CN114874883A - Separation of nanometer fat is drawed and concentrated purification device - Google Patents
Separation of nanometer fat is drawed and concentrated purification device Download PDFInfo
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- CN114874883A CN114874883A CN202210599625.9A CN202210599625A CN114874883A CN 114874883 A CN114874883 A CN 114874883A CN 202210599625 A CN202210599625 A CN 202210599625A CN 114874883 A CN114874883 A CN 114874883A
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/04—Cell isolation or sorting
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/12—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by pressure
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/12—Purification
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Abstract
The invention relates to the technical field of medical instruments, in particular to a device for separating, extracting, concentrating and purifying nano fat, which comprises a fat suction pipe, a separation and purification pipe and a negative pressure suction device, wherein the fat suction pipe is positioned above the separation and purification pipe and is communicated with the separation and purification pipe through a Ruhr three-way valve, a filter screen cylinder is installed in the separation and purification pipe, the negative pressure suction device comprises a core rod, a piston and a negative pressure suction interface, the core rod extends into the filter screen cylinder from the bottom of the separation and purification pipe, the upper part of the core rod is provided with a suction hole, the piston is fixedly installed at the top of the core rod, and the negative pressure suction interface is positioned at the bottom of the core rod and extends out of the separation and purification pipe. The device provided by the invention has the advantages of simple structure, small volume, light weight and convenient and labor-saving operation, and the prepared nano active fat has the advantages of high purity and good activity and can reduce adverse reaction caused by injection.
Description
Technical Field
The invention relates to the technical field of medical instruments, in particular to a device for separating, extracting, concentrating and purifying nano fat.
Background
The nano fat is prepared by primarily purifying the taken fat, destroying mature fat cells, namely fat cells containing large-particle oil drops, and is not easy to survive after being filled, and the large-particle oil drops are easy to cause inflammatory reactions such as swelling and the like. The survival rate of the fat cells with smaller volume is higher than that of the fat cells with smaller volume after filling, the diameter of the fat clusters with nano fat particles is usually only 0.4-0.6mm, and the nano fat is widely applied to the field of fat transplantation at present.
When the nano adipose cells are separated, extracted, concentrated and purified, a complex system is often required for processing, the system structure is complex and heavy, the operation procedure is complex, the extraction cost is high, the efficiency is low, and the extracted active adipose tissues containing adipose stem cells and the like have low purity and poor activity and are easy to cause adverse reactions during injection.
Disclosure of Invention
The technical problem to be solved by the invention is to provide a separation, extraction, concentration and purification device for nano fat, which has the advantages of simple structure, small volume, light weight, convenient and labor-saving operation, and the prepared nano active fat has the advantages of high purity and good activity and can reduce adverse reaction caused by injection. The invention is realized by the following technical scheme:
the utility model provides a separation of nanometer fat is drawed and is concentrated purification device, its includes fat suction tube, separation purification pipe and negative pressure suction device, the fat suction tube is located separation purification pipe top and with through luer three-way valve intercommunication between the separation purification pipe, install the filter screen section of thick bamboo in the separation purification pipe, negative pressure suction device includes core bar, piston and negative pressure suction interface, the core bar stretches into in the filter screen section of thick bamboo from separation purification pipe bottom, and core bar upper portion has seted up the suction opening, piston fixed mounting is in the core bar top, negative pressure suction interface is located the core bar bottom and stretches out separation purification pipe.
Furthermore, the fat suction pipe comprises a suction pipe body and a suction pipe base, the upper part of the suction pipe body is provided with a fat suction hole, the suction pipe base comprises a connecting pipe and a clamping support, the clamping support is clamped at the top of the Ruhr three-way valve, and the connecting pipe fixedly penetrates through the clamping support and is communicated with the suction pipe body and the Ruhr three-way valve.
Further, separation and purification pipe top is equipped with collects the mouth, and bottom fixed mounting has the shrouding, luer three-way valve is installed on the collection mouth of separation and purification pipe.
Further, a press hand is fixedly arranged on the core bar above the negative pressure suction port.
Preferably, the Ruhr three-way valve is provided with a Ruhr interface.
Preferably, the negative pressure suction port is a threaded bevel port.
Preferably, the fat suction hole is plural.
The invention has the advantages of
The device for separating, extracting, concentrating and purifying the nano fat provided by the invention has the following advantages: the device has the advantages of simple structure, small volume, light weight, convenient and labor-saving operation, can perform efficient separation and extraction on the basis of keeping the activity of the nano adipose tissue, improve the bioactivity of the nano adipose tissue through purification and concentration, and reduce the adverse reaction caused by the injection of the nano adipose tissue.
Drawings
FIG. 1 is a schematic diagram of the suction state of adipose tissues according to the present invention;
FIG. 2 is a schematic diagram of the structure of the fat tissue in the opposite flushing state according to the present invention;
in the figure, 1, a fat suction hole, 2, a suction pipe body, 3, a suction pipe base, 4, a Ruhr three-way valve, 5, a collection port, 6, a separation and purification pipe, 7, a filter screen cylinder, 8, a piston, 9, a suction hole, 10, a core rod, 11, a sealing plate, 12, a press hand, 13, a negative pressure suction port, 14, a first Ruhr syringe, 15, a second Ruhr syringe, 16, a clamping support and 17 are connected pipes.
Detailed Description
The utility model provides a separation of nanometer fat is drawed and is concentrated purification device, its includes fat suction tube, separation and purification pipe 6 and negative pressure suction device, the fat suction tube be located separation and purification pipe top and with separate and purify between the pipe through luer three-way valve 4 intercommunication, the fat suction tube can get into human adipose tissue, take out adipose tissue and send into in the separation and purification pipe through luer three-way valve through adipose tissue.
The separation and purification pipe is internally provided with a filter screen cylinder 7 which can filter and separate the separated and purified tissue and the separated liquid oil drops. The negative pressure suction device comprises a core rod 10, a piston 8 and a negative pressure suction interface 13, the core rod extends into the filter screen cylinder from the bottom of the separation and purification tube, the upper part of the core rod is provided with an air suction hole 9, the piston is fixedly arranged at the top of the core rod, and the negative pressure suction interface is positioned at the bottom of the core rod and extends out of the separation and purification tube. The negative pressure suction interface is communicated with a negative pressure pump (not shown), so that the inside of the separation and purification tube can be pumped into negative pressure, and adipose tissues can be conveniently sucked out of a human body and enter the separation and purification tube along the adipose suction tube and the Ruhr three-way valve for separation, extraction, concentration and purification.
Further, the fat suction pipe comprises a suction pipe body 2 and a suction pipe base 3, a fat suction hole 1 is formed in the upper portion of the suction pipe body, the suction pipe base comprises a connecting pipe 17 and a clamping support 16, the clamping support is clamped at the top of the Ruhr three-way valve, and the connecting pipe fixedly penetrates through the clamping support and is communicated with the suction pipe body and the Ruhr three-way valve. The clamping support is convenient to form stable connection with the Ruhr three-way valve, so that adipose tissues smoothly enter the separation and purification tube through the Ruhr three-way valve, and a fat suction tube is quickly taken down from the Ruhr three-way valve after the suction is finished, and the next separation, extraction, concentration and purification operations are performed. The suction tube base can be integrated into one piece, and preparation simple to operate, and the structure is firm.
Further, separation and purification pipe top is equipped with collects mouth 5, and bottom fixed mounting has shrouding 11, luer three-way valve installs on separation and purification pipe's collection mouth, and simple to operate is swift to prevent that adipose tissue from taking place to leak, the shrouding set up the fixed mounting who makes things convenient for negative pressure generating device.
Further, a pressing hand 12 is fixedly arranged on the core bar above the negative pressure suction interface, and after the suction of the adipose tissues is finished, the pressing hand can be held to push the core bar upwards, so that the piston moves upwards along the filter screen cylinder, and the adipose tissues are pushed into the Ruhr three-way valve to be separated, extracted, concentrated and purified.
Optimally, the Ruhr three-way valve is provided with a Ruhr interface, so that the Ruhr three-way valve is conveniently and quickly connected and disconnected with the fat suction tube, the separation and purification tube and the Ruhr injector.
Optimally, the negative pressure suction interface is a threaded bevel opening, so that the negative pressure suction interface is conveniently and quickly and tightly connected with the negative pressure pump, and the falling or leakage is prevented.
Optimally, the fat suction holes are multiple, so that the fat suction speed can be further increased.
When the separation, extraction, concentration and purification device of the nano fat needs to separate, extract, concentrate and purify the adipose tissue, firstly, a fat suction pipe is installed on a Ruhr three-way valve, the top of the fat suction pipe enters the adipose tissue, the Ruhr three-way valve is in a state that the fat suction pipe is communicated with the separation and purification pipe, then a negative pressure suction interface is communicated with a negative pressure pump, the negative pressure pump works, the separation and purification pipe is pumped into a negative pressure state, and the adipose tissue quickly enters the separation and purification pipe from the fat suction pipe through the Ruhr three-way valve. Then the negative pressure pump stops working, the negative pressure pump is separated from the negative pressure suction interface, the fat suction pipe is taken down from the Ruhr three-way valve, the first Ruhr injector 14 and the second Ruhr injector 15 are respectively arranged on the two Ruhr joints of the Ruhr three-way valve, repeated flushing is carried out through the Ruhr three-way valve, the fat particles are mechanically emulsified, the generated liquid oil drops are filtered and removed through the filter screen cylinder, so that the obtained concentrated and purified nano fat is pumped out from the Ruhr injector, and then the operations of separating, extracting, concentrating and purifying the fat tissue are completed. And the separated waste liquid such as liquid oil drops and the like falls after penetrating through the filter screen cylinder above the piston and enters the filter screen cylinder from the lower part of the piston, enters the core rod from the air suction hole and flows out along the negative pressure suction interface, and a waste liquid collecting bottle (not shown) can be placed at the bottom of the negative pressure suction interface to collect the waste liquid.
In conclusion, the device for separating, extracting, concentrating and purifying the nano fat has the advantages of simple structure, small volume, light weight, convenience and labor saving in operation, can be used for efficiently separating and extracting the nano fat on the basis of keeping the activity of nano fat tissues, improving the biological activity of the nano fat through purification and concentration, and reducing adverse reactions caused by injection of the nano fat, has the advantages of high purity and good activity, and is suitable for application in the medical fields of plastic surgery, joint surgery and the like.
The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (7)
1. A separation of nanometer fat is drawed and is concentrated purification device which characterized in that: the device comprises a fat suction pipe, a separation and purification pipe and a negative pressure suction device, wherein the fat suction pipe is positioned above the separation and purification pipe and is communicated with the separation and purification pipe through a Ruhr three-way valve, a filter screen cylinder is installed in the separation and purification pipe, the negative pressure suction device comprises a core rod, a piston and a negative pressure suction interface, the core rod extends into the filter screen cylinder from the bottom of the separation and purification pipe, a suction hole is formed in the upper part of the core rod, the piston is fixedly installed at the top of the core rod, and the negative pressure suction interface is positioned at the bottom of the core rod and extends out of the separation and purification pipe.
2. The apparatus for separating, extracting, concentrating and purifying nano-fat as claimed in claim 1, wherein the fat suction tube comprises a suction tube body and a suction tube base, the upper portion of the suction tube body is provided with a fat suction hole, the suction tube base comprises a connection tube and a clamping holder, the clamping holder is clamped on the top of the luer three-way valve, and the connection tube fixedly passes through the clamping holder and is communicated with the suction tube body and the luer three-way valve.
3. The apparatus for separating, extracting, concentrating and purifying nano-fat according to claim 1, wherein the separation and purification tube has a collection port at the top and a sealing plate at the bottom, and the luer three-way valve is installed at the collection port of the separation and purification tube.
4. The device for separating, extracting, concentrating and purifying nano-fat according to claim 1, wherein the core bar is fixedly provided with a press hand above the negative pressure suction port.
5. The apparatus for separating, extracting, concentrating and purifying nano-fat according to claim 1, wherein the luer three-way valve is provided with a luer.
6. The apparatus for separating, extracting, concentrating and purifying nano-fat according to claim 1, wherein the negative pressure suction port is a threaded bevel port.
7. The apparatus for separating, extracting, concentrating and purifying nano-fat according to claim 2, wherein the fat suction hole is plural.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN202210599625.9A CN114874883A (en) | 2022-05-30 | 2022-05-30 | Separation of nanometer fat is drawed and concentrated purification device |
Applications Claiming Priority (1)
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CN202210599625.9A CN114874883A (en) | 2022-05-30 | 2022-05-30 | Separation of nanometer fat is drawed and concentrated purification device |
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CN114874883A true CN114874883A (en) | 2022-08-09 |
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CN202210599625.9A Pending CN114874883A (en) | 2022-05-30 | 2022-05-30 | Separation of nanometer fat is drawed and concentrated purification device |
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- 2022-05-30 CN CN202210599625.9A patent/CN114874883A/en active Pending
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