CN114848543A - Natural soothing composition and application thereof in cosmetics - Google Patents
Natural soothing composition and application thereof in cosmetics Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9755—Gymnosperms [Coniferophyta]
- A61K8/9761—Cupressaceae [Cypress family], e.g. juniper or cypress
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9794—Liliopsida [monocotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
- A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The invention relates to the field of cosmetics, and particularly relates to a natural soothing composition and application thereof in cosmetics. The invention discloses a soothing composition which comprises the following components in parts by mass: oat bran extract 2-20 parts, large-leaf gentian root extract 10-50 parts, and selaginella tamariscina extract 1-5 parts. The invention prepares the soothing composition by extracting through a modern plant extraction technology. Cell tests and consumer use tests prove that the soothing composition prepared by the embodiment of the invention is beneficial to improving inflammatory changes caused by skin contact with cosmetics, and can be used as an active additive to be applied to a cosmetic formula to play a role in prevention and soothing.
Description
Technical Field
The invention relates to the field of cosmetics, and particularly relates to a natural soothing composition and application thereof in cosmetics.
Background
According to the cosmetic classification rules and catalogs issued by the drug administration (P4), soothing is defined as: it is effective in improving skin irritation. According to the technical specifications for cosmetic safety (2015 edition) (P490), the definition of skin irritation dermal irration: reversible inflammatory changes that occur locally after application of the test substance to the skin. Therefore, the primary improvement in soothing is reversible inflammatory changes caused by skin contact with foreign substances such as cosmetics.
In recent years, allergic patients have been rapidly increased with changes in dietary structure, air pollution, and increase in life and work pressure, and skin sensitivity has become a social problem affecting human health. According to incomplete statistics, the population of the global self-sensation sensitive skin is more and more, wherein the male accounts for 38% and the female accounts for 61%. Statistical data of five skin disease diagnosis mechanisms 2003 and 2004 determined by the ministry of health in China show that contact dermatitis is the most common of the six major cosmetic skin diseases, and the incidence rates of two years respectively account for 82.6% and 93.3% of the total incidence rate of the cosmetic skin diseases (screening, extraction and mechanism research of anti-allergic and anti-irritant active substances for cosmetics [ D ] Beijing university of Industrial and commercial, 2010.). On the other hand, under the situation that the cosmetic manufacturing industry is continuously developing, the types of raw materials which can be used for manufacturing cosmetics are continuously increasing, and the number of raw materials used for cosmetic formulations is also continuously increasing. Therefore, cosmetic safety risks are increasing.
In order to reduce the safety risk of cosmetic preparation and use as much as possible and make the product milder, various major cosmetic companies add plant extracts, active polypeptides and other skin allergy resistant active ingredients into the formula to play a role in prevention and relief. For example, a french Xuanerya cosmetic is added with chamomile in a formulation to improve the skin immune defense ability, and hamamelis virginiana extract is added to a cosmetic in countries of europe and america to promote the skin allergic dermatitis repair (research progress on an evaluation model of cosmetic anti-sensitivity safety [ J ] daily chemical science, 2021,44(03): 48-54.).
Based on the above, the invention provides a relieving composition with natural sources, which is beneficial to improving inflammatory changes caused by skin contact with cosmetics, and can be used as an active additive to be applied to a cosmetic formula to play a role in prevention and relieving.
Disclosure of Invention
The main object of the present invention is to provide a soothing composition.
The above object of the present invention is achieved by the following technical solutions:
a soothing composition comprises the following components in parts by mass: oat bran extract 2-20 parts, large-leaf gentian root extract 10-50 parts, and selaginella tamariscina extract 1-5 parts.
In a preferred embodiment of the present invention, the soothing composition comprises the following components in parts by weight: 10 parts of oat bran extract, 30 parts of gentiana macrophylla extract and 3 parts of selaginella tamariscina extract.
The oat bran extract, the gentiana macrophylla extract and the selaginella tamariscina extract are matched, so that the oat bran extract, the gentiana macrophylla extract and the selaginella tamariscina extract are beneficial to improving inflammatory changes caused when skin is contacted with cosmetics, can be used as an active additive to be applied to a formula of the cosmetics, and have the effects of preventing and relieving.
In some embodiments of the present invention, the gentiana macrophylla extract comprises a combination of a gentiana macrophylla root extract, a gentiana macrophylla leaf extract and a gentiana macrophylla flower extract.
According to the invention, by compounding the gentiana macrophylla root extract, the gentiana macrophylla leaf extract and the gentiana macrophylla flower extract with the oat bran extract and the selaginella tamariscina extract, the soothing capacity is stronger.
The embodiment of the invention also provides a preparation method of the relieving composition, which comprises the following steps:
(1) respectively weighing the following raw materials in parts by weight and crushing: oat bran 2-20 parts, large-leaf gentian root 5-30 parts, large-leaf gentian leaf 2-10 parts, large-leaf gentian flower 3-10 parts, and selaginella tamariscina herb 1-5 parts;
(2) soaking oat bran in 90% V/V ethanol water solution, wherein the feed-liquid ratio is 1: 8-1: 15, adjusting the pH value to be weakly acidic, and stirring and extracting to obtain an extracting solution A;
(3) adding water into the extracting solution A to reduce the ethanol concentration to 70% V/V to obtain a diluent B; mixing radix Gentianae Marcrophyllae, folium Gentianae Marcrophyllae, flos Gentianae Marcrophyllae, and herba Selaginellae, adding appropriate amount of diluent B, soaking overnight, and transferring into percolation tank; eluting with the rest diluent B until the eluate is drained to obtain eluate, and concentrating by rotary evaporation to remove ethanol to obtain concentrated solution;
(4) loading the concentrated solution onto AB-8 macroporous resin column, eluting with 80% V/V ethanol water solution, collecting eluate 4-5BV, and concentrating by rotary evaporation to dry to obtain powder.
In some of these embodiments, the pH in step (2) is 4-5; the temperature for stirring and extracting is 55-65 deg.C, and the extraction time is 3-5 hr.
In some of these embodiments, the elution in step (3) is performed at a flow rate of 1-2 BV/h.
In some of these embodiments, the elution in step (4) is performed at a flow rate of 1-2 BV/h.
The technical scheme provided by the embodiment of the invention has the following beneficial effects: the invention selects Gentiana macrophylla root, Gentiana macrophylla leaf, Gentiana macrophylla flower, Selaginella tamariscina and oat bran, and extracts the root, the leaves, the flowers, the Selaginella tamariscina and the oat bran by a modern plant extraction technology to prepare the soothing composition. Cell tests and consumer use tests prove that the soothing composition prepared by the embodiment of the invention is beneficial to improving inflammatory changes caused by skin contact with cosmetics, and can be used as an active additive to be applied to a cosmetic formula to play a role in prevention and soothing.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art are briefly introduced below.
FIG. 1 comparison of the change before and after volunteers applied a soothing mask.
Detailed Description
The present invention will now be described in detail with reference to specific examples, but the scope of the present invention is not limited thereto.
Materials, reagents and the like used in examples of the present invention are commercially available unless otherwise specified.
The oat bran related by the invention is the part of oat containing most dietary fiber, especially contains the most abundant beta-glucan which belongs to soluble dietary fiber, is a known blood fat reducing effective component and is beneficial to intestinal health, so the oat fiber is known as noble dietary fiber.
The gentiana macrophylla related by the invention is a gentiana plant of gentianaceae and gentiana. Perennial herbs with a height of 30-60 cm, the whole plant is smooth and hairless, and the basal part is wrapped by withered fibrous leaf sheath. The fibrous root is multiple, twisted or bonded into a cylindrical root. Branches are tufted, either upright or ascending in an oblique manner. The rosette leaves are oval or narrow oval, 6-28 cm long, 2.5-6 cm wide, blunt or sharp at the tip, and gradually narrowed at the base. Most flowers have no pedicel, and the tops of clustered branches are in a head shape or are in a wheel shape when axillary growing; the calyx shaped tube membrane is yellow green or sometimes purple, and has a length of (3)7-9 mm, and one side of the calyx shaped tube membrane is cracked to be in a shape of spatulate Bodinieri, and the tip of the calyx shaped tube membrane is truncated or circular. Gentiana macrophylla is bitter and mild in nature and slightly cold, enters stomach, liver and gallbladder channels, is used as a medicine by root, has the effects of dispelling wind and removing dampness, promoting blood circulation and relaxing channels, clearing heat and promoting urination, and is used for treating diseases such as rheumatic arthralgia, spasm of muscles and tendons, hectic fever due to yin-deficiency and damp-heat jaundice and the like.
The tamariskoid spikemoss herb is a revived plant of the genus tamariskoid spikemoss of the family tamariskoid spikemoss, and is in a cushion shape. Reproduction by spores. The root of the tamariskoid spikemoss herb, namely the nine dead hempleaf groundsel herb, can be automatically separated from the soil, curls like a fist, moves along with the wind, becomes honored when meeting water, and then drills into the soil again to find the water. Because of the strong drought resistance, the root system can be stretched after being soaked in water after long-term drought, so the drought-resistant plant is named. It is not only ornamental, but also medicinal, and the whole herb has the efficacy of hemostasis and convergence. The whole plant is burnt into ash in folk, and the powder can be taken orally to treat various hemorrhagic diseases, and can be mixed with vegetable oil for external use to treat various wounds.
Example 1
A soothing composition is prepared by extracting:
(1) respectively weighing the following raw materials in parts by weight and crushing: oat bran 2 parts, large-leaf gentian root 30 parts, large-leaf gentian leaf 10 parts, large-leaf gentian flower 10 parts, and selaginella tamariscina herb 1 part;
(2) soaking oat bran in 90% V/V ethanol water solution, wherein the feed-liquid ratio is 1: 15, adding hydrochloric acid to adjust the pH value to 4, and stirring and extracting for 3 hours at 65 ℃ to obtain an extracting solution A;
(3) adding water into the extracting solution A to reduce the ethanol concentration to 70% V/V to obtain a diluent B; mixing radix Gentianae Marcrophyllae, folium Gentianae Marcrophyllae, flos Gentianae Marcrophyllae, and herba Selaginellae, adding appropriate amount of diluent B, soaking overnight with the liquid level just 1-2cm higher than the original material level, and transferring into percolation tank; eluting with the rest diluent B until the diluent is drained, wherein the flow rate is 2BV/h, obtaining an eluent, and performing rotary evaporation and concentration to remove ethanol, thereby obtaining a concentrated solution;
(4) loading the concentrated solution onto AB-8 macroporous resin column, eluting with 80% V/V ethanol water solution at flow rate of 2BV/h, collecting eluate 4BV, and concentrating by rotary evaporation to dry to obtain powder.
Example 2
A soothing composition is prepared by extracting:
(1) respectively weighing the following raw materials in parts by weight and crushing: 20 parts of oat bran, 5 parts of gentiana macrophylla root, 2 parts of gentiana macrophylla leaf, 3 parts of gentiana macrophylla flower and 5 parts of selaginella tamariscina whole plant;
(2) soaking oat bran in 90% V/V ethanol water solution, wherein the feed-liquid ratio is 1: adding citric acid to adjust the pH value to 5, and stirring and extracting for 5 hours at 55 ℃ to obtain an extracting solution A;
(3) adding water into the extracting solution A to reduce the ethanol concentration to 70% V/V to obtain a diluent B; mixing radix Gentianae Marcrophyllae, folium Gentianae Marcrophyllae, flos Gentianae Marcrophyllae, and herba Selaginellae, adding appropriate amount of diluent B, soaking overnight with the liquid level just 1-2cm higher than the original material level, and transferring into percolation tank; eluting with the rest diluent B until the diluent is drained, wherein the flow rate is 1BV/h, obtaining an eluent, and performing rotary evaporation and concentration to remove ethanol, thereby obtaining a concentrated solution;
(4) loading the concentrated solution on an AB-8 macroporous resin column, eluting with 80% V/V ethanol water solution at the flow rate of 1BV/h, collecting the eluent of 5BV, and concentrating by rotary evaporation to dryness to obtain powder.
Example 3
A soothing composition is prepared by extracting:
(1) respectively weighing the following raw materials in parts by weight and crushing: 10 parts of oat bran, 20 parts of gentiana macrophylla root, 5 parts of gentiana macrophylla leaf, 5 parts of gentiana macrophylla flower and 3 parts of selaginella tamariscina whole plant;
(2) soaking oat bran in 90% V/V ethanol water solution, wherein the feed-liquid ratio is 1: 12, adding hydrochloric acid to adjust the pH value to 4.5, and stirring and extracting for 4 hours at 60 ℃ to obtain an extracting solution A;
(3) adding water into the extracting solution A to reduce the ethanol concentration to 70% V/V to obtain a diluent B; mixing radix Gentianae Marcrophyllae, folium Gentianae Marcrophyllae, flos Gentianae Marcrophyllae, and herba Selaginellae, adding appropriate amount of diluent B, soaking overnight with the liquid level just 1-2cm higher than the original material level, and transferring into percolation tank; eluting with the rest diluent B until the diluent is drained, wherein the flow rate is 1.5BV/h, obtaining an eluent, and performing rotary evaporation and concentration to remove ethanol, thereby obtaining a concentrated solution;
(4) loading the concentrated solution on AB-8 macroporous resin column, eluting with 80% V/V ethanol water solution at flow rate of 1.5BV/h, collecting eluate at 4.5BV, and concentrating by rotary evaporation to dry to obtain powder.
Comparative example 1
A soothing composition is prepared by extracting the following steps:
(1) respectively weighing the following raw materials in parts by weight and crushing: 10 parts of oat bran, 20 parts of gentiana macrophylla root, 5 parts of gentiana macrophylla leaf, 5 parts of gentiana macrophylla flower and 3 parts of selaginella tamariscina whole plant;
(2) mixing oat bran, roots of gentiana macrophylla, leaves of gentiana macrophylla, flowers of gentiana macrophylla and whole grass of selaginella tamariscina, soaking in 90% V/V ethanol water solution, wherein the material-liquid ratio is 1: 12, adding hydrochloric acid to adjust the pH value to 4.5, and stirring and extracting for 4 hours at 60 ℃ to obtain an extracting solution A; performing rotary evaporation concentration to remove ethanol to obtain a concentrated solution;
(3) loading the concentrated solution on AB-8 macroporous resin column, eluting with 80% V/V ethanol water solution at flow rate of 1.5BV/h, collecting eluate at 4.5BV, and concentrating by rotary evaporation to dry to obtain powder.
Comparative example 2
A soothing composition is prepared by extracting:
(1) respectively weighing the following raw materials in parts by weight and crushing: 10 parts of oat bran, 20 parts of gentiana macrophylla root, 5 parts of gentiana macrophylla leaf, 5 parts of gentiana macrophylla flower and 3 parts of selaginella tamariscina whole plant;
(2) mixing oat bran, radix Gentianae Marcrophyllae leaf, flos Gentianae Marcrophyllae, and herba Selaginellae, adding 70% V/V ethanol water solution, soaking overnight with the liquid level just 1-2cm higher than the raw material level, and transferring into a percolation tank; eluting with 70% V/V ethanol water solution at flow rate of 1.5BV/h to obtain 2BV eluate, and concentrating by rotary evaporation to remove ethanol to obtain concentrated solution;
(3) loading the concentrated solution on AB-8 macroporous resin column, eluting with 80% V/V ethanol water solution at flow rate of 1.5BV/h, collecting eluate at 4.5BV, and concentrating by rotary evaporation to dry to obtain powder.
Comparative example 3
A soothing composition is prepared by extracting:
(1) respectively weighing the following raw materials in parts by weight and crushing: 10 parts of oat bran;
(2) soaking oat bran in 90% V/V ethanol water solution, wherein the feed-liquid ratio is 1: 12, adding hydrochloric acid to adjust the pH value to 4.5, and stirring and extracting for 4 hours at 60 ℃ to obtain an extracting solution A;
(3) carrying out rotary evaporation and concentration on the extracting solution A to remove ethanol, thus obtaining a concentrated solution;
(4) loading the concentrated solution on AB-8 macroporous resin column, eluting with 80% V/V ethanol water solution at flow rate of 1.5BV/h, collecting eluate at 4.5BV, and concentrating by rotary evaporation to dry to obtain powder.
Comparative example 4
A soothing composition is prepared by extracting the following steps:
(1) respectively weighing the following raw materials in parts by weight and crushing: 20 parts of gentiana macrophylla root, 5 parts of gentiana macrophylla leaf and 5 parts of gentiana macrophylla flower;
(2) mixing radix Gentianae Marcrophyllae, folium Gentianae Marcrophyllae and flos Gentianae Marcrophyllae, adding 70% V/V ethanol water solution, soaking overnight with the liquid level just 1-2cm higher than the original material level, and transferring into percolation tank; eluting with 70% V/V ethanol water solution at flow rate of 1.5BV/h to obtain 2BV eluate, and concentrating by rotary evaporation to remove ethanol to obtain concentrated solution;
(3) loading the concentrated solution on AB-8 macroporous resin column, eluting with 80% V/V ethanol water solution at flow rate of 1.5BV/h, collecting eluate at 4.5BV, and concentrating by rotary evaporation to dry to obtain powder.
Comparative example 5
A soothing composition is prepared by extracting:
(1) respectively weighing the following raw materials in parts by weight and crushing: 3 parts of selaginella tamariscina;
(2) adding herba Selaginellae into 70% V/V ethanol water solution, soaking overnight when the liquid level is 1-2cm higher than the original material level, and transferring into percolation tank; eluting with 70% V/V ethanol water solution at flow rate of 1.5BV/h to obtain 2BV eluate, and concentrating by rotary evaporation to remove ethanol to obtain concentrated solution;
(3) loading the concentrated solution on AB-8 macroporous resin column, eluting with 80% V/V ethanol water solution at flow rate of 1.5BV/h, collecting eluate at 4.5BV, and concentrating by rotary evaporation to dry to obtain powder.
Test 1 in vitro method for soothing efficacy
The soothing compositions prepared in examples 1-3 and comparative examples 1-5 were tested for efficacy by in vitro methods, specifically with reference to the group standard TSHRH033-2020 cosmetic soothing efficacy test in vitro-TNF-alpha inflammatory factor assay.
TNF- α is an inflammatory cytokine found in the tumor inflammatory microenvironment, primarily secreted by monocytes/macrophages, that initiates chronic inflammatory responses. TNF-alpha regulates inflammatory response by activating two inflammatory signal pathways of nuclear transcription factor kB (NF-kB) and activated protein 1(AP-1), is an important inflammatory factor and participates in pathological injury of certain autoimmune diseases.
Bacterial Lipopolysaccharide (LPS) is combined with a macrophage surface antigen recognition receptor to induce macrophages and secrete various cytokines such as TNF-alpha and the like. TNF-alpha can activate three signal paths of Caspase protease, JNK and transcription factor NF-kB, realize biological functions of cytotoxicity, antivirus, immunoregulation, apoptosis and the like, and is one of important inflammatory factors. LPS induces RAW264.7, is a classical cell model for researching inflammatory factors, and evaluates the effect of the test object in inhibiting the secretion of TNF-alpha by comparing the difference of the content of TNF-alpha secreted by RAW264.7 after the negative control and the test object are administrated. The TNF-alpha content is measured by an enzyme-linked immunosorbent assay (ELISA) according to the following specific principle: after the TNF-alpha is specifically combined with the TNF-alpha antibody coated on the enzyme label plate, the TNF-alpha is combined with the anti-TNF-alpha antibody with a substrate label, a colored product is generated after the substrate is catalyzed by enzyme, and the content of the TNF-alpha is in positive correlation with the color depth of the colored product. And measuring an Optical Density (OD) value by using a microplate reader at the wavelength of 450nm, and calculating the content of the TNF-alpha. The rate of TNF-. alpha.inhibition was then calculated according to the following formula.
The inhibition rate (%) - (1-T/C) × 100%, wherein T is the average content of TNF-alpha in the test substance, and C is the average content of TNF-alpha in the negative control under the action of LPS.
The specific test results are shown in table 1.
TABLE 1 TNF-d inhibition for each test sample
| Test sample | Test concentration | Inhibition rate |
| Dexamethasone | 100μg/mL | 29.53％ |
| Example 1 | 100μg/mL | 21.13％ |
| Example 2 | 100μg/mL | 24.80％ |
| Example 3 | 100μg/mL | 20.56％ |
| Comparative example 1 | 100μg/mL | 10.39％ |
| Comparative example 2 | 100μg/mL | 14.42％ |
| Comparative example 3 | 100μg/mL | 3.94％ |
| Comparative example 4 | 100μg/mL | 8.16％ |
| Comparative example 5 | 100μg/mL | 4.42％ |
As can be seen from Table 1, the soothing compositions prepared in the examples of the present invention have a good effect of inhibiting the content of TNF- α.
Example 4
The soothing mask comprises the following components in percentage by mass: 3% of glycerin, 2% of butanediol, 0.5% of betaine, 0.05% of trehalose, 0.05% of xanthan gum, 0.15% of sodium hyaluronate, 0.05% of hydroxyethyl cellulose, 1% of the soothing composition prepared in example 2, 0.5% of hexanediol, 0.5% of p-hydroxyacetophenone, 0.001% of essence and the balance of water.
The preparation method comprises the following steps: putting water and hydroxyethyl cellulose into an emulsifying pot, heating to 85 ℃ under stirring, and homogenizing for 1-3 minutes; uniformly wetting glycerol and butanediol with xanthan gum and sodium hyaluronate in advance, and then putting into an emulsifying pot; putting betaine, trehalose, hexanediol and p-hydroxyacetophenone into an emulsifying pot; starting homogenizing for 1-3 min; stirring at 85 deg.C for 30 min, slowly cooling to 45 deg.C, adding the soothing composition and essence, stirring, and discharging to obtain semi-finished product. And weighing 25g of the semi-finished product, filling the semi-finished product into a mask bag containing mask cloth, and carrying out heat sealing to obtain the allergy-relieving mask.
Test 2 trial test in volunteers
31 volunteers were called out and the soothing mask prepared in example 4 was subjected to a volunteer trial test.
And (3) inclusion standard: the face is red, itching, stabbing and the like.
The using method comprises the following steps: after the face is cleaned by clear water, the mask is covered on the face for 15-30 minutes, the mask is slightly removed, and then the mask is cleaned by clear water, the mask is respectively used for 1 time in the morning and at night, and the observation results are obtained after the mask is continuously used for 1 month, and the statistics are shown in the following table 2.
The criteria for efficacy evaluation are as follows:
the effect is shown: the face is reddish, the itch and the stabbing pain disappear completely;
the method has the following advantages: redness, itching, reduced stinging of the face;
and (4) invalidation: the face was reddened, itchy, and tingling had no detail or was more severe.
TABLE 2 volunteer trial test record
| Test sample | Show effect | Is effective | Nullification | Effective rate% |
| Soothing mask | 11 | 18 | 2 | 93.54 |
As can be seen from table 2, the soothing mask prepared in the examples of the present invention produced good soothing effect.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (7)
1. The soothing composition is characterized by comprising the following components in parts by mass: oat bran extract 2-20 parts, large-leaf gentian root extract 10-50 parts, and selaginella tamariscina extract 1-5 parts.
2. A soothing composition according to claim 1, wherein the soothing composition comprises the following components in parts by weight: 10 parts of oat bran extract, 30 parts of gentiana macrophylla extract and 3 parts of selaginella tamariscina extract.
3. The soothing composition of claim 1, wherein the extract of gentiana macrophylla comprises a combination of extracts of roots, leaves and flowers of gentiana macrophylla.
4. A soothing composition according to any of claims 1-3, wherein the soothing composition is prepared by a process comprising the steps of:
(1) respectively weighing the following raw materials in parts by weight and crushing: oat bran 2-20 parts, large-leaf gentian root 5-30 parts, large-leaf gentian leaf 2-10 parts, large-leaf gentian flower 3-10 parts, and selaginella tamariscina herb 1-5 parts;
(2) soaking oat bran in 90% V/V ethanol water solution, wherein the feed-liquid ratio is 1: 8-1: 15, adjusting the pH value to be weakly acidic, and stirring and extracting to obtain an extracting solution A;
(3) adding water into the extracting solution A to reduce the ethanol concentration to 70% V/V to obtain a diluent B; mixing radix Gentianae Marcrophyllae, folium Gentianae Marcrophyllae, flos Gentianae Marcrophyllae, and herba Selaginellae, adding appropriate amount of diluent B, soaking overnight, and transferring into percolation tank; eluting with the rest diluent B until the eluate is drained to obtain eluate, and concentrating by rotary evaporation to remove ethanol to obtain concentrated solution;
(4) loading the concentrated solution onto AB-8 macroporous resin column, eluting with 80% V/V ethanol water solution, collecting eluate 4-5BV, and concentrating by rotary evaporation to dry to obtain powder.
5. A soothing composition according to claim 4, wherein in step (2) the pH is in the range of 4 to 5; the temperature for stirring and extracting is 55-65 deg.C, and the extraction time is 3-5 hr.
6. A soothing composition according to claim 4, wherein the elution in step (3) is at a flow rate of 1-2 BV/h.
7. A soothing composition according to claim 4, wherein the elution in step (4) is at a flow rate of 1-2 BV/h.
Priority Applications (1)
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106333914A (en) * | 2016-08-24 | 2017-01-18 | 广东芭薇生物科技股份有限公司 | Quickly-absorbed moisturizing mask and preparation method thereof |
| CN112274461A (en) * | 2020-11-09 | 2021-01-29 | 云南英格生物技术有限公司 | Gentiana macrophylla extract and preparation method and application thereof |
| CN112426512A (en) * | 2020-12-30 | 2021-03-02 | 蒋位宏 | Massage liquid for relieving neck, shoulder, waist and leg and preparation method thereof |
| CN114146030A (en) * | 2020-09-08 | 2022-03-08 | 广州市普高管理咨询有限公司 | Itching-relieving and soothing shower gel and preparation method thereof |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106333914A (en) * | 2016-08-24 | 2017-01-18 | 广东芭薇生物科技股份有限公司 | Quickly-absorbed moisturizing mask and preparation method thereof |
| CN114146030A (en) * | 2020-09-08 | 2022-03-08 | 广州市普高管理咨询有限公司 | Itching-relieving and soothing shower gel and preparation method thereof |
| CN112274461A (en) * | 2020-11-09 | 2021-01-29 | 云南英格生物技术有限公司 | Gentiana macrophylla extract and preparation method and application thereof |
| CN112426512A (en) * | 2020-12-30 | 2021-03-02 | 蒋位宏 | Massage liquid for relieving neck, shoulder, waist and leg and preparation method thereof |
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