CN114929250A - 用于疗法的经工程化的细胞 - Google Patents

用于疗法的经工程化的细胞 Download PDF

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CN114929250A
CN114929250A CN202080091509.5A CN202080091509A CN114929250A CN 114929250 A CN114929250 A CN 114929250A CN 202080091509 A CN202080091509 A CN 202080091509A CN 114929250 A CN114929250 A CN 114929250A
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cell
receptor
cells
beta
rna
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G·格兰特·威尔斯戴德
J·二世·穆
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Editas Medicine Inc
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Editas Medicine Inc
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CN202080091509.5A 2019-12-18 2020-12-18 用于疗法的经工程化的细胞 Pending CN114929250A (zh)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201962950063P 2019-12-18 2019-12-18
US62/950,063 2019-12-18
US202063025735P 2020-05-15 2020-05-15
US63/025,735 2020-05-15
US202063115592P 2020-11-18 2020-11-18
US63/115,592 2020-11-18
PCT/US2020/066256 WO2021127594A1 (en) 2019-12-18 2020-12-18 Engineered cells for therapy

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CN114929250A true CN114929250A (zh) 2022-08-19

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US (1) US20230053028A1 (https=)
EP (1) EP4076479A4 (https=)
JP (1) JP2023507118A (https=)
KR (1) KR20220119063A (https=)
CN (1) CN114929250A (https=)
AU (1) AU2020405203A1 (https=)
CA (1) CA3164660A1 (https=)
IL (1) IL293946A (https=)
WO (1) WO2021127594A1 (https=)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN120174005A (zh) 2019-02-13 2025-06-20 比姆医疗股份有限公司 具有用于修饰靶标序列中核碱基的腺苷脱氨酶碱基编辑器的经修饰的免疫细胞
EP4034138A4 (en) 2019-09-27 2024-07-31 Beam Therapeutics, Inc. COMPOSITIONS AND METHODS FOR THE TREATMENT OF LIQUID CANCERS
CA3196831A1 (en) 2020-09-25 2022-03-31 Beam Therapeutics Inc. Fratricide resistant modified immune cells and methods of using the same
US11459372B2 (en) 2020-11-30 2022-10-04 Crispr Therapeutics Ag Gene-edited natural killer cells
US11661459B2 (en) 2020-12-03 2023-05-30 Century Therapeutics, Inc. Artificial cell death polypeptide for chimeric antigen receptor and uses thereof
KR20230118887A (ko) 2020-12-03 2023-08-14 센츄리 쎄라퓨틱스 인코포레이티드 유전자 조작 세포 및 이의 용도
AR124414A1 (es) 2020-12-18 2023-03-22 Century Therapeutics Inc Sistema de receptor de antígeno quimérico con especificidad de receptor adaptable
WO2022144632A1 (en) 2020-12-30 2022-07-07 Crispr Therapeutics Ag Compositions and methods for differentiating stem cells into nk cells
WO2023108107A2 (en) * 2021-12-10 2023-06-15 Beam Therapeutics Inc. Modified immune cells and methods of using the same
CN117384853B (zh) * 2022-07-11 2025-12-19 士泽生物医药（苏州）有限公司 一种表达pvr的通用型细胞及其制备方法
AU2023356353A1 (en) * 2022-10-05 2025-05-08 Garuda Therapeutics, Inc. Modified hematopoietic stem cells and progenies thereof
WO2025166325A1 (en) * 2024-02-02 2025-08-07 Editas Medicine, Inc. MODIFIED GUIDE RNAs
WO2025238593A1 (en) * 2024-05-16 2025-11-20 Fondazione Istituto Italiano Di Tecnologia DESIGN OF SYNTHETIC miRNAS TO RESTORE THE CORRECT GENE DOSAGE OF GENES ASSOCIATED WITH T LYMPHOCYTE EXHAUSTION

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018081470A1 (en) * 2016-10-27 2018-05-03 Intima Bioscience, Inc. Viral methods of making genetically modified cells
WO2019089884A2 (en) * 2017-11-01 2019-05-09 Editas Medicine, Inc. Methods, compositions and components for crispr-cas9 editing of tgfbr2 in t cells for immunotherapy

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6812339B1 (en) * 2000-09-08 2004-11-02 Applera Corporation Polymorphisms in known genes associated with human disease, methods of detection and uses thereof
US20030073623A1 (en) * 2001-07-30 2003-04-17 Drmanac Radoje T. Novel nucleic acid sequences obtained from various cDNA libraries
JP2008307007A (ja) * 2007-06-15 2008-12-25 Bayer Schering Pharma Ag 出生後のヒト組織由来未分化幹細胞から誘導したヒト多能性幹細胞
US9233926B2 (en) * 2008-09-17 2016-01-12 Sanford-Burnham Medical Research Institute Compounds for stem cell differentiation
EP3495497B1 (en) * 2011-04-28 2021-03-24 Life Technologies Corporation Methods and compositions for multiplex pcr
KR20260004568A (ko) * 2016-04-19 2026-01-08 더 브로드 인스티튜트, 인코퍼레이티드 신규한 crispr 효소 및 시스템
EP3612210A4 (en) * 2017-04-19 2021-01-27 Board Of Regents, The University Of Texas System MANIPULATED ANTIGEN RECEPTORS EXPRESSING IMMUNE CELLS
BR112020006601A2 (pt) * 2017-10-02 2020-12-08 Genedit Inc. Rna guia de cpf1 modificado
KR102907245B1 (ko) * 2018-03-14 2026-01-05 에디타스 메디신, 인코포레이티드 혈색소병증 치료를 위한 시스템 및 방법
EP3790562A4 (en) * 2018-05-11 2022-01-12 The Regents of the University of California MODIFICATION OF IMMUNE CELLS TO INCREASE ACTIVITY
MX2021006208A (es) * 2018-11-28 2021-10-01 Univ Texas Edición por multiplexación del genoma de células inmunitarias para mejorar la funcionalidad y resistencia al entorno supresor.

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018081470A1 (en) * 2016-10-27 2018-05-03 Intima Bioscience, Inc. Viral methods of making genetically modified cells
WO2019089884A2 (en) * 2017-11-01 2019-05-09 Editas Medicine, Inc. Methods, compositions and components for crispr-cas9 editing of tgfbr2 in t cells for immunotherapy

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
"A2AR Adenosine Signaling Suppresses Natural Killer Cell Maturation in the Tumor Microenvironment", CANCER RES, vol. 78, no. 4, 15 February 2018 (2018-02-15) *
ZHU H.等: "CISH DELETION COMBINED WITH NOTCH ACTIVATION LEADS TO PRODUCTION OF HYPER-ACTIVE HUMAN NATURAL KILLER CELLS DERIVED FROM HUMAN PLURIPOTENT STEM CELLS", EXPERIMENTAL HEMATOLOGY, vol. 64, 31 August 2018 (2018-08-31), pages 76, XP085449420, DOI: 10.1016/j.exphem.2018.06.259 *
ZHU, H.等: "Deletion of CISH in Human Pluripotent Stem Cell- Derived Natural Killer Cells Enhances Anti- Tumor Activity Via Metabolic Reprogramming", BLOOD, vol. 134, no. 1, 13 November 2019 (2019-11-13), pages 1 - 2 *

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IL293946A (en) 2022-08-01
EP4076479A1 (en) 2022-10-26
CA3164660A1 (en) 2021-06-24
WO2021127594A1 (en) 2021-06-24
EP4076479A4 (en) 2024-05-22
AU2020405203A1 (en) 2022-07-14
JP2023507118A (ja) 2023-02-21
US20230053028A1 (en) 2023-02-16
KR20220119063A (ko) 2022-08-26

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