CN115006284A

CN115006284A - Infant skin care composition and infant skin care product

Info

Publication number: CN115006284A
Application number: CN202210906031.8A
Authority: CN
Inventors: 刘文文; 王玉玲; 鲍玉; 任姝静; 王志华; 赵毅; 张晓鸥; 郭学平
Original assignee: Bloomage Biotech Co Ltd
Current assignee: Bloomage Biotech Co Ltd
Priority date: 2022-07-29
Filing date: 2022-07-29
Publication date: 2022-09-06

Abstract

An infant skin care composition is provided that includes hyaluronic acid or a salt thereof, cross-linked hyaluronic acid or a salt thereof, and glycerin. The macromolecular hyaluronic acid or the salt thereof and the cross-linked hyaluronic acid or the salt thereof in the infant skin care composition can form a thin, breathable and mild water-locking protective film on the surface of the skin of an infant, the protective film can lock the moisture of the skin, block the contact of the skin and external pollutants and does not hinder the skin respiration, and meanwhile, the obtained composition also has the effects of protecting and repairing the skin, so that the skin problem of the infant is relieved. In addition, the macromolecular sodium hyaluronate derivative can also prevent some unnecessary active ingredients from permeating into the skin and only remain in the horny layer to play the role of the active ingredients, so that the skin health of the infants is better protected.

Description

Infant skin care composition and infant skin care product

Technical Field

The application belongs to the technical field of cosmetics, and particularly relates to an infant skin care composition and an infant skin care product.

Background

Skin of infants is delicate, skin horniness layer is thin, and the thickness of the skin is only one tenth of that of adult skin, so that the skin has poor capacity of defending external force, the skin can be damaged by slight external force, and the risk of infection is increased after the skin is damaged, thereby causing the skin of infants to have symptoms of eczema, pruritus, redness and swelling and the like. The most basic method at present is to use moisturizers and emollients, which can keep the skin moist and soft, recover the elasticity and flexibility of the skin and help to reduce pruritus and scratch. However, the skin of infants is special, and the selected skin care product has to have higher safety than that of adult skin care products, namely, the washing and protecting product specially prepared for the infants is selected. The components are mild and harmless to the skin of babies, accord with the physiological characteristics of the skin of infants, and cannot cause skin anaphylaxis and toxic reaction.

Considering the skin characteristics of infants, a layer of protective film can be established on the skin surface of the infants, so that the skins of the infants can be protected from directly contacting the external environment, the contact between the damaged skins and external pollutants can be isolated to a certain extent, the moisture volatilization on the skin surface can be reduced, and the skin can be prevented from being dry; aiming at infants with skin problems, the protective film also has a certain protection and repair effect.

Disclosure of Invention

In order to solve the problems in the prior art, the application provides an infant skin care composition and an infant skin care product.

Specifically, the present application relates to the following aspects:

1. an infant skin care composition, wherein the infant skin care composition comprises hyaluronic acid or a salt thereof, cross-linked hyaluronic acid or a salt thereof, and glycerol.

2. The infant skin care composition according to item 1, wherein the hyaluronic acid or salt thereof is contained in an amount of 0.01 to 3.5 wt%, the cross-linked hyaluronic acid or salt thereof is contained in an amount of 0.1 to 3.5 wt%, and the glycerin is contained in an amount of 3 to 35 wt% in the infant skin care composition.

3. An infant skin care composition according to claim 1 wherein the hyaluronic acid or salt thereof has a molecular weight of 50-200 ten thousand Da.

4. The infant skin care composition according to claim 1, wherein the infant skin care composition further comprises a preservative ingredient, wherein the preservative ingredient is present in the infant skin care composition in an amount of 1% to 8%.

5. The infant skin care composition according to item 4, wherein the preservative comprises one or more of pentanediol, hexanediol, p-hydroxyacetophenone, butanediol, ethylhexylglycerin.

6. A method of making an infant skin care composition of items 1-5 comprising: mixing the hyaluronic acid or salt thereof, cross-linked hyaluronic acid or salt thereof, glycerin, and optionally preservative ingredients to obtain the infant skin care composition.

7. An infant skin care product comprising the infant skin care composition of any one of claims 1-5.

8. Use of the infant skin care composition of any one of claims 1-5 or the infant skin care product of claim 7 as a moisturizer.

9. Use of the infant skin care composition of any one of claims 1-5 or the infant skin care product of claim 7 as a uv-repair agent.

Compared with the prior art, the method has the following technical effects:

the macromolecular hyaluronic acid or the salt thereof and the cross-linked hyaluronic acid or the salt thereof in the infant skin care composition can form a thin, breathable and mild water-locking protective film on the surface of the skin of an infant, the protective film can lock the moisture of the skin, block the contact of the skin and external pollutants and does not hinder the breathing of the skin, and meanwhile, the obtained composition also has the effects of protecting and repairing the skin. Thereby alleviating skin problems in infants and young children. In addition, the macromolecular sodium hyaluronate derivative can also prevent some unnecessary active ingredients from permeating into the skin and only remain in the horny layer to play the role of the active ingredients, so that the skin health of the infants is better protected.

Drawings

FIG. 1 shows the appearance of the composition obtained in example 5 of the present application after film formation.

FIG. 2 shows a scanning electron micrograph of the composition obtained in example 5 of the present application after film formation.

FIG. 3 shows the skin moisturization of the composition obtained in example 5 of the present application, where (a) is the skin moisturization effect within 6 hours and (b) is the result of the transdermal water loss within 6 hours.

Detailed Description

The present application is further described below in conjunction with the following examples, which are intended to be illustrative and explanatory only and are not restrictive of the application.

Unless defined otherwise, technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art. Although methods and materials similar or equivalent to those described herein can be used in experimental or practical applications, the materials and methods are described below. In case of conflict, the present specification, including definitions, will control, and the materials, methods, and examples are illustrative only and not intended to be limiting. The present application is further described with reference to the following specific examples, which should not be construed as limiting the scope of the present application.

In view of the characteristics of infant skin, the present application provides an infant skin care composition comprising hyaluronic acid or a salt thereof, cross-linked hyaluronic acid or a salt thereof, and glycerin.

The hyaluronic acid is a component contained in human body itself, and is mucopolysaccharide substance composed of disaccharide repeating unit of N-acetylglucosamine and glucuronic acid. Hyaluronic acid plays multiple physiological functions on human body due to its special molecular composition structure and physicochemical properties. Hyaluronic acid has a special water retention effect, and high molecular weight hyaluronic acid can form a film on the surface of skin to play a role in locking water. Wherein the salt of hyaluronic acid can be sodium salt, potassium salt, zinc salt, calcium salt, magnesium salt, etc. of hyaluronic acid.

In some embodiments, the hyaluronic acid or salt thereof has a molecular weight of 50-200 ten thousand Da. For example 50, 60, 70, 80, 90, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, preferably 80-150, ten thousand Da.

The cross-linked hyaluronic acid or a salt thereof in the present application refers to a derivative of hyaluronic acid or a salt thereof, which is chemically modified by a cross-linking agent to link linear hyaluronic acid molecules into a spatial network structure.

The crosslinking method can be carried out by conventional methods. The cross-linked hyaluronic acid or the salt thereof is used as an ultra-high molecular weight polymer, has a unique spatial network structure, can form a long-acting biological protective film on the surface of skin, and has multiple functions of water locking, water storage, isolation, protection and the like.

When the hyaluronic acid or the salt thereof and the cross-linked hyaluronic acid or the salt thereof are used in combination, a thin, breathable and mild water-locking protective film can be formed on the surface of the skin of an infant, the protective film can lock the moisture of the skin, block the contact of the skin and external pollutants, and does not hinder the breathing of the skin, and meanwhile, the obtained composition also has the effects of protecting and repairing the skin.

In some embodiments, the hyaluronic acid or salt thereof is present in the infant skin care composition in an amount of 0.01-3.5 wt%, e.g., can be 0.01 wt%, 0.02 wt%, 0.03 wt%, 0.04 wt%, 0.05 wt%, 0.1 wt%, 0.2 wt%, 0.3 wt%, 0.4 wt%, 0.5 wt%, 0.6 wt%, 0.7 wt%, 0.8 wt%, 0.9 wt%, 1 wt%, 1.1 wt%, 1.2 wt%, 1.3 wt%, 1.4 wt%, 1.5 wt%, 1.6 wt%, 1.7 wt%, 1.8 wt%, 1.9 wt%, 2 wt%, 2.1 wt%, 2.2 wt%, 2.3 wt%, 2.4 wt%, 2.5 wt%, 2.6 wt%, 2.7 wt%, 2.8 wt%, 2.9 wt%, 3 wt%, 3.1 wt%, 3.2 wt%, 3.4 wt%, 3.5 wt%; the content of the crosslinked hyaluronic acid or a salt thereof is 0.1 to 3.5 wt%, and for example, may be 0.1 wt%, 0.2 wt%, 0.3 wt%, 0.4 wt%, 0.5 wt%, 0.6 wt%, 0.7 wt%, 0.8 wt%, 0.9 wt%, 1 wt%, 1.1 wt%, 1.2 wt%, 1.3 wt%, 1.4 wt%, 1.5 wt%, 1.6 wt%, 1.7 wt%, 1.8 wt%, 1.9 wt%, 2 wt%, 2.1 wt%, 2.2 wt%, 2.3 wt%, 2.4 wt%, 2.5 wt%, 2.6 wt%, 2.7 wt%, 2.8 wt%, 2.9 wt%, 3 wt%, 3.1 wt%, 3.2 wt%, 3.3 wt%, 3.4 wt%, 3.5 wt%; the content of the glycerol is 3 to 35% by weight, and for example, may be 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, 21%, 22%, 23%, 24%, 25%, 26%, 27%, 28%, 29%, 30%, 31%, 32%, 33%, 34%, 35% by weight.

In some embodiments, in the infant skin care composition, the hyaluronic acid or salt thereof is present in an amount of 0.05 to 3 wt%, the cross-linked hyaluronic acid or salt thereof is present in an amount of 0.5 to 2.5 wt%, and the glycerin is present in an amount of 5 to 30 wt%.

In some embodiments, in the infant skin care composition, the hyaluronic acid or salt thereof is present in an amount of 0.5 to 2.5 wt%, the cross-linked hyaluronic acid or salt thereof is present in an amount of 1 to 2.5 wt%, and the glycerin is present in an amount of 5 to 30 wt%.

In some embodiments, in the infant skin care composition, the hyaluronic acid or salt thereof is present in an amount of 0.5 to 1.5 wt%, the cross-linked hyaluronic acid or salt thereof is present in an amount of 1 to 2 wt%, and the glycerin is present in an amount of 10 to 20 wt%.

Further, the infant skin care composition may further comprise a preservative ingredient in an amount of 1% to 8%, such as 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8% in the infant skin care composition.

Wherein the antiseptic component comprises one or more of pentanediol, hexanediol, p-hydroxyacetophenone, butanediol and ethylhexyl glycerin. The pentanediol, the hexanediol, the p-hydroxyacetophenone, the butanediol and the ethylhexyl glycerin are mild and non-irritant safe substances, and the preservative composed of the components is different from the conventional preservative, so that the preservative has the functions of moisturizing and preserving on one hand, and is suitable for infants.

In some embodiments, the infant skin care composition can be further formulated into an infant skin care product in an amount of 0.5 to 6 wt%, for example, 0.5 wt%, 0.6 wt%, 0.7 wt%, 0.8 wt%, 0.9 wt%, 1.0 wt%, 1.5 wt%, 2.0 wt%, 2.5 wt%, 3.0 wt%, 3.5 wt%, 4.0 wt%, 4.5 wt%, 5.0 wt%, 6 wt% of the infant skin care product.

In a particular embodiment, the infant skin care composition consists of hyaluronic acid or a salt thereof, cross-linked hyaluronic acid or a salt thereof, glycerin, a preservative ingredient, and water. Further, the molecular weight of hyaluronic acid or a salt thereof and the contents of the respective components are as described above. The composition is simple in components, mild and non-irritant, has a moisturizing effect and a protection effect on ultraviolet, and is very suitable for infants. When the composition is used, a thin, breathable, mild and water-locking net-shaped protective film can be formed on the surface of the skin of an infant, skin breathing is benefited, particularly damaged skin can be isolated, external pollution components can be isolated, and active components act on the surface layer of the skin, so that the repair of the damaged skin is accelerated.

Examples

The experimental methods used in the following examples are all conventional methods, unless otherwise specified.

Materials, reagents and the like used in the following examples are commercially available, unless otherwise specified, and sodium hyaluronate used is commercially available from Huaxi Biotech Ltd.

Example 1

0.1g of sodium hyaluronate with the molecular weight of 90 ten thousand Da, 0.5g of cross-linked sodium hyaluronate, 15g of glycerol, 5g of pentanediol and 0.5g of hexanediol are weighed according to the proportion. Dispersing sodium hyaluronate and a sodium hyaluronate cross-linked polymer in glycerol, pentanediol and hexanediol, stirring and dispersing uniformly, adding purified water to make the total mass of the mixture be 100g, and stirring and dissolving uniformly to obtain the composition.

Examples 2 to 5

Examples 2-5 differ from example 1 in the amount of sodium hyaluronate and cross-linked sodium hyaluronate. Specifically, in example 2, the amount of sodium hyaluronate was 0.5g and the amount of crosslinked sodium hyaluronate was 1 g. In example 3, the amount of sodium hyaluronate was 1g and the amount of crosslinked sodium hyaluronate was 0.5 g. In example 4, the amount of sodium hyaluronate was 1.5g and the amount of crosslinked sodium hyaluronate was 1 g. In example 5, the amount of sodium hyaluronate was 1g and the amount of crosslinked sodium hyaluronate was 1.5 g.

Examples 6 to 10

Examples 6-10 differ from example 5 only in the amount of sodium hyaluronate. The amount of sodium hyaluronate in example 6 was 0.05g, the amount of sodium hyaluronate in example 7 was 0.5g, the amount of sodium hyaluronate in example 8 was 1.5g, the amount of sodium hyaluronate in example 9 was 2.5g, and the amount of sodium hyaluronate in example 10 was 3.5 g.

Examples 11 to 13

Examples 11-13 differ from example 5 only in the molecular weight of the sodium hyaluronate. Specifically, the molecular weight of sodium hyaluronate in example 11 is 150 ten thousand Da, the molecular weight of sodium hyaluronate in example 12 is 50 ten thousand Da, and the molecular weight of sodium hyaluronate in example 13 is 200 ten thousand Da.

Examples 14-16 differ from example 5 only in the amount of cross-linked sodium hyaluronate. Specifically, the amount of the crosslinked sodium hyaluronate in example 14 was 2.0g, the amount of the crosslinked sodium hyaluronate in example 15 was 2.5g, and the amount of the crosslinked sodium hyaluronate in example 16 was 3.5 g.

Examples 17 to 19

Examples 17-19 differ from example 5 only in the amount of glycerol. Specifically, 5.0g of glycerin was used in example 17, 20.0g of glycerin was used in example 18, and 30.0g of glycerin was used in example 19.

Example 20

Example 20 differs from example 5 only in that no pentanediol or hexanediol was added.

Comparative example 1

Comparative example 1 differs from example 5 in that no cross-linked sodium hyaluronate was added.

Comparative example 2

Comparative example 2 differs from example 5 in that sodium hyaluronate was not added.

Comparative example 3

Comparative example 3 differs from example 5 only in the amount of glycerol, which is 40.0g in comparative example 3.

The conditions of the above examples and comparative examples are shown in table 1.

TABLE 1 Condition of different examples and comparative examples

For the film forming properties of table 1, the film forming properties, which can be characterized by elasticity and toughness, were evaluated by stretching the films formed from the compositions of examples and comparative examples, measuring the tensile break length and the time to recover the original shape after stretching. The elasticity is the property that deformation occurs under the action of external force, and the deformation can completely disappear and the original shape can be recovered after the external force is removed. Specific values of elasticity in the present application are expressed as a percentage of the original length of the film when the film is stretched and when the stretching force is lost, the length after recovery. Toughness refers to the property of softness, firmness, and difficult fracture and breakage. The specific value of toughness in the present application is expressed as a percentage of the original length of a film formed by stretching, when the film breaks.

Specifically, specific meanings of toughness and elasticity in table 1 are as follows:

++++: the toughness is more than 150 percent, and the elasticity is more than 91 percent;

+++: representing the toughness of 145-150%, and the elasticity of 83% -91%;

++: representing 140% of toughness and 145% of elasticity, and 77% -83%;

+: representing 130% of toughness and 140% of elasticity, and 71% -77%;

-: representing the toughness of 120-;

- -: representing 110-120% of toughness and 66-68% of elasticity;

- - -: the toughness is 110% or less and the elasticity is 66% or less.

The appearance of the film formed from the composition prepared in example 5 is shown in FIG. 1, and the microporous structure is shown in FIG. 2.

Wherein the preparation process of the film forming composition comprises the following steps: the prepared composition was dropped into a rectangular (5 x 1 x 0.2mm) mold using a dropper to ensure that the liquid level was flush with the edge of the mold, the air bubbles were removed with the dropper, the resulting mixture was placed in a watch glass, and after standing at room temperature for 12 hours, the mold was removed, and the resulting film was removed with tweezers and observed.

Test example 1 moisture retention Performance test

The compositions prepared in examples 1, 5, 10, 16 and comparative examples 1-2 were added to emulsions whose formulations are shown in table 2.

TABLE 2

Blank emulsion: preparing blank emulsion according to the components in the table 2, stirring and dissolving the components of the A phase and the B phase uniformly at 80-90 ℃, pouring the A phase into the B phase, homogenizing for 3min, and naturally cooling to room temperature to obtain the blank emulsion.

And (3) testing the emulsion: on the basis of the blank emulsion, 2 wt% of the compositions prepared in examples 1, 5, 10 and 16 and comparative examples 1-2 were added and stirred uniformly.

20 μ L of the sample (i.e., blank and test) was applied evenly to the forearm of the arm at 3 x 3cm ² Sample area, randomly set blankAnd the sample area, massage to absorption. The skin was tested at different time points using a moisture meter and a water loss dispenser.

All tests are carried out in a constant temperature and humidity room, the temperature is 21 +/-1 ℃, the humidity is 50 +/-5 percent, and the tests can be carried out after the room is statically sitting for at least 30 minutes before each test.

Moisture growth rate%

The results of the moisture retention and the transdermal moisture loss of each of the test lotions are shown in table 3, in which the transdermal moisture loss ratio (% (amount of transdermal moisture loss of skin at time t applied-transdermal moisture loss of skin before application)/transdermal moisture loss of skin before application × 100%:

TABLE 3

FIG. 3 is the results of moisture retention and transdermal water loss of example 5, wherein (a) is the skin moisture retention within 6 hours and (b) is the transdermal water loss within 6 hours, and the test emulsion is the test emulsion to which 2 wt% of the composition prepared in example 5 was added.

As can be seen from figure 3, the skin moisturisation of the test emulsions was significantly improved over the blank emulsion, which for example in example 5 showed an increase in moisture content of 102.16% at 0.5h, compared to 44.01% for the blank emulsion. At 6.0 hours, the increase in moisture content of the test emulsion of example 5 remained at 13.75%, while the blank emulsion was only 5.8%. The moisture retention of each emulsion tended to decrease with time. In addition, transdermal water loss can also be seen, and each test emulsion obviously reduces the water loss on the surface of the skin, so that the test emulsion added with the composition prepared by the application has excellent long-acting water locking and moisturizing effects.

Test example 2 ultraviolet damage repair and protection test

UVA + UVB are adopted to irradiate damaged cells to form an ultraviolet damage model, and the activity of epidermal keratinocytes is evaluated by contacting the cells with a sample before and after irradiation to reflect the protection and repair capacity of the sample on ultraviolet damage.

The test method comprises the following steps:

1. ultraviolet damage repair

Taking HaCaT cells in logarithmic growth phase, and adding 5 x 10 ⁴ Inoculating to 96-well culture plate at density of 100 uL/well, placing in carbon dioxide incubator at 37 deg.C and 5% CO ₂ And culturing for 24h conventionally. Irradiating the preservative film with long-wave Ultraviolet (UVA) at 2000uW/cm ² The intensity of (2) is 30min, and the medium Ultraviolet (UVB) is 700uW/cm ² The intensity of (2) was irradiated for 3min, the normal control group was not irradiated, and the model group was irradiated. After irradiation, the old culture medium was discarded, the experimental group was added with a sample (the composition prepared in examples 1, 4, 5, 10, 11, and 16 and comparative examples 1-2) solution, the normal control group and the model group were added with a serum-free culture medium in each well at a volume of 100. mu.L, and after further culturing for 24 hours, the relative proliferation rate of cells was measured by the CCK-8 method.

2. Protection against ultraviolet damage

Taking HaCaT cells in logarithmic growth phase, and adding 5 x 10 ⁴ Inoculating to 96-well culture plate at density of 100 uL/well, placing in carbon dioxide incubator at 37 deg.C and 5% CO ₂ And culturing for 24h conventionally. The old culture medium was discarded after irradiation, a solution of 0.1% sample (composition prepared in examples 1, 4, 5, 10, 11, and 16 and comparative examples 1-2) was added to the experimental group, a serum-free culture medium was added to the normal control group and the model group, each well was 100. mu.L, and the irradiation was continued for 24 hours. Irradiating the preservative film covered by the cover, wherein the UVA is 2000uW/cm ² The intensity of the UV light is 30min, and the UVB light is 700uW/cm ² The intensity of (2) was irradiated for 3min, and the normal control group was not irradiated. The relative proliferation rate of the cells is detected by adopting a CCK-8 method.

The results of the uv damage repair test are shown in table 4, and the results of the uv damage protection test are shown in table 5.

TABLE 4

TABLE 5

As can be seen from the data in the table, the composition of the present application has a significant UV damage repairing and protecting effect, and the composition of example 5 has the best UV damage repairing and protecting effect. Therefore, the composition can be applied to infant products to effectively improve skin problems.

Claims

1. An infant skin care composition comprising hyaluronic acid or a salt thereof, cross-linked hyaluronic acid or a salt thereof, and glycerin.

2. The infant skin care composition according to claim 1, wherein the hyaluronic acid or salt thereof is present in an amount of 0.01 to 3.5 wt.%, the cross-linked hyaluronic acid or salt thereof is present in an amount of 0.1 to 3.5 wt.%, and the glycerin is present in an amount of 3 to 35 wt.% in the infant skin care composition.

4. The infant skin care composition of claim 1 further comprising a preservative ingredient, wherein the preservative ingredient is present in the infant skin care composition in an amount of from 1% to 8%.

5. An infant skin care composition according to claim 4 wherein the preservative ingredients comprise one or more of pentanediol, hexanediol, p-hydroxyacetophenone, butanediol, ethylhexylglycerin.

6. A process for preparing an infant skin care composition according to any one of claims 1 to 5 comprising the steps of: mixing the hyaluronic acid or salt thereof, cross-linked hyaluronic acid or salt thereof, glycerin, and optionally preservative ingredients to obtain the infant skin care composition.

8. Use of an infant skin care composition according to any one of claims 1 to 5 or an infant skin care product according to claim 7 as a moisturizer.

9. Use of an infant skin care composition according to any one of claims 1 to 5 or of claim 7 as a uv-repairing agent.