CN114829625B - 检测方法 - Google Patents

检测方法 Download PDF

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Publication number
CN114829625B
CN114829625B CN202080084779.3A CN202080084779A CN114829625B CN 114829625 B CN114829625 B CN 114829625B CN 202080084779 A CN202080084779 A CN 202080084779A CN 114829625 B CN114829625 B CN 114829625B
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China
Prior art keywords
nucleic acid
signal
wells
stranded
target nucleic
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Active
Application number
CN202080084779.3A
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English (en)
Chinese (zh)
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CN114829625A (zh
Inventor
牧野洋一
堀内阳介
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Toppan Inc
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Toppan Printing Co Ltd
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Publication of CN114829625A publication Critical patent/CN114829625A/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6846—Common amplification features

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CN202080084779.3A 2019-12-09 2020-12-07 检测方法 Active CN114829625B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2019-222153 2019-12-09
JP2019222153 2019-12-09
PCT/JP2020/045438 WO2021117667A1 (ja) 2019-12-09 2020-12-07 検出方法

Publications (2)

Publication Number Publication Date
CN114829625A CN114829625A (zh) 2022-07-29
CN114829625B true CN114829625B (zh) 2025-02-25

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CN202080084779.3A Active CN114829625B (zh) 2019-12-09 2020-12-07 检测方法

Country Status (5)

Country Link
US (1) US20220340953A1 (https=)
EP (1) EP4074823B1 (https=)
JP (2) JPWO2021117667A1 (https=)
CN (1) CN114829625B (https=)
WO (1) WO2021117667A1 (https=)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116908265B (zh) * 2023-09-11 2023-12-12 常州先趋医疗科技有限公司 检测核酸lamp扩增产物电化学生物传感器的制备方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1543509A (zh) * 2001-06-18 2004-11-03 ���л�ѧ��ʽ���� 有效检测双链核酸的方法
CN105940096A (zh) * 2014-01-31 2016-09-14 凸版印刷株式会社 生物分子分析试剂盒及生物分子分析方法

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002090538A1 (en) * 2001-05-08 2002-11-14 Eiken Kagaku Kabushiki Kaisha Method of synthesizing nucleic acid
US20060275771A1 (en) * 2003-04-04 2006-12-07 Hideyuki Suzuki Quantitative reagent, method and equipment of substance utilizing fluorescence lifetime
JP2007089446A (ja) * 2005-09-28 2007-04-12 Ebara Corp センス鎖とアンチセンス鎖を含むｐｃｒ増幅産物からいずれか一方の一本鎖核酸を単離する方法
WO2009054474A1 (ja) 2007-10-26 2009-04-30 Toppan Printing Co., Ltd. アレル判定装置及び方法、ならびに、コンピュータプログラム
JP5597939B2 (ja) * 2009-06-01 2014-10-01 凸版印刷株式会社 部分競合型プローブを用いた標的塩基配列の検出方法
EP2971138B1 (en) * 2013-03-15 2020-05-13 Bio-rad Laboratories, Inc. Digital assays with associated targets
WO2015026873A1 (en) * 2013-08-19 2015-02-26 Singular Bio, Inc. Assays for single molecule detection and use thereof
WO2018168819A1 (ja) * 2017-03-14 2018-09-20 凸版印刷株式会社 解析デバイス、解析キット、及び解析システム

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1543509A (zh) * 2001-06-18 2004-11-03 ���л�ѧ��ʽ���� 有效检测双链核酸的方法
CN105940096A (zh) * 2014-01-31 2016-09-14 凸版印刷株式会社 生物分子分析试剂盒及生物分子分析方法

Also Published As

Publication number Publication date
WO2021117667A1 (ja) 2021-06-17
US20220340953A1 (en) 2022-10-27
EP4074823A1 (en) 2022-10-19
JPWO2021117667A1 (https=) 2021-06-17
EP4074823A4 (en) 2023-08-16
JP2025188101A (ja) 2025-12-25
CN114829625A (zh) 2022-07-29
EP4074823B1 (en) 2024-12-25

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