CN115023506A - 测定病毒滴度的方法 - Google Patents

测定病毒滴度的方法 Download PDF

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Publication number
CN115023506A
CN115023506A CN202180011209.6A CN202180011209A CN115023506A CN 115023506 A CN115023506 A CN 115023506A CN 202180011209 A CN202180011209 A CN 202180011209A CN 115023506 A CN115023506 A CN 115023506A
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Prior art keywords
viral titer
cell
cells
virus
titer
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Pending
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CN202180011209.6A
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English (en)
Chinese (zh)
Inventor
Y·申
A·赛斯
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Lonza Walkersville Inc
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Lonza Walkersville Inc
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Publication of CN115023506A publication Critical patent/CN115023506A/zh
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    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14141—Use of virus, viral particle or viral elements as a vector
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    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
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    • C12Q2523/00—Reactions characterised by treatment of reaction samples
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    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/149—Particles, e.g. beads
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    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/159—Microreactors, e.g. emulsion PCR or sequencing, droplet PCR, microcapsules, i.e. non-liquid containers with a range of different permeability's for different reaction components
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    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/136—Screening for pharmacological compounds

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  • Virology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
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CN202180011209.6A 2020-01-28 2021-01-26 测定病毒滴度的方法 Pending CN115023506A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202062966639P 2020-01-28 2020-01-28
US62/966,639 2020-01-28
PCT/US2021/014987 WO2021154672A1 (en) 2020-01-28 2021-01-26 Methods of determining viral titer

Publications (1)

Publication Number Publication Date
CN115023506A true CN115023506A (zh) 2022-09-06

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CN202180011209.6A Pending CN115023506A (zh) 2020-01-28 2021-01-26 测定病毒滴度的方法

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US (1) US20230038151A1 (he)
EP (1) EP4087949A1 (he)
JP (1) JP2023511108A (he)
KR (1) KR20220133908A (he)
CN (1) CN115023506A (he)
CA (1) CA3163787A1 (he)
IL (1) IL295154A (he)
WO (1) WO2021154672A1 (he)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2024014439A1 (ja) * 2022-07-11 2024-01-18 アステラス製薬株式会社 生体内の目的ｄｎａ定量法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG11202004406VA (en) * 2017-11-08 2020-06-29 Avexis Inc Means and method for preparing viral vectors and uses of same
CA3088690A1 (en) * 2018-02-02 2019-08-08 University Of Massachusetts Campaign-ready series of recombinant adeno-associated virus (raav) complementing plasmids
US20210010028A1 (en) * 2018-03-06 2021-01-14 Voyager Therapeutics, Inc. Insect cell manufactured partial self-complementary aav genomes

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Publication number Publication date
CA3163787A1 (en) 2021-08-05
KR20220133908A (ko) 2022-10-05
US20230038151A1 (en) 2023-02-09
EP4087949A1 (en) 2022-11-16
WO2021154672A1 (en) 2021-08-05
IL295154A (he) 2022-09-01
JP2023511108A (ja) 2023-03-16

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