CN114829627B - 基于数字微流控平台富集核酸及构建测序文库的方法 - Google Patents
基于数字微流控平台富集核酸及构建测序文库的方法 Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
- C12M1/36—Apparatus for enzymology or microbiology including condition or time responsive control, e.g. automatically controlled fermentors
- C12M1/38—Temperature-responsive control
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
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Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/CN2020/090796 WO2021232186A1 (fr) | 2020-05-18 | 2020-05-18 | Enrichissement d'acide nucléique à base de plate-forme microfluidique numérique et procédés de construction de banque de séquençage |
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| Publication Number | Publication Date |
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| CN114829627A CN114829627A (zh) | 2022-07-29 |
| CN114829627B true CN114829627B (zh) | 2024-04-30 |
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| CN202080088509.XA Active CN114829627B (zh) | 2020-05-18 | 2020-05-18 | 基于数字微流控平台富集核酸及构建测序文库的方法 |
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| WO (1) | WO2021232186A1 (fr) |
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| CN116453602B (zh) * | 2023-02-17 | 2024-04-19 | 祥符实验室 | 一种基于ddPCR与荧光液滴分选富集的DNA数据库随机读取方法 |
| CN117488412A (zh) * | 2023-12-28 | 2024-02-02 | 北京贝瑞和康生物技术有限公司 | 基于数字微流控技术的靶向捕获测序文库全流程构建方法 |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109182092A (zh) * | 2018-10-19 | 2019-01-11 | 苏州德思普生物科技有限公司 | 一种用于核酸检测的微流控芯片及其应用 |
| CN109420532A (zh) * | 2017-09-01 | 2019-03-05 | 京东方科技集团股份有限公司 | 数字微流控基板及其制作方法、数字微流控芯片及方法 |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008143646A2 (fr) * | 2006-11-29 | 2008-11-27 | Canon U.S. Life Sciences, Inc. | Dispositif et procédé pour des tests multiplex numériques de pcr |
| US20120000777A1 (en) * | 2010-06-04 | 2012-01-05 | The Regents Of The University Of California | Devices and methods for forming double emulsion droplet compositions and polymer particles |
| WO2017037078A1 (fr) * | 2015-09-02 | 2017-03-09 | Illumina Cambridge Limited | Systèmes et procédés d'amélioration du comportement des gouttelettes dans des systèmes fluidiques |
| CN105505761A (zh) * | 2015-12-21 | 2016-04-20 | 中国科学院苏州生物医学工程技术研究所 | 一种数字等温核酸检测装置及其检测方法 |
| CN107118955B (zh) * | 2017-05-12 | 2020-03-10 | 京东方科技集团股份有限公司 | 基因测序芯片及基因测序方法 |
| CN107478629A (zh) * | 2017-09-04 | 2017-12-15 | 中国科学院苏州生物医学工程技术研究所 | 一种大面积数字pcr微滴荧光高通量检测装置和方法 |
| CN110551798A (zh) * | 2018-06-04 | 2019-12-10 | 北京致雨生物科技有限公司 | 一种用于数字聚合酶链反应测定的组合物、方法和系统 |
| CN110205242B (zh) * | 2019-06-18 | 2024-04-26 | 苏州锐讯生物科技有限公司 | 一种快速实现数字pcr反应的微流控芯片组件及其应用 |
| CN110885877A (zh) * | 2019-12-11 | 2020-03-17 | 厦门大学 | 基于恒温扩增与基因编辑的数字微流控芯片的核酸检测方法 |
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2020
- 2020-05-18 WO PCT/CN2020/090796 patent/WO2021232186A1/fr active Application Filing
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109420532A (zh) * | 2017-09-01 | 2019-03-05 | 京东方科技集团股份有限公司 | 数字微流控基板及其制作方法、数字微流控芯片及方法 |
| CN109182092A (zh) * | 2018-10-19 | 2019-01-11 | 苏州德思普生物科技有限公司 | 一种用于核酸检测的微流控芯片及其应用 |
Non-Patent Citations (3)
| Title |
|---|
| Hua 等.Anal Chem./Multiplexed Real-Time Polymerase Chain Reaction on a Digital Microfluidic Platform.2010,第82卷(第6期),EXPERIMENTAL，图1-6. * |
| 液滴微流控系统在数字聚合酶链式反应中的应用研究进展.分析化学/液滴微流控系统在数字聚合酶链式反应中的应用研究进展.2016,第44卷(第8期),3.1节. * |
| 陈宇超等.化工学报/阶梯式T 型微通道内液滴、气泡分散规律.2020,第71卷(第1期),2.1节. * |
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| Publication number | Publication date |
|---|---|
| WO2021232186A1 (fr) | 2021-11-25 |
| CN114829627A (zh) | 2022-07-29 |
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