CN115006476B - Preparation method of eyebright rolling agent and eyebright rolling agent - Google Patents
Preparation method of eyebright rolling agent and eyebright rolling agent Download PDFInfo
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Abstract
The invention discloses a preparation method of an eyebright roll agent and the eyebright roll agent. The product prepared by the method of the invention creates the product dosage form, has the functions of detumescence, activating blood circulation to dissipate blood stasis, relieving eye fatigue and treating acute conjunctivitis, avoids the factors influenced by seasons, can meet the requirements of using at different periods, increasing the storage time, can achieve the purpose of safe use, fully embodies the superiority of the invention, and is suitable for popularization and application.
Description
Technical Field
The invention relates to the technical field of plant extraction and application, and particularly relates to a preparation method of an eyebright rolling agent and the eyebright rolling agent.
Background
The Ezikia indica belongs to Eziaceae, and is a perennial aquatic herb commonly known as Duck grass, water chopping board or water floating. Ming Dynasty blue Mao recorded in Dian nan materia Medica, tooth grass, also named tooth picking grass. In the raw field, it is bitter and astringent in taste and cold in nature. Stop red and white dysentery, large intestine bleeding, woman red metrorrhagia, metrostaxis and aversion to blood. It is recorded in Chinese medicine dictionary that Ezicai has the functions of clearing away heat, promoting diuresis, arresting bleeding, eliminating swelling and expelling ascaris, and has the pharmacological actions of promoting urination, arresting bleeding, killing parasite, etc. and is used in treating piles, ascaris, edema and other diseases. According to records in Guiyang folk herb, ezicai can be used for treating fire eyes by picking up a plurality of fresh Ezicai leaves, sticking the Ezicai leaves on eyelid, and changing the Ezicai leaves after the Ezicai leaves are dry. According to records in Shanxi Chinese herbal medicine, the eyedrops can be used for treating sores and furuncles: the method is that a proper amount of fresh leaves of the eyedrop herb are picked, pounded and externally applied, and according to the record of Shaanxi Chinese herbal medicine, the eyedrop herb can also be used for treating acute conjunctivitis, toothache, jaundice, sore, furuncle, carbuncle and swelling. The eyedrops have the functions of clearing heat, improving eyesight, inducing diuresis and reducing edema, and are used for treating various bleeding, fire eyes and other symptoms, and only crude drugs are used for external use and decoction for oral administration. According to previous researches, the gynura divaricata contains flavonoids, volatile oils, amino acids, saccharides, tannins, phenols and other components, wherein the flavonoids are main active substances of the gynura divaricata.
The ball bottle is also called a ball bottle, is a common packaging bottle and is widely used by people. Is usually used as cosmetic eye cream, lipstick and children product. The ball bottle is usually not large in capacity, and balls are mounted at the bottle head, so that people can uniformly paint the bottle, liquid is prevented from being omitted, and the bottle also has a massage effect.
Through retrieval, no relevant patent literature information about the clitocybe japonica ballpoint agent is found, and the clitocybe japonica serving as a medicinal material with remarkable drug effect and wide application is simple in the current application mode, mainly used by directly picking fresh leaves of the clitocybe japonica ballpoint agent, influenced by the growing season due to the fact that the clitocybe japonica ballpoint agent is picked during use and cannot be used at different periods. Therefore, the eyedrops can be made into finished ball agent products in order to avoid the influence of seasons, and the eyedrops are not limited by time and are convenient to apply.
Disclosure of Invention
The invention aims to solve the technical problems in the background art and provides a preparation method of an eyedrop, the ball agent prepared by the method is more convenient in the using process and can prolong the storage time of the eyedrop, the prepared ball agent can fully utilize the efficacy of medicines and plays roles of promoting absorption and relieving fatigue through massage, and particularly the preparation method of the eyedrop ball agent and the eyedrop ball agent.
In order to solve the technical problems, the technical scheme adopted by the invention is as follows: a preparation method of an eyebright roll agent comprises the following steps of taking eyebright as a raw material, decocting, extracting, filtering, refining, separating, concentrating, adding auxiliary materials to prepare an eyebright liquid, and finally filling the eyebright liquid into a roll bottle to obtain the eyebright roll agent, wherein the preparation method comprises the following steps:
the method comprises the following steps: preparation of refined liquid of potamogeton muelleri
(1) Preparing a primary liquid: taking a proper amount of gynura bicolor, soaking the gynura bicolor in clear water for 20 minutes, putting the gynura bicolor into a marmite or a stainless steel pot, adding clear water for decocting twice, wherein the amount of the clear water added for the first time is 8-12 times of the weight of the gynura bicolor, the amount of the clear water added for the second time is 5-7 times of the weight of the gynura bicolor, the first decocting time is 35-45 minutes, the second decocting time is 15-25 minutes, removing dregs of a decoction through twice decocting, and combining the twice decocted liquid medicines to obtain a primary liquid;
(2) And (3) filtering: filtering the primary liquid obtained after extraction by adopting a 120-200 mesh filter screen to obtain filtrate;
(3) Refining, separating and concentrating: concentrating the filtrate to relative density of 1.0-1.1 to obtain concentrated solution, adding absolute ethanol to make the ethanol content of the Chinese medicinal liquid in the concentrated solution reach 70%, standing for 10-20 hours, collecting supernatant, and recovering ethanol to obtain refined stock solution of herba EZICAI;
(4) Preparing refined liquid: adding sodium benzoate into the refined stock solution, wherein the addition amount of the sodium benzoate is 0.1-0.5% of the weight of the refined stock solution, adding the sodium benzoate, stirring for dissolving, filtering with a 200-mesh filter screen after stirring uniformly, and collecting the filtrate to obtain the refined liquid of the gynura bicolor for later use;
step two: treatment of ball bottles
(1) Initial washing: selecting a ball bottle with the specification of 10 ml, firstly adding a proper amount of drinking water to soak for 1 hour, then ultrasonically cleaning for 6 minutes by adopting an ultrasonic machine with the power of 200W, and taking out for later use after ultrasonic cleaning;
(2) Fine washing: cleaning the primarily cleaned ball bottle with purified water for 3 times;
(3) And (3) sterilization: putting the ball bottle after the fine cleaning into a stainless steel pot filled with gauze, boiling for 60 minutes, taking out, and drying in an oven at the temperature of 80 ℃ for 1 hour;
step three: filling and sterilizing
(1) Filling: filling the prepared refined liquid of the gynura divaricata into ball bottles after sterilization treatment, wherein the filling amount of each bottle is not less than 8.5ml;
(2) And (3) sterilization: placing the ball agent filled with the fine liquid of the Ezikia indica in an HJ-1405 ultraviolet sterilizer, adjusting the sterilization wavelength to 254nm, sterilizing for 60 minutes, sterilizing, and cooling to normal temperature to obtain the product of the Ezikia indica ball agent.
Further, in the preparation method of the eyedrop ball agent, in the process of preparing the primary liquid, the raw materials are soaked in clean water for 30 minutes, wherein the amount of the clean water added for the first time is 10 times of the weight of the eyedrop, the amount of the clean water added for the second time is 6 times of the weight of the eyedrop, the first decocting time is 40 minutes, and the second decocting time is 20 minutes.
Further, the preparation method of the eyebright ball agent provided by the invention comprises the steps of filtering by using a 180-200 mesh filter screen in the filtering process, and collecting filtrate after filtering.
Furthermore, the preparation method of the eyedrop ball agent provided by the invention is characterized in that in the process of preparing the refined liquid, the addition amount of the sodium benzoate is 0.3% of the weight of the refined stock solution, and the sodium benzoate is sodium benzoate solid.
The invention also discloses the eyebright rolling agent prepared by the method.
Compared with the prior art, the preparation method of the eyebright ball agent and the eyebright ball agent have the beneficial effects that: the flavonoids have the effects of resisting oxidation, cancer, angiogenesis, inflammation, allergy and virus, and are prepared by decocting, extracting, filtering, refining, separating and concentrating the raw materials of the gynura divaricata, adding auxiliary materials to prepare the refined gynura divaricata liquid, and finally filling the refined gynura divaricata liquid in a ball bottle. The product of the eyedrop ball agent prepared by the method of the invention creates the product dosage form, has the functions of detumescence, activating blood circulation to dissipate blood stasis, relieving eye fatigue and treating acute conjunctivitis, not only avoids the factors influenced by seasons, but also can meet the requirements of using at different periods, increasing the storage time, achieving the purpose of safe use, fully embodying the superiority of the invention and being suitable for popularization and application.
Detailed Description
In order to more fully explain the practice of the invention, the invention is further described with reference to the following specific examples. The examples are given solely for the purpose of illustration and are not intended to limit the scope of the invention.
Example 1:
a preparation method of an eyebright ball agent takes eyebright as a raw material, and comprises the following steps of decocting and extracting, filtering, refining and separating, concentrating, adding auxiliary materials to prepare an eyebright liquid, and finally filling the eyebright liquid in a ball bottle, wherein the preparation method comprises the following steps:
the method comprises the following steps: preparation of refined liquid of eyedrops
(1) Preparing a primary liquid: taking a proper amount of gynura bicolor, soaking the gynura bicolor in clear water for 20 minutes, putting the gynura bicolor into a marmite or a stainless steel pot, adding clear water, decocting twice, wherein the amount of the clear water added for the first time is 8 times of the weight of the gynura bicolor, the amount of the clear water added for the second time is 5 times of the weight of the gynura bicolor, the first decocting time is 35 minutes, the second decocting time is 15 minutes, removing dregs through twice decocting, and combining the decocted liquid medicines to obtain a primary liquid;
(2) And (3) filtering: filtering the primary liquid obtained after extraction by adopting a 120-mesh filter screen to obtain a filtrate;
(3) Refining, separating and concentrating: concentrating the filtrate to relative density of 1.0-1.05 to obtain concentrated solution, adding anhydrous ethanol to make the ethanol content of the Chinese medicinal liquid in the concentrated solution reach 70%, standing for 10 hours, collecting supernatant, and recovering ethanol to obtain refined stock solution of herba Equiseti Arvinsis;
(4) Preparing refined liquid: adding sodium benzoate solid into the refined stock solution, wherein the adding amount of the sodium benzoate is 0.1% of the weight of the refined stock solution, adding the sodium benzoate, stirring for dissolving, filtering by using a 200-mesh filter screen after uniformly stirring, and collecting the filtrate to obtain the fine liquid of the eyedrops for later use;
step two: treatment of ball bottles
(1) Initial washing: selecting a ball bottle with the specification of 10 ml, firstly adding a proper amount of drinking water to soak for 1 hour, then ultrasonically cleaning for 6 minutes by adopting an ultrasonic machine with the power of 200W, and taking out for later use after ultrasonic cleaning;
(2) Fine washing: cleaning the primarily cleaned ball bottle with purified water for 3 times;
(3) And (3) sterilization: putting the ball bottle after the fine cleaning into a stainless steel pot filled with gauze, boiling for 60 minutes, taking out, and drying in an oven at the temperature of 80 ℃ for 1 hour;
step three: filling and sterilizing
(1) Filling: filling the prepared refined liquid of the gynura divaricata into ball bottles after sterilization treatment, wherein the filling amount of each bottle is not less than 8.5ml;
(2) And (3) sterilization: placing the ball agent filled with herba EZICAI refined solution in HJ-1405 type ultraviolet sterilizer, adjusting sterilization wavelength to 254nm, sterilizing for 60 min, sterilizing, and cooling to room temperature to obtain the final product.
Example 2:
a preparation method of an eyebright roll agent comprises the following steps of taking eyebright as a raw material, decocting, extracting, filtering, refining, separating, concentrating, adding auxiliary materials to prepare an eyebright liquid, and finally filling the eyebright liquid into a roll bottle to obtain the eyebright roll agent, wherein the preparation method comprises the following steps:
the method comprises the following steps: preparation of refined liquid of potamogeton muelleri
(1) Preparing a primary liquid: taking a proper amount of gynura bicolor, soaking the gynura bicolor in clear water for 30 minutes, putting the gynura bicolor into a marmite or a stainless steel pot, adding clear water, decocting twice, wherein the amount of the clear water added for the first time is 10 times of the weight of the gynura bicolor, the amount of the clear water added for the second time is 6 times of the weight of the gynura bicolor, the first decocting time is 40 minutes, the second decocting time is 20 minutes, removing dregs through twice decocting, and combining the decocted liquid medicines to obtain a primary liquid;
(2) And (3) filtering: filtering the primary liquid obtained after extraction by using a 200-mesh filter screen to obtain a filtrate;
(3) Refining, separating and concentrating: concentrating the filtrate to relative density of 1.04-1.08 to obtain concentrated solution, adding anhydrous ethanol solution to make the ethanol content of the Chinese medicinal liquid in the concentrated solution reach 70%, standing for 15 hours, collecting supernatant, and recovering ethanol to obtain refined stock solution of herba EZICAI;
(4) Preparing a refined solution: adding sodium benzoate solid into the refined stock solution, wherein the adding amount of the sodium benzoate is 0.35% of the weight of the refined stock solution, adding the sodium benzoate, stirring for dissolving, filtering by using a 200-mesh filter screen after uniformly stirring, and collecting the filtrate to obtain the fine liquid of the eyedrops for later use;
step two: treatment of ball bottles
(1) Initial washing: selecting a ball bottle with the specification of 10 ml, firstly adding a proper amount of drinking water to soak for 1 hour, then ultrasonically cleaning for 6 minutes by adopting an ultrasonic machine with the power of 200W, and taking out for later use after ultrasonic cleaning;
(2) Fine washing: cleaning the primarily cleaned ball bottle with purified water for 3 times;
(3) And (3) sterilization: putting the ball bottle after the fine cleaning into a stainless steel pot filled with gauze, boiling for 60 minutes, taking out, and drying in an oven at the temperature of 80 ℃ for 1 hour;
step three: filling and sterilizing
(1) Filling: filling the prepared refined liquid of the gynura divaricata into ball bottles after sterilization treatment, wherein the filling amount of each bottle is not less than 8.5ml;
(2) And (3) sterilization: placing the ball agent filled with herba EZICAI refined solution in HJ-1405 type ultraviolet sterilizer, adjusting sterilization wavelength to 254nm, sterilizing for 60 min, sterilizing, and cooling to room temperature to obtain the final product.
Example 3:
a preparation method of an eyebright ball agent takes eyebright as a raw material, and comprises the following steps of decocting and extracting, filtering, refining and separating, concentrating, adding auxiliary materials to prepare an eyebright liquid, and finally filling the eyebright liquid in a ball bottle, wherein the preparation method comprises the following steps:
the method comprises the following steps: preparation of refined liquid of eyedrops
(1) Preparing a primary liquid: taking a proper amount of gynura bicolor, soaking the gynura bicolor in clear water for 40 minutes, putting the gynura bicolor into a marmite or stainless steel pot, adding clear water, decocting twice, wherein the amount of the clear water added for the first time is 12 times of the weight of the gynura bicolor, the amount of the clear water added for the second time is 7 times of the weight of the gynura bicolor, the time for the first decoction is 45 minutes, the time for the second decoction is 25 minutes, removing dregs of a decoction through the two times of decoction, and combining the liquid medicines decocted for the two times to obtain a primary liquid;
(2) And (3) filtering: filtering the primary liquid obtained after extraction by adopting a 180-mesh filter screen to obtain a filtrate;
(3) Refining, separating and concentrating: concentrating the filtrate to relative density of 1.07-1.1 to obtain concentrated solution, adding absolute ethanol to make the ethanol content of the Chinese medicinal liquid in the concentrated solution reach 70%, standing for 20 hours, collecting supernatant, and recovering ethanol to obtain refined stock solution of herba EZICAI;
(4) Preparing a refined solution: adding sodium benzoate solid a into the refined stock solution, adding the sodium benzoate according to the proportion of 0.5 percent of the weight of the refined stock solution, stirring and dissolving the sodium benzoate, filtering the solution by using a 200-mesh filter screen after uniformly stirring, and collecting the filtrate to obtain the fine liquid of the eyedrops for later use;
step two: treatment of ball bottles
(1) Initial washing: selecting a ball bottle with the specification of 10 ml, firstly adding a proper amount of drinking water to soak for 1 hour, then ultrasonically cleaning for 6 minutes by adopting an ultrasonic machine with the power of 200W, and taking out for later use after ultrasonic cleaning;
(2) Fine washing: cleaning the primarily cleaned ball bottle with purified water for 3 times;
(3) And (3) sterilization: putting the ball bottle after the fine cleaning into a stainless steel pot filled with gauze, boiling for 60 minutes, taking out, and drying in an oven at the temperature of 80 ℃ for 1 hour;
step three: filling and sterilizing
(1) Filling: filling the prepared refined liquid of the gynura divaricata into ball bottles after sterilization treatment, wherein the filling amount of each bottle is not less than 8.5ml;
(2) And (3) sterilization: placing the ball agent filled with the fine liquid of the Ezikia indica in an HJ-1405 ultraviolet sterilizer, adjusting the sterilization wavelength to 254nm, sterilizing for 60 minutes, sterilizing, and cooling to normal temperature to obtain the product of the Ezikia indica ball agent.
Pharmacological experiments are carried out to show that the gynura divaricata ball agent prepared by the method has the effects of reducing swelling, promoting blood circulation and removing blood stasis. Pharmacological experiments were performed using the eyedish ball agent prepared as described in example 2, a coating treatment experiment was performed for acute soft tissue contusion of the foot of a white rat caused by an impactor, the coating was performed twice a day, and comparative experiments were performed by a blank control (without any agent), and the experimental results are shown in table 1.
TABLE 1 Effect on recovery time of acute soft tissue injury in white rat feet (t-test P < 0.05)
| Group of | Animal number (only) | Recovery time h (X + -SD) |
| Blank group | 10 | 235±7.8 |
| Test group | 10 | 112±3.5* |
As can be seen from Table 1, pharmacological experiments prove that the eyebright ball agent can obviously shorten the recovery time and has the functions of detumescence and stasis dissipation.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the present invention, and it will be understood by those skilled in the art that various changes and modifications may be made therein without departing from the spirit and scope of the invention.
Claims (4)
1. A preparation method of an eyebright ball agent is characterized in that eyebright is used as a raw material, and is prepared by decocting, extracting, filtering, refining, separating and concentrating the raw material, adding auxiliary materials to prepare an eyebright liquid, and finally filling the eyebright liquid in a ball bottle, wherein the specific preparation process comprises the following steps:
the method comprises the following steps: preparation of refined liquid of eyedrops
(1) Preparing a primary liquid: taking a proper amount of gynura bicolor, soaking the gynura bicolor in clear water for 20 minutes, putting the gynura bicolor into a marmite or a stainless steel pot, adding clear water for decocting twice, wherein the amount of the clear water added for the first time is 8-12 times of the weight of the gynura bicolor, the amount of the clear water added for the second time is 5-7 times of the weight of the gynura bicolor, the first decocting time is 35-45 minutes, the second decocting time is 15-25 minutes, removing dregs of a decoction through twice decocting, and combining the twice decocted liquid medicines to obtain a primary liquid;
(2) And (3) filtering: filtering the primary liquid obtained after extraction by adopting a 120-200 mesh filter screen to obtain filtrate;
(3) Refining, separating and concentrating: concentrating the filtrate to relative density of 1.0-1.1 to obtain concentrated solution, adding absolute ethanol to make the ethanol content of the Chinese medicinal liquid in the concentrated solution reach 70%, standing for 10-20 hours, collecting supernatant, and recovering ethanol to obtain refined stock solution of herba EZICAI;
(4) Preparing a refined solution: adding sodium benzoate into the refined stock solution, wherein the addition amount of the sodium benzoate is 0.1-0.5% of the weight of the refined stock solution, adding the sodium benzoate, stirring for dissolving, filtering by using a 200-mesh filter screen after uniformly stirring, and collecting the filtrate to obtain the refined liquid of the gynura divaricata for later use;
step two: treatment of ball bottles
(1) Initial washing: selecting a ball bottle with the specification of 10 ml, firstly adding a proper amount of drinking water to soak for 1 hour, then ultrasonically cleaning for 6 minutes by adopting an ultrasonic machine with the power of 200W, and taking out for later use after ultrasonic cleaning;
(2) Fine washing: cleaning the primarily cleaned ball bottle with purified water for 3 times;
(3) And (3) sterilization: putting the ball bottle after the fine cleaning into a stainless steel pot padded with gauze, boiling for 60 minutes, taking out, and then putting into an oven with the temperature of 80 ℃ for drying for 1 hour;
step three: filling and sterilizing
(1) Filling: filling the prepared refined herba Equiseti Arvinsis liquid in sterilized ball bottles, wherein the filling amount of each bottle is not less than 8.5ml;
(2) And (3) sterilization: placing the ball agent filled with the fine liquid of the Ezikia indica in an HJ-1405 ultraviolet sterilizer, adjusting the sterilization wavelength to 254nm, sterilizing for 60 minutes, sterilizing, and cooling to normal temperature to obtain the product of the Ezikia indica ball agent.
2. The method for preparing an eyedrop roll agent according to claim 1, wherein the method comprises the following steps: in the filtering process, a filter screen of 180-200 meshes is adopted for filtering, and the filtrate is collected after filtering.
3. The method for preparing an Ezikia ball agent according to claim 1, wherein the method comprises the following steps: in the process of preparing the refined liquid, the sodium benzoate is added according to the proportion of 0.3 percent of the weight of the refined stock solution, and the sodium benzoate is sodium benzoate solid.
4. An eyedrop roll agent prepared by the method of any one of claims 1 to 3.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210738158.3A CN115006476B (en) | 2022-06-28 | 2022-06-28 | Preparation method of eyebright rolling agent and eyebright rolling agent |
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| 冯甜 ; 徐文进 ; 马亚荣 ; 黄海艳 ; 杨文慧 ; 陈洪卫 ; 崔艳领 ; 巩江 ; 倪士峰 ; .眼子菜属药学研究概况.安徽农业科学.2014,第42卷(第36期),12898-12900. * |
| 洪开文.响应面法优化眼子菜多糖的提取工艺研究.《中国食品添加剂》.2020,(第9期),39-43. * |
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