CN115024326A - Slow-release plant growth regulator and preparation method and application thereof - Google Patents

Slow-release plant growth regulator and preparation method and application thereof Download PDF

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CN115024326A
CN115024326A CN202210969736.4A CN202210969736A CN115024326A CN 115024326 A CN115024326 A CN 115024326A CN 202210969736 A CN202210969736 A CN 202210969736A CN 115024326 A CN115024326 A CN 115024326A
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plant growth
duckweed
weight
parts
growth regulator
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CN115024326B (en
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褚刚
梁宸
朱明全
杨桂梅
刘汇
周明军
杨华潘
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Shandong Epson Agricultural Development Co ltd
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    • A—HUMAN NECESSITIES
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    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
    • A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
    • A01N43/38—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings condensed with carbocyclic rings
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/08—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing solids as carriers or diluents
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    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/42—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing within the same carbon skeleton a carboxylic group or a thio analogue, or a derivative thereof, and a carbon atom having only two bonds to hetero atoms with at the most one bond to halogen, e.g. keto-carboxylic acids
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/22—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom rings with more than six members
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/40—Liliopsida [monocotyledons]
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P21/00—Plant growth regulators
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05B—PHOSPHATIC FERTILISERS
    • C05B1/00—Superphosphates, i.e. fertilisers produced by reacting rock or bone phosphates with sulfuric or phosphoric acid in such amounts and concentrations as to yield solid products directly
    • C05B1/02—Superphosphates
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05B—PHOSPHATIC FERTILISERS
    • C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/40—Mixtures of one or more fertilisers with additives not having a specially fertilising activity for affecting fertiliser dosage or release rate; for affecting solubility
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    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture

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Abstract

The invention provides a slow-release plant growth regulator and a preparation method and application thereof, belonging to the technical field of plant growth regulators 2 Dispersing the microspheres in water, adding nutrient substances, a duckweed percolation extract, a duckweed fermentation extract and a plant growth promoting composition, concentrating, naturally drying to obtain plant growth regulator loaded microspheres, and further coating the surfaces of the plant growth regulator loaded microspheres with a polydopamine layer to obtain the slow-release plant growth regulator. The slow-release plant growth regulator prepared by the invention can release the plant growth regulator for a long time, the product of the invention is not required to be sprayed for multiple times, the action time of the plant growth regulator is prolonged, the slow-release plant growth regulator is safe and non-toxic, and the slow-release plant growth regulator can be cleaned up after being strongly cleaned, so that the plant growth can be effectively promoted, a good regulating effect is achieved, and the slow-release plant growth regulator has a wide application prospect.

Description

Slow-release plant growth regulator and preparation method and application thereof
Technical Field
The invention relates to the technical field of plant growth regulators, in particular to a slow-release plant growth regulator and a preparation method and application thereof.
Background
In recent years, in order to obtain high yield of crops, vegetables and fruits, pesticides and chemical fertilizers are used in excess. The chemical fertilizer and pesticide residues in agricultural products exceed the standard, soil and water body pollution is caused, and a series of environmental damage and ecological disasters are caused. Seriously threatens human survival and development.
In order to solve the problems, a plurality of biological organic fertilizers and pesticides are developed at home and abroad. The organic mixture formed during the dry distillation of plant material has a variety of biological effects. It was used in japan as a feed additive, a soil deodorant and an organic fertilizer starter at the end of the last century. And compared with a systematic research, the influence of the substances on the germination of vegetable seeds and the growth and development of seedlings is researched. The research of China shows that the seedling damping off and other diseases can be effectively prevented. The organic mixture is used for treating seeds, cuttings and root systems of the Holland and the marigold, and can improve the germination rate of the seeds, the rooting rate of the cuttings and the number of the root systems of seedlings. It is also used as a starter culture for biofertilizers. However, the use effect varies greatly from place to place, and even the opposite is reported. The reason is that the prior formulations or fertilizers have the following disadvantages: the plant distillate is not produced according to specific processes such as standard temperature, pressure, processing time and the like, the obtained total mixture is obtained, and interaction among some mixtures weakens the effect on plants; the total nutrient content is not strengthened and can not meet the nutritional requirement of plants; the plant nutrient solution cannot be produced according to the classification of raw materials, and different plant raw materials have different components and different effects on plants. Although some products exist at home and abroad and have a long application history, the requirements of Chinese economic development and ecological environment construction are difficult to adapt.
The plant growth regulator is one kind of matter for regulating plant growth and development, and includes artificially synthesized compound and natural plant hormone extracted from organism. In plant cultivation, the plant growth regulator with lower concentration can play different roles of promoting, delaying or inhibiting and the like, thereby achieving the purposes of rooting, sprouting, yield increase, stress resistance improvement and the like. Meanwhile, the plant growth regulator can also effectively solve the difficult problems in the cultivation technology, and especially plays an active role in breaking seed dormancy, promoting plant growth, overcoming the limitation of crop production environment and improving harvest and storage conditions.
Plant growth regulators are exogenous non-nutritive chemicals that are usually delivered to the site of action in the plant and at very low concentrations can promote or inhibit certain parts of its life process, leading to the development of a plant that meets the needs of humans. Each plant growth regulator has specific application, and the application requirements are quite strict, and the specific effect on target plants can be generated only under specific application conditions (including external factors). Often, varying concentrations will produce opposite results, e.g., promoting at low concentrations and inhibiting at high concentrations. Plant growth regulators have many uses, which vary depending on the species and target plant. For example: controlling germination and dormancy; promoting rooting; promoting cell elongation and division; controlling lateral buds or tillering; controlling the plant type (short, strong and lodging-resistant); controlling the sex of flowering or male and female, and inducing seedless fruits; thinning flowers and fruits and controlling fruit dropping; controlling the shape or maturity of the fruit; enhancing stress resistance (disease resistance, drought resistance, salinity resistance and freezing resistance); enhancing the fertilizer absorption capacity; increasing sugar or altering acidity; the flavor and color are improved; promoting latex or resin secretion; defoliation or estimation (facilitating mechanical harvesting); preservation, and the like. Some plant growth regulators are herbicides when used in high concentrations, and some herbicides also have growth regulating effects at low concentrations. The traditional plant growth regulator has high liquid medicine residue, great pollution and low utilization rate, and does not meet the development requirement of ecological agriculture.
Generally, the growth characteristics of plants can be significantly changed after spraying the plant growth regulator, but the effect is lost after the growth regulator is drenched or degraded by rain, and thus, the plant growth regulator needs to be sprayed several times, which is inconvenient in use. If a plant growth regulator capable of slowly releasing is developed, the plant growth regulator has good adhesion to relevant parts of plants and can play a role in long-term release, so that the effect of regulating and controlling the plant growth for a long time is achieved, and the plant growth regulator has obvious practical significance.
Duckweed is an uncommon Chinese medicine, has the effects of dispelling wind heat, promoting eruption and inducing diuresis, and is mainly used for treating rheumatism and paralysis, edema, wind heat hidden eruption, hematemesis and blood interest, aphthous stomatitis and the like in clinic. Pharmacological experiments show that the duckweed has the effects of diuresis, antibiosis, antipyresis and cardiotonic. The duckweed is one of plants with higher growth speed in the world, and is rich in natural plant growth promoters.
Disclosure of Invention
The invention aims to provide a slow-release plant growth regulator and a preparation method and application thereof, the slow-release plant growth regulator can be released fixedly for a long time, the product is not required to be sprayed and applied for many times, the slow-release plant growth regulator is released for a long time, the action time of the plant growth regulator is prolonged, the slow-release plant growth regulator is safe and non-toxic, and after strong cleaning, the slow-release plant growth regulator can be cleaned, so that the plant growth can be effectively promoted, a good regulating effect is achieved, and the slow-release plant growth regulator has a wide application prospect.
The invention provides a preparation method of a slow-release plant growth regulator, which comprises the steps of adding aminosilane into water with a pore-forming agent and a surfactant, and stirring for reaction to obtain porous hollow SiO 2 MicrospheresDispersing in water, adding nutrient substances, a duckweed percolation extract, a duckweed fermentation extract and a plant growth promoting composition, concentrating, naturally drying to obtain plant growth regulator-loaded microspheres, and further coating the surfaces with a polydopamine layer to obtain a slow-release plant growth regulator;
the nutrient substances consist of nitrogenous fertilizer, phosphate fertilizer and potash fertilizer;
the plant growth promoting composition consists of indolebutyric acid, brassinosteroid and jasmonic acid.
As a further improvement of the invention, the method comprises the following steps:
s1, porous hollow SiO 2 Preparing microspheres: dissolving a first pore-forming agent and a surfactant in water, dropwise adding aminosilane, stirring for reaction, centrifugally washing, and spray-drying to obtain porous hollow SiO 2 Microspheres;
s2, preparation of nutrient substances: uniformly mixing a nitrogenous fertilizer, a phosphate fertilizer and a potash fertilizer to obtain a nutrient substance;
s3, preparing a duckweed diacolation extract: cleaning duckweed, drying, pulverizing to obtain duckweed powder, percolating with ethanol solution, mixing percolates after complete percolation, recovering ethanol under reduced pressure, drying the concentrate to volatilize solvent to obtain duckweed percolate extract, and reserving the extracted duckweed powder for later use;
s4, preparing a duckweed fermentation extract: adding the extracted duckweed powder obtained in the step S3 into water, inoculating activated zymocyte liquid, fermenting, filtering, and freeze-drying to obtain a duckweed fermentation extract;
s5, preparing a plant growth promoting composition: uniformly mixing indolebutyric acid, brassinosteroid and jasmonic acid to obtain a plant growth promoting composition;
s6, preparation of the plant growth regulator loaded microspheres: dispersing the nutrient substance obtained in step S2, the percolating extract of duckweed obtained in step S3, the fermented extract of duckweed obtained in step S4, and the plant growth promoting composition obtained in step S5 in water, and adding the porous hollow SiO obtained in step S1 2 Dispersing the microspheres uniformly, concentrating to remove the solvent, and naturally drying to obtain the plant growth regulator loaded microspheres;
s7, preparation of the slow-release plant growth regulator: and (4) uniformly dispersing the loaded plant growth regulator microspheres prepared in the step (S6) in water, adding a catalyst solution, dopamine hydrochloride and a second pore-forming agent, stirring for reaction, filtering, and washing with water to obtain the slow-release plant growth regulator.
In a further improvement of the present invention, the aminosilane in step S1 is at least one selected from the group consisting of 3-aminopropyltriethoxysilane, γ -aminopropyltrimethoxysilane, N- β (aminoethyl) - γ -aminopropylmethyldimethoxysilane, N- β (aminoethyl) - γ -aminopropyltriethoxysilane, N- β (aminoethyl) - γ -aminopropylmethyldiethoxysilane, and divinyltriaminopropyltrimethoxysilane; the first pore-forming agent is polyoxyethylene sorbitan fatty acid ester or polyethylene glycol octyl phenyl ether; the surfactant is selected from at least one of sodium dodecyl benzene sulfonate, sodium dodecyl sulfate, sodium hexadecyl benzene sulfonate, sodium hexadecyl sulfate, sodium octadecyl sulfonate and sodium octadecyl benzene sulfonate; the mass ratio of the first pore-forming agent to the surfactant to the aminosilane is 1-2:2-4: 70-100.
As a further improvement of the present invention, in step S2, the nitrogen fertilizer is at least one selected from urea, ammonium nitrate, ammonium sulfate, ammonium chloride, ammonium bicarbonate, ammonia water, sodium nitrate, calcium ammonium nitrate and ammonium sulfate nitrate; the phosphate fertilizer is selected from at least one of sodium phosphate, calcium superphosphate, triple superphosphate, enriched superphosphate and ammonium phosphate; the potash fertilizer is at least one of potassium chloride, potassium sulfate and potassium nitrate; the mass ratio of the nitrogenous fertilizer to the phosphate fertilizer to the potash fertilizer is 3-5:2-4: 0.5-1; in the step S3, the solid-to-liquid ratio of the duckweed powder to the ethanol solution is 1:5-10 g/mL; the ethanol content in the ethanol solution is 50-70 wt%; the percolation extraction time is 1-3 h.
As a further improvement of the invention, the solid-to-liquid ratio of the duckweed powder and water after extraction in step S4 is 1:3-5 g/mL; the zymocyte is bacillus subtilis, acetic acid bacteria and lactobacillus plantarum; said activationThe bacteria content of the zymocyte liquid is 10 8 -10 9 cfu/mL; the inoculation amounts of the bacillus subtilis, the acetic acid bacteria and the lactobacillus plantarum are respectively 1-3%, 2-4% and 2-3%; the fermentation conditions are 35-40 deg.C, 50-70% humidity, and 3-7% CO 2 Fermenting for 48-72h under the volume content.
As a further improvement of the invention, the mass ratio of the indole butyric acid, the brassinosteroid and the jasmonic acid in the step S5 is 7-10:2-5: 1-2; in the step S6, the mass ratio of the nutrient substances, the duckweed percolation extract, the duckweed fermentation extract and the plant growth promoting composition is 10-20:5-12:7-10: 0.1-0.3.
As a further improvement of the method, in step S7, the mass ratio of the plant growth regulator-loaded microspheres, the catalyst solution, the dopamine hydrochloride and the second pore-forming agent is 20-50:2-5:30-60: 1-2; the catalyst solution is Co 2+ A Tris-HCl solution with pH value of 5.5-6.5 and content of 3-5wt%, wherein the second pore-foaming agent is at least one selected from cetyl trimethyl ammonium bromide, oxyethylene-oxypropylene triblock copolymer PEO20-PPO70-PEO20, PEO106-PPO70-PEO 106.
As a further improvement of the invention, the method specifically comprises the following steps:
s1, porous hollow SiO 2 Preparing microspheres: dissolving 1-2 parts by weight of first pore-foaming agent and 2-4 parts by weight of surfactant in 200 parts by weight of water, dropwise adding 70-100 parts by weight of aminosilane, stirring for reaction, centrifugally washing, and spray drying to obtain porous hollow SiO 2 Microspheres;
s2, preparing nutrient substances: uniformly mixing 3-5 parts by weight of nitrogenous fertilizer, 2-4 parts by weight of phosphate fertilizer and 0.5-1 part by weight of potash fertilizer to obtain nutrient substances;
s3, preparing a duckweed diacolation extract: cleaning duckweed, drying, crushing to obtain duckweed powder, percolating and extracting for 1-3h by using 50-70wt% of ethanol solution, wherein the solid-to-liquid ratio of the duckweed powder to the 50-70wt% of ethanol solution is 1:5-10g/mL, merging percolate after complete percolation, recovering ethanol under reduced pressure, drying and volatilizing a solvent of a concentrate to obtain a duckweed percolation extract, and reserving the extracted duckweed powder for later use;
s4, preparing a duckweed fermentation extract: will be provided withAdding the extracted duckweed powder obtained in the step S3 into water, wherein the solid-to-liquid ratio of the extracted duckweed powder to the water is 1:3-5g/mL, and the inoculation bacteria content is 10 8 -10 9 The cfu/mL activated zymocyte liquid is respectively bacillus subtilis liquid, acetic acid bacteria liquid and lactobacillus plantarum liquid, the inoculation amount is respectively 1-3%, 2-4% and 2-3%, and 3-7% CO is obtained at 35-40 ℃ and 50-70% humidity 2 Fermenting for 48-72h under the volume content, filtering, and freeze-drying to obtain herba Spirodelae fermented extract;
s5, preparing a plant growth promoting composition: uniformly mixing 7-10 parts by weight of indolebutyric acid, 2-5 parts by weight of brassinosteroids and 1-2 parts by weight of jasmonic acid to obtain a plant growth promoting composition;
s6, preparation of the plant growth regulator loaded microspheres: dispersing 10-20 parts by weight of the nutrient substance prepared in step S2, 5-12 parts by weight of the duckweed diacolation extract prepared in step S3, 7-10 parts by weight of the duckweed fermentation extract prepared in step S4, and 0.1-0.3 part by weight of the plant growth promoting composition prepared in step S5 in 100 parts by weight of water, and adding 100 parts by weight of the porous hollow SiO prepared in step S1 2 Dispersing the microspheres uniformly, concentrating to remove the solvent, and naturally drying to obtain plant growth regulator-loaded microspheres;
s7, preparation of the slow-release plant growth regulator: uniformly dispersing 20-50 parts by weight of the plant growth regulator-loaded microspheres prepared in the step S6 in 200 parts by weight of water, and adding 2-5 parts by weight of Co 2+ The slow-release plant growth regulator is prepared by stirring and reacting 3-5wt% of Tris-HCl solution with the pH value of 5.5-6.5, 30-60 parts of dopamine hydrochloride and 1-2 parts of second pore-forming agent by weight, filtering and washing with water.
The invention further protects the slow-release plant growth regulator prepared by the preparation method.
The invention further protects the application of the slow-release plant growth regulator in promoting the plant growth for a long time.
The invention has the following beneficial effects: the invention firstly prepares a porous hollow SiO 2 The microspheres are prepared by dripping aminosilane and surfactant into water containing pore-forming agent and surfactantInitially insoluble in water and dispersed into tiny droplets during agitation. With the development of the reaction process, the amino group part of the aminosilane is protonated to form an amphiphilic molecule, so that on one hand, the silane liquid drop is stabilized, and on the other hand, the protonated amino group provides an alkaline environment to catalyze the silane part to perform a sol-gel reaction. With the consumption of the internal silane and the existence of the surfactant, the structure of the hollow microsphere with better shape is obtained, and on the microsphere interface, a large number of macropores are formed due to the existence of the pore-forming agent, so that the porous hollow SiO is obtained 2 And (3) microspheres.
The added plant growth promoting composition comprises indolebutyric acid, brassinosteroid and jasmonic acid, and has good effect of promoting plant growth, wherein the indolebutyric acid has good effect of inducing formation of root protomer, promoting cell differentiation and division, facilitating generation of new roots and differentiation of vascular bundle systems, promoting formation of adventitious roots of cuttings, improving photosynthetic efficiency, promoting accumulation of photosynthetic products and promoting cell division; brassinosteroids high-activity substances extracted from rape pollen and capable of promoting plant stem elongation and cell division transmit signals through cell membrane surface receptors, and can strongly induce growth and differentiation; jasmonic acid also has certain effects on promoting the formation of plant epidermal hair, inhibits the growth and development of the plant epidermal hair by causing the change of plant gene expression, resists the adverse stress of the external environment, improves the content of secondary metabolites and improves the stress resistance of the plant, so the added plant growth regulator composition can promote the growth of the plant, improves the stress resistance of the plant, obviously improves the growth and development level of the plant and has the synergistic effect;
the duckweed is rich in polysaccharide, flavonoid, orientin, vitexin, linolenic acid, luteolin and the like, most of nutrient substances are dissolved out through percolation and fermentation extraction, rich nutrient substances are obtained, the duckweed is one of the plants with the fastest growing speed in the world and contains rich substances for promoting plant growth, and the substances and the nutrient substances have synergistic effect, so that the nutrition in the plants can be timely supplemented, the plant productivity and the resistance to the external adverse environment can be improved by regulating the physiological state of the plants, improving the physiological function, enhancing the plant permeability regulating capability, promoting the formation of chloroplast, improving the utilization efficiency of plant moisture and sunlight and the stability of cell membranes, and enhancing the early warning and reaction capability of the plants to the adverse environment, thereby improving the effects of resisting drought, salt and cold of the plants and inhibiting the occurrence of plant diseases and insect pests.
The preparation of the slow-release plant growth regulator is added with nutrient substances, including nitrogen fertilizer, phosphate fertilizer and potash fertilizer, applied to relevant parts of plants, beneficial to the absorption of the plants through surface capillary channels and providing nutrients required for promoting the growth of the plants, thereby ensuring the nutrient supply while the plants grow rapidly and ensuring the healthy growth of the plants;
the invention is characterized in that nutrient substances, duckweed diacolation extract, duckweed fermentation extract and plant growth promoting composition are fixedly carried in porous hollow SiO 2 The microspheres contain rich plant growth promoting substances, the pores on the surfaces of the microspheres can continuously release the plant growth regulator, and the surfaces of the prepared microspheres loaded with the plant growth regulator are coated with a polydopamine layer, so that the release rate of the plant growth regulator can be further slowed down, meanwhile, the polydopamine is rich in hydroxyl, sulfydryl, amino, carboxyl and other groups, the prepared slow-release plant growth regulator can be promoted to be fixed on the surfaces of relevant parts of plants, the plant growth regulator can be released for a long time in a fixed manner, the product disclosed by the invention does not need to be sprayed for multiple times, the slow-release plant growth regulator is released for a long time, the action aging of the plant growth regulator is prolonged, the slow-release plant growth regulator is safe and non-toxic, and can be cleaned up after being strongly cleaned, so that the plant growth can be effectively promoted, has good regulating effect and wide application prospect.
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In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the embodiments or the description of the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 shows a porous hollow SiO prepared in example 1 of the present invention 2 TEM images of the microspheres;
FIG. 2 is an SEM photograph of the sustained-release plant growth regulator prepared in example 1 of the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Bacillus subtilis with the content of 1000 hundred million cfu/g is purchased from Guangxi agricultural Prov. Biotechnology Limited; acetic acid bacteria with the content of 30 hundred million cfu/g are purchased from Wuhanpeng Bio-technology limited; lactobacillus plantarum with the content of 50 hundred million cfu/g is purchased from Weifang Ruichi Biotechnology Limited; duckweed is the whole plant of duckweed, purchased from Taiji group Shaping Dachun pharmacy, Chongqing Dayun Shuiko Chinese medicinal material company, Sichuan of origin. Indolebutyric acid, CAS: 133-32-4, purity greater than 99%, available from merck chemical technology (shanghai) ltd; brassinosteroids, CAS: 474-60-2 with purity of more than 95 percent, which is purchased from Shanghai Yuanmu biotechnology limited; jasmonic acid, purity greater than 98%, CAS: 77026-92-7, available from Yangxi pharmaceutical technology, Inc., Hubei.
Example 1
The embodiment provides a preparation method of a slow-release plant growth regulator, which specifically comprises the following steps:
s1, porous hollow SiO 2 Preparing microspheres: dissolving 1 weight part of polyoxyethylene sorbitan fatty acid ester and 2 weight parts of sodium dodecyl sulfate in 200 weight parts of water, dropwise adding 70 weight parts of gamma-aminopropyltriethoxysilane, stirring at the rotating speed of 1000r/min, reacting at 50 ℃ for 4 hours, centrifuging at 3000r/min for 15 minutes, washing with deionized water, and spray-drying to obtain the porous hollow SiO 2 Microspheres; FIG. 1 shows the prepared polypeptideSiO with hollow hole 2 The TEM image of the microsphere shows that the microsphere has a hollow structure.
S2, preparation of nutrient substances: uniformly mixing 3 parts by weight of ammonium nitrate, 2 parts by weight of sodium phosphate and 0.5 part by weight of potassium nitrate to obtain a nutrient substance;
s3, preparing a duckweed diacolation extract: cleaning duckweed, drying at 70 ℃ for 2h, crushing to obtain duckweed powder, percolating and extracting for 1h by using 50wt% of ethanol solution, wherein the solid-to-liquid ratio of the duckweed powder to the 50wt% of ethanol solution is 1:5g/mL, merging percolate after complete percolation, recovering ethanol under reduced pressure, drying volatile solvent of a concentrate to obtain a duckweed percolation extract, and reserving the extracted duckweed powder for later use;
s4, preparing a duckweed fermentation extract: adding the extracted duckweed powder obtained in the step S3 into water, wherein the solid-to-liquid ratio of the extracted duckweed powder to the water is 1:3g/mL, inoculating activated bacillus subtilis liquid, activated acetic acid bacteria liquid and activated lactobacillus plantarum bacteria liquid, and the bacteria content of each bacteria liquid is 10 8 cfu/mL, inoculum size 1%, 2%, respectively, 3% CO at 35 deg.C under 50% humidity 2 Fermenting for 48h under the volume content, filtering, and freeze-drying to obtain a duckweed fermented extract;
s5, preparing a plant growth promoting composition: uniformly mixing 7 parts by weight of indolebutyric acid, 2 parts by weight of brassinosteroid and 1 part by weight of jasmonic acid to obtain a plant growth promoting composition;
s6, preparation of the plant growth regulator loaded microspheres: dispersing 10 parts by weight of the nutrient substance obtained in step S2, 5 parts by weight of the percolating extract of duckweed obtained in step S3, 7 parts by weight of the fermented extract of duckweed obtained in step S4, and 0.1 part by weight of the plant growth promoting composition obtained in step S5 in 100 parts by weight of water, and adding 100 parts by weight of the porous hollow SiO obtained in step S1 2 Carrying out ultrasonic dispersion for 1h at 1000W on microspheres, concentrating to remove a solvent, and naturally drying to obtain plant growth regulator-loaded microspheres;
s7, preparation of the slow-release plant growth regulator: adding 20 parts by weight of the plant growth regulator-loaded microspheres prepared in the step S6 into 200 parts by weight of water, ultrasonically dispersing for 1 hour at 1000W, and adding 2 parts by weight of Co 2+ Tris-HC of pH 5.5 with a content of 3wt%l solution, 30 parts by weight of dopamine hydrochloride and 1 part by weight of hexadecyl trimethyl ammonium bromide, stirring at the rotating speed of 300r/min, reacting for 4 hours at 45 ℃, filtering and washing to obtain the slow-release plant growth regulator, and as shown in a figure 2, a SEM picture of the prepared slow-release plant growth regulator shows, a large number of pores are formed on the surface of the slow-release plant growth regulator, so that the slow release of the plant growth regulator is facilitated.
Example 2
The embodiment provides a preparation method of a slow-release plant growth regulator, which specifically comprises the following steps:
s1, porous hollow SiO 2 Preparing microspheres: dissolving 2 parts by weight of polyethylene glycol octyl phenyl ether and 4 parts by weight of sodium hexadecyl sulfate in 200 parts by weight of water, dropwise adding 100 parts by weight of diethylenetriaminopropyltrimethoxysilane, stirring at the rotating speed of 1000r/min, reacting at 50 ℃ for 8 hours, centrifuging at 3000r/min for 15 minutes, washing with deionized water, and spray-drying to obtain the porous hollow SiO 2 Microspheres;
s2, preparing nutrient substances: uniformly mixing 5 parts by weight of urea, 4 parts by weight of calcium superphosphate and 1 part by weight of potassium chloride to obtain a nutrient substance;
s3, preparing a duckweed percolation extract: cleaning duckweed, drying at 70 ℃ for 2h, crushing to obtain duckweed powder, percolating and extracting for 3h by using 70wt% of ethanol solution, wherein the solid-to-liquid ratio of the duckweed powder to the 70wt% of ethanol solution is 1:10g/mL, merging percolate after complete percolation, recovering ethanol under reduced pressure, drying volatile solvent of a concentrate to obtain a duckweed percolation extract, and reserving the extracted duckweed powder for later use;
s4, preparing a duckweed fermentation extract: adding the extracted duckweed powder obtained in the step S3 into water, wherein the solid-to-liquid ratio of the extracted duckweed powder to the water is 1:5g/mL, inoculating activated bacillus subtilis liquid, activated acetic acid bacteria liquid and activated lactobacillus plantarum bacteria liquid, and the bacteria content of each bacteria liquid is 10 9 cfu/mL, inoculum size 3%, 4%, 3%, respectively, 7% CO at 40 deg.C under 70% humidity 2 Fermenting for 72h under the volume content, filtering, and freeze-drying to obtain herba Spirodelae fermented extract;
s5, preparing a plant growth promoting composition: uniformly mixing 10 parts by weight of indolebutyric acid, 5 parts by weight of brassinosteroid and 2 parts by weight of jasmonic acid to obtain a plant growth promoting composition;
s6, preparation of the plant growth regulator loaded microspheres: dispersing 20 parts by weight of the nutrient substance obtained in step S2, 12 parts by weight of the percolating extract of duckweed obtained in step S3, 10 parts by weight of the fermented extract of duckweed obtained in step S4, and 0.3 part by weight of the plant growth promoting composition obtained in step S5 in 100 parts by weight of water, and adding 100 parts by weight of the porous hollow SiO solid obtained in step S1 2 Carrying out ultrasonic dispersion for 1h at 1000W on microspheres, concentrating to remove a solvent, and naturally drying to obtain plant growth regulator-loaded microspheres;
s7, preparation of the slow-release plant growth regulator: adding 50 parts by weight of the plant growth regulator-loaded microspheres prepared in the step S6 into 200 parts by weight of water, ultrasonically dispersing for 1 hour at 1000W, and adding 5 parts by weight of Co 2+ The slow-release plant growth regulator is prepared by stirring a Tris-HCl solution with the pH value of 6.5 and the content of 5wt%, 60 parts by weight of dopamine hydrochloride and 2 parts by weight of an oxyethylene-oxypropylene triblock copolymer PEO20-PPO70-PEO20 at the rotating speed of 300r/min, reacting for 4 hours at the temperature of 45 ℃, filtering and washing with water.
Example 3
The embodiment provides a preparation method of a slow-release plant growth regulator, which specifically comprises the following steps:
s1, porous hollow SiO 2 Preparing microspheres: dissolving 1.5 parts by weight of polyoxyethylene sorbitan fatty acid ester and 3 parts by weight of sodium octadecylbenzene sulfonate in 200 parts by weight of water, dropwise adding 85 parts by weight of N-beta (aminoethyl) -gamma-aminopropyltrimethoxysilane, stirring at the rotating speed of 1000r/min, reacting at 50 ℃ for 6 hours, centrifuging at 3000r/min for 15min, washing with deionized water, and spray-drying to obtain porous hollow SiO 2 Microspheres;
s2, preparation of nutrient substances: uniformly mixing 4 parts by weight of ammonium sulfate nitrate, 3 parts by weight of enriched calcium superphosphate and 0.7 part by weight of potassium sulfate to obtain a nutrient substance;
s3, preparing a duckweed diacolation extract: cleaning duckweed, drying at 70 ℃ for 2h, crushing to obtain duckweed powder, percolating and extracting for 2h by using 60wt% of ethanol solution, wherein the solid-to-liquid ratio of the duckweed powder to the 60wt% of ethanol solution is 1:7g/mL, merging percolates after complete percolation, recovering ethanol under reduced pressure, drying and volatilizing a solvent in a concentrate to obtain a duckweed percolation extract, and reserving the extracted duckweed powder;
s4, preparing a duckweed fermentation extract: adding the duckweed powder obtained in the step S3 into water, wherein the solid-to-liquid ratio of the duckweed powder to the water is 1:4g/mL, inoculating activated bacillus subtilis liquid, acetic acid bacteria liquid and lactobacillus plantarum liquid, and the bacteria content of each bacterium liquid is 10 9 cfu/mL, inoculum size of 2%, 3%, 2.5%, 5% CO at 37 deg.C under 60% humidity 2 Fermenting for 56h under the volume content, filtering, and freeze-drying to obtain herba Spirodelae fermented extract;
s5, preparing a plant growth promoting composition: uniformly mixing 8.5 parts by weight of indolebutyric acid, 3.5 parts by weight of brassinosteroid and 1.5 parts by weight of jasmonic acid to obtain a plant growth promoting composition;
s6, preparation of the plant growth regulator loaded microspheres: dispersing 15 parts by weight of the nutrient substance obtained in step S2, 8 parts by weight of the percolating extract of duckweed obtained in step S3, 8.5 parts by weight of the fermented extract of duckweed obtained in step S4, and 0.2 part by weight of the plant growth promoting composition obtained in step S5 in 100 parts by weight of water, and adding 100 parts by weight of the porous hollow SiO solid obtained in step S1 2 Carrying out ultrasonic dispersion on microspheres for 1h at 1000W, concentrating to remove a solvent, and naturally drying to obtain plant growth regulator-loaded microspheres;
s7, preparation of the slow-release plant growth regulator: adding 35 parts by weight of the plant growth regulator-loaded microspheres prepared in the step S6 into 200 parts by weight of water, ultrasonically dispersing for 1 hour at 1000W, and adding 3.5 parts by weight of Co 2+ The slow-release plant growth regulator is prepared by stirring 4wt% of Tris-HCl solution with the pH value of 6, 45 parts of dopamine hydrochloride and 1.5 parts of ethylene oxide-propylene oxide triblock copolymer PEO106-PPO70-PEO106 at the rotating speed of 300r/min, reacting for 4 hours at 45 ℃, filtering and washing with water.
Example 4
Compared with example 3, indole butyric acid is not added in step S5, and other conditions are not changed.
The method comprises the following specific steps:
s5, preparing a plant growth promoting composition: uniformly mixing 12 parts by weight of brassinosteroids and 1.5 parts by weight of jasmonic acid to obtain the plant growth promoting composition.
Example 5
Compared with example 3, no brassinosteroid was added in step S5, and the other conditions were not changed.
The method comprises the following specific steps:
s5, preparing a plant growth promoting composition: uniformly mixing 12 parts by weight of indolebutyric acid and 1.5 parts by weight of jasmonic acid to obtain the plant growth promoting composition.
Example 6
Compared with example 3, no bacillus subtilis solution was added in step S4, and the other conditions were not changed.
The method comprises the following specific steps:
s4, preparing a duckweed fermentation extract: adding the extracted duckweed powder obtained in the step S3 into water, wherein the solid-to-liquid ratio of the extracted duckweed powder to the water is 1:4g/mL, inoculating activated acetic acid bacteria liquid and lactobacillus plantarum bacteria liquid, and the bacteria content of each group of bacteria liquid is 10 9 cfu/mL, inoculum size 3%, 2.5%, respectively, 5% CO at 37 deg.C under 60% humidity 2 Fermenting for 56h under the volume content, filtering, and freeze-drying to obtain the fermented extract of duckweed.
Example 7
In step S4, no acetic acid bacteria solution was added, compared to example 3, and the other conditions were not changed.
The method comprises the following specific steps:
s4, preparing a duckweed fermentation extract: adding the extracted duckweed powder obtained in the step S3 into water, wherein the solid-to-liquid ratio of the extracted duckweed powder to the water is 1:4g/mL, inoculating activated bacillus subtilis liquid and lactobacillus plantarum liquid, and the bacterial content of each group of the bacterial liquid is 10 9 cfu/mL, inoculum size 2%, 2.5%, 5% CO at 37 deg.C under 60% humidity 2 Fermenting for 56h under the volume content, filtering, and freeze-drying to obtain the duckweed fermented extract.
Example 8
In step S4, the lactobacillus plantarum strain solution was not added, as compared to example 3, and the other conditions were not changed.
The method comprises the following specific steps:
s4, preparing a duckweed fermentation extract: adding the extracted duckweed powder obtained in the step S3 into water, wherein the solid-to-liquid ratio of the extracted duckweed powder to the water is 1:4g/mL, inoculating activated bacillus subtilis liquid and acetic acid bacteria liquid, and the bacteria content of each group of bacteria liquid is 10 9 cfu/mL, inoculum size 2%, 3%, respectively, 5% CO at 37 deg.C under 60% humidity 2 Fermenting for 56h under the volume content, filtering, and freeze-drying to obtain the fermented extract of duckweed.
Comparative example 1
Compared with example 3, the nutrient substance of S2 was not added, and other conditions were not changed.
The method comprises the following specific steps:
s1, porous hollow SiO 2 Preparing microspheres: dissolving 1.5 parts by weight of polyoxyethylene sorbitan fatty acid ester and 3 parts by weight of sodium octadecylbenzene sulfonate in 200 parts by weight of water, dropwise adding 85 parts by weight of N-beta (aminoethyl) -gamma-aminopropyltrimethoxysilane, stirring at the rotating speed of 1000r/min, reacting at 50 ℃ for 6 hours, centrifuging at 3000r/min for 15min, washing with deionized water, and spray-drying to obtain porous hollow SiO 2 Microspheres;
s2, preparing a duckweed diacolation extract: cleaning duckweed, drying at 70 ℃ for 2h, crushing to obtain duckweed powder, percolating and extracting for 2h by using 60wt% of ethanol solution, wherein the solid-to-liquid ratio of the duckweed powder to the 60wt% of ethanol solution is 1:7g/mL, merging percolate after complete percolation, recovering ethanol under reduced pressure, drying volatile solvent of a concentrate to obtain a duckweed percolation extract, and reserving the extracted duckweed powder for later use;
s3, preparing a duckweed fermentation extract: adding the extracted duckweed powder obtained in the step S2 into water, wherein the solid-to-liquid ratio of the extracted duckweed powder to the water is 1:4g/mL, inoculating activated bacillus subtilis liquid, activated acetic acid bacteria liquid and activated lactobacillus plantarum bacteria liquid, and the bacteria content of each bacteria liquid is 10 9 cfu/mL, inoculum size of 2%, 3%, 2.5%, 5% CO at 37 deg.C under 60% humidity 2 Fermenting for 56h under the volume content, filtering, and freeze-drying to obtain herba Spirodelae fermented extract;
s4, preparing a plant growth promoting composition: uniformly mixing 8.5 parts by weight of indolebutyric acid, 3.5 parts by weight of brassinosteroid and 1.5 parts by weight of jasmonic acid to obtain a plant growth promoting composition;
s5, preparing the plant growth regulator loaded microspheres: dispersing 8 parts by weight of the diacolation extract of duckweed obtained in step S2, 8.5 parts by weight of the fermentation extract of duckweed obtained in step S3, and 0.2 part by weight of the plant growth promoting composition obtained in step S4 in 100 parts by weight of water, and adding 100 parts by weight of the porous hollow SiO solid obtained in step S1 2 Carrying out ultrasonic dispersion for 1h at 1000W on microspheres, concentrating to remove a solvent, and naturally drying to obtain plant growth regulator-loaded microspheres;
s6, preparation of the slow-release plant growth regulator: adding 35 parts by weight of the plant growth regulator-loaded microspheres prepared in the step S5 into 200 parts by weight of water, ultrasonically dispersing for 1 hour at 1000W, and adding 3.5 parts by weight of Co 2+ The slow-release plant growth regulator is prepared by stirring 4wt% of Tris-HCl solution with the pH value of 6, 45 parts of dopamine hydrochloride and 1.5 parts of ethylene oxide-propylene oxide triblock copolymer PEO106-PPO70-PEO106 at the rotating speed of 300r/min, reacting for 4 hours at 45 ℃, filtering and washing with water.
Comparative example 2
Compared with example 3, the lemna minor percolation extract in step S3 was not added, and other conditions were not changed.
The method comprises the following specific steps:
s1, porous hollow SiO 2 Preparing microspheres: dissolving 1.5 parts by weight of polyoxyethylene sorbitan fatty acid ester and 3 parts by weight of sodium octadecylbenzene sulfonate in 200 parts by weight of water, dropwise adding 85 parts by weight of N-beta (aminoethyl) -gamma-aminopropyltrimethoxysilane, stirring at the rotating speed of 1000r/min, reacting at 50 ℃ for 6 hours, centrifuging at 3000r/min for 15min, washing with deionized water, and spray-drying to obtain porous hollow SiO 2 Microspheres;
s2, preparation of nutrient substances: uniformly mixing 4 parts by weight of ammonium sulfate nitrate, 3 parts by weight of enriched calcium superphosphate and 0.7 part by weight of potassium sulfate to obtain a nutrient substance;
s3, preparing a duckweed fermentation extract: cleaning herba Spirodelae, drying at 70 deg.C for 2h, pulverizing to obtain herba Spirodelae powder, adding into water at solid-to-liquid ratio of 1:4g/mL, inoculating activated Bacillus subtilis budBacillus liquid, acetic acid bacteria liquid and plant lactobacillus liquid, wherein the bacteria content in each liquid is 10 9 cfu/mL, inoculum size of 2%, 3%, 2.5%, 5% CO at 37 deg.C under 60% humidity 2 Fermenting for 56h under the volume content, filtering, and freeze-drying to obtain herba Spirodelae fermented extract;
s4, preparing a plant growth promoting composition: uniformly mixing 8.5 parts by weight of indolebutyric acid, 3.5 parts by weight of brassinosteroid and 1.5 parts by weight of jasmonic acid to obtain a plant growth promoting composition;
s5, preparing the plant growth regulator loaded microspheres: dispersing 15 parts by weight of the nutrient substance prepared in step S2, 8.5 parts by weight of the fermented extract of duckweed prepared in step S3, and 0.2 part by weight of the plant growth promoting composition prepared in step S4 in 100 parts by weight of water, and adding 100 parts by weight of the porous hollow SiO prepared in step S1 2 Carrying out ultrasonic dispersion for 1h at 1000W on microspheres, concentrating to remove a solvent, and naturally drying to obtain plant growth regulator-loaded microspheres;
s6, preparation of the slow-release plant growth regulator: adding 35 parts by weight of the plant growth regulator-loaded microspheres prepared in the step S5 into 200 parts by weight of water, ultrasonically dispersing for 1 hour at 1000W, and adding 3.5 parts by weight of Co 2+ The slow-release plant growth regulator is prepared by stirring 4wt% of Tris-HCl solution with the pH value of 6, 45 parts of dopamine hydrochloride and 1.5 parts of ethylene oxide-propylene oxide triblock copolymer PEO106-PPO70-PEO106 at the rotating speed of 300r/min, reacting for 4 hours at 45 ℃, filtering and washing with water.
Comparative example 3
Compared with example 3, the duckweed fermented extract in step S4 was not added, and other conditions were not changed.
The method comprises the following specific steps:
s1, porous hollow SiO 2 Preparing microspheres: dissolving 1.5 parts by weight of polyoxyethylene sorbitan fatty acid ester and 3 parts by weight of sodium octadecylbenzene sulfonate in 200 parts by weight of water, dropwise adding 85 parts by weight of N-beta (aminoethyl) -gamma-aminopropyltrimethoxysilane, stirring at the rotating speed of 1000r/min, reacting at 50 ℃ for 6 hours, centrifuging at 3000r/min for 15min, washing with deionized water, and spray-drying to obtain porous hollow SiO 2 Microspheres;
s2, preparation of nutrient substances: uniformly mixing 4 parts by weight of ammonium sulfate nitrate, 3 parts by weight of enriched calcium superphosphate and 0.7 part by weight of potassium sulfate to obtain a nutrient substance;
s3, preparing a duckweed diacolation extract: cleaning duckweed, drying at 70 ℃ for 2h, crushing to obtain duckweed powder, percolating and extracting for 2h by using 60wt% of ethanol solution, wherein the solid-to-liquid ratio of the duckweed powder to the 60wt% of ethanol solution is 1:7g/mL, merging percolate after complete percolation, recovering ethanol under reduced pressure, and drying volatile solvent of a concentrate to obtain a duckweed percolation extract;
s4, preparing a plant growth promoting composition: uniformly mixing 8.5 parts by weight of indolebutyric acid, 3.5 parts by weight of brassinosteroid and 1.5 parts by weight of jasmonic acid to obtain a plant growth promoting composition;
s5, preparing the plant growth regulator loaded microspheres: dispersing 15 parts by weight of the nutrient substance prepared in step S2, 8 parts by weight of the duckweed diacolation extract prepared in step S3, and 0.2 part by weight of the plant growth promoting composition prepared in step S5 in 100 parts by weight of water, and adding 100 parts by weight of the porous hollow SiO prepared in step S1 2 Carrying out ultrasonic dispersion for 1h at 1000W on microspheres, concentrating to remove a solvent, and naturally drying to obtain plant growth regulator-loaded microspheres;
s6, preparation of the slow-release plant growth regulator: adding 35 parts by weight of the plant growth regulator-loaded microspheres prepared in the step S5 into 200 parts by weight of water, performing ultrasonic dispersion for 1 hour at 1000W, and adding 3.5 parts by weight of Co 2+ The slow-release plant growth regulator is prepared by stirring 4wt% of Tris-HCl solution with the pH value of 6, 45 parts of dopamine hydrochloride and 1.5 parts of ethylene oxide-propylene oxide triblock copolymer PEO106-PPO70-PEO106 at the rotating speed of 300r/min, reacting for 4 hours at 45 ℃, filtering and washing with water.
Comparative example 4
In comparison with example 3, the plant growth promoting composition of step S5 was not added, and other conditions were not changed.
The method comprises the following specific steps:
s1, porous hollow SiO 2 Preparing microspheres: dissolving 1.5 parts by weight of polyoxyethylene sorbitan fatty acid ester and 3 parts by weight of sodium octadecylbenzene sulfonate in 200 parts by weight of water, and dropwise adding 85 parts by weight of N-beta (beta)Stirring aminoethyl) -gamma-aminopropyltrimethoxysilane at the rotating speed of 1000r/min, reacting at 50 ℃ for 6h, centrifuging at 3000r/min for 15min, washing with deionized water, and spray-drying to obtain porous hollow SiO 2 Microspheres;
s2, preparation of nutrient substances: uniformly mixing 4 parts by weight of ammonium sulfate nitrate, 3 parts by weight of enriched calcium superphosphate and 0.7 part by weight of potassium sulfate to obtain a nutrient substance;
s3, preparing a duckweed diacolation extract: cleaning duckweed, drying at 70 ℃ for 2h, crushing to obtain duckweed powder, percolating and extracting for 2h by using 60wt% of ethanol solution, wherein the solid-to-liquid ratio of the duckweed powder to the 60wt% of ethanol solution is 1:7g/mL, merging percolate after complete percolation, recovering ethanol under reduced pressure, drying volatile solvent of a concentrate to obtain a duckweed percolation extract, and reserving the extracted duckweed powder for later use;
s4, preparing a duckweed fermentation extract: adding the extracted duckweed powder obtained in the step S3 into water, wherein the solid-to-liquid ratio of the extracted duckweed powder to the water is 1:4g/mL, inoculating activated bacillus subtilis liquid, activated acetic acid bacteria liquid and activated lactobacillus plantarum bacteria liquid, and the bacteria content of each bacteria liquid is 10 9 cfu/mL, inoculum size of 2%, 3%, 2.5%, 5% CO at 37 deg.C under 60% humidity 2 Fermenting for 56h under the volume content, filtering, and freeze-drying to obtain herba Spirodelae fermented extract;
s5, preparing the plant growth regulator loaded microspheres: dispersing 15 parts by weight of the nutrient substance obtained in step S2, 8 parts by weight of the duckweed diacolation extract obtained in step S3, and 8.5 parts by weight of the duckweed fermentation extract obtained in step S4 in 100 parts by weight of water, and adding 100 parts by weight of the porous hollow SiO solid obtained in step S1 2 Carrying out ultrasonic dispersion for 1h at 1000W on microspheres, concentrating to remove a solvent, and naturally drying to obtain plant growth regulator-loaded microspheres;
s6, preparation of a slow-release plant growth regulator: adding 35 parts by weight of the plant growth regulator-loaded microspheres prepared in the step S5 into 200 parts by weight of water, performing ultrasonic dispersion for 1 hour at 1000W, and adding 3.5 parts by weight of Co 2+ Tris-HCl solution with pH value of 6 and content of 4wt%, 45 weight parts of dopamine hydrochloride and 1.5 weight parts of ethylene oxide-propylene oxide triblock copolymer PEO106-PPO70-PEO106, stirring at the rotating speed of 300r/min, and carrying out reverse stirring at the temperature of 45 DEG CFiltering and washing for 4 hours to obtain the slow-release plant growth regulator.
Comparative example 5
Step S7 was not performed, and other conditions were not changed, as compared with example 3.
The method comprises the following specific steps:
s1, porous hollow SiO 2 Preparing microspheres: dissolving 1.5 parts by weight of polyoxyethylene sorbitan fatty acid ester and 3 parts by weight of sodium octadecylbenzene sulfonate in 200 parts by weight of water, dropwise adding 85 parts by weight of N-beta (aminoethyl) -gamma-aminopropyltrimethoxysilane, stirring at the rotating speed of 1000r/min, reacting at 50 ℃ for 6 hours, centrifuging at 3000r/min for 15min, washing with deionized water, and spray-drying to obtain porous hollow SiO 2 Microspheres;
s2, preparation of nutrient substances: uniformly mixing 4 parts by weight of ammonium sulfate nitrate, 3 parts by weight of enriched calcium superphosphate and 0.7 part by weight of potassium sulfate to obtain a nutrient substance;
s3, preparing a duckweed percolation extract: cleaning duckweed, drying at 70 ℃ for 2h, crushing to obtain duckweed powder, percolating and extracting for 2h by using 60wt% of ethanol solution, wherein the solid-to-liquid ratio of the duckweed powder to the 60wt% of ethanol solution is 1:7g/mL, merging percolates after complete percolation, recovering ethanol under reduced pressure, drying and volatilizing a solvent in a concentrate to obtain a duckweed percolation extract, and reserving the extracted duckweed powder;
s4, preparing a duckweed fermentation extract: adding the extracted duckweed powder obtained in the step S3 into water, wherein the solid-to-liquid ratio of the extracted duckweed powder to the water is 1:4g/mL, inoculating activated bacillus subtilis liquid, activated acetic acid bacteria liquid and activated lactobacillus plantarum bacteria liquid, and the bacteria content of each bacteria liquid is 10 9 cfu/mL, inoculum size of 2%, 3%, 2.5%, 5% CO at 37 deg.C under 60% humidity 2 Fermenting for 56h under the volume content, filtering, and freeze-drying to obtain a duckweed fermented extract;
s5, preparing a plant growth promoting composition: uniformly mixing 8.5 parts by weight of indolebutyric acid, 3.5 parts by weight of brassinosteroid and 1.5 parts by weight of jasmonic acid to obtain a plant growth promoting composition;
s6, preparation of the plant growth regulator loaded microspheres: mixing 15 weight parts of the nutrient substance prepared in the step S2 and 8 weight parts ofThe percolating extract of duckweed obtained in step S3, 8.5 parts by weight of the fermented extract of duckweed obtained in step S4, and 0.2 part by weight of the plant growth promoting composition obtained in step S5 were dispersed in 100 parts by weight of water, and 100 parts by weight of the porous hollow SiO solid obtained in step S1 was added 2 And (3) carrying out ultrasonic dispersion for 1h at 1000W on the microspheres, concentrating to remove the solvent, and naturally drying to obtain the plant growth regulator loaded microspheres.
Test example 1 sustained Release test
10mg of the slow-release plant growth regulator or the plant growth regulator-loaded microspheres prepared in examples 1-3 and comparative example 5 were added to a centrifuge tube, PBS buffer with pH 6.5 was added, the solution was subjected to ultracentrifugation (20000r/min, 20min) at regular intervals at a constant temperature of 25 ℃ and at a speed of 200r/min for 0.5, 1, 2, 6, 12d, and the release rate was calculated according to the following formula:
release rate (%) = (W) t -W 0 ）/W 0 ×100%
In the formula, W t Is the initial weight of the microspheres; w 0 The microspheres are weighed after a certain time in an acidic PBS buffer.
The results are shown in Table 1.
TABLE 1
Figure 867443DEST_PATH_IMAGE001
As can be seen from the above table, the slow-release plant growth regulator prepared in the embodiments 1 to 3 of the present invention has a good slow-release effect.
Comparative example 5 compared with example 3, the slow release effect is significantly reduced without coating modification of polydopamine in step S7, and the present invention immobilizes the nutrient, the duckweed percolation extract, the duckweed fermentation extract, and the plant growth promoting composition on the porous hollow SiO 2 In the microspheres, the microspheres contain rich plant growth promoting substances, the plant growth regulator can be continuously released through pores on the surfaces of the microspheres, and the surfaces of the prepared plant growth regulator-loaded microspheres are coated with a polydopamine layer, so that the release rate of the plant growth regulator can be further slowed down, and meanwhile, the polydopamine layerThe amine is rich in hydroxyl, sulfydryl, amino, carboxyl and other groups, and can promote the prepared slow-release plant growth regulator to be fixed on the surface of a relevant part of a plant, so that the plant growth regulator can be released fixedly for a long time, the product disclosed by the invention does not need to be sprayed for multiple times, the plant growth regulator is released for a long time, and the action aging of the plant growth regulator is prolonged.
Test example 2 growth promoting action on Broussonetia papyrifera
Taking the tissue culture seedlings of the Keji 101 as test objects, selecting the tissue culture seedlings with consistent growth vigor, and planting the tissue culture seedlings in the middle ten days of 4 months with the density of 400 plants/mu. 1 mu was divided equally into 14 groups, which were examples 1-8 groups and comparative examples 1-5 groups, control group, respectively. Spraying the solution prepared from the products prepared in examples 1-8 and comparative examples 1-5 (every 1g of product dissolved in 100g of water) at 100 g/plant after 20 days of planting, only 1 time throughout the growth period, and the control group with the same amount of clear water.
Measuring overground growth indexes in 7-month last ten days: plant height, stem thickness (expressed as the average of the stem thickness at the 3 rd node above the base), main stem node number (all nodes of the main stem are measured), and branch number (the branch number at the base of the main stem is measured at 10cm or less).
The results are shown in Table 2.
TABLE 2
Figure 88340DEST_PATH_IMAGE002
As can be seen from the above table, the slow-release plant growth regulator prepared in the embodiments 1 to 3 of the present invention has an obvious effect of promoting the growth, development and branching of paper mulberry.
Collecting 3 seedlings in each group, deactivating enzyme at 105 deg.C for 15min, drying at 70 deg.C to constant weight, respectively crushing 3 seedlings after drying, and mixing well for determining nutritional components. The crude protein is measured by adopting a Kjeldahl method, and the contents of neutral detergent fiber, acidic detergent fiber and lignin are measured by adopting a polyester mesh bag method (reference documents: Zhang Chen et al. research on the content of crude fiber in the feed by the polyester mesh bag method [ J ]. animal nutrition report, 2019,31(6): 2875-2881).
The results are shown in Table 3.
TABLE 3
Figure 441961DEST_PATH_IMAGE003
As can be seen from the above table, the slow-release plant growth regulator prepared in the embodiments 1 to 3 of the present invention has a significantly improved effect on the enrichment of broussonetia papyrifera nutrients.
Compared with example 3, the nutrition substance of S2 is not added, the promotion effect on the growth and development of the paper mulberry is reduced, and the nutrition enrichment effect on the paper mulberry is reduced in comparative example 1. The preparation of the slow-release plant growth regulator is added with nutrient substances, including nitrogen fertilizer, phosphate fertilizer and potassium fertilizer, applied to relevant parts of plants, beneficial to the absorption of the plants through surface capillary channels and providing nutrients required for promoting the growth of the plants, thereby ensuring the nutrient supply while the plants grow rapidly and ensuring the healthy growth of the plants.
Comparative example 2 compared to example 3, the addition of the duckweed diacolation extract of step S3 was less effective in promoting the growth and development of paper mulberry. In examples 6, 7 and 8, compared with example 3, no bacillus subtilis solution, acetic acid bacteria solution or lactobacillus plantarum solution was added in step S4; comparative example 3 compared to example 3, the duckweed fermented extract of step S4, which is not added, has a reduced promoting effect on the growth and development of broussonetia papyrifera and a reduced nutrition enrichment effect on broussonetia papyrifera. The duckweed is rich in polysaccharide, flavonoid, orientin, vitexin, linolenic acid, luteolin and the like, most nutrient substances are dissolved out by percolation and fermentation extraction to obtain rich nutrient substances, and the duckweed is one of the plants with the fastest growth speed in the world, it contains rich substances for promoting plant growth, and these substances and nutrient substances act synergistically, not only can supplement nutrients in plants in time, but also can improve plant productivity and resistance to external adverse environment by regulating plant physiological state and improving physiological function, enhance plant osmotic regulation capability, promote chloroplast formation, improve plant water and sunlight utilization efficiency and cell membrane stability, thereby promoting the nutrient enrichment of plants, enhancing the early warning and reaction capability of the plants to adverse environment, and further improving the effects of drought resistance, salt resistance, cold resistance and pest and disease damage inhibition of the plants. The three fermentation strains produce different nutrients by fermentation, have different promoting effects on the growth of plants, and have a synergistic effect.
Examples 4 and 5 compared with example 3, no indolebutyric acid or brassinosteroid was added in step S5, which resulted in a decrease in the growth and development promoting effect on broussonetia papyrifera; comparative example 4 compared to example 3, the plant growth promoting composition of step S5, which was not added, had a decreased promoting effect on the growth and development of broussonetia papyrifera and a decreased nutrient enrichment effect on broussonetia papyrifera. The added plant growth promoting composition comprises indolebutyric acid, brassinosteroid and jasmonic acid, and has good effect of promoting plant growth, wherein the indolebutyric acid has good effect of inducing formation of root protomer, promoting cell differentiation and division, facilitating generation of new roots and differentiation of vascular bundle systems, promoting formation of adventitious roots of cuttings, improving photosynthetic efficiency, promoting accumulation of photosynthetic products and promoting cell division; brassinosteroids high-activity substances extracted from rape pollen and capable of promoting plant stem elongation and cell division transmit signals through cell membrane surface receptors, and can strongly induce growth and differentiation; jasmonic acid also has a certain effect on promoting the formation of plant epidermal hair, inhibits the growth and development of the plant epidermal hair by causing the change of plant gene expression, resists the adverse stress of the external environment, increases the content of secondary metabolites and improves the stress resistance of the plant, so the plant growth regulator composition added in the invention can promote the growth of the plant, improve the stress resistance of the plant, obviously improve the growth and development level of the plant and has the synergistic effect. The three regulators have synergistic effect.
Comparative example 5 compared to example 3, step S7 was not performed, and the accelerating effect on the growth and development of broussonetia papyrifera and the nutrient enrichment effect on broussonetia papyrifera were decreased. This is because, in comparative example 5, no polydopamine coating modification is adopted, the slow release effect of the prepared microspheres on the plant growth regulator is reduced, the microspheres have an obvious promoting effect on the plant at the beginning of the period, and the promoting effect on the plant is obviously reduced after the microspheres are rapidly released along with the passage of time, so that the growth rate of the whole plant is reduced, and the enrichment effect on nutrients is reduced.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.

Claims (10)

1. A preparation method of a slow-release plant growth regulator is characterized in that aminosilane is added into water with a pore-forming agent and a surfactant, and the mixture is stirred to react to obtain porous hollow SiO 2 Dispersing the microspheres in water, adding nutrient substances, a duckweed percolation extract, a duckweed fermentation extract and a plant growth promoting composition, concentrating, naturally drying to obtain plant growth regulator loaded microspheres, and further coating the surfaces of the plant growth regulator loaded microspheres with a polydopamine layer to obtain a slow-release plant growth regulator;
the nutrient substances consist of nitrogenous fertilizer, phosphate fertilizer and potash fertilizer;
the plant growth promoting composition comprises indolebutyric acid, brassinosteroid and jasmonic acid.
2. The method of claim 1, comprising the steps of:
s1, porous hollow SiO 2 Preparing microspheres: dissolving a first pore-forming agent and a surfactant in water, dropwise adding aminosilane, stirring for reaction, centrifugally washing, and spray-drying to obtain porous hollow SiO 2 Microspheres;
s2, preparation of nutrient substances: uniformly mixing a nitrogenous fertilizer, a phosphate fertilizer and a potash fertilizer to obtain a nutrient substance;
s3, preparing a duckweed diacolation extract: cleaning duckweed, drying, pulverizing to obtain duckweed powder, percolating with ethanol solution, mixing percolates after complete percolation, recovering ethanol under reduced pressure, drying the concentrate to volatilize solvent to obtain duckweed percolate extract, and reserving the extracted duckweed powder for later use;
s4, preparing a duckweed fermentation extract: adding the extracted duckweed powder obtained in the step S3 into water, inoculating activated zymocyte liquid, fermenting, filtering, and freeze-drying to obtain a duckweed fermentation extract;
s5, preparing a plant growth promoting composition: uniformly mixing indolebutyric acid, brassinosteroid and jasmonic acid to obtain a plant growth promoting composition;
s6, preparation of the plant growth regulator loaded microspheres: dispersing the nutrient substance obtained in step S2, the percolating extract of duckweed obtained in step S3, the fermented extract of duckweed obtained in step S4, and the plant growth promoting composition obtained in step S5 in water, and adding the porous hollow SiO obtained in step S1 2 Dispersing the microspheres uniformly, concentrating to remove the solvent, and naturally drying to obtain plant growth regulator-loaded microspheres;
s7, preparation of the slow-release plant growth regulator: and (4) uniformly dispersing the plant growth regulator-loaded microspheres prepared in the step (S6) in water, adding a catalyst solution, dopamine hydrochloride and a second pore-forming agent, stirring for reaction, filtering, and washing with water to obtain the slow-release plant growth regulator.
3. The method according to claim 2, wherein the aminosilane in step S1 is at least one selected from the group consisting of 3-aminopropyltriethoxysilane, γ -aminopropyltrimethoxysilane, N- β (aminoethyl) - γ -aminopropylmethyldimethoxysilane, N- β (aminoethyl) - γ -aminopropyltriethoxysilane, N- β (aminoethyl) - γ -aminopropylmethyldiethoxysilane, and diethylenetriaminopropyltrimethoxysilane; the first pore-forming agent is polyoxyethylene sorbitan fatty acid ester or polyethylene glycol octyl phenyl ether; the surfactant is selected from at least one of sodium dodecyl benzene sulfonate, sodium dodecyl sulfate, sodium hexadecyl benzene sulfonate, sodium hexadecyl sulfate, sodium octadecyl sulfonate and sodium octadecyl benzene sulfonate; the mass ratio of the first pore-forming agent to the surfactant to the aminosilane is 1-2:2-4: 70-100.
4. The method according to claim 2, wherein the nitrogen fertilizer in step S2 is selected from at least one of urea, ammonium nitrate, ammonium sulfate, ammonium chloride, ammonium bicarbonate, ammonia water, sodium nitrate, calcium ammonium nitrate and ammonium sulfate nitrate; the phosphate fertilizer is selected from at least one of sodium phosphate, calcium superphosphate, triple superphosphate, enriched superphosphate and ammonium phosphate; the potash fertilizer is at least one of potassium chloride, potassium sulfate and potassium nitrate; the mass ratio of the nitrogenous fertilizer to the phosphate fertilizer to the potash fertilizer is 3-5:2-4: 0.5-1; in the step S3, the solid-to-liquid ratio of the duckweed powder to the ethanol solution is 1:5-10 g/mL; the ethanol content in the ethanol solution is 50-70 wt%; the percolation extraction time is 1-3 h.
5. The preparation method according to claim 2, wherein the solid-to-liquid ratio of the extracted duckweed powder to water in step S4 is 1:3-5 g/mL; the zymocyte is bacillus subtilis, acetic acid bacteria and lactobacillus plantarum; the bacteria content of the activated zymophyte liquid is 10 8 -10 9 cfu/mL; the inoculation amounts of the bacillus subtilis, the acetic acid bacteria and the lactobacillus plantarum are respectively 1-3%, 2-4% and 2-3%; the fermentation conditions are 35-40 deg.C, 50-70% humidity, and 3-7% CO 2 Fermenting for 48-72h under the volume content.
6. The preparation method according to claim 2, wherein the mass ratio of the indole butyric acid, the brassinosteroid and the jasmonic acid in the step S5 is 7-10:2-5: 1-2; in the step S6, the mass ratio of the nutrient substances, the duckweed percolation extract, the duckweed fermentation extract and the plant growth promoting composition is 10-20:5-12:7-10: 0.1-0.3.
7. The preparation method of claim 2, wherein the mass ratio of the plant growth regulator-loaded microspheres, the catalyst solution, the dopamine hydrochloride and the second pore-forming agent in step S7 is 20-50:2-5:30-60: 1-2; the catalyst solution is Co 2+ Tris-HCl solution with pH value of 5.5-6.5 and content of 3-5wt%The second pore-foaming agent is selected from at least one of cetyl trimethyl ammonium bromide, ethylene oxide-propylene oxide triblock copolymer PEO20-PPO70-PEO20 and PEO106-PPO70-PEO 106.
8. The preparation method according to claim 2, characterized by comprising the following steps:
s1, porous hollow SiO 2 Preparing microspheres: dissolving 1-2 parts by weight of first pore-foaming agent and 2-4 parts by weight of surfactant in 200 parts by weight of water, dropwise adding 70-100 parts by weight of aminosilane, stirring for reaction, centrifugally washing, and spray drying to obtain porous hollow SiO 2 Microspheres;
s2, preparation of nutrient substances: uniformly mixing 3-5 parts by weight of nitrogenous fertilizer, 2-4 parts by weight of phosphate fertilizer and 0.5-1 part by weight of potash fertilizer to obtain nutrient substances;
s3, preparing a duckweed diacolation extract: cleaning duckweed, drying, crushing to obtain duckweed powder, percolating and extracting for 1-3h by using 50-70wt% of ethanol solution, wherein the solid-to-liquid ratio of the duckweed powder to the 50-70wt% of ethanol solution is 1:5-10g/mL, merging percolate after complete percolation, recovering ethanol under reduced pressure, drying and volatilizing a solvent of a concentrate to obtain a duckweed percolation extract, and reserving the extracted duckweed powder for later use;
s4, preparing a duckweed fermentation extract: adding the extracted duckweed powder obtained in the step S3 into water, wherein the solid-to-liquid ratio of the extracted duckweed powder to the water is 1:3-5g/mL, and the inoculation bacteria content is 10 8 -10 9 The cfu/mL activated zymocyte liquid is respectively bacillus subtilis liquid, acetic acid bacteria liquid and lactobacillus plantarum liquid, the inoculation amount is respectively 1-3%, 2-4% and 2-3%, and 3-7% CO is obtained at 35-40 ℃ and 50-70% humidity 2 Fermenting for 48-72h under the volume content, filtering, and freeze-drying to obtain herba Spirodelae fermented extract;
s5, preparing a plant growth promoting composition: uniformly mixing 7-10 parts by weight of indolebutyric acid, 2-5 parts by weight of brassinosteroids and 1-2 parts by weight of jasmonic acid to obtain a plant growth promoting composition;
s6, preparation of the plant growth regulator loaded microspheres: 10 to 20 weight portions of the nutrient substance prepared in the step S2 and 5 to 12 weight portionsDispersing the diacolation extract of duckweed obtained in step S3, the fermentation extract of duckweed obtained in step S4, and the plant growth promoting composition obtained in step S5 in water in an amount of 7-10 parts by weight, and adding the porous hollow SiO obtained in step S1 in an amount of 100 parts by weight to 100 parts by weight 2 Dispersing the microspheres uniformly, concentrating to remove the solvent, and naturally drying to obtain the plant growth regulator loaded microspheres;
s7, preparation of the slow-release plant growth regulator: uniformly dispersing 20-50 parts by weight of the plant growth regulator-loaded microspheres prepared in the step S6 in 200 parts by weight of water, and adding 2-5 parts by weight of Co 2+ The slow-release plant growth regulator is prepared by stirring and reacting 3-5wt% of Tris-HCl solution with the pH value of 5.5-6.5, 30-60 parts of dopamine hydrochloride and 1-2 parts of second pore-forming agent by weight, filtering and washing with water.
9. A slow-release plant growth regulator prepared by the preparation method as claimed in any one of claims 1 to 8.
10. Use of the slow-release plant growth regulator according to claim 9 for promoting plant growth for a long period of time.
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