CN114831226A - Compound probiotic-containing nutrient special for feeding chickens and preparation method thereof - Google Patents
Compound probiotic-containing nutrient special for feeding chickens and preparation method thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
- A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/26—Animal feeding-stuffs from material of animal origin from waste material, e.g. feathers, bones or skin
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/24—Compounds of alkaline earth metals, e.g. magnesium
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/30—Oligoelements
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K40/00—Shaping or working-up of animal feeding-stuffs
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
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- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The nutrient is composed of phyllostachys praecox powder, pennisetum hydridum powder, immortal grass powder, a traditional Chinese medicine preparation, compound vitamins and compound probiotic fermentation liquor, and can promote the absorption of calcium, manganese and iron by chicken organisms through a microbial fermentation technology, can generate B vitamins, vitamin K, amylase and other substances in chicken intestinal tracts to have a health-care effect, convert trace elements of calcium and manganese into calcium lactate, calcium citrate, manganese lactate, manganese alginate and the like which are easy to absorb, and enhance the physique of breeding hens; the leaf eating grass, the grain bamboo grass and the mesona chinensis benth are used as main components in the nutrient, are rich in various amino acids, vitamins, mineral substances, small molecular structures and other nutrient components, have the effects of promoting the metabolism of an organism and improving the immunity of the organism, are rich in various nutrient elements beneficial to a breeding hen organism, have the protein content of more than 40 percent, and are beneficial to increasing the appetite of poultry and livestock, strengthening the physique and growing; the invention has simple process and safe and reliable implementation.
Description
Technical Field
The invention relates to the technical field of poultry breeding, in particular to a special nutrient containing composite probiotics for feeding chickens and a preparation method thereof.
Background
The existing chicken feed has low absorptivity for manganese and calcium, and most of the feeds mainly comprise corns, so that the chickens are easy to lack manganese and calcium, and are easy to lose weight, have prolapsed wings, swollen knee joints, wear, some muscle ulcerations, touch muscle bodies slipping, short and thick legs and bones, deform into flat or twisted, cannot stand, and some toes shrink inwards. The chicken is not easy to eat in daily life, and most of protein in the feed uses substances such as soybeans, peanuts, rapes, cotton seed cakes and cake bran, so that the materials are relatively lack and the price is higher; the fish meal is used, the price of the meat meal is high, and the meat meal is difficult to be fully supplied.
Therefore, a special nutrient containing composite probiotics for feeding chickens, which has high protein content, is easy to absorb, can supplement various trace elements and is low in price, and a preparation method thereof are needed.
Disclosure of Invention
The invention aims to solve the technical problems that the existing feed is low in absorption efficiency, difficult to expect in effect and single in component, and cannot supplement various trace elements, particularly manganese and calcium, and provides the special nutrient containing the composite probiotics for the raised chickens, which is high in protein, easy to absorb and capable of supplementing various trace elements, particularly manganese and calcium.
The invention also aims to provide a preparation method of the special nutrient containing the composite probiotics for the raised chickens.
In order to achieve the first object, the invention adopts the technical scheme that:
a special nutrient containing composite probiotics for raising chickens comprises the following components in parts by weight: 150-180 parts of lysimachia powder, 50-80 parts of pennisetum hydridum powder, 40-50 parts of immortal grass powder, 8-10 parts of Chinese medicinal preparation, 6-8 parts of compound vitamin and 10-30 parts of compound probiotic fermentation broth; the composite probiotic fermentation liquor is prepared by carrying out liquid submerged fermentation on clostridium butyricum and bifidobacterium lactis.
The special nutrient containing the composite probiotics for the raised chickens comprises 9.5-18.5% of trace element manganese in clostridium butyricum fermentation liquor; the bifidobacterium lactis fermentation liquor contains 5.5-14.0% of trace element calcium.
The ratio of the clostridium butyricum fermentation liquor to the bifidobacterium lactis fermentation liquor is 1: 1.
The special nutrient containing the composite probiotics for the raised chickens comprises the following components in percentage by weight: 3-5% of vitamin A, 2-3% of vitamin D, 10-12% of vitamin E and 2-3% of vitamin K 3 3-5% of vitamin B 2 2-6% of carotene and the balance of rice bran meal.
The special nutrient containing the composite probiotics for the raised chickens is characterized by comprising the following components in parts by weight: 15 parts of kudzu root, 20 parts of coix seed, 15 parts of Chinese yam, 12 parts of fleece-flower root, 5 parts of rhizoma anemarrhenae, 5 parts of liquorice, 5 parts of donkey-hide gelatin, 9 parts of white paeony root, 15 parts of phaseolus calcaratus and 6 parts of hawthorn.
In order to achieve the second object, the invention adopts the technical scheme that:
the preparation method of the special nutrient containing the composite probiotics for the raised chickens is characterized by comprising the following steps:
step 1: preparing a composite probiotic fermentation liquor A for later use;
and 2, step: weighing radix Puerariae, Coicis semen, rhizoma Dioscoreae, Polygoni Multiflori radix, rhizoma anemarrhenae, Glycyrrhrizae radix, colla Corii Asini, radix Paeoniae alba, semen Phaseoli and fructus crataegi according to weight parts, drying, pulverizing, sieving, and mixing to obtain Chinese medicinal preparation B;
and step 3: proportionally mixing vitamin A, vitamin D, vitamin E and vitamin K 3 Vitamin B 2 Adding appropriate amount of water into carotene and rice bran meal, mixing, and baking at 50-60 deg.C to semi-dry to obtain paste mixture C;
and 4, step 4: weighing the leaf eating grass powder, the pennisetum hydridum powder, the immortal grass powder, the traditional Chinese medicine preparation B, the paste mixture C and the composite probiotic fermentation liquor A according to the weight parts, adding a proper amount of water, and uniformly stirring at room temperature to obtain a finished product.
The preparation method of the special nutrient containing the composite probiotics for the raised chickens is characterized in that the preparation method of the clostridium butyricum fermentation liquor in the step 1 comprises the following steps:
step 101: placing Clostridium butyricum in culture medium A containing 1% manganese sulfate and sterilized at 115 deg.C for 30min, and standing at 35-40 deg.C for 12-16h to obtain fermentation strain;
step 102: aseptically transferring the fermentation strain to a culture medium A containing 1% manganese sulfate and sterilized at 115 ℃ for 30min, cooling, stirring and fermenting at a stirring speed of 15.0-25.0r/min, controlling the pH value to be 7.0 +/-0.2 by adding alkali liquor, and culturing for 12-14h to obtain a primary fermentation strain;
step 103: aseptically transferring the first-stage fermentation strain to a culture medium A containing 6.0-12.0% of composite manganese source and sterilized at 115 deg.C for 30min, standing and fermenting at 37 deg.C for 24 hr until the residual sugar content is less than or equal to 0.3-0.7%.
The preparation method of the special nutrient containing the composite probiotics for the raised chickens is characterized in that the culture medium A is an MRS culture medium and comprises the following components in percentage by weight: 2.0-5.0% of beef extract, 2.12-4.17% of peptone, 0.5-1.5% of yeast powder, 0.5-1.5% of wheat root powder, 0.01-0.1% of calcium carbonate, 0.025-0.05% of magnesium sulfate, 0.025-0.05% of manganese sulfate and 6.0-12.0% of composite manganese source, wherein the composite manganese source is one or more of manganese sulfate and manganese alginate.
The preparation method of the special nutrient containing the composite probiotics for the raised chickens is characterized in that the preparation method of the bifidobacterium lactis fermentation liquor in the step 1 comprises the following steps:
step 104: inoculating Bifidobacterium lactis to culture medium B containing 1% calcium carbonate in 5L fermenter sterilized at 115 deg.C for 30min and cooled, stirring at 80r/min for fermentation, adding 25% ammonia water at 37 deg.C to control pH to 5.9 + -0.1, and fermenting at high density for 6.3-7.5 h.
The preparation method of the special nutrient containing the composite probiotics for the raised chickens is characterized in that the culture medium B comprises the following components in percentage by weight: lactose 3.5%, soybean peptone 1.31%, yeast peptone 0.88%, yeast powder 1.31%, and Na 2 HPO 4 0.11% of NaH 2 PO 4 0.3 percent of sodium citrate, 0.43 percent of MgSO 4 0.04% of MnSO 4 0.012 percent, 0.1 percent of Tween-80, 0.05 percent of L-cysteine hydrochloride and 6 to 12 percent of composite inorganic calcium source, wherein the composite inorganic calcium source is one or a plurality of combinations of calcium carbonate and calcium sulfate.
Compared with the prior art, the invention has the following advantages:
1. the application provides a special nutrient containing composite probiotics for feeding chickens, which comprises phyllostachys praecox powder, pennisetum hydridum powder, immortal grass powder, a traditional Chinese medicine preparation, composite vitamins and composite probiotic fermentation liquor, can promote the absorption of calcium, manganese and iron and can generate substances such as B vitamins, vitamin K, amylase and the like in intestinal tracts of chickens through liquid submerged fermentation by adopting clostridium butyricum and bifidobacterium lactis, has a health-care effect, converts trace elements such as calcium lactate, calcium citrate, manganese lactate, manganese alginate and the like which are easy to absorb into calcium lactate, calcium citrate, manganese lactate, manganese alginate and the like, enhances the physique of breeding hens, prevents the diseases such as wing droop, knee joint swelling, abrasion, leg bone short deformation into flat or distortion and incapability of standing and the like caused by lack of manganese and calcium elements, and uses the clostridium butyricum and bifidobacterium lactis fermentation liquor as a special nutrient additive for feeding chickens, and has the function of reducing abuse of antibiotic products in current feeds, Reduce the residue of the drug in the meat, reduce the drug resistance of animal bacteria and ensure the animalHas important significance in health; the leaf eating grass powder is used as the main protein component in the feed, the protein content is more than 30 percent, and the feed is rich in various amino acids, vitamins, mineral substances (potassium, selenium, zinc, iron, calcium) and other nutrient components, and has the effects of promoting the metabolism of the organism and improving the immunity of the organism; the dry matter of the whole plant contains lysine, aspartic acid, glutamic acid, serine, histidine, glycine, threonine, alanine, arginine, tyrosine, valine, methionine, phenylalanine, isoleucine, leucine, vitamin C, vitamin B and the like 1 Vitamin B 2 Lutein, beta-carotene, iron, manganese, zinc and copper with protein content more than 10%, is helpful for improving appetite of livestock and poultry, strengthening physique and growth, and has low cost which can be accepted by most animal husbandry practitioners.
2. The immunity of chicks can be obviously improved by adding medicinal and edible Chinese herbal medicines to prepare the traditional Chinese medicine preparation, the occurrence of diseases of a digestive system can be prevented, and the morbidity of chickens can be greatly reduced, wherein the fleece-flower root has the effects of tonifying essence and blood and strengthening bones and tonifying liver and kidney, the liquorice has the effects of tonifying spleen and replenishing qi and clearing away heat and toxic materials, and a small amount of the traditional Chinese medicine preparation is used as a feed additive, so that the micro-ecological environment of chicken organisms can be improved, the disease resistance of the chickens can be improved, the metabolism of the chickens can be promoted, and high-quality chickens with high nutritional value and low cholesterol can be obtained.
3. The special nutrient containing the composite probiotics for the raised chickens, which is obtained by the preparation method, is simple in production process, safe in construction and good in application prospect.
Detailed Description
The following describes a specific embodiment of the present invention with reference to specific examples 1 to 4 and comparative examples:
example 1:
a preparation method of special nutrient containing composite probiotics for feeding chickens comprises the following steps:
step 101: preparing a culture medium A according to 2.13% of beef extract, 3.27% of peptone, 0.50% of yeast powder, 0.83% of wheat root powder, 0.03% of calcium carbonate, 0.025% of magnesium sulfate, 0.025% of manganese sulfate and 12.0% of a composite manganese source, placing clostridium butyricum in the culture medium A containing 1% of manganese sulfate and sterilized at 115 ℃ for 30min, and standing and culturing for 14h in an environment with the temperature of 37 ℃ to obtain a fermentation strain;
step 102: aseptically transferring the fermentation strain to a culture medium A containing 1% manganese sulfate and sterilized at 115 ℃ for 30min, cooling, stirring and fermenting at a stirring speed of 25.0r/min, controlling the pH value to be 7.0 +/-0.2 by feeding alkali liquor, and culturing for 12h to obtain a primary fermentation strain;
step 103: aseptically transferring the primary fermentation strain to a culture medium A containing a composite manganese source and sterilized at 115 ℃ for 30min, standing and fermenting at 37 ℃ for 24h, and obtaining clostridium butyricum fermentation liquor when the residual sugar content is less than or equal to 0.3-0.7%;
step 104: 3.5% of lactose, 1.31% of soybean peptone, 0.88% of yeast peptone, 1.31% of yeast powder and 0.11% of Na 2 HPO 4 0.3% of NaH 2 PO 4 0.43 percent of sodium citrate, 0.04 percent of MgSO 4 0.012% of MnSO 4 Preparing a culture medium B from 0.1% of tween-80, 0.05% of L-cysteine hydrochloride and 12.00% of compound inorganic calcium source, aseptically transferring the bifidobacterium lactis to the culture medium B containing 1% of calcium carbonate in a 5L fermentation tank which is sterilized at 115 ℃ for 30min and cooled, stirring and fermenting at a stirring speed of 80r/min, controlling the pH value to be between 5.9 +/-0.1 by feeding 25% of ammonia water under the environment of 37 ℃, and culturing for 6.3h by high-density fermentation to obtain bifidobacterium lactis fermentation liquor;
step 2: weighing 15 parts of kudzuvine root, 20 parts of coix seed, 15 parts of Chinese yam, 12 parts of fleece-flower root, 5 parts of common anemarrhena rhizome, 5 parts of liquoric root, 5 parts of donkey-hide gelatin, 9 parts of white paeony root, 15 parts of red bean and 6 parts of hawthorn fruit according to the parts by weight, drying, crushing, sieving and fully mixing to obtain a traditional Chinese medicine preparation B;
and step 3: proportionally mixing 3% of vitamin A, 3% of vitamin D, 10% of vitamin E and 2.5% of vitamin K 3 Account for,3.6% of vitamin B 2 Adding a proper amount of water into 4% of carotene and the balance of rice bran meal, fully mixing, and drying to be semi-dry at 50 ℃ to form a paste mixture C;
and 4, step 4: weighing the leaf eating grass powder, the pennisetum hydridum powder, the immortal grass powder, the traditional Chinese medicine preparation B, the paste mixture C and the composite probiotic fermentation broth A according to the weight parts in the table 1, adding a proper amount of water, and uniformly stirring at room temperature to obtain a finished product.
Example 2:
a preparation method of special nutrient containing composite probiotics for feeding chickens comprises the following steps:
step 101: preparing a culture medium A according to 2.13% of beef extract, 3.27% of peptone, 0.50% of yeast powder, 0.83% of wheat root powder, 0.03% of calcium carbonate, 0.025% of magnesium sulfate, 0.025% of manganese sulfate and 12.0% of a composite manganese source, placing clostridium butyricum in the culture medium A containing 1% of manganese sulfate and sterilized at 115 ℃ for 30min, and standing and culturing for 14h in an environment with the temperature of 37 ℃ to obtain a fermentation strain;
step 102: aseptically transferring the fermentation strain to a culture medium A containing 1% manganese sulfate and sterilized at 115 ℃ for 30min, cooling, stirring and fermenting at a stirring speed of 25.0r/min, controlling the pH value to be 7.0 +/-0.2 by feeding alkali liquor, and culturing for 12h to obtain a primary fermentation strain;
step 103: aseptically transferring the primary fermentation strain to a culture medium A containing a composite manganese source and sterilized at 115 ℃ for 30min, standing and fermenting at 37 ℃ for 24h, and obtaining clostridium butyricum fermentation liquor when the residual sugar content is less than or equal to 0.3-0.7%;
step 104: 3.5% of lactose, 1.31% of soybean peptone, 0.88% of yeast peptone, 1.31% of yeast powder and 0.11% of Na 2 HPO 4 0.3% of NaH 2 PO 4 0.43 percent of sodium citrate, 0.04 percent of MgSO 4 0.012% of MnSO 4 Preparing a culture medium B from 0.1% of tween-80, 0.05% of L-cysteine hydrochloride and 12.00% of compound inorganic calcium source, aseptically transferring Bifidobacterium lactis to the culture medium B containing 1% of calcium carbonate in a 5L fermentation tank sterilized at 115 ℃ for 30min and cooled, and stirring at a stirring speed of 80r/minFermenting, namely feeding 25% ammonia water at 37 ℃ to control the pH value to be 5.9 +/-0.1, and performing high-density fermentation and cultivation for 6.3 hours to obtain bifidobacterium lactis fermentation liquor;
step 2: weighing 15 parts of kudzuvine root, 20 parts of coix seed, 15 parts of Chinese yam, 12 parts of fleece-flower root, 5 parts of common anemarrhena rhizome, 5 parts of liquoric root, 5 parts of donkey-hide gelatin, 9 parts of white paeony root, 15 parts of red bean and 6 parts of hawthorn fruit according to the parts by weight, drying, crushing, sieving and fully mixing to obtain a traditional Chinese medicine preparation B;
and step 3: proportionally mixing 3% of vitamin A, 3% of vitamin D, 10% of vitamin E and 2.5% of vitamin K 3 3.6% of vitamin B 2 Adding a proper amount of water into 4% of carotene and the balance of rice bran meal, fully mixing, and drying to be semi-dry at 50 ℃ to form a paste mixture C;
and 4, step 4: weighing the leaf eating grass powder, the pennisetum hydridum powder, the immortal grass powder, the traditional Chinese medicine preparation B, the paste mixture C and the composite probiotic fermentation broth A according to the weight parts in the table 1, adding a proper amount of water, and uniformly stirring at room temperature to obtain a finished product.
Example 3:
a preparation method of special nutrient containing composite probiotics for feeding chickens comprises the following steps:
step 101: preparing a culture medium A according to 2.13% of beef extract, 3.27% of peptone, 0.50% of yeast powder, 0.83% of wheat root powder, 0.03% of calcium carbonate, 0.025% of magnesium sulfate, 0.025% of manganese sulfate and 12.0% of a composite manganese source, placing clostridium butyricum in the culture medium A containing 1% of manganese sulfate and sterilized at 115 ℃ for 30min, and standing and culturing for 14h in an environment with the temperature of 37 ℃ to obtain a fermentation strain;
step 102: aseptically transferring the fermentation strain to a culture medium A containing 1% manganese sulfate and sterilized at 115 ℃ for 30min, cooling, stirring and fermenting at a stirring speed of 25.0r/min, controlling the pH value to be 7.0 +/-0.2 by feeding alkali liquor, and culturing for 12h to obtain a primary fermentation strain;
step 103: aseptically transferring the primary fermentation strain to a culture medium A containing a composite manganese source and sterilized at 115 ℃ for 30min, standing and fermenting at 37 ℃ for 24h, and obtaining clostridium butyricum fermentation liquor when the residual sugar content is less than or equal to 0.3-0.7%;
step 104: 3.5% of lactose, 1.31% of soybean peptone, 0.88% of yeast peptone, 1.31% of yeast powder and 0.11% of Na 2 HPO 4 0.3% of NaH 2 PO 4 0.43 percent of sodium citrate, 0.04 percent of MgSO 4 0.012% of MnSO 4 Preparing a culture medium B from 0.1% of tween-80, 0.05% of L-cysteine hydrochloride and 12.00% of compound inorganic calcium source, aseptically transferring the bifidobacterium lactis to the culture medium B containing 1% of calcium carbonate in a 5L fermentation tank which is sterilized at 115 ℃ for 30min and cooled, stirring and fermenting at a stirring speed of 80r/min, controlling the pH value to be between 5.9 +/-0.1 by feeding 25% of ammonia water under the environment of 37 ℃, and culturing for 6.3h by high-density fermentation to obtain bifidobacterium lactis fermentation liquor;
step 2: weighing 15 parts of kudzuvine root, 20 parts of coix seed, 15 parts of Chinese yam, 12 parts of fleece-flower root, 5 parts of common anemarrhena rhizome, 5 parts of liquoric root, 5 parts of donkey-hide gelatin, 9 parts of white paeony root, 15 parts of red bean and 6 parts of hawthorn fruit according to the parts by weight, drying, crushing, sieving and fully mixing to obtain a traditional Chinese medicine preparation B;
and step 3: proportionally mixing 3% of vitamin A, 3% of vitamin D, 10% of vitamin E and 2.5% of vitamin K 3 3.6% of vitamin B 2 Adding a proper amount of water into 4% of carotene and the balance of rice bran meal, fully mixing, and drying to be semi-dry at 50 ℃ to form a paste mixture C;
and 4, step 4: weighing the leaf eating grass powder, the pennisetum hydridum powder, the immortal grass powder, the traditional Chinese medicine preparation B, the paste mixture C and the composite probiotic fermentation broth A according to the weight parts in the table 1, adding a proper amount of water, and uniformly stirring at room temperature to obtain a finished product.
Example 4:
a preparation method of special nutrient containing composite probiotics for feeding chickens comprises the following steps:
step 101: preparing a culture medium A according to 2.13% of beef extract, 3.27% of peptone, 0.50% of yeast powder, 0.83% of wheat root powder, 0.03% of calcium carbonate, 0.025% of magnesium sulfate, 0.025% of manganese sulfate and 12.0% of a composite manganese source, placing clostridium butyricum in the culture medium A containing 1% of manganese sulfate and sterilized at 115 ℃ for 30min, and standing and culturing for 14h in an environment with the temperature of 37 ℃ to obtain a fermentation strain;
step 102: aseptically transferring the fermentation strain to a culture medium A containing 1% manganese sulfate and sterilized at 115 ℃ for 30min, cooling, stirring and fermenting at a stirring speed of 25.0r/min, controlling the pH value to be 7.0 +/-0.2 by feeding alkali liquor, and culturing for 12h to obtain a primary fermentation strain;
step 103: aseptically transferring the primary fermentation strain to a culture medium A containing a composite manganese source and sterilized at 115 ℃ for 30min, standing and fermenting at 37 ℃ for 24h, and obtaining clostridium butyricum fermentation liquor when the residual sugar content is less than or equal to 0.3-0.7%;
step 104: 3.5% of lactose, 1.31% of soybean peptone, 0.88% of yeast peptone, 1.31% of yeast powder and 0.11% of Na 2 HPO 4 0.3% of NaH 2 PO 4 0.43 percent of sodium citrate, 0.04 percent of MgSO 4 0.012% of MnSO 4 Preparing a culture medium B from 0.1% of tween-80, 0.05% of L-cysteine hydrochloride and 12.00% of compound inorganic calcium source, aseptically transferring the bifidobacterium lactis to the culture medium B containing 1% of calcium carbonate in a 5L fermentation tank which is sterilized at 115 ℃ for 30min and cooled, stirring and fermenting at a stirring speed of 80r/min, controlling the pH value to be between 5.9 +/-0.1 by feeding 25% of ammonia water under the environment of 37 ℃, and culturing for 6.3h by high-density fermentation to obtain bifidobacterium lactis fermentation liquor;
step 2: weighing 15 parts of kudzuvine root, 20 parts of coix seed, 15 parts of Chinese yam, 12 parts of fleece-flower root, 5 parts of common anemarrhena rhizome, 5 parts of liquoric root, 5 parts of donkey-hide gelatin, 9 parts of white paeony root, 15 parts of red bean and 6 parts of hawthorn fruit according to the parts by weight, drying, crushing, sieving and fully mixing to obtain a traditional Chinese medicine preparation B;
and step 3: proportionally mixing 3% of vitamin A, 3% of vitamin D, 10% of vitamin E and 2.5% of vitamin K 3 3.6% of vitamin B 2 Adding a proper amount of water into 4% of carotene and the balance of rice bran meal, fully mixing, and drying to be semi-dry at 50 ℃ to form a paste mixture C;
and 4, step 4: weighing the leaf eating grass powder, the pennisetum hydridum powder, the immortal grass powder, the traditional Chinese medicine preparation B, the paste mixture C and the composite probiotic fermentation broth A according to the weight parts in the table 1, adding a proper amount of water, and uniformly stirring at room temperature to obtain a finished product.
Table 1: the formula of the special nutrient for feeding chicken in the embodiment 1-4 in parts by weight
The special nutrient for the raised chickens prepared in the above examples 1 to 4 and the special nutrient for the raised chickens sold in the comparative examples are respectively used for feeding chicks with an average body weight of 40.2g within 50 days, the average group is 5, the test period is 8 weeks, the performance of the special nutrient for the raised chickens containing the composite probiotics on the promotion of the growth of the chicks, the reduction of the incidence probability, the improvement of the utilization rate of the feed and the physique of the breeding hens is examined, and the test results are shown in table 2.
Table 2: special nutrient containing composite probiotics for feeding chicken and comparative example result
The table shows that the special nutrient for the raised chickens, which is provided by the application, can promote the absorption of calcium, manganese and iron of the chickens, regulate intestinal flora, convert trace elements of calcium and manganese into calcium lactate, calcium citrate, manganese lactate, manganese alginate and the like which are easy to absorb, enhance the physique of the chickens, prevent wing droop caused by lack of manganese and calcium elements, improve the physique of the chickens and improve the micro-ecological environment in the chickens, greatly reduce the morbidity of the chickens in a stress environment, increase the daily gain of the body weight of the chickens is more than or equal to 16g, promote the growth of the chickens, does not contain any antibiotic product, and has important significance for guaranteeing the health of the chickens.
The above description is only exemplary of the present application and should not be taken as limiting the present application, as any modification, equivalent replacement, or improvement made within the spirit and principle of the present application should be included in the protection scope of the present application.
Claims (10)
1. The special nutrient containing composite probiotics for raising chickens is characterized by comprising the following components in parts by weight: 150-180 parts of lysimachia powder, 50-80 parts of pennisetum hydridum powder, 40-50 parts of immortal grass powder, 8-10 parts of Chinese medicinal preparation, 6-8 parts of compound vitamin and 10-30 parts of compound probiotic fermentation broth; the composite probiotic fermentation liquor is prepared by carrying out liquid submerged fermentation on clostridium butyricum and bifidobacterium lactis.
2. The special nutrient containing complex probiotics for feeding chickens of claim 1, which is characterized in that: the clostridium butyricum fermentation liquor contains 9.5 to 18.5 percent of trace element manganese; the bifidobacterium lactis fermentation liquor contains 5.5-14.0% of trace element calcium.
3. The special nutrient containing complex probiotics for feeding chickens of claim 2, characterized in that: the ratio of the clostridium butyricum fermentation liquor to the bifidobacterium lactis fermentation liquor is 1: 1.
4. The special nutrient containing complex probiotics for feeding chickens of claim 1, which is characterized in that: the vitamin complex comprises the following components in percentage by weight: 3-5% of vitamin A, 2-3% of vitamin D, 10-12% of vitamin E and 2-3% of vitamin K 3 3-5% of vitamin B 2 2-6% of carotene and the balance of rice bran meal.
5. The special nutrient containing the composite probiotics for the raised chickens, as claimed in claim 1, is characterized in that the traditional Chinese medicine preparation comprises the following components in parts by weight: 15 parts of kudzu root, 20 parts of coix seed, 15 parts of Chinese yam, 12 parts of fleece-flower root, 5 parts of rhizoma anemarrhenae, 5 parts of liquorice, 5 parts of donkey-hide gelatin, 9 parts of white paeony root, 15 parts of phaseolus calcaratus and 6 parts of hawthorn.
6. The method for preparing the special nutrient containing the composite probiotics for the raised chickens, which is characterized by comprising the following steps:
step 1: preparing a composite probiotic fermentation liquor A for later use;
step 2: weighing radix Puerariae, Coicis semen, rhizoma Dioscoreae, Polygoni Multiflori radix, rhizoma anemarrhenae, Glycyrrhrizae radix, colla Corii Asini, radix Paeoniae alba, semen Phaseoli and fructus crataegi according to weight parts, drying, pulverizing, sieving, and mixing to obtain Chinese medicinal preparation B;
and step 3: proportionally mixing vitamin A, vitamin D, vitamin E and vitamin K 3 Vitamin B 2 Adding appropriate amount of water into carotene and rice bran meal, mixing, and baking at 50-60 deg.C to semi-dry to obtain paste mixture C;
and 4, step 4: weighing the leaf eating grass powder, the pennisetum hydridum powder, the immortal grass powder, the traditional Chinese medicine preparation B, the paste mixture C and the composite probiotic fermentation liquor A according to the weight parts, adding a proper amount of water, and uniformly stirring at room temperature to obtain a finished product.
7. The method for preparing special nutrient containing composite probiotics for feeding chickens according to claim 6, wherein the method for preparing clostridium butyricum fermentation liquor in step 1 comprises the following steps:
step 101: placing Clostridium butyricum in culture medium A containing 1% manganese sulfate and sterilized at 115 deg.C for 30min, and standing at 35-40 deg.C for 12-16h to obtain fermentation strain;
step 102: aseptically transferring the fermentation strain to a culture medium A containing 1% manganese sulfate and sterilized at 115 ℃ for 30min, cooling, stirring and fermenting at a stirring speed of 15.0-25.0r/min, controlling the pH value to be 7.0 +/-0.2 by adding alkali liquor, and culturing for 12-14h to obtain a primary fermentation strain;
step 103: aseptically transferring the first-stage fermentation strain to a culture medium A containing 6.0-12.0% of composite manganese source and sterilized at 115 deg.C for 30min, standing and fermenting at 37 deg.C for 24 hr until the residual sugar content is less than or equal to 0.3-0.7%.
8. The method for preparing the special nutrient containing the composite probiotics for the raised chickens, which is characterized in that the culture medium A is an MRS culture medium and comprises the following components in percentage by weight: 2.0-5.0% of beef extract, 2.12-4.17% of peptone, 0.5-1.5% of yeast powder, 0.5-1.5% of wheat root powder, 0.01-0.1% of calcium carbonate, 0.025-0.05% of magnesium sulfate, 0.025-0.05% of manganese sulfate and 6.0-12.0% of composite manganese source, wherein the composite manganese source is one or more of manganese sulfate and manganese alginate.
9. The method for preparing special nutrient containing composite probiotics for feeding chickens according to claim 6, wherein the method for preparing the bifidobacterium lactis fermentation liquor in the step 1 comprises the following steps:
step 104: inoculating Bifidobacterium lactis to culture medium B containing 1% calcium carbonate in 5L fermenter sterilized at 115 deg.C for 30min and cooled, stirring at 80r/min for fermentation, adding 25% ammonia water at 37 deg.C to control pH to 5.9 + -0.1, and fermenting at high density for 6.3-7.5 h.
10. The method for preparing the special nutrient containing the composite probiotics for the raised chickens, which is characterized in that the culture medium B comprises the following components in percentage by weight: lactose 3.5%, soybean peptone 1.31%, yeast peptone 0.88%, yeast powder 1.31%, and Na 2 HPO 4 0.11% of NaH 2 PO 4 0.3 percent of sodium citrate, 0.43 percent of MgSO 4 0.04% of MnSO 4 0.012 percent, 0.1 percent of Tween-80, 0.05 percent of L-cysteine hydrochloride and 6 to 12 percent of composite inorganic calcium source, wherein the composite inorganic calcium source is one or a plurality of combinations of calcium carbonate and calcium sulfate.
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