CN114983897A

CN114983897A - Anti-dandruff shampoo containing plant active polypeptide and preparation method thereof

Info

Publication number: CN114983897A
Application number: CN202210759193.3A
Authority: CN
Inventors: 刘伟
Original assignee: Guangzhou Meisi Biotechnology Co ltd
Current assignee: Guangzhou Meisi Biotechnology Co ltd
Priority date: 2022-06-30
Filing date: 2022-06-30
Publication date: 2022-09-02
Anticipated expiration: 2042-06-30
Also published as: CN114983897B

Abstract

The invention discloses a plant active polypeptide anti-dandruff shampoo and a preparation method thereof, wherein the anti-dandruff shampoo comprises the following raw materials, by mass, 35-50 parts of water, 6-12 parts of antibacterial peptide, 10-14 parts of surfactant, 0.8-1.2 parts of thickener, 1.1-1.4 parts of polyquaternary ammonium salt, 0.9-1.3 parts of essence, 1.4-1.8 parts of cacumen biotae extract, 1.2-1.4 parts of fructus cnidii extract and 1.0-1.3 parts of radix sophorae flavescentis extract. The invention can inhibit harmful fungi on scalp such as malassezia, thereby solving the fundamental problem of scurf, thoroughly radically treating the scurf, avoiding relapse, and the antibacterial peptide has a lipid modified molecular structure, high bioavailability, rapid penetration and absorption and higher safety than common antibacterial agents.

Description

Anti-dandruff shampoo containing plant active polypeptide and preparation method thereof

Technical Field

The invention relates to the technical field of shampoo, and particularly relates to a plant active polypeptide anti-dandruff shampoo and a preparation method thereof.

Background

The shampoo is an essential daily necessity in life, and with the continuous improvement of living standard, people have higher and higher requirements on the shampoo. It is not only required to have good cleaning effect, but also required to have nursing and conditioning functions. China is the country with the highest shampoo production and sale in the world at present, the brands of shampoos in domestic markets exceed 3000, the market scale of the shampoos is estimated to exceed 200 million yuan, the annual average purchase frequency of household shampoos is 5, and the yield of finished Chinese shampoos reaches 40 million tons.

According to different use requirements, the main emphasis points of the shampoo are different, and dandruff increase, scalp itching, alopecia, withered and yellow hair and damaged hair are the most common hair problems. Dandruff is medically known as pityriasis capitis, a fungal skin disease caused by malassezia. The mass propagation of malassezia on the scalp causes the hyperproliferation of the stratum corneum of the scalp, thereby promoting the abnormal falling of stratum corneum cells in the form of white or gray scales, and the fallen scales are the dandruff.

An article entitled "introduction and formulation of piroctone Olamine (OCT)" published by Johnny conn on the relevant information website, which details that the existing anti-dandruff shampoo is often added with chemical synthetic substances such as zinc pyrithione or piroctone olamine in the shampoo;

although the use cost is low, the substances have a plurality of disadvantages, such as certain sensitization, irritation and instability, can not be well absorbed and utilized by users, have a common anti-dandruff effect, are easy to relapse after being stopped use, and have low use safety.

Disclosure of Invention

The invention aims to provide a plant active polypeptide anti-dandruff shampoo and a preparation method thereof, and aims to solve the problems in the background technology.

The technical scheme of the invention is realized as follows:

according to one aspect of the invention, an anti-dandruff shampoo is provided.

The anti-dandruff shampoo comprises the following raw materials in parts by weight:

35-50 parts of water, 6-12 parts of antibacterial peptide, 10-14 parts of surfactant, 0.8-1.2 parts of thickening agent, 1.1-1.4 parts of polyquaternary ammonium salt, 0.9-1.3 parts of essence, 1.4-1.8 parts of cacumen biotae extract, 1.2-1.4 parts of fructus cnidii extract and 1.0-1.3 parts of radix sophorae flavescentis extract.

Furthermore, the antibacterial peptide comprises the following raw materials in parts by mass of hexapeptide-5, nisin, fennel antibacterial peptide and chlorella polypeptide in a ratio of 1:1:1: 3.

According to another aspect of the present invention, there is provided a method for preparing a green algae polypeptide, comprising the steps of:

s101, cleaning green algae to remove impurities, and then placing the green algae into a tissue triturator to triturate tissues;

step S103, adding the mashed green algae in the step S101 into a 6% acetic acid solution, wherein the mass ratio of the green algae to the acetic acid solution is 1: 4, performing ultrasonic treatment in an ultrasonic generator, performing centrifugal filtration, and collecting supernatant;

step S105, then, a Sephadex G-50 gel column is adopted for filtration chromatography, and salts and other proteins can be separated;

and S107, further eluting and purifying the SephadexLH-50 gel column collection with activity by using RP-HPLC to obtain the green alga polypeptide, and refrigerating and drying the green alga polypeptide for later use.

Further, the power of the ultrasonic generator in step S103 is 200W-250W, wherein the time of ultrasonic extraction is 25min, the centrifugation speed is 10000-12000rpm, and the centrifugation time is 12-18 min.

Further, the ratio of the length to the diameter of the gel column in the step S105 is 60:1, and the elution speed in the step S107 is 1.1 ml/min.

Further, the surfactant is one or a mixture of more of coconut oil diethanolamide, betaine and sodium laureth sulfate.

Further, the thickening agent is one or a mixture of more of guar hydroxypropyl trimonium chloride and acrylic acid copolymer.

Further, the essence is conventional in the field, and can be milk essence, rose essence or the like.

According to another aspect of the invention, a preparation method of an anti-dandruff shampoo is provided, and comprises the following steps:

step S201, weighing all raw materials required by the anti-dandruff shampoo according to the parts by weight;

step S203, adding the weighed water into a container, heating in a water bath, and keeping the water temperature at 55-65 ℃;

step S205, sequentially adding a surfactant, the cacumen Platycladi extract, the fructus Cnidii extract and the radix Sophorae Flavescentis extract into a container, and fully mixing and stirring for 15-20min by adopting a mechanical stirring mode to obtain a mixed solution A;

step S207, adding hexapeptide-5, nisin, phenformin, green alga polypeptide, a thickening agent and polyquaternary ammonium salt into the mixed solution A in sequence, fully mixing and stirring for 25-30min, and finally adding essence, mixing and stirring for 5-8min to obtain mixed solution B;

and S209, homogenizing the obtained mixed solution B to obtain the anti-dandruff shampoo.

Further, the rotation speed of the mixing and stirring in the step S205 and the step S207 is 350 rpm.

The raw material components adopted by the invention are explained as follows:

hexapeptide-5 can effectively promote the regeneration of collagen, elastic fiber and hyaluronic acid, improve the water content of the skin, strengthen the thickness of the skin, reduce expression wrinkles and improve the health state of the skin;

nisin is a small peptide consisting of 34 amino acids and biologically synthesized by fermentation of protein raw materials. The nisin has stronger inhibiting effect on gram-positive bacteria including food spoilage caused by staphylococcus, streptococcus, micrococcus and the like and harmful bacteria to human health, can effectively inhibit scalp harmful fungi such as malassezia and the like, thereby solving the fundamental problem of scurf, has high bioavailability and can be quickly permeated and absorbed due to the lipid modified molecular structure;

the fennel antimicrobial peptide is an antimicrobial peptide, can effectively inhibit harmful fungi on scalps such as malassezia and the like, thereby solving the fundamental problem of scurf, has a lipid modified molecular structure, high bioavailability and can be quickly permeated and absorbed;

on one hand, the green alga polypeptide can quickly neutralize acidic substances in the body and make the pH value of body fluid be alkalescent; on the other hand, the method activates and repairs the atrophic cells in vivo, promotes the healthy growth of the cells, and ensures the stable environment in the organism, so that the body is full of vitality, and can effectively inhibit harmful fungi of scalps such as malassezia, and the like, thereby solving the fundamental problem of dandruff;

the polyquaternary ammonium salt is a strong cationic high molecular polymer, has good solubility in water, has certain deoiling, deodorizing and corrosion-retarding effects, has excellent conditioning performance, ensures that hair has unique wet combing performance, improves the skin affinity and the hair quality brightness;

the folium Platycladi extract is branch tip and leaf of Platycladus orientalis of Cupressaceae, and has effects of cooling blood, stopping bleeding, promoting hair growth and blackening hair, wherein the folium Platycladi extract can be purchased or prepared by the method of example 1 with application number CN 114470012A;

fructus Cnidii extract is also called wild carrot seed. The fructus Cnidii extract is extracted from fruit of fructus Cnidii of Umbelliferae, and has effects of clearing heat, eliminating dampness, dispelling pathogenic wind and relieving itching, and its effective components contain pinene, isovalerian borneol ester, parsley methyl ether, dihydrocarveol, bergapten, osthole, and isoanisic apigenin. Can be used as additive for health products and functional beverages, wherein fructus Cnidii extract can be obtained by purchasing or preparing according to application number CN103636683B example 1;

the radix Sophorae Flavescentis extract is an alkaloid extracted from radix Sophorae Flavescentis of Leguminosae, and comprises matrine, oxymatrine, and anageine. The raw material has tough root, coarse fibrous cross section, yellowish white color, light smell and bitter taste. Radix Sophorae Flavescentis is cold in nature, and has effects of clearing heat, eliminating dampness, and killing parasites. The sophora flavescens bath can clear damp heat in lower jiao, kill parasites and relieve itching, has good relieving effect on skin pruritus, can balance grease secretion, dredge and astringe pores, and clear toxin impurities in skin. The sophora flavescens extract is purchased or prepared, and the preparation method can be according to the method of example 1 with the application number of CN 103636683B.

Compared with the prior art, the invention has the beneficial effects that:

(1) the dandruff removing shampoo is characterized in that various antibacterial peptides such as hexapeptide-5, nisin, fenmu antibacterial peptide and chlorella polypeptide are added into the dandruff removing shampoo, so that harmful fungi of scaly fungi and the like can be inhibited, the fundamental problem of dandruff is solved, the dandruff removing shampoo can thoroughly and radically cure and avoid relapse, the antibacterial peptides are lipid modified molecular structures, the bioavailability is high, the penetration and absorption can be fast, the safety is higher than that of a common bacteriostatic agent, and the comprehensive, lasting, mild, dandruff removing and itching relieving effects without damaging scalp can be achieved through the synergistic cooperation of the various active antibacterial peptides;

(2) the Chinese arborvitae twig extract has the effects of cooling blood, stopping bleeding, growing hair, blackening hair and the like, the skin irritation is effectively reduced through reasonable compounding of various types of surfactants, the cleaning and decontamination effects are improved, the sophora flavescens extract has the effects of killing parasites, relieving itching, well relieving skin pruritus, balancing grease secretion, dredging and astringing pores, and removing toxin impurities in skin. The skin care cream contains rich herbal nutrition, promotes the growth and repair of damaged vascular nerve cells, recovers the activity of subcutaneous capillary cells, and has the effects of skin care and skin care as the skin takes on compact and smooth appearance;

(3) the added polyquaternium has good solubility in water, has certain oil removal, deodorization and corrosion-retarding functions, has excellent conditioning performance, enables the hair to have unique wet combing performance, and improves the skin affinity and the hair quality brightness;

(4) the shampoo product disclosed by the invention has good cleaning and nursing effects, has the functions of removing dandruff for a long time, controlling oil, relieving itching, relieving scalp and the like, reduces the irritation of the shampoo while ensuring the dandruff removing effect, avoids the defects of high irritation and poor conditioning effect commonly existing in the common dandruff removing shampoo, and expands the application range.

Drawings

FIG. 1 is a schematic flow chart of a method for preparing a chlorella polypeptide in anti-dandruff shampoo according to the present invention;

fig. 2 is a schematic flow chart of a preparation method of an anti-dandruff shampoo provided by the invention;

fig. 3 is a line graph showing the number of malassezia spores in example 1 and comparative example 5 in the preparation method of an anti-dandruff shampoo according to the present invention.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

According to one aspect of the invention, an anti-dandruff shampoo is provided.

The antibacterial peptide comprises the following raw materials, by mass, hexapeptide-5, nisin, Fennel antibacterial peptide and chlorella polypeptide in a ratio of 1:1:1: 3.

In order to clearly understand the technical scheme of the invention, the technical scheme of the invention is described in detail by specific examples.

Example 1

35g of water, 6g of antibacterial peptide, 10g of surfactant, 0.8g of thickening agent, 1.1g of polyquaternium, 0.9g of essence, 1.4g of cacumen biotae extract, 1.2g of fructus cnidii extract and 1.0g of radix sophorae flavescentis extract.

The antibacterial peptide comprises the following raw materials, by mass, 1g of hexapeptide-5, 1g of nisin, 1g of fennel antibacterial peptide and 3g of chlorella polypeptide.

Wherein the surfactant is coconut oil diethanolamide and betaine.

Wherein the thickening agent is guar hydroxypropyl trimethyl ammonium chloride.

The preparation method of the green alga polypeptide comprises the following steps:

step S101, cleaning green algae to remove impurities, and then putting the green algae into a tissue triturator to triturate tissues;

step S103, adding the mashed green algae in the step S101 into 6% acetic acid solution, wherein the mass ratio of the green algae to the acetic acid solution is 1: 4, performing ultrasonic treatment in an ultrasonic generator, performing centrifugal filtration, and collecting supernatant;

The preparation method of the anti-dandruff shampoo comprises the following steps:

step S203, adding 35g of weighed water into a container, and heating in a water bath to keep the water temperature at 55-65 ℃;

step S205, sequentially adding 10g of surfactant, 1.4g of cacumen biotae extract, 1.2g of fructus cnidii extract and 1.0g of radix sophorae flavescentis extract into a container, and fully mixing and stirring for 15-20min by adopting a mechanical stirring mode to obtain mixed liquor A;

step S207, sequentially adding 1g of hexapeptide-5, 1g of nisin, 1g of Fenugen antibacterial peptide, 3g of chlorella polypeptide, 0.8g of thickening agent and 1.1g of polyquaternium into the mixed solution A, fully mixing and stirring for 25-30min, and finally adding 0.9g of essence, mixing and stirring for 5-8min to obtain mixed solution B;

Example 2:

42.5g of water, 9g of antibacterial peptide, 12g of surfactant, 1.0g of thickening agent, 1.25g of polyquaternium, 1.1g of essence, 1.6g of cacumen biotae extract, 1.3g of fructus cnidii extract and 1.15g of radix sophorae flavescentis extract.

The antibacterial peptide comprises the following raw materials, by mass, 1.5g of hexapeptide-5, 1.5g of nisin, 1.5g of fennel antibacterial peptide and 4.5g of chlorella polypeptide.

Wherein the surfactant is composed of coconut oil diethanolamide and sodium laureth sulfate.

Wherein the thickener is an acrylic copolymer.

step S203, adding 42.5g of weighed water into a container, and heating in a water bath to keep the water temperature at 55-65 ℃;

step S205, adding 12g of surfactant, 1.6g of cacumen biotae extract, 1.3g of fructus cnidii extract and 1.15g of radix sophorae flavescentis extract into a container in sequence, and fully mixing and stirring for 15-20min by adopting a mechanical stirring mode to obtain a mixed solution A;

step S207, sequentially adding 1.5g of hexapeptide-5, 1.5g of nisin, 1.5g of Fenugen antibacterial peptide, 4.5g of green alga polypeptide, 1.0g of thickening agent and 1.25g of polyquaternium into the mixed solution A, fully mixing and stirring for 25-30min, and finally adding 1.1g of essence, mixing and stirring for 5-8min to obtain mixed solution B;

Example 3:

50g of water, 12g of antibacterial peptide, 14g of surfactant, 1.2g of thickening agent, 1.4g of polyquaternium, 1.3g of essence, 1.8g of cacumen biotae extract, 1.4g of fructus cnidii extract and 1.3g of radix sophorae flavescentis extract.

The antibacterial peptide comprises the following raw materials, by mass, 2g of hexapeptide-5, 2g of nisin, 2g of fennel antibacterial peptide and 6g of chlorella polypeptide.

Wherein the surfactant is coconut oil diethanolamide, betaine and sodium laureth sulfate.

Wherein the thickening agent is composed of guar hydroxypropyl trimonium chloride and acrylic acid copolymer.

step S101, cleaning the purchased fresh green algae to remove impurities, and then putting the green algae into a tissue triturator to triturate green algae tissues;

step S203, adding 50g of weighed water into a container, and heating in a water bath to keep the water temperature at 55-65 ℃;

step S205, adding 14g of surfactant, 1.8g of cacumen biotae extract, 1.4g of fructus cnidii extract and 1.3g of radix sophorae flavescentis extract into a container in sequence, and fully mixing and stirring for 15-20min by adopting a mechanical stirring mode to obtain a mixed solution A;

step S207, sequentially adding 2g of hexapeptide-5, 2g of nisin, 2g of Fenugen antibacterial peptide, 6g of chlorella polypeptide, 1.2g of thickening agent and 1.4g of polyquaternium into the mixed solution A, fully mixing and stirring for 25-30min, and finally adding essence, mixing and stirring for 5-8min to obtain mixed solution B;

Comparative example 1

Comparative example 1 differs from example 1 in that hexapeptide-5 is not contained in comparative example 1, and the other things are the same.

Comparative example 2

Comparative example 2 differs from example 1 in that comparative example 2 does not contain nisin, and the other things are the same.

Comparative example 3

Comparative example 3 differs from example 1 in that comparative example 3 does not contain the fenarimycin, and the rest is the same.

Comparative example 4

Comparative example 4 differs from example 1 in that comparative example 4 does not contain a green alga polypeptide, and the other things are the same.

Comparative example 5

Comparative example 5 differs from example 1 in that comparative example 5 does not contain hexapeptide-5, nisin, fumonisin, chlorella polypeptide, and the like.

In conclusion, by means of the technical scheme, the dandruff removing shampoo can be invented, scalp harmful fungi such as malassezia can be inhibited by adding various antibacterial peptides including hexapeptide-5, nisin, fenamins antibacterial peptide and chlorella polypeptide into the dandruff removing shampoo, so that the fundamental problem of dandruff is solved, the dandruff can be thoroughly cured, relapse is avoided, the antibacterial peptides are lipid modified molecular structures, the bioavailability is high, the penetration and absorption can be fast realized, the safety is higher than that of a common antibacterial agent, the comprehensive and lasting effects of removing dandruff and relieving itching without damaging scalp can be achieved through the synergistic cooperation of the various active antibacterial peptides, the aquatic product has good cleaning and caring effects, and meanwhile has the functions of removing dandruff, controlling, relieving itching, relieving scalp and the like, and the irritation of the shampoo is reduced while the dandruff removing effect is ensured, the defects of large irritation and poor conditioning effect commonly existing in common anti-dandruff shampoo are overcome, and the application range is expanded.

The numbers of malassezia spores on the surfaces of 8h, 12h and 16h after the application of the method for detecting malassezia scalosa in Chinese patent publication No. CN110806408A to users are shown in the following table 1.

As can be clearly seen from the above examples 1 and comparative examples 5 and also from fig. 3, the anti-dandruff shampoo of the present invention can inhibit the number of malassezia spores well and reduce the number of dandruff effectively by adding the antibacterial peptide and not adding the antibacterial peptide to the shampoo, and can achieve a good therapeutic effect, while as can be seen from comparative examples 1 to 4, the anti-dandruff shampoo of the present invention is the best solution because the anti-dandruff effect is affected by adding a small amount of the antibacterial peptide, wherein the number of the malassezia spores is increased.

It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.

Claims

1. The anti-dandruff shampoo is characterized by comprising the following raw materials in parts by weight:

2. The anti-dandruff shampoo according to claim 1, characterized in that the antibacterial peptide comprises the following raw materials, by mass, hexapeptide-5, nisin, fenugreek antibacterial peptide and chlorella polypeptide in a ratio of 1:1:1: 3.

3. The anti-dandruff shampoo as claimed in claim 2, wherein the preparation method of the green alga polypeptide comprises the following steps:

and S107, further eluting and purifying the SephadexLH-50 gel column collection with activity by using RP-HPLC to obtain the green algae polypeptide, and refrigerating and drying the green algae polypeptide for later use.

4. The method of claim 3, wherein the power of the ultrasonic generator in step S103 is 200W-250W, the ultrasonic extraction time is 25min, the centrifugation speed is 10000-12000rpm, and the centrifugation time is 12-18 min.

5. The method of claim 2, wherein the ratio of the length to the diameter of the gel column in step S105 is 60:1, and the elution rate in step S107 is 1.1 ml/min.

6. The anti-dandruff shampoo as claimed in claim 1, wherein the surfactant is one or more selected from coconut diethanolamide, betaine and sodium laureth sulfate.

7. The anti-dandruff shampoo as claimed in claim 1, characterized in that the thickener is one or more of guar hydroxypropyltrimonium chloride and acrylic copolymer.

8. The anti-dandruff shampoo as claimed in claim 1, characterized in that the essence is a conventional essence in the field, and is one of milk essence or rose essence.

9. A method for preparing an anti-dandruff shampoo, characterized in that it is used for preparing an anti-dandruff shampoo according to any one of claims 1 to 7, comprising the following steps:

step S205, sequentially adding a surfactant, the cacumen Platycladi extract, the fructus Cnidii extract and the radix Sophorae Flavescentis extract into the container, and fully mixing and stirring for 15-20min by adopting a mechanical stirring manner to obtain a mixed solution A;

and step S209, homogenizing the obtained mixed solution B to obtain the anti-dandruff shampoo.

10. The method for preparing an anti-dandruff shampoo as claimed in claim 9, wherein the mixing and stirring speed in steps S205 and S207 is 350 rpm.