，其中，优选上述芽孢杆菌属菌为枯草杆菌或其突变株。
〔5〕如〔1〕～〔4〕中任一项所述的方法，其中，优选缺损上述细胞外蛋白酶的芽孢杆菌属菌为编码该细胞外蛋白酶的基因缺失或钝化的芽孢杆菌属菌。
〔6〕一种芽孢杆菌属菌，其中，所述芽孢杆菌属菌导入有编码重链抗体的重链可变区的基因，并且缺损细胞外蛋白酶。
〔7〕如〔6〕所述的芽孢杆菌属菌，其中，上述细胞外蛋白酶优选为选自epr、wprA、bpr、vpr、aprE、aprX和与它们相当的基因所编码的细胞外蛋白酶中的至少1种，更优选为epr、wprA、bpr、vpr、aprE或aprX所编码的细胞外蛋白酶，进一步优选为wprA或bpr所编码的细胞外蛋白酶。
〔8〕如〔6〕或〔7〕所述的芽孢杆菌属菌，其中，优选上述重链抗体的重链可变区由序列号2、4、6、8、10、12、14、16、18、20、22、24、26、28、30、32、34、36、38、64、66、68、70、72、74、76、78、80、82、84、86、88、90、92、94、96、98、100、102、104、106、108、110、112、114、116、118、120、122、124、126、128、130、132、134、136或138的氨基酸序列、或与该序列至少85％相同的氨基酸序列构成。
〔9〕如〔6〕～〔8〕中任一项所述的芽孢杆菌属菌，其中，所述芽孢杆菌属菌优选为枯草杆菌或其突变株。
〔10〕如〔6〕～〔9〕中任一项所述的芽孢杆菌属菌，其中，优选编码上述细胞外蛋白酶的基因缺失或钝化。
实施例
以下，利用实施例对本发明更具体地进行说明。但是，本发明的技术范围并不限定于这些实施例。
材料
(1)菌株
利用枯草杆菌(Bacillus subtilis)168株(以下称为168株)，按照专利文献2所记载的方法制作细胞外蛋白酶基因(epr、wprA、mpr、nprB、bpr、nprE、vpr、aprE和aprX)的缺损株。以下，将所得到的细胞外蛋白酶单一缺损株分别记载为Δepr、ΔwprA、Δmpr、ΔnprB、Δbpr、ΔnprE、Δvpr、ΔaprE和ΔaprX。另外，按照日本专利第4336082所记载的方法，使缺损全部上述9种细胞外蛋白酶基因的枯草杆菌株Dpr9缺损参与芽孢形成的sigF基因。将所得到的细胞外蛋白酶多重缺损株记载为Dpr9ΔsigF。
大肠杆菌使用Rosetta-gami 2(DE3)pLysS株(Novagen)。
(2)培养基
·LB培养基：1％BactoTM胰蛋白胨(Tryptone)、0.5％BactoTM酵母提取物(YeastExtract)、1％氯化钠。在平板培养基中添加1.5％的琼脂。根据需要添加四环素(50ppm)。
·DM3培养基：1％CMC(关东化学)、0.5％BactoTM酪蛋白氨基酸(Casamino Acids)、0.5％BactoTM酵母提取物(Yeast Extract)、8.1％琥珀酸二钠·6H2O、0.35％磷酸氢二钾、0.15％磷酸二氢钾、0.5％葡萄糖、20mM氯化镁、0.01％BSA、50ppm四环素。在平板培养基中添加1％的琼脂。
·2×L-mal培养基：2％BactoTM胰蛋白胨(Tryptone)、1％BactoTM酵母提取物(Yeast Extract)、1％氯化钠、7.5％麦芽糖一水合物、7.5ppm硫酸锰、15ppm四环素。
(3)试剂
没有特别记述时，试剂使用Wako公司制造的制品。
实施例1利用蛋白酶缺损重组枯草杆菌生产VHH
(1)基因的人工合成
VHH基因(序列号1、3、5、7、9、11、13、15、17、19、21、23、25、27、29、31、33、35和37的多核苷酸，编码各序列号2、4、6、8、10、12、14、16、18、20、22、24、26、28、30、32、34、36和38的VHH)由Thermo Fisher公司人工合成。在人工合成基因中，作为用于将该基因插入质粒载体的序列，GCAGCTCTTGCAGCA(序列号45)添加在各自的5’末端，TCTATTAAACTAGTT(序列号46)添加在各自的3’末端。
(2)VHH表达用质粒的构建
将重组质粒pHY-S237(日本特开2014-158430)作为模板，利用使用序列号47(5’-TGCTGCAAGAGCTGCCGGAAATAAA-3’)以及序列号48(5’-TCTATTAAACTAGTTATAGGG-3’)的引物组和PrimeSTAR Max DNA聚合酶(TaKaRa)的PCR，扩增质粒序列。使用In-Fusion HDCloning Kit(Takara)，将含有(1)的人工合成基因的DNA导入所得到的PCR片段，构建分别含有(1)所示的人工合成VHH基因的VHH表达用质粒。将所构建的质粒按照下述(3)所示的方法导入Dpr9ΔsigF中。
对于编码序列号26的VHH的基因，按照以下的方法将启动子区域变更为来自枯草杆菌spoVG基因的区域。将含有序列号25的基因的VHH表达用质粒作为模板，利用使用序列号49(5’-GATCCCCGGGAATTCCTGTTATAAAAAAAGG-3’)和序列号50(5’-ATGATGTTAAGAAAGAAAACAAAGCAG-3’)的引物组以及PrimeSTAR Max DNA聚合酶(TaKaRa)的PCR，扩增质粒序列。将168株的基因组作为模板，利用使用序列号51(5’-GAATTCCCGGGGATCTAAGAAAAGTGATTCTGGGAGAG-3’)和序列号52(5’-CTTTCTTAACATCATAGTAGTTCACCACCTTTTCCC-3’)的引物组的PCR，扩增来自spoVG基因的启动子DNA。使用In-Fusion HD Cloning Kit(Takara)，将所得到的启动子DNA导入质粒序列中，构建与spoVG启动子连接的含有序列号25的VHH基因的VHH表达用质粒。按照下述(3)所示的方法将所构建的质粒分别导入蛋白酶单一缺损株Δepr、ΔwprA、Δmpr、ΔnprB、Δbpr、ΔnprE、Δvpr、ΔaprE和ΔaprX中。
(3)重组枯草杆菌的制作
向枯草杆菌株的质粒导入利用以下所示的原生质体法进行。将甘油中保存的各种枯草杆菌植菌于1mL的LB液体培养基，以30℃、210rpm振荡培养一晩。第二日，将该培养液10μL植菌于新的1mL的LB液体培养基，以37℃、210rpm振荡培养约2小时。将该培养液回收至1.5mL管中，以1,2000rpm离心5分钟，将除去上清液的粒料悬浮在含有溶酶菌(Lysozyme)(SIGMA)4mg/mL的SMMP 500μL中，以37℃培养1小时。接着，以3,500rpm离心10分钟，将除去上清液的粒料悬浮在SMMP 400μL中。将该悬浮液33μL与各种质粒混合，再添加40％PEG 100μL并旋涡混合。向该液体中添加SMMP 350μL并倒转混合，以30℃、210rpm振荡1小时后，全部涂布在DM3琼脂培养基板上，以30℃进行2～3日培养。
(4)VHH生产
将(3)所制作的重组枯草杆菌植菌于1mL的含有50ppm四环素的LB培养基，以32℃往复振荡一晩，制成前培养液。对于蛋白酶单一缺损株，将前培养液在放入试验管中的5mL的2×L-mal培养基上进行1％接种，以30℃振荡培养72小时。对于Dpr9ΔsigF，将前培养液在放入具挡板三角烧瓶内的20mL的2×L-mal培养基上进行1％接种，以30℃振荡培养72小时。培养结束时，将1mL的培养液在微型管中以4℃、15,000rpm进行5分钟离心，回收上清液。
(5)SDS-PAGE
将(4)所得到的培养上清液与含有50mM DTT的Laemmli Sample Buffer(BIO-RAD)等量混合后，以99℃进行5分钟热处理，制备样品。凝胶使用Mini-PROTEIN TGX Stain-Free(BIO-RAD)。在各孔中涂布5μL的样品后，以210V进行25分钟电泳。分子量标记使用Precision Plus protein Unstained standard(BIO-RAD)。利用ChemiDoc MP ImagingSystem检测蛋白质的条带。利用以溶酶菌标品(Sigma-Aldrich)为基础制成的校正曲线，对检测到的蛋白质进行定量。蛋白质的定量使用BIO-RAD公司制造的软件Image Lab。
(6)蛋白质印迹法
利用SDS-PAGE凝胶，使用Trans-Blot Turbo Mini PVDF Transfer Packs(BIO-RAD)和Trans-Blot Turbo System(BIO-RAD)，将蛋白质转录于PVDF膜。抗体使用6×-His标签单抗(6×-His Tag Monoclonal Antibody)(3D5)、HRP(Invitorogen)，抗体反应使用iBind Western System(Invitrogen)。使用1-Step Ultra TMB-Blotting Solution(Thermo Scientific)检测目标蛋白质。使用以浓度已知的His-N15精制蛋白质(序列号26)为基础制成的校正曲线，对蛋白质进行定量。蛋白质的定量使用BIO-RAD公司制造的软件Image Lab。
(7)分子间相互作用测定
利用使用Biacore T200(GE Healthcare)的分子间相互作用测定，对利用Dpr9ΔsigF生产的1ZVH(序列号38)的抗原结合活性进行分析。传感器芯片使用Series S SensorChip CM5，电泳缓冲液(Running Buffer)使用HBS-EP+。利用25mM磷酸Na、pH6.5缓冲液将HEWL(溶酶菌，来自蛋清；Wako)调节至0.7g/L，制备抗原溶液。利用乙酸水溶液(pH4.0、4.5、5.0或5.5)对该抗原溶液进行100倍稀释，从而调节至7μg/mL，作为底物使用。将利用电泳缓冲液稀释至8.6nM、26nM、78nM、230nM或700nM的1ZVH作为样品。使用该底物和样品，在Biacore T200附带的控制软件上设定或施行以下的项目，监测HEWL与1ZVH间的结合。施行Immobilisation pH Scouting，对最适于向传感器芯片的固定化的pH进行研究。判断最适pH条件为pH5.5。利用Aim for immobilized level，将目标设为150RU，利用胺偶联法，将溶酶菌固定于流路(Flow path)2。另外，流路(Flow path)1实施Blank Immobilization。在流路(Flow path)2-1监测底物与样品的结合。再生液(Regeneration Solution)使用10mM甘氨酸-盐酸(Glycine-HCl)(pH1.5)。结合监测时的接触时间(Contact time)为120sec，流速(Flow rate)为30μL/min，解离时间(Dissociation time)为120sec，再生(Regeneration)的接触时间(Contact time)为30sec，稳定期(Stabilization period)为120sec。使用所得到的传感图，进行动力学(Kinetics)分析。反应模式选择1：1Binding。
(8)结果
将关于来自细胞外蛋白酶单一缺损重组枯草杆菌株Δepr、ΔwprA、Δmpr、ΔnprB、Δbpr、ΔnprE、Δvpr、ΔaprE和ΔaprX的培养上清液的SDS-PAGE图像示于图1。图中的“+”表示抗体的条带(图中用箭头表示)的强度。在Δepr、ΔwprA、Δbpr、Δvpr、ΔaprE和ΔaprX中VHH高表达。其中，ΔwprA和Δbpr中的VHH表达显著高。根据这些结果，显示epr、wprA、bpr、vpr、aprE和aprX的缺损影响了枯草杆菌中的VHH表达。
表3显示细胞外蛋白酶多重缺损重组枯草杆菌株Dpr9ΔsigF中的序列号2、4、6、8、10、12、14、16、18、20、22、24、26和28的VHH的表达量。利用蛋白质印迹法对序列号4和序列号28的表达进行定量，利用SDS-PAGE对剩余的表达进行定量。利用Dpr9ΔsigF能够生产各种VHH。另外，图2显示Dpr9ΔsigF中的序列号30、32的医药VHH分子(奥利组单抗和卡普赛珠单抗)、以及序列号34、36的医药用人源化VHH分子(NbHul6_VGLW和h-NbBcII10_FGLA)的表达量。图3显示Dpr9ΔsigF所表达的奥利组单抗和卡普赛珠单抗的蛋白质印迹图像。Dpr9ΔsigF能够以全长表达全部4种医药VHH分子(参照图2和图3)。根据这些结果，显示蛋白酶缺损枯草杆菌株能够表达连接有多个VHH、人源化VHH和VHH的分子等。
[表3]
VHH 序列号 (g/L)
3G9A 2 0.31
4C57 4 0.3
5IMM 6 0.88
5JQH 8 0.41
5NBD 10 0.39
3k1k 12 0.51
4FHB 14 1.76
4KSD 16 0.11
3K81 18 0.86
5FV2 20 0.19
4W6W 22 0.16
5J57 24 0.12
N15 26 0.49
4GFT 28 0.02
图4显示Dpr9ΔsigF的培养上清液和精制中得到的组分的电泳图像(左)以及(7)中测得的精制后的VHH(1ZVH、序列号38)的抗原(HEWL)结合活性(右)。在电泳中，将Dpr9ΔsigF培养上清液(S)供于填充有Ni-NTA琼脂糖(Agarose)(Wako)的柱，得到通过柱的组分FT。接着，将清洗缓冲液(30mM咪唑、0.25M NaCl、10mM Tris pH8.0)供于柱，得到通过柱的组分W。之后，将洗脱缓冲液(500mM咪唑、0.5M NaCl、20mM Tris pH7.5)供于柱，得到通过柱的组分E。利用不含DTT的Laemmli Sample Buffer(BIO-RAD)对所得到的S和组分FT、W、E进行处理。另外，利用含有50mM DTT的Laemmli Sample Buffer(BIO-RAD)对组分E进行处理。将处理过的样品供于SDS-PAGE。与利用不含DTT的缓冲液进行了处理的组分E(非还原E)相比，在利用含有DTT的缓冲液进行了处理的组分E(还原E)中，条带向上方移动。根据该结果，显示在枯草杆菌所表达的VHH内形成了二硫键。另外，如图4右所示，通过分子间相互作用测定，观察到所表达的VHH对固定在浓度依赖性传感器上的抗原的响应(response)值上升，因此保持了抗原结合活性。根据该结果，显示使用枯草杆菌能够生产活性型VHH。
比较例1利用重组大肠杆菌的VHH生产
(1)重组大肠杆菌的制作
利用使用表4所示的引物和PrimeSTAR Max DNA聚合酶(TaKaRa)的PCR，扩增含有实施例1(1)所示的人工合成基因(序列号1、3、5、7和9的多核苷酸，编码各序列号2、4、6、8和10的VHH)的DNA。利用Nco I和Hind III消化pET22b，使用In-Fusion HD Cloning Kit导入所得到的PCR片段，构建质粒。将所构建的质粒与Rosetta-gami 2(DE3)pLysS株的感受态细胞混合，在冰上静置10分钟后，以42℃进行1分钟处理，从而导入大肠杆菌株中。
[表4]
Figure BDA0003766872860000181
(2)VHH生产
将(1)所制作的重组大肠杆菌植菌于含有2mL的50ppm氨苄西林钠的LB培养基上，以30℃往复振荡一晩，制成前培养液。将20μL的前培养液植菌于2mL的Overnight ExpressTMInstant TB Medium上，以30℃进行24小时培养。
(3)SDS-PAGE和蛋白质印迹法
利用(2)所得到的培养物，通过离心进行集菌。将所得到的菌体再悬浮在与供于离心的培养液等量的PBS缓冲液中，向其中添加含有50mM DTT的Laemmli Sample Buffer(BIO-RAD)，制备样品。按照与实施例1(5)相同的方法将所得到的样品供于SDS-PAGE。蛋白质印迹法按照与实施例1(6)相同的方法进行。
(4)结果
将关于大肠杆菌株培养物的SDS-PAGE图像示于图5。作为蛋白质定量的结果，任意株均为15mg/L以下的生产率。与Dpr9ΔsigF株中的VHH表达量(表3)进行比较时，显示枯草杆菌的VHH生产率为大肠杆菌的20倍以上。
实施例2利用蛋白酶缺损重组枯草杆菌的VHH生产
作为VHH基因，使用序列号63、65、67、69、71、73、75、77、79、81、83、85、87、89、91、93、95、97、99、101、103、105、107、109、111、113、115、117、119、121、123、125、127、129、131、133、135和137的多核苷酸(编码各序列号64、66、68、70、72、74、76、78、80、82、84、86、88、90、92、94、96、98、100、102、104、106、108、110、112、114、116、118、120、122、124、126、128、130、132、134、136和138的VHH：分别称为VHH1～38)，除此以外，按照与实施例1(1)和(2)相同的方法构建VHH表达用质粒。将所构建的质粒按照实施例1(3)所示的方法导入Dpr9ΔsigF中。按照与实施例1(4)相同的方法培养所制作的重组枯草杆菌，使VHH表达。
利用ELISA对由重组枯草杆菌表达的VHH进行定量。ELISA按照His标签抗体板(HisTag Antibody Plate)(GenScript公司)的手册进行。将上述所得到的重组枯草杆菌的培养物的上清液用PBS稀释至2万倍，将100μL稀释液加入His标签抗体板(His Tag AntibodyPlate)的各孔中，以4℃静置一晩。利用添加有Tween(注册商标)20 0.05％的PBS(PBST)将孔清洗3次，接着，添加用PBST稀释5000倍的Peroxidase-conjugated AffiniPure GoatAnti-Alpaca IgG、VHH区(Jackson ImmunoResearch 128-035-232)100μL，室温下静置30分钟。之后，利用PBST将孔清洗3次，添加3,3',5,5'-四甲基联苯胺(TMB[ELISA用]；东京化成工业)100μL，室温下静置20分钟。添加TMB停止液(stop solution)(SeraCare LifeSciences)50μL使反应停止后，测定各孔的450nm的吸光度。按照Ni-NTA琼脂糖(富士胶片和光纯药)的手册，将浓度已知的VHH1的ELISA的吸光度作为指标，算出蛋白质浓度。
表5显示重组枯草杆菌株Dpr9ΔsigF中的VHH1～38的表达量。VHH1～38均由重组枯草杆菌株生产。
[表5]
VHH 序列号 表达量(g/L) VHH 序列号 表达量(g/L)
VHH1 64 >2 VHH20 102 1.2
VHH2 66 1.7 VHH21 104 >2
VHH3 68 >2 VHH22 106 >2
VHH4 70 2.0 VHH23 108 >2
VHH5 72 >2 VHH24 110 >2
VHH6 74 >2 VHH25 112 >2
VHH7 76 1.1 VHH26 114 >2
VHH8 78 1.0 VHH27 116 >2
VHH9 80 1.2 VHH28 118 1.65
VHH10 82 >2 VHH29 120 1.15
VHH11 84 >2 VHH30 122 0.16
VHH12 86 1.94 VHH31 124 1.74
VHH13 88 0.45 VHH32 126 0.39
VHH14 90 1.67 VHH33 128 0.1
VHH15 92 >2 VHH34 130 0.11
VHH16 94 >2 VHH35 132 0.08
VHH17 96 >2 VHH36 134 >2
VHH18 98 1.04 VHH37 136 0.37
VHH19 100 1.9 VHH38 138 1.13
※>2表示2g/L以上。校正曲线上限以外。
以上，对本发明的实施方式进行了说明，但能够理解的是这些并没有将本发明限定于所说明的特定的实施方式的意图。在本发明的范围内进行的各种其它的变更和修正对于本领域技术人员是清楚的。本说明书所引用的文献和专利申请正如其在本说明书中完全记载的那样，作为参考而被引用。
序列表
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<120> 重链抗体的重链可变区的制造方法
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1 5 10 15
tca ctg aga ctg tca tgc gca gca tca ggc aga aca ttt tca tca tat 96
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Thr Phe Ser Ser Tyr
20 25 30
ggc atg ggc tgg ttt aga caa gca ccg gga aaa gaa aga gaa ttt gtt 144
Gly Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Phe Val
35 40 45
gca gca att aga tgg aat ggc gga agc aca tat tat gca gat tca gtc 192
Ala Ala Ile Arg Trp Asn Gly Gly Ser Thr Tyr Tyr Ala Asp Ser Val
50 55 60
aaa gga cgc ttt aca att agc aga gat aac gcg aaa aac acg gtc tat 240
Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Val Tyr
65 70 75 80
ctg caa atg aat tca ctg aaa ccg gaa gat aca gcg gtc tat tat tgc 288
Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys
85 90 95
gca gca ggc aga tgg gat aaa tat ggc tca tca ttt cag gac gaa tat 336
Ala Ala Gly Arg Trp Asp Lys Tyr Gly Ser Ser Phe Gln Asp Glu Tyr
100 105 110
gac tat tgg gga caa ggc aca caa gtt aca gtt tca tca cat cat cac 384
Asp Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser His His His
115 120 125
cat cac cac taa a 397
His His His
130
<210> 6
<211> 131
<212> PRT
<213> 未知(Unknown)
<220>
<223> 骆驼科(Camelidae)
<400> 6
Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Gly
1 5 10 15
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Thr Phe Ser Ser Tyr
20 25 30
Gly Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Phe Val
35 40 45
Ala Ala Ile Arg Trp Asn Gly Gly Ser Thr Tyr Tyr Ala Asp Ser Val
50 55 60
Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Val Tyr
65 70 75 80
Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys
85 90 95
Ala Ala Gly Arg Trp Asp Lys Tyr Gly Ser Ser Phe Gln Asp Glu Tyr
100 105 110
Asp Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser His His His
115 120 125
His His His
130
<210> 7
<211> 379
<212> DNA
<213> 大羊驼(Lama glama)
<220>
<221> CDS
<222> (1)..(378)
<400> 7
caa gtc caa ctg caa gaa tca ggc gga ggc ctg gtt caa gca ggc gga 48
Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Gly
1 5 10 15
tca ctg aga ctg tca tgc gca gca tca ggc tca att ttt tca ctg aat 96
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Ser Ile Phe Ser Leu Asn
20 25 30
gat atg ggc tgg tat aga caa gca ccg gga aaa ctg aga gaa ctg gtt 144
Asp Met Gly Trp Tyr Arg Gln Ala Pro Gly Lys Leu Arg Glu Leu Val
35 40 45
gca gca att aca agc gga ggc tca aca aaa tat gca gat tca gtt aaa 192
Ala Ala Ile Thr Ser Gly Gly Ser Thr Lys Tyr Ala Asp Ser Val Lys
50 55 60
gga cgc ttt acg att agc aga gat aac gcg aaa aat acg gtc tac ctg 240
Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Val Tyr Leu
65 70 75 80
caa atg aat tca ctg aaa gca gaa gat aca gcg gtc tat tat tgc aat 288
Gln Met Asn Ser Leu Lys Ala Glu Asp Thr Ala Val Tyr Tyr Cys Asn
85 90 95
gca aaa gtt gca ggc acg ttt agc atc tat gat tat tgg gga caa ggc 336
Ala Lys Val Ala Gly Thr Phe Ser Ile Tyr Asp Tyr Trp Gly Gln Gly
100 105 110
aca caa gtt aca gtt tca tca cat cat cac cat cac cac taa a 379
Thr Gln Val Thr Val Ser Ser His His His His His His
115 120 125
<210> 8
<211> 125
<212> PRT
<213> 大羊驼(Lama glama)
<400> 8
Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Gly
1 5 10 15
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Ser Ile Phe Ser Leu Asn
20 25 30
Asp Met Gly Trp Tyr Arg Gln Ala Pro Gly Lys Leu Arg Glu Leu Val
35 40 45
Ala Ala Ile Thr Ser Gly Gly Ser Thr Lys Tyr Ala Asp Ser Val Lys
50 55 60
Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Val Tyr Leu
65 70 75 80
Gln Met Asn Ser Leu Lys Ala Glu Asp Thr Ala Val Tyr Tyr Cys Asn
85 90 95
Ala Lys Val Ala Gly Thr Phe Ser Ile Tyr Asp Tyr Trp Gly Gln Gly
100 105 110
Thr Gln Val Thr Val Ser Ser His His His His His His
115 120 125
<210> 9
<211> 391
<212> DNA
<213> 大羊驼(Lama glama)
<220>
<221> CDS
<222> (1)..(390)
<400> 9
caa gtc caa ctg caa gaa tca ggc gga ggc ctg gtt caa cct ggc gga 48
Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
1 5 10 15
tca ctg aga ctg tca tgc gca gca tca ggc ttt aca ttt tca tca tat 96
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
20 25 30
gca atg agc tgg gtt aga caa gca cct ggc aaa ggc ctg gaa tgg gtt 144
Ala Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
35 40 45
tca gca att aca tct ggc gga ggc tca aca tca tat tca gat gca gtt 192
Ser Ala Ile Thr Ser Gly Gly Gly Ser Thr Ser Tyr Ser Asp Ala Val
50 55 60
aaa gga cgc ttt aca atc agc aga gat aac gcg aaa aat acg ctg tat 240
Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Leu Tyr
65 70 75 80
ctg caa atg aat tca ctg aaa ccg gaa gat acg gca gtc tat tat tgc 288
Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys
85 90 95
gca aaa ggc tat gtt gtc gat ttt ctg gat ctg gca gaa tat gat tca 336
Ala Lys Gly Tyr Val Val Asp Phe Leu Asp Leu Ala Glu Tyr Asp Ser
100 105 110
tgg gga caa ggc aca caa gtt aca gtt tca agc cat cac cat cat cat 384
Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser His His His His His
115 120 125
cat taa a 391
His
<210> 10
<211> 129
<212> PRT
<213> 大羊驼(Lama glama)
<400> 10
Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
1 5 10 15
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
20 25 30
Ala Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
35 40 45
Ser Ala Ile Thr Ser Gly Gly Gly Ser Thr Ser Tyr Ser Asp Ala Val
50 55 60
Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Leu Tyr
65 70 75 80
Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys
85 90 95
Ala Lys Gly Tyr Val Val Asp Phe Leu Asp Leu Ala Glu Tyr Asp Ser
100 105 110
Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser His His His His His
115 120 125
His
<210> 11
<211> 370
<212> DNA
<213> 单峰驼(Camelus dromedarius)
<220>
<221> CDS
<222> (1)..(369)
<400> 11
gca caa gtt caa ctg gtt gaa tca ggc gga gca ctg gtt caa cct ggc 48
Ala Gln Val Gln Leu Val Glu Ser Gly Gly Ala Leu Val Gln Pro Gly
1 5 10 15
gga tca ctg aga ctg tca tgc gca gca tca ggc ttt ccg gtt aat aga 96
Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Pro Val Asn Arg
20 25 30
tat tca atg aga tgg tat aga cag gca ccg gga aaa gaa aga gaa tgg 144
Tyr Ser Met Arg Trp Tyr Arg Gln Ala Pro Gly Lys Glu Arg Glu Trp
35 40 45
gtt gca ggc atg tca tca gca ggc gat aga tca tca tat gaa gat tca 192
Val Ala Gly Met Ser Ser Ala Gly Asp Arg Ser Ser Tyr Glu Asp Ser
50 55 60
gtc aaa gga cgc ttt aca atc tca aga gat gat gcg aga aat acg gtc 240
Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asp Ala Arg Asn Thr Val
65 70 75 80
tat ctg caa atg aat tca ctg aaa ccg gaa gat aca gcg gtc tat tat 288
Tyr Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr
85 90 95
tgc aat gtt aac gtc ggc ttt gaa tat tgg gga caa ggc aca caa gtt 336
Cys Asn Val Asn Val Gly Phe Glu Tyr Trp Gly Gln Gly Thr Gln Val
100 105 110
aca gtt tca tca cat cat cac cat cac cac taa a 370
Thr Val Ser Ser His His His His His His
115 120
<210> 12
<211> 122
<212> PRT
<213> 单峰驼(Camelus dromedarius)
<400> 12
Ala Gln Val Gln Leu Val Glu Ser Gly Gly Ala Leu Val Gln Pro Gly
1 5 10 15
Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Pro Val Asn Arg
20 25 30
Tyr Ser Met Arg Trp Tyr Arg Gln Ala Pro Gly Lys Glu Arg Glu Trp
35 40 45
Val Ala Gly Met Ser Ser Ala Gly Asp Arg Ser Ser Tyr Glu Asp Ser
50 55 60
Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asp Ala Arg Asn Thr Val
65 70 75 80
Tyr Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr
85 90 95
Cys Asn Val Asn Val Gly Phe Glu Tyr Trp Gly Gln Gly Thr Gln Val
100 105 110
Thr Val Ser Ser His His His His His His
115 120
<210> 13
<211> 409
<212> DNA
<213> 大羊驼(Lama glama)
<220>
<221> CDS
<222> (1)..(408)
<400> 13
caa gtc caa ctg caa gaa tca ggc gga ggc ctg gtt caa gca ggc gga 48
Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Gly
1 5 10 15
tca ctg aga ctg tca tgc gaa gca tca ggc aga aca ttt tca tca tat 96
Ser Leu Arg Leu Ser Cys Glu Ala Ser Gly Arg Thr Phe Ser Ser Tyr
20 25 30
gca atg ggc tgg ttt aga caa gca ccg gga aaa gaa aga gat ttt gtt 144
Ala Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Asp Phe Val
35 40 45
gca gtt att tca tgg tca ggc agc aac aca tat tat gca gat tca gca 192
Ala Val Ile Ser Trp Ser Gly Ser Asn Thr Tyr Tyr Ala Asp Ser Ala
50 55 60
aaa gga cgc ttt aca att agc aga gat aac gcg aaa aac acg gtc tat 240
Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Val Tyr
65 70 75 80
ctg caa atg aat tca ctg aaa ccg gaa gat acg gcg atc tat tat tgc 288
Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Ile Tyr Tyr Cys
85 90 95
gca gca cct ggc aga ccg cat ggc tca tca tgg tca tta aac aaa aaa 336
Ala Ala Pro Gly Arg Pro His Gly Ser Ser Trp Ser Leu Asn Lys Lys
100 105 110
ggc cag ggc tat gat tat tgg gga caa ggc aca caa gtt aca gtt tca 384
Gly Gln Gly Tyr Asp Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser
115 120 125
tca cat cat cac cat cac cac taa a 409
Ser His His His His His His
130 135
<210> 14
<211> 135
<212> PRT
<213> 大羊驼(Lama glama)
<400> 14
Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Gly
1 5 10 15
Ser Leu Arg Leu Ser Cys Glu Ala Ser Gly Arg Thr Phe Ser Ser Tyr
20 25 30
Ala Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Asp Phe Val
35 40 45
Ala Val Ile Ser Trp Ser Gly Ser Asn Thr Tyr Tyr Ala Asp Ser Ala
50 55 60
Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Val Tyr
65 70 75 80
Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Ile Tyr Tyr Cys
85 90 95
Ala Ala Pro Gly Arg Pro His Gly Ser Ser Trp Ser Leu Asn Lys Lys
100 105 110
Gly Gln Gly Tyr Asp Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser
115 120 125
Ser His His His His His His
130 135
<210> 15
<211> 376
<212> DNA
<213> 大羊驼(Lama glama)
<220>
<221> CDS
<222> (1)..(375)
<400> 15
caa gtc caa ctg caa gaa tca ggc gga ggc ctg gtt caa gca ggc gga 48
Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Gly
1 5 10 15
tca ctg aga ctg tca tgc gca gca tca ggc aga aca ttt aat tca gca 96
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Thr Phe Asn Ser Ala
20 25 30
gtt atg ggc tgg ttt aga caa gca ccg gga aaa gaa aga caa ttt gtt 144
Val Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Gln Phe Val
35 40 45
gcg aca att gat tgg tca ggc gaa tac aca tat tat gcg gat tca gtc 192
Ala Thr Ile Asp Trp Ser Gly Glu Tyr Thr Tyr Tyr Ala Asp Ser Val
50 55 60
aaa gga cgc ttt aca att tca cgc gat aat gcg aaa aac acg gtc tat 240
Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Val Tyr
65 70 75 80
ctg caa atg aca tca ctg aaa ccg gaa gat aca gca ctg tat tat tgc 288
Leu Gln Met Thr Ser Leu Lys Pro Glu Asp Thr Ala Leu Tyr Tyr Cys
85 90 95
gca gca aga ctg aca ctg ggc caa ttt gat tat tgg gga caa ggc aca 336
Ala Ala Arg Leu Thr Leu Gly Gln Phe Asp Tyr Trp Gly Gln Gly Thr
100 105 110
caa gtt aca gtt tca tca cat cat cac cat cac cac taa a 376
Gln Val Thr Val Ser Ser His His His His His His
115 120
<210> 16
<211> 124
<212> PRT
<213> 大羊驼(Lama glama)
<400> 16
Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Gly
1 5 10 15
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Thr Phe Asn Ser Ala
20 25 30
Val Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Gln Phe Val
35 40 45
Ala Thr Ile Asp Trp Ser Gly Glu Tyr Thr Tyr Tyr Ala Asp Ser Val
50 55 60
Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Val Tyr
65 70 75 80
Leu Gln Met Thr Ser Leu Lys Pro Glu Asp Thr Ala Leu Tyr Tyr Cys
85 90 95
Ala Ala Arg Leu Thr Leu Gly Gln Phe Asp Tyr Trp Gly Gln Gly Thr
100 105 110
Gln Val Thr Val Ser Ser His His His His His His
115 120
<210> 17
<211> 385
<212> DNA
<213> 大羊驼(Lama glama)
<220>
<221> CDS
<222> (1)..(384)
<400> 17
gaa gtt caa ccg caa gaa tca ggc gga ggc ctg gca caa gca ggc gga 48
Glu Val Gln Pro Gln Glu Ser Gly Gly Gly Leu Ala Gln Ala Gly Gly
1 5 10 15
tca ctg aga ctg tca tgc gtt gtt tca ggc att aca ttt gca tca gaa 96
Ser Leu Arg Leu Ser Cys Val Val Ser Gly Ile Thr Phe Ala Ser Glu
20 25 30
gca tgg ggc tgg tat aga cgc gca ccg gga aaa caa aga gaa ctg att 144
Ala Trp Gly Trp Tyr Arg Arg Ala Pro Gly Lys Gln Arg Glu Leu Ile
35 40 45
gca gca att aat aat gaa gga cgc acg aac tat gtc gat tca gtt aaa 192
Ala Ala Ile Asn Asn Glu Gly Arg Thr Asn Tyr Val Asp Ser Val Lys
50 55 60
ggc aga ttt aca gtc agc aga gat aat gcg aaa aac gtc atg tat ctg 240
Gly Arg Phe Thr Val Ser Arg Asp Asn Ala Lys Asn Val Met Tyr Leu
65 70 75 80
caa atg aac tca ctg aaa ccg gaa gat aca gca gtc tat tat tgc aat 288
Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys Asn
85 90 95
gca aat ctg caa aca ggc aca ctg tca ggc gca aga ctg tat tgg gga 336
Ala Asn Leu Gln Thr Gly Thr Leu Ser Gly Ala Arg Leu Tyr Trp Gly
100 105 110
caa ggc aca caa gtt aca gtt tca tca cat cat cac cat cac cac taa a 385
Gln Gly Thr Gln Val Thr Val Ser Ser His His His His His His
115 120 125
<210> 18
<211> 127
<212> PRT
<213> 大羊驼(Lama glama)
<400> 18
Glu Val Gln Pro Gln Glu Ser Gly Gly Gly Leu Ala Gln Ala Gly Gly
1 5 10 15
Ser Leu Arg Leu Ser Cys Val Val Ser Gly Ile Thr Phe Ala Ser Glu
20 25 30
Ala Trp Gly Trp Tyr Arg Arg Ala Pro Gly Lys Gln Arg Glu Leu Ile
35 40 45
Ala Ala Ile Asn Asn Glu Gly Arg Thr Asn Tyr Val Asp Ser Val Lys
50 55 60
Gly Arg Phe Thr Val Ser Arg Asp Asn Ala Lys Asn Val Met Tyr Leu
65 70 75 80
Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys Asn
85 90 95
Ala Asn Leu Gln Thr Gly Thr Leu Ser Gly Ala Arg Leu Tyr Trp Gly
100 105 110
Gln Gly Thr Gln Val Thr Val Ser Ser His His His His His His
115 120 125
<210> 19
<211> 370
<212> DNA
<213> 未知(Unknown)
<220>
<223> 未知物种(Unknown species)
<220>
<221> CDS
<222> (1)..(369)
<400> 19
gaa gtt caa ctg ctg gtt tca ggc gga ggc ctg gtt caa cct ggc gga 48
Glu Val Gln Leu Leu Val Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
1 5 10 15
tca ctg aga ctg tca tgc gca gca tca ggc ttt aca ttt aaa gca tat 96
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Lys Ala Tyr
20 25 30
ccg atg atg tgg gtt aga caa gca cct ggc aaa ggc ctg gaa tgg gtt 144
Pro Met Met Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
35 40 45
tca gaa att agc ccg tca ggc agc tat aca tat tat gca gat tca gtc 192
Ser Glu Ile Ser Pro Ser Gly Ser Tyr Thr Tyr Tyr Ala Asp Ser Val
50 55 60
aaa gga cgc ttt aca att agc cgt gat aac agc aaa aac acg ctg tat 240
Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
65 70 75 80
ctg caa atg aat tca ctg aga gca gaa gat acg gca gtc tat tat tgc 288
Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys
85 90 95
gca aaa gat ccg aga aaa ctg gat tat tgg gga caa ggc aca ctg gtt 336
Ala Lys Asp Pro Arg Lys Leu Asp Tyr Trp Gly Gln Gly Thr Leu Val
100 105 110
aca gtt tca tca cat cat cac cac cat cat taa a 370
Thr Val Ser Ser His His His His His His
115 120
<210> 20
<211> 122
<212> PRT
<213> 未知(Unknown)
<220>
<223> 未知物种(Unknown species)
<400> 20
Glu Val Gln Leu Leu Val Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
1 5 10 15
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Lys Ala Tyr
20 25 30
Pro Met Met Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
35 40 45
Ser Glu Ile Ser Pro Ser Gly Ser Tyr Thr Tyr Tyr Ala Asp Ser Val
50 55 60
Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
65 70 75 80
Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys
85 90 95
Ala Lys Asp Pro Arg Lys Leu Asp Tyr Trp Gly Gln Gly Thr Leu Val
100 105 110
Thr Val Ser Ser His His His His His His
115 120
<210> 21
<211> 397
<212> DNA
<213> 大羊驼(Lama glama)
<220>
<221> CDS
<222> (1)..(396)
<400> 21
caa gtc caa ctg caa gaa tca ggc gga ggc tca gtt caa gca ggc gga 48
Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Ser Val Gln Ala Gly Gly
1 5 10 15
tca ctg aga ctg tca tgc gca gca tca ggc tat aca tca ggc aga gat 96
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Tyr Thr Ser Gly Arg Asp
20 25 30
tca atg ggc tgg ttt aga caa gca ccg gga aaa gaa aga gaa ggc gtt 144
Ser Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Gly Val
35 40 45
gct tgc att gat aca agc ggc att gtt aat tat gcg gat tca gtc aaa 192
Ala Cys Ile Asp Thr Ser Gly Ile Val Asn Tyr Ala Asp Ser Val Lys
50 55 60
gga cgc ttt aca att tca caa gac agc gcg aaa aaa acg ctg tat ctg 240
Gly Arg Phe Thr Ile Ser Gln Asp Ser Ala Lys Lys Thr Leu Tyr Leu
65 70 75 80
gaa atg aat tca ctg aaa ccg gaa gat aca gca ctg tat tca tgc gca 288
Glu Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Leu Tyr Ser Cys Ala
85 90 95
aca gga ccg ttt gtt tat ggc aga ggc tgc ctg ggc caa gca ttt tat 336
Thr Gly Pro Phe Val Tyr Gly Arg Gly Cys Leu Gly Gln Ala Phe Tyr
100 105 110
tca tat tgg gga caa ggc aca caa gtt aca gtt tca tca cat cat cac 384
Ser Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser His His His
115 120 125
cat cac cac taa a 397
His His His
130
<210> 22
<211> 131
<212> PRT
<213> 大羊驼(Lama glama)
<400> 22
Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Ser Val Gln Ala Gly Gly
1 5 10 15
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Tyr Thr Ser Gly Arg Asp
20 25 30
Ser Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Gly Val
35 40 45
Ala Cys Ile Asp Thr Ser Gly Ile Val Asn Tyr Ala Asp Ser Val Lys
50 55 60
Gly Arg Phe Thr Ile Ser Gln Asp Ser Ala Lys Lys Thr Leu Tyr Leu
65 70 75 80
Glu Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Leu Tyr Ser Cys Ala
85 90 95
Thr Gly Pro Phe Val Tyr Gly Arg Gly Cys Leu Gly Gln Ala Phe Tyr
100 105 110
Ser Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser His His His
115 120 125
His His His
130
<210> 23
<211> 403
<212> DNA
<213> 羊驼(Vicugna pacos)
<220>
<221> CDS
<222> (1)..(402)
<400> 23
gtt caa ctg gca gaa aca ggc gga ggc ctg gtt gaa cct ggc gga tca 48
Val Gln Leu Ala Glu Thr Gly Gly Gly Leu Val Glu Pro Gly Gly Ser
1 5 10 15
ctg aga ctg tca tgc gca gca ccg gaa ttt aga ctg cag tat tat aca 96
Leu Arg Leu Ser Cys Ala Ala Pro Glu Phe Arg Leu Gln Tyr Tyr Thr
20 25 30
gca ggc tgg ttt aga caa gca ccg gga aaa gaa aga gaa tgg gtt gct 144
Ala Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Trp Val Ala
35 40 45
tgc att tca gct ggc gga ggc gtt aca tat tat acg ggc tca gtt caa 192
Cys Ile Ser Ala Gly Gly Gly Val Thr Tyr Tyr Thr Gly Ser Val Gln
50 55 60
ggc cgt ttt aca att tca aga gat aat gca aaa cgc acg gtc tat ctg 240
Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Arg Thr Val Tyr Leu
65 70 75 80
caa atg gat tca ctg aaa ccg gaa gat aca gca gtt tat tct tgc gca 288
Gln Met Asp Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Ser Cys Ala
85 90 95
gca gat ctg gaa tat tca caa att atg ccg tca tgc aga ggc tca tat 336
Ala Asp Leu Glu Tyr Ser Gln Ile Met Pro Ser Cys Arg Gly Ser Tyr
100 105 110
ggc gtt aga ggc caa ggc aca caa gtt aca gtt tca tca gca cat cat 384
Gly Val Arg Gly Gln Gly Thr Gln Val Thr Val Ser Ser Ala His His
115 120 125
cac cat cac cac cat taa a 403
His His His His His
130
<210> 24
<211> 133
<212> PRT
<213> 羊驼(Vicugna pacos)
<400> 24
Val Gln Leu Ala Glu Thr Gly Gly Gly Leu Val Glu Pro Gly Gly Ser
1 5 10 15
Leu Arg Leu Ser Cys Ala Ala Pro Glu Phe Arg Leu Gln Tyr Tyr Thr
20 25 30
Ala Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Trp Val Ala
35 40 45
Cys Ile Ser Ala Gly Gly Gly Val Thr Tyr Tyr Thr Gly Ser Val Gln
50 55 60
Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Arg Thr Val Tyr Leu
65 70 75 80
Gln Met Asp Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Ser Cys Ala
85 90 95
Ala Asp Leu Glu Tyr Ser Gln Ile Met Pro Ser Cys Arg Gly Ser Tyr
100 105 110
Gly Val Arg Gly Gln Gly Thr Gln Val Thr Val Ser Ser Ala His His
115 120 125
His His His His His
130
<210> 25
<211> 381
<212> DNA
<213> 小羊驼(Lama pacos)
<220>
<221> CDS
<222> (1)..(381)
<400> 25
cag gtt cag ctt gtt gag tct gga ggt ggg ctt gta caa cca ggt ggc 48
Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
1 5 10 15
tct ttg cgc tta agc tgt gct gca tct ggc ttt acg ttc tcg aat gat 96
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Asn Asp
20 25 30
gcc atg aca tgg gta cgt caa gca cct gga aaa gct ctg gaa tgg gtg 144
Ala Met Thr Trp Val Arg Gln Ala Pro Gly Lys Ala Leu Glu Trp Val
35 40 45
tca tcc att acc ctc tta gga gga gga att ccg tat tac agc gac aca 192
Ser Ser Ile Thr Leu Leu Gly Gly Gly Ile Pro Tyr Tyr Ser Asp Thr
50 55 60
gtt aaa ggc cgg ttt acc atc tca agg gat aac acg aag aac atg ctg 240
Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Thr Lys Asn Met Leu
65 70 75 80
tac ttg cag atg aat agc ctg aaa ccg gaa gat aca gcg gtg tat tat 288
Tyr Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr
85 90 95
tgc gcg aaa ggc ttt aag tcc gac tat ccc aga ggt caa ggg aca caa 336
Cys Ala Lys Gly Phe Lys Ser Asp Tyr Pro Arg Gly Gln Gly Thr Gln
100 105 110
gtc act gtc agt tca cga ggc aga cat cac cac cat cat cat taa 381
Val Thr Val Ser Ser Arg Gly Arg His His His His His His
115 120 125
<210> 26
<211> 126
<212> PRT
<213> 小羊驼(Lama pacos)
<400> 26
Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
1 5 10 15
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Asn Asp
20 25 30
Ala Met Thr Trp Val Arg Gln Ala Pro Gly Lys Ala Leu Glu Trp Val
35 40 45
Ser Ser Ile Thr Leu Leu Gly Gly Gly Ile Pro Tyr Tyr Ser Asp Thr
50 55 60
Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Thr Lys Asn Met Leu
65 70 75 80
Tyr Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr
85 90 95
Cys Ala Lys Gly Phe Lys Ser Asp Tyr Pro Arg Gly Gln Gly Thr Gln
100 105 110
Val Thr Val Ser Ser Arg Gly Arg His His His His His His
115 120 125
<210> 27
<211> 409
<212> DNA
<213> 大羊驼(Lama glama)
<220>
<221> CDS
<222> (1)..(408)
<400> 27
gaa gtc caa ctg caa gaa tca ggc gga ggc aca gtt caa cct ggc gga 48
Glu Val Gln Leu Gln Glu Ser Gly Gly Gly Thr Val Gln Pro Gly Gly
1 5 10 15
tca ctg aaa ctg tca tgc tca gca gca ccg gaa cgc gca ttt tca aat 96
Ser Leu Lys Leu Ser Cys Ser Ala Ala Pro Glu Arg Ala Phe Ser Asn
20 25 30
tat gca atg ggc tgg ttt aga caa gca ccg gga caa gaa aga gaa ttt 144
Tyr Ala Met Gly Trp Phe Arg Gln Ala Pro Gly Gln Glu Arg Glu Phe
35 40 45
gtt gca ggc att aca ggc tca ggc aga tca caa tat tac gca gat tca 192
Val Ala Gly Ile Thr Gly Ser Gly Arg Ser Gln Tyr Tyr Ala Asp Ser
50 55 60
gtc aaa gga cgc ttt aca att agc aga gat aat gca atg aat gcg gtc 240
Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Met Asn Ala Val
65 70 75 80
tac ctg caa atg aat tca gtt aaa gca gaa gat aca gcg gtc tat tat 288
Tyr Leu Gln Met Asn Ser Val Lys Ala Glu Asp Thr Ala Val Tyr Tyr
85 90 95
tgc gca gca aga gtt gtt ccg gtt ttt tca gat tca aca aaa ggc tat 336
Cys Ala Ala Arg Val Val Pro Val Phe Ser Asp Ser Thr Lys Gly Tyr
100 105 110
gtc tat tgg gga caa ggc aca caa gtt aca gtt tca tca cat cat cac 384
Val Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser His His His
115 120 125
cac cat cat gaa ccg gaa gca taa a 409
His His His Glu Pro Glu Ala
130 135
<210> 28
<211> 135
<212> PRT
<213> 大羊驼(Lama glama)
<400> 28
Glu Val Gln Leu Gln Glu Ser Gly Gly Gly Thr Val Gln Pro Gly Gly
1 5 10 15
Ser Leu Lys Leu Ser Cys Ser Ala Ala Pro Glu Arg Ala Phe Ser Asn
20 25 30
Tyr Ala Met Gly Trp Phe Arg Gln Ala Pro Gly Gln Glu Arg Glu Phe
35 40 45
Val Ala Gly Ile Thr Gly Ser Gly Arg Ser Gln Tyr Tyr Ala Asp Ser
50 55 60
Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Met Asn Ala Val
65 70 75 80
Tyr Leu Gln Met Asn Ser Val Lys Ala Glu Asp Thr Ala Val Tyr Tyr
85 90 95
Cys Ala Ala Arg Val Val Pro Val Phe Ser Asp Ser Thr Lys Gly Tyr
100 105 110
Val Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser His His His
115 120 125
His His His Glu Pro Glu Ala
130 135
<210> 29
<211> 1111
<212> DNA
<213> 大羊驼(Lama glama)
<220>
<221> CDS
<222> (1)..(1110)
<400> 29
gaa gtt caa ctg gtt gaa tca ggc gga ggc ctg gtt caa cct ggc gga 48
Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
1 5 10 15
tca ctg aga ctg tca tgc gca gca tca ggc ttt aca ttt tca gac tat 96
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Asp Tyr
20 25 30
tgg atg tat tgg gtc aga caa gca cct ggc aaa ggc ctg gaa tgg gtt 144
Trp Met Tyr Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
35 40 45
tca gaa att aac aca aat ggc ctg atc acg aaa tat ccg gat tca gtt 192
Ser Glu Ile Asn Thr Asn Gly Leu Ile Thr Lys Tyr Pro Asp Ser Val
50 55 60
aaa gga cgc ttt aca atc tca cgc gat aat gcg aaa aat acg ctg tat 240
Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Leu Tyr
65 70 75 80
ctg caa atg aat tca ctg cgt ccg gaa gat aca gca gtc tat tat tgc 288
Leu Gln Met Asn Ser Leu Arg Pro Glu Asp Thr Ala Val Tyr Tyr Cys
85 90 95
gca aga tca ccg agc ggc ttt aat aga ggc caa ggc aca ctg gtt aca 336
Ala Arg Ser Pro Ser Gly Phe Asn Arg Gly Gln Gly Thr Leu Val Thr
100 105 110
gtt tca tct ggc gga ggc gga tca ggc ggt ggc tca gaa gtc caa ctt 384
Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Ser Glu Val Gln Leu
115 120 125
gtc gaa agc gga ggt gga ctt gtt caa ccg gga aat tca ctt aga ctg 432
Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Asn Ser Leu Arg Leu
130 135 140
agc tgc gct gca agc gga ttt aca ttt agc tca ttt ggc atg tca tgg 480
Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Phe Gly Met Ser Trp
145 150 155 160
gtt cgc caa gcg cct gga aaa gga tta gaa tgg gtg tca tca att tca 528
Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val Ser Ser Ile Ser
165 170 175
ggc tca ggc agc gat aca ctg tat gca gat agc gtt aaa ggc aga ttt 576
Gly Ser Gly Ser Asp Thr Leu Tyr Ala Asp Ser Val Lys Gly Arg Phe
180 185 190
acg att agc aga gat aac gcg aaa aca aca ctg tac ctt cag atg aat 624
Thr Ile Ser Arg Asp Asn Ala Lys Thr Thr Leu Tyr Leu Gln Met Asn
195 200 205
agc ctt aga cct gaa gat acg gct gtt tat tac tgc aca att gga ggc 672
Ser Leu Arg Pro Glu Asp Thr Ala Val Tyr Tyr Cys Thr Ile Gly Gly
210 215 220
tca ctg tca aga tca tca cag gga aca ctt gtc aca gtt agc tca ggt 720
Ser Leu Ser Arg Ser Ser Gln Gly Thr Leu Val Thr Val Ser Ser Gly
225 230 235 240
ggc gga gga agc ggt ggc gga agc gaa gtt cag tta gtc gaa tct ggc 768
Gly Gly Gly Ser Gly Gly Gly Ser Glu Val Gln Leu Val Glu Ser Gly
245 250 255
ggt ggc ctt gtc cag cca ggc gga agc ctt cgc ctt tct tgc gca gcg 816
Gly Gly Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala
260 265 270
tct ggt ttt acg ttt agc gat tac tgg atg tac tgg gtg cgc cag gct 864
Ser Gly Phe Thr Phe Ser Asp Tyr Trp Met Tyr Trp Val Arg Gln Ala
275 280 285
cca ggc aaa gga ctt gag tgg gtt agc gaa atc aat aca aac ggg ctg 912
Pro Gly Lys Gly Leu Glu Trp Val Ser Glu Ile Asn Thr Asn Gly Leu
290 295 300
att aca aaa tac ccg gac tct gtc aaa ggc cgt ttc aca att tca aga 960
Ile Thr Lys Tyr Pro Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg
305 310 315 320
gac aac gcc aaa aac acc ctt tat tta caa atg aac agc ctg cgt cct 1008
Asp Asn Ala Lys Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Pro
325 330 335
gag gac aca gcg gtt tac tat tgc gcg aga agc ccg tca gga ttt aat 1056
Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg Ser Pro Ser Gly Phe Asn
340 345 350
cgc gga caa ggc aca tta gtg aca gtt agc agc cat cac cat cat cat 1104
Arg Gly Gln Gly Thr Leu Val Thr Val Ser Ser His His His His His
355 360 365
cat taa a 1111
His
<210> 30
<211> 369
<212> PRT
<213> 大羊驼(Lama glama)
<400> 30
Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
1 5 10 15
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Asp Tyr
20 25 30
Trp Met Tyr Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
35 40 45
Ser Glu Ile Asn Thr Asn Gly Leu Ile Thr Lys Tyr Pro Asp Ser Val
50 55 60
Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Leu Tyr
65 70 75 80
Leu Gln Met Asn Ser Leu Arg Pro Glu Asp Thr Ala Val Tyr Tyr Cys
85 90 95
Ala Arg Ser Pro Ser Gly Phe Asn Arg Gly Gln Gly Thr Leu Val Thr
100 105 110
Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Ser Glu Val Gln Leu
115 120 125
Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Asn Ser Leu Arg Leu
130 135 140
Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Phe Gly Met Ser Trp
145 150 155 160
Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val Ser Ser Ile Ser
165 170 175
Gly Ser Gly Ser Asp Thr Leu Tyr Ala Asp Ser Val Lys Gly Arg Phe
180 185 190
Thr Ile Ser Arg Asp Asn Ala Lys Thr Thr Leu Tyr Leu Gln Met Asn
195 200 205
Ser Leu Arg Pro Glu Asp Thr Ala Val Tyr Tyr Cys Thr Ile Gly Gly
210 215 220
Ser Leu Ser Arg Ser Ser Gln Gly Thr Leu Val Thr Val Ser Ser Gly
225 230 235 240
Gly Gly Gly Ser Gly Gly Gly Ser Glu Val Gln Leu Val Glu Ser Gly
245 250 255
Gly Gly Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala
260 265 270
Ser Gly Phe Thr Phe Ser Asp Tyr Trp Met Tyr Trp Val Arg Gln Ala
275 280 285
Pro Gly Lys Gly Leu Glu Trp Val Ser Glu Ile Asn Thr Asn Gly Leu
290 295 300
Ile Thr Lys Tyr Pro Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg
305 310 315 320
Asp Asn Ala Lys Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Pro
325 330 335
Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg Ser Pro Ser Gly Phe Asn
340 345 350
Arg Gly Gln Gly Thr Leu Val Thr Val Ser Ser His His His His His
355 360 365
His
<210> 31
<211> 799
<212> DNA
<213> 未知(Unknown)
<220>
<223> 骆驼科(Camelidae)
<220>
<221> CDS
<222> (1)..(798)
<400> 31
gaa gtt caa ctg gtt gaa tca ggc gga ggc ctg gtt caa cct ggc gga 48
Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
1 5 10 15
tca ctg aga ctg tca tgc gca gca tca ggc aga aca ttt tca tat aat 96
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Thr Phe Ser Tyr Asn
20 25 30
ccg atg ggc tgg ttt aga caa gca cct ggc aaa ggc aga gaa ctg gtt 144
Pro Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Gly Arg Glu Leu Val
35 40 45
gca gca att tca aga aca ggc gga tca aca tat tat ccg gat tca gtt 192
Ala Ala Ile Ser Arg Thr Gly Gly Ser Thr Tyr Tyr Pro Asp Ser Val
50 55 60
gaa gga cgc ttt aca att agc aga gat aat gcg aaa cgc atg gtc tat 240
Glu Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Arg Met Val Tyr
65 70 75 80
ctg caa atg aat tca ctg aga gca gaa gat aca gcg gtc tat tat tgc 288
Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys
85 90 95
gca gcg gca ggc gtt aga gcg gaa gat ggc aga gtt aga aca ctg ccg 336
Ala Ala Ala Gly Val Arg Ala Glu Asp Gly Arg Val Arg Thr Leu Pro
100 105 110
tca gaa tat aca ttt tgg gga caa ggc aca caa gtt aca gtt tca tca 384
Ser Glu Tyr Thr Phe Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser
115 120 125
gca gca gcg gaa gtc cag ctt gtc gaa agc gga ggc gga ctt gtc cag 432
Ala Ala Ala Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln
130 135 140
cct ggc gga agc ctt aga ctg agc tgc gct gca agc gga aga aca ttt 480
Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Thr Phe
145 150 155 160
agc tat aac cct atg gga tgg ttt cgc cag gct cct gga aaa gga cgc 528
Ser Tyr Asn Pro Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Gly Arg
165 170 175
gaa ctt gtc gca gcg att agc cgc aca ggc gga agc acg tat tat cct 576
Glu Leu Val Ala Ala Ile Ser Arg Thr Gly Gly Ser Thr Tyr Tyr Pro
180 185 190
gat agc gtt gaa ggc aga ttt aca atc tca cgc gat aac gca aaa aga 624
Asp Ser Val Glu Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Arg
195 200 205
atg gtg tat ctt cag atg aat agc ctt cgt gcg gaa gat acg gca gtt 672
Met Val Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val
210 215 220
tat tac tgt gcg gca gca ggc gtt cgc gca gag gat gga aga gtc cgc 720
Tyr Tyr Cys Ala Ala Ala Gly Val Arg Ala Glu Asp Gly Arg Val Arg
225 230 235 240
aca ctg ccg agc gaa tac acg ttc tgg gga cag gga aca cag gtc acg 768
Thr Leu Pro Ser Glu Tyr Thr Phe Trp Gly Gln Gly Thr Gln Val Thr
245 250 255
gtt agc tca cat cat cac cat cat cat taa a 799
Val Ser Ser His His His His His His
260 265
<210> 32
<211> 265
<212> PRT
<213> 未知(Unknown)
<220>
<223> 骆驼科(Camelidae)
<400> 32
Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
1 5 10 15
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Thr Phe Ser Tyr Asn
20 25 30
Pro Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Gly Arg Glu Leu Val
35 40 45
Ala Ala Ile Ser Arg Thr Gly Gly Ser Thr Tyr Tyr Pro Asp Ser Val
50 55 60
Glu Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Arg Met Val Tyr
65 70 75 80
Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys
85 90 95
Ala Ala Ala Gly Val Arg Ala Glu Asp Gly Arg Val Arg Thr Leu Pro
100 105 110
Ser Glu Tyr Thr Phe Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser
115 120 125
Ala Ala Ala Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln
130 135 140
Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Thr Phe
145 150 155 160
Ser Tyr Asn Pro Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Gly Arg
165 170 175
Glu Leu Val Ala Ala Ile Ser Arg Thr Gly Gly Ser Thr Tyr Tyr Pro
180 185 190
Asp Ser Val Glu Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Arg
195 200 205
Met Val Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val
210 215 220
Tyr Tyr Cys Ala Ala Ala Gly Val Arg Ala Glu Asp Gly Arg Val Arg
225 230 235 240
Thr Leu Pro Ser Glu Tyr Thr Phe Trp Gly Gln Gly Thr Gln Val Thr
245 250 255
Val Ser Ser His His His His His His
260 265
<210> 33
<211> 385
<212> DNA
<213> 单峰驼(Camelus dromedarius)
<220>
<221> CDS
<222> (1)..(384)
<400> 33
caa gtt caa ctg gtt gaa tca ggc gga ggc tca gtt caa gca ggc gga 48
Gln Val Gln Leu Val Glu Ser Gly Gly Gly Ser Val Gln Ala Gly Gly
1 5 10 15
tca ctg aga ctg tca tgc tca gca tca ggc tat aca tat att tca ggc 96
Ser Leu Arg Leu Ser Cys Ser Ala Ser Gly Tyr Thr Tyr Ile Ser Gly
20 25 30
tgg gtt aga caa gca cct ggc aaa ggc ctg gaa tgg gtt gca gca att 144
Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val Ala Ala Ile
35 40 45
aga tca tca gat ggc aca aca tat tat gcg gat tca gtc aaa gga cgc 192
Arg Ser Ser Asp Gly Thr Thr Tyr Tyr Ala Asp Ser Val Lys Gly Arg
50 55 60
ttt aca att tca cag gat aac gcg aaa aac acg gtc tat ctg caa atg 240
Phe Thr Ile Ser Gln Asp Asn Ala Lys Asn Thr Val Tyr Leu Gln Met
65 70 75 80
aat tca ctg aaa ccg gaa gat acg gcc atg tat tat tgc gca gca aca 288
Asn Ser Leu Lys Pro Glu Asp Thr Ala Met Tyr Tyr Cys Ala Ala Thr
85 90 95
gaa gtt gca ggc tgg ccg tta gat att ggc att tat gat tat tgg gga 336
Glu Val Ala Gly Trp Pro Leu Asp Ile Gly Ile Tyr Asp Tyr Trp Gly
100 105 110
caa ggc aca caa gtt aca gtt tca tca cat cat cac cat cac cac taa a 385
Gln Gly Thr Gln Val Thr Val Ser Ser His His His His His His
115 120 125
<210> 34
<211> 127
<212> PRT
<213> 单峰驼(Camelus dromedarius)
<400> 34
Gln Val Gln Leu Val Glu Ser Gly Gly Gly Ser Val Gln Ala Gly Gly
1 5 10 15
Ser Leu Arg Leu Ser Cys Ser Ala Ser Gly Tyr Thr Tyr Ile Ser Gly
20 25 30
Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val Ala Ala Ile
35 40 45
Arg Ser Ser Asp Gly Thr Thr Tyr Tyr Ala Asp Ser Val Lys Gly Arg
50 55 60
Phe Thr Ile Ser Gln Asp Asn Ala Lys Asn Thr Val Tyr Leu Gln Met
65 70 75 80
Asn Ser Leu Lys Pro Glu Asp Thr Ala Met Tyr Tyr Cys Ala Ala Thr
85 90 95
Glu Val Ala Gly Trp Pro Leu Asp Ile Gly Ile Tyr Asp Tyr Trp Gly
100 105 110
Gln Gly Thr Gln Val Thr Val Ser Ser His His His His His His
115 120 125
<210> 35
<211> 415
<212> DNA
<213> 单峰驼(Camelus dromedarius)
<220>
<221> CDS
<222> (1)..(414)
<400> 35
caa gtt caa ctg gtt gaa tca ggc gga ggc ctg gtt caa cct ggc gga 48
Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
1 5 10 15
tca ctg aga ctg tca tgc gca gca agc gga ggc tca gaa tat tca tat 96
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Gly Ser Glu Tyr Ser Tyr
20 25 30
tca aca ttt agc ctt ggc tgg ttt aga caa gca cct ggc caa ggc ctg 144
Ser Thr Phe Ser Leu Gly Trp Phe Arg Gln Ala Pro Gly Gln Gly Leu
35 40 45
gaa gca gtt gca gca att gca tca atg gga ggc ctg aca tat tat gca 192
Glu Ala Val Ala Ala Ile Ala Ser Met Gly Gly Leu Thr Tyr Tyr Ala
50 55 60
gat tca gtt aaa gga cgc ttt acg att agc cgt gat aac agc aaa aat 240
Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn
65 70 75 80
acg ctg tac ctg caa atg aat tca ctg aga gca gaa gat aca gcg gtc 288
Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val
85 90 95
tat tat tgc gca gca gtt aga ggc tat ttt atg aga ctg ccg tca tca 336
Tyr Tyr Cys Ala Ala Val Arg Gly Tyr Phe Met Arg Leu Pro Ser Ser
100 105 110
cat aat ttt cgc tat tgg gga caa ggc aca ctg gtt aca gtt tca tca 384
His Asn Phe Arg Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser
115 120 125
aga ggc aga cat cat cac cat cat cat taa a 415
Arg Gly Arg His His His His His His
130 135
<210> 36
<211> 137
<212> PRT
<213> 单峰驼(Camelus dromedarius)
<400> 36
Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
1 5 10 15
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Gly Ser Glu Tyr Ser Tyr
20 25 30
Ser Thr Phe Ser Leu Gly Trp Phe Arg Gln Ala Pro Gly Gln Gly Leu
35 40 45
Glu Ala Val Ala Ala Ile Ala Ser Met Gly Gly Leu Thr Tyr Tyr Ala
50 55 60
Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn
65 70 75 80
Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val
85 90 95
Tyr Tyr Cys Ala Ala Val Arg Gly Tyr Phe Met Arg Leu Pro Ser Ser
100 105 110
His Asn Phe Arg Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser
115 120 125
Arg Gly Arg His His His His His His
130 135
<210> 37
<211> 378
<212> DNA
<213> 单峰驼(Camelus dromedarius)
<220>
<221> CDS
<222> (1)..(378)
<400> 37
cag gtt cag ctt gtt gag tct gga ggt ggg ctt gta caa cca ggt ggc 48
Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
1 5 10 15
tct ttg cgc tta agc tgt gct gca tct ggc ttt acg ttc tcg aat gat 96
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Asn Asp
20 25 30
gcc atg aca tgg gta cgt caa gca cct gga aaa gct ctg gaa tgg gtg 144
Ala Met Thr Trp Val Arg Gln Ala Pro Gly Lys Ala Leu Glu Trp Val
35 40 45
tca tcc att acc ctc tta gga gga gga att ccg tat tac agc gac aca 192
Ser Ser Ile Thr Leu Leu Gly Gly Gly Ile Pro Tyr Tyr Ser Asp Thr
50 55 60
gtt aaa ggc cgg ttt acc atc tca agg gat aac acg aag aac atg ctg 240
Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Thr Lys Asn Met Leu
65 70 75 80
tac ttg cag atg aat agc ctg aaa ccg gaa gat aca gcg gtg tat tat 288
Tyr Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr
85 90 95
tgc gcg aaa ggc ttt aag tcc gac tat ccc aga ggt caa ggg aca caa 336
Cys Ala Lys Gly Phe Lys Ser Asp Tyr Pro Arg Gly Gln Gly Thr Gln
100 105 110
gtc act gtc agt tca cga ggc aga cat cac cac cat cat cat 378
Val Thr Val Ser Ser Arg Gly Arg His His His His His His
115 120 125
<210> 38
<211> 126
<212> PRT
<213> 单峰驼(Camelus dromedarius)
<400> 38
Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
1 5 10 15
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Asn Asp
20 25 30
Ala Met Thr Trp Val Arg Gln Ala Pro Gly Lys Ala Leu Glu Trp Val
35 40 45
Ser Ser Ile Thr Leu Leu Gly Gly Gly Ile Pro Tyr Tyr Ser Asp Thr
50 55 60
Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Thr Lys Asn Met Leu
65 70 75 80
Tyr Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr
85 90 95
Cys Ala Lys Gly Phe Lys Ser Asp Tyr Pro Arg Gly Gln Gly Thr Gln
100 105 110
Val Thr Val Ser Ser Arg Gly Arg His His His His His His
115 120 125
<210> 39
<211> 575
<212> DNA
<213> 芽孢杆菌(Bacillus sp.)KSM-S237
<220>
<223> KSM-S237启动子
<400> 39
gatttgccga tgcaacaggc ttatatttag aggaaatttc tttttaaatt gaatacggaa 60
taaaatcagg taaacaggtc ctgattttat ttttttgagt tttttagaga actgaagatt 120
gaaataaaag tagaagacaa aggacataag aaaattgcat tagttttaat tatagaaaac 180
gcctttttat aattatttat acctagaacg aaaatactgt ttcgaaagcg gtttactata 240
aaaccttata ttccggctct tttttaaaac agggggtaaa aattcactct agtattctaa 300
tttcaacatg ctataataaa tttgtaagac gcaatatgca tctctttttt tacgatatat 360
gtaagcggtt aaccttgtgc tatatgccga tttaggaagg ggggtagatt gagtcaagta 420
gtaataatat agataactta taagttgttg agaagcagga gagcatctgg gttactcaca 480
agttttttta aaactttaac gaaagcactt tcggtaatgc ttatgaattt agctatttga 540
ttcaattact ttaaaaatat ttaggaggta atatg 575
<210> 40
<211> 617
<212> DNA
<213> 芽孢杆菌(Bacillus sp.)KSM-64
<220>
<223> KSM-64启动子
<400> 40
gaacaagtac ttaccatttt agagtcaaaa gatagaagcc aagcaggatt tgccgatgca 60
accggcttat atttagaggg aatttctttt taaattgaat acggaataaa atcaggtaaa 120
caggtcctga ttttattttt ttgaattttt ttgagaacta aagattgaaa tagaagtaga 180
agacaacgga cataagaaaa ttgtattagt tttaattata gaaaacgctt ttctataatt 240
atttatacct agaacgaaaa tactgtttcg aaagcggttt actataaaac cttatattcc 300
ggctcttttt ttaaacaggg ggtgaaaatt cactctagta ttctaatttc aacatgctat 360
aataaatttg taagacgcaa tatacatctt ttttttatga tatttgtaag cggttaacct 420
tgtgctatat gccgatttag gaagggggta gattgagtca agtagtcata atttagataa 480
cttataagtt gttgagaagc aggagagaat ctgggttact cacaagtttt ttaaaacatt 540
atcgaaagca ctttcggtta tgcttatgaa tttagctatt tgattcaatt actttaataa 600
ttttaggagg taatatg 617
<210> 41
<211> 193
<212> DNA
<213> 枯草杆菌(Bacillus subtilis)
<220>
<223> spoVG启动子
<400> 41
taagaaaagt gattctggga gagccgggat cactttttta tttaccttat gcccgaaatg 60
aaagctttat gacctaattg tgtaactata tcctattttt tcaaaaaata ttttaaaaac 120
gagcaggatt tcagaaaaaa tcgtggaatt gatacactaa tgcttttata tagggaaaag 180
gtggtgaact act 193
<210> 42
<211> 87
<212> DNA
<213> 芽孢杆菌(Bacillus sp.)KSM-S237
<220>
<223> KSM-S237信号
<400> 42
atgttaagaa agaaaacaaa gcagttgatt tcttccattc ttattttagt tttacttcta 60
tctttatttc cggcagctct tgcagca 87
<210> 43
<211> 87
<212> DNA
<213> 芽孢杆菌(Bacillus sp.)KSM-64
<220>
<223> KSM-64信号
<400> 43
atgttaagaa agaaaacaaa gcagttgatt tcttccattc ttattttagt tttacttcta 60
tctttatttc cgacagctct tgcagca 87
<210> 44
<211> 74
<212> DNA
<213> 枯草杆菌(Bacillus subtilis)
<220>
<223> amyE信号
<400> 44
atgtttgcaa aacgattcaa aacctcttta ctgccgttat tcgctggatt tttattgctg 60
tttcatttgg ttct 74
<210> 45
<211> 15
<212> DNA
<213> 人工
<220>
<223> 连接顺序
<400> 45
gcagctcttg cagca 15
<210> 46
<211> 15
<212> DNA
<213> 人工
<220>
<223> 连接顺序
<400> 46
atagggttat ctaaa 15
<210> 47
<211> 25
<212> DNA
<213> 人工
<220>
<223> 引物
<400> 47
tgctgcaaga gctgccggaa ataaa 25
<210> 48
<211> 21
<212> DNA
<213> 人工
<220>
<223> 引物
<400> 48
tctattaaac tagttatagg g 21
<210> 49
<211> 31
<212> DNA
<213> 人工
<220>
<223> 引物
<400> 49
gatccccggg aattcctgtt ataaaaaaag g 31
<210> 50
<211> 27
<212> DNA
<213> 人工
<220>
<223> 引物
<400> 50
atgatgttaa gaaagaaaac aaagcag 27
<210> 51
<211> 38
<212> DNA
<213> 人工
<220>
<223> 引物
<400> 51
gaattcccgg ggatctaaga aaagtgattc tgggagag 38
<210> 52
<211> 36
<212> DNA
<213> 人工
<220>
<223> 引物
<400> 52
ctttcttaac atcatagtag ttcaccacct tttccc 36
<210> 53
<211> 35
<212> DNA
<213> 人工
<220>
<223> 引物
<400> 53
ccggcgatgg ccatggcaga tgttcaactg caaga 35
<210> 54
<211> 35
<212> DNA
<213> 人工
<220>
<223> 引物
<400> 54
gtgcggccgc aagctttaat gatggtggtg atgat 35
<210> 55
<211> 35
<212> DNA
<213> 人工
<220>
<223> 引物
<400> 55
ccggcgatgg ccatgcaagt ccaactgcaa gaatc 35
<210> 56
<211> 35
<212> DNA
<213> 人工
<220>
<223> 引物
<400> 56
gtgcggccgc aagctttaat gatgatggtg atgat 35
<210> 57
<211> 35
<212> DNA
<213> 人工
<220>
<223> 引物r
<400> 57
ccggcgatgg ccatgcaagt tcaactggtt gaatc 35
<210> 58
<211> 35
<212> DNA
<213> 人工
<220>
<223> 引物
<400> 58
gtgcggccgc aagctttagt ggtgatggtg atgat 35
<210> 59
<211> 35
<212> DNA
<213> 人工
<220>
<223> 引物
<400> 59
ccggcgatgg ccatgcaagt ccaactgcaa gaatc 35
<210> 60
<211> 35
<212> DNA
<213> 人工
<220>
<223> 引物
<400> 60
gtgcggccgc aagctttagt ggtgatggtg atgat 35
<210> 61
<211> 35
<212> DNA
<213> 人工
<220>
<223> 引物
<400> 61
ccggcgatgg ccatgcaagt ccaactgcaa gaatc 35
<210> 62
<211> 35
<212> DNA
<213> 人工
<220>
<223> 引物
<400> 62
gtgcggccgc aagctttaat gatgatgatg gtgat 35
<210> 63
<211> 399
<212> DNA
<213> 未知(Unknown)
<220>
<223> 未知物种(Unknown species)
<220>
<221> CDS
<222> (1)..(399)
<400> 63
caa gtc caa ctg caa gaa tca ggc gga ggc ctg gtt caa gca ggc gga 48
Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Gly
1 5 10 15
tca ctg aga ctg tca tgc gca gtt tca ggc aga aca ttt tca aac tat 96
Ser Leu Arg Leu Ser Cys Ala Val Ser Gly Arg Thr Phe Ser Asn Tyr
20 25 30
tat tca ggc tgg ttt aga cag gca ccg gga aaa gaa aga gaa ttt ctg 144
Tyr Ser Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Phe Leu
35 40 45
gca tca att cgc tgg tca gat agc aca aca aat tat gca gat agc gtc 192
Ala Ser Ile Arg Trp Ser Asp Ser Thr Thr Asn Tyr Ala Asp Ser Val
50 55 60
aaa gga cgc ttt aca att tca aga gat aca gcg aaa aac acg gtc tac 240
Lys Gly Arg Phe Thr Ile Ser Arg Asp Thr Ala Lys Asn Thr Val Tyr
65 70 75 80
ctg caa atg aat tca ctg aaa ctg gaa gat acg gca gtt tat cat tgc 288
Leu Gln Met Asn Ser Leu Lys Leu Glu Asp Thr Ala Val Tyr His Cys
85 90 95
gca gca aga cgc ctg gca aca tat gat tat tgg gga caa ggc aca caa 336
Ala Ala Arg Arg Leu Ala Thr Tyr Asp Tyr Trp Gly Gln Gly Thr Gln
100 105 110
gtt aca gtt tca tca cat cat cac cat cac cac taa 372
Val Thr Val Ser Ser His His His His His His
115 120
<210> 64
<211> 123
<212> PRT
<213> 未知(Unknown)
<220>
<223> 未知物种(Unknown species)
<400> 64
Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Gly
1 5 10 15
Ser Leu Arg Leu Ser Cys Ala Val Ser Gly Arg Thr Phe Ser Asn Tyr
20 25 30
Tyr Ser Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Phe Leu
35 40 45
Ala Ser Ile Arg Trp Ser Asp Ser Thr Thr Asn Tyr Ala Asp Ser Val
50 55 60
Lys Gly Arg Phe Thr Ile Ser Arg Asp Thr Ala Lys Asn Thr Val Tyr
65 70 75 80
Leu Gln Met Asn Ser Leu Lys Leu Glu Asp Thr Ala Val Tyr His Cys
85 90 95
Ala Ala Arg Arg Leu Ala Thr Tyr Asp Tyr Trp Gly Gln Gly Thr Gln
100 105 110
Val Thr Val Ser Ser His His His His His His
115 120
<210> 65
<211> 399
<212> DNA
<213> 未知(Unknown)
<220>
<223> 未知物种(Unknown species)
<220>
<221> CDS
<222> (1)..(399)
<400> 65
caa gtc caa ctg caa gaa tca ggc gga ggc ctg gtt atg aca ggc gga 48
Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Met Thr Gly Gly
1 5 10 15
tca ctg aga ctg tca tgc gca gtt tca ggc aga aca att gat gtt tca 96
Ser Leu Arg Leu Ser Cys Ala Val Ser Gly Arg Thr Ile Asp Val Ser
20 25 30
gtt atg gcg tgg ttt aga caa gca ccg gga aaa gaa aga gaa ttt gtt 144
Val Met Ala Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Phe Val
35 40 45
agc ggc atg aga tgg tca ggc atg aca aca tat tca gca gat agc gtt 192
Ser Gly Met Arg Trp Ser Gly Met Thr Thr Tyr Ser Ala Asp Ser Val
50 55 60
aaa gac cgc ttt aca att tca cgc gac aaa aca aaa aac acg gtc tac 240
Lys Asp Arg Phe Thr Ile Ser Arg Asp Lys Thr Lys Asn Thr Val Tyr
65 70 75 80
ctg caa atg aat tca ctg aaa ccg gaa gat aca gcg gtc tat tat tgc 288
Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys
85 90 95
gca gca aga agc aga ttt att gtt ggc gtt ccg caa gca aga gat ctg 336
Ala Ala Arg Ser Arg Phe Ile Val Gly Val Pro Gln Ala Arg Asp Leu
100 105 110
tat gat tat tgg gga caa ggc aca caa gtt aca gtt tca tca cat cat 384
Tyr Asp Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser His His
115 120 125
cac cat cac cac taa 399
His His His His
130
<210> 66
<211> 132
<212> PRT
<213> 未知(Unknown)
<220>
<223> 未知物种(Unknown species)
<400> 66
Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Met Thr Gly Gly
1 5 10 15
Ser Leu Arg Leu Ser Cys Ala Val Ser Gly Arg Thr Ile Asp Val Ser
20 25 30
Val Met Ala Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Phe Val
35 40 45
Ser Gly Met Arg Trp Ser Gly Met Thr Thr Tyr Ser Ala Asp Ser Val
50 55 60
Lys Asp Arg Phe Thr Ile Ser Arg Asp Lys Thr Lys Asn Thr Val Tyr
65 70 75 80
Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys
85 90 95
Ala Ala Arg Ser Arg Phe Ile Val Gly Val Pro Gln Ala Arg Asp Leu
100 105 110
Tyr Asp Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser His His
115 120 125
His His His His
130
<210> 67
<211> 384
<212> DNA
<213> 未知(Unknown)
<220>
<223> 未知物种(Unknown species)
<220>
<221> CDS
<222> (1)..(384)
<400> 67
caa gtc caa ctg caa gaa tca ggc gga ggc ctg gtt caa gca ggc gga 48
Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Gly
1 5 10 15
tca ctg aga ctg tca tgc gca gca tca ggc aga atg ttt tca att aat 96
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Met Phe Ser Ile Asn
20 25 30
tca atg ggc tgg tat aga cag gca ccg gga aaa gaa aga gaa ctg gtt 144
Ser Met Gly Trp Tyr Arg Gln Ala Pro Gly Lys Glu Arg Glu Leu Val
35 40 45
gca aca att tca gaa gca ggc aca aca aca tat gca gat tca gtt aga 192
Ala Thr Ile Ser Glu Ala Gly Thr Thr Thr Tyr Ala Asp Ser Val Arg
50 55 60
gga cgc ttt aca att gca cgc gat aat gca aaa aac acg gtc tat ctg 240
Gly Arg Phe Thr Ile Ala Arg Asp Asn Ala Lys Asn Thr Val Tyr Leu
65 70 75 80
caa atg aat tca ctg aat ccg gaa gat aca gcg gtc tat tat tgc aat 288
Gln Met Asn Ser Leu Asn Pro Glu Asp Thr Ala Val Tyr Tyr Cys Asn
85 90 95
gca tat att cag ctg gat agc aca att tgg ttt aga gca tat tgg gga 336
Ala Tyr Ile Gln Leu Asp Ser Thr Ile Trp Phe Arg Ala Tyr Trp Gly
100 105 110
caa ggc aca caa gtt aca gtt tca tca cat cat cac cat cac cac taa 384
Gln Gly Thr Gln Val Thr Val Ser Ser His His His His His His
115 120 125
<210> 68
<211> 127
<212> PRT
<213> 未知(Unknown)
<220>
<223> 未知物种(Unknown species)
<400> 68
Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Gly
1 5 10 15
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Met Phe Ser Ile Asn
20 25 30
Ser Met Gly Trp Tyr Arg Gln Ala Pro Gly Lys Glu Arg Glu Leu Val
35 40 45
Ala Thr Ile Ser Glu Ala Gly Thr Thr Thr Tyr Ala Asp Ser Val Arg
50 55 60
Gly Arg Phe Thr Ile Ala Arg Asp Asn Ala Lys Asn Thr Val Tyr Leu
65 70 75 80
Gln Met Asn Ser Leu Asn Pro Glu Asp Thr Ala Val Tyr Tyr Cys Asn
85 90 95
Ala Tyr Ile Gln Leu Asp Ser Thr Ile Trp Phe Arg Ala Tyr Trp Gly
100 105 110
Gln Gly Thr Gln Val Thr Val Ser Ser His His His His His His
115 120 125
<210> 69
<211> 393
<212> DNA
<213> 未知(Unknown)
<220>
<223> 未知物种(Unknown species)
<220>
<221> CDS
<222> (1)..(393)
<400> 69
caa gtc caa ctg caa gaa tca ggc gga ggc ctg gtt caa cct ggc gga 48
Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
1 5 10 15
tca ctg aga ctg ttt tgc gca gca tca ggc ttt aca ttt tca agc tat 96
Ser Leu Arg Leu Phe Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
20 25 30
gca atg aga tgg tat aga caa gca ccg gga aaa gaa aga gaa ctg gtt 144
Ala Met Arg Trp Tyr Arg Gln Ala Pro Gly Lys Glu Arg Glu Leu Val
35 40 45
gca gca att aca tca gca ggc gga tca aca cat tat gca gat tca gtc 192
Ala Ala Ile Thr Ser Ala Gly Gly Ser Thr His Tyr Ala Asp Ser Val
50 55 60
aaa gaa cgc ttt aca atc agc aga gat aac gcg aaa aac acg atg tat 240
Lys Glu Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Met Tyr
65 70 75 80
ctg caa atg aat tca ctg aaa ccg gaa gat aca gcg gtc tat tat tgc 288
Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys
85 90 95
aat gcg aga aga gat tat ggc gat tca tgg ttt aca gct ggc gga ggc 336
Asn Ala Arg Arg Asp Tyr Gly Asp Ser Trp Phe Thr Ala Gly Gly Gly
100 105 110
tat tgg gga caa ggc aca caa gtt aca gtt tca tca cat cat cac cat 384
Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser His His His His
115 120 125
cac cac taa 393
His His
130
<210> 70
<211> 130
<212> PRT
<213> 未知(Unknown)
<220>
<223> 未知物种(Unknown species)
<400> 70
Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
1 5 10 15
Ser Leu Arg Leu Phe Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
20 25 30
Ala Met Arg Trp Tyr Arg Gln Ala Pro Gly Lys Glu Arg Glu Leu Val
35 40 45
Ala Ala Ile Thr Ser Ala Gly Gly Ser Thr His Tyr Ala Asp Ser Val
50 55 60
Lys Glu Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Met Tyr
65 70 75 80
Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys
85 90 95
Asn Ala Arg Arg Asp Tyr Gly Asp Ser Trp Phe Thr Ala Gly Gly Gly
100 105 110
Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser His His His His
115 120 125
His His
130
<210> 71
<211> 378
<212> DNA
<213> 未知(Unknown)
<220>
<223> 未知物种(Unknown species)
<220>
<221> CDS
<222> (1)..(378)
<400> 71
caa gtc caa ctg caa gaa tca ggc gga ggc ctg gtt caa agc gga ggc 48
Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Ser Gly Gly
1 5 10 15
tca ctg aga ctg tca tgc gca gca tca aga aac att aat agc atg cat 96
Ser Leu Arg Leu Ser Cys Ala Ala Ser Arg Asn Ile Asn Ser Met His
20 25 30
gtt gtc ggc tgg tat aga caa gca ccg gga aat caa aga gaa ctg gtt 144
Val Val Gly Trp Tyr Arg Gln Ala Pro Gly Asn Gln Arg Glu Leu Val
35 40 45
gca tca att aca gat gat ggc agc aca gat tat gtc gat tca gtt aaa 192
Ala Ser Ile Thr Asp Asp Gly Ser Thr Asp Tyr Val Asp Ser Val Lys
50 55 60
gga cgc ttt aca atc agc aga gat att gcg gaa aat acg gtc tat ctg 240
Gly Arg Phe Thr Ile Ser Arg Asp Ile Ala Glu Asn Thr Val Tyr Leu
65 70 75 80
caa atg aat tca ctg aac ccg gaa gat aca gca gtc tat tat tgc aaa 288
Gln Met Asn Ser Leu Asn Pro Glu Asp Thr Ala Val Tyr Tyr Cys Lys
85 90 95
ggc aca atc gtc gtt ttt aca acg ccg atg cat tat tgg gga aaa ggc 336
Gly Thr Ile Val Val Phe Thr Thr Pro Met His Tyr Trp Gly Lys Gly
100 105 110
aca caa gtt aca gtt tca tca cat cat cac cat cac cac taa 378
Thr Gln Val Thr Val Ser Ser His His His His His His
115 120 125
<210> 72
<211> 125
<212> PRT
<213> 未知(Unknown)
<220>
<223> 未知物种(Unknown species)
<400> 72
Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Ser Gly Gly
1 5 10 15
Ser Leu Arg Leu Ser Cys Ala Ala Ser Arg Asn Ile Asn Ser Met His
20 25 30
Val Val Gly Trp Tyr Arg Gln Ala Pro Gly Asn Gln Arg Glu Leu Val
35 40 45
Ala Ser Ile Thr Asp Asp Gly Ser Thr Asp Tyr Val Asp Ser Val Lys
50 55 60
Gly Arg Phe Thr Ile Ser Arg Asp Ile Ala Glu Asn Thr Val Tyr Leu
65 70 75 80
Gln Met Asn Ser Leu Asn Pro Glu Asp Thr Ala Val Tyr Tyr Cys Lys
85 90 95
Gly Thr Ile Val Val Phe Thr Thr Pro Met His Tyr Trp Gly Lys Gly
100 105 110
Thr Gln Val Thr Val Ser Ser His His His His His His
115 120 125
<210> 73
<211> 366
<212> DNA
<213> 未知(Unknown)
<220>
<223> 未知物种(Unknown species)
<220>
<221> CDS
<222> (1)..(366)
<400> 73
caa gtc caa ctg caa gaa tca ggc gga ggc ctg gtt caa cct ggc gga 48
Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
1 5 10 15
tca ctg aga ctg tca tgc aca gca ccg aga att atc ttt ttt atg tat 96
Ser Leu Arg Leu Ser Cys Thr Ala Pro Arg Ile Ile Phe Phe Met Tyr
20 25 30
gac gtc ggc tgg tat aga caa gca ccg gaa aaa caa aga gaa ctg gtt 144
Asp Val Gly Trp Tyr Arg Gln Ala Pro Glu Lys Gln Arg Glu Leu Val
35 40 45
gca cag att aac tca gat gtc agc aca aaa tat gcg gat tca gtc aaa 192
Ala Gln Ile Asn Ser Asp Val Ser Thr Lys Tyr Ala Asp Ser Val Lys
50 55 60
gga cgc ttt aca att tca cgc gat aat gcg aaa aga acg gtc tat ctg 240
Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Arg Thr Val Tyr Leu
65 70 75 80
caa atg aat gat ctg aaa ccg gaa gat gca gca gtc tat tat tgc aat 288
Gln Met Asn Asp Leu Lys Pro Glu Asp Ala Ala Val Tyr Tyr Cys Asn
85 90 95
gtt aga cgc gca tca gca gat tat tgg gga caa ggc aca caa gtt aca 336
Val Arg Arg Ala Ser Ala Asp Tyr Trp Gly Gln Gly Thr Gln Val Thr
100 105 110
gtt tca tca cat cat cac cat cac cac taa 366
Val Ser Ser His His His His His His
115 120
<210> 74
<211> 121
<212> PRT
<213> 未知(Unknown)
<220>
<223> 未知物种(Unknown species)
<400> 74
Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
1 5 10 15
Ser Leu Arg Leu Ser Cys Thr Ala Pro Arg Ile Ile Phe Phe Met Tyr
20 25 30
Asp Val Gly Trp Tyr Arg Gln Ala Pro Glu Lys Gln Arg Glu Leu Val
35 40 45
Ala Gln Ile Asn Ser Asp Val Ser Thr Lys Tyr Ala Asp Ser Val Lys
50 55 60
Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Arg Thr Val Tyr Leu
65 70 75 80
Gln Met Asn Asp Leu Lys Pro Glu Asp Ala Ala Val Tyr Tyr Cys Asn
85 90 95
Val Arg Arg Ala Ser Ala Asp Tyr Trp Gly Gln Gly Thr Gln Val Thr
100 105 110
Val Ser Ser His His His His His His
115 120
<210> 75
<211> 360
<212> DNA
<213> 未知(Unknown)
<220>
<223> 未知物种(Unknown species)
<220>
<221> CDS
<222> (1)..(360)
<400> 75
caa gtc caa ctg caa gaa tca ggc gga ggc ctg gtt caa cct ggc gga 48
Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
1 5 10 15
tca ctg aga ctg tca tgc gca gca tca ggc aca att ttt tca cgc aat 96
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Thr Ile Phe Ser Arg Asn
20 25 30
att atg ggc tgg tat aga caa gca ccg gga aaa gaa aga gaa ctg gtt 144
Ile Met Gly Trp Tyr Arg Gln Ala Pro Gly Lys Glu Arg Glu Leu Val
35 40 45
gca tca att tat agc gat cgc agc aca tgg tat gca gaa tca gtt gaa 192
Ala Ser Ile Tyr Ser Asp Arg Ser Thr Trp Tyr Ala Glu Ser Val Glu
50 55 60
ggc aga ttt aca atc agc cgt gat aac gtc aaa aac aca ctg tat ctg 240
Gly Arg Phe Thr Ile Ser Arg Asp Asn Val Lys Asn Thr Leu Tyr Leu
65 70 75 80
caa atg aat agc ctg aaa ccg gaa gat acg gca atg tat tat tgc aga 288
Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Met Tyr Tyr Cys Arg
85 90 95
gat aga aca ctt ggc tca tgg gga caa ggc aca caa gtt aca gtt tca 336
Asp Arg Thr Leu Gly Ser Trp Gly Gln Gly Thr Gln Val Thr Val Ser
100 105 110
tca cat cat cac cat cac cac taa 360
Ser His His His His His His
115
<210> 76
<211> 119
<212> PRT
<213> 未知(Unknown)
<220>
<223> 未知物种(Unknown species)
<400> 76
Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
1 5 10 15
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Thr Ile Phe Ser Arg Asn
20 25 30
Ile Met Gly Trp Tyr Arg Gln Ala Pro Gly Lys Glu Arg Glu Leu Val
35 40 45
Ala Ser Ile Tyr Ser Asp Arg Ser Thr Trp Tyr Ala Glu Ser Val Glu
50 55 60
Gly Arg Phe Thr Ile Ser Arg Asp Asn Val Lys Asn Thr Leu Tyr Leu
65 70 75 80
Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Met Tyr Tyr Cys Arg
85 90 95
Asp Arg Thr Leu Gly Ser Trp Gly Gln Gly Thr Gln Val Thr Val Ser
100 105 110
Ser His His His His His His
115
<210> 77
<211> 426
<212> DNA
<213> 未知(Unknown)
<220>
<223> 未知物种(Unknown species)
<220>
<221> CDS
<222> (1)..(426)
<400> 77
caa gtc caa ctg caa gaa tca ggc gga ggc ctg gtt caa cct ggc gga 48
Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
1 5 10 15
tca ctg aga ctg tca tgc gca gca tca ggc ttt aca ctg ggc tat tat 96
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Leu Gly Tyr Tyr
20 25 30
ccg att ggc tgg ttt aga caa gca cct ggc aaa ggc ctg gaa ggc gtt 144
Pro Ile Gly Trp Phe Arg Gln Ala Pro Gly Lys Gly Leu Glu Gly Val
35 40 45
tca tgc att tca ggc tca ggc gga tca gca aat tat gca gca agc gtt 192
Ser Cys Ile Ser Gly Ser Gly Gly Ser Ala Asn Tyr Ala Ala Ser Val
50 55 60
aaa gga cgc ttt aca att tca cgc gat aac gcg aaa aat acg gtc tat 240
Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Val Tyr
65 70 75 80
ctg caa atg aat tca ctg aaa ccg gaa gat acg gcg atc tat tat tgc 288
Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Ile Tyr Tyr Cys
85 90 95
gca gca gat ctg tca tca ctg aca aca gtt caa gca atg tgc gtt att 336
Ala Ala Asp Leu Ser Ser Leu Thr Thr Val Gln Ala Met Cys Val Ile
100 105 110
ccg cgt ccg gga ttt tca gca aaa gca tat gat tat tgg gga ctg ggc 384
Pro Arg Pro Gly Phe Ser Ala Lys Ala Tyr Asp Tyr Trp Gly Leu Gly
115 120 125
aca caa gtt aca gtt tca tca cat cat cac cat cac cac taa 426
Thr Gln Val Thr Val Ser Ser His His His His His His
130 135 140
<210> 78
<211> 141
<212> PRT
<213> 未知(Unknown)
<220>
<223> 未知物种(Unknown species)
<400> 78
Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
1 5 10 15
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Leu Gly Tyr Tyr
20 25 30
Pro Ile Gly Trp Phe Arg Gln Ala Pro Gly Lys Gly Leu Glu Gly Val
35 40 45
Ser Cys Ile Ser Gly Ser Gly Gly Ser Ala Asn Tyr Ala Ala Ser Val
50 55 60
Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Val Tyr
65 70 75 80
Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Ile Tyr Tyr Cys
85 90 95
Ala Ala Asp Leu Ser Ser Leu Thr Thr Val Gln Ala Met Cys Val Ile
100 105 110
Pro Arg Pro Gly Phe Ser Ala Lys Ala Tyr Asp Tyr Trp Gly Leu Gly
115 120 125
Thr Gln Val Thr Val Ser Ser His His His His His His
130 135 140
<210> 79
<211> 381
<212> DNA
<213> 未知(Unknown)
<220>
<223> 未知物种(Unknown species)
<220>
<221> CDS
<222> (1)..(381)
<400> 79
caa gtc caa ctg caa gaa tca ggc gga ggc ctg gtt caa cct ggc gga 48
Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
1 5 10 15
tca ctg aga ctg tca tgc gca gca tca ggc tca gtt tca aga aca tat 96
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Ser Val Ser Arg Thr Tyr
20 25 30
gtc atg ggc tgg tat aga caa aca ccg gga aat caa aga gaa ctg gtt 144
Val Met Gly Trp Tyr Arg Gln Thr Pro Gly Asn Gln Arg Glu Leu Val
35 40 45
gca aca att aca tca gtt ggc agc aca aat tat gcg gat tca ctg aaa 192
Ala Thr Ile Thr Ser Val Gly Ser Thr Asn Tyr Ala Asp Ser Leu Lys
50 55 60
ggc cgt ttt aca att tca cgc gaa aat gcg gaa aac aca gtc tat ctg 240
Gly Arg Phe Thr Ile Ser Arg Glu Asn Ala Glu Asn Thr Val Tyr Leu
65 70 75 80
caa atg aat tca ctc aaa ccg gaa gat acg gcg atc tat tat tgc aaa 288
Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Ile Tyr Tyr Cys Lys
85 90 95
tat atc cgc tat agc ccg att cat gca ccg ctg gat tat tgg gga caa 336
Tyr Ile Arg Tyr Ser Pro Ile His Ala Pro Leu Asp Tyr Trp Gly Gln
100 105 110
ggc aca caa gtt aca gtt tca tca cat cat cac cat cac cac taa 381
Gly Thr Gln Val Thr Val Ser Ser His His His His His His
115 120 125
<210> 80
<211> 126
<212> PRT
<213> 未知(Unknown)
<220>
<223> 未知物种(Unknown species)
<400> 80
Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
1 5 10 15
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Ser Val Ser Arg Thr Tyr
20 25 30
Val Met Gly Trp Tyr Arg Gln Thr Pro Gly Asn Gln Arg Glu Leu Val
35 40 45
Ala Thr Ile Thr Ser Val Gly Ser Thr Asn Tyr Ala Asp Ser Leu Lys
50 55 60
Gly Arg Phe Thr Ile Ser Arg Glu Asn Ala Glu Asn Thr Val Tyr Leu
65 70 75 80
Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Ile Tyr Tyr Cys Lys
85 90 95
Tyr Ile Arg Tyr Ser Pro Ile His Ala Pro Leu Asp Tyr Trp Gly Gln
100 105 110
Gly Thr Gln Val Thr Val Ser Ser His His His His His His
115 120 125
<210> 81
<211> 381
<212> DNA
<213> 未知(Unknown)
<220>
<223> 未知物种(Unknown species)
<220>
<221> CDS
<222> (1)..(381)
<400> 81
caa gtc caa ctg caa gaa tca ggc gga ggc ctg gtt caa cct ggc gga 48
Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
1 5 10 15
tca ctg aga ctg tca tgc gca gca tca ggc tca att ttt tca att tat 96
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Ser Ile Phe Ser Ile Tyr
20 25 30
gca atg ggc tgg tat aga caa gca ccg gga aaa caa aga gaa ctg gtt 144
Ala Met Gly Trp Tyr Arg Gln Ala Pro Gly Lys Gln Arg Glu Leu Val
35 40 45
gca tca att tca tct ggc gga ggc aca aat tat gca gat tca gtt aaa 192
Ala Ser Ile Ser Ser Gly Gly Gly Thr Asn Tyr Ala Asp Ser Val Lys
50 55 60
gga cgc ttt acg att tca ggc gat aat gcg aaa aat acg gtc tac ctg 240
Gly Arg Phe Thr Ile Ser Gly Asp Asn Ala Lys Asn Thr Val Tyr Leu
65 70 75 80
caa atg aat tca ctg aaa ccg gaa gat aca gcg gtc tat tat tgc aaa 288
Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys Lys
85 90 95
aga gaa gat tac tca gca tat gca ccg cct tca ggc agc aga ggc aga 336
Arg Glu Asp Tyr Ser Ala Tyr Ala Pro Pro Ser Gly Ser Arg Gly Arg
100 105 110
ggc aca caa gtt aca gtt tca tca cat cat cac cat cac cac taa 381
Gly Thr Gln Val Thr Val Ser Ser His His His His His His
115 120 125
<210> 82
<211> 126
<212> PRT
<213> 未知(Unknown)
<220>
<223> 未知物种(Unknown species)
<400> 82
Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
1 5 10 15
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Ser Ile Phe Ser Ile Tyr
20 25 30
Ala Met Gly Trp Tyr Arg Gln Ala Pro Gly Lys Gln Arg Glu Leu Val
35 40 45
Ala Ser Ile Ser Ser Gly Gly Gly Thr Asn Tyr Ala Asp Ser Val Lys
50 55 60
Gly Arg Phe Thr Ile Ser Gly Asp Asn Ala Lys Asn Thr Val Tyr Leu
65 70 75 80
Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys Lys
85 90 95
Arg Glu Asp Tyr Ser Ala Tyr Ala Pro Pro Ser Gly Ser Arg Gly Arg
100 105 110
Gly Thr Gln Val Thr Val Ser Ser His His His His His His
115 120 125
<210> 83
<211> 414
<212> DNA
<213> 未知(Unknown)
<220>
<223> 未知物种(Unknown species)
<220>
<221> CDS
<222> (1)..(414)
<400> 83
gca gaa gtt caa ctg gtt gaa tca ggc gga ggc ctg gtc caa gca ggc 48
Ala Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Ala Gly
1 5 10 15
gga tca ctg aga ctg tca tgc gca aca tca gga ccg aca ttt tca aca 96
Gly Ser Leu Arg Leu Ser Cys Ala Thr Ser Gly Pro Thr Phe Ser Thr
20 25 30
tat gca atg ggc tgg ttt aga caa gca ccg gga aaa gaa aga gaa ctg 144
Tyr Ala Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Leu
35 40 45
gtt gca gca att tca aga agc ggc atc ttt aca tat tat gcg gat tca 192
Val Ala Ala Ile Ser Arg Ser Gly Ile Phe Thr Tyr Tyr Ala Asp Ser
50 55 60
gtc aaa gga cgc ttt aca att agc aga gat aac gcg aaa aat aca gtc 240
Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Val
65 70 75 80
agc ctg caa atg aat tca ctg aaa ccg gaa gat aca gcg gtc tat tat 288
Ser Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr
85 90 95
tgc aat gca aga aga ttt ccg aga ggc gat tat tgg gga caa ggc aca 336
Cys Asn Ala Arg Arg Phe Pro Arg Gly Asp Tyr Trp Gly Gln Gly Thr
100 105 110
caa gtt aca gtt tca tca gaa ccg aaa aca ccg aaa ccg caa agc gga 384
Gln Val Thr Val Ser Ser Glu Pro Lys Thr Pro Lys Pro Gln Ser Gly
115 120 125
ggc gga agc cat cac cat cat cat cat taa 414
Gly Gly Ser His His His His His His
130 135
<210> 84
<211> 137
<212> PRT
<213> 未知(Unknown)
<220>
<223> 未知物种(Unknown species)
<400> 84
Ala Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Ala Gly
1 5 10 15
Gly Ser Leu Arg Leu Ser Cys Ala Thr Ser Gly Pro Thr Phe Ser Thr
20 25 30
Tyr Ala Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Leu
35 40 45
Val Ala Ala Ile Ser Arg Ser Gly Ile Phe Thr Tyr Tyr Ala Asp Ser
50 55 60
Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Val
65 70 75 80
Ser Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr
85 90 95
Cys Asn Ala Arg Arg Phe Pro Arg Gly Asp Tyr Trp Gly Gln Gly Thr
100 105 110
Gln Val Thr Val Ser Ser Glu Pro Lys Thr Pro Lys Pro Gln Ser Gly
115 120 125
Gly Gly Ser His His His His His His
130 135
<210> 85
<211> 438
<212> DNA
<213> 未知(Unknown)
<220>
<223> 未知物种(Unknown species)
<220>
<221> CDS
<222> (1)..(438)
<400> 85
gca gaa gtt caa ctg gtt gaa tca ggc gga gtt tca gtt aca gca ggc 48
Ala Glu Val Gln Leu Val Glu Ser Gly Gly Val Ser Val Thr Ala Gly
1 5 10 15
gga tca ctg aga ctg tca tgc gtt gca tca ggc aga aca tca tca att 96
Gly Ser Leu Arg Leu Ser Cys Val Ala Ser Gly Arg Thr Ser Ser Ile
20 25 30
tat gca atg ggc tgg ttt aga aga tca ccg gga aaa gaa aga gaa ttt 144
Tyr Ala Met Gly Trp Phe Arg Arg Ser Pro Gly Lys Glu Arg Glu Phe
35 40 45
gtt gca gca att ggc tgg aca gca ggc tca gca aca aca gca gat tca 192
Val Ala Ala Ile Gly Trp Thr Ala Gly Ser Ala Thr Thr Ala Asp Ser
50 55 60
gtt aaa ggc cgt ttt aca att agc cgt gat aac gcg aaa aac aca gtc 240
Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Val
65 70 75 80
tat ctg caa atg gat tca ctg aca ccg gaa gat aca gca gtt tat tac 288
Tyr Leu Gln Met Asp Ser Leu Thr Pro Glu Asp Thr Ala Val Tyr Tyr
85 90 95
tgc gca gca gca gat gtt ccg ctg gtt ccg atg ctg tgc gtt ccg tca 336
Cys Ala Ala Ala Asp Val Pro Leu Val Pro Met Leu Cys Val Pro Ser
100 105 110
gca tat gat gca tgg gga caa ggc aca caa gtt aca gtt tca tca gca 384
Ala Tyr Asp Ala Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser Ala
115 120 125
cat cat tca gag gat ccg aca ggc gga ggc tca cat cat cac cat cat 432
His His Ser Glu Asp Pro Thr Gly Gly Gly Ser His His His His His
130 135 140
cat taa 438
His
145
<210> 86
<211> 145
<212> PRT
<213> 未知(Unknown)
<220>
<223> 未知物种(Unknown species)
<400> 86
Ala Glu Val Gln Leu Val Glu Ser Gly Gly Val Ser Val Thr Ala Gly
1 5 10 15
Gly Ser Leu Arg Leu Ser Cys Val Ala Ser Gly Arg Thr Ser Ser Ile
20 25 30
Tyr Ala Met Gly Trp Phe Arg Arg Ser Pro Gly Lys Glu Arg Glu Phe
35 40 45
Val Ala Ala Ile Gly Trp Thr Ala Gly Ser Ala Thr Thr Ala Asp Ser
50 55 60
Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Val
65 70 75 80
Tyr Leu Gln Met Asp Ser Leu Thr Pro Glu Asp Thr Ala Val Tyr Tyr
85 90 95
Cys Ala Ala Ala Asp Val Pro Leu Val Pro Met Leu Cys Val Pro Ser
100 105 110
Ala Tyr Asp Ala Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser Ala
115 120 125
His His Ser Glu Asp Pro Thr Gly Gly Gly Ser His His His His His
130 135 140
His
145
<210> 87
<211> 396
<212> DNA
<213> 未知(Unknown)
<220>
<223> 未知物种(Unknown species)
<220>
<221> CDS
<222> (1)..(396)
<400> 87
gca gaa gtt caa ctg gtt gaa tca ggc gga ggc ctg gtc caa aca ggc 48
Ala Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Thr Gly
1 5 10 15
gga tca ctg aga ctg tca tgc aca gca tca ggc tgc ttt tat agc att 96
Gly Ser Leu Arg Leu Ser Cys Thr Ala Ser Gly Cys Phe Tyr Ser Ile
20 25 30
gat att atg ggc tgg tat aga cag aca ccg gga aaa caa aga gaa ccg 144
Asp Ile Met Gly Trp Tyr Arg Gln Thr Pro Gly Lys Gln Arg Glu Pro
35 40 45
gtt gca ctg att aca tca gat ggc aca aca aat tat gcc atg tca gtc 192
Val Ala Leu Ile Thr Ser Asp Gly Thr Thr Asn Tyr Ala Met Ser Val
50 55 60
aaa gga cgc ttt aca att tca cgc gat aat gcg aaa aac acg gtc tat 240
Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Val Tyr
65 70 75 80
ctg caa atg gat aat ctg aaa ccg gaa gat aca gcg gtc tat tat tgg 288
Leu Gln Met Asp Asn Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Trp
85 90 95
gca gtt ggc aga tat ttt ggc caa ggc aca caa gtt aca gtt tca tca 336
Ala Val Gly Arg Tyr Phe Gly Gln Gly Thr Gln Val Thr Val Ser Ser
100 105 110
gaa ccg aaa aca ccg aaa ccg caa agc gga ggc gga agc cat cac cat 384
Glu Pro Lys Thr Pro Lys Pro Gln Ser Gly Gly Gly Ser His His His
115 120 125
cat cat cat taa 396
His His His
130
<210> 88
<211> 131
<212> PRT
<213> 未知(Unknown)
<220>
<223> 未知物种(Unknown species)
<400> 88
Ala Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Thr Gly
1 5 10 15
Gly Ser Leu Arg Leu Ser Cys Thr Ala Ser Gly Cys Phe Tyr Ser Ile
20 25 30
Asp Ile Met Gly Trp Tyr Arg Gln Thr Pro Gly Lys Gln Arg Glu Pro
35 40 45
Val Ala Leu Ile Thr Ser Asp Gly Thr Thr Asn Tyr Ala Met Ser Val
50 55 60
Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Val Tyr
65 70 75 80
Leu Gln Met Asp Asn Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Trp
85 90 95
Ala Val Gly Arg Tyr Phe Gly Gln Gly Thr Gln Val Thr Val Ser Ser
100 105 110
Glu Pro Lys Thr Pro Lys Pro Gln Ser Gly Gly Gly Ser His His His
115 120 125
His His His
130
<210> 89
<211> 432
<212> DNA
<213> 未知(Unknown)
<220>
<223> 未知物种(Unknown species)
<220>
<221> CDS
<222> (1)..(432)
<400> 89
gca gaa gtt caa ctg gtt gaa tca ggc gga ggc ctg gtt caa gca ggc 48
Ala Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Ala Gly
1 5 10 15
gat tca ctg aga ctg tca tgc aca gca tca ggc aga aca ttt aga gat 96
Asp Ser Leu Arg Leu Ser Cys Thr Ala Ser Gly Arg Thr Phe Arg Asp
20 25 30
tat gca gtt gca tgg gtt aga caa gca ccg gga aaa gca aga gaa ttt 144
Tyr Ala Val Ala Trp Val Arg Gln Ala Pro Gly Lys Ala Arg Glu Phe
35 40 45
gtt gca gca att aca tgg gga ggc gat ttc aca aga tat gca gat tca 192
Val Ala Ala Ile Thr Trp Gly Gly Asp Phe Thr Arg Tyr Ala Asp Ser
50 55 60
gtt aaa gga cgc ttt acg atc ttt cgc gat aat gcg aaa aat acg gtt 240
Val Lys Gly Arg Phe Thr Ile Phe Arg Asp Asn Ala Lys Asn Thr Val
65 70 75 80
tac ctg ctg atg aat tca ctg aaa ccg gaa gat aca gcg aaa tat tac 288
Tyr Leu Leu Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Lys Tyr Tyr
85 90 95
tgc gca gca gat tat tca tgc ggc tca gat tgg aat aca tgg atg ccg 336
Cys Ala Ala Asp Tyr Ser Cys Gly Ser Asp Trp Asn Thr Trp Met Pro
100 105 110
tat tgg ggc aga ggc aca caa gtt aca gtt tca tca gaa ccg aaa aca 384
Tyr Trp Gly Arg Gly Thr Gln Val Thr Val Ser Ser Glu Pro Lys Thr
115 120 125
ccg aaa ccg caa agc gga ggc gga tca cat cat cac cat cat cat taa 432
Pro Lys Pro Gln Ser Gly Gly Gly Ser His His His His His His
130 135 140
<210> 90
<211> 143
<212> PRT
<213> 未知(Unknown)
<220>
<223> 未知物种(Unknown species)
<400> 90
Ala Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Ala Gly
1 5 10 15
Asp Ser Leu Arg Leu Ser Cys Thr Ala Ser Gly Arg Thr Phe Arg Asp
20 25 30
Tyr Ala Val Ala Trp Val Arg Gln Ala Pro Gly Lys Ala Arg Glu Phe
35 40 45
Val Ala Ala Ile Thr Trp Gly Gly Asp Phe Thr Arg Tyr Ala Asp Ser
50 55 60
Val Lys Gly Arg Phe Thr Ile Phe Arg Asp Asn Ala Lys Asn Thr Val
65 70 75 80
Tyr Leu Leu Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Lys Tyr Tyr
85 90 95
Cys Ala Ala Asp Tyr Ser Cys Gly Ser Asp Trp Asn Thr Trp Met Pro
100 105 110
Tyr Trp Gly Arg Gly Thr Gln Val Thr Val Ser Ser Glu Pro Lys Thr
115 120 125
Pro Lys Pro Gln Ser Gly Gly Gly Ser His His His His His His
130 135 140
<210> 91
<211> 405
<212> DNA
<213> 未知(Unknown)
<220>
<223> 未知物种(Unknown species)
<220>
<221> CDS
<222> (1)..(405)
<400> 91
gca gaa gtt caa ctg gtt gaa tca ggc gga ggc tgg gtt caa gca ggc 48
Ala Glu Val Gln Leu Val Glu Ser Gly Gly Gly Trp Val Gln Ala Gly
1 5 10 15
gga tca ctg aga ctg tca tgc aca ggc tca ggc aga aca ttt tca tca 96
Gly Ser Leu Arg Leu Ser Cys Thr Gly Ser Gly Arg Thr Phe Ser Ser
20 25 30
tat gca atg ggc tgg ttt aga caa gca ccg gga aaa gca aga gaa ttt 144
Tyr Ala Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Ala Arg Glu Phe
35 40 45
gtt gca gca ctg aga aga tca ggc aca tat tat gca gat tca gtc aaa 192
Val Ala Ala Leu Arg Arg Ser Gly Thr Tyr Tyr Ala Asp Ser Val Lys
50 55 60
gga cgc ttc acg atc ttc aaa gat aat gcg aaa aac acg gtc ttt ctg 240
Gly Arg Phe Thr Ile Phe Lys Asp Asn Ala Lys Asn Thr Val Phe Leu
65 70 75 80
caa atg tca tca ctg tct ccg gaa gat aca gca gtc tat tat tgc aat 288
Gln Met Ser Ser Leu Ser Pro Glu Asp Thr Ala Val Tyr Tyr Cys Asn
85 90 95
ctg tgg gtt gga ccg tat gaa tat tgg gga caa ggc aca caa gtt aca 336
Leu Trp Val Gly Pro Tyr Glu Tyr Trp Gly Gln Gly Thr Gln Val Thr
100 105 110
gtt tca tca gaa ccg aaa aca ccg aaa ccg caa agc gga ggc gga agc 384
Val Ser Ser Glu Pro Lys Thr Pro Lys Pro Gln Ser Gly Gly Gly Ser
115 120 125
cat cac cat cat cat cat taa 405
His His His His His His
130
<210> 92
<211> 134
<212> PRT
<213> 未知(Unknown)
<220>
<223> 未知物种(Unknown species)
<400> 92
Ala Glu Val Gln Leu Val Glu Ser Gly Gly Gly Trp Val Gln Ala Gly
1 5 10 15
Gly Ser Leu Arg Leu Ser Cys Thr Gly Ser Gly Arg Thr Phe Ser Ser
20 25 30
Tyr Ala Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Ala Arg Glu Phe
35 40 45
Val Ala Ala Leu Arg Arg Ser Gly Thr Tyr Tyr Ala Asp Ser Val Lys
50 55 60
Gly Arg Phe Thr Ile Phe Lys Asp Asn Ala Lys Asn Thr Val Phe Leu
65 70 75 80
Gln Met Ser Ser Leu Ser Pro Glu Asp Thr Ala Val Tyr Tyr Cys Asn
85 90 95
Leu Trp Val Gly Pro Tyr Glu Tyr Trp Gly Gln Gly Thr Gln Val Thr
100 105 110
Val Ser Ser Glu Pro Lys Thr Pro Lys Pro Gln Ser Gly Gly Gly Ser
115 120 125
His His His His His His
130
<210> 93
<211> 444
<212> DNA
<213> 未知(Unknown)
<220>
<223> 未知物种(Unknown species)
<220>
<221> CDS
<222> (1)..(444)
<400> 93
gca gaa gtt caa ctg gtt gaa tca ggc gga ggc ctg gtc caa gca ggc 48
Ala Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Ala Gly
1 5 10 15
gga tca ctg aga ctg tca tgc gca gtt tca ggc gca aca ctg gat gat 96
Gly Ser Leu Arg Leu Ser Cys Ala Val Ser Gly Ala Thr Leu Asp Asp
20 25 30
att gat att ggc tgg ttt aga cgc gca ccg gga aaa gaa aga gaa ggc 144
Ile Asp Ile Gly Trp Phe Arg Arg Ala Pro Gly Lys Glu Arg Glu Gly
35 40 45
gtt agc tgc att gca aga aga ggc aca caa acg ttt tat gat gat agc 192
Val Ser Cys Ile Ala Arg Arg Gly Thr Gln Thr Phe Tyr Asp Asp Ser
50 55 60
gtc aaa gga cgc ttt acg att ttt cgc gat aac gca aaa aac acg gtc 240
Val Lys Gly Arg Phe Thr Ile Phe Arg Asp Asn Ala Lys Asn Thr Val
65 70 75 80
tac ctg caa atg aat aat ctg aaa ccg gaa gat aca gcg gtc tat tat 288
Tyr Leu Gln Met Asn Asn Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr
85 90 95
tgc gca gca gca aaa aat aga ctg ttt aga caa tgg gtt ctg agc caa 336
Cys Ala Ala Ala Lys Asn Arg Leu Phe Arg Gln Trp Val Leu Ser Gln
100 105 110
ctg tgg ttt gat tat tgg gga caa ggc aca cag gtt aca gtt tca tca 384
Leu Trp Phe Asp Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser
115 120 125
gaa ccg aaa aca ccg aaa ccg caa agc gga ggc gga agc cat cac cat 432
Glu Pro Lys Thr Pro Lys Pro Gln Ser Gly Gly Gly Ser His His His
130 135 140
cat cat cat taa 444
His His His
145
<210> 94
<211> 147
<212> PRT
<213> 未知(Unknown)
<220>
<223> 未知物种(Unknown species)
<400> 94
Ala Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Ala Gly
1 5 10 15
Gly Ser Leu Arg Leu Ser Cys Ala Val Ser Gly Ala Thr Leu Asp Asp
20 25 30
Ile Asp Ile Gly Trp Phe Arg Arg Ala Pro Gly Lys Glu Arg Glu Gly
35 40 45
Val Ser Cys Ile Ala Arg Arg Gly Thr Gln Thr Phe Tyr Asp Asp Ser
50 55 60
Val Lys Gly Arg Phe Thr Ile Phe Arg Asp Asn Ala Lys Asn Thr Val
65 70 75 80
Tyr Leu Gln Met Asn Asn Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr
85 90 95
Cys Ala Ala Ala Lys Asn Arg Leu Phe Arg Gln Trp Val Leu Ser Gln
100 105 110
Leu Trp Phe Asp Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser
115 120 125
Glu Pro Lys Thr Pro Lys Pro Gln Ser Gly Gly Gly Ser His His His
130 135 140
His His His
145
<210> 95
<211> 423
<212> DNA
<213> 未知(Unknown)
<220>
<223> 未知物种(Unknown species)
<220>
<221> CDS
<222> (1)..(423)
<400> 95
gca gaa gtt caa ctg gtt gaa tca ggc gga ggc ctg gtc caa gca ggc 48
Ala Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Ala Gly
1 5 10 15
gga tca ctg aga ctg tca tgc gca gca tca ggc aga aca ttt tca tca 96
Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Thr Phe Ser Ser
20 25 30
tat gca atg ggc tgg ttt aga caa gca ccg gga aaa gca aga gaa ttt 144
Tyr Ala Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Ala Arg Glu Phe
35 40 45
gtt gca gca ctg aga aga tca ggc aca tat tat gca gat tca gtc aaa 192
Val Ala Ala Leu Arg Arg Ser Gly Thr Tyr Tyr Ala Asp Ser Val Lys
50 55 60
gga cgc ttt aca att agc aga gat aat gca cgc aat acg gtc tat ctg 240
Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Arg Asn Thr Val Tyr Leu
65 70 75 80
caa atg cat aat ctg atc ccg gaa gat aca ggc gtc tat tat tgc gca 288
Gln Met His Asn Leu Ile Pro Glu Asp Thr Gly Val Tyr Tyr Cys Ala
85 90 95
ggc gaa att ctg ggc aga tca tgc aga gaa gat gat tat gat tat tgg 336
Gly Glu Ile Leu Gly Arg Ser Cys Arg Glu Asp Asp Tyr Asp Tyr Trp
100 105 110
gga caa ggc aca caa gtt aca gtt tca tca gca cat cat tca gag gat 384
Gly Gln Gly Thr Gln Val Thr Val Ser Ser Ala His His Ser Glu Asp
115 120 125
ccg aca ggc ggt ggc tca cat cat cac cat cat cat taa 423
Pro Thr Gly Gly Gly Ser His His His His His His
130 135 140
<210> 96
<211> 140
<212> PRT
<213> 未知(Unknown)
<220>
<223> 未知物种(Unknown species)
<400> 96
Ala Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Ala Gly
1 5 10 15
Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Thr Phe Ser Ser
20 25 30
Tyr Ala Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Ala Arg Glu Phe
35 40 45
Val Ala Ala Leu Arg Arg Ser Gly Thr Tyr Tyr Ala Asp Ser Val Lys
50 55 60
Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Arg Asn Thr Val Tyr Leu
65 70 75 80
Gln Met His Asn Leu Ile Pro Glu Asp Thr Gly Val Tyr Tyr Cys Ala
85 90 95
Gly Glu Ile Leu Gly Arg Ser Cys Arg Glu Asp Asp Tyr Asp Tyr Trp
100 105 110
Gly Gln Gly Thr Gln Val Thr Val Ser Ser Ala His His Ser Glu Asp
115 120 125
Pro Thr Gly Gly Gly Ser His His His His His His
130 135 140
<210> 97
<211> 426
<212> DNA
<213> 未知(Unknown)
<220>
<223> 未知物种(Unknown species)
<220>
<221> CDS
<222> (1)..(426)
<400> 97
gca gaa gtt caa ctg gtt gaa tca ggc gga ggc ctg gtt caa aca ggc 48
Ala Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Thr Gly
1 5 10 15
gca tca ctg aga att tca tgc gca gtt aca ggc caa aca ctg aaa aaa 96
Ala Ser Leu Arg Ile Ser Cys Ala Val Thr Gly Gln Thr Leu Lys Lys
20 25 30
tac aca gtt ggc tgg cat aga caa gca ccg gga aaa gaa aga gaa ttt 144
Tyr Thr Val Gly Trp His Arg Gln Ala Pro Gly Lys Glu Arg Glu Phe
35 40 45
gtt gca gtt att ggc tgg aca tca ggc gat aca cat tat gca gca tca 192
Val Ala Val Ile Gly Trp Thr Ser Gly Asp Thr His Tyr Ala Ala Ser
50 55 60
gtt aaa gga cgc ttt acg atc tca aaa gac aac gcg aaa aat acg gtc 240
Val Lys Gly Arg Phe Thr Ile Ser Lys Asp Asn Ala Lys Asn Thr Val
65 70 75 80
tac ctg caa atg aat tca ctg aaa ccg gaa gat aca gcg gtc tat tat 288
Tyr Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr
85 90 95
tgc gtt gca gat ccg tat aca tca atg tgc aga cat gtt ccg tat tgg 336
Cys Val Ala Asp Pro Tyr Thr Ser Met Cys Arg His Val Pro Tyr Trp
100 105 110
gga caa ggc att caa gtt aca gtt tca tca gaa ccg aaa aca ccg aaa 384
Gly Gln Gly Ile Gln Val Thr Val Ser Ser Glu Pro Lys Thr Pro Lys
115 120 125
ccg caa agc gga ggc gga tca cat cat cac cat cat cat taa 426
Pro Gln Ser Gly Gly Gly Ser His His His His His His
130 135 140
<210> 98
<211> 141
<212> PRT
<213> 未知(Unknown)
<220>
<223> 未知物种(Unknown species)
<400> 98
Ala Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Thr Gly
1 5 10 15
Ala Ser Leu Arg Ile Ser Cys Ala Val Thr Gly Gln Thr Leu Lys Lys
20 25 30
Tyr Thr Val Gly Trp His Arg Gln Ala Pro Gly Lys Glu Arg Glu Phe
35 40 45
Val Ala Val Ile Gly Trp Thr Ser Gly Asp Thr His Tyr Ala Ala Ser
50 55 60
Val Lys Gly Arg Phe Thr Ile Ser Lys Asp Asn Ala Lys Asn Thr Val
65 70 75 80
Tyr Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr
85 90 95
Cys Val Ala Asp Pro Tyr Thr Ser Met Cys Arg His Val Pro Tyr Trp
100 105 110
Gly Gln Gly Ile Gln Val Thr Val Ser Ser Glu Pro Lys Thr Pro Lys
115 120 125
Pro Gln Ser Gly Gly Gly Ser His His His His His His
130 135 140
<210> 99
<211> 420
<212> DNA
<213> 未知(Unknown)
<220>
<223> 未知物种(Unknown species)
<220>
<221> CDS
<222> (1)..(420)
<400> 99
gca gaa gtt caa ctg gtt gaa tca ggc gga ggc gaa gtc caa cct ggc 48
Ala Glu Val Gln Leu Val Glu Ser Gly Gly Gly Glu Val Gln Pro Gly
1 5 10 15
gga tca ctg aaa ctg tca tgc gtt gca tca ggc gat att ttt cgc att 96
Gly Ser Leu Lys Leu Ser Cys Val Ala Ser Gly Asp Ile Phe Arg Ile
20 25 30
aat gtc att gcg tgg tat aga caa gca ctg ggc aaa caa aga gaa ctg 144
Asn Val Ile Ala Trp Tyr Arg Gln Ala Leu Gly Lys Gln Arg Glu Leu
35 40 45
gtt gct tgg gca gca gcg gaa ggc gat aca gat tat ggc aga tca gtt 192
Val Ala Trp Ala Ala Ala Glu Gly Asp Thr Asp Tyr Gly Arg Ser Val
50 55 60
aaa ggc cgt ttt agc att tca cgc gat aat ccg aaa aac aca gca tac 240
Lys Gly Arg Phe Ser Ile Ser Arg Asp Asn Pro Lys Asn Thr Ala Tyr
65 70 75 80
ctg caa atg aat tca ctg aag ccg gaa gat aca gca gtc tat tat tgc 288
Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys
85 90 95
gcg ttt cag aat tat ggc tca tca tgg tat gaa ggc gat tat tgg ggc 336
Ala Phe Gln Asn Tyr Gly Ser Ser Trp Tyr Glu Gly Asp Tyr Trp Gly
100 105 110
aga ggc aca caa gtt aca gtt tca tca gca cat cat tca gag gat ccg 384
Arg Gly Thr Gln Val Thr Val Ser Ser Ala His His Ser Glu Asp Pro
115 120 125
aca ggc ggt ggc tca cat cat cac cat cat cat taa 420
Thr Gly Gly Gly Ser His His His His His His
130 135
<210> 100
<211> 139
<212> PRT
<213> 未知(Unknown)
<220>
<223> 未知物种(Unknown species)
<400> 100
Ala Glu Val Gln Leu Val Glu Ser Gly Gly Gly Glu Val Gln Pro Gly
1 5 10 15
Gly Ser Leu Lys Leu Ser Cys Val Ala Ser Gly Asp Ile Phe Arg Ile
20 25 30
Asn Val Ile Ala Trp Tyr Arg Gln Ala Leu Gly Lys Gln Arg Glu Leu
35 40 45
Val Ala Trp Ala Ala Ala Glu Gly Asp Thr Asp Tyr Gly Arg Ser Val
50 55 60
Lys Gly Arg Phe Ser Ile Ser Arg Asp Asn Pro Lys Asn Thr Ala Tyr
65 70 75 80
Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys
85 90 95
Ala Phe Gln Asn Tyr Gly Ser Ser Trp Tyr Glu Gly Asp Tyr Trp Gly
100 105 110
Arg Gly Thr Gln Val Thr Val Ser Ser Ala His His Ser Glu Asp Pro
115 120 125
Thr Gly Gly Gly Ser His His His His His His
130 135
<210> 101
<211> 399
<212> DNA
<213> 未知(Unknown)
<220>
<223> 未知物种(Unknown species)
<220>
<221> CDS
<222> (1)..(399)
<400> 101
gca gaa gtt caa ctg gtt gaa tca ggc gga ggc ctg gtt caa cct ggc 48
Ala Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly
1 5 10 15
gga tca ctg aga ctg tca tgc gca gca tca ggc ttt aca ctg gat aca 96
Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Leu Asp Thr
20 25 30
tac aaa att ggc tgg ttt cac gaa aca ccg gaa aaa ggc aga gaa ggc 144
Tyr Lys Ile Gly Trp Phe His Glu Thr Pro Glu Lys Gly Arg Glu Gly
35 40 45
gtt tca tgc att tca gtt tca aca ggc aca gca tca tat gtc gat tca 192
Val Ser Cys Ile Ser Val Ser Thr Gly Thr Ala Ser Tyr Val Asp Ser
50 55 60
gtt aaa gga cgc ttt acg att tca atc gat agc gcg aaa aat acg gtc 240
Val Lys Gly Arg Phe Thr Ile Ser Ile Asp Ser Ala Lys Asn Thr Val
65 70 75 80
tac ctg caa atg aat aat ctg aaa ccg gaa gat agc gca gtc tat aga 288
Tyr Leu Gln Met Asn Asn Leu Lys Pro Glu Asp Ser Ala Val Tyr Arg
85 90 95
tgc atg gca aga ggc gtt tgg gga ctg ggc aca caa gtt aca gtt tca 336
Cys Met Ala Arg Gly Val Trp Gly Leu Gly Thr Gln Val Thr Val Ser
100 105 110
tca gaa ccg aaa aca ccg aaa ccg caa agc gga ggc gga agc cat cac 384
Ser Glu Pro Lys Thr Pro Lys Pro Gln Ser Gly Gly Gly Ser His His
115 120 125
cat cat cat cat taa 399
His His His His
130
<210> 102
<211> 132
<212> PRT
<213> 未知(Unknown)
<220>
<223> 未知物种(Unknown species)
<400> 102
Ala Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly
1 5 10 15
Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Leu Asp Thr
20 25 30
Tyr Lys Ile Gly Trp Phe His Glu Thr Pro Glu Lys Gly Arg Glu Gly
35 40 45
Val Ser Cys Ile Ser Val Ser Thr Gly Thr Ala Ser Tyr Val Asp Ser
50 55 60
Val Lys Gly Arg Phe Thr Ile Ser Ile Asp Ser Ala Lys Asn Thr Val
65 70 75 80
Tyr Leu Gln Met Asn Asn Leu Lys Pro Glu Asp Ser Ala Val Tyr Arg
85 90 95
Cys Met Ala Arg Gly Val Trp Gly Leu Gly Thr Gln Val Thr Val Ser
100 105 110
Ser Glu Pro Lys Thr Pro Lys Pro Gln Ser Gly Gly Gly Ser His His
115 120 125
His His His His
130
<210> 103
<211> 414
<212> DNA
<213> 未知(Unknown)
<220>
<223> 未知物种(Unknown species)
<220>
<221> CDS
<222> (1)..(414)
<400> 103
gca gaa gtt caa ctg gtt gaa tca ggc gga ggc ctg gtc caa aca ggc 48
Ala Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Thr Gly
1 5 10 15
gga tca ctg aga ctg tca tgc gaa gtt aca ggc ttt ccg gtt gat tca 96
Gly Ser Leu Arg Leu Ser Cys Glu Val Thr Gly Phe Pro Val Asp Ser
20 25 30
tat gac att ggc tgg ttt aga ctg gca ccg gga aaa gat aga gaa ggc 144
Tyr Asp Ile Gly Trp Phe Arg Leu Ala Pro Gly Lys Asp Arg Glu Gly
35 40 45
gtt gca tgc att tca aat aga aat ggc aga agc atg tat agc gat agc 192
Val Ala Cys Ile Ser Asn Arg Asn Gly Arg Ser Met Tyr Ser Asp Ser
50 55 60
gtt aaa ggc cgt ttt aca att agc cgt gat atc gcg aaa aac aca gtc 240
Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Ile Ala Lys Asn Thr Val
65 70 75 80
tat ctg caa atg aat agc ctg aaa agc gaa gat acg gcg atc tat tat 288
Tyr Leu Gln Met Asn Ser Leu Lys Ser Glu Asp Thr Ala Ile Tyr Tyr
85 90 95
tgc gca ctt gca ccg caa tat ggc ttt ctg gca tat tgg gga caa ggc 336
Cys Ala Leu Ala Pro Gln Tyr Gly Phe Leu Ala Tyr Trp Gly Gln Gly
100 105 110
aca caa gtt aca gtt tca tca gca cat cat tca gag gat ccg aca ggc 384
Thr Gln Val Thr Val Ser Ser Ala His His Ser Glu Asp Pro Thr Gly
115 120 125
ggt ggc tca cat cat cac cat cat cat taa 414
Gly Gly Ser His His His His His His
130 135
<210> 104
<211> 137
<212> PRT
<213> 未知(Unknown)
<220>
<223> 未知物种(Unknown species)
<400> 104
Ala Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Thr Gly
1 5 10 15
Gly Ser Leu Arg Leu Ser Cys Glu Val Thr Gly Phe Pro Val Asp Ser
20 25 30
Tyr Asp Ile Gly Trp Phe Arg Leu Ala Pro Gly Lys Asp Arg Glu Gly
35 40 45
Val Ala Cys Ile Ser Asn Arg Asn Gly Arg Ser Met Tyr Ser Asp Ser
50 55 60
Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Ile Ala Lys Asn Thr Val
65 70 75 80
Tyr Leu Gln Met Asn Ser Leu Lys Ser Glu Asp Thr Ala Ile Tyr Tyr
85 90 95
Cys Ala Leu Ala Pro Gln Tyr Gly Phe Leu Ala Tyr Trp Gly Gln Gly
100 105 110
Thr Gln Val Thr Val Ser Ser Ala His His Ser Glu Asp Pro Thr Gly
115 120 125
Gly Gly Ser His His His His His His
130 135
<210> 105
<211> 417
<212> DNA
<213> 未知(Unknown)
<220>
<223> 未知物种(Unknown species)
<220>
<221> CDS
<222> (1)..(417)
<400> 105
gca gaa gtt caa ctg gtt gaa tca ggc gga ggc tca gtt caa aca ggc 48
Ala Glu Val Gln Leu Val Glu Ser Gly Gly Gly Ser Val Gln Thr Gly
1 5 10 15
gga tca ctg aga ctg tca tgc gca gca tca ggc aga aca ttt tca tca 96
Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Thr Phe Ser Ser
20 25 30
tat gtt atg ggc tgg ttt aga caa gca ccg gga aaa gaa aga gaa ttt 144
Tyr Val Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Phe
35 40 45
gtt gca gca att tca tgg tca ggc gga agc aca ctg tat gca gat tca 192
Val Ala Ala Ile Ser Trp Ser Gly Gly Ser Thr Leu Tyr Ala Asp Ser
50 55 60
gtt aaa gga cgc ttt aca atc agc aga gat aac gcg aaa aat acg gtc 240
Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Val
65 70 75 80
tat ctg caa atg aat tca ctg aaa ccg gaa gat aca gcg gtc tat tat 288
Tyr Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr
85 90 95
tgc tat gca cat aga ctg tgg cct ctg aca gat tat tgg gga caa ggc 336
Cys Tyr Ala His Arg Leu Trp Pro Leu Thr Asp Tyr Trp Gly Gln Gly
100 105 110
aca caa gtt aca gtt tca tca gaa ccg aaa aca ccg aaa ccg caa tct 384
Thr Gln Val Thr Val Ser Ser Glu Pro Lys Thr Pro Lys Pro Gln Ser
115 120 125
ggc gga gga tca cat cat cac cat cat cat taa 417
Gly Gly Gly Ser His His His His His His
130 135
<210> 106
<211> 138
<212> PRT
<213> 未知(Unknown)
<220>
<223> 未知物种(Unknown species)
<400> 106
Ala Glu Val Gln Leu Val Glu Ser Gly Gly Gly Ser Val Gln Thr Gly
1 5 10 15
Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Thr Phe Ser Ser
20 25 30
Tyr Val Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Phe
35 40 45
Val Ala Ala Ile Ser Trp Ser Gly Gly Ser Thr Leu Tyr Ala Asp Ser
50 55 60
Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Val
65 70 75 80
Tyr Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr
85 90 95
Cys Tyr Ala His Arg Leu Trp Pro Leu Thr Asp Tyr Trp Gly Gln Gly
100 105 110
Thr Gln Val Thr Val Ser Ser Glu Pro Lys Thr Pro Lys Pro Gln Ser
115 120 125
Gly Gly Gly Ser His His His His His His
130 135
<210> 107
<211> 426
<212> DNA
<213> 未知(Unknown)
<220>
<223> 未知物种(Unknown species)
<220>
<221> CDS
<222> (1)..(426)
<400> 107
gca gaa gtt caa ctg gtt gaa tca ggc gga ggc ctg gtt caa agc gga 48
Ala Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Ser Gly
1 5 10 15
ggc tca ctg aga ctg tca tgc gca gca tca ggc ttt aat ctg gat tat 96
Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Asn Leu Asp Tyr
20 25 30
tat gca gtt ggc tgg ttt aga caa gca ccg gga aaa gaa aga gaa ggc 144
Tyr Ala Val Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Gly
35 40 45
gtt agc tgc att aac aaa gat gat gat aca acg aaa agc gca gat agc 192
Val Ser Cys Ile Asn Lys Asp Asp Asp Thr Thr Lys Ser Ala Asp Ser
50 55 60
gtt aaa gga cgc ttt aca att agc aga gat aac gcg aaa aac acg gtc 240
Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Val
65 70 75 80
tat ctg caa atg aat tca ctg aaa ccg gaa gat aca gcg gtc tat tat 288
Tyr Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr
85 90 95
tgc aat gca ctg aca tat tca ccg aca ggc tgg gga ccg ctt ggc tca 336
Cys Asn Ala Leu Thr Tyr Ser Pro Thr Gly Trp Gly Pro Leu Gly Ser
100 105 110
tgg gga caa ggc aca caa gtt aca gtt tca tca gca cat cat tca gag 384
Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser Ala His His Ser Glu
115 120 125
gat ccg aca ggc ggt ggc tca cat cat cac cat cat cat taa 426
Asp Pro Thr Gly Gly Gly Ser His His His His His His
130 135 140
<210> 108
<211> 141
<212> PRT
<213> 未知(Unknown)
<220>
<223> 未知物种(Unknown species)
<400> 108
Ala Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Ser Gly
1 5 10 15
Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Asn Leu Asp Tyr
20 25 30
Tyr Ala Val Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Gly
35 40 45
Val Ser Cys Ile Asn Lys Asp Asp Asp Thr Thr Lys Ser Ala Asp Ser
50 55 60
Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Val
65 70 75 80
Tyr Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr
85 90 95
Cys Asn Ala Leu Thr Tyr Ser Pro Thr Gly Trp Gly Pro Leu Gly Ser
100 105 110
Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser Ala His His Ser Glu
115 120 125
Asp Pro Thr Gly Gly Gly Ser His His His His His His
130 135 140
<210> 109
<211> 399
<212> DNA
<213> 未知(Unknown)
<220>
<223> 未知物种(Unknown species)
<220>
<221> CDS
<222> (1)..(399)
<400> 109
gcg gaa gtt cag ctg gtt gag tca gga gga gga cag gtc gaa act ggt 48
Ala Glu Val Gln Leu Val Glu Ser Gly Gly Gly Gln Val Glu Thr Gly
1 5 10 15
gga agc ttg aga ctg tcc tgt caa gca agt ggc tca acc ttc agc gac 96
Gly Ser Leu Arg Leu Ser Cys Gln Ala Ser Gly Ser Thr Phe Ser Asp
20 25 30
tat gtg atg gct tgg ttt cgc caa aga cct ggc aaa gaa cgg gag ttt 144
Tyr Val Met Ala Trp Phe Arg Gln Arg Pro Gly Lys Glu Arg Glu Phe
35 40 45
gtt gcg aca att tcc cgt aat ggc ggt act aca acc tat ggg tcg tct 192
Val Ala Thr Ile Ser Arg Asn Gly Gly Thr Thr Thr Tyr Gly Ser Ser
50 55 60
gtg aaa ggg agg ttt acg atc tct cgc gat aat gcc atg aaa acg gtc 240
Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Met Lys Thr Val
65 70 75 80
tat ctc cag atg aac tct ctt aag ccg gaa gat aca ggc gta tac tac 288
Tyr Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Gly Val Tyr Tyr
85 90 95
tgc gat gca tta tgg cct cca atg cac tat cga ggt caa ggc aca caa 336
Cys Asp Ala Leu Trp Pro Pro Met His Tyr Arg Gly Gln Gly Thr Gln
100 105 110
gtg acg gta agc tca gaa ccc aag aca ccg aaa ccg cag agt cat cat 384
Val Thr Val Ser Ser Glu Pro Lys Thr Pro Lys Pro Gln Ser His His
115 120 125
cac cat cat cat taa 399
His His His His
130
<210> 110
<211> 132
<212> PRT
<213> 未知(Unknown)
<220>
<223> 未知物种(Unknown species)
<400> 110
Ala Glu Val Gln Leu Val Glu Ser Gly Gly Gly Gln Val Glu Thr Gly
1 5 10 15
Gly Ser Leu Arg Leu Ser Cys Gln Ala Ser Gly Ser Thr Phe Ser Asp
20 25 30
Tyr Val Met Ala Trp Phe Arg Gln Arg Pro Gly Lys Glu Arg Glu Phe
35 40 45
Val Ala Thr Ile Ser Arg Asn Gly Gly Thr Thr Thr Tyr Gly Ser Ser
50 55 60
Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Met Lys Thr Val
65 70 75 80
Tyr Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Gly Val Tyr Tyr
85 90 95
Cys Asp Ala Leu Trp Pro Pro Met His Tyr Arg Gly Gln Gly Thr Gln
100 105 110
Val Thr Val Ser Ser Glu Pro Lys Thr Pro Lys Pro Gln Ser His His
115 120 125
His His His His
130
<210> 111
<211> 420
<212> DNA
<213> 未知(Unknown)
<220>
<223> 未知物种(Unknown species)
<220>
<221> CDS
<222> (1)..(420)
<400> 111
gca gaa gtt caa ctg gtt gaa tca ggc gga ggc ctg gtt caa cct ggc 48
Ala Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly
1 5 10 15
gga tca ctg aga ctg tca tgc gca gca tca ggc ctt aca ttt tca tca 96
Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Leu Thr Phe Ser Ser
20 25 30
tat gca atg ggc tgg ttt aga caa gca ccg gga aaa gaa aga gaa ttt 144
Tyr Ala Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Phe
35 40 45
gtt gca gca att tca tgg tca ggc gat agc aca tat tat gca gat tca 192
Val Ala Ala Ile Ser Trp Ser Gly Asp Ser Thr Tyr Tyr Ala Asp Ser
50 55 60
gtc aaa gga cgc ttt aca att agc aga gat aac gcg aaa aat aca gtc 240
Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Val
65 70 75 80
agc ctg cag atg aat aat ctg aaa ccg gaa gat aca gcg gtc tat tat 288
Ser Leu Gln Met Asn Asn Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr
85 90 95
tgc gca aaa tgg gga gtt gca gga ccg tgg tat ctg gaa gtt tgg gga 336
Cys Ala Lys Trp Gly Val Ala Gly Pro Trp Tyr Leu Glu Val Trp Gly
100 105 110
caa ggc aca ctg gtt aca gtt tca agc gca cat cat tca gag gat ccg 384
Gln Gly Thr Leu Val Thr Val Ser Ser Ala His His Ser Glu Asp Pro
115 120 125
aca ggc ggt ggc tca cat cat cac cat cat cat taa 420
Thr Gly Gly Gly Ser His His His His His His
130 135
<210> 112
<211> 139
<212> PRT
<213> 未知(Unknown)
<220>
<223> 未知物种(Unknown species)
<400> 112
Ala Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly
1 5 10 15
Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Leu Thr Phe Ser Ser
20 25 30
Tyr Ala Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Phe
35 40 45
Val Ala Ala Ile Ser Trp Ser Gly Asp Ser Thr Tyr Tyr Ala Asp Ser
50 55 60
Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Val
65 70 75 80
Ser Leu Gln Met Asn Asn Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr
85 90 95
Cys Ala Lys Trp Gly Val Ala Gly Pro Trp Tyr Leu Glu Val Trp Gly
100 105 110
Gln Gly Thr Leu Val Thr Val Ser Ser Ala His His Ser Glu Asp Pro
115 120 125
Thr Gly Gly Gly Ser His His His His His His
130 135
<210> 113
<211> 417
<212> DNA
<213> 未知(Unknown)
<220>
<223> 未知物种(Unknown species)
<220>
<221> CDS
<222> (1)..(417)
<400> 113
gca gaa gtt caa ctg gtt gaa tca ggc gga ggc ctg gtc caa gca ggc 48
Ala Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Ala Gly
1 5 10 15
gga tca ctg aga ctg tca tgc gca gtt tca gga ccg aat ttt tca aat 96
Gly Ser Leu Arg Leu Ser Cys Ala Val Ser Gly Pro Asn Phe Ser Asn
20 25 30
tat gca atg ggc tgg ttt aga caa gtt ccg gga aga gaa aga ggc ttt 144
Tyr Ala Met Gly Trp Phe Arg Gln Val Pro Gly Arg Glu Arg Gly Phe
35 40 45
gtt gca gca att tca agc gga ggc tat aca ctg tat gaa gat tca gtt 192
Val Ala Ala Ile Ser Ser Gly Gly Tyr Thr Leu Tyr Glu Asp Ser Val
50 55 60
aaa gga cgc ttt aca atc tca agc gat cat gcg aaa aat acg gtc tat 240
Lys Gly Arg Phe Thr Ile Ser Ser Asp His Ala Lys Asn Thr Val Tyr
65 70 75 80
ctg caa atg aat gat ctg aaa ccg gaa gat aca gcg gtc tat tat tgc 288
Leu Gln Met Asn Asp Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys
85 90 95
gca aaa tgg gga gtt gca gga ccg tgg tat ctg gaa gtt tgg gga caa 336
Ala Lys Trp Gly Val Ala Gly Pro Trp Tyr Leu Glu Val Trp Gly Gln
100 105 110
ggc aca ctg gtt aca gtt tca tca gca cat cat tca gag gat ccg aca 384
Gly Thr Leu Val Thr Val Ser Ser Ala His His Ser Glu Asp Pro Thr
115 120 125
ggc ggt ggc tca cat cat cac cat cat cat taa 417
Gly Gly Gly Ser His His His His His His
130 135
<210> 114
<211> 138
<212> PRT
<213> 未知(Unknown)
<220>
<223> 未知物种(Unknown species)
<400> 114
Ala Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Ala Gly
1 5 10 15
Gly Ser Leu Arg Leu Ser Cys Ala Val Ser Gly Pro Asn Phe Ser Asn
20 25 30
Tyr Ala Met Gly Trp Phe Arg Gln Val Pro Gly Arg Glu Arg Gly Phe
35 40 45
Val Ala Ala Ile Ser Ser Gly Gly Tyr Thr Leu Tyr Glu Asp Ser Val
50 55 60
Lys Gly Arg Phe Thr Ile Ser Ser Asp His Ala Lys Asn Thr Val Tyr
65 70 75 80
Leu Gln Met Asn Asp Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys
85 90 95
Ala Lys Trp Gly Val Ala Gly Pro Trp Tyr Leu Glu Val Trp Gly Gln
100 105 110
Gly Thr Leu Val Thr Val Ser Ser Ala His His Ser Glu Asp Pro Thr
115 120 125
Gly Gly Gly Ser His His His His His His
130 135
<210> 115
<211> 417
<212> DNA
<213> 未知(Unknown)
<220>
<223> 未知物种(Unknown species)
<220>
<221> CDS
<222> (1)..(417)
<400> 115
gca gaa gtt caa ctg gtt gaa tca ggc gga ggc ctg gtc caa gca ggc 48
Ala Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Ala Gly
1 5 10 15
gga tca ctg aga ctg tca tgc gca gca tca ggc tca att ttt aga att 96
Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Ser Ile Phe Arg Ile
20 25 30
aat gca atg ggc tgg tat aga caa gca ccg gga aaa caa aga gaa ctg 144
Asn Ala Met Gly Trp Tyr Arg Gln Ala Pro Gly Lys Gln Arg Glu Leu
35 40 45
gtt gca aca att aca agc gga ggc tca aca aat tat gca gat tca gtt 192
Val Ala Thr Ile Thr Ser Gly Gly Ser Thr Asn Tyr Ala Asp Ser Val
50 55 60
aaa gga cgc ttt acg att agc aga gat aac gcg aaa aat acg ctg tac 240
Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Leu Tyr
65 70 75 80
ctg caa atg aat tca ctg aaa ccg gaa gat aca gcg gtc tat tat tgc 288
Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys
85 90 95
gca aaa tgg gga gtt gca gga ccg tgg tat ctg gaa gtt tgg gga caa 336
Ala Lys Trp Gly Val Ala Gly Pro Trp Tyr Leu Glu Val Trp Gly Gln
100 105 110
ggc aca ctg gtt aca gtt tca tca gca cat cat tca gag gat ccg aca 384
Gly Thr Leu Val Thr Val Ser Ser Ala His His Ser Glu Asp Pro Thr
115 120 125
ggc ggt ggc tca cat cat cac cat cat cat taa 417
Gly Gly Gly Ser His His His His His His
130 135
<210> 116
<211> 138
<212> PRT
<213> 未知(Unknown)
<220>
<223> 未知物种(Unknown species)
<400> 116
Ala Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Ala Gly
1 5 10 15
Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Ser Ile Phe Arg Ile
20 25 30
Asn Ala Met Gly Trp Tyr Arg Gln Ala Pro Gly Lys Gln Arg Glu Leu
35 40 45
Val Ala Thr Ile Thr Ser Gly Gly Ser Thr Asn Tyr Ala Asp Ser Val
50 55 60
Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Leu Tyr
65 70 75 80
Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys
85 90 95
Ala Lys Trp Gly Val Ala Gly Pro Trp Tyr Leu Glu Val Trp Gly Gln
100 105 110
Gly Thr Leu Val Thr Val Ser Ser Ala His His Ser Glu Asp Pro Thr
115 120 125
Gly Gly Gly Ser His His His His His His
130 135
<210> 117
<211> 420
<212> DNA
<213> 未知(Unknown)
<220>
<223> 未知物种(Unknown species)
<220>
<221> CDS
<222> (1)..(420)
<400> 117
gca gaa gtt caa ctg gtt gaa tca ggc gga ggc ctg gtc caa gca ggc 48
Ala Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Ala Gly
1 5 10 15
gga tca ctg aga ctg tca tgc gca aca tca ggc aga ccg ttt tca tca 96
Gly Ser Leu Arg Leu Ser Cys Ala Thr Ser Gly Arg Pro Phe Ser Ser
20 25 30
ctg gca atg ggc tgg ttt aga caa gca ccg gga aaa gaa aga gaa ttt 144
Leu Ala Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Phe
35 40 45
gtt gca agc att aat tgg agc gga gcg aac aca tat tat agc gat tca 192
Val Ala Ser Ile Asn Trp Ser Gly Ala Asn Thr Tyr Tyr Ser Asp Ser
50 55 60
gtt aaa gga cgc ttt acg att agc cgt gat aac gtc aaa aat acg gtc 240
Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Val Lys Asn Thr Val
65 70 75 80
tac ctg caa atg aat tca ctg aaa ccg gaa gat aca gcg gtc tat tat 288
Tyr Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr
85 90 95
tgc gca aaa tgg gga gtt gca gga ccg tgg tat ctg gaa gtt tgg gga 336
Cys Ala Lys Trp Gly Val Ala Gly Pro Trp Tyr Leu Glu Val Trp Gly
100 105 110
caa ggc aca ctg gtt aca gtt tca tca gca cat cat tca gag gat ccg 384
Gln Gly Thr Leu Val Thr Val Ser Ser Ala His His Ser Glu Asp Pro
115 120 125
aca ggc ggt ggc tca cat cat cac cat cat cat taa 420
Thr Gly Gly Gly Ser His His His His His His
130 135
<210> 118
<211> 139
<212> PRT
<213> 未知(Unknown)
<220>
<223> 未知物种(Unknown species)
<400> 118
Ala Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Ala Gly
1 5 10 15
Gly Ser Leu Arg Leu Ser Cys Ala Thr Ser Gly Arg Pro Phe Ser Ser
20 25 30
Leu Ala Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Phe
35 40 45
Val Ala Ser Ile Asn Trp Ser Gly Ala Asn Thr Tyr Tyr Ser Asp Ser
50 55 60
Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Val Lys Asn Thr Val
65 70 75 80
Tyr Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr
85 90 95
Cys Ala Lys Trp Gly Val Ala Gly Pro Trp Tyr Leu Glu Val Trp Gly
100 105 110
Gln Gly Thr Leu Val Thr Val Ser Ser Ala His His Ser Glu Asp Pro
115 120 125
Thr Gly Gly Gly Ser His His His His His His
130 135
<210> 119
<211> 423
<212> DNA
<213> 未知(Unknown)
<220>
<223> 未知物种(Unknown species)
<220>
<221> CDS
<222> (1)..(423)
<400> 119
gca gaa gtt caa ctg gtt gaa tca ggc gga ggc ctg gtt caa gca ggc 48
Ala Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Ala Gly
1 5 10 15
gat tca ctg aga ctg tca tgc gca aca tca ggc ctt aca ttt tca aat 96
Asp Ser Leu Arg Leu Ser Cys Ala Thr Ser Gly Leu Thr Phe Ser Asn
20 25 30
ttt gca atg ggc tgg ttt aga caa gca ccg gga aaa gaa aga gaa ttt 144
Phe Ala Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Phe
35 40 45
gtt gca gca att aat cgc aat ggc gga agc gtt tat tat caa ccg tca 192
Val Ala Ala Ile Asn Arg Asn Gly Gly Ser Val Tyr Tyr Gln Pro Ser
50 55 60
gtt aaa gga cgc ttt aca att agc aga gac aac gcg aaa aat acg ctg 240
Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Leu
65 70 75 80
tat ctg caa atg aat tca ctg aaa ccg gaa gat acg gca gtc tat tat 288
Tyr Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr
85 90 95
tgc gca aaa tgg gga gtt gca gga ccg tgg tat ctg gaa gtt tgg gga 336
Cys Ala Lys Trp Gly Val Ala Gly Pro Trp Tyr Leu Glu Val Trp Gly
100 105 110
caa ggc aca ctg gtt aca gtt tca tca gaa ccg aaa aca ccg aaa ccg 384
Gln Gly Thr Leu Val Thr Val Ser Ser Glu Pro Lys Thr Pro Lys Pro
115 120 125
caa agc gga ggc gga tca cat cat cac cat cat cat taa 423
Gln Ser Gly Gly Gly Ser His His His His His His
130 135 140
<210> 120
<211> 140
<212> PRT
<213> 未知(Unknown)
<220>
<223> 未知物种(Unknown species)
<400> 120
Ala Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Ala Gly
1 5 10 15
Asp Ser Leu Arg Leu Ser Cys Ala Thr Ser Gly Leu Thr Phe Ser Asn
20 25 30
Phe Ala Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Phe
35 40 45
Val Ala Ala Ile Asn Arg Asn Gly Gly Ser Val Tyr Tyr Gln Pro Ser
50 55 60
Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Leu
65 70 75 80
Tyr Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr
85 90 95
Cys Ala Lys Trp Gly Val Ala Gly Pro Trp Tyr Leu Glu Val Trp Gly
100 105 110
Gln Gly Thr Leu Val Thr Val Ser Ser Glu Pro Lys Thr Pro Lys Pro
115 120 125
Gln Ser Gly Gly Gly Ser His His His His His His
130 135 140
<210> 121
<211> 414
<212> DNA
<213> 未知(Unknown)
<220>
<223> 未知物种(Unknown species)
<220>
<221> CDS
<222> (1)..(414)
<400> 121
gca gaa gtt caa ctg gtt gaa tca ggc gga ggc ctg gtc caa gca ggc 48
Ala Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Ala Gly
1 5 10 15
gga tca ctg aga ctg tca tgc aca gca tca ggc aca aga gtt aga ctg 96
Gly Ser Leu Arg Leu Ser Cys Thr Ala Ser Gly Thr Arg Val Arg Leu
20 25 30
tca ggc atg ggc tgg ttt aga caa gca cct ggc gga caa aga gaa atg 144
Ser Gly Met Gly Trp Phe Arg Gln Ala Pro Gly Gly Gln Arg Glu Met
35 40 45
gtt gca aca atg aca gat agc aac acg tat tat cgc gat tca gtc aaa 192
Val Ala Thr Met Thr Asp Ser Asn Thr Tyr Tyr Arg Asp Ser Val Lys
50 55 60
gga cgc ttt aca att tca cgc gat aat gcg aaa aac acg ctg tat ctg 240
Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Leu Tyr Leu
65 70 75 80
caa atg aat tca ctg aaa ccg gaa gat acg gca gtc tat tat tgc gca 288
Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys Ala
85 90 95
aaa tgg gga gtt gca gga ccg tgg tat ctg gaa gtt tgg gga caa ggc 336
Lys Trp Gly Val Ala Gly Pro Trp Tyr Leu Glu Val Trp Gly Gln Gly
100 105 110
aca ctg gtt aca gtt tca tca gca cat cat tca gag gat ccg aca ggc 384
Thr Leu Val Thr Val Ser Ser Ala His His Ser Glu Asp Pro Thr Gly
115 120 125
ggt ggc tca cat cat cac cat cat cat taa 414
Gly Gly Ser His His His His His His
130 135
<210> 122
<211> 137
<212> PRT
<213> 未知(Unknown)
<220>
<223> 未知物种(Unknown species)
<400> 122
Ala Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Ala Gly
1 5 10 15
Gly Ser Leu Arg Leu Ser Cys Thr Ala Ser Gly Thr Arg Val Arg Leu
20 25 30
Ser Gly Met Gly Trp Phe Arg Gln Ala Pro Gly Gly Gln Arg Glu Met
35 40 45
Val Ala Thr Met Thr Asp Ser Asn Thr Tyr Tyr Arg Asp Ser Val Lys
50 55 60
Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Leu Tyr Leu
65 70 75 80
Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys Ala
85 90 95
Lys Trp Gly Val Ala Gly Pro Trp Tyr Leu Glu Val Trp Gly Gln Gly
100 105 110
Thr Leu Val Thr Val Ser Ser Ala His His Ser Glu Asp Pro Thr Gly
115 120 125
Gly Gly Ser His His His His His His
130 135
<210> 123
<211> 420
<212> DNA
<213> 未知(Unknown)
<220>
<223> 未知物种(Unknown species)
<220>
<221> CDS
<222> (1)..(420)
<400> 123
gca gaa gtt caa ctg gtt gaa tca ggc gga ggc ctg gtc caa gca ggc 48
Ala Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Ala Gly
1 5 10 15
gga tca ctg aga ctg tca tgc gca gca tca ggc aga aca ttt tca aca 96
Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Thr Phe Ser Thr
20 25 30
tat aat gtc ggc tgg ttt aga cag tca ccg gga aaa gaa aga gaa ttt 144
Tyr Asn Val Gly Trp Phe Arg Gln Ser Pro Gly Lys Glu Arg Glu Phe
35 40 45
gtt gca gca att gca tgg gga ggc gga gat aca ggc tat gca gat tca 192
Val Ala Ala Ile Ala Trp Gly Gly Gly Asp Thr Gly Tyr Ala Asp Ser
50 55 60
gtt aaa gga cgc ttt aca atc agc cgt gac aaa agc aaa aat acg gtc 240
Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Lys Ser Lys Asn Thr Val
65 70 75 80
tat ctg caa atg gat tca ctg aaa ccg gaa gat aca gca gtc tat tat 288
Tyr Leu Gln Met Asp Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr
85 90 95
tgc gca aaa tgg gga gtt gca gga ccg tgg tat ctg gaa gtt tgg gga 336
Cys Ala Lys Trp Gly Val Ala Gly Pro Trp Tyr Leu Glu Val Trp Gly
100 105 110
caa ggc aca ctg gtt aca gtt tca tca gca cat cat tca gag gat ccg 384
Gln Gly Thr Leu Val Thr Val Ser Ser Ala His His Ser Glu Asp Pro
115 120 125
aca ggc ggt ggc tca cat cat cac cat cat cat taa 420
Thr Gly Gly Gly Ser His His His His His His
130 135
<210> 124
<211> 139
<212> PRT
<213> 未知(Unknown)
<220>
<223> 未知物种(Unknown species)
<400> 124
Ala Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Ala Gly
1 5 10 15
Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Thr Phe Ser Thr
20 25 30
Tyr Asn Val Gly Trp Phe Arg Gln Ser Pro Gly Lys Glu Arg Glu Phe
35 40 45
Val Ala Ala Ile Ala Trp Gly Gly Gly Asp Thr Gly Tyr Ala Asp Ser
50 55 60
Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Lys Ser Lys Asn Thr Val
65 70 75 80
Tyr Leu Gln Met Asp Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr
85 90 95
Cys Ala Lys Trp Gly Val Ala Gly Pro Trp Tyr Leu Glu Val Trp Gly
100 105 110
Gln Gly Thr Leu Val Thr Val Ser Ser Ala His His Ser Glu Asp Pro
115 120 125
Thr Gly Gly Gly Ser His His His His His His
130 135
<210> 125
<211> 357
<212> DNA
<213> 未知(Unknown)
<220>
<223> 未知物种(Unknown species)
<220>
<221> CDS
<222> (1)..(357)
<400> 125
gca gaa gtt caa ctg gtt gaa tca ggc gga ggc ctg gtt caa cct ggc 48
Ala Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly
1 5 10 15
gga tca ctg aga ctg tca tgc gtt ggc ttt ccg aga tat ctg tca atg 96
Gly Ser Leu Arg Leu Ser Cys Val Gly Phe Pro Arg Tyr Leu Ser Met
20 25 30
tca tgg ctg aga caa gca ccg gga aaa gaa ctg gaa tgg gtt ttt aca 144
Ser Trp Leu Arg Gln Ala Pro Gly Lys Glu Leu Glu Trp Val Phe Thr
35 40 45
atc agc aaa gac aac gcg aaa aac aca gtc tat ctg caa atg aat tca 192
Ile Ser Lys Asp Asn Ala Lys Asn Thr Val Tyr Leu Gln Met Asn Ser
50 55 60
ctg aaa ccg gaa gat aca gcg gtc tat tat tgc aat gca aga aga tca 240
Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys Asn Ala Arg Arg Ser
65 70 75 80
aca gca ctt ggc gca aga gat tat tgg gga caa ggc aca ctg gtt aca 288
Thr Ala Leu Gly Ala Arg Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr
85 90 95
gtt tca tca gaa ccg aaa aca ccg aaa ccg caa agc gga ggc gga agc 336
Val Ser Ser Glu Pro Lys Thr Pro Lys Pro Gln Ser Gly Gly Gly Ser
100 105 110
cat cac cat cat cat cat taa 357
His His His His His His
115
<210> 126
<211> 118
<212> PRT
<213> 未知(Unknown)
<220>
<223> 未知物种(Unknown species)
<400> 126
Ala Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly
1 5 10 15
Gly Ser Leu Arg Leu Ser Cys Val Gly Phe Pro Arg Tyr Leu Ser Met
20 25 30
Ser Trp Leu Arg Gln Ala Pro Gly Lys Glu Leu Glu Trp Val Phe Thr
35 40 45
Ile Ser Lys Asp Asn Ala Lys Asn Thr Val Tyr Leu Gln Met Asn Ser
50 55 60
Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys Asn Ala Arg Arg Ser
65 70 75 80
Thr Ala Leu Gly Ala Arg Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr
85 90 95
Val Ser Ser Glu Pro Lys Thr Pro Lys Pro Gln Ser Gly Gly Gly Ser
100 105 110
His His His His His His
115
<210> 127
<211> 402
<212> DNA
<213> 未知(Unknown)
<220>
<223> 未知物种(Unknown species)
<220>
<221> CDS
<222> (1)..(402)
<400> 127
gca gaa gtt caa ctg gtt gaa tca ggc gga ggc gtt gca aga cct ggc 48
Ala Glu Val Gln Leu Val Glu Ser Gly Gly Gly Val Ala Arg Pro Gly
1 5 10 15
gga tca ctg aca ctg tca tgc gtt gca tca ggc tca caa ttt ggc gca 96
Gly Ser Leu Thr Leu Ser Cys Val Ala Ser Gly Ser Gln Phe Gly Ala
20 25 30
ggc ttt aga gat atg gca tgg tat aga caa gat ccg gga aaa gaa cgc 144
Gly Phe Arg Asp Met Ala Trp Tyr Arg Gln Asp Pro Gly Lys Glu Arg
35 40 45
gaa cat gtt gca ggc att aca aaa gat ggc ttt aca gat tat gcg aac 192
Glu His Val Ala Gly Ile Thr Lys Asp Gly Phe Thr Asp Tyr Ala Asn
50 55 60
agc gtc aaa ggc cgt ttt aca att tca cgc gat aat gcg aaa aac acg 240
Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr
65 70 75 80
gtc tat ctg caa atg aat tca ctg tct ccg gaa ggc aca gca atc tat 288
Val Tyr Leu Gln Met Asn Ser Leu Ser Pro Glu Gly Thr Ala Ile Tyr
85 90 95
tat tgc aat ctg aga ctg gtt tgg gga caa ggc aca caa gtt aca gtt 336
Tyr Cys Asn Leu Arg Leu Val Trp Gly Gln Gly Thr Gln Val Thr Val
100 105 110
tca tca gaa ccg aaa aca ccg aaa ccg caa agc gga ggc gga agc cat 384
Ser Ser Glu Pro Lys Thr Pro Lys Pro Gln Ser Gly Gly Gly Ser His
115 120 125
cac cat cat cat cat taa 402
His His His His His
130
<210> 128
<211> 133
<212> PRT
<213> 未知(Unknown)
<220>
<223> 未知物种(Unknown species)
<400> 128
Ala Glu Val Gln Leu Val Glu Ser Gly Gly Gly Val Ala Arg Pro Gly
1 5 10 15
Gly Ser Leu Thr Leu Ser Cys Val Ala Ser Gly Ser Gln Phe Gly Ala
20 25 30
Gly Phe Arg Asp Met Ala Trp Tyr Arg Gln Asp Pro Gly Lys Glu Arg
35 40 45
Glu His Val Ala Gly Ile Thr Lys Asp Gly Phe Thr Asp Tyr Ala Asn
50 55 60
Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr
65 70 75 80
Val Tyr Leu Gln Met Asn Ser Leu Ser Pro Glu Gly Thr Ala Ile Tyr
85 90 95
Tyr Cys Asn Leu Arg Leu Val Trp Gly Gln Gly Thr Gln Val Thr Val
100 105 110
Ser Ser Glu Pro Lys Thr Pro Lys Pro Gln Ser Gly Gly Gly Ser His
115 120 125
His His His His His
130
<210> 129
<211> 426
<212> DNA
<213> 未知(Unknown)
<220>
<223> 未知物种(Unknown species)
<220>
<221> CDS
<222> (1)..(426)
<400> 129
gca gaa gtt caa ctg gtt gaa tca ggc gga ggc ctg gtt caa gca ggc 48
Ala Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Ala Gly
1 5 10 15
gaa tca ctg aca ctg tca tgc gca gca tca ggc aga aga ttt ggc tca 96
Glu Ser Leu Thr Leu Ser Cys Ala Ala Ser Gly Arg Arg Phe Gly Ser
20 25 30
caa ttt atg ggc tgg ttt aga caa gca caa ggc aaa gca aga gaa tat 144
Gln Phe Met Gly Trp Phe Arg Gln Ala Gln Gly Lys Ala Arg Glu Tyr
35 40 45
gtt gca gca att agc ctg aat ggc gtt aga aaa tac tat gcg gaa tca 192
Val Ala Ala Ile Ser Leu Asn Gly Val Arg Lys Tyr Tyr Ala Glu Ser
50 55 60
gtc aaa gga cgc ttt aca att tca cgc gat aat gcg aaa aac acg gtc 240
Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Val
65 70 75 80
tat ctg caa atg aat tca ctg aaa ccg gaa gat aca gcg gtc tat tat 288
Tyr Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr
85 90 95
tca cat gca ccg gca att ctg aca gca aca aga cgc ctg gat tat tgg 336
Ser His Ala Pro Ala Ile Leu Thr Ala Thr Arg Arg Leu Asp Tyr Trp
100 105 110
gga caa ggc aca caa gtt aca gtt tca tca gaa ccg aaa aca ccg aaa 384
Gly Gln Gly Thr Gln Val Thr Val Ser Ser Glu Pro Lys Thr Pro Lys
115 120 125
ccg caa agc gga ggc gga tca cat cat cac cat cat cat taa 426
Pro Gln Ser Gly Gly Gly Ser His His His His His His
130 135 140
<210> 130
<211> 141
<212> PRT
<213> 未知(Unknown)
<220>
<223> 未知物种(Unknown species)
<400> 130
Ala Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Ala Gly
1 5 10 15
Glu Ser Leu Thr Leu Ser Cys Ala Ala Ser Gly Arg Arg Phe Gly Ser
20 25 30
Gln Phe Met Gly Trp Phe Arg Gln Ala Gln Gly Lys Ala Arg Glu Tyr
35 40 45
Val Ala Ala Ile Ser Leu Asn Gly Val Arg Lys Tyr Tyr Ala Glu Ser
50 55 60
Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Val
65 70 75 80
Tyr Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr
85 90 95
Ser His Ala Pro Ala Ile Leu Thr Ala Thr Arg Arg Leu Asp Tyr Trp
100 105 110
Gly Gln Gly Thr Gln Val Thr Val Ser Ser Glu Pro Lys Thr Pro Lys
115 120 125
Pro Gln Ser Gly Gly Gly Ser His His His His His His
130 135 140
<210> 131
<211> 396
<212> DNA
<213> 未知(Unknown)
<220>
<223> 未知物种(Unknown species)
<220>
<221> CDS
<222> (1)..(396)
<400> 131
gca gaa gtt caa ctg gtt gaa tca ggc gga ggc ctg gtt caa cct ggc 48
Ala Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly
1 5 10 15
gga tca ctg aca ctg gca aga aca gca aca ggc att gca ttt tca aca 96
Gly Ser Leu Thr Leu Ala Arg Thr Ala Thr Gly Ile Ala Phe Ser Thr
20 25 30
cat gtt atg ggc tgg tat aga caa gca ccg gga aaa caa aga gaa ctg 144
His Val Met Gly Trp Tyr Arg Gln Ala Pro Gly Lys Gln Arg Glu Leu
35 40 45
gtt gca gca att tca agc gga ggc aca ccg tat tat gtt gat tca gtt 192
Val Ala Ala Ile Ser Ser Gly Gly Thr Pro Tyr Tyr Val Asp Ser Val
50 55 60
aaa gga cgc ttt acg atc agc aga gat aat gcg aaa aat aca gtt agc 240
Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Val Ser
65 70 75 80
ctg cag atg aat tca ctg aaa ccg gaa gat aca gca gtc tac tat tgc 288
Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys
85 90 95
aat ttt cgc gtt tat tgg gga caa ggc aca caa gtt aca gtt tca tca 336
Asn Phe Arg Val Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser
100 105 110
gaa ccg aaa aca ccg aaa ccg caa tct ggc gga gga tca cat cat cac 384
Glu Pro Lys Thr Pro Lys Pro Gln Ser Gly Gly Gly Ser His His His
115 120 125
cat cat cat taa 396
His His His
130
<210> 132
<211> 131
<212> PRT
<213> 未知(Unknown)
<220>
<223> 未知物种(Unknown species)
<400> 132
Ala Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly
1 5 10 15
Gly Ser Leu Thr Leu Ala Arg Thr Ala Thr Gly Ile Ala Phe Ser Thr
20 25 30
His Val Met Gly Trp Tyr Arg Gln Ala Pro Gly Lys Gln Arg Glu Leu
35 40 45
Val Ala Ala Ile Ser Ser Gly Gly Thr Pro Tyr Tyr Val Asp Ser Val
50 55 60
Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Val Ser
65 70 75 80
Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys
85 90 95
Asn Phe Arg Val Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser
100 105 110
Glu Pro Lys Thr Pro Lys Pro Gln Ser Gly Gly Gly Ser His His His
115 120 125
His His His
130
<210> 133
<211> 420
<212> DNA
<213> 未知(Unknown)
<220>
<223> 未知物种(Unknown species)
<220>
<221> CDS
<222> (1)..(420)
<400> 133
gca gaa gtt caa ctg gtt gaa tca ggc gga ggc ctg gca caa gca ggc 48
Ala Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Ala Gln Ala Gly
1 5 10 15
gga tca ctg aga ctg tca tgc gca gca tca ggc aga aca ttt tca gca 96
Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Thr Phe Ser Ala
20 25 30
tat gtt atg ggc tgg ttt aga caa gca ccg gga aaa gaa aga gtt ccg 144
Tyr Val Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Val Pro
35 40 45
gtt gca gca att aat aga ctg ggc agc att att aga tat gcg gat tca 192
Val Ala Ala Ile Asn Arg Leu Gly Ser Ile Ile Arg Tyr Ala Asp Ser
50 55 60
gtt aaa gga cgc ttt aca att agc cgt gat cgc aca aaa aat acg gtc 240
Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Arg Thr Lys Asn Thr Val
65 70 75 80
tat ctg caa atg aat aac ctg aaa ccg gaa gat aca gca gtt tat tct 288
Tyr Leu Gln Met Asn Asn Leu Lys Pro Glu Asp Thr Ala Val Tyr Ser
85 90 95
tgc gca gca agc gca aga ccg gga tca tca tgg gat tat tgg gga caa 336
Cys Ala Ala Ser Ala Arg Pro Gly Ser Ser Trp Asp Tyr Trp Gly Gln
100 105 110
ggc aca caa gtt aca gtt tca tca gaa ccg aaa aca ccg aaa ccg caa 384
Gly Thr Gln Val Thr Val Ser Ser Glu Pro Lys Thr Pro Lys Pro Gln
115 120 125
agc gga ggc gga agc cat cac cat cat cat cat taa 420
Ser Gly Gly Gly Ser His His His His His His
130 135
<210> 134
<211> 139
<212> PRT
<213> 未知(Unknown)
<220>
<223> 未知物种(Unknown species)
<400> 134
Ala Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Ala Gln Ala Gly
1 5 10 15
Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Thr Phe Ser Ala
20 25 30
Tyr Val Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Val Pro
35 40 45
Val Ala Ala Ile Asn Arg Leu Gly Ser Ile Ile Arg Tyr Ala Asp Ser
50 55 60
Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Arg Thr Lys Asn Thr Val
65 70 75 80
Tyr Leu Gln Met Asn Asn Leu Lys Pro Glu Asp Thr Ala Val Tyr Ser
85 90 95
Cys Ala Ala Ser Ala Arg Pro Gly Ser Ser Trp Asp Tyr Trp Gly Gln
100 105 110
Gly Thr Gln Val Thr Val Ser Ser Glu Pro Lys Thr Pro Lys Pro Gln
115 120 125
Ser Gly Gly Gly Ser His His His His His His
130 135
<210> 135
<211> 417
<212> DNA
<213> 未知(Unknown)
<220>
<223> 未知物种(Unknown species)
<220>
<221> CDS
<222> (1)..(417)
<400> 135
gca gaa gtt caa ctg gtt gaa tca ggc gga ggc ctg gtt caa aca ggc 48
Ala Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Thr Gly
1 5 10 15
gca tca ctg aga att tca tgc gca gtt aca ggc caa aca ctg aaa aaa 96
Ala Ser Leu Arg Ile Ser Cys Ala Val Thr Gly Gln Thr Leu Lys Lys
20 25 30
tac aca gtt ggc tgg cat aga caa gca ccg gga aaa gaa aga gaa ttt 144
Tyr Thr Val Gly Trp His Arg Gln Ala Pro Gly Lys Glu Arg Glu Phe
35 40 45
gtt gca gtt att ggc tgg aca tca ggc gat aca cat tat gca gca tca 192
Val Ala Val Ile Gly Trp Thr Ser Gly Asp Thr His Tyr Ala Ala Ser
50 55 60
gtt aaa gga cgc ttt aca att tca aga ggc aat gcg aaa aac gcc atg 240
Val Lys Gly Arg Phe Thr Ile Ser Arg Gly Asn Ala Lys Asn Ala Met
65 70 75 80
tat ctg caa atg aat aat ccg aaa ccg gaa gat aca gcg gtc tat tat 288
Tyr Leu Gln Met Asn Asn Pro Lys Pro Glu Asp Thr Ala Val Tyr Tyr
85 90 95
tgc aat gca gat gtt gtc tgg ctg aaa aaa gat tat tgg gga caa ggc 336
Cys Asn Ala Asp Val Val Trp Leu Lys Lys Asp Tyr Trp Gly Gln Gly
100 105 110
aca caa gtt aca gtt tca tca gaa ccg aaa aca ccg aaa ccg caa agc 384
Thr Gln Val Thr Val Ser Ser Glu Pro Lys Thr Pro Lys Pro Gln Ser
115 120 125
gga ggc gga tca cat cat cac cat cat cat taa 417
Gly Gly Gly Ser His His His His His His
130 135
<210> 136
<211> 138
<212> PRT
<213> 未知(Unknown)
<220>
<223> 未知物种(Unknown species)
<400> 136
Ala Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Thr Gly
1 5 10 15
Ala Ser Leu Arg Ile Ser Cys Ala Val Thr Gly Gln Thr Leu Lys Lys
20 25 30
Tyr Thr Val Gly Trp His Arg Gln Ala Pro Gly Lys Glu Arg Glu Phe
35 40 45
Val Ala Val Ile Gly Trp Thr Ser Gly Asp Thr His Tyr Ala Ala Ser
50 55 60
Val Lys Gly Arg Phe Thr Ile Ser Arg Gly Asn Ala Lys Asn Ala Met
65 70 75 80
Tyr Leu Gln Met Asn Asn Pro Lys Pro Glu Asp Thr Ala Val Tyr Tyr
85 90 95
Cys Asn Ala Asp Val Val Trp Leu Lys Lys Asp Tyr Trp Gly Gln Gly
100 105 110
Thr Gln Val Thr Val Ser Ser Glu Pro Lys Thr Pro Lys Pro Gln Ser
115 120 125
Gly Gly Gly Ser His His His His His His
130 135
<210> 137
<211> 429
<212> DNA
<213> 未知(Unknown)
<220>
<223> 未知物种(Unknown species)
<220>
<221> CDS
<222> (1)..(429)
<400> 137
gca gaa gtt caa ctg gtt gaa tca ggc gga ggc tca gtt caa cct ggc 48
Ala Glu Val Gln Leu Val Glu Ser Gly Gly Gly Ser Val Gln Pro Gly
1 5 10 15
gga tca ctg att ctg tca tgc gca gtt tca ggc cat att ttc ttt tat 96
Gly Ser Leu Ile Leu Ser Cys Ala Val Ser Gly His Ile Phe Phe Tyr
20 25 30
ccg ctg gca tgg tat aga caa gca ccg gga aaa caa aga gaa ctg gtt 144
Pro Leu Ala Trp Tyr Arg Gln Ala Pro Gly Lys Gln Arg Glu Leu Val
35 40 45
gca agc att tca aca aca ggc aga aca cat tat gca gat gca gtt aaa 192
Ala Ser Ile Ser Thr Thr Gly Arg Thr His Tyr Ala Asp Ala Val Lys
50 55 60
gga cgc ttt aca atc ttt cgc gat aat gtt aaa aga aca gtt agc ctt 240
Gly Arg Phe Thr Ile Phe Arg Asp Asn Val Lys Arg Thr Val Ser Leu
65 70 75 80
cgc atg aat tca ctg aaa ccg gaa gat aca gca gtc tat tat tgc aaa 288
Arg Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys Lys
85 90 95
aca gca aat atg ggc tca agc tgg tgc cat gat att tca ttt aca tca 336
Thr Ala Asn Met Gly Ser Ser Trp Cys His Asp Ile Ser Phe Thr Ser
100 105 110
tgg gga caa ggc aca caa gtt aca gtt tca tca gaa ccg aaa aca ccg 384
Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser Glu Pro Lys Thr Pro
115 120 125
aaa ccg caa agc gga ggc gga agc cat cac cat cat cat cat taa 429
Lys Pro Gln Ser Gly Gly Gly Ser His His His His His His
130 135 140
<210> 138
<211> 142
<212> PRT
<213> 未知(Unknown)
<220>
<223> 未知物种(Unknown species)
<400> 138
Ala Glu Val Gln Leu Val Glu Ser Gly Gly Gly Ser Val Gln Pro Gly
1 5 10 15
Gly Ser Leu Ile Leu Ser Cys Ala Val Ser Gly His Ile Phe Phe Tyr
20 25 30
Pro Leu Ala Trp Tyr Arg Gln Ala Pro Gly Lys Gln Arg Glu Leu Val
35 40 45
Ala Ser Ile Ser Thr Thr Gly Arg Thr His Tyr Ala Asp Ala Val Lys
50 55 60
Gly Arg Phe Thr Ile Phe Arg Asp Asn Val Lys Arg Thr Val Ser Leu
65 70 75 80
Arg Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys Lys
85 90 95
Thr Ala Asn Met Gly Ser Ser Trp Cys His Asp Ile Ser Phe Thr Ser
100 105 110
Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser Glu Pro Lys Thr Pro
115 120 125
Lys Pro Gln Ser Gly Gly Gly Ser His His His His His His
130 135 140

Claims (10)

1.一种重链抗体的重链可变区的制造方法，其特征在于：
包括对芽孢杆菌属菌进行培养的步骤，
该芽孢杆菌属菌导入有编码重链抗体的重链可变区的基因，并且缺损细胞外蛋白酶。
2.如权利要求1所述的方法，其特征在于：
所述细胞外蛋白酶为选自epr、wprA、bpr、vpr、aprE、aprX和与它们相当的基因所编码的细胞外蛋白酶中的至少1种。
3.如权利要求1或2所述的方法，其特征在于：
所述重链抗体的重链可变区由序列号2、4、6、8、10、12、14、16、18、20、22、24、26、28、30、32、34、36、38、64、66、68、70、72、74、76、78、80、82、84、86、88、90、92、94、96、98、100、102、104、106、108、110、112、114、116、118、120、122、124、126、128、130、132、134、136或138的氨基酸序列、或与该序列至少85％相同的氨基酸序列构成。
4.如权利要求1～3中任一项所述的方法，其特征在于：
所述芽孢杆菌属菌为枯草杆菌或其突变株。
5.如权利要求1～4中任一项所述的方法，其特征在于：
缺损所述细胞外蛋白酶的芽孢杆菌属菌为编码该细胞外蛋白酶的基因缺失或钝化后的芽孢杆菌属菌。
6.一种芽孢杆菌属菌，其特征在于：
导入有编码重链抗体的重链可变区的基因，并且缺损细胞外蛋白酶。
7.如权利要求6所述的芽孢杆菌属菌，其特征在于：
所述细胞外蛋白酶为选自epr、wprA、bpr、vpr、aprE、aprX和与它们相当的基因所编码的细胞外蛋白酶中的至少1种。
8.如权利要求6或7所述的芽孢杆菌属菌，其特征在于：
所述重链抗体的重链可变区由序列号2、4、6、8、10、12、14、16、18、20、22、24、26、28、30、32、34、36、38、64、66、68、70、72、74、76、78、80、82、84、86、88、90、92、94、96、98、100、102、104、106、108、110、112、114、116、118、120、122、124、126、128、130、132、134、136或138的氨基酸序列、或与该序列至少85％相同的氨基酸序列构成。
9.如权利要求6～8中任一项所述的芽孢杆菌属菌，其特征在于：
所述芽孢杆菌属菌为枯草杆菌或其突变株。
10.如权利要求6～9中任一项所述的芽孢杆菌属菌，其特征在于：
编码所述细胞外蛋白酶的基因缺失或钝化。
CN202080094749.0A 2020-01-27 2020-12-25 重链抗体的重链可变区的制造方法 Pending CN115038791A (zh)

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