CN114931094A - Sterile germination cultivation method and device for cymbidium plant seeds - Google Patents

Sterile germination cultivation method and device for cymbidium plant seeds Download PDF

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Publication number
CN114931094A
CN114931094A CN202210371324.0A CN202210371324A CN114931094A CN 114931094 A CN114931094 A CN 114931094A CN 202210371324 A CN202210371324 A CN 202210371324A CN 114931094 A CN114931094 A CN 114931094A
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China
Prior art keywords
cultivation
sealing ring
disinfection
driving motor
seed
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CN202210371324.0A
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Chinese (zh)
Inventor
吴晓杰
王兴裕
余明洪
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Chongqing Guangruite Biotechnology Co ltd
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Chongqing Guangruite Biotechnology Co ltd
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Priority to CN202210371324.0A priority Critical patent/CN114931094A/en
Publication of CN114931094A publication Critical patent/CN114931094A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/001Culture apparatus for tissue culture
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/20Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
    • Y02P60/21Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures

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  • Life Sciences & Earth Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Botany (AREA)
  • Environmental Sciences (AREA)
  • Pretreatment Of Seeds And Plants (AREA)

Abstract

The invention relates to the technical field of seed cultivation devices, in particular to an aseptic germination cultivation method and device for cymbidium plant seeds.

Description

Sterile germination cultivation method and device for cymbidium plant seeds
Technical Field
The invention relates to the technical field of seed cultivation devices, in particular to a method and a device for cultivating cymbidium plant seeds through aseptic germination.
Background
With the development of plant seed cultivation technology, nursery stocks directly growing on the land can not be seen in many nursery gardens, nursery stocks of different varieties and specifications all grow in different containers, and the container type seedling cultivation can realize annual production, save seeds, shorten the seedling cultivation period, facilitate the cultivation of high-quality strong seedlings and improve the transplanting survival rate.
At present, the cultivation of the seeds of the cymbidium plants is directly carried out in the air, and bacteria in the air can possibly cause certain influence on the seeds of the cymbidium plants, thereby influencing the survival rate of the seeds of the cymbidium plants.
Disclosure of Invention
The invention aims to provide a method and a device for cultivating cymbidium plant seeds through aseptic germination, which are used for cultivating the cymbidium plant seeds in an aseptic environment so as to increase the survival rate of the cymbidium plant seeds.
In order to achieve the purpose, the invention provides an aseptic germination cultivation device for cymbidium plant seeds, which comprises an incubator, a cultivation plate and a partition component, wherein the incubator is provided with an cultivation chamber and a disinfection chamber which are communicated with each other, one side of the disinfection chamber, which is far away from the cultivation chamber, is provided with an inlet, the cultivation plate is in sliding connection with the incubator, and the partition component is arranged inside the disinfection chamber;
the cut off the subassembly and include sealing ring and driving motor, driving motor fixed mounting be in the top of disinfection room, the sealing ring with driving motor's output fixed connection, the sealing ring is located driving motor's below, just the bottom of sealing ring with the artificial containers rotates and connects, the side interval of sealing ring is provided with first opening and second opening, first opening is located the sealing ring is kept away from one side of fostering the room, the second opening is located the sealing ring is close to one side of fostering the room, the inboard of sealing ring is provided with the disinfection subassembly.
The seed putting state is that the first opening of the sealing ring coincides with the inlet, the sealing ring separates the cultivation room from the disinfection room, so that a worker places the cymbidium plant seeds on the cultivation plate, the seed disinfection state is that the first opening of the sealing ring is staggered with the inlet, the sealing ring separates the cultivation room from the disinfection room, the disinfection room forms a closed state, the disinfection component works to disinfect the cymbidium plant seeds, the seed putting state is that the first opening is staggered with the inlet, but the second opening of the sealing ring is communicated with the cultivation room, so that the worker pulls the cultivation plate into the cultivation room through rubber sealing gloves, the seed cultivation state is similar to the seed disinfection state, but the disinfection component does not work at the moment, when the seeds of the cymbidium plants need to be cultivated, the driving motor is controlled to operate to enable the sealing ring to be in a seed putting state, workers place the seeds of the cymbidium plants on the cultivating plate, then the driving motor operates again to enable the sealing ring to be in a seed disinfection state, the disinfection component operates to disinfect the cymbidium plant seeds, the driving motor operates again after disinfection to enable the sealing ring to be in a seed in-place state, a worker stretches into the interior of the cultivation chamber through the rubber sealing gloves to pull the cultivation plate from the interior of the sealing ring to the interior of the cultivation chamber, the driving motor operates again to enable the sealing ring to be in a seed cultivation state, at the moment, the cultivation chamber is in a closed state, the orchid plant seeds are subjected to aseptic cultivation, so that the survival rate of the orchid plant seeds is increased.
Wherein, the breeding device is sprouted to aseptic of orchid plant seed still includes the rubber ring, the incubator has the holding tank, the rubber ring with incubator fixed connection, the rubber ring is located the inside of holding tank, the bottom of sealing ring with the top of rubber ring is laminated mutually.
The bottom of the sealing ring is in contact with the top of the rubber ring, so that the sealing performance of the contact part of the sealing ring and the incubator is improved.
Wherein, the bottom of artificial board is provided with spacing support, the artificial board has with spacing mouthful of spacing support looks adaptation, spacing support is located the inside of spacing mouthful.
Spacing support can slide in the inside of spacing mouthful, spacing support with spacing mouthful mutually support in order to restrict the home range of culture plate, make the culture plate can be better for the artificial containers slides
Wherein, the room of growing is kept away from be provided with fixed subassembly on the medial surface of disinfection room, the culture plate has the draw-in groove, fixed subassembly includes fixed box, rotating turret, first spring, presses the compression leg and block post, fixed box with artificial containers fixed connection, the rotating turret with fixed box rotates to be connected, the rotating turret is located the inside of fixed box, press the compression leg with the rotating turret is articulated, the block post with rotating turret fixed connection, the block post with press the compression leg about the axle center of rotating turret is central symmetry and sets up, the both ends of first spring respectively with the rotating turret with fixed box fixed connection, first spring is located the inside of fixed box to be located the rotating turret is close to one side of pressing the compression leg.
The rotating frame can be in the inside of fixed box rotates, press the post with the block post sets up respectively the both ends of rotating frame, press the post setting to be in the upside of rotating frame, the block post sets up the downside of rotating frame, just press the post to be located the outside of artificial containers, the block post is located the inboard of cultivation room, first spring setting is in the below of rotating frame, the block post can the block get into the inside of draw-in groove, and first spring is right the rotating frame supports, makes the rotating frame is difficult for rotating, thereby will the artificial containers is fixed, needs to remove during the artificial containers, presses the post, makes the rotating frame rotate, thereby makes the block post follow withdraw from in the draw-in groove, just can slide the artificial containers.
Wherein, the aseptic germination breeding device of orchid plant seed still includes the observation window, the observation window with incubator fixed connection, the observation window is located the top of growing chamber.
The top of the cultivation room is provided with the observation window so that workers can observe the internal condition of the cultivation room conveniently.
The invention also provides a cultivation method of the sterile germination cultivation device for the cymbidium plant seeds, which specifically comprises the following steps:
the driving motor operates to drive the sealing ring to be in a seed placing state, and workers place orchids seeds on the cultivation plate;
the driving motor operates again to enable the sealing ring to be in a seed disinfection state, and the disinfection component conducts disinfection and sterilization treatment on the cultivation plate;
the driving motor operates again to enable the sealing ring to be in a seed in-place state, a worker pulls the cultivation plate to the inside of the cultivation room and fixes the cultivation plate through the fixing component;
the driving motor operates again to enable the sealing ring to be in a seed cultivation state, so that the cultivation room is in an aseptic state, and aseptic cultivation is conducted on cymbidium plant seeds.
The invention relates to a method and a device for cultivating cymbidium plant seeds through aseptic germination, when the cymbidium plant seeds need to be cultivated, firstly controlling the driving motor to operate to enable the sealing ring to be in a seed putting-in state, enabling a worker to place the cymbidium plant seeds on a cultivating plate, then enabling the driving motor to operate again to enable the sealing ring to be in a seed disinfection state, enabling the disinfection component to operate to disinfect the cymbidium plant seeds, enabling the driving motor to operate again to enable the sealing ring to be in a seed placing state after disinfection is finished, enabling the worker to stretch into the cultivating chamber through rubber sealing gloves to pull the cultivating plate from the inside of the sealing ring to the inside of the cultivating chamber, enabling the driving motor to operate again to enable the sealing ring to be in a seed cultivating state, enabling the cultivating chamber to be in a closed state at the moment, and enabling the cymbidium plant seeds to be cultivated aseptically, thereby increasing the survival rate of the seeds of the cymbidium plants.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a schematic structural view of an aseptic germination cultivation apparatus for cymbidium plant seeds provided by the present invention.
FIG. 2 is a cross-sectional view of an aseptic germination and cultivation apparatus for cymbidium plant seeds provided by the present invention.
Fig. 3 is a cross-sectional view taken at a-a of fig. 2 in accordance with the present invention.
Fig. 4 is a cross-sectional view at B-B of fig. 2 provided by the present invention.
Fig. 5 is a partial enlarged view at C in fig. 2 provided by the present invention.
Fig. 6 is a partial enlarged view of fig. 3D provided by the present invention.
FIG. 7 is a flow chart of a method for cultivating the aseptic germination cultivation device for cymbidium plant seeds according to the present invention.
1-incubator, 11-incubator, 12-disinfection chamber, 13-inlet, 14-holding tank, 15-limit port, 2-cultivation plate, 21-limit bracket, 22-clamp groove, 3-partition component, 31-sealing ring, 311-first opening, 312-second opening, 313-positioning port, 314-connecting port, 32-driving motor, 4-disinfection component, 41-disinfection frame, 411-mounting port, 412-positioning bracket, 42-disinfector, 5-rubber ring, 6-fixing component, 61-fixing box, 62-rotating frame, 63-first spring, 64-pressing column, 65-clamping column, 7-observation window, 8-connecting component, 81-second spring, 82-U-shaped frame.
Detailed Description
Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the drawings are illustrative and intended to be illustrative of the invention and are not to be construed as limiting the invention.
In the description of the present invention, it is to be understood that the terms "length", "width", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on the orientations or positional relationships illustrated in the drawings, and are used merely for convenience in describing the present invention and for simplicity in description, and do not indicate or imply that the devices or elements referred to must have a particular orientation, be constructed in a particular orientation, and be operated, and thus, are not to be construed as limiting the present invention. In addition, in the description of the present invention, "a plurality" means two or more unless specifically defined otherwise.
Referring to fig. 1 to 6, the invention provides an aseptic germination cultivation apparatus for cymbidium plant seeds, comprising an incubator 1, a cultivation plate 2 and a partition assembly 3, wherein the incubator 1 is provided with an incubation chamber 11 and a sterilization chamber 12 which are communicated with each other, one side of the sterilization chamber 12 far away from the incubation chamber 11 is provided with an inlet 13, the cultivation plate 2 is connected with the incubator 1 in a sliding manner, and the partition assembly 3 is arranged inside the sterilization chamber 12;
partition subassembly 3 includes sealing ring 31 and driving motor 32, driving motor 32 fixed mounting be in the top of disinfection room 12, sealing ring 31 with driving motor 32's output fixed connection, sealing ring 31 is located driving motor 32's below, just sealing ring 31's bottom with incubator 1 rotates and connects, sealing ring 31's side interval is provided with first opening 311 and second opening 312, first opening 311 is located sealing ring 31 keeps away from one side of cultivation room 11, second opening 312 is located sealing ring 31 is close to one side of cultivation room 11, the inboard of sealing ring 31 is provided with disinfection subassembly 4.
In this embodiment, the cultivation plate 2 can slide in the cultivation room 11 and the disinfection room 12, the cultivation room 11 is provided with an operation opening, rubber sealing gloves are arranged in the operation opening to partition the cultivation room 11 from the outside, a worker can put his hand into the rubber sealing gloves to operate the cultivation plate 2 and the cymbidium plant seeds, the driving motor 32 can drive the sealing ring 31 to rotate, the first opening 311 and the second opening 312 are arranged on the side surface of the sealing ring 31 at intervals, the driving motor 32 can drive the sealing ring 31 to rotate to various states, the various states are seed putting-in, seed disinfection, seed positioning and seed cultivation respectively, the seed putting-in state is that the first opening 311 of the sealing ring 31 coincides with the inlet 13, and the sealing ring 31 partitions the cultivation room 11 and the disinfection room 12, so that the worker can place the cymbidium plant seeds on the cultivation plate 2, the seed disinfection state is that the first opening 311 of the sealing ring 31 is dislocated with the inlet 13, and the sealing ring 31 separates the cultivation chamber 11 from the disinfection chamber 12, so that the disinfection chamber 12 forms a closed state, the disinfection component 4 works to disinfect the cymbidium plant seeds, the seed in-place state is that the first opening 311 is dislocated with the inlet 13, but the second opening 312 of the sealing ring 31 is communicated with the cultivation chamber 11, so that the worker can pull the cultivation plate 2 into the cultivation chamber 11 through rubber sealing gloves, the seed cultivation state is similar to the seed disinfection state, but at this time, the disinfection component 4 does not work, when the cymbidium plant seeds need to be cultivated, the driving motor 32 is controlled to operate to make the sealing ring 31 in the seed placing state, the worker places the seeds of the cymbidium plants on the cultivating plate 2, then the driving motor 32 is operated again to put the sealing ring 31 in a seed disinfection state, the disinfection component 4 is operated to disinfect the seeds of the cymbidium plants, after the disinfection is completed, the driving motor 32 is operated again to put the sealing ring 31 in a seed in-place state, the worker stretches into the inside of the cultivating chamber 11 through rubber sealing gloves to pull the cultivating plate 2 from the inside of the sealing ring 31 to the inside of the cultivating chamber 11, the driving motor 32 is operated again to put the sealing ring 31 in a seed cultivation state, and at this moment, the cultivating chamber 11 is in a closed state to carry out aseptic cultivation on the seeds of the cymbidium plants, so that the survival rate of the seeds of the cymbidium plants is increased.
Further, orchid plant seed's aseptic germination breeding device still includes rubber ring 5, incubator 1 has holding tank 14, rubber ring 5 with incubator 1 fixed connection, rubber ring 5 is located holding tank 14's inside, the bottom of sealing ring 31 with the top of rubber ring 5 is laminated mutually.
In this embodiment, the rubber ring 5 is disposed inside the accommodating groove 14, and the bottom of the sealing ring 31 contacts the top of the rubber ring 5, thereby increasing the sealing performance at the contact position of the sealing ring 31 and the incubator 1.
Further, a limiting bracket 21 is arranged at the bottom of the cultivation plate 2, the cultivation box 1 is provided with a limiting opening 15 matched with the limiting bracket 21, and the limiting bracket 21 is positioned inside the limiting opening 15;
a fixing component 6 is arranged on one inner side surface of the culture chamber 11 far away from the disinfection chamber 12, the cultivating plate 2 has a catching groove 22, the fixing member 6 includes a fixing case 61, a rotating frame 62, a first spring 63, a pressing post 64 and a catching post 65, the fixed box 61 is fixedly connected with the incubator 1, the rotating frame 62 is rotatably connected with the fixed box 61, the rotating frame 62 is positioned inside the fixed box 61, the pressing column 64 is hinged with the rotating frame 62, the clamping column 65 is fixedly connected with the rotating frame 62, the clamping column 65 and the pressing column 64 are arranged in central symmetry around the axis of the rotating frame 62, both ends of the first spring 63 are respectively fixedly connected with the rotating frame 62 and the fixed box 61, the first spring 63 is located inside the fixed box 61 and on a side of the rotating frame 62 close to the pressing column 64.
In this embodiment, the limiting bracket 21 can slide inside the limiting opening 15, the limiting bracket 21 and the limiting opening 15 cooperate with each other to limit the moving range of the cultivation plate 2, so that the cultivation plate 2 can better slide relative to the cultivation box 1, the fixing component 6 for fixing the cultivation plate 2 is provided on the inner side surface of the cultivation room 11 to fix the cultivation plate 2 for cultivating the orchid plant seeds, the rotating frame 62 can rotate inside the fixing box 61, the pressing columns 64 and the engaging columns 65 are respectively provided at both ends of the rotating frame 62, the pressing columns 64 are provided at the upper side of the rotating frame 62, the engaging columns 65 are provided at the lower side of the rotating frame 62, and the pressing columns 64 are located at the outer side of the cultivation box 1, the engaging column 65 is located inside the incubation chamber 11, the first spring 63 is disposed below the rotating frame 62, the engaging column 65 can engage with the inside of the engaging groove 22, and the first spring 63 abuts against the rotating frame 62, so that the rotating frame 62 is not easy to rotate, thereby fixing the incubation plate 2, when the incubation plate 2 needs to be moved, the pressing column 64 is pressed to rotate the rotating frame 62, thereby allowing the engaging column 65 to withdraw from the engaging groove 22, and the incubation plate 2 can be slid.
Further, orchid plant seed's aseptic germination breeding device still includes observation window 7, observation window 7 with incubator 1 fixed connection, observation window 7 is located the top of growing chamber 11.
In the present embodiment, the observation window 7 is provided at the top of the incubation chamber 11 so that a worker can observe the inside of the incubation chamber 11.
Further, the sterilizing assembly 4 comprises a sterilizing rack 41 and a sterilizer 42, the sterilizing rack 41 is detachably connected with the sealing ring 31, the sterilizing rack 41 is provided with a mounting opening 411, and the sterilizer 42 is arranged inside the mounting opening 411;
the disinfection frame 41 is provided with a positioning bracket 412, the sealing ring 31 is provided with a positioning opening 313 matched with the positioning bracket 412, and after the disinfection frame 41 is connected with the sealing ring 31, the positioning bracket 412 is positioned in the positioning opening 313;
the inside of locating support 412 is provided with coupling assembling 8, be provided with connector 314 on the medial surface of location mouth 313, coupling assembling 8 includes second spring 81 and U-shaped frame 82, the both ends of second spring 81 respectively with the link with locating support 412 is fixed mutually, U-shaped frame 82 with link sliding connection, it is right disinfection frame 41 with the back is connected to sealing ring 31, the one end of U-shaped frame 82 is located connector 314's inside.
In this embodiment, the disinfection rack 41 is used for placing the disinfector 42, the disinfector 42 can disinfect the seeds of the cymbidium plant, when the disinfection rack 41 is installed, the positioning bracket 412 is inserted into the positioning opening 313 to position the disinfection rack 41, so as to facilitate the installation of the disinfection rack 41, the disinfection rack 41 is fixed with the sealing ring 31 through the connecting assembly 8, the U-shaped rack 82 can slide relative to the positioning bracket 412, the U-shaped rack 82 is supported by the second spring 81, so that one end of the U-shaped rack 82 enters the inside of the connecting opening 314, and the positioning bracket 412 is fixed inside the positioning opening 313, so as to fix the disinfection rack 41.
Referring to fig. 7, the present invention further provides a cultivation method using the above cultivation device for aseptic germination of cymbidium plant seeds, which specifically includes the following steps:
s1: the driving motor 32 operates to drive the sealing ring 31 to be in a seed placing state, and workers place orchids seeds on the cultivation plate 2;
s2: the driving motor 32 is operated again to enable the sealing ring 31 to be in a seed disinfection state, and the disinfection component 4 conducts disinfection and sterilization treatment on the cultivation plate 2;
s3: the driving motor 32 is operated again to put the sealing ring 31 in the seed seating state, and the worker pulls the cultivation plate 2 to the inside of the cultivation room 11 and fixes the cultivation plate 2 by the fixing member 6;
s4: the driving motor 32 is operated again to put the sealing ring 31 in a seed cultivation state to put the cultivation room 11 in an aseptic state to perform aseptic cultivation of the cymbidium plant seed.
When culturing cymbidium plant seeds, firstly controlling the driving motor 32 to operate to make the sealing ring 31 in a seed putting state, placing the cymbidium plant seeds on the culturing plate 2 by a worker, then operating the driving motor 32 again to make the sealing ring 31 in a seed disinfection state, operating the disinfection component 4 to disinfect the cymbidium plant seeds, operating the driving motor 32 again to make the sealing ring 31 in a seed placing state after the disinfection is completed, extending the worker into the culturing chamber 11 through rubber sealing gloves to pull the culturing plate 2 from the inside of the sealing ring 31 to the inside of the culturing chamber 11, fixing the culturing plate 2 through the fixing component 6, operating the driving motor 32 again to make the sealing ring 31 in a seed culturing state, and at the moment, keeping the culturing chamber 11 in a closed state, the orchid plant seeds are subjected to aseptic cultivation, so that the survival rate of the orchid plant seeds is increased.
While the invention has been described with reference to a preferred embodiment, it will be understood by those skilled in the art that various changes in form and detail may be made therein without departing from the spirit and scope of the invention as defined by the appended claims.

Claims (6)

1. An aseptic germination cultivation device for cymbidium plant seeds is characterized in that,
the cultivation device comprises an incubator (1), an incubation plate (2) and a partition component (3), wherein the incubator (1) is provided with an incubation chamber (11) and a disinfection chamber (12) which are communicated with each other, one side of the disinfection chamber (12), which is far away from the incubation chamber (11), is provided with an inlet (13), the incubation plate (2) is in sliding connection with the incubator (1), and the partition component (3) is arranged inside the disinfection chamber (12);
cut off subassembly (3) including sealing ring (31) and driving motor (32), driving motor (32) fixed mounting be in the top of disinfection room (12), sealing ring (31) with the output fixed connection of driving motor (32), sealing ring (31) are located the below of driving motor (32), just the bottom of sealing ring (31) with incubator (1) rotates and connects, the side interval of sealing ring (31) is provided with first opening (311) and second opening (312), first opening (311) are located sealing ring (31) are kept away from one side of cultivation room (11), second opening (312) are located sealing ring (31) are close to one side of cultivation room (11), the inboard of sealing ring (31) is provided with disinfection subassembly (4).
2. An aseptic germination cultivation apparatus for cymbidium plant seeds as claimed in claim 1,
orchid plant seed's aseptic germination breeding device still includes rubber ring (5), incubator (1) has holding tank (14), rubber ring (5) with incubator (1) fixed connection, rubber ring (5) are located the inside of holding tank (14), the bottom of sealing ring (31) with the top of rubber ring (5) is laminated mutually.
3. An aseptic germination cultivation apparatus for cymbidium plant seeds as claimed in claim 2,
the bottom of artificial board (2) is provided with spacing support (21), artificial board (1) have with spacing mouthful (15) of spacing support (21) looks adaptation, spacing support (21) are located the inside of spacing mouthful (15).
4. An aseptic germination cultivation apparatus for cymbidium plant seeds as claimed in claim 3,
a fixing component (6) is arranged on one inner side face of the cultivation room (11) far away from the disinfection room (12), the cultivation plate (2) is provided with a clamping groove (22), the fixing component (6) comprises a fixing box (61), a rotating frame (62), a first spring (63), a pressing column (64) and a clamping column (65), the fixing box (61) is fixedly connected with the cultivation box (1), the rotating frame (62) is rotatably connected with the fixing box (61), the rotating frame (62) is positioned in the fixing box (61), the pressing column (64) is hinged with the rotating frame (62), the clamping column (65) is fixedly connected with the rotating frame (62), the clamping column (65) and the pressing column (64) are arranged in central symmetry relative to the axis of the rotating frame (62), two ends of the first spring (63) are respectively and fixedly connected with the rotating frame (62) and the fixing box (61), the first spring (63) is positioned inside the fixed box (61) and on one side of the rotating frame (62) close to the pressing column (64).
5. An aseptic germination cultivation apparatus for cymbidium plant seeds as claimed in claim 4,
orchid plant seed's aseptic germination breeding device still includes observation window (7), observation window (7) with incubator (1) fixed connection, observation window (7) are located the top of growing chamber (11).
6. A method of using the apparatus for sterile germination cultivation of cymbidium plant seeds according to claim 5, comprising the following steps:
the driving motor (32) operates to drive the sealing ring (31) to be in a seed placing state, and workers place orchids seeds on the cultivation plate (2);
the driving motor (32) operates again to enable the sealing ring (31) to be in a seed disinfection state, and the disinfection component (4) conducts disinfection and sterilization treatment on the cultivation plate (2);
the driving motor (32) is operated again to enable the sealing ring (31) to be in a seed locating state, a worker pulls the cultivation plate (2) to the inside of the cultivation room (11), and the cultivation plate (2) is fixed through the fixing component (6);
the driving motor (32) operates again to enable the sealing ring (31) to be in a seed cultivation state, so that the cultivation room (11) is in a sterile state, and sterile cultivation is carried out on the cymbidium plant seeds.
CN202210371324.0A 2022-04-11 2022-04-11 Sterile germination cultivation method and device for cymbidium plant seeds Pending CN114931094A (en)

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CN202210371324.0A CN114931094A (en) 2022-04-11 2022-04-11 Sterile germination cultivation method and device for cymbidium plant seeds

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CN202210371324.0A CN114931094A (en) 2022-04-11 2022-04-11 Sterile germination cultivation method and device for cymbidium plant seeds

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